Class / Patent application number | Description | Number of patent applications / Date published |
435270000 | Removing nucleic acid from intact or disrupted cell | 33 |
20080213870 | Methods for obtaining modified DNA from a biological specimen - The present invention provides a method for obtaining modified DNA from a biological specimen by obtaining a cell suspension from the specimen, if necessary; passing the cell suspension through a first filter under conditions sufficient to obtain filter-bound cells and suspended DNA; lysing the filter-bound cells under conditions sufficient to release cellular DNA; modifying the DNA bound to the filter under conditions sufficient to release the modified DNA from the filter into a flow-through volume; passing the flow-through volume through a second filter under conditions sufficient to capture the modified DNA to the second filter; and eluting the modified DNA from the second filter. | 09-04-2008 |
20080233632 | Process for Labeling and Purification of Nucleic Acids of Interest Present in a Biological Sample to be Treated in a Single Reaction Vessel - The present invention relates to a process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
| 09-25-2008 |
20080248559 | Method For Selectively Separating and Purifying Rna and Method For Separating and Purifying Nucleic Acid - A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm | 10-09-2008 |
20080261292 | Method Enabling the Use of Extracellular Ribonucleic Acid (RNA) Extracted from Plasma or Serum to Detect, Monitor or Evaluate Cancer or Premalignant Conditions - This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)-RNA complexes, protein-RNA complexes including within or in association with ribonucleoprotein complexes, nucleosomes, or within apoptotic bodies. Any intracellular RNA found in plasma or serum can additionally be detected by this invention. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any neoplasm, benign, premalignant, or malignant, in humans or other animals, which might be associated with that RNA. Further, this invention allows the qualitative or quantitative detection of tumor-derived or associated extracellular RNA circulating in the plasma or serum of humans or animals with or without any prior knowledge of the presence of cancer or premalignant tissue. | 10-23-2008 |
20090068724 | Isolation of Nucleic Acid - The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be coupled with a further step to isolate RNA from the same sample. | 03-12-2009 |
20090137024 | Method of Separating Target DNA from Mixed DNA - The present invention relates to methods of separating target DNA from mixed DNA in a sample. In some embodiments, the target DNA may be viral DNA, bacterial DNA, fungal DNA or combinations thereof. In some embodiments the mixed DNA includes target DNA and non-target DNA. | 05-28-2009 |
20090162924 | COMPOSITIONS AND METHODS FOR OBTAINING NUCLEIC ACIDS FROM SPUTUM - The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention. | 06-25-2009 |
20090246859 | Methods and Compositions for Purification of Nucleic Acid from a Host Cell - Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 μm. | 10-01-2009 |
20090286304 | ENZYMATIC DIGESTION OF TISSUE - The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample. | 11-19-2009 |
20090286305 | Method for non-destructive macromolecule extraction from biological samples on slide - A thin layer of a biological sample, such as a section of frozen or preserved tissue sample, a section of fresh or preserved cells, and a mono layer of prokaryotic and eukaryotic cells, is placed on a flat surface of a solid supporting base. Macromolecules, such as DNA, RNA, and proteins, are extracted directly from the thin layer of the biological sample that is attached to the supporting base. | 11-19-2009 |
20090317894 | CELL LYSIS REAGENT FOR ISOLATION OF RNA - RNA is extracted from cellular material with a reagent that includes heparin, a reducing agent to reduce disulfide bonds, a chelating agent, a buffer, and an alkali metal halide. The reagent does not require the use of organic solvents, and the reagent allows extraction to be performed in a relatively short period of time in comparison to the prior art. | 12-24-2009 |
20090325272 | Nucleic Acid Sample Preparation by Exclusion of DNA - Devices and methods are provided for separation of nucleic acids from waste materials in a biological sample, and then reacting the separated nucleic acids. In some embodiments, the methods comprise mixing a cell lysate having nucleic acids and waste materials with a waste-binding matrix to capture the cellular waste materials from the lysate. The waste-binding matrix can comprise hydrophilic and/or hydrophobic size-exclusion, ion-exchange particles. In some embodiments, the device comprises a substrate comprising a fluid processing pathway in which nucleic acid sample preparation occurs prior to a downstream genotyping reaction. | 12-31-2009 |
20100035331 | METHOD FOR EXTRACTING OF NUCLEIC ACID FROM BIOLOGICAL MATERIAL - A method of extracting biological material-derived nucleic acid from samples containing biological material, the method comprising mixing biological material with an aqueous solution containing at least a surfactant, subjecting the mixture to heating treatment and then removing the aqueous solution to separate the biological material, adding a solid powder suspension to the biological material, agitating or sonicating it to disrupt the biological material, and extracting nucleic acid from the biological material. | 02-11-2010 |
20100062518 | Concentrating White Blood Cells for DNA Extraction from a Leukodepleted Blood Sample - Reagents and a method for the pre-concentration of WBCs from leukodepleted blood segment samples are described. The reagent comprises: a lytic reagent, for example, saponin in phosphate buffered saline(PBS), wherein the amount of saponin is in the range of from about 1% to about 10% percent (w/w). The method comprises:
| 03-11-2010 |
20100120129 | APPARATUS FOR AND METHOD OF PROCESSING BIOLOGICAL SAMPLES - The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like. | 05-13-2010 |
20100190240 | REAGENTS FOR NUCLEIC ACID PURIFICATION - Embodiments of the present invention provide methods and kits for purifying nucleic acids. In particular, embodiments of the present invention provide methods and kits for purifying nucleic acids through the use of magnetic particles in binding buffers. | 07-29-2010 |
20100216220 | Devices and Methods for the Purification, Isolation, Desalting or Buffer/Solvent Exchange of Substances - A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method. | 08-26-2010 |
