Class / Patent application number | Description | Number of patent applications / Date published |
435261000 | Separation of micro-organism from culture media | 13 |
20080213869 | Hydrogel for separating cell and method of separating cell - Cell/organism is separated by using a hydrogel capable of selectively moving a cell in accordance with a difference in the concentration of a physiologically active substance. The invention provides a hydrogel capable of conducting separation (fractionation, differential separation, fractional collection) of cell/organism having a variety of taxes, which could not be attained in the prior art. | 09-04-2008 |
20090004727 | METHOD FOR THE SEPARATION OF MICROORGANISMS ADHERED TO A SOLID SAMPLE BY MEANS OF A PHOSPHATE-BASED DAMPER AND SONICATION OF THE SAMPLE - The present invention provides a method for the separation of microorganisms of a microbiological community present in a solid sample. And once the microorganisms are isolated, they are used in microbiological techniques, or their nucleic acids, both DNA and RNA, are extracted from them for carrying out molecular biology techniques. In accordance with the present invention, the method for the separation of microorganisms adhered to a solid sample comprises the following stages: Providing a solid sample with microorganisms adhered to it; adding to the solid sample a high molarity phosphate-based buffer containing a phosphate solution of a concentration greater than 0.5 M, ethanol, and a non-ionic surfactant; sonicating, and recovering the supernatant, comprising microorganisms separated from the solid sample. | 01-01-2009 |
20090162920 | MODULAR CONTINUOUS PRODUCTION OF MICRO-ORGANISMS - Process for the growing of micro-organism in an open and continuous system further being characterized by means for creating within said system a habitat in which natural, diverse and heterogeneous emicrobial communities can autonomously react and adapt to a changing environment | 06-25-2009 |
20090191613 | Aerobic Compost Tea Making Device and Method - The device and method invented provides a unique means to extract and multiply, by the millions, beneficial aerobic microbes found in compost and vermicompost to be applied to soil and plants in liquid form. This liquid has been named compost tea colloquially. Compared to many compost tea making devices being sold, the invented device is truly simple and can be dismantled and cleaned in under twenty minutes. Most of the parts are not glued and can be pulled apart. The device and method uses air pumps alone to actually circulate the water and can be used with or without a mesh extractor. This is achieved by the insertion of an air diffuser into the piping used, which infuses the water with oxygen while circulating the water, into either, an extractor containing compost or a body of water containing compost. Concurrently additional diffusers infuse the body of water with oxygen. | 07-30-2009 |
20090258411 | CENTRIFUGAL DEVICE AND METHOD FOR OVA DETECTION - A centrifugal device and method are provided for the separation of buoyant material such as parasitic ova from fecal matter. A rotor assembly, rotatable about its central axis in a centrifuge, includes a housing with a centrally located top opening leading to a centrally located mixing chamber. An annular sediment chamber is provided, also coaxial about the central axis, connected by a passage with the mixing chamber. A coring assembly is used to retrieve and insert a fecal sample into the mixing chamber for mixing with a flotation fluid. During centrifugation, heavier fecal components pass radially outwardly to the sediment chamber while the ova collect on the inward surface of the flotation fluid. After centrifugation, more flotation fluid is added, if needed, until a meniscus forms at the top opening. A coverslip is placed over the top opening and the ova float to the surface of the fluid and adhere to the coverslip. The coverslip is removed and the ova detected using standard microscopy procedures. In another aspect, a centrifugal device is provided in which the ova are delivered through centrifugation to a pipette tip for dispensing onto a microscope slide or coverslip. | 10-15-2009 |
20100120127 | METHOD FOR REMOVING BACTERIA FROM A FERMENTATION BROTH - The invention relates to method for the stepwise removal of bacteria from a fermentation broth. According to said method, the bacteria in the fermentation broth are mixed with a retention promoter mixture in a first process step and the pretreated bacteria are removed by precoat filtration in a second process step. The inventive method uses a retention promoter mixture from bentonite and polycations such as PEI or polyDADMAC and is suitable for treating large quantities of bacteria. The filter cake obtained with bentonite/PEI has a substantially improved quality. | 05-13-2010 |
20100227379 | Compact disk based system for separating immunomagnetic bead labeledparticulates and method thereof - Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber. Particulates not labeled with the immunomagnetic beads and contained in the sample fluid move with the sample fluid to the outer reservoir. | 09-09-2010 |
20120238003 | MICROORGANISM CONCENTRATING APPARATUS AND METHOD - A microorganism-concentrating apparatus according to one embodiment includes a mixing device, a filtering device, and a magnetic separation device. The mixing device is configured to mix an aqueous suspension of microorganisms with a particulate filter aid includes magnetic particles to prepare an aqueous slurry of the microorganisms and the particulate filter aid. The filtering device is configured to filter the aqueous slurry supplied from the mixing device to provide a filter cake includes the microorganisms and the particulate filter aid, and to provide a filtrate. The magnetic separation device is configured to magnetically separate the filter cake supplied from the filtering device into the microorganisms and the particulate filter aid. | 09-20-2012 |
20130089917 | Single Use Centrifuge System for Highly Concentrated and/or Turbid Feeds - A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates. | 04-11-2013 |
20130109079 | METHOD FOR SEPARATING AN ORGANIC COMPONENT FROM A MIXTURE CONTAINING THE ORGANIC COMPONENT | 05-02-2013 |
20140099700 | APPARATUS AND SYSTEM FOR BIOREACTOR CULTIVATING MICROORGANISMS AND REDUCING CARBON DIOXIDE - A bioreactor includes a support structure and a translucent liner. A containing system of bioreactors includes a plurality of bioreactors. A contained bioreactor system includes a plurality of containing systems of interconnected bioreactors, an industrial blower, an air filter, a microbial filter, a gas delivery system, and a harvesting station. A method of reducing carbon dioxide emissions includes delivering carbon dioxide to a bioreactor where the bioreactor includes a support structure and a liner where the bioreactor includes media containing microbes. A method of harvesting microorganisms includes emptying the solution and removing a sieve from the harvesting unit. | 04-10-2014 |
20140273176 | Systems and Methods for Utilizing and Recovering Chitosan to Process Biological Material - Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material. | 09-18-2014 |
20140335600 | CELL COLLECTION METHOD - A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, the method including transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container. | 11-13-2014 |