Inventors list

Assignees list

Classification tree browser

Top 100 Inventors

Top 100 Assignees


INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS

Subclass of:

436 - Chemistry: analytical and immunological testing

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
436524000 Carrier is inorganic 59
436528000 Carrier is organic 42
436523000 Carrier is particulate and the particles are of intentionally different sizes or impregnated differently with the immunochemicals 6
436519000 Carrier is a biological cell or cell fragment 1
20100099203Substrates with surfaces modified with PEG - Compositions and methods of modifying surfaces using hydroxyl terminated PEG are described herein. The surfaces so modified are useful in detection of synthetic and natural product organic molecules, organometallics, biomolecules, particles, or cells.04-22-2010
Entries
DocumentTitleDate
20110207238BIOLOGICAL SUBSTANCE ANALYZING METHOD, AND BIOLOGICAL SUBSTANCE ANALYZING CELL, BIOLOGICAL SUBSTANCE ANALYZING CHIP, AND BIOLOGICAL SUBSTANCE ANALYZING APPARATUS EMPLOYED IN THE BIOLOGICAL SUBSTANCE ANALYZING METHOD - Detection of detection target substances at a sensor portion is expedited and the efficiency thereof is improved in biological substance analysis, to enable accelerated analysis with high sensitivity. A biological substance analyzing cell equipped with a reaction chamber having a sample supply space, an acoustic matching layer which is provided at a predetermined region of an inner wall of the reaction chamber that faces another inner wall, and a sensor portion provided within the reaction chamber is employed. Ultrasonic waves are emitted such that a standing wave are generated between the acoustic matching layer and the inner wall of the reaction chamber that faces the acoustic matching layer. The detection target substance is concentrated by the capturing forces of the standing waves, and the concentrated detection target substance is detected at the sensor portion.08-25-2011
20090203151IMMUNOASSAY METHOD - It is an object of the present invention to provide an immunoassay method which is capable of simultaneous quantification of a plurality of test substances under a same analysis condition, through adjustment of the measurement sensitivity/range by simply varying the size of particles for use as labels, without changing the spectra of these particles. The present invention provides an immunoassay method which comprises: simultaneously or step-by step developing respective of the plurality of the test substances and respective of labeling substance particles labeled with first binding substance against the respective of the plurality of the test substances on an insoluble carrier; capturing the test substances and the labeling substance particles in reaction portions on the insoluble carrier that has been immobilized with the respective of second binding substances against the respective of the plurality of the test substances at different positions; and measuring optical characteristic of the labeling substance particles, so as to simultaneously detect the plurality of the test substances, wherein the plurality of the test substances having different detection concentration ranges are simultaneously detected with use of the labeling substance particles which are different in the particle size corresponding to the test substances.08-13-2009
20100112725METHOD TO INCREASE SPECIFICITY AND/OR ACCURACY OF LATERAL FLOW IMMUNOASSAYS - The present invention includes methods and devices for preventing interfering substances from affecting the accuracy of a lateral flow immunoassay. In preferred embodiments, a test strip includes a capturing zone that includes at least one mobile capturing reagent that separates at least one interfering substance from the analyte. The capturing zone is preferably located upstream of the sample application zone. In some embodiments, the reagent/conjugate zone is also located upstream of the sample application zone. The capturing zone may be located upstream, downstream, or overlapping with the reagent/conjugate zone in these embodiments. In other preferred embodiments, one or more mobile capturing reagents are included in the elution medium/running buffer. In yet other embodiments, the capturing reagent is incorporated into a sample collection device of a sample collection system, preferably separate from the chromatographic test strip. A lysis zone is also included in some preferred embodiments.05-06-2010
20100075440ASSAY METHOD - A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.03-25-2010
20100075439ULTRA-SENSITIVE DETECTION OF MOLECULES BY CAPTURE-AND-RELEASE USING REDUCING AGENTS FOLLOWED BY QUANTIFICATION - The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.03-25-2010
20100041167DRUG FOR DIAGNOSING LARGE INTESTINAL CANCER AND/OR POLYP, OBSERVING POSTOPERATIVE COURSE AND MONITORING RECURRENCE - The present invention is directed to a method for diagnosing large intestinal cancer and/or polyp and a method for observing postoperative course or monitoring recurrence thereof, wherein each method includes detecting cystatin SN protein by use of an anti-cystatin SN antibody. The present invention is able to provide a kit for assaying cystatin SN, which can be used, in a simple manner, in a diagnosis performed prior to conventional barium enema examination and endoscopic examination which impose burdens on patients; as an indicator of metastasis and recurrence; and in the evaluation of therapeutic effects. The present invention provides a method for diagnosing or monitoring large intestinal cancer and/or polyp which can be performed in a simple manner, and thus can allow to design a new regimen rapidly.02-18-2010
20090130777METHOD FOR EVALUATING ANALYTE - In an analyte evaluation method for evaluating an analyte, AC voltage is applied between a substrate electrode on a substrate and a counter electrode, and signals obtained from a marker provided on an analyte bound to the substrate electrode are observed, wherein the frequency of the AC voltage is changed and the behavior of the average value of the marker signals is observed. A novel, highly-selective, low-noise method of evaluating a object of evaluation is thus achieved.05-21-2009
20100330701USE OF HEAT-RESISTANT BIOTIN-BINDING PROTEIN, AND SOLID SUPPORT HAVING THE PROTEIN ATTACHED THERETO - The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.12-30-2010
20100068826HYBRID PHASE LATERAL FLOW ASSAY - The invention relates to devices for performing single step assays for the determination of the presence or absence of an analyte in a liquid sample, and methods of determining the presence or absence of such analytes using such devices. Devices disclosed comprise a labeled analyte-binding reagent reversibly-immobilized on a non-porous solid material, which solid material is in physical contact with a dry porous carrier bearing an immobilized analyte-binding reagent. Also provided are quantitative assay devices.03-18-2010
20090124024OPTICAL-WAVEGUIDE SENSOR CHIP, METHOD OF MANUFACTURING THE SAME, METHOD OF MEASURING SUBSTANCE, SUBSTANCE-MEASURING KIT AND OPTICAL-WAVEGUIDE SENSOR - An optical-waveguide sensor chip includes an optical waveguide having a first substance immobilized on the surface thereof, the first substance being specifically reactive with an analyte substance, and fine particles dispersed on the optical waveguide and having a second substance immobilized on the surface thereof, the second substance being specifically reactive with the analyte substance.05-14-2009
20090298199IMMUNOSORBENT ASSAY SUPPORT AND METHOD OF USE - Embodiments of the present invention provide an immunosorbent assay support immobilized with an intermediate binding antibody and their method of use in an improved immunoassay format.12-03-2009
20120225497ZWITTERION-CONTAINING ACRIDINIUM COMPOUNDS - Hydrophilic, chemiluminescent acridinium compounds containing zwitterions are disclosed. These acridinium compounds, when used as chemiluminescent labels in immunochemistry assays and the like, exhibit decreased non-specific binding to solid phases and provide increased assay sensitivity.09-06-2012
20100124789ELECTRONIC DETECTION OF INTERACTION AND DETECTION OF INTERACTION BASED ON THE INTERRUPTION FLOW - Porous members can be positioned so as to partially or fully span channels in microfluidic systems. The porous members can be assembled and/or disassembled in situ. The porous members can be made such that pores are separated by connections including but a single molecule at one location, allowing for a high level of open area in a very small pore size member. The porous member can be made up of colloid particles interconnected with molecular species. These can be used to detect analytes qualitatively and/or quantitatively, or to selectively bind and/or release agents on command for a variety of purposes including first blocking, then opening a channel, concentrating analyte over time followed by release of analyte and detection downstream, etc. Porous members can define valves in multiple-channel systems and, with controlled binding and release of agents at the porous members, these valves can be opened and closed and fluid flow controlled in a multi-channel system. Fluidic systems of the invention can include multiple sensing locations at which different analytes are determined. Systems of the invention provide flexibility for overall microchemical analysis, sequentially, of a variety of agents.05-20-2010
20090047746Analytical Method and Analytical Apparatus - [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.02-19-2009
20100087012Sample Collector and Processor - An aerosol sample collector with an air flow path comprising: (i) at a first segment thereof, a particle charging device, and (i) at a second segment thereof, deflection plates configured to focus particles of a desired charge into a preselected cross-section of the air flow path. The air flow path also includes charged substrates arranged at an outflow portion of the air flow path to collect charged particles on a collection surface of the charged substrates; and an exit path for flowing particles not in the preselected cross-section of the air flow path away from the charged substrates. Related methods are also provided.04-08-2010
20080318340Method of Detecting Target Substances - The present invention provides a method for identifying and detecting multiple types of target substances contained in a specimen, comprising the steps of: contacting the target substances immobilized and/or nonimmobilized on a support with labeled binders capable of identifying corresponding types of target substances so as to form complexes between the target substances and the binders; and detecting the labels in the binders forming the complexes.12-25-2008
20120238039NOVEL IMMOBILIZING FUSION PROTEIN FOR EFFECTIVE AND ORIENTED IMMOBILIZATION OF ANTIBODY ON SURFACES - The present invention relates to a novel fusion protein comprising Staphylococcal protein A and mussel adhesive protein, a biochip comprising a solid substrate to which the fusion protein is attached, and a method for detecting a target antigen in a biological sample using the biochip. Furthermore, the present invention relates to a polynucleotide encoding the fusion protein, a recombinant vector comprising the polynucleotide, a transformed cell comprising the recombinant vector, and a method of preparing the fusion protein by transformed cell comprising the recombinant vector.09-20-2012
20100062544Saturation Assay - The invention provides a modified form of saturation assay, which is based on the measurement of a free or unbound labelled reagent fraction (such that there is an increase in signal in the presence of analyte) and employs trapping zones to concentrate said unbound or free labelled fraction to avoid loss of sensitivity. Preferably, the assay is a membrane assay.03-11-2010
20100297779Biochip Self-Calibration Process - The invention relates to a calibration process for determining the presence and/or the amount of a target compound in a test sample, using a solid support, attached to the surface of which are a compound capable of binding specifically with said target compound and a calibration probe compound, the molar ratio of said compound Csc to said compound Cs being known, in particular for nucleic acid or protein biochips. The invention also comprises the use of such a support for the self-calibration of a measurement, in particular by surface plasmon resonance or by fluorescence, and also a device or a kit comprising such a support and a standard calibration reagent.11-25-2010
20100015726SINGLE COLUMN IMMUNOLOGICAL TEST ELEMENTS - A plurality of individual single column test elements are provided for use in a clinical testing apparatus. Each test element is defined by a single test column that includes a quantity of a test material, such as gel material or a bead matrix, including a cover strip used to access the contents of the test column. Individual test elements can be stored, retained and dispensed for testing patient samples.01-21-2010
20090170220BISPECIFIC CAPTURING MOLECULE - A capturing molecule having an association containing a plurality of polypeptide chains that specifically bind to different sites of a target substance, characterized in that each of the polypeptide chains has a domain having a hypervariable loop structure at a binding site binding to the target substance and an association portion for forming the association, and the polypeptide chains are associated via the association portions present in the polypeptide chains.07-02-2009
20090311804MAGNETIC BEAD ASSISTED SAMPLE CONDITIONING SYSTEM - A magnetic bead assisted sampling system for a fluid sensor. Magnetic beads are dispersed in a sampling volume for collecting the analyte. The beads are packed into a small volume for pre-concentration of the analyte. A solvent may be applied to the beads to elute the analyte from the beads for movement to an analyzer.12-17-2009
20090148961SMART HYDROGEL PARTICLES FOR BIOMARKER HARVESTING - Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles may be changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.06-11-2009
20090148960Method for analysis of compound-binding ability of protein - The present invention provides a method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding a certain amount of the one fraction obtained in step (b), or a certain amount of a mixture of all the fractions or a mixture of plural contiguous fractions among the fractions obtained in step (b), to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.06-11-2009
20100267169ELECTROMAGNETIC MOLECULAR SENSORS AND METHODS OF USING SAME - Devices having an electromagnetic detector for the detection of analytes are disclosed. The devices include an electromagnetic detector, including effective inductance-change magnetic detectors, and a binding moiety. The device can include an electromagnetic material that can be detected by the detector. The device is configured such that binding of an analyte to the binding moiety changes the relationship between the electromagnetic detector and the electromagnetic material such that a change in electromagnetic field is detected by the electromagnetic detector.10-21-2010
20110136262INTEGRATED MICROCHIP SENSOR SYSTEM FOR DETECTION OF INFECTIOUS AGENTS - An integrated multiplexed acoustic wave biosensor chip system with enhanced sensitivity has been developed. The biosensor system incorporates one or more microfluidic channels, coated with target-specific binding films enabling rapid and early detection of viral, bacterial or parasitic targets such as Dengue virus and sexually transmitted diseases in specimens from potentially infected patients. The biosensors are used in portable analytical systems that are suitable for real-time point of care (POC) clinical diagnosis in cost sensitive and/or resource limited settings. The highly sensitive biosensors utilize thinned single crystal piezoelectric substrates that propagate layer guided shear horizontal acoustic plate mode (LG-SH-APM) waves in sensing regions bearing immobilized binders that provide simultaneous and direct detection of mass changes due to multiple bound target pathogens or molecules.06-09-2011
20090087925DEVICES AND METHODS FOR ANALYSIS OF SAMPLES WITH DEPLETION OF ANALYTE CONTENT - A system and method for determining the presence and/or concentration of one or more analytes in a sample that comprises a fluid, the system comprising a solid substrate comprising a sample inlet or inlets and one or more analyte determination flow paths, each analyte determination flow path comprising a defined beginning and a defined terminus and comprising at least one capture zone containing a capture agent for an analyte, the capture agent or agents being immobilized along a portion of the flow path or paths, the flow path or paths being designed so that the one or more analytes are depleted from the sample and bound in a non-linear manner to the portion of the flow path or paths containing immobilized capture agent or agents, producing an analyte depletion end region for each analyte between the beginning and the terminus of the analyte determination flow path.04-02-2009
20090087924MICROFLUIDIC REVERSE AFFINITY-BLOT DEVICE - The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or enrichment of protein(s) from a sample, followed by separation/identification thereof. In general terms, a microfluidic reverse affinity-blot device is a closed system of interconnected components that is comprised of defined points of entry and exit, wherein the interconnected components include a capture region upstream from a protein separation region and subsequent detection region. Methods of use are also described.04-02-2009
20100081215COATING FOR MICROCARRIERS - The present invention relates to carriers, which are coated by at least one layer of polyelectrolytes and one layer of magnetic material. These carriers can be manipulated in a magnetic field. The application of the coating of the present invention on microcarriers comprising a fluorescent core results in a carrier with a homogeneous luminescence. Additionally, where the core is provided with a code, this allows improved reading thereof.04-01-2010
20090263917Method for the quality control of molecules or targets - The invention relates to a method for quality control of species used in analytical or diagnostic or therapeutic procedures. It comprises immobilization of a model of the malignancy to a solid support (10-22-2009
20100099202DETECTION OF VON-WILLEBRAND FACTOR (vWF) ACTIVITY - The present invention relates to a method for detecting von-Willebrand factor (vWF) activity comprising assaying a sample in the presence of a soluble form or portion of glycoprotein Ib(α) (GPIb(α)) and ristocetin, or a functionally equivalent substance. Additionally, the invention relates to the use of the aforementioned soluble form or portion of glycoprotein Ib(α), of ristocetin or a functional equivalent substance, of specifically reacting anti-GPIB(α) antibody(ies) and/or of specific binding partners, like specifically reacting anti-vWF antibody(ies) for carrying out the method of the invention. Furthermore, the present invention relates to kits for carrying out the method of the invention.04-22-2010
20110201132Optical Detection of Label-Free Biomolecular Interactions using Microreplicated Plastic - Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.08-18-2011
20090093069Topiramate Immunoassays - Diacetonefructose derivatives have substituents at the hydroxyl-position. Diacetonefructose derivatives may include immunogenic moieties to prepare anti-diacetonefructose derivative antibodies, or antigenic moieties for immunodiagnostic assays. Also, the diacetonefructose derivatives can include signal generating moieties for detecting the presence or amount of the diacetonefructose derivative in a sample. Additionally, the diacetonefructose derivatives can be used in immunodiagnostic assays to compete with topiramate for binding with anti-diacetonefructose derivative antibodies. Also, methods, compositions and kits are disclosed directed at diacetonefructose derivatives, immunogens, signal generating moieties and immunoassays for topiramate.04-09-2009
20110171754ANALYSIS SYSTEM - An analysis system comprises a sampling cartridge (07-14-2011
20090104716TARGET SUBSTANCE DETECTING ELEMENT, TARGET SUBSTANCE DETECTING APPARATUS, AND TARGET SUBSTANCE DETECTING METHOD - The present invention enables to classify and measure two or more kinds of target substances which have the same recognition site recognized by a specific capturing body. Specifically, by using a target substance detecting element for detecting two or more targets, whose kinds are different mutually, at the same time, the target substance detecting element, characterized by having a base material, two or more kinds of metal structures provided on a surface of the base material, and a target capturing body provided on each surface of the two or more kinds of metal structures, and in that the number of kinds of the metal structures are equal to or more than the number of kinds of the target substances, concentrations of the two or more kinds of target substances can be calculated by integrating and analyzing detection signals detected from the target substance detecting element.04-23-2009
20090104715Method for mixing two or more types of liquids in porous carrier - It is an object of the present invention to provide a method for simply mixing two or more types of liquids in a porous carrier. The present invention provides a method for mixing two or more types of liquids in a porous carrier, which comprises: laminating a first porous carrier consisting of an upstream portion and a down stream portion that are integrated with each other on a second porous carrier consisting of an upstream portion and a downstream portion that are integrated with each other, such that the upstream portion of the first porous carrier and the upstream portion of the second porous carrier form a bifurcated portion; adding a first liquid to the upstream portion of the first porous carrier; adding a second liquid to the upstream portion of the second porous carrier; and giving external force in a vertical direction towards the first and second porous carriers at a position wherein the first porous carrier is laminated on the second porous carrier on the side downstream of the bifurcation point of the bifurcated portion, so as to mix the first liquid with the second liquid in the first and second porous carriers.04-23-2009
20090291508NANOPARTICLES IN DIAGNOSTIC TESTS - The present invention includes methods and devices that detect target molecules in a biological sample. The sample analysis device of the present invention includes nanoparticles. In one embodiment, the nanoparticles are directly immobilized on the surface of the sample analysis device. In another embodiment, the nanoparticles are indirectly immobilized on the surface of the sample analysis device by incorporating them in appropriate media and immobilizing the nanoparticles within a matrix.11-26-2009
20090042317SUBSTANCE IMMOBILIZING APPARATUS, SUBSTANCE DETECTING APPARATUS AND SUBSTANCE IMMOBILIZING METHOD - The efficiency of the specific binding of a target substance to an immobilization region is increased. As a first step, target substance 02-12-2009
20080280378Grading of Immune Responses - The invention is a method for grading a polyclonal antibody response with respect to a desired 5 binding specificity. The response is provoked by an antigenic agent in a host, preferably a vertebrate host. The characteristic feature of the method comprises the steps of: (i) providing a reaction microcavity having an inlet end and an outlet end and containing a solid phase that exposes an immobilized binding structure (BS) that are capable of affinity binding antibodies (Ab) of the response, (ii) flowing a liquid sample containing antibodies of the response in the direction from the inlet end to the outlet end through the solid phase, (iii) determining the distribution of antibodies, which are captured during step (ii), along the flow direction of the solid phase, and (iv) grading the response based on the distribution determined in step (iii).11-13-2008
20080286882Method and Apparatus for Measuring the Concentration of a Ligand Contained in a Sample That Is To Be Tested - In a method for measuring the concentration of a ligand contained in a sample that is to be tested, at a plurality of test sites that are located on the surface of at least one substrate, in each case at least one receptor that is suitable upon contact with the ligand for specifically binding to said ligand, is immobilized. The sample is brought into contact for a predetermined time with the test sites in such a way that in the sample differently sized diffusion volumes are adjacent to the receptors from which at least one ligand can diffuse to the respective receptor during the predetermined time. After the predetermined time has passed, for each test site a measured signal for the number or density of the binding events at the test site is acquired.11-20-2008
20080293163Methods to identify compounds that modulate rage - Provided are methods to detect of modulators of the Receptor for Advanced Glycated Endproducts (RAGE). The invention comprises a method for detection of RAGE modulators comprising: adsorbing a RAGE ligand onto a solid surface; adding a compound of interest and a protein comprising RAGE or fragment thereof, to the preadsorbed ligand; adding an antibody which binds to RAGE or fragment thereof and a secondary antibody which binds to the anti-RAGE antibody; measuring the secondary antibody bound to the anti-RAGE antibody; and comparing the amount of RAGE bound to the ligand in the presence of varying amounts of the compound of interest. In an embodiment, the fragment of RAGE is sRAGE. In one aspect, the invention use of compounds detected by the method for treatment of AGE-related syndromes including complications associated with diabetes, kidney failure, lupus nephritis or inflammatory lupus nephritis, amyloidoses, Alzheimer's disease, cancer, inflammation, and erectile dysfunction.11-27-2008
20080248591Gas bubble biosensor - The present invention describes a biosening device and method. Specifically, binding of target analyte perturbs the surface of a sensor strip so that gas bubbles are generated in solution. Said gas bubbles may be detected for determination of analyte presence in sample.10-09-2008
20100304501BIO LAB-ON-A-CHIP AND METHOD OF FABRICATING AND OPERATING THE SAME - Disclosed is a bio lab-on-a-chip. The bio lab-on-a-chip is provided on a piezoelectric thin film on a substrate, and includes a sensing unit to sense a bio signal and a fluidic control unit which controls a transfer of a microfluid adjacent to the sensing unit. Provided is also a method of fabricating the bio lab-on-a-chip. The method includes the steps of forming a piezoelectric thin film, forming a sensing unit to sense a bio signal of a microfluid on the piezoelectric thin film, and forming a fluidic control unit located adjacent to the sensing unit.12-02-2010
20080206889Comparative multiple analyte assay - Methods for measuring the relative amount of two or more analytes of interest in a fluid sample, as well as kits useful in the methods, are disclosed. The methods involve assays that utilize a solid phase apparatus with a membrane having an application point and at least two sample capture zones having sample capture reagents; analyte binding particles or analyte coated particles; and assessment of a ratio of such particles arrested in capture zones, wherein the ratio is equal to, or inversely equal to, the relative amounts of the analytes of interest in the fluid sample.08-28-2008
20100144060Protein detecting device - A protein detecting device, which comprises: (1) a detecting unit having a bonding section, which has properties for specifically bonding to a protein to be detected, a detecting section for detecting the bonding of the protein to be detected to the bonding section, the detecting section being made up of a polynucleotide double strand and a charge separating group, and an electrode section detecting the change in electrical conductivity of, or amount of transferred charge in, the polynucleotide double strand modified by the bond of the protein, (2) a standard electrode, (3) a reference electrode, (4) a container for housing the detecting unit, the standard electrode and the reference electrode, and containing a sample solutions comprising the protein to be detected, and (5) a measuring unit for measuring the protein based on a signal detected in the detecting unit.06-10-2010
20100144061SEQUENTIAL SOLID PHASE IMMUNOASSAY AND SYSTEM THEREFOR - A sequential solid phase immunoassay and system is disclosed. The immunoassay utilizes the secondary antibody method for the detection of antibodies in a membrane-based test. The system comprises a test strip including a nitrocellulose membrane having an immobilized antigen in a capture zone on the membrane and a stabilized liquid secondary antibody conjugate. The sequential solid phase immunoassay is performed in a sequential manner with the addition of a fluid specimen being followed by the addition of the stabilized liquid secondary antibody conjugate. The sequential procedure using the system includes allowing the fluid specimen containing antibodies specific to the antigen to pass laterally from the test strip first end through the capture zone. The immobilized antigens in the capture zone capture antibodies specific to the antigen. The stabilized liquid secondary antibody conjugate then binds to the captured antibodies and can be detected visually or by a machine or reader.06-10-2010
20090061535Method of adjusting the working range of multi-analyte assay - The invention features a method of adjusting the concentration of at least one but not all of a plurality of analytes in a fluid sample to match a known working range of detection of an analyte assay system, where each of the plurality of analytes may or may not be present within an expected initial concentration range having a high end and a low end, and at least one analyte has a high end expected concentration range that exceeds the high end of the working range of the assay system. The expected concentration of the high concentration analyte is adjusted by a proportional scaling constant, α, so that the high end of the adjusted expected concentration range is less than or equal to the high end of the working range, without adjusting the expected concentration range of at least one other of the plurality of analytes. Adjustment is preferably accomplished by adding to the solution phase of the assay one or more scaling agents, each scaling agent binding with specificity to an analyte and thereby preventing it from being detected by the assay system, e.g., by competing with binding to immobilized capture agent. This scaling method contrasts with prior methods, in which a concentration of available analyte is offset by a fixed amount to adjust the detectable threshold of the assay. Here, the amount of scaling agent is proportional to a scaling coefficient, and the scaling agent is present in the solution phase of the assay at high concentrations relative to analyte. Due to the equilibrium conditions established by the laws of mass transfer, the amount of free analyte remaining in solution in the presence of scaling agent is predictable and finite, and can be measured as a quantitative indicator of the initial concentration of the analyte in the sample.03-05-2009
20110207237OPTICAL FIBER PROBE - A biosensor having an optical fiber having at least one curved portion configured to enhance penetration of evanescent waves; and one or more nanoparticles associated with the optical fiber, and configured to enhance localized surface plasmon resonance.08-25-2011
20100151593PREGNANCY AND SEX IDENTIFICATION TEST BASED ON SALIVA OR OTHER BODILY FLUIDS - A method of testing an animal for pregnancy or identifying the sex of the animal comprising the steps of first, providing a first vessel containing a liquid and having a removable surface wherein said removable surface is at least partially coated with an antibody and then introducing a bodily fluid from the female animal into said first vessel so that said bodily fluid contacts the liquid and then manipulating the first vessel so that the liquid contacts the antibody. Then, a second vessel containing a reporter hormone solution is provided and the removable surface from the first vessel is displaced to the second vessel and manipulating the second vessel so that the reporter hormone solution contacts the removable surface. Then, a third vessel containing an indicating solution which has an appearance which is related to the amount of the reporter hormone contacted is provided, and the removable surface is displaced from the second vessel to the third vessel. The third vessel is manipulated so that the indicating solution contacts the removable surface. Then, a determination is made regarding the pregnancy or sex based on the appearance of the indicating solution.06-17-2010
20080318341Dual Path Immunoassay Device - The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test site with immobilized antigens or antibodies or other ligand binding molecules such as aptamers, nucleic acids, etc. located at the junction of the first and second sorbent materials for identifying one or more ligands. The first and second sorbent strips touch each other at the test site location. The test cell may be used to test for pregnancy, HIV (including different HIV antigens or peptides), tuberculosis, prion, urin-analysis/drug, cardiac markers, cancer markers, Chagas, Chlamydia, dental bacteria (SM/LC), influenza A, influenza B, adenovirus, rotavirus, strep A, other bacteria or viruses, etc., and veterinary applications such as CPV, FIV, FeLV, heartworm, etc., although it is not limited to those applications.12-25-2008
20110223690ASSAY - The present invention provides an assay kit for detecting an analyte of interest in a sample. The kit comprises a) a reporter species; b) a labelled species having first and second binding regions, wherein the first binding region is capable of binding to the analyte of interest and the second binding region is capable of binding to the reporter species; c) an immobilised species capable of binding to the first binding region of the labelled species; and d) immobilised capture reagent capable of binding to the reporter species. The arrangement is such that the sample is contacted with the labelled species, is then contacted with the immobilised species and is subsequently contacted with the immobilised capture reagent, the reporter species being added prior to exposure of the sample to the immobilised capture reagent. If no analyte is present in the sample, the labelled species becomes bound to the immobilised species and is therefore unable to bind to the immobilised capture reagent. If analyte is present in the sample, the analyte binds to the labelled species such that the labelled species is unable to bind to the immobilised species but can bind to the immobilised capture reagent via the reporter species, the presence of the analyte thus being determined by the presence of labelled species bound to the immobilised capture reagent via the reporter species.09-15-2011
20100248394CONTROLLED FLOW ASSAY DEVICE AND METHOD - A device for handling liquid samples, comprising a flow path with at least one zone for receiving the sample, and a transport or incubation zone, said zones connected by or comprising an area having projections substantially vertical to its surface, said device provided with a sink with a capacity of receiving said liquid sample, said sink comprising an area having projections substantially vertical to its surface, and said sink being adapted to respond to an external influence regulating its capacity to receive said liquid sample.09-30-2010
20110143458TEST DEVICE FOR MEMBRANE ASSAY COMPRISING REFERENCE DISPLAY SECTION - A test device provided with a reference display section that rapidly and clearly indicates proper test completion with improved accuracy and stability is provided. Such test device is a test device for membrane assay using a specific binding reaction of a substance to be detected with a capture reagent immobilized on a membrane carrier and a reagent labeled with a labeling substance, which comprises a reference display section for indicating proper test completion on which a cationic polymer for capturing a labeled reagent has been immobilized.06-16-2011
20090191648Method and device to detect the presence of analytes in a sample - Disclosed are methods and apparatus useful for determining the presence or absence of one or more analytes in a liquid sample, such as a biological or environmental sample. In some embodiments, the method can use an indirect competitive immunochromatographic test strip.07-30-2009
20090221101Method for Adding an Apparent Non-Signal Line to a Rapid Diagnostic Assay - A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (−) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (−) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.09-03-2009
20090258441METHOD OF PROVIDING PARTICLES HAVING BIOLOGICAL-BINDING AREAS FOR BIOLOGICAL APPLICATIONS - The present invention includes micro-sphere composition, methods of making binding assays. The present invention also includes a micro-sphere for binding biological molecules without pretreatment. The micro-sphere includes a spherical glass substrate having one or more metal nanoparticle regions that are exposed from within the glass, wherein the micro-sphere is capable of binding biological molecules without pretreatment.10-15-2009
20090246888NANOASSAYS - The present invention provides assays of nanometer-level dimension.10-01-2009
20090258440SURFACE FOR LABEL INDEPENDENT DETECTION AND METHOD THEREOF - A functional group and spacer group modified polymer composition, articles incorporating the composition, and methods for label-independent-detection using the articles, as defined herein.10-15-2009
20100015727METHOD FOR DETERMINING TRANSPORT ACTIVITY OF A TRANSPORT PROTEIN - The present invention relates to a method for determining transport activity of a transport protein and its use for the identification of compounds which can modulate said transport activity.01-21-2010
20100184237Chemical reaction device, chemical reaction system, and chemical reaction method - A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.07-22-2010
20100261292Methods for Conducting Assays - The invention relates to methods for conducting solid-phase binding assays. One example is an assay method having improved analyte specificity where specificity is limited by the presence of non-specific binding interactions.10-14-2010
20100261293DIAGNOSTIC DETECTION DEVICE - The invention comprises a device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion. In specific embodiments, the device comprises a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising a gold colloid, preferably having a mean particle size of 50 nm to 100 nm. In further embodiments, the device comprises a capture component comprising polymerized streptavidin. The diagnostic device is particularly useful in the preparation of pregnancy test kits.10-14-2010
20100227419LAB-ON-A-CHIP AND METHOD OF DRIVING THE SAME - Provided is a lab-on-a-chip. The lab-on-a-chip includes a first region where a lower substrate and an upper substrate are bonded to each other, a second region where the lower and upper substrates are not bonded, and a gap adjusting member disposed at an end of the second region that is opposite to a boundary between the first and second regions, the gap adjusting member being configured to adjust a gap between the first and second substrates to control a capillary force.09-09-2010
20110059556Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices - The compositions and methods described herein are designed to introduce functionalized microparticles into droplets that can be manipulated in microfluidic devices by fields, including electric (dielectrophoretic) or magnetic fields, and extracted by splitting a droplet to separate the portion of the droplet that contains the majority of the microparticles from the part that is largely devoid of the microparticles. Within the device, channels are variously configured at Y- or T junctions that facilitate continuous, serial isolation and dilution of analytes in solution. The devices can be limited in the sense that they can be designed to output purified analytes that are then further analyzed in separate machines or they can include additional channels through which purified analytes can be further processed and analyzed.03-10-2011
20100120173IMMUNOCHROMATOGRAPHIC STRIP DISC FOR MULTIPLEXED DETECTION AND DETECTION METHOD USING THE SAME - Provided is a strip-assembled immunochromatographic disc, containing: a base, a lid engaged with the base and a draining piece disposed between the strips on the base and the lid, wherein a sampling opening is disposed on the lid directly facing to the draining piece, and the said sampling opening intercommunicates to a draining groove provided on the inner side of the lid which is formed by a plurality of draining channels; several strip stages are provided on the base with their location and number corresponding to those of the draining channels provided on the lid, and the edge of the draining piece laps to the sample pads of the strips carried on the stage adjacent to one end of the sampling opening. Also provided is a method of performing multiplexed immnochromatographic detection using the strip disc to accomplish the detection of multiple target analytes in one sample in an assay.05-13-2010
20100210039SANDWICH IMMUNOASSAY AND MONOCLONAL ANTIBODIES FOR COMP, CARTILLAGE OLIGOMERIC MATRIX PROTEIN - The present invention provides a new sensitive direct sandwich assay for determining the presence of COMP in a clinical sample. Two monoclonal antibodies directed against separate antigenic determinants of the COMP molecules are used in the assay. The invention also relates to three particularly advantageous monoclonal antibodies per se that are directed against human COMP. Cell cultures manufacturing these antibodies have been deposited according to the Budapest Treaty at Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, and have been assigned accesion numbers DSM ACC2406, DSM ACC2408 and DSM ACC2418, respectively. A diagnostic kit comprising at least two of these monoclonal antibodies also constitute a part of the present invention.08-19-2010
20090209049USE OF METAL COMPLEXES - A method of immobilising a target molecule on a substrate, which comprises exposing the target molecule to the substrate in the presence of a metal complex, wherein the target molecule is an unmodified target molecule, and wherein the metal complex is selected to provide a stable binding interaction between the target molecule and the substrate.08-20-2009
20080305558Cancer Screening Test - The invention provides a cancer screening test, to identify patients having an increased likelihood of cancer, comprising the step of determining the presence or absence of members of a test group of tumour associated antigens in the blood of a patient. The test group comprises a plurality of tumour-associated antigens. Antigens of particular interest include Vascular Endothelial Growth Factor-A (VEGF A); CEA 125 (Carcinoembryonic antigen 125); Prostate Specific Antigen (PSA); CA15-3 (Cancer antigen 15-3); CA125 (Cancer Antigen 125); CYFRA21-1 (Cytokeratin-19 fragments); Soluble ectodomain of c-erbB2; CA27.29 (Cancer Antigen 27.29); IGF-I (Insulin-like growth factor-1); IGF-2 (Insulin-like growth factor-2); and IGFBP-3 (Insulin-like growth factor binding protein 3). The invention also provides an antibody-based test kit to implement the screening method.12-11-2008
20080274566Method and assay for detection of residues - Embodiments described herein include methods and assays for detecting an analyte in a sample using a plurality of control zone capture agents. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.11-06-2008
20110117676MAGNETIC SENSOR, PRODUCTION METHOD OF THE SAME, AND TARGET SUBSTANCE DETECTING APPARATUS AND BIOSENSOR KIT USING THE SAME - The present invention provides a magnetic sensor which detects a target substance indirectly by making a labeling substance larger than the target substance bond with the target substance contained in a sample in a detection area, and detecting the labeling substance, comprising a capture area which is relatively easy to capture the target substance, and a non-capture area which is relatively hard to capture the target substance, on a surface of a member which is comprised in a detection area, wherein the capture area is surrounded by the non-capture area. Thereby, the sensor enables to detect comparatively accurately the number and concentration of substances which cannot be directly detected, and enables to be used for detection of various target substances.05-19-2011
20110117675SUBSTANCE IMMOBILIZING APPARATUS, SUBSTANCE DETECTING APPARATUS AND SUBSTANCE IMMOBILIZING METHOD - The efficiency of the specific binding of a target substance to an immobilization region is increased. As a first step, target substance 05-19-2011
20110117673METHODS AND SYSTEMS TO COLLECT AND PREPARE SAMPLES, TO IMPLEMENT, INITIATE AND PERFORM ASSAYS, AND TO CONTROL AND MANAGE FLUID FLOW - Methods and systems to related to sample collection, assays, and fluid control and management. Methods and systems disclosed herein, and portions thereof, may be implemented alone and/or in various combinations with one another.05-19-2011
20110151585DUAL INLET MICROCHANNEL DEVICE AND METHOD FOR USING SAME - A dual inlet microchannel device and a method for using the device to perform a flow-through kinetic assay are described. A microplate having an array of the dual inlet microchannel devices and in particular their specially configured flow chambers is also described. Several embodiments of the dual inlet microchannel devices and specially configured flow chambers are also described.06-23-2011
20110008914Biomarkers for the Diagnosis and Treatment of Pancreatic Cancer - Compositions and methods which indicate an increased risk for pancreatic carcinoma are disclosed.01-13-2011
20110244598Test Element Having Combined Control and Calibration Zone - A test element, for example in the form of an immunological test strip functioning according to the sandwich principle, for the fluorophoric detection of one or more analytes in a sample comprising an analyte detection zone and a combined control and calibration zone. The combined control and calibration zone include a fluorophore and binding partners for the specific binding of reagents labelled with a fluorophore. Furthermore, the invention concerns a method for calibrating an analyte-specific measurement signal, a method for determining the concentration of an analyte in a sample and the use of the test element for calibrating a signal generated in the analyte detection zone of a test element.10-06-2011
20110033953CHARACTERIZATION OF REACTION VARIABLES - A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).02-10-2011
20110117672MAGNETIC IMMUNOCHROMATOGRAPHIC TEST METHOD AND DEVICE - Method for detecting and quantifying an analyte in a liquid sample, using a test strip and magnetic particles as a detectable label and based on the detection of an amount of magnetic particles which become bound to a reading zone of a test strip as a result of performing the method, said amount being linked through a function to the analyte content of the sample, wherein the magnetic particles exhibit a nonlinear magnetization characteristic, which test strip is made of porous material and is arranged inside a case, cartridge or the like to form an assay device, wherein in its part surrounding the reading zone, the case is made of a material that is permeable to magnetic field, wherein for the reading of the reaction, the assay device is positioned in a measure cell of a magnetic reading device which detects the amount of magnetic particles in the reading zone by submitting it to at least one excitation magnetic field having about 90% of its power within one frequency band or a plurality of frequency bands, and the reading device measures the induced magnetic response field outside said frequency band or said plurality of frequency bands.05-19-2011
20110244597IMMUNOCHROMATOGRAPHIC MEDIUM AND IMMUNOCHROMATOGRAPHIC METHOD - Disclosed are an immunochromatographic medium and an immunochromatographic method in which, even when plural items exceeding four items are detected which are the number of colors that can be detected by colored latex particles, the plural items can be detected and quantified simultaneously. The immunochromatographic medium comprises a support, a label-attached reagent immobilized on a labeling agent, and detection portions on which a material capable of combining with a substance to be detected in a sample is immobilized, the label-attached reagent and the detection portions being provided on the support, featured in that the labeling agent is a phosphor nanoparticle and the detection portions are shaped in the form of dots.10-06-2011
20110244596PULSED MAGNETIC ACTUATION FOR SENSITIVE ASSAYS - A method for controlling the movement of magnetic or magnetizable objects (10-06-2011
20110086438BIOSENSOR UTILIZING A RESONATOR HAVING A FUNCTIONALIZED SURFACE - Systems and methods for detecting the presence of biomolecules in a sample using biosensors that incorporate resonators which have functionalized surfaces for reacting with target biomolecules. In one embodiment, a device includes a piezoelectric resonator having a functionalized surface configured to react with target molecules, thereby changing the mass and/or charge of the resonator which consequently changes the frequency response of the resonator. The resonator's frequency response after exposure to a sample is compared to a reference, such as the frequency response before exposure to the sample, a stored baseline frequency response or a control resonator's frequency response.04-14-2011
20090311805ASSAY DEVICE AND METHOD - There is disclosed an analysis device for the analysis of a liquid sample, said device comprising a substrate, said substrate at least partly having projections substantially vertical to the surface of said substrate, and having a height (H12-17-2009
20110177620BIOANALYTICAL ASSAY - A nanoparticle having a detectible feature and whose diameter is less than 200 nm, and which is coated with multiple specific binding reactants such that the affinity constant of the nanoparticle towards an analyte exceeds that of free binding reactant towards the analyte and/or the association rate constant between the nanoparticle and the analyte exceeds the association rate constant between the free binding reactant and the analyte. Also disclosed is a homogenous assay based on a first group labeled with a luminescent energy donor nanoparticle and a second group labeled with an energy acceptor compound, where the donor has a long excited state lifetime, and the increase or decrease, respectively, in the energy transfer from the donor to the acceptor resulting from shortening or lengthening, respectively, of the distance between these groups, is measured.07-21-2011
20100068825Method and Device for Detecting at Least One Property of at Least One Object with a Microchip - The present invention relates to a method and a device for the detection of at least one property of at least one object. The detection is effected by means of a microchip. The microchip has at least one readable detection pixel. In order to reduce the technical equipment outlay during object detection, the method according to the invention is characterized by the fact that the at least one object is arranged at the microchip in a spatially predetermineable position. The at least one object is exposed to illumination light in order to detect the illumination light that interacts with the at least one object or the light that is induced by the illumination light and emerges from the at least one object by means of the at least one readable detection pixel of the microchip.03-18-2010
20110151584Dual Path Immunoassay Device - The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test site with immobilized antigens or antibodies or other ligand binding molecules such as aptamers, nucleic acids, etc. located at the junction of the first and second sorbent materials for identifying one or more ligands. The first and second sorbent strips touch each other at the test site location. The test cell may be used to test for pregnancy, HIV (including different HIV antigens or peptides), tuberculosis, prion, urin-analysis/drug, cardiac markers, cancer markers, Chagas, Chlamydia, dental bacteria (SM/LC), influenza A, influenza B, adenovirus, rotavirus, strep A, other bacteria or viruses, etc., and veterinary applications such as CPV, FIV, FeLV, heartworm, etc., although it is not limited to those applications.06-23-2011
20110250705NANOPORE CAPTURE SYSTEM - A nanopore capture system may include a material configured to pass through a nanopore device in a controlled manner based upon its interaction with the nanopore device. The system may also include a capture mechanism connected to one end of the material. The capture mechanism may be configured to catch a particular type of molecule while ignoring other types of molecules. The system may also include a controller to manipulate and/or detect the particular type of molecule.10-13-2011
20080248590Device For Carrying Out A Biological Assay - An integrated lab-on-a-chip device for carrying out an assay to detect the presence of a biological molecule in a fluid sample, the device comprising: (a) an inlet for a fluid sample; (b) one or more reaction sites each in fluid communication with the inlet; (c) one or more reagent reservoir systems each containing reagents required for an assay to detect a biological molecule, the reagents being arranged sequentially in each reservoir system in the order in which they are required for the assay and separated from one another by a fluid.10-09-2008
20110177619IN VITRO DIAGNOSTIC MARKERS COMPRISING CARBON NANOPARTICLES AND KITS - This invention relates to luminescent markers for in vitro diagnostic applications, and kits using those markers. In some embodiments, those markers comprise luminescent carbon nanoparticles. Some embodiments provide a method for investigating an analyte comprising correlating a marker to the analyte and observing the luminescence from the marker, wherein the marker comprises a nanoparticle having a carbon core. In vitro kits, including those employing a marker comprising a nanoparticle having a carbon core, are also provided.07-21-2011
20090111196Immunochromatography method - A object of the present invention is to provide an immunochromatography method that makes it possible to rapidly detect an ultratrace amount of an analyte that has been impossible to analyze by conventional immunochromatography methods. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance which is modified with a first binding substance against the analyte in a mixed state on a porous carrier and capturing the analyte and the label at a reaction site on the porous carrier having a second binding substance against the analyte or a substance capable of binding to the first binding substance against the analyte, so as to detect the analyte, wherein the labeling substance having an average particle size of 1 μm or more and 20 μm or less is detected.04-30-2009
20080268550MOLECULARLY-IMPRINTED CHEMICAL DETECTION DEVICE AND METHOD - A novel method of molecular imprinting is described. Using a modified soft lithography technique, a molecularly-imprinted chemical detection device comprising at least one molecularly-imprinted polymer capable of detecting at least one chemical target is produced. The device can be used in the field for in situ detection and quantification of chemical targets using standard surface analytical techniques.10-30-2008
20080254552Methods and devices for analyte detection - Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.10-16-2008
20110053292 SIGNAL AMPLIFICATION TECHNIQUE FOR MASS ANALYSIS - There is provided a novel method for amplifying mass spectrometric signals. More particularly, a novel method for detecting signals of a target molecule includes: i) allowing a test sample, in which it is required to determine whether or not a target molecule is present, to be contact with a gold particle whose surface is modified to selectively bind to the target molecule, ii) allowing a low molecular molecule engrafted to the gold particle to generate mass spectrometric signals after the interaction, such as binding, between the gold particle and the target molecule, and iii) amplifying the mass spectrometric signals by generating a great deal of mass spectrometric signals of the low molecular molecule even in the presence of a trace of the target molecule. Also, the assay system using the method and the gold particle prepared in the method are provided. The method may be useful to specifically amplify signals of the target molecule without any pretreatment of a test sample, which makes it possible to measure the target molecule simply and precisely.03-03-2011
20110027914SAMPLE PLATE SYSTEMS AND METHODS - A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. Also provided herein is a method of using the sample plate and kits comprising the sample plate.02-03-2011
20110027913MULTIPLEXED NANOSCALE ELECTROCHEMICAL SENSORS FOR MULTI-ANALYTE DETECTION - Provided are nanoscale devices suitable for multiplexed, parallel detection of multiple analytes and methods for fabricating such devices.02-03-2011
20110117674ASSAY METHOD AND DEVICE - A method for the analysis of at least two analytes in a liquid sample, in which a substrate is provided wherein at least two different types of capturing molecules are immobilized on the substrate and wherein each type of capturing molecule has specific affinity for an analyte. The sample is contacted with capturing molecules, wherein for at least one analyte to be analyzed contact is induced between the capturing molecules and a labelled detection molecule with specific affinity for the analyte, and for at least one another analyte to be contact is induced between the capturing molecules and a labelled version of the analyte. A detectable signal is measured from the labelled detection molecule and the labelled analyte on the substrate, wherein the concentration of the labelled analyte is adapted to the concentration of the analyte in the sample.05-19-2011
20110136264METHOD FOR DETECTING TARGET SUBSTANCE AND TARGET-SUBSTANCE DETECTION KIT - The present invention provides a method for detecting a target substance by detecting the presence or concentration of a target substance in a sample solution by bringing the sample solution into contact with a detecting element including a detecting part and a non-detecting part and detecting the presence or number of a magnetic label (magnetic marker) present in the vicinity of the surface of the detecting part and provides a target-substance detection kit. The surface potential ψ06-09-2011
20110136263MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER - Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.06-09-2011
20100330702ULTRASENSITIVE DETECTION OF BIOMOLECULES USING IMMUNOSEPARATION AND DIFFRACTOMETRY - Systems and methods for rapid and ultrasensitive detection of target hiomolecules in a sample are presented. The detection of biomolecules is achieved through a synergistic use of immunoseparation and diffractomctry, and the formation of antibody-biomolecule-ligand sandwich complexes that form diffraction gratings. Characteristic diffraction patterns are then produced upon illumination of the diffraction gratings with light. The diffraction patterns can he used to detect very low amounts of biomolecules present in the sample.12-30-2010
20100330705Immunological Test Element with Improved Control Zone - The invention concerns a test element for carrying out an immunological sandwich test for determining an analyte from a liquid sample containing a reagent zone or conjugate zone which contains a conjugate of an analyte binding partner and a label which can be detected directly or indirectly by visual, optical or electrochemical means (e.g., an enzyme, fluorescent or direct label, etc.) wherein the conjugate can be dissolved by the liquid sample, a detection zone which contains a permanently immobilized (i.e., which cannot be detached by the liquid sample) binding partner for the analyte or for complexes containing the analyte; and a control zone which contains a permanently immobilized binding partner for the conjugate of analyte binding partner and label characterized in that the control zone additionally contains one or more permanently immobilized binding partner(s) for the analyte or for complexes containing the analyte.12-30-2010
20100330704COMPOSITE PARTICLE, METHOD FOR PRODUCING THE SAME, DISPERSION SOLUTION, MAGNETIC BIOSENSING APPARATUS AND MAGNETIC BIOSENSING METHOD - To provide a method for producing composite particles small in particle size, excellent in mono-dispersibility, high in magnetic-substance content per particle, large in saturation magnetization, excellent in dispersion stability and having non-specific adsorption suppressibility.12-30-2010
20100330703ASSAYS INVOLVING COLORIMETRIC AND OTHER SIGNALING - The present invention generally relates to particles and, in particular, to methods of determining binding involving particles, e.g., using colorimetric and other signaling techniques. In one aspect, a mixture of particles of different colors (e.g., at least a first color and a second color) is provided that exhibits a first collective color, e.g., due to the presence of the different colors of particles within the mixture. The mixture can then be exposed to a medium containing a binding partner able to preferentially bind to some of the particles, e.g., particles of a first color relative to particles of a second color. The bound particles can be separated in some fashion (e.g., filtration, gravity, magnetism, centrifugal separation, etc.), such that the mixture exhibits a second collective color, e.g., due to the presence of a greater number of particles of the second color relative to the number of particles of the first color. Accordingly, by visualizing or otherwise determining a color change, a binding event may be determined. Other aspects of the invention relate to kits involving such particles, methods of promoting the making or use of such particles, or the like.12-30-2010
20110033952Sensor for Biomolecules - A sensor for biomolecules includes a silicon fin comprising undoped silicon; a source region adjacent to the silicon fin, the source region comprising heavily doped silicon; a drain region adjacent to the silicon fin, the drain region comprising heavily doped silicon of a doping type that is the same doping type as that of the source region; and a layer of a gate dielectric covering an exterior portion of the silicon fin between the source region and the drain region, the gate dielectric comprising a plurality of antibodies, the plurality of antibodies configured to bind with the biomolecules, such that a drain current flowing between the source region and the drain region varies when the biomolecules bind with the antibodies.02-10-2011
20100144059ANALYSIS DEVICE - There is provided an analysis device comprising a gas phase and a liquid phase and at least one sensor, said sensor having at least one point where an analyte is detected, said at least point being in contact with the liquid phase, characterised in that the device comprises a membrane with a first and a second side, which membrane is in contact with the gas phase on at least a part of one side of the membrane and which membrane is in contact with the liquid phase on at least a part of the other side of the membrane, wherein the membrane comprises openings, and wherein the largest possible distance between any two openings in the membrane is larger than the distance between the membrane and the point where an analyte is detected, moreover there is provided a method for analysing an analyte in a gas phase.06-10-2010
20090203152Method for Analyzing an Analyte Qualitatively and Quantitatively - A specific binding analysis method capable of controlling the amount of specific binding to freely setting the sensitivity, concentration range and the like in an analysis, and a device used therefore are provided. In order to optimize the amount of specific binding, the specific binding analysis method and the device used therefor restrain the amount and velocity of a sample passing, by capillarity, through a detection zone, by controlling the dimensions, ventilation resistance, hydrophilicity and the like in the portion of the device where the sample passes through.08-13-2009
20120309111BIOSAMPLE PLATE WITH DATA STORAGE AND WIRELESS COMMUNICATION MEANS - Embodiments of the disclosure relate to a biosample plate that includes a memory component for storing information related to the biosample, biosample plate and biosample analysis data, and a wireless communication interface for transferring information to and from the biosample plate. The biosample plate may be used with an analyzing and data recording system such as an electromagnetic tape drive. The disclosed biosample plate facilitates the correlation between a large number of biosample plates and data as data remains with the corresponding biosamples both when the biosample plates are in use and when they are in storage. The wireless communication interface may comprise an antenna disposed in a biosample plate for data transmission to and from the biosample plate by radio signals.12-06-2012
20100190269DEVICE, SYSTEM AND METHOD OF DETECTING TARGETS IN A FLUID SAMPLE - The present invention provides a biochemical detection system that comprises an exchangeable cartridge unit with light guiding tubes pre-coated with capture agent(s) and an optical detection unit. Upon flowing the liquid or gaseous sample containing the target(s) through the cartridge unit, the target(s) bind(s) to the capture agent(s) and is (are) detected by the amount of light or the variation of its properties while guided through the tubes. The optical detection unit is comprised of a light emitting element(s), a light connecting element(s) and a light detecting element(s) that delivers the amount of target(s) in the sample under investigation.07-29-2010
20100029015METHOD OF COUPLING BINDING AGENTS TO A SUBSTRATE SURFACE - The present invention relates to a method of coupling multiple binding agents to respective areas of a substrate surface by hydrodynamic addressing, using two laminar fluid flows that flow together in the same direction over the substrate surface with an interface to each other to successively couple the binding agents to the substrate areas, wherein each successive coupling of a binding agent to a surface area is followed or preceded by selective deactivation or activation of a selected surface area according to a defined protocol. The invention also relates to the use of such a binding agent-coupled substrate surface for analytical purposes.02-04-2010
20110070664Integrated Microfluidic Device for Serum Biomarker Quantitation using Either Standard Addition or a Calibration Curve - Apparatus and method for determining concentration of one or more target compounds in a sample solution containing one or more nontarget compounds that uses an affinity column to immobilize the target compounds. The target compounds are eluted and passed through a separation/detection system to determine quantitative measure of concentration for each of the target compound.03-24-2011
20110104824MULTI-CHANNEL SAW SENSOR CHIP - A sensor chip for specific analysis of analytes in a liquid includes a plurality of sensor elements based on the SAW principle and applied as layer structures onto a surface of a substrate. A surface of each sensor element is coated with a sensitive substrate or coating having receptors specifically binding one analyte. During operation, the surface of the sensor chip rests against and seals a half-open covering part, with the covering part and the sensor chip forming a flow cell to be rinsed by the liquid. Conductor structures contact the sensor elements from the flow cell. More than six sensor elements are present which can each be triggered and read out separately via control and measuring electronics. At least two and in particular all sensor elements, are each coated with a differently sensitive substrate and the sensitive substrate is applied to the surface before attachment of the cover part.05-05-2011
20100210037Microfluidic Device - A method of controlling the flow of an aqueous fluid in a microfluidic device, which makes use of a fluid gate comprising a UCST (upper critical solubility temperature) polymer and which allows fluid to flow by raising the temperature of the fluid gate above the USCT. Also provided is a device incorporating such a fluid gate and the use of such a device to detect an analyte in an aqueous fluid sample.08-19-2010
20100267168METHOD FOR END TITRE DETERMINATION AND THE EVALUATION THEREOF BY MEANS OF AN INDIRECT IMMUNOFLURESCENCE ASSAY - The invention relates to a method for end-titre determination in the determination of antibodies against nuclear and cytoplasmic antigens in human sera by means of an indirect immunofluorescence assay. The invention further relates to a kit for in vitro diagnosis for determining antibodies against nuclear and cytoplasmic antigens in human sera by means of an indirect immunofluorescence assay and a computer program for evaluation and for determination of the end titre within the framework of said method.10-21-2010
20100285611PHOTOBLEACHING RESISTANT PH SENSITIVE DYE NANOREACTORS WITH DUAL WAVELENGTH EMISSION - A pH sensitive nanoreactor can include an aqueous core within a liposome. The aqueous core can include a pH responsive dye dispersed or dissolved within the core. The liposome provides a nanoscale environment for the dye. Further, a nanoshell can be present which encapsulates the liposome. The nanoshell can be permeable to hydrogen ions while also protecting the dye from exposure to deleterious compounds and photobleaching.11-11-2010
20110111531METHOD AND UNIT FOR DETECTION OF INTERACTIONS OF BIOLOGICALLY RELEVANT MOLECULES - The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: 05-12-2011
20080220537Substrates and methods for selective immobilization of active molecules - Substrates and methods for providing increased selectivity in the immobilization of active molecules of interest in desired locations of substrates for use in analytical operations and particularly optical analytical operations.09-11-2008
20100129936IMMUNOASSAY EMPLOYING TWO-STEP INTERNAL CALIBRATION REACTION - Methods for quantitatively measuring the amount of an analyte of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods comprise sandwich assays, and utilize an internal calibration reaction that closely mimics the reaction of test particles by the use of a two-step reaction.05-27-2010
20090221102METHOD FOR ADDING AN APPARENT NON-SIGNAL LINE TO A LATERAL FLOW ASSAY - A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (−) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (−) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.09-03-2009
20090176319Calibrator For Immunoassays - The invention generally relates to the field of immunoassays. In particular, the invention relates to use of a calibrator material to calibrate immunoassays for autoantibodies.07-09-2009
20100081214CHROMATOGRAPHIC ASSAY SYSTEM - The present application discloses an analyte detection apparatus having at least one reservoir area and a wicking membrane, wherein a labeled specific binding partner is impregnated on the reservoir area; and a region on the wicking membrane where at least one chemical component is immobilized.04-01-2010