20100248336 | METHODS FOR PURIFYING NUCLEIC ACIDS - Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, following by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided. | 09-30-2010 |
20110003369 | METHOD FOR THE DEPARAFFINISATION OF BIOLOGICAL SPECIMENS - A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact
| 01-06-2011 |
20110076751 | LYSIS, BINDING AND/OR WASH REAGENT FOR ISOLATING AND/OR PURIFYING NUCLEIC ACIDS - The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids. | 03-31-2011 |
20110195486 | METHODS FOR REMOVING NUCLEIC ACID CONTAMINATION FROM REAGENTS - In general, the disclosed method can be used to remove contaminating microbes and nucleic acids from microorganisms-derived reagents, apparatus and processes (materials and apparatus) related to PCR (and RT-PCR), including sample prep reagents and materials that are used to isolate, purify and detect nucleic acids. | 08-11-2011 |
20110300608 | NUCLEIC ACID ISOLATION IN PRESERVED WHOLE BLOOD - A method for isolating nucleic acids is disclosed, wherein a sample having nucleic acid containing starting material is fixed, lysed, and treated to remove unwanted contaminants. The initial fixing of the sample aids in maintaining the structure and integrity of the isolated DNA and reduces the incidence of end product contaminants and DNA shearing. | 12-08-2011 |
20120100599 | Nucleic Acid Isolation Using Polidocanol and Derivatives - This invention relates to a composition comprising a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof. The invention is further related to uses of this composition and to a kit comprising the composition according to the invention. The invention is further related to a method for the detection of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof, optionally isolating the nucleic acid, optionally amplifying the nucleic acid, and detecting the nucleic acid. The invention is further related to a method for the purification of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof and isolating the nucleic acid thereby purifying the nucleic acid. | 04-26-2012 |
20130052721 | METHOD FOR ISOLATING RNA FROM A RNA AND DNA CONTAINING SAMPLE - The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages. | 02-28-2013 |
20130164826 | MICROFLUIDIC APPARATUS, METHOD, AND APPLICATIONS - A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process. | 06-27-2013 |
20130273640 | NUCLEIC ACID SAMPLE PREPARATION - The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples. | 10-17-2013 |
20130288342 | POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA - The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. | 10-31-2013 |
20130295652 | SYSTEM AND METHOD FOR ISOLATION OF SAMPLES - Systems and methods for isolating samples are provided. The system comprises a first membrane and a second membrane disposed within an enclosure. First and second reservoirs can also be disposed within the enclosure and adapted to contain one or more reagents therein. A first valve can be disposed within the enclosure and in fluid communication with the first reservoir, the second reservoir, or both. The first valve can also be in fluid communication with the first or second membranes or both. The first valve can be adapted to selectively regulate the flow of the reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir. | 11-07-2013 |
20140087449 | Method for Providing DNA Fragments Derived from an Archived Sample - Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc.). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable. | 03-27-2014 |
20140162347 | BIDIRECTIONAL TRANSFER OF AN ALIQUOTE OF FLUID BETWEEN COMPARTMENTS - An apparatus adapted to extract an ingredient from a fluid sample by bidirectional transferring of an aliquot of fluid having magnetic particles therein, said apparatus comprising: an open compartment operable at a constant ambient pressure; a closed compartment having a closed pressure, and an upper zone containing an air pocket adapted to generate internal positive and negative pressure; an intermediate transferring-retaining compartment adapted to temporarily retain a fraction of said aliquot of fluid; a flow barrier member adapted to inhibit a flow of said aliquot of fluid when said closed pressure is substantially equal to said ambient pressure and to allow said flow of fluid when said closed pressure is substantially different from said ambient pressure, the pressure differential controllable by a thermo member; a capturing zone adapted to allow magnetic particles in the fluid to be attracted in response to a magnetic force applied. | 06-12-2014 |
20160017314 | METHOD FOR ISOLATING NUCLEIC ACIDS FROM FORMALIN-FIXED PARAFFIN EMBEDDED TISSUE SAMPLES - Methods are disclosed for isolating nucleic acids from formalin-fixed paraffin embedded (FFPE) tissue samples. Each of tissue samples contains paraffin and a target biological tissue or material, and the method includes the steps of: adding a first reagent and a second reagent to the FFPE tissue sample, the first reagent dissolving the paraffin material and the second reagent lysing the biological tissue; mixing the first reagent, the second reagent, and the FFPE tissue sample to form a first mixture; (2) heating the first mixture at 50-80° C. for 30-90 minutes; and then heating the first mixture at 80-95° C. for 30-90 minutes to fractionize the first mixture to form an aqueous phase and an oil phase; (3) collecting an aqueous solution from the aqueous phase; and (4) isolating nucleic acids from the aqueous solution. The method improves the efficiency and convenience of isolating nucleic acids from FFPE tissue samples. | 01-21-2016 |
20160017316 | ISOLATION OF MEGABASE-SIZED DNA FROM PLANT AND ANIMAL TISSUES - Disclosed herein are methods for isolation of long DNA molecules, for example megabase-sized genomic DNA molecules, from a biological sample, for example plant and animal tissues. | 01-21-2016 |
20160186167 | Method and Device for Processing a Sample of Biological Material Containing Target Cells and Companion Cells in Order to Extract Nucleic Acids of the Target Cells - A method for processing a sample of biological material containing target cells and companion cells in order to extract nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the companion cells from the sample. The method additionally includes decomposing the target cells by chemical or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method further includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acid of the target cells. | 06-30-2016 |