Entries |
Document | Title | Date |
20080199968 | Method of Forming Signal Probe-Polymer - To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on). | 08-21-2008 |
20080213912 | System and Methods for Stretching Polynucleotides - Apparatus and methods are described for achieving uniform stretching of polynucleotides in hybrid electrophoretic-gel, micro-constricting microfluidic channels. Polynucleotides in normally relaxed configurations are driven by an electric field along a microfluidic channel. The polynucleotides thread through a porous gel barrier formed in the channel and extend into a constriction where an electric field gradient exists. The combined action of the gel and field gradient acts to extend the polynucleotide configuration fully for direct linear analysis of the molecule. | 09-04-2008 |
20080227211 | COMPOSITIONS AND METHODS FOR FREE-SOLUTION CONJUGATE NUCLEIC ACID ANALYSIS - The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products. | 09-18-2008 |
20080227212 | PROCESS FOR IDENTIFYING EXISTENCE OF SINGLE NUCLEOTIDE POLYMORPHISM WITHOUT DNA SEQUENCING - A process for detecting the presence of a mutation in an oligonucleotide strand such as a DNA strand from a gene without the need for DNA sequencing is provided. The inventive process provides a rapid pre-test to screen for the presence or absence of a mutation in a target gene of a subject to determine whether laborious sequencing protocols are required to further characterize a mutation. The inventive process provides a rapid screening protocol for identifying and detecting a genetic mutation in a patient who presents with a disease | 09-18-2008 |
20080227213 | RNA targeting compounds and methods for making and using same - Disclosed are RNA targeting compounds having the formula: | 09-18-2008 |
20080233657 | METHODS TO PREDICT THE OUTCOME OF TREATMENT WITH ANTIDEPRESSANT MEDICATION - The invention provides a method for determining the outcome of treatment with an antidepressant medication in a patient. In particular, the invention provides a method of screening patients to identify those patients with a decreased risk of non-response to treatment with antidepressant medication comprising: (a) obtaining a sample of genetic material from the patients, and (b) assaying the sample for the presence of a genotype in the patients which is associated with a decreased risk of non-response to treatment with antidepressant medication, wherein the genotype is characterized by a polymorphism in a HTR2A, GRIK4, BCL2, or a combination thereof. | 09-25-2008 |
20080241950 | Nucleic Acid Sequencing In Free Solution Using Protein Polymer Drag-Tags - The present invention relates to systems, compositions, and methods for nucleic acid sequencing and analysis in free-solution using protein polymer drag-tags. As such, the present invention provides protein-based molecular compositions that find use as drag-tags for use in sequencing and nucleic acid analysis methods and provides systems and methods for automated sequencing and analysis of nucleic acids in free solution. | 10-02-2008 |
20080254548 | Detection of target analytes using particles and electrodes - The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined. | 10-16-2008 |
20080254549 | SYSTEMS AND METHODS FOR DETECTING AND ANALYZING POLYMERS - A detection system and methods for improving the ability of the detection system to recognize labels that are disposed on a polymer. Embodiments of the invention include schemes for selecting emitters and labels used within the system in a manner that allows an increase in the number of distinct labels that can be used together in a system. In other embodiments, the detection system and methods are directed to identifying portions of a detection signal that may be associated with extra labels residing within a detection zone. In other embodiments, the detection system and methods relate to using wide field imaging detectors while reducing out of focus noise contributions to detection signals of the system. Still, other embodiments relate to the use of linear array detectors to detect labels | 10-16-2008 |
20080261319 | METHOD FOR DETECTING PRESENCE OF ARISTOLOCHIA MATERIALS IN HERBAL PRODUCTS AND BOTANICALS - A method for detecting herbs or plants of the | 10-23-2008 |
20080261320 | Noninvasive blood sugar level measuring method - A noninvasive blood sugar level measurement method includes a process of changing a blood sugar level of a biological body, a process of noninvasively measuring biological feature amounts at plural measurement points that are timewise shifted from each other, a process of invasively measuring blood sugar levels of the biological body by the number of times that is lesser than the measurement points of the biological feature amount, a process of obtaining an approximate curve indicating temporal change in the actually measured blood sugar level that is obtained by the invasive measurement, a process of obtaining, using the obtained approximate curve, blood sugar levels (interpolation blood sugar levels) that would be obtained by the invasive measurement at the measurement times when the biological feature amounts are noninvasively measured, and a process of associating the values of the biological feature amounts at the times corresponding to the respective measurement points of the biological feature amounts with the interpolation blood sugar levels at the respective times, thereby forming a correlation table having plural sets of the biological feature amounts and the interpolation blood sugar levels. Therefore, even when the change in the biological feature amount relative to the blood sugar level is not monotonous, it is possible to form a precise correlation table without increasing the number of times of the invasive blood sugar level measurement. | 10-23-2008 |
20080268545 | Method for detecting a target substance - In order to separate a complex of a first probe, a second probe and a target substance from a reaction mixture obtained by reacting the first probe having first particle (such as labeled particle) and a first binding substance (such as a first oligonucleotide) immobilized on the first particle, the second probe having second particle (such as magnetic particle) and a second binding substance (such as a second oligonucleotide) immobilized on the second particle, and the target substance (such as a target nucleic acid) under mild conditions which do not cause destruction or degradation of the complex, the reaction mixture is mixed with a liquid having a specific gravity greater than that of the first particle, less than that of the second particle and less than that of a particle complex composed of the first particle and the second particle, or a liquid having a specific gravity less than that of the first particle, greater than that of the second particle and greater than that of a particle complex composed of the first particle and the second particle, and then allowed to stand undisturbed until a precipitate and a floating substance are formed. | 10-30-2008 |
20080268546 | DETECTION OF DNA HYBRIDIZATION ON SURFACES - A DNA hybridization surface includes a support having a self assembled monolayer on a metallized surface. The self assembled monolayer includes an alkanethiol and a strand of nucleic acids comprising a functional group that binds to the metallized surface. A method for detecting DNA hybridization in a sample includes (a) incubating a DNA hybridization surface with an aqueous sample that includes a fragment of DNA to produce an incubated DNA hybridization surface; (b) rinsing the incubated DNA hybridization surface to produce a rinsed incubated DNA hybridization surface; (c) contacting the rinsed incubated DNA hybridization surface with a liquid crystal; and (d) determining whether the liquid crystal is uniformly anchored on the rinsed incubated DNA hybridization surface. | 10-30-2008 |
20080274557 | Permethylation Of Oligosaccharides - A solid-phase permethylation procedure is described. For example, solid-phase permethylation can be utilized to prepare permethylated linear and branched, neutral and sialylated oligosaccharides, which can be analyzed by MALDI-MS. | 11-06-2008 |
20080274558 | METHOD FOR IDENTIFYING AND SELECTING LOW COPY NUCLEIC SEGMENTS - The present invention relates to a method of identifying low copy nucleic acid segments from within a known nucleic acid sequence and selecting among the identified low copy segments for segments that are thermodynamically suitable for use in hybridization experiments. | 11-06-2008 |
20080280369 | COMPOSITIONS FOR DETECTION AND ANALYSIS OF POLYNUCLEOTIDES USING LIGHT HARVESTING MULTICHROMOPHORES - Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided. | 11-13-2008 |
20080286878 | Systems, Compositions And Methods For Nucleic Acid Detection - The invention relates to stretch measurements of nucleic acids and correlating those measurements to the extent of double- and single-stranded content of a nucleic acid of interest, and to compositions, systems, and devices related thereto. In preferred embodiments, one performs the stretch or elasticity measurements under conditions such that one can determine a nucleic acid sequence or the presence of an oligonucleotide in a sample. | 11-20-2008 |
20080299667 | OPTICAL REPORTER COMPOSITIONS - This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR. | 12-04-2008 |
20080299668 | DETECTION AND QUANTITATION OF CALICHEAMICIN - The present invention provides a method of detecting the presence of the calicheamicin component of a calicheamicin-carrier conjugate in a fluid sample, wherein the calicheamicin is covalently bound to the carrier. A bond between the calicheamicin and carrier is disrupted, the calicheamicin portion is released from the calicheamicin-carrier conjugate and detected. | 12-04-2008 |
20080311668 | Nucleic Acid Detection - Disclosed is the detection of a target nucleic acid sequence in a mixture of different nucleic acids having additional binding sites, by: hybridizing the target nucleic acid sequence with a probe in liquid phase, the probe having a first label. The additional binding sites are hybridized with single stranded nucleic acids having random primary sequences in liquid phase, the different nucleic acids are separated, and the target nucleic acid is detected by using the labeled probe. | 12-18-2008 |
20080311669 | SCREENING SEQUENCE SELECTIVITY OF OLIGONUCLEOTIDE-BINDING MOLECULES USING NANOPARTICLE BASED COLORIMETRIC ASSAY - Disclosed herein are methods of screening sequence selectivity of oligonucleotide-binding molecules using a gold nanoparticle based calorimetric assay. | 12-18-2008 |
20080318328 | Process for oxidizing unfractionated heparins and detecting presence or absence of glycoserine in heparin and heparin products - The invention provides a process for preparing heparin products with a reduced content of glycoserine. A method for detecting glycoserine in preparations of heparin is also provided. | 12-25-2008 |
20080318329 | Liquid crystal condensation of nucleic acid (na) complexes - Upon cooling solutions or melts of nucleic acids or other polymers in which the constituent molecules exhibit a varying tendency to form multi-strand complexes and thus liquid crystal phases, the complex-forming oligomers preferentially separate into the liquid crystal phase and condensing into liquid crystal domains. Because the tendency for liquid crystal phase formation correlates with the ability of the single strands to form duplexes, which in turn, depends on their degree of complementarity, separation of the liquid crystal domains thus constitutes a method for separating these molecules based upon their degree of complementarity. | 12-25-2008 |
20080318330 | Method of standardization of bacterial polysaccharides found in rumen fluid - A process of standardizing the strength of dried rumen bacterial polysaccharides is described. These dried rumen bacterial polysaccharides may then be included into a composition with vitamins, probiotics, minerals, an amino acid and/or a monosaccharide and fed to reduce the effect of diarrhea in young animals. The described mixture when used for the first few days of life is capable of reducing the severity, incidence and mortality from diarrhea. It also results in increased body weight gain. | 12-25-2008 |
20090011513 | METHOD OF DETECTING NUCLEIC ACID BY USING NUCLEIC ACID MICROARRAY - The present invention provides a method of detecting a nucleic acid which is not restricted by the design of the base sequence of a nucleic acid probe. By repeating washing and detection in multiple stages, the present invention can improve the precision of sequence-specific hybridization stepwise and also can ease restrictions in designing the nucleic acid probes, in particular, restrictions on the Tm value (the temperature at which the nucleic acid double strand is dissociated into single strands) or the sequence length of the nucleic acid probes. | 01-08-2009 |
20090011514 | Dna Crosslinking for Primer Extension Assays - Provided herein are compositions and methods for inhibiting false signals associated with mispriming in primer extension assays. | 01-08-2009 |
20090017547 | SYSTEMS AND METHODS FOR DNA COMPUTING USING METHYLATION - The present disclosure is directed to new methods and systems performing flexible and reversible modification of DNA in order to establish the logical value of true or false for a set of clauses. It combines both the biological meaning and experimental procedure with the logical implementation of the basic Boolean operators: OR, AND, and NOT. A feature of methylation logic is the use of the reversibility of DNA methylation of cytosine and adenine. Logic variables can be negated by reversing the DNA methylation status. Four implementation scenarios are described: three of them use methyl-sensitive restriction enzymes and the fourth uses methyl-binding proteins. Encoding can use either single or double stranded DNA. In addition, the disclosure allows for solving a multi-variable SAT problems by implementing a logic circuit. | 01-15-2009 |
20090023221 | Oligonucleotide probes useful for detection and analysis of microrna precursors - The invention relates to ribonucleic acids and oligonucleotide probes useful for detection and analysis of microRNA precursors and their targets. The invention furthermore relates to oligonucleotide probes for detection and analysis of other non-coding RNAs, mRNAs, mRNA splice variants, allelic variants of single transcripts, mutations, deletions, or duplications of particular exons in transcripts, e.g., alterations associated with human disease, such as cancer. | 01-22-2009 |
20090029477 | Ultra High-Throughput Opti-Nanopore DNA Readout Platform - Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises ( | 01-29-2009 |
20090029478 | DETECTION OF TARGET MOLECULES THROUGH INTERACTION WITH PROBES - A method for detecting a target nucleic acid molecule or target nucleic acid molecular complex comprising: (a) contacting two or more probes complementary to the molecule or molecular complex, said molecule or molecular complex being labeled with one or more fluorescent dye molecules of the same dye or labeled with two dyes that are indistinguishable by their emission characteristics in an assay instrument, wherein each probe interacts specifically with a different target nucleic acid sequence or a structure on the molecule or molecular complex; and (b) detecting interaction of the probes with the molecule or molecular complex, said interaction being detected by an increase in fluorescence intensity during a detection interval having a fluorescence intensity above the fluorescence intensity of any individual free probe, wherein molecule or molecular complex is analyzed such that only individual molecules or molecular complexes in contact with a probe are within an interrogation volume and within a detection time interval. | 01-29-2009 |
20090035869 | METHOD AND SYSTEM FOR GENETIC PATTERN RECOGNITION - A method and an system for the detection of nucleic acid oligonucleotides is characterized in that said detection corresponds to a capacitive reading of the melting point of complexes obtained by hybridization of said oligonucleotides and suitably modified molecules of oligonucleotide probes. | 02-05-2009 |
20090042308 | Method for isolating and modifying DNA from blood and body fluids - This invention is related to a method and assay kit for rapidly quantifying global DNA methylation through immobilizing DNA by simple dry-capture on the plastic carrier followed by immunodetection of 5-methylcytosine structure that is the marker of DNA methylation. | 02-12-2009 |
20090047744 | Method for Improving the Characterisation of a Polynucleotide Sequence - A method of identifying at least one characteristic of a target molecule comprises the steps of: (i) converting the at least one characteristic into a signal polynucleotide; and (ii) identifying the signal polynucleotide sequence, thereby identifying the at least one characteristic of the target molecule wherein each signal polynucleotide comprises at least one control sequence that defines a characteristic of the signal polynucleotide, and wherein identification of the control sequence confirms whether the signal polynucleotide sequence has been identified correctly, and, optionally, if the identification is not correct, provides the necessary information to determine what the correct signal polynucleotide sequence should be. | 02-19-2009 |
20090053820 | Two-step nucleic acid testing method using the same sample - The present invention provides a method of testing whether a sequence of interest is contained in a sample nucleic acid or not, comprising performing a simple test on the sample nucleic acid and subjecting the same sample nucleic acid to a close examination when it has been judged that the sequence of interest is contained therein or when it has been judged that the sequence of interest is not contained therein; and a method of testing whether a sequence of interest is contained in a sample nucleic acid in more than a specific amount or not, comprising performing a simple quantitative test on the sample nucleic acid and subjecting the same sample nucleic acid to a close examination when it has been judged that the sequence of interest is contained therein in more than the specific amount. According to these methods, it becomes possible to examine individuals, species, habitats, etc. of organisms with much higher reliability than that in conventional techniques. | 02-26-2009 |
20090053821 | FLUORESCENCE QUENCHING AZO DYES, THEIR METHODS OF PREPARATION AND USE - Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition. | 02-26-2009 |
20090061526 | NUCLEIC ACID SEQUENCING BY SELECTIVE EXCITATION OF MICROPARTICLES - Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams. | 03-05-2009 |
20090061527 | Methods and Devices for Detecting Nucleic Acid Hybridization - The present invention provides novel methods and devices for detecting hybridization of nucleic acids using liquid crystals and cationic surfactants. | 03-05-2009 |
20090068751 | Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes - Dibenzorhodamine compounds having the structure | 03-12-2009 |
20090068752 | Method for Preparing Analysis Sample, Analysis Sample and Sugar Chain Capture Agent - A method for preparing an analysis sample which involves the sugar chain capture step comprising a reaction of capturing a sugar chain and/or a sugar derivative from a biological sample by using a sugar chain capture agent and the excision step comprising excising a compound containing a moiety capturing the sugar chain and/or the sugar derivative from the sugar chain capture agent after the completion of the sugar chain capture reaction and releasing the compound. | 03-12-2009 |
20090081802 | METHOD FOR ENRICHING SHORT-CHAIN NUCLEIC ACIDS - The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides | 03-26-2009 |
20090093062 | Fluorescent Dye Compounds, Conjugates and Uses Thereof - The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof. | 04-09-2009 |
20090137053 | BEAD SET, PRODUCTION PROCESS OF THE BEAD SET, AND METHOD OF USING THE BEAD SET - A bead set is composed of plural subsets of beads. The beads in each subset are each provided with a surface permitting immobilization thereon of a substance that takes part in a predetermined corresponding reaction or interaction. The individual beads in the beat set are provided with different physical elements, respectively, such that the individual beads in the bead can be distinguished into the plural subsets through an analysis of captured images of the individual beads in the bead set by a computer. | 05-28-2009 |
20090155917 | Method for analyzing nucleobases on a single molecular basis - A method is herein presented for analyzing nucleobases on a single molecular basis (a single molecule detection), which comprises scanning a molecular tip chemically modified with the complementary nucleobase on the nucleobases, and measuring the tunneling currents between the scanned nucleobases and the molecular tips with scanning tunneling microscopy. | 06-18-2009 |
20090176311 | SEPARATION OF CONJUGATED AND UNCONJUGATED COMPONENTS - The invention is based on the use of a basic reagent under basic conditions to separate conjugated saccharide from unconjugated components in a sample, e.g. a vaccine, by precipitation of the conjugated saccharide. The invention allows rapid and quantitative separation of conjugated and conjugated components, which may be exploited in analytical methods for quantifying unconjugated saccharide or carrier. Therefore, the separation of conjugated and unconjugated components using the invention may be advantageously combined with a quantitative saccharide or carrier analysis to provide improved quality control for conjugate vaccines. | 07-09-2009 |
20090203148 | Methods and Devices For Detection and Identification of Encoded Beads and Biological Molecules - The invention relates to methods and devices used in the sequencing, separation, detection, and identification of objects and biological molecules. In preferred embodiments, the invention relates to a DNA sequencing system based on cyclic sequencing by synthesis which is performed on beads in three-dimensional vessels and detected using monolithic multicapillary arrays. In other embodiments, the invention relates to a bead comprising two or more luminescent labels coupled to a nucleic acid sequence. In further embodiments, said luminescent labels are quantum dots. | 08-13-2009 |
20090215187 | Methods and Compositions for the Detection and Analysis of Nucleic Acids by Signal Amplification - The present invention relates to a novel integrated PCR-free signal amplification polynucleotide detection system which combines a specific receptor, an optical transducer, and an amplification mechanism. This novel detection system is based on different electrostatic interactions and confirmations between a cationic polythiophene (i.e., polymer 1) and single-stranded or double-stranded polynucleotides (such as ss-DNA or ds-DNA), and the efficient energy transfer between the triplex (complexation between the cationic polythiophene and ds-DNA) and neighboring fluorophores attached to ss-DNA or ds-DNA probes. It is to be understood that in the case of ss-DNA, triplex formation occurs via the hybridization of complementary ss-DNA strands, combined with complexation and with the cationic polythiophene. The present detection system allows for the detection of single nucleotide polymorphisms (SNPs) from samples in only a few minutes, without the need for nucleic acid amplification. | 08-27-2009 |
20090215188 | Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids - The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in:
| 08-27-2009 |
20090239308 | METHOD AND APPARATUS FOR DETERMINING COPY NUMBER VARIATION USING DIGITAL PCR - A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules. | 09-24-2009 |
20090263908 | Liquid crystal condensation of nucleic acid (na) complexes - Upon cooling solutions or melts of nucleic acids or other polymers in which the constituent molecules exhibit a varying tendency to form multi-strand complexes and thus liquid crystal phases, the complex-forming oligomers preferentially separate into the liquid crystal phase and condensing into liquid crystal domains. Because the tendency for liquid crystal phase formation correlates with the ability of the single strands to form duplexes, which in turn, depends on their degree of complementarity, separation of the liquid crystal domains thus constitutes a method for separating these molecules based upon their degree of complementarity. | 10-22-2009 |
20090263909 | TREATMENT OF NUCLEIC ACID - Methods for treating nucleic acid including: (a) providing an alkali environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) removing unwanted reagents or diluents from the treated nucleic acid sample; and (d) carrying out de-sulphonation of the precipitated treated nucleic acid at a temperature from 70° C. to 95° C. by adjusting the precipitated treated nucleic acid to a pH of between 10 and less than 12.5 to remove sulphonate groups present on the treated nucleic acid and obtain a nucleic acid sample substantially free of sulphonate groups. | 10-22-2009 |
20090286325 | METHOD OF DETECTING TARGET NUCLEIC ACID AND CONTAINER USED FOR THE DETECTING METHOD - An object is to provide a method of easily detecting a target nucleic acid with high precision without using a hybridization method and a container used for the detecting method. The method of detecting the target nucleic acid | 11-19-2009 |
20090291505 | Analytical Device for Thermally Treating a Fluid and/or Monitoring a Property Thereof - A device having a protrusion in one of the large side walls of a chamber thereof is disclosed. The device can be used advantageously in reliable analyses in automate procedures. | 11-26-2009 |
20090291506 | PROCESS - A process for the preparation of coated polymer particles containing superparamagnetic crystals, said process comprising reacting surface-functionalized, superparamagnetic crystal-containing polymer particles of diameter less than 0.5 μm with at least one polyisocyanate and at least one diol. | 11-26-2009 |
20090298187 | COMPOSITIONS, METHODS, AND KITS USING SYNTHETIC PROBES FOR DETERMINING THE PRESENCE OF A TARGET NUCLEIC ACID - Compositions, methods, and kits are provided for determining the presence of a target nucleic acid in a sample using synthetic probes. | 12-03-2009 |
20090305426 | AXIAL ILLUMINATION FOR CAPILLARY ELECTROPHORESIS - System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light. | 12-10-2009 |
20090325305 | DETECTABLE THREADING INTERCALATOR - A threading intercalator of general formula I: | 12-31-2009 |
20090325306 | FINGERPRINT ANALYSIS FOR A PLURALITY OF OLIGONUCLEOTIDES - The invention provides a method for evaluating the accuracy of an oligonucleotide sample, specifically a sample containing a variety of oligonucleotides of potentially varying size and sequence. The method provides a fingerprint that can be used to evaluate the accuracy of a multi-oligonucleotide sample whether or not the sample contains differing oligonucleotides that have the same or about the same molecular weight. | 12-31-2009 |
20100009454 | METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY - Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. | 01-14-2010 |
20100015718 | SUBSTRATE FOR ANALYZING COVERAGE OF SELF-ASSEMBLED MOLECULES AND ANALYZING METHOD USING THE SAME - Provided are a substrate for analyzing the coverage of self-assembled molecules and a method for analyzing the coverage of the self-assembled molecules in nanowire and nanochannel patterned on solid surface, solid surface, or bulk solid surface by using the nanoparticles. According to the method, the presence of specific functional groups of self-assembled molecules and the degree of reaction can be analyzed by introducing nanoparticles to a biomaterial immobilization substrate including self-assembled molecules and measuring the number of gold nanoparticles existing on the surface. The substrate for analyzing the coverage of self-assembled molecules includes: a biomaterial immobilization substrate; a self-assembled molecular layer formed on the substrate and having a functional group capable of reacting with an amine group; a capture DNA molecule having an amine group bounded to the self-assembled molecular layer; and a probe DNA molecule bound to the capture DNA molecule and having nanoparticles attached to on the surface. | 01-21-2010 |
20100015719 | NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR - [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. | 01-21-2010 |
20100022015 | Analysis of Mannosamine-containing Capsular Saccharides - Analysis of compositions that include saccharides having mannosamine residues, such as the capsular saccharide of | 01-28-2010 |
20100022016 | POLYAMIDE NUCLEIC ACID DERIVATIVES AND AGENTS, AND PROCESSES FOR PREPARING THEM - The present invention relates to PNA derivatives which carry, at the C terminus, or at both the C and N termini of the PNA backbone, one or more phosphoryl radicals. The phosphoryl radicals carry, where appropriate, one or more labeling groups, groups for crosslinking, groups which promote intracellular uptake, or groups which increase the binding affinity of the PNA derivative for nucleic acids. The invention furthermore relates to a process for preparing the above-mentioned PNA derivatives and to their use as pharmaceuticals or diagnostic agents. | 01-28-2010 |
20100029008 | POLYMERASE-INDEPENDENT ANALYSIS OF THE SEQUENCE OF POLYNUCLEOTIDES - The present invention concerns methods of polymerase independent template directed elongation of polynucleotides, nucleotide building blocks used in these methods as well as the use of the methods and building blocks for the determination of nucleotide sequences, in particular for the determination of SNPs, base modifications, mutations, rearrangements and methylation patterns. | 02-04-2010 |
20100029009 | Methods for Determining Dysregulation of Methylation of Brain Expressed Genes on the X Chromosome to Diagnose Autism Spectrum Disorders - The discovery that alterations in methylation, which can cause one or more genes on the single X chromosome in males to be partially silenced or overexpressed, constitute a predisposition to autism spectrum disorders is generally disclosed herein. These alterations provide the rationale and basis for methods to diagnose autism spectrum disorders. | 02-04-2010 |
20100035353 | PROCESS FOR THE MANUFACTURING OF GLYCOCHIPS - The present invention relates to a process for the manufacturing of solid supports functionalized by saccharide type molecules (glycochips or carbohydrate arrays or alternatively oligosaccharide arrays). The present invention also relates to the glycochips directly obtained by such a manufacturing process and to their use, in particular for biological analysis and especially for the screening of saccharides or proteins such as Hepatocyte Growth Factors (HGFs) or for the study of saccharides/proteins interactions. | 02-11-2010 |
20100055798 | Degenerate Nucleobase Analogs - The present invention relates to novel degenerate nucleobase analogs and degenerate nucleobase oligomers derived therefrom, and methods of using such degenerate nucleobase oligomers. | 03-04-2010 |
20100075430 | SAMPLE PROCESSING UNITS, SYSTEMS, AND RELATED METHODS - Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided. | 03-25-2010 |
20100081204 | NUCELIC-ACID-RESPONSIVE GEL, METHOD FOR PRODUCING SAME, AND USE OF SAME - The present invention provides a nucleic-acid-responsive gel which allows (i) a larger volumetric change through structural design, (ii) adjustment of its recognition ability to recognize a nucleic acid, (iii) improvement of sensitivity, and (iv) flexible design according to, e.g., a sequence of target DNA. The nucleic-acid-responsive gel includes a probe formed of two single-stranded nucleic acids which are hybridized with each other. The probe is fixed within a network structure of a polymer gel. The two single-stranded nucleic acids are bound reversibly with each other. | 04-01-2010 |
20100099197 | Systems for genome selection - Systems, methods, compositions and apparatus relating to genome selection are disclosed. | 04-22-2010 |
20100112710 | METHODS AND COMPUTER SYSTEMS FOR IDENTIFYING TARGET-SPECIFIC SEQUENCES FOR USE IN NANOREPORTERS - The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to coded, labeled probes that are capable of binding to and identifying target molecules based on the probes' label codes. Methods, computers, and computer program products for identifying target-specific sequences for inclusion in the probes are also provided, as are methods of making and using such probes. The probes can be used in diagnostic, prognostic, quality control and screening applications. | 05-06-2010 |
20100112711 | METHOD OF DETECTING AMPLIFICATION OR DELETION IN GENOMIC DNA FRAGMENT - [Problems] To provide a CGH method, in particular a CGH microarray method, enabling detection at an elevated accuracy and elevated sensitivity. | 05-06-2010 |
20100112712 | Modified Oligonucleotides and Applications Thereof - Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof. | 05-06-2010 |
20100112713 | Methods For Detecting Colorectal Diseases And Disorders - The present invention relates to methods and compositions for the detection of biomarkers associated with colorectal diseases and disorders. In preferred embodiments, said colorectal disease is colorectal cancer. In some embodiments, the invention relates to the detection of said biomarkers using non-invasive methods. In further embodiments, the invention relates to the isolation and evaluation of biomarkers residing in feces from a subject at risk for or exhibiting symptoms associated with a colorectal disease or disorder. In still further embodiments, said biomarkers include exfoliated colonocytes. In additional embodiments, mRNA transcripts isolated from said colonocytes and associated with said colorectal diseases and disorders are quantified. | 05-06-2010 |
20100112714 | BIOCHIP - The present invention relates to a biochip for nucleic acid hybridization. The biochip of the present invention comprises a hybridization chamber which is in the form of a cavity, a porous membrane pressed in the hybridization chamber; and at least one first circulation hole and at least one second circulation hole which are communicated with the hybridization chamber so that the reaction solution flows in the at least one first circulation hole and flows out the at least one second circulation hole through the pores of the porous membrane. The hybridization reaction area is increased by flowing the reaction solution through the pores of the membrane, which enable the reaction sensitivity to be increased. The diffusion distance for the reaction molecules is decreased due to the limited inside space of the membrane, and thereby the hybridization time is shortened. | 05-06-2010 |
20100124786 | Novel Nucleotide and Amino Acid Sequences, and Assays and Methods of use Thereof for Diagnosis of Ovarian Cancer - Novel markers for ovarian cancer that are both sensitive and accurate. These markers are overexpressed and/or differentially expressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis, in ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states. | 05-20-2010 |
20100129920 | METHOD AND SYSTEM FOR MEASURING PHYSICAL PARAMETERS WITH A PIEZOELECTRIC BIMORPH CANTILEVER IN A GASEOUS OR LIQUID ENVIRONMENT - A piezoelectric bimorph cantilever is used for determining physical parameters in a gaseous or liquid environment. The sensor works as a driven and damped oscillator. Contrary to common cantilever sensor systems, the piezoelectric film of the bimorph cantilever acts as both a sensor and an actuator. Using at least two resonance mode of the bimorph cantilever, at least two physical parameters can be measured simultaneously in a gas or a liquid. An optimized piezoelectric cantilever and a method to produce the cantilever are also described. | 05-27-2010 |
20100136701 | Device including a dissolvable structure for flow control - A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway. | 06-03-2010 |
20100136702 | METHODS AND COMPOSITIONS FOR DETECTION AND ANALYSIS OF POLYNUCLEOTIDES USING LIGHT HARVESTING MULTICHROMOPHORES - Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided. | 06-03-2010 |
20100151584 | MULTI-DIMENSIONAL CHROMATOGRAPHIC METHODS FOR SEPARATING N-GLYCANS - A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique. | 06-17-2010 |
20100167410 | MASS-SPECTROMETRIC METHOD CARRIED OUT ON SAMPLES CONTAINING NUCLEIC ACIDS - The invention relates to a mass spectrometric method for the detection and for the quantification of double stranded nucleic acids which are not covalently associated with one another. | 07-01-2010 |
20100167411 | NOVEL SLC17-TYPE TRANSPORTER PROTEIN IN MAMMAL AND USE THEREOF - It is an object of the invention to isolate a transporter responsible for ATP transport and a gene encoding the transporter. It is another object of the invention to provide a method for the screening of a medicament for treating and/or regulating pain in central nerves, blood coagulation by platelet-derived ATP, or the like, the method employing such a transporter. According to the invention, a transporter responsible for ATP transport and a gene encoding the transporter were isolated. Furthermore, there is provided a method for the screening of a medicament for treating and/or regulating pain in central nerves, blood coagulation by platelet-derived ATP, or the like, the method employing such a transporter. | 07-01-2010 |
20100184230 | NANOCRYSTALS HAVING POLYNUCLEOTIDE STRANDS AND THEIR USE TO FORM DENDRIMERS IN A SIGNAL AMPLIFICATION SYSTEM - Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complementary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission. | 07-22-2010 |
20100210024 | Methods For Producing Surface Bound Oligonucleotide on Solid Substrate and Uses Thereof - The present invention relates to methods for producing an oligonucleotide on a solid substrate surface and methods for using the same. Some aspects of the invention provide methods for selecting a single DNA molecule reproducibily with an atomic force microscope (AFM). | 08-19-2010 |
20100210025 | Common Module Profiling of Genes - A system for profiling a genomic sequence comprising assigning modules to a genome, wherein each module has a defined sequence characteristic and the genome is divided into modules; assigning a value or weight to a module for a given profile, wherein the presence of one or more modules in a genomic sequence contributes to the profile of the genomic sequence relative to its value or weight; analysing a genomic sequence to identify modules present; and assigning a profile to the genomic sequence based on the presence of the modules and their respective value or weight. | 08-19-2010 |
20100216251 | USE OF ACETIC NITRIC ACID REAGENT FOR EXTRACTION OF OLIGOSACCHARIDES AND POLYSACCHARIDES TO CHARACTERIZE CARBOHYDRATE MATERIALS FROM PLANTS AND OTHER SOURCES OF CELLULOSE USING GLYCAN OLIGOMER ANALYSIS - This patent application is for the use of acetic nitric reagent (80% acetic acid, 1.8 N nitric acid) for the extraction of oligosaccharides and polysaccharides from carbohydrate containing materials The material is extracted with the acetic nitric reagent in a boiling water bath for various periods of time, usually 30 minutes The material is then centrifuged and the clear, yellowish, supernatant is then taken to dryness in a Speed Vac under reduced pressure. The dry residue is then taken up in water and centrifuged to remove particulates The resulting supernatant is then analyzed by high pH anion exchange chromatography with integrated amperonetric detection The resulting chromatogram matogram or the integrated areas under the peaks are then characteristic for that particular source of material. | 08-26-2010 |
20100221841 | SEMICONDUCTOR DNA SENSING DEVICE AND DNA SENSING METHOD - A semiconductor DNA sensing device is provided herein, which includes a detection section with a field-effect transistor including a semiconductor substrate and a first insulator layer formed thereon as a reactive gate insulator, the first insulating layer including silicon oxide or an inorganic oxide, a first organic monolayer formed on the first insulator layer, the first organic monolayer comprising an organic molecule having a reactive functional group, and a probe DNA containing 3 to 35 nucleotides bonded to the first organic monolayer by the reactive functional group either directly or by an intervening crosslinker, the structure of the probe DNA/the first organic monolayer/the insulating layer/the semiconductor constituting the detection section. | 09-02-2010 |
20100233820 | MULTIPURPOSE ANALYSIS USING SECOND HARMONIC GENERATING NANOPROBES - Second harmonic nanoprobes for multipurpose imaging of samples and a method of using such probes to monitor nucleotide sequencing in a Multi-SHG Detection Imaging (MSDI) modality and to monitor external electric field using voltage sensitive second harmonic generating (SHG) nanoprobes are provided. The SHG nanoprobes are comprised of various kinds of nanocrystals that do not possess an inversion symmetry and therefore are capable of generating second harmonic signals that can then be detected by conventional two-photon microscopy for in vivo imaging of biological processes and structures such as cell signaling, neuroimaging, protein conformation probing, DNA conformation probing, gene transcription, virus infection and replication in cells, protein dynamics, tumor imaging and cancer therapy evaluation and diagnosis as well as quantification in optical imaging for a wide-range of biological and non-biological processes and devices. | 09-16-2010 |
20100248379 | OPTICAL SENSOR WITH LAYERED PLASMON STRUCTURE FOR ENHANCED DETECTION OF CHEMICAL GROUPS BY SERS - An optical sensor and method for use with a visible-light laser excitation beam and a Raman spectroscopy detector, for detecting the presence chemical groups in an analyte applied to the sensor are disclosed. The sensor includes a substrate, a plasmon resonance mirror formed on a sensor surface of the substrate, a plasmon resonance particle layer disposed over the mirror, and an optically transparent dielectric layer about 2-40 nm thick separating the mirror and particle layer. The particle layer is composed of a periodic array of plasmon resonance particles having (i) a coating effective to binding analyte molecules, (ii) substantially uniform particle sizes and shapes in a selected size range between 50-200 nm (ii) a regular periodic particle-to-particle spacing less than the wavelength of the laser excitation beam. The device is capable of detecting analyte with an amplification factor of up to 10 | 09-30-2010 |
20100261285 | TAGGED-FRAGMENT MAP ASSEMBLY - A method for determining a sequence of a biomolecule, the method including binding a plurality of uniform probes to a biomolecule fragment, creating a collection of binding signatures for the fragment with each binding signature representing a series of distances between binding sites within the fragment, and grouping the binding signatures into a plurality of signature clusters based at least in part on distances between the binding sites in each binding signature. For each binding signature in a first cluster, a potential successor binding signature is selected from signature clusters other than the first signature cluster, and one of the potential successor binding signatures is identified as a successor binding signature. The last two steps are repeated until the successor signature represents a terminal signature, resulting in a sequence of signatures representing at least a portion of the biomolecule. | 10-14-2010 |
20100267152 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267153 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267154 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267155 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267156 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267157 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 10-21-2010 |
20100267158 | Electronic Detectors Inside Nanofluidic Channels For Detection, Analysis, and Manipulation of Molecules, Small Particles, and Small Samples of Material - The present invention provides methods and apparatus that can manipulate, detect, and/or analyze single molecules, single small particles or single small samples of matter passing through a nanoscale gap within a nanofluidic channel of a detector. | 10-21-2010 |
20100279422 | METHOD OF SURFACE PLASMON RESONANCE (SPR) TECHNOLOGY TO DETECT GENOMIC DISORDERS FOR PRENATAL DIAGNOSIS - The present invention discloses using SPR technology to prenatally detect specific DNA loss or gain related to some genomic disorders. An efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of DNA markers used for the identification of chromosome numerical abnormalities (such as chromosomes 13, 18, 21, X and Y related anomalies) and chromosome microdeletion syndromes (such as DiGeorge syndrome, etc) is also disclosed. | 11-04-2010 |
20100279423 | GENETIC MARKERS OF MENTAL ILLNESS - This invention relates to genetic markers of mental illness, e.g., schizophrenia (SZ) and methods of use thereof. | 11-04-2010 |
20100285598 | Novel Artificial Base Pairs and Uses Thereof - The present invention provides nucleic acids based on novel artificial base pairing, as well as a preparation method and uses thereof. | 11-11-2010 |
20100285599 | Method and Device for the Quantitative Real Time Analysis of Fluorescent Samples - A method for the quantitative real time analysis of fluorescent samples is provided, at which each of the samples is excited to fluoresce by a sample individual light source ( | 11-11-2010 |
20100285600 | Markers associated with the therapeutic efficacy of glatiramer acetate - The present invention is directed to methods and kits based, at least in part, on the identification of allele-specific responsiveness or non-responsiveness to glatiramer acetate for the treatment of autoimmune disorders, such as relapsing-remitting multiple sclerosis. The allele-specific responsiveness or non-responsiveness is based on polymorphisms in the following genes, CTSS, MBP, TCRB, CD95, CD86, IL-1R1, CD80, SCYA5, MMP9, MOG, SPP1 and IL-12RB2. | 11-11-2010 |
20100285601 | METHOD OF ELECTRICALLY DETECTING A NUCLEIC ACID MOLECULE - The method is performed by means of a pair of electrodes that are arranged at a distance and within a sensing zone. A nucleic acid capture molecule with an uncharged backbone and a nucleotide sequence that is at least partially complementary to at least a portion of a strand of the target nucleic acid molecule, is immobilised on an immobilisation unit. The immobilisation unit, which is arranged within the sensing zone, is contacted with a solution suspected to comprise the target nucleic acid molecule, which hybridizes to the nucleic acid capture molecule. An activation agent is added, which has an electrostatic net charge complementary to the net charge of the target nucleic acid molecule. It associates to the complex of nucleic acid capture molecule and target nucleic acid molecule. Added is a water soluble polymer with at least one polymer strand and with an electrostatic net charge that is complementary to the net charge of the activation agent. Thus the polymer associates to the activation agent. A metal salt is added, which can act as an oxidant and the metal ions of which have an electrostatic net charge complementary to the net charge of the polymer. The metal ions associate to the polymer. Upon adding a reducing agent, the latter reduces the metal ions, forming a metal wire. The presence of the analyte molecule is determined based on an electrical characteristic of a region in the sensing zone that is affected by the metal wire. | 11-11-2010 |
20100291695 | DETECTION OF NUCLEIC ACID SEQUENCE MODIFICATION - The invention relates to a non-PCR based method for the detection of a nucleotide modification in a nucleic acid sample comprising contacting a solution comprising nucleic acid sample with a nucleic acid probe in a temperature-controlled and UV illuminated container and measuring the UV absorption of the nucleic acid sample/nucleic acid probe complex. | 11-18-2010 |
20100291696 | Process For Detecting Nucleic Acids - A process for detecting nucleic acids, having the following steps: providing at least one nanoparticle that is functionalised for the nucleic acid to be detected by means of at least one oligonucleotide that is bound to it and that is able to hybridize with at least one segment of a nucleic acid to be detected; bringing the functionalised nanoparticle into contact with a sample in which the nucleic acid is to be detected; and measuring a property that provides information about the degree of hybridization of the at least one oligonucleotide with the nucleic acid to be detected. In addition, the process includes the step of exciting the nanoparticles to generate heat, for example by means of a photothermal effect. The invention is suitable, in particular, for high-throughput DNA analysis. | 11-18-2010 |
20100291697 | Coated Colloidal Materials - Coated colloidal materials, methods for making coated colloidal materials, and methods of using coated colloidal materials are disclosed. The method yields coated colloidal materials where the optical characteristics of the core is not adversely affected. The coated colloidal materials can be self-assembled into films, layers, or structures and used in the detection of analytes through detection assays. | 11-18-2010 |
20100311178 | Labelled nucleotides - The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C | 12-09-2010 |
20100317122 | NEW ULTRA-SENSITIVE CHEMILUMINESCENT SUBSTRATES FOR ENZYMES AND THEIR CONJUGATES - New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent. an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.5 | 12-16-2010 |
20100317123 | Method and Device for Detection of an Analyte - The present invention relates to a method, device and kit for analysing a sample for determining the presence or amount of an analyte, particularly carbohydrate, more particularly sugar, in the sample using a fabric. | 12-16-2010 |
20100323453 | Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology - Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. | 12-23-2010 |
20100330686 | NANOSENSOR FOR SUGAR DETECTION - Nanosensors for carbohydrate detection are disclosed. The nanosensors include a nanoparticle conjugated to one or more boronic acid molecules or derivatives thereof and one or more pH sensitive materials. The nanosensors may be provided on a pH indicator paper in order to quickly assay samples, such as food samples. | 12-30-2010 |
20110003394 | ENCODED MICROPARTICLES - An encoded microparticle including an optical substrate comprising a material that permits light to propagate therethrough. The optical substrate has an elongated body that extends in a direction along a central axis. The optical substrate includes an outer region that extends about the central axis. The encoded microparticle also includes an optically detectable code that is disposed within the optical substrate and extends along the central axis. The outer region surrounds the optically detectable code about the central axis. The optically detectable code is readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code. Said at least one of reflected or filtered light propagates through the outer region to be detected for reading the optically detectable code. | 01-06-2011 |
20110008905 | SYSTEM FOR DETECTING POLYNUCLEOTIDES - The present invention relates to methods for detecting the presence or amount of a target polynucleotide. A polynucleotide, target nucleic acid analog, and dye are combined to form a mixture. The optical property of the dye is observed after the mixture is exposed to a stimulating means. Optionally, after the stimulating means is employed, the mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar mixture containing a known amount of a target polynucleotide/nucleic acid analog hybrid to determine a relative rate of change in the optical property. The change in a property of the mixture after exposure thereof to a stimulating means or the relative rate of change in the optical property of dye in the mixture is correlated with the presence or amount of the specified target polynucleotide in the sample. | 01-13-2011 |
20110008906 | Genetic Association of Polymorphisms in Perilipin (PLIN) Gene With Resistance to Weight Loss - Diagnostics and therapeutics for resistance to weight-loss, which are based upon the identification of a subject's PLIN polymorphisms, haplotype and genotype pattern, are described in this invention. | 01-13-2011 |
20110014715 | Synthesis of Peptide Nucleic Acids Conjugated with Amino Acids and Their Application - This invention relates to a peptide nucleic acid (PNA) oligomer which is conjugated with one or more linear-type amino acid containing a plurality of alkyleneglycols and to a synthesis method thereof. In addition, this invention related to a linear amino acid spacer in a device for detection for detecting a target gene using the PNA oligomers which is fixed on a surface of a functionalized solid support. The linear amino acid spacer contains a plurality of alkyleneglycols and maintains enough space between the solid support and PNA oligomer in the device in order to prevent the interference of the interaction between the PNA oligomer and a target gene. Furthermore, this invention relates to a PNA array, a PNA chip and a gene diagnosis kit whereof sensitivity and specificity are improved by being manufactured with the PNA conjugated with the amino acid spacer. | 01-20-2011 |
20110014716 | COMPOSITIONS AND METHODS FOR DIAGNOSING AND TREATING MACULAR DEGENERATION - The present invention relates generally to biomarkers for macular degeneration. In particular, the present invention provides a plurality of biomarkers for monitoring and diagnosing macular degeneration. The compositions and methods of the present invention find use in diagnostic, therapeutic, research, and drug screening applications. | 01-20-2011 |
20110027898 | METHODS AND ARTICLES FOR STRAND-SPECIFIC POLYNUCLEOTIDE DETECTION WITH CONJUGATED POLYMERS - The invention further relates to conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer with the polymer may occur upon binding of the sensor and the target. Other variations of the inventions are described further herein. | 02-03-2011 |
20110039344 | METHOD AND TESTING A SUBJECT THOUGHT TO BE PREDISPOSED TO LUNG CANCER - The present invention concerns a method of testing a human thought to be predisposed to having lung cancer which comprises the step of analyzing a biological sample from said human for detecting the presence of a polymorphism on chromosome 15q25 associated with lung cancer. | 02-17-2011 |
20110039345 | SYSTEM AND METHOD FOR ELECTROCHEMICAL DETECTION OF BIOLOGICAL COMPOUNDS - The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reversibly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals. | 02-17-2011 |
20110053281 | Genetic variants on CHR 11Q and 6Q as markers for prostate and colorectal cancer predisposition - It has been discovered that certain polymorphic markers on chromosome 6 and chromosome 11 are indicative of a susceptibility to prostate cancer and colon cancer. The invention describes diagnostic applications for determining a susceptibility to cancer using such markers, as well as kits for use in such applications. | 03-03-2011 |
20110053282 | Methods and Kits Using Extended Rhodamine Dyes - Extended rhodamine compounds exhibiting favorable fluorescence characteristics having the structure | 03-03-2011 |
20110059541 | Method for Obtaining Information on Formation of Double-Stranded Nucleic Acid - Provided is a method for obtaining information on the formation of a double-stranded nucleic acid by detecting fluorescence, which is simple, is applicable to various interaction systems, and features high detection sensitivity with reduced background signals. Specifically provided is a method for obtaining information on the formation of a double-stranded nucleic acid, which includes detecting information on fluorescence of a probe consisted of a labeling fluorescent dye labeled on a nucleic acid strand and an intercalator bound or inserted between base pairs in the double-stranded nucleic acid to permit an energy transfer with the fluorescent dye. | 03-10-2011 |
20110059542 | DETECTION OF NUCLEIC ACIDS BY TARGET-SPECIFIC HYBRID CAPTURE METHOD - Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods. | 03-10-2011 |
20110104814 | HEPARAN SULFATE GLYCOSAMINOGLYCAN LYASE AND USES THEREOF - The invention provides recombinant | 05-05-2011 |
20110111514 | LABELLING REAGENTS HAVING A PYRIDINE NUCLEUS BEARING A DIAZOMETHYL FUNCTION, PROCESS FOR SYNTHESIS OF SUCH REAGENTS AND PROCESSES FOR DETECTION OF BIOLOGICAL MOLECULES - A process for synthesis of a labelling reagent, a process for the labelling of a biological molecule, a labelled biological molecule obtained by the process, a process for labelling and fragmentation of a single or double strand nucleic acid, a labelled nucleic acid capable of being obtained by the process, a kit for detection of a target nucleic acid containing a labelled nucleic acid, a solid support onto which is attached a reagent and a process for capture of nucleic acids. | 05-12-2011 |
20110117660 | PHYSICAL CHARACTERIZATION OF OLIGONUCLEOTIDE CONJUGATES - The present invention relates generally to methods useful in characterizing oligonucleotide conjugates, comprising an oligonucleotide and a modifying high molecular weight compound, by providing means for separating the high molecular weight compound from the oligonucleotide while maintaining the oligonucleotide's structural integrity. The ability to break the oligonucleotide construct down into its constituent parts while maintaining the structural integrity of the oligonucleotide allows for more efficient and effective downstream analysis including, but not limited to, sequencing of the oligonucleotide, characterization of degradation products, and identification of areas of decreased stability in the oligonucleotide conjugate. | 05-19-2011 |
20110124111 | LOW-VOLUME SEQUENCING SYSTEM AND METHOD OF USE - Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein. | 05-26-2011 |
20110129938 | L-FUCOSE ALPHA1-6 SPECIFIC LECTIN - Disclosed is a novel lectin which can bind specifically to an L-fucose α1→6 sugar chain. Also disclosed is use of the lectin. The L-fucose α1→6 specific lectin of the present invention is characterized in that: (1) the lectin is extracted from a basidiomycete or an ascomycete; (2) the lectin has a molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 4,000 to 40,000; and (3) the lectin has an affinity to an L-fucose α1→6 sugar chain, the affinity being represented by an association constant of 1.0×10 | 06-02-2011 |
20110136245 | SYNERGISTIC INTERACTIONS OF PHENOLIC COMPOUNDS FOUND IN FOOD - Synergistic nutritional supplements of multiple antioxidant compounds with ratios derived from the ratios in naturally occurring foodstuffs. | 06-09-2011 |
20110136246 | NOVEL ONCOGENE NRF2 - The present invention provides a marker which can be used as an indicator for efficacy prediction of an mTOR related anticancer agent or prognostic prediction, and a novel anticancer agent. The present invention provides a method for efficacy evaluation of a cancer drug, and, specifically, a prediction method for the efficacy of an mTOR-related cancer drug by detecting NRF2 abnormality. In addition, the present invention provides a prognostic prediction method for cancer, and, specifically, a prediction method for the prognosis of cancer by detecting NRF2 abnormality. Furthermore, the present invention provides a novel anticancer agent that targets NRF2. | 06-09-2011 |
20110143446 | Axial Illumination for Capillary Electrophoresis - System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light. | 06-16-2011 |
20110159601 | STUDY OF POLYMER MOLECULES AND CONFORMATIONS WITH A NANOPORE - The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer. | 06-30-2011 |
20110159602 | ANALYSIS OF VI SACCHARIDES - Vi saccharide can be assayed in two new ways. First, its proton NMR spectrum can be used, with comparison to an internal Standard permitting quantitative analysis. Second, anion exchange chromatography with amperometric detection can be used on hydrolysed saccharide. | 06-30-2011 |
20110165690 | METHOD FOR DIAGNOSING DISEASE USING ADENOVIRUS HARBORING TRANS-SPLICING RIBOZYME BY MOLECULAR IMAGING - Disclosed herein is a composition for molecular imaging comprising a trans-splicing ribozyme coupled with an imaging reporter gene. The trans-splicing ribozyme targets a specific gene associated with a disease. Also disclosed is a molecular imaging method using the composition. | 07-07-2011 |
20110165691 | AZIDE SUBSTITUTED NAPHTHYLENE OR RYLENE IMIDE DERIVATIVES AND THEIR USE AS REAGENTS IN CLICK-REACTIONS - Novel mono-azide substituted rylene-imide derivatives, their use in methods for the detection of analytes and reagents kits for the detection of analytes comprising said novel mono-azide substituted rylene-imide derivatives. | 07-07-2011 |
20110171740 | BIOASSAYS BASED ON POLYMERIC SEQUENCE PROBE - Polymeric sequence probes and methods are described that enhance the speed and sensitivity of detection of target analytes by combining a multiplicity of binding moieties specific for analyte, at least two of which are linearly arranged and optionally a multiplicity of detectable labels. | 07-14-2011 |
20110171741 | DNA INTEGRITY ASSAY (DIA) FOR CANCER DIAGNOSTICS, USING CONFOCAL FLUORESCENCE SPECTROSCOPY - The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer. | 07-14-2011 |
20110189781 | DISTANCE-CONTROLLED ENERGY TRANSFER DYE COMPLEXES - The invention relates to the use of at least two different fluorophores for configuring a fluorescence resonance energy transfer pair (FRET pair), wherein at least one first fluorophore (A) serves as the donor fluorophore and at least one second fluorophore (B) serves as the acceptor fluorophore within the FRET pair, wherein the first fluorophore (A) and the second fluorophore (B), independently of each other, each are configured on the basis of an organo-metal complex of rare earth elements, wherein the fluorophores (A) and (B) comprise different rare earth elements from each other. | 08-04-2011 |
20110189782 | APPARATUS AND METHODS FOR PROCESSING BIOLOGICAL SAMPLES AND A RESERVOIR THEREFOR - An apparatus for processing at least one biological sample accommodated on at least one carrier member ( | 08-04-2011 |
20110195512 | NOVEL PROTEIN CAPABLE OF BINDING TO HYALURONIC ACID, AND METHOD FOR MEASUREMENT OF HYALURONIC ACID USING THE SAME - The present invention relates to a polynucleotide encoding a protein comprising an amino acid sequence shown in SEQ ID NO: 2, wherein the protein encoded by the polynucleotide has a hyaluronic acid binding ability, the protein, a method for measuring hyaluronic acid using the protein, and a reagent kit for measuring hyaluronic acid comprising the protein as a constituent. | 08-11-2011 |
20110223676 | DNA-BASED MOLECULAR SWITCHES AND USES THEREOF - Disclosed are nucleic acid-based molecular switches that respond to changes in pH. The switches may be used in DNA nanodevices. The switches may also act as sensors for measuring the pH of a sample, including cells, regions thereof, and whole organisms. The switch includes an A-motif that forms at acidic pH. Also disclosed are compositions and methods for measuring the pH of cells or regions thereof, such as vesicles, the nucleus, mitochondrial matrix, or the Golgi lumen. | 09-15-2011 |
20110223677 | CARBOXAMIDE-SUBSTITUTED DYES FOR ANALYTICAL APPLICATIONS - The present invention relates to carboxamide-substituted dyes, the production and use of such dyes as labeling groups in analytics. | 09-15-2011 |
20110229975 | Hybridization Compositions and Methods - The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences. | 09-22-2011 |
20110229976 | Sequencing of nucleic acid molecules by mass spectrometry - The present invention is related to a method for determining the nucleotide sequence of a nucleic acid molecule comprising the following steps:
| 09-22-2011 |
20110236983 | SINGLE MOLECULE DETECTION AND SEQUENCING USING FLUORESCENCE LIFETIME IMAGING - A nucleic acid detection system and method are provided, in which excitation energy is transmitted from a pulsed excitation source to a reaction site including a fluorescence resonance energy transfer (FRET)-based dye system to generate a fluorescent signal at the reaction site, the fluorescent signal is detected by a detector from the reaction site, and detection of the fluorescent signal is respectively blocked and permitted at the detector by a detector gate this is timed based on an emission start time of the transmitted excitation energy. | 09-29-2011 |
20110236984 | DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME - In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand. | 09-29-2011 |
20110250697 | NON-NATURALLY OCCURRING DNA SEQUENCES - DNA compositions having nucleotide sequences that do not occur in nature. Also provided are fluorescently labeled DNA molecules and complementary DNA on solid substrates, such as microspheres. | 10-13-2011 |
20110250698 | METHODS OF NUCLEIC ACID TARGET CAPTURE - Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed. | 10-13-2011 |
20110250699 | Fluorescent Dye Compounds, Conjugates and Uses Thereof - The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof. | 10-13-2011 |
20110256631 | METHODS AND PROCESSES FOR CALLING BASES IN SEQUENCE BY INCORPORATION METHODS - Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof. | 10-20-2011 |
20110281368 | NANOPARTICLE DERIVATIZATION OF TARGETS FOR DETECTING AND DETERMINING THE CONCENTRATIONS OF TARGETS BY IMPEADANCE-SPECTROSCOPY-BASED SENSORS - Embodiments of the present invention are directed to for detecting the presence and concentration of one or more particular target molecules in solutions, air or other gasses, or otherwise present in an environment or sample, by impedance-spectroscopy-based sensors. Various embodiments of the present invention provide for derivatizing target molecules with nanoparticles to increase capacitance changes at electrode surfaces in order to generate stronger signals and improve signal-to-noise ratios of impedance-spectroscopy-based sensors. | 11-17-2011 |
20110281369 | ANALYSIS OF MYCOPHENOLIC ACID IN SALIVA USING LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY - A method for mass spectrometric analysis of a saliva sample possibly containing mycophenolic acid or its metabolites mycophenolic acid phenyl glucuronide (MPAG) or mycophenolic acid acyl-glucuronide (Acyl-MPAG), including the steps: (a) providing a saliva sample containing one or more drug or metabolites; (b) deproteinating the sample; (c) separating the one or more drug or metabolites from the saliva sample; and (d) analyzing the one or more drug or metabolites using a mass spectrometer. The sample containing one or more MPA or metabolites is obtained from in an oral fluid based biological samples i.e. whole saliva or saliva obtained by chemical or mechanical stimulation or from specific salivary glands. The size of the sample contains one or more MPA or metabolites is at least about 100 microL. A kit for use in mass spectrometric analysis of a sample may contain one or more MPA or metabolites from saliva samples, comprising: (a) reagents for deproteinating of the saliva sample, including internal standards; (b) reagents for separating the one or more MPA or metabolites from the saliva sample; (c) reagents for analyzing the one or MPA or metabolites using a mass spectrometer; (d) a solution of one or more MPA or metabolites in saliva samples; and (e) instructions for analyzing the one or more MPA or saliva using a mass spectrometer. The kit includes (a) mobile phase solutions; (b) a chromatography column; and (c) a quality control specimen. | 11-17-2011 |
20110287550 | Method for continuously monitoring solution-phase synthesis of oligonucleotide - The present invention provides a system and method for real-time continuously monitoring of oligonucleotide synthesis in solution phase. | 11-24-2011 |
20110306143 | Apparatus for single-molecule detection - An apparatus for detecting an object capable of emitting light. The apparatus includes a light source and a waveguide. The waveguide includes a core layer and a first cladding layer. At least one nanowell is formed in at least the first cladding layer. The apparatus further includes a light detector. The light detector can detect a light emitted from a single molecule object contained in the at least one nanowell. | 12-15-2011 |
20120009686 | METHOD OF MEASURING THE CONCENTRATION OF A GLYCOSAMINOGLYCAN ANTICOAGULANT - The invention provides an accurate, economical, automatable, high throughput method for the determination of the concentration of glycosaminoglycan anticoagulants, including low molecular weight heparin (LMWH) anticoagulants, in aqueous solutions. A method for cleaning a unit of manufacturing equipment used in the preparation of a LMWH to obtain an acceptable residual concentration of LMWH is further provided. | 01-12-2012 |
20120015442 | MICROFLUIDIC SYSTEM INCLUDING A BUBBLE VALVE FOR REGULATING FLUID FLOW THROUGH A MICROCHANNEL - A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane. | 01-19-2012 |
20120021525 | OPTICS COLLECTION AND DETECTION SYSTEM AND METHOD - Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described. | 01-26-2012 |
20120058566 | DETECTION OF IMMOBILIZED NUCLEIC ACID - The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures. | 03-08-2012 |
20120064633 | FLUORESCENCE QUENCHING AZO DYES, THEIR METHODS OF PREPARATION AND USE - Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition. | 03-15-2012 |
20120070902 | METHOD FOR ANALYSIS OF SUGAR CHAINS BY MASS SPECTROMETRY - Provided is a highly accurate means for analyzing a sugar chain contained in a biological sample by MALDI-TOF MS or such a mass spectrometry method. In the present invention, it was found to be possible to improve the ionization efficiency of a sugar chain by methylating the hydroxyl groups of the sample sugar chain before conducting the MALDI-TOF MS analysis, and by so doing to carry out quantitative analysis and structural analysis of the sample sugar chain with high accuracy. | 03-22-2012 |
20120077276 | METHODS OF DETERMINING POTENCY OF CHEMICALLY-SYNTHESIZED OLIGONUCLEOTIDES - Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2. | 03-29-2012 |
20120083038 | Methods for Detecting the Presence or Absence of Blood - The present invention relates to methods for detecting presence, or absence, of blood. Specifically, the present invention relates to methods for detecting the presence, or absence, of blood, comprising applying a solution comprising luminol, or luminol derivative, a base, an oxidizing agent and fluorescein, or a fluorescein derivative, on a surface to be investigated for the presence of blood and detecting the presence, or absence, of blood depending on spectral response Further, the present invention relates to chemical compositions for detecting presence, or absence, of blood. Furthermore, the present invention relates to a kit for detecting presence, or absence, of blood comprising the chemical composition. | 04-05-2012 |
20120094389 | Single Nucleotide Polymorphisms That Predict HCV Treatment Outcomes - The present invention is based on the discovery of associations that exist between single nucleotide polymorphisms (SNPs) on chromosome 4 and virological outcomes in a diverse population of patients with hepatitis C virus (HCV) who received interferon-based treatment. | 04-19-2012 |
20120100623 | METHOD OF OLIGONUCLEOTIDE SEQUENCING BY MASS SPECTROMETRY - A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide is provided. In certain embodiments, the method comprises: a) inputting the nucleotide sequence of an oligonucleotide; b) executing an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide; c) comparing the predicted m/z values of the predicted molecular formulas to experimentally-obtained m/z values obtained by analysis of the oligonucleotide by tandem mass spectrometry to determine if the predicted masses correspond with the experimentally-obtained masses. The method may be used, for example, to confirm the identity of a. oligonucleotide after it is synthesized, i.e., to confirm that it has the expected sequence. | 04-26-2012 |
20120100624 | OPTICAL REACTION MEASUREMENT DEVICE AND OPTICAL REACTION MEASUREMENT METHOD - Provided is an optical reaction measurement device that measures whether a reaction is occurring between a sample and a reagent, or how a reaction is progressing, on the basis of changes over time of optical properties of a liquid mixture of the sample and the reagent. The preparation time required for tasks such as mixing the sample and the reagent is predetermined, and when a start switch on the measurement device is turned to “on,” a timer starts running, and the time remaining until the preparation time begins is shown to an operator as a countdown. When the count reaches zero, the operator is informed that the preparation time has begun. The amount of time that has passed since the preparation time began is used as time data to accompany measurement data, and optical property data from after the end of the preparation time is used in analysis. | 04-26-2012 |
20120107942 | COMPOUNDS AND METHODS FOR RAPID LABELING OF N-GLYCANS - The present invention provides compounds and methods for rapid labeling of N-glycans, for example, rapid fluorescent labeling of N-glycans. In one aspect, the present invention provides fluorescent carbamate or thiocarbamate compounds. Upon contacting with N-glycans, the compounds undergo facile reactions with N-glycans to form fluorescent-labeled N-glycans. | 05-03-2012 |
20120107943 | Labelling Strategies for the Sensitive Detection of Analytes - The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological samples. | 05-03-2012 |
20120115238 | NUCLEIC ACID EXTRACTION KIT, NUCLEIC ACID EXTRACTION METHOD, AND NUCLEIC ACID EXTRACTION APPARATUS - A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells | 05-10-2012 |
20120135530 | Detection of target analytes using particles and electrodes - The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined. | 05-31-2012 |
20120164742 | In-Line Derivatization Of Polyaminosaccharide Polymer For Analytical Determination - An analytic method for the determination of the concentration of polysaccharide polymer in solution comprises the in-line derivatization of the polymer by reaction with sulfonate dye. The reaction results in an analyte-derivative, carried in a mobile phase, that can be detected and quantitatively determined by ultraviolet absorption or by fluorescence measurement. The method can provide sensitive and precise measurement in spite of limited solubility of the analyte. | 06-28-2012 |
20120196375 | Compositions and Methods for Detection of Soils on Fabrics - The present invention is in the field of household cleaning, for example laundry products and methods. The invention is directed to the use of indicator materials, such as fluorescent indicator materials, for detecting or visualizing organic laundry soils, particularly those that tend to be invisible to the naked eye, such as sebum, perspiration, biological soils, odor-causing soils/stains and tannins. In addition, the present invention is directed to methods of using indicator materials, such as fluorescent indicator materials, to detect such soils on fabrics, and to determine and demonstrate the cleaning efficacy of a laundry product. | 08-02-2012 |
20120196376 | NANOFILTER DEVICES USING ELASTOMERIC MICRO TO NANOCHANNEL INTERFACES AND METHODS BASED THEREON - A method is provided for fabricating a nanochannel. The method comprises providing a microchannel and controlling collapse of the microchannel so that it collapses to form a nanochannel of desired dimensions. The method employs a collapsible, flexible material such as the elastomer polydimethylsiloxane (PDMS) to form the nanochannel. A master is provided that is configured to have geometric conditions that promote a desired frequency of microchannel collapse. A collapsible material having a stiffness that also promotes a desired frequency of microchannel collapse is molded on the master. The molded collapsible material is removed from the master and bonded to a base, thereby forming the microchannel, which then collapses (or is collapsed) to form the nanochannel of desired dimensions. Nanofluidic and microfluidic devices comprising complex nanochannel structures and micro to nanochannel transitions are also provided. | 08-02-2012 |
20120196377 | FLUORESCENT MOLECULE AND METHOD FOR DETECTING TARGET NUCLEIC ACID - Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I′): | 08-02-2012 |
20120202293 | Highly Multiplexed Particle-Based Assays - Methods are provided for detecting and optionally quantitating multiple analytes, including nucleic acid and/or polypeptide analytes, in particle-based assays that can be highly multiplexed. Compositions, systems, and kits related to the methods are also featured. | 08-09-2012 |
20120208283 | Systems and Methods for Detection and Quantitation of Analytes Using an Oscillating Stimulus - Described systems and methods allow the detection of and determination of a concentration of a target analyte such as a biological cell, a virus, a polypeptide, a toxin, a pesticide, a drug, a drug residue, or a DNA strand, in a fluid sample. A variable stimulus, such as an oscillating magnetic field or a light beam of oscillating intensity, is applied to the sample, inducing variations in a position or shape of a constituent of the sample, or variations in a fluorescence of the sample. Such variations produce measurable variations in electric and/or optical properties of a sensor, variations which allow the determination of the concentration of the target analyte. | 08-16-2012 |
20120208284 | HEPATOCELLULAR CARCINOMA MARKER - A hepatocellular carcinoma marker comprising a sugar chain represented by any one of the (1) to (7) shown below is used for a method for diagnosis of hepatocellular carcinoma, especially for differential diagnosis between liver cirrhosis and hepatocellular carcinoma. | 08-16-2012 |
20120214246 | System and Method for Extending Dynamic Range for a Detector - A system and method for measuring signals having a wide range of intensity components using detectors adapted for use in biological analysis devices. In certain biological analysis applications, signals emitted by a sample may have intensity components that vary over several orders of magnitude. Measurement of such a signal may yield an acceptable quality for one intensity component at the expense of another component. For example, a detector configured to measure a relatively weak intensity component may cause it to overflow when subjected to a relatively strong intensity component. The detector can be adapted to be operated at different configurations to allow measurements of different components of the signal, and the results can be combined to yield an accurate representation of the signal. | 08-23-2012 |
20120244625 | Reductive Amination and Analysis of Carbohydrates Using 2-Picoline Borane as Reducing Agent - The invention provides the use of 2-picoline borane (2-PB) for the reductive amination of carbohydrates, especially glycans from book plasma, wherein the concentration of 2-PB is less than the concentration of NaBH(OAc) | 09-27-2012 |
20120276646 | METHODS AND REAGENTS FOR ANALYZING RIBOSWITCHES USING FRET - The present invention is provides isolated riboswitches with FRET pairs for distinguishing changes in regulatory interactions controlled by the expression platform domain found in riboswitches. The invention further provides methods of using those riboswitches to detect structural changes in the expression platform domain and to identify potential antibiotics. | 11-01-2012 |
20120282702 | Novel Compounds and Synthesis of Tellurium-Derivatized Oligonucleotides for Structural and Functional Studies - Disclosed are compounds of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer. Also disclosed are methods of preparing compound of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer. Further disclosed are methods of conducting drug discovery and research comprises applying the compound of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer in an investigation. | 11-08-2012 |
20120288948 | CONTROLLED TUNNEL GAP DEVICE FOR SEQUENCING POLYMERS - The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo- or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer. | 11-15-2012 |
20130005042 | HYBRID SINGLE MOLECULE IMAGING SORTER - A system for characterizing and sorting individual molecules, for example chromatin or DNA molecules. In some configurations, the system can immobilize molecules of interest suspended in fluid in a sample stage for characterization, and selectively release the immobilized molecules to different output reservoirs, depending on the characterization result. For example, fluorescent markers may be hybridized onto molecules, which may then be attached to dielectric beads and immobilized using holographic optical tweezers or other means. The system may provide elongational flow to elongate molecules, and may detect the presence and spacing of multiple fluorescent markers hybridized to the molecules. Using advanced techniques, the system may be able to characterize features with a resolution better than the theoretical resolution of the system optics. The system may utilize a microfluidic cartridge. | 01-03-2013 |
20130005043 | Detection and Quantitation of Cyclodextrins - The invention relates to the detection and quantitation of cyclodextrins and cyclodextrin derivatives in solutions comprising a protein. The invention further relates to methods of evaluating pharmaceutical preparations for the presence of residual cyclodextrins. | 01-03-2013 |
20130011927 | DETECTION OF IMMOBILIZED NUCLEIC ACID - The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. | 01-10-2013 |
20130011928 | OPTICAL DETECTION SYSTEM AND/OR METHOD - An optical detection system ( | 01-10-2013 |
20130040396 | FLUOROMETER WITH LOW HEAT-GENERTING LIGHT SOURCE - This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present fluorometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The fluorometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each fluorescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled. | 02-14-2013 |
20130071938 | METHOD FOR ANALYZING SUGAR CHAIN BY MASS SPECTROMETRY - It has been found out that it is possible to increase the ionization efficiency of a sample sugar chain by methylating hydroxyl groups of the sugar chain before MALDI-TOF MS measurement. This enables quantitative and structural analyses on the sample sugar chain with high accuracy. | 03-21-2013 |
20130095570 | SUGAR ANALYSIS DEVICE AND ANALYSIS METHOD - Disclosed is a method separation analysis of reducing sugars with enhanced sensitivity and an analytical apparatus therefore employing the post-column fluorescence detection/boric acid complex anion exchange method. The method disclosed is a method for analysis of reducing sugars using the post-column fluorescence detection/boric acid complex anion exchange method, and characterized in that a back-pressure generator is installed in the flow path between the heater, which is for causing a reaction by heating a sample separated by column chromatography with a basic amino acid, and a fluorometric detector. | 04-18-2013 |
20130102083 | METHOD FOR DETERMINING ONE OR MORE CHARACTERIZING FEATURES OF A MACROMOLECULE AND AN APPARATUS FOR CARRYING OUT SAID METHOD - The invention concerns a method and apparatus for determining one or more characterizing features of a macromolecule, in particular torque and/or twist of nucleic acids like DNA, using magnetic fields. | 04-25-2013 |
20130102084 | Methods of forming graphene by graphite exfoliation - Methods of forming graphene by graphite exfoliation, wherein the methods include: providing a graphite sample having atomic layers of carbon; introducing a salt and a solvent into the space between the atomic layers; expanding the space between the atomic layers using organic molecules and ions from the solvent and the salt; and separating the atomic layers using a driving force to form one or more sheets of graphene; the graphene produced by the methods can be used to form solar cells, to perform DNA analysis, and for other electrical, optical and biological applications. | 04-25-2013 |
20130122601 | Method for Detecting Content of Chitosan Fiber in Textile - The technical solution of the present invention provides a method for measuring content of chitosan fiber in textile by using a series of equations. The method calculates the content of chitosan fiber in textile according to the following equations: | 05-16-2013 |
20130149787 | Devices and Methods for Detection of Biomolecular Interactions - Devices, systems, and methods are provided for the detection of biomolecular interactions. The interactions between one or more target DNA strands, one or more receptor DNA strands, and one or more probe DNA strands, if necessary, are used to detect the one or more target DNA strands. The one or more target DNA strands or the one or more probe DNA strands may be coupled to a magnetic bead, and the one or more receptor strands may be coupled to the Hall device. | 06-13-2013 |
20130157376 | Thermal Cycler Calibration Device and Related Methods - Methods, devices, and systems are provided for calibrating heat sources of thermal cyclers. | 06-20-2013 |
20130177992 | EVALUATING HEPARIN PREPARATIONS FOR PHARMACEUTICAL USE - The disclosure features methods of analyzing preparations of heparin, and materials derived from heparin using strong anion exchange high performance liquid chromatography (SAX-HPLC). | 07-11-2013 |
20130183763 | GELATION MEASURING APPARATUS AND SAMPLE CELL - In a measuring apparatus for measuring a target substance in a sample cell via a gelation reaction, a sample cell houses a specimen containing the substance to be measured, and a solution containing a gelating reagent is irradiated with a laser beam. The solution in the sample cell is stirred to generate minute and uniform gel particles, which are caused to pass through the laser beam. Scattered light from the gel particles generated in the sample cell is detected by a photodiode array, and the scattered-light intensity of the generated gel particles or the diameter and the number thereof is measured on time series by a computer on the basis of a scattered-light detection output of the photodiode array. | 07-18-2013 |
20130183764 | HIGH PURITY HEPARIN AND PRODUCTION METHOD THEREFOR - The present invention provides a high purity heparin useful to be a pharmaceutical product, cosmetics, research reagent, or the like, and a method for producing the same, more specifically, a heparin which does not substantially contain a nitrous acid degradation-resistant impurity and a method for producing a heparin, comprising mixing an aqueous solution of 5 to 30% by weight of the heparin with ethanol having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin. | 07-18-2013 |
20130183765 | Ultraviolet and High-Performance Liquid Chromatography Methods for the Evaluation of Sunscreen Efficacy - Disclosed are compositions which can mimic DNA and/or RNA in cells of a subject and methods of using them as a substrate in testing efficacy of one or more compositions in reducing and/or preventing radiation, such as ultraviolet (UV) radiation-caused DNA and/or RNA damage of said subject. Also disclosed are systems related to the disclosed methods. | 07-18-2013 |
20130189790 | SENSOR ARRAYS AND METHODS FOR MAKING SAME - A system includes a sensor including a sensor pad and includes a well wall structure defining a well operatively connected to the sensor pad. The sensor pad is associated with a lower surface of the well. The well wall structure defines an upper surface and a wall surface extending between the upper surface and the lower surface. The upper surface is defined by an upper buffer material having an intrinsic buffer capacity of at least 2×10 | 07-25-2013 |
20130196444 | Method and Device for Detection of an Analyte - The present invention relates to a method, device, and kit for analyzing a sample for determining the presence or amount of an analyte, particularly carbohydrate, more particularly sugar, in the sample using a fabric. | 08-01-2013 |
20130203177 | GEL PARTICLE MEASUREMENT REAGENT AND MEASUREMENT METHOD USING SAME - Provided is a gel particle measurement reagent effective in quickly measuring a time point of initiation of production of gel particles. A gel particle measurement reagent R is a gel particle measurement reagent to be used to be agitated continuously with a sample S containing a target substance St as a measuring object to turn the target substance St into gel particles, including: a reagent base material | 08-08-2013 |
20130252341 | METHOD AND DEVICE FOR DETECTING NUCLEIC ACID, AND KIT - The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method. | 09-26-2013 |
20130273664 | ISFET SWITCH - There is provided a semiconductor device for detecting a change in ion concentration of a sample and method of using same. The device comprises a plurality of Field Effect Transistors (FETs) coupled to a common floating gate and an ion sensing layer exposed to the sample and coupled to the floating gate. There may be other input voltages coupled to the floating gate. | 10-17-2013 |
20130288380 | METHOD FOR DETECTING FORMATION OF G-QUADRUPLEX - It is an object of the method of the present invention to specifically detect a G-quadruplex even in the presence of potassium ions. | 10-31-2013 |
20130302901 | Electrodes for Sensing Chemical Composition - Some embodiments of the present disclosure provide methods, devices, and systems for sequencing nucleic acid polymers that utilize palladium (Pd), for example, at least in part, as an electrode material that is (i) functionalized with one or more adaptor molecules and (ii) capable for use to sense one or more chemical compositions. | 11-14-2013 |
20130309776 | Graphene-Based Nanopore and Nanostructure Devices and Methods for Macromolecular Analysis - Provided are graphene-based nanopore and nanostructure devices, which devices may include an insulating layer disposed atop the graphene, which can be in a planar shape or nanostructured into a ribbon or other shapes, containing a single graphene layer or several layers. Graphene layers and nanostructures can be placed nearby horizontally or stacked vertically. Also provided are related methods of fabricating and processing such devices and also methods of using such devices in macromolecular analysis. | 11-21-2013 |
20130323851 | CARBOXAMIDE-SUBSTITUTED DYES FOR ANALYTICAL APPLICATIONS - The present invention relates to carboxamide-substituted dyes, the production and use of such dyes as labeling groups in analytics. | 12-05-2013 |
20130337569 | SERS Nanotag Assays - Methods and systems for the use of Surface Enhanced Raman Scattering nanotags (SERS nanotags) to create homogeneous (no-wash), heterogeneous or sequence detection assay platforms. In certain embodiments the SERS nanotags are used in combination with magnetic particles. Multiplexed assay platforms are also disclosed. In certain embodiments, the assay is useful for clinical proteomics. Assay platforms suitable for use within a biological matrix, for example within whole blood or serum are also disclosed. The assay formats described herein may be used to detect any analyte of interest including but not limited to the detection of cells, viruses, bacteria, proteins, DNA, RNA, or small molecules in any type of biological (animal or plant kingdom) or environmental samples including but not limited to whole blood or serum, occult samples, urine, feces, air, drinking water, phage, any organism, multicellular clumps of cells, for example, cancer tissue homogenate. | 12-19-2013 |
20130337570 | METHOD FOR QUANTITATIVE ANALYSIS OF SUGARS, SUGAR ALCOHOLS AND RELATED DEHYDRATION PRODUCTS - An improved method is provided for the quantitative analysis of mixtures including various sugars, sugar alcohols and related dehydration products, whereby these are enabled to be effectively and accurately quantitated through gas chromatography, for example, by their derivatization with a carboxylic acid, carboxylic acid anhydride or halide in the presence of a metal triflate catalyst. The method can be carried out at essentially room temperature conditions, with a sufficiently rapid and complete derivatization, even in the presence of substantial amounts of water, that the materials to be quantitated do not substantially break down or degrade and substantially completely accounted for in a derivatized form. | 12-19-2013 |
20140024125 | STUDY OF POLYMER MOLECULES AND CONFORMATIONS WITH A NANOPORE - The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer. | 01-23-2014 |
20140024126 | FLOW-CHANNEL DEVICE FOR DETECTING LIGHT EMISSION - The present invention provides a flow-channel device for detecting light emission, which suppresses a noise originating in unnecessary light emission, and can be simply bonded with the use of an organic material. The flow-channel device having a flow channel is structured by the bonding of at least two substrates, wherein at least any one substrate has a first groove which constitutes the flow channel, and a second groove for arranging an adhesive therein which contains an organic material, and a light-shielding layer is provided on an inner wall of the second groove so as to block a light emitted from the second groove from penetrating into the first groove. | 01-23-2014 |
20140030811 | Gradient Structures Interfacing Microfluidics and Nanofluidics, Methods for Fabrication and Uses Thereof - The present invention relates to a device for interfacing nanofluidic and microfluidic components suitable for use in performing high throughput macromolecular analysis. Diffraction gradient lithography (DGL) is used to form a gradient interface between a microfluidic area and a nanofluidic area. The gradient interface area reduces the local entropic barrier to nanochannels formed in the nanofluidic area. In one embodiment, the gradient interface area is formed of lateral spatial gradient structures for narrowing the cross section of a value from the micron to the nanometer length scale. In another embodiment, the gradient interface area is formed of a vertical sloped gradient structure. Additionally, the gradient structure can provide both a lateral and vertical gradient. | 01-30-2014 |
20140038301 | SELECTIVE CAPTURE AND RELEASE OF ANALYTES - The described subject matter includes techniques and components for minimally invasive, selective capture and release of analytes. An aptamer is selected for its binding affinity with a particular analyte(s). The aptamer is functionalized on a solid phase, for example, microbeads, polymer monolith, microfabricated solid phase, etc. The analyte is allowed to bind to the aptamer, for example, in a microchamber. Once the analyte has been bound, a temperature control sets the temperature to an appropriate temperature at which the captured analyte is released. | 02-06-2014 |
20140045270 | DEVICE HAVING NANOPORE WITH THIOL-CONTAINING MATERIAL ATTACHED TO GOLD LAYER AND METHOD OF ANALYZING NUCLEIC ACID USING THE DEVICE - Provided is a device with a nanopore that has a thiol-containing material bound to a gold layer, methods of producing the devices, and methods of analyzing nucleic acid using the devices. | 02-13-2014 |
20140051175 | INSTRUMENT AND METHOD FOR ANALYSIS OF MANNOSE 6-PHOSPHATE - Disclosed are an apparatus and method for separation analysis of mannose-6-phosphate (M6P) by post-column fluorescence detection method. The apparatus is based on chromatography, and includes a column with a solid phase having affinity for phosphate, a flow path for the eluate, a heater installed on the flow path for M6P and a basic amino acid to react by heating the eluate in the flow path, and a fluorescence detector installed downstream of the heater for continuously irradiating the eluate with excitation light and measuring the intensity of the emission, and may include in the flow path a supply channel for addition of a basic amino acid between the column and the heater. The method is characterized in that it uses the apparatus and a second mobile phase consisting of a second buffer containing phosphate of predetermined concentration and adjusted to a predetermined pH. | 02-20-2014 |
20140057357 | METHOD FOR QUANTITATIVELY DETECTING 8-OXO 2'-DEOXYGUANOSINE IN AQUEOUS SAMPLE SOLUTION WITH HIGH SENSITIVITY - There is provided a method for quantitatively detecting 8-oxo 2′-deoxyguanosine in an aqueous sample solution with high sensitivity. A method for quantitatively detecting 8-oxo 2′-deoxyguanosine in an aqueous sample solution, including the steps of 1) immobilizing a fluorescent probe molecule showing a fluorescence response specific to 8-oxo 2′-deoxyguanosine on surfaces of fine particles via a spacer unit and bringing the sample solution into contact with the fine particles, and 2) measuring a physical property of the fine particles before and after the contact with the sample solution to determine a change in the physical property. | 02-27-2014 |
20140057358 | ANALYSIS OF SULFATED POLYSACCHARIDES - The invention relates to methods and products associated with analyzing and monitoring heterogeneous populations of sulfated polysaccharides. In particular therapeutic heparin products including low molecular weight heparin products and methods of analyzing and monitoring these products are described. | 02-27-2014 |
20140073058 | Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology - Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. | 03-13-2014 |
20140073059 | Azide Substituted Naphthylene or Rylene Imide Derivatives and their Use as Reagents in Click-Reactions - Novel mono-azide substituted rylene-imide derivatives, their use in methods for the detection of analytes and reagents kits for the detection of analytes comprising said novel mono-azide substituted rylene-imide derivatives. | 03-13-2014 |
20140080220 | Azide Substituted Naphthylene or Rylene Imide Derivatives and their Use as Reagents in Click-Reactions - Novel mono-azide substituted rylene-imide derivatives, their use in methods for the detection of analytes and reagents kits for the detection of analytes comprising said novel mono-azide substituted rylene-imide derivatives. | 03-20-2014 |
20140087474 | APPARATUS AND METHODS FOR PERFORMING OPTICAL NANOPORE DETECTION OR SEQUENCING - Methods and systems for detecting or sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. Optical sequencing of a molecule may be performed utilizing an optical or photon detector operated in time delayed integration mode. In certain variations, the translocation rate of molecules through a, pore, nanopore or channel may be controlled or reduced by increasing the diameter of the molecules to allow for molecule detection or sequencing by optical or electrical detection. In certain variations, a plurality or an array of, pores, nanopores, or channels may be utilized to optically detect a plurality of molecules. | 03-27-2014 |
20140099726 | DEVICE FOR CHARACTERIZING POLYMERS - Provided is a device comprising (a) a plurality of chambers, each chamber in communication with an adjacent chamber through at least one pore wherein the device contains at least two pores defined as a first and a second pores, (b) means to move at least a portion of the polymer out of the first pore and into the second pore and (c) at least one sensor capable of identifying individual components of the polymer during movement of the polymer through the first and second pores, provided that when only a single sensor is employed, the single sensor does not include two electrodes placed at both ends of a pore to measure an ionic current across the pore. Methods of using the device are also provided. | 04-10-2014 |
20140106462 | MICROFLUIDIC SYSTEM FOR HIGH-THROUGHPUT, DROPLET-BASED SINGLE MOLECULE ANALYSIS WITH LOW REAGENT CONSUMPTION - A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region. | 04-17-2014 |
20140141521 | GRAPHENE TRANSISTOR GATED BY CHARGES THROUGH A NANOPORE FOR BIO-MOLECULAR SENSING AND DNA SEQUENCING - A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore. | 05-22-2014 |
20140170759 | SYSTEM AND METHOD FOR DETERMINING AMINO ACID SEQUENCE OF POLYPEPTIDE - This invention discloses systems and methods for determining the sequence of amino acids in a short peptide chain that constructs a protein. The protein is firstly hydrolyzed to various short peptides and amino acid enantiomers. Then, the systems and method are used to separate the short peptides and the amino acid enantiomers, identify qualitatively each of the amino acid enantiomers, and obtain the molecular mass signal for each of the peptides. After that, the identified amino acid enantiomers are used to construct any possible short peptides in an order from the smallest molecular weight dipeptide to higher molecular weight short peptides, and the correct short peptides is confirmed by matching the molecular weight obtained from the mass spectrometry measurement, then, the short peptides are combined to give a large peptide. The process is continued until the whole amino acid sequence of the peptide chain of protein can be determined. | 06-19-2014 |
20140170760 | OPTICAL ANAYLSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided an optical analysis technique of detecting light of a light-emitting particle in a sample solution in the scanning molecule counting method using the light measurement with a confocal or multiphoton microscope, for suppressing the scattering in detected results of signals of light of light-emitting particles smaller and achieving the improvement of accuracy. The inventive technique comprises moving the position of a light detection region along a predetermined route for multiple circulation times by changing the optical path of the optical system; detecting light from the light detection region and generating time series light intensity data during the moving of the light detection region and detecting individually a signal indicating light from each light-emitting particle existing in the predetermined route using the time series light intensity data obtained in the circulating movements of the light detection region of multiple times. | 06-19-2014 |
20140186966 | SINGLE PHOTON SPECTROMETER - A fiberized single photon sensitive spectrometer on a 32-channel PMT sensor is highly sensitive with broad detection dynamic range. The spectrometer enables accurate and high speed detection, identification and analysis of biological samples labeled with multiple fluorescent markers, such as compositions of multi-color fluorescence signals or radiation emitted by multiple fluorescence dyes. A fiberized optical input of the spectrometer allows an easy and efficient coupling to any measurement system based on fiber collection of the analyzed fluorescence. The spectrometer provides highly accurate DNA sequencing. A 32 channel PMT single photon detector has a detection dynamic range of more than 20 bits and has a frame rate of about 3300 frames per second. The dynamic range of the detector's pixels reaches 10 | 07-03-2014 |
20140186967 | SINGLE PHOTON SPECTROMETER - A fiberized single photon sensitive spectrometer on a 32-channel PMT sensor is highly sensitive with broad detection dynamic range. The spectrometer enables accurate and high speed detection, identification and analysis of biological samples labeled with multiple fluorescent markers, such as compositions of multi-color fluorescence signals or radiation emitted by multiple fluorescence dyes. A fiberized optical input of the spectrometer allows an easy and efficient coupling to any measurement system based on fiber collection of the analyzed fluorescence. The spectrometer provides highly accurate DNA sequencing. A 32 channel PMT single photon detector has a detection dynamic range of more than 20 bits and has a frame rate of about 3300 frames per second. The dynamic range of the detector's pixels reaches 10 | 07-03-2014 |
20140206093 | OPTICAL MEASURING APPARATUS AND METHOD FOR THE ANALYSIS OF SAMPLES CONTAINED IN LIQUID DROPS - An optical measuring apparatus and method for analysis of samples contained in liquid drops provided by a liquid handling system has a liquid handling tip. A light source irradiates the liquid drop; a detector measures sample light; and an optics system with first optical elements transmits irradiation light, and a processor processes the measurement signals. The liquid drop is suspended at the liquid handling orifice of the liquid handling tip in a position where the liquid drop is penetrated by a first optical axis defined by the light source and the first optical elements. The liquid drop is physically touched only by the liquid handling tip and the liquid sample inside the liquid handling tip. A mutual adaption of the size and position of the liquid drop with respect to the first optical elements is achieved. | 07-24-2014 |
20140206094 | MALDI MASS SPECTROMETRY METHOD - The problem is to provide a MALDI mass spectrometry method that can be used for a mass spectrometry method by a general MALDI method with which it is possible to measure multiple molecules to be measured that are contained in a sample quantitatively in a short period of time with good efficiency compared with conventional methods. The problem is solved by a MALDI mass spectrometry method for a sample containing multiple molecules to be measured, which is a MALDI mass spectrometry method characterized in that the multiple molecules to be measured are a saccharide mixture, a glycopeptide mixture, a glycopeptide-peptide mixture, a glycoprotein mixture, or a glycoprotein-protein mixture, and that a quantitative mass spectrum of the multiple molecules to be measured is obtained from an arbitrary point in the sample to be measured where a signal is detected without measuring and integrated averaging the entire sample to be measured. | 07-24-2014 |
20140212981 | METHODS AND SYSTEMS FOR DETERMINING THE AMOUNT OF THIOPURINE METABOLITES IN A SAMPLE - Disclosed are methods and systems for the analysis of thiopurine drug metabolites in a sample using liquid chromatography/mass spectrometry. | 07-31-2014 |
20140220696 | METHODS AND COMPOSITIONS FOR DIRECT DETECTION OF DNA DAMAGE - The present invention is a method for detecting the extent of DNA damage in a subject suspected of having DNA damage wherein the damage results in the formation of aldehyde moieties in DNA comprising, obtaining a DNA sample from the subject, combining the DNA sample with a fluorescent, chromogenic, pro-fluorescent or pro-chromogenic hydrazine compound to from a fluorescent DNA, detecting the presence of the fluorescent DNA by monitoring the fluorescent emission and quantitating the fluorescent emission thereby determining the extent of DNA damage in the subject. | 08-07-2014 |
20140220697 | CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE - A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface. | 08-07-2014 |
20140234980 | DEVICES WITH A FLUID TRANSPORT NANOCHANNEL INTERSECTED BY A FLUID SENSING NANOCHANNEL AND RELATED METHODS - Devices, such as chips for DNA analysis, have at least one fluid transport nanochannel with at least one intersecting (e.g., transverse) sensing nanochannel that can be monitored for change in ionic current to determine characteristics or parameters of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like. | 08-21-2014 |
20140234981 | DOUBLE GATE ION SENSITIVE FIELD EFFECT TRANSISTOR - Devices that include a substrate; a source region and a drain region formed within the substrate and having a channel region provided therebetween; a first insulating layer formed over the channel region; a first floating gate formed over the first insulating layer, the first floating gate configured to respond to an analyte in a target material; and a second gate formed over the first floating gate, the second gate capacatively coupled but not electrically connected to the first floating gate. | 08-21-2014 |
20140242709 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 08-28-2014 |
20140248705 | NUCLEIC ACID LADDERS - The present invention provides nucleic acid compositions or ladders which may be used as standards for estimating the size (in base pairs) and or mass of nucleic acid molecules of unknown size and/or mass. The invention also relates to methods for producing such compositions or ladders, ladders or compositions produced by such methods, and to methods for estimating the size and/or mass of nucleic acid molecules by comparison to these nucleic acid sizing ladders. | 09-04-2014 |
20140273254 | METHOD, PRODUCT, AND SYSTEM FOR QUANTIFYING THE METHYLATION STATUS OF A NUCLEIC ACID IN A SAMPLE - Methods and systems are described for quantifying the methylation status of nucleic acids in a sample utilizing standardized curves derived from methylation-sensitive HRM data. The standardized curves are generated from HRM curves from a plurality of samples, each sample having a known but different methylation status. In one embodiment, the first negative derivative of each HRM curve from the known samples is plotted and a first value corresponding with a first melt peak and a second value corresponding with a second melt peak from the negative derivative plots are identified. The slope of a line connecting the first and second values for each sample is calculated and used to identify a slope data point that is plotted to generate the standardized curve. In another embodiment, a threshold line that intersects the plurality of HRM curves is generated and the standardized curve is generated from the intersection data points. | 09-18-2014 |
20140273255 | RARE EARTH SPATIAL/SPECTRAL BARCODES FOR MULTIPLEXED BIOCHEMICAL TESTING - Hydrogel microparticles spatially and spectrally encoded using upconverting phosphor nanoparticles are described for use in biochemical testing. In each microparticle, upconversion nanocrystals having spectrally distinguishable emission spectra are disposed in different pardons of an encoding region of the microparticle. | 09-18-2014 |
20140295567 | METHOD FOR DETECTING NUCLEIC ACID USING INTERCALATOR-CONJUGATED METAL NANOPARTICLES - The present invention relates to a method for detecting nucleic acid using intercalator-conjugated metal nanoparticles. More particularly, the present invention relates to a method which involves applying a sample containing a target nucleic acid to a DNA chip having a substrate with a DNA probe fixed thereon, reacting metal nanoparticles in which intercalators coupled with a double helix nucleic acid are conjugated, and reacting a metal enhancing solution to thereby amplify the sizes of the metal nanoparticles and detect the target nucleic acid using the unaided eye. The method for detecting nucleic acid according to the present invention uses intercalator-conjugated metal nanoparticles, and thus enables detection of nucleic acid using the unaided eye without any other equipment. Therefore, compared with conventional detection methods, the present invention exhibits the effects of reducing analysis costs and the time needed for detection, and enables miniaturization of the size of an apparatus, thereby enabling field diagnosis in a livestock farm, home, or the like. | 10-02-2014 |
20140315319 | BIOMOLECULE INTERACTION USING ATOMIC FORCE MICROSCOPE - The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized. | 10-23-2014 |
20140356969 | METHOD FOR DETECTING A TARGET PARTICLE - Provided is a method for detecting a target particle that is a method for detecting a non-luminescent target particle dispersed and randomly moving in a sample solution using an optical system of a confocal microscope or multi-photon microscope, having: (a) preparing a sample solution containing target particles, and labeling particles of which the average outer diameter is less than 15% of the diameter of a photodetection region of the optical system, binding two or more molecules of the labeling particles per molecule of the target particles in the sample solution, and forming a non-luminescent complex of which the outer diameter is 15% or more of the diameter of the photodetection region; and, (b) calculating the number of molecules of the complex in the sample solution prepared in the (a) using an inverse scanning molecule counting method. | 12-04-2014 |
20140370612 | Method and System Implementing Spatially Modulated Excitation or Emission for Particle Characterization with Enhanced Sensitivity - A method and system for using spatially modulated excitation/emission and relative movement between a particle (cell, molecule, aerosol, . . . ) and an excitation/emission pattern are provided. In at least one form, an interference pattern of the excitation light with submicron periodicity perpendicular to the particle flow is used. As the particle moves along the pattern, emission is modulated according to the speed of the particle and the periodicity of the stripe pattern. A single detector, which records the emission over a couple of stripes, can be used. The signal is recorded with a fast detector read-out in order to capture the “blinking” of the particles while they are moving through the excitation pattern. This concept enables light detection with high signal-to-noise ratio and high spatial resolution without the need of expensive and bulky optics. | 12-18-2014 |
20140377875 | FLUORESCENT PI MARKERS FOR ISOELECTRIC FOCUSING SEPARATIONS AND FLUORESCENT LABELING - UV-Absorbing and fluorescent pl markers for isoelectric focusing separations and fluorescent labeling, and methods for making and using the markers. | 12-25-2014 |
20150017734 | QUALITY CONTROL MARKER AND ITS USE IN HERBS AUTHENTICATION - The present invention relates to a quality control marker and method of using such marker in qualitative and quantitative authentication of | 01-15-2015 |
20150024504 | Methods and Compositions for Detection and Analysis of Polynucleotides Using Light Harvesting Multichromophores - Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided. | 01-22-2015 |
20150093836 | METHOD FOR CONTROLLING DISSOCIATION OF DOUBLE STRANDED NUCLEIC ACID, METHOD FOR CONTROLLING STRAND EXCHANGE REACTION OF DOUBLE STRANDED NUCLEIC ACID AND METHOD FOR AMPLIFYING NUCLEIC ACID - The present invention relates to a method for controlling dissociation of a double stranded nucleic acid. The present invention also relates to a method for controlling strand exchange reaction of a double stranded nucleic acid. The present invention further relates to a method for amplifying a nucleic acid. | 04-02-2015 |
20150140674 | Method for Determining Weight Concentration of A Polymer Penetrated into A Porous Medium - A polymer solution is dried until full evaporation of water. The polymer formed after drying the polymer solution is heated up and active degradation temperature range at a given heating rate and a degree of polymer degradation within this temperature range are determined. Then the solution is dried and a thermal analysis is conducted within the temperature range including the active degradation temperature range. A loss of mass of a portion of a sample of a porous medium is calculated and a loss of mass of similar sample of the porous medium sample after pumping the polymer solution injection is also determined. Based on the derived values, weight concentration of polymer penetrated into the porous medium is determined. | 05-21-2015 |
20150293111 | METHOD OF DIAGNOSING GALACTOSEMIA IN NEONATAL SCREENING - A method of diagnosing galactosemia in blood samples from neonates by determining GAL-1-P concentrations before 5-7 days of life is disclosed. The removal of interfering compounds allows a more specific and therefore more accurate determination of GAL-IP levels in newborn screening for galactosermia using mass spectrometry. This is of major importance when investigating samples from children that have not yet achieved a steady state of GAL-IP (i.e. before 5-7 day of life). | 10-15-2015 |
20150329600 | MODIFIED ALPHA HEMOLYSIN POLYPEPTIDES AND METHODS OF USE - Provided herein are alpha hemolysin polypeptides comprising modified amino acid sequences that can reduce the rate of translocation of a polymer. Also provided herein are apparatuses and devices comprising modified hemolysin polypeptides. Also provided herein are methods of using modified alpha hemolysin proteins for use in characterizing and/or sequencing a polymer or for use as molecular sensors. | 11-19-2015 |
20150338333 | METHOD FOR MEASURING OPTICALLY TRANSPARENT PARTICLES AND DEVICE FOR MEASURING OPTICALLY TRANSPARENT PARTICLES - The present invention is one that, without performing a complicated measuring process, makes it possible to continuously measure optically transparent particles including a biologically-derived polysaccharide having a negatively-charged functional group, such as transparent exopolymer particles (TEP), and includes: a dyeing step of adding to a sample solution a dye that binds to the negatively-charged functional group of the optically transparent particles to dye the optically transparent particles; an aggregation step of reducing the ionic strength of the sample solution to aggregate the optically transparent particles; and a turbidity measuring step of irradiating inspection light to the dyed and aggregated optically transparent particles, and detecting transmitted light caused by the optically transparent particles to measure the turbidity of the sample solution. | 11-26-2015 |
20160010145 | DETECTION OF RNA WITH SUBSTITUTED UNSYMMETRICAL CYANINE DYES | 01-14-2016 |
20160017313 | ANALYSIS OF MRNA HETEROGENEITY AND STABILITY - Reversed phase-High Performance (High Pressure) Liquid Chromatography (RP-HPLC) and Size Exclusion Chromatography (SEC) methods have been developed for monitoring structural and size heterogeneity as well as stability of large RNA transcripts, including lengths of up to at least 10,000 nucleotides. The methods are designed for significantly larger mRNAs that could be monitored in the past, including lengths of up to at least 10,000 nucleotides, and including chemically modified RNA transcripts. SEC techniques are also used in the preparative purification of large RNA transcripts to remove impurities, including hybridized nucleic acid impurities and multimeric RNA species. All of these techniques are also beneficial in that they can be used for large scale manufacturing of therapeutics. | 01-21-2016 |
20160091495 | VISCOSITY-SENSITIVE DYES AND METHOD - Disclosed viscosity-sensitive reporter compounds and methods allow tracing the changes in viscosity in a biological sample/environment (e.g., in medical, biochemical, biological, and/or pharmaceutical applications). Compounds and methods of the present disclosure can be used to identify the localization and the rheological state of hydrogels, their degradation products and other biostructural materials, including surgical implants, as well as the effectiveness of drug delivery by hydrogel carriers. | 03-31-2016 |
20160115471 | ENRICHMENT OF SMALL NUCLEIC ACIDS - Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA. | 04-28-2016 |
20160116379 | APPARATUSES FOR STERILELY DELIVERING FLUID - Provided herein are apparatuses, systems, kits and methods for sterilely delivering fluid to an elongated swab. In particular, provided herein are apparatuses configured to sterilely deliver a desired fluid to a swab contained within a swab housing. | 04-28-2016 |
20160139050 | METHOD AND SYSTEM FOR IMAGING A MOLECULAR STRAND - The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X | 05-19-2016 |
20160139136 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 05-19-2016 |
20160168196 | COMPOUND FOR LABELING SUGAR CHAIN SAMPLE | 06-16-2016 |
20160178488 | MICROFLUIDIC TRANSDUCER | 06-23-2016 |