20080227219Electrochemiluminescent assay - Disclosed herein are compositions that may be used in an assay, such as an immunoassay, for detecting and/or quantifying at least one analyte of interest, such as an antigen. Also disclosed are control/calibrator compositions and methods for preparing control/calibrator compositions that may be used in assays, such as immunoassays, methods for detecting and/or quantifying an analyte with the compositions, and kits containing the compositions.09-18-2008
20120003756CHROMATOGRAPHIC ASSAY SYSTEM - The present application discloses an analyte detection apparatus having at least one reservoir area and a wicking membrane, wherein a labeled specific binding partner is impregnated on the reservoir area; and a region on the wicking membrane where at least one chemical component is immobilized.01-05-2012
20110097820Swab-Based Diagnostic Systems - A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.04-28-2011
20120208299Reduced Step Dual Path Immunoassay Device and Method - Test cells have a first sorbent strip with a sample receiving location and defining a first migration path, a distinct second sorbent strip which receives buffer solution and at least partially defines a second migration path distinct from and elongated relative to the first migration path, conjugate supported by the second strip, a test site located at a junction of the first and second strips and having an immobilized ligand-binding mechanism, and a divider which directs a first amount of the buffer to the first strip to move the sample to the test site and a second amount to the second strip to move the conjugate to the test site. The first and second migration paths have first and second lengths chosen so that ligand in the sample reaches the test site and binds to the immobilized ligand-binding mechanism prior to the conjugate reaching the test site.08-16-2012
20100184238REACTION APPARATUS AND PROCESS - A new reaction apparatus including a capillary having an inner surface to which a probe molecule that specifically binds to an analyte is immobilized, allowing a short throughput time for completing the binding reaction, and achieving a highly efficient reaction using a small amount of a sample and a process of the reaction are provided. The reaction apparatus includes a capillary having an inner surface to which a probe molecule that specifically binds to an analyte is immobilized; a columnar magnetic body that is disposed in a fluid containing the analyte in the state that the fluid is placed in the capillary; end-fixing means for fixing one end of the columnar magnetic body in the capillary by a DC magnetic field; and end-moving means for moving the other end of the columnar magnetic body by an AC magnetic field so as to transfer the fluid.07-22-2010
20100093108Lung cancer diagnotic assay - A diagnostic assay for determining presence of lung cancer in a patient depends, in part, on ascertaining the presence of an antibody associated with lung cancer using random polypeptides. The assay predicted lung cancer prior to evidence of radiographically detectable cancer tissue.04-15-2010
20090130776BINDING PROTEIN MOLECULE - A binding protein molecule, characterized in that it has a first domain having a binding site to an inhibitor of non-specific adsorption in which the domain comprises a part of the variable region of an antibody as the binding site and a second domain having a binding site to a target substance in which the domain comprises a part of the variable region of an antibody as the binding site, wherein the first and second domains are bound via a linker.05-21-2009
20110183439STRUCTURES INCORPORATING CONFORMATIONALLY FLEXIBLE CONJUGATED POLYMERS AND METHODS OF USE - Methods, compositions and articles of manufacture involving conformationally flexible conjugated polymers are provided. A structure is provided comprising the conformationally flexible conjugated polymer bound to or associated with at least one member of a binding pair comprising a sensor molecule and a target molecule or the complex they form. The conformationally flexible conjugated polymer comprises at least one angled linker having bonds to its two adjacent polymeric units which form an angle of less than about 155° with respect to one another. Methods of use of such structures and solutions comprising them are also provided.07-28-2011
20120129272LATERAL FLOW ASSAY SYSTEMS AND METHODS - In one aspect, a diagnostic test system includes a housing, a reader, and a data analyzer. The housing includes a port for receiving a test strip. The reader obtains separable light intensity measurements from localized regions of an area of the detection zone exposed for optical inspection, wherein each of the localized regions is characterized by at least one surface dimension smaller than the first dimension. The data analyzer identifies ones of the light intensity measurements obtained from the at least one test region and computes at least one parameter from the identified ones of the light intensity measurements. In another aspect, the reader obtains a respective set of light intensity measurements from each of multiple corresponding regions of the exposed surface area of the detection zone, and the data analyzer computes at least one parameter from at least one of the sets of light intensity measurements.05-24-2012
20100210038DRY REAGENT PARTICLE ASSAY AND DEVICE HAVING MULTIPLE TEST ZONES AND METHOD THEREFOR - The present invention relates to a dry reagent assay device having at least one test zone and at least one reference zone, which provides an internal mechanism for assuring correct and reliable assay procedures and reagent qualities. In one embodiment, the present invention relates to an assay device having, at least one test zone for detecting at least one analyte in a sample by reacting the sample with a labeled indicator reagent, and a reference zone for receiving, unreacted labeled indicator reagent.08-19-2010
20100173430CYSTEINE-TAGGED STAPHYLOCOCCAL PROTEIN G VARIANT - The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capability, compared with an untagged protein G variant.07-08-2010
20100047928TARGET SUBSTANCE DETECTION ELEMENT, TARGET SUBSTANCE DETECTION METHOD, AND METHOD FOR PRODUCING TARGET SUBSTANCE DETECTION ELEMENT - It is intended to provide a target substance detection element wherein a target substance capturing body for capturing target substances is immobilized with good orientation in a desired region on the surface of the target substance detection element, a method for producing the target substance detection element, and a detection method using the target substance detection element. The present invention provides a target substance detection element for detecting the presence or absence or concentration of a target substance in a sample, characterized in that: the target substance detection element includes at least a detection substrate including plural layers and a target substance capturing body immobilized on the surface of the detection substrate; the target substance capturing body has at least a first peptide region specifically recognizing a first layer of the plural layers constituting the detection substrate and binding to the first layer and a second peptide region specifically recognizing a second layer different from the first layer of the plural layers and binding to the second layer; and the first layer and the second layer are adjacent to each other.02-25-2010
20100047927KIT FOR MEASUREMENT OF TERMITE INSECTICIDE ACTIVE INGREDIENT BY IMMUNOASSAY METHOD - A kit and a method are provided for easily measuring the concentration of an active ingredient of a termite insecticide persisting in soil, particularly on site where a termite insecticide was actually applied. A kit of the present invention comprises 1) an extraction unit for extracting, with an solvent, a termite insecticide active ingredient from an object of measurement and 2) a reaction unit including a reaction container for encapsulating an identifying antigen, a fixing member for immobilizing an antibody against an active ingredient, and a sealing member capable of fitting to the reaction container. The kit optionally includes 3) a detection unit for visually or optically detecting a change depending on the concentrations of the active ingredient in the object of measurement, and 4) a dilution unit for diluting the sample solution to a certain ratio.02-25-2010
20090061534Assay Device with Shared Zones - Disclosed is an assay device for determining the presence and/or extent of one or more analytes in liquid sample containing a) first and second assays each comprising a flow-path having a detection zone for immobilising a labelled binding reagent, wherein detection of a labelled binding reagent at one or both detection zones is indicative of the presence and/or extent of one or more analytes; b) a shared reference zone; c) one or more light sources to illuminate the detection zones and the reference zone; d) one or more photodetectors to detect light from the detection zones and the reference zone, which photodetector/s generate a signal, the magnitude of which signal is related to the amount of light detected; and e) signal processing means for processing signals from the photodetector/s.03-05-2009
20090061533STRUCTURE, TARGET SUBSTANCE DETECTION ELEMENT AND TARGET SUBSTANCE DETECTION KIT - A target substance detection element that can effectively prevent the nonspecific adsorption of a target substance or impurities and detects the target substance with high sensitivity, a target substance detection kit, and a structure constituting the target substance detection element. The structure has a substrate, polymers present on the substrate surface, and first target substance capturing molecules bonded to the polymers. The polymer is composed of a polymer of a carboxybetaine monomer represented by General Formula (1) below. The first target substance capturing molecules are bonded to some of the carboxyl groups of the polymers. A compound represented by General Formula (2) is bonded to at least some of the carboxyl groups, from among the carboxyl groups of the polymers, that are not bonded to the first target substance capturing molecules.03-05-2009
20100291710BIOSENSOR SYSTEM FOR EXTERNAL ACTUATION OF MAGNETIC PARTICLES IN A BIOSENSOR CARTRIDGE - The invention provides for a biosensor system (11-18-2010
20100291709HUMAN NT-PRO B-TYPE NATRIURETIC PEPTIDE ASSAY HAVING REDUCED CROSS-REACTIVITY WITH OTHER PEPTIDE FORMS - The present disclosure relates to assays for detecting and/or quantifying the amount of human NT-pro B-type natriuretic peptide or human NT-pro B-type natriuretic peptide fragment in a test sample.11-18-2010
20110124130DEVICE AND METHOD FOR ANALYSIS OF SAMPLES WITH DEPLETION OF ANALYTE CONTENT - A system and method for determining the presence and/or concentration of one or more analytes in a sample that comprises a fluid, the system comprising a substrate comprising a sample inlet or inlets and one or more analyte determination flow paths, each analyte determination flow path comprising a defined beginning and a defined terminus and comprising at least one capture zone containing a capture agent for an analyte, the capture agent or agents being immobilized along a portion of the flow path or paths, the flow path or paths being designed so that the one or more analytes are depleted from the sample and bound to the portion of the flow path or paths containing immobilized capture agent or agents, producing an analyte depletion end region for each analyte between the beginning and the terminus of the analyte determination flow path.05-26-2011
20110124129METHODS AND SYSTEMS TO CONTROL FLUID FLOW IN ACCORDANCE WITH A PREDETERMINED SEQUENCE - Methods and systems to perform sequential user-controlled fluidic assays, using substantially self-contained, portable, user-initiated fluidic assay systems, including user-initiated activation methods and systems.05-26-2011
20110124128Centrifugal Force Based Microfluidic System And Method For The Automated Analysis of Samples - Centrifugal force based microfluidic systems for the automated analysis of fluids involving the use of magnetically responsive particles and methods thereof are disclosed. A magnet is fixed to a supporting device of the system in correspondence to a retention zone of the system so as to rotate therewith and to generate a magnetic field which magnetically manipulates the magnetically responsive particles contained in a reaction chamber of the system.05-26-2011
20110124127METHODS OF QUANTIFICATION FOR LATERAL FLOW DEVICES - The invention concerns methods of quantification of an analyte, A, in a test sample by means of a single immunochromatographic device, such as a lateral flow device (LFD). One method comprises the steps of: a) mixing a determined amount of said test sample with a determined amount of a quantification agent, QA05-26-2011
20110003401Partial Derivatization of Particles - There is disclosed a method for partially derivatizing a curved surface of particles in an electrically conducting solvent, said method comprises the steps: a) bringing particles in close contact with at least one surface by using a force, b) inducing a chemical reaction on at least one part of a particle by applying an electrical potential between said at least one surface and the electrically conducting solvent, and c) further reacting said at least one part of a particle where a chemical reaction has been induced in step b) above. There is further disclosed a partially derivatized particle as well as uses of the particle. Advantages include that the method is simple and only requires a potentiostat in addition to standard laboratory equipment, is inexpensive, time-efficient, and inherently parallel.01-06-2011
20100203653Protein G-Oligonucleotide Conjugate - The present invention relates to a protein G conjugate, which is prepared by linking an N-terminal cysteine-tagged protein G variant with an oligonucleotide via a linker. The conjugate binds in a directional manner on the surface of a biochip and biosensor, thereby providing a biochip and biosensor having improved antibody immobilization ability.08-12-2010
20100203652Method and apparatus for selective capture of gas phase analytes using metal beta-diketonate polymers - A process and sensor device are disclosed that employ metal β-diketonate polymers to selectively capture gas-phase explosives and weaponized chemical agents in a sampling area or volume. The metal β-diketonate polymers can be applied to surfaces in various analytical formats for detection of: improvised explosive devices, unexploded ordinance, munitions hidden in cargo holds, explosives, and chemical weapons in public areas.08-12-2010
20100203651Methods and compounds for phototransfer - Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.08-12-2010
20110236999Hydrogel Nanoparticle Based Immunoassay - An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.09-29-2011
20110236998METHODS AND DEVICES FOR DETECTION OF ANALYTES USING BLOCH SURFACE WAVE-ENHANCED DIFFRACTION-BASED SENSORS - The invention features methods and diffraction-based devices for the detection of specific analytes. The devices of the invention contain a periodic dielectric multilayer, which allows for the propagation of Bloch surface waves (BSWs) at the surface of the multilayer, thereby increasing the sensitivity of the device.09-29-2011
20110263048INSOLUBLE CARRIER FOR USE IN ANTI-PHOSPHOLIPID ANTIBODY MEASUREMENT REAGENT, ANTI-PHOSPHOLIPID ANTIBODY MEASUREMENT REAGENT, AND METHOD FOR MEASURING ANTI-PHOSPHOLIPID ANTIBODY - The present invention has an object to provide an insoluble carrier for an antiphospholipid antibody detection reagent having a high reactivity. The present invention also has an object to provide an antiphospholipid antibody detection reagent, and a method of detecting an antiphospholipid antibody. The present invention directs to an insoluble carrier for an antiphospholipid antibody detection reagent, having a zeta potential of lower than −45 mV in the case that the insoluble carrier is suspended in a 20 mmol/L aqueous sodium phosphate solution with a pH of 7.4 so that the resulting suspension has a solids concentration of 0.1%.10-27-2011
20100167422 METHOD OF DETERMINING ANALYTE CONCENTRATION - A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: 07-01-2010
20080274565Method for the quantitative measurement of analytes in a liquid sample by immunochromatography - The invention concerns a method for the quantitative measurement of at least one analyte of interest in a liquid sample by immunochromatography, said method comprising weighted measurement of the quantity of analyte with respect to a control.11-06-2008
20080254551Immunoprecipitaion-Based Method to Purify and Characterise Biological Macromolecular Complexes - This invention concerns an artificial adapter protein that combines an antibody-binding activity with two affinity tags and its use in isolation of antibody-antigen complexes. Using this adapter protein, complexes can be obtained at good yield and in the high purity necessary for the identification of all biological macromolecules that are associated with the antigen.10-16-2008
20080248593METHOD FOR DETECTION OF FLUORIDE OR HYDROGEN FLUORIDE AND DETECTION KIT - The present invention relates to a method for detecting and/or measuring the concentration of fluoride (F10-09-2008
20080248592Detection of target analytes using particles and electrodes - The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.10-09-2008
20080248589Sample Presentation Device - The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.10-09-2008
20080233660Solid phase labeling method - The invention provides for the labeling of antibodies with a fluorescent label, using a solid support comprising an affinity for an Fc portion of an antibody. Thus, the invention provides a method for labeling an antibody or fragment thereof with a fluorescent label, comprising the steps of immobilizing an antibody on the solid support and covalently coupling a fluorescent label to the immobilized antibody, as well as a kit for performing such method.09-25-2008
20090068759REUSABLE DETECTION SURFACES AND METHODS OF USING SAME - The technology provided herein generally relates to reusable detection surfaces and methods for reusing a detection surface after using the detection surface in an assay for an analyte.03-12-2009
20110237000Method for detecting an analyte molecule - The invention relates to a method for detecting the presence or amount of an analyte, said method comprising (a) coupling the analyte to a carrier molecule, wherein the carrier molecule is larger in size, electrically charged and/or polar, to form an analyte:carrier molecule complex; (b) contacting the analyte:carrier molecule complex of (a) with an analyte-binding molecule coupled to a semiconducting nanostructure; and (c) determining the change in conductance upon binding of the analyte:carrier molecule complex to the analyte-binding molecule and correlating the determined change in conductance to the presence or amount of the analyte. Alternatively, the analyte:carrier molecule complex of (a) is immobilized on the nanostructure and the immobilized analyte:carrier molecule complex is contacted with the analyte-binding molecule.09-29-2011
20100285612FLOW RATE MEASUREMENT APPARATUS, ANTIGEN CONCENTRATION MEASUREMENT APPARATUS, FLOW CELL, FLOW RATE MEASUREMENT METHOD, AND ANTIGEN CONCENTRATION MEASURING METHOD - A flow rate measurement apparatus includes a light oscillator; a thin metallic film which causes surface plasmon resonance by light output from the light oscillator; a focusing unit which fixes the thin metallic film and converts the output light of the light oscillator into incident light having a plurality of incident angles to focus the incident light at a location of a focal line in a straight line shape on the thin metallic film; a measurement part having antibody fixed areas to which an antibody is fixed and reference areas to which an antibody is not fixed, the antibody fixed areas and the reference areas being alternately arranged at a location along the focal line location on the thin metallic film; a light receiver which receives reflected light, at the focal line location, of the output light by surface plasmon resonance occurring at the focal line location, at each of the plurality of incident light angles; an SPR angle calculator which obtains a temporal change of an SPR angle in each of the antibody fixed areas and the reference areas in the measurement part; and a flow rate operation unit which calculates the flow rate of the sample flowing in the flow cell based on the temporal change of the SPR angle obtained by the SPR angle calculator.11-11-2010
20090068760MICROFLUIDIC ASSAY SYSTEM WITH DISPERSION MONITORING - Disclosed is a microfluidic assay system and methods that apply flow injection analysis to permit dispersion monitoring. A solution containing a reagent that binds an analyte and a tracer is delivered via pressure-driven flow into the receiving end of the injection channel of the system of the invention. A sample fluid suspected of containing the analyte is delivered into the upstream end of the input channel under conditions permitting flow of the sample fluid toward the downstream end of the assay channel and permitting dispersion of the reagent into the sample fluid. The amount of tracer present in the fluid as it passes over the reference region and the capture region and the amount of binding between the analyte and the capture region are detected. The amount of binding detected between the analyte and the capture region is correlated to the amount of tracer detected in the reference region.03-12-2009
20090087926IMMUNOCHROMATOGRAPHIC TEST DEVICE - The invention provides an immunochromatographic test device for detecting a test substance in a sample, comprising a chromatographic membrane carrier; a sample developing member; and a labeling substance holding member; wherein a substance being capable of reacting to a human anti-mouse antibody is held in a member disposed at a position which is more upstream on the basis of the sample developing direction than the chromatographic membrane carrier, as well as an a method for detecting a test substance in a sample by using an immunochromatographic test device and a method for manufacturing an immunochromatographic test device.04-02-2009
20120015451FLUID SENSOR PREVENTING GENERATION OF AIR BUBBLES - Provided herein is a fluid sensor, which includes a closed reaction unit in which reaction of a fluid sample takes place. The reaction unit is tapered on a side through which the fluid is injected so as to prevent generation of air bubbles during the injection of the fluid. Thus, the sensor has improved sensitivity.01-19-2012
20120094397DETECTION METHOD AND DETECTION SYSTEM - In a detection method for detecting the quantity of a target material, a labeled binding material in the amount corresponding to the quantity of the target material contained in a liquid specimen is bonded to the top of a sensor portion; and a signal based on light emitted from a label in an evanescent field or an enhanced optical field produced on a surface of the sensor portion when the sensor portion is irradiated with excitation light is detected. After the labeled binding material is bonded to the immobilization layer, the signal is detected while the fluid over the sensor portion is controlled to flow at a constant flow rate at which bonds between the labeled binding material and the immobilization layer are not broken and the above signal can be detected with a greater magnitude than when the liquid specimen exists over the sensor portion at rest.04-19-2012

Patent applications in class INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS

Patent applications in all subclasses INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS