Class / Patent application number | Description | Number of patent applications / Date published |
435254200 | Yeast; media therefor | 78 |
20090081764 | Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay - The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein αβγ complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric Gα protein is expressed in conjunction with nucleotide sequences from the yeast G protein βγ subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using such expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems. | 03-26-2009 |
20090123991 | RETRONS FOR GENE TARGETING - The invention provides methods and nucleic acid constructs that may be used to modify a nucleic acid of interest at a target locus within the genome of a host. In some aspects, the invention contemplates producing in vivo a gene targeting substrate (GTS), which may be comprised of both DNA and RNA components. The gene targeting substrate may comprise a gene targeting nucleotide sequence (GTNS), which is homologous to the target locus, but comprises a sequence modification compared to the target locus. The gene targeting substrate may be produced by reverse transcription of a gene targeting message RNA (gtmRNA). The gene targeting message RNA may be folded for self-priming for reverse transcription by a reverse transcriptase. The gene targeting message RNA may in turn be the product of transcription of a gene targeting construct (GTC) encoding the gene targeting message RNA. The gene targeting construct may for example be a DNA sequence integrated into the genome of the host, or integrated into an extrachromosomal element. Following expression of the gene targeting systems of the invention, hosts may for example be selected having genomic modifications at a target locus that correspond to the sequence modification present on the gene targeting nucleotide sequence. In some embodiments, the structure of retrons may be adapted for use in the gene targeting systems of the invention. | 05-14-2009 |
20090233346 | PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS - The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of | 09-17-2009 |
20090233347 | PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS - The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of | 09-17-2009 |
20100035329 | Production of Isoprenoids - The invention provides a biological method of producing isoprenoids. | 02-11-2010 |
20100068789 | AMMONIUM TRANSPORTER PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST - The promoter region associated with the | 03-18-2010 |
20100075402 | DNA coding for polypeptide fusion - A hybrid protein includes two coexpressed amino acid sequences forming a dimer. Each sequence contains the binding portion of a receptor, such as TBP1 or TBP2, or a ligand, such as IL-6, IFN-β and TPO, linked to a subunit of a natural heterodimeric scaffold. Each coexpressed sequence contains a corresponding subunit so as to form a heterodimer upon expression. Corresponding DNA molecules, expression vectors and host cells are also disclosed as are pharmaceutical compositions and a method of producing such proteins. | 03-25-2010 |
20100297738 | EXPRESSION SYSTEM - The present invention relates to methods for increasing the secretion of a protein of interest (POI) from a eukaryotic cell comprising co-expression of a POI and of at least one protein that enhances protein secretion, said enhancing protein being selected from the group consisting of BMH2, BFR2, C0G6, C0Y1, CUP5, IMH 1, KIN2, SEC31, SSA4 and SSE1. The invention further relates to a yeast promoter sequence, in particular to a promoter sequence of the PET9 gene of | 11-25-2010 |
20100304467 | METHODS FOR PRODUCING HUMAN CERAMIDE USING YEAST TRANSFORMANTS - The present invention provides a method for producing human ceramide in a yeast cell. | 12-02-2010 |
20110027867 | Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs - This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in viva | 02-03-2011 |
20120129241 | AN L-ARABINOSE FERMENTING YEAST - An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains. | 05-24-2012 |
20120142082 | CAROTENOID PRODUCTION IN A RECOMBINANT OLEAGINOUS YEAST - Engineered strains of the oleaginous yeast | 06-07-2012 |
20130089914 | METHOD FOR GENERATING A GENETICALLY MODIFIED MICROBE - Provided herein are methods of generating genetically modified microorganisms, e.g., genetically modified yeast strains, which comprise functional disruptions in one or more pheromone response genes and one or more sporulation genes, and genetically modified yeast cells, e.g., genetically modified diploid and haploid yeast cells, that lack sporulation capability and endogenous mating capability, produced thereby. | 04-11-2013 |
20140030795 | MODIFIED YEAST STRAINS EXHIBITING ENHANCED FERMENTATION OF LIGNOCELLULOSIC HYDROLYSATES - The present invention relates to novel xylose-fermenting yeast strains (for example, yeast of the genus | 01-30-2014 |
20140212953 | YEAST MICROORGANISMS WITH REDUCED BY-PRODUCT ACCUMULATION FOR IMPROVED PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 07-31-2014 |
20140242674 | PENTOSE FERMENTATION BY A RECOMBINANT MICROORGANISM - The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods. | 08-28-2014 |
20140248689 | GLUCOSE-INDUCED INACTIVATION/DEGRADATION-RESISTANT TRANSPORTER GENE AND USE THEREOF - The present invention relates to a glucose-induced inactivation/degradation-resistant transporter gene and use thereof, and more particularly, to a brewing yeast having excellent assimilation of oligosaccharides (maltose, maltotriose, etc.), an alcoholic beverage prepared using the yeast, a method of producing the alcoholic beverage, etc. More specifically, the present invention relates to a glucose-induced inactivation/degradation-resistant transporter including Mal21p, mutant Mal31p, mutant Mal61p, mutant Mtt1p, mutant Agt1p, etc., a gene encoding the transporter, a method of producing an alcoholic beverage using thereof, and so on. | 09-04-2014 |
20150140641 | ENDOGLUCANASE VARIANTS - The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type | 05-21-2015 |
20160010132 | PENTOSE FERMENTATION BY A RECOMBINANT MICROORGANISM | 01-14-2016 |
20160068850 | YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE - The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock. | 03-10-2016 |
20160152993 | Promoters for Expressing Genes in a Fungal cell | 06-02-2016 |
20160177283 | HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST | 06-23-2016 |
435254210 | Saccharomyces | 45 |
20080220503 | Screening method for genes of brewing yeast - Provided herein are a method for selecting a gene participating in the desire brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of | 09-11-2008 |
20090042277 | NUCLEIC ACIDS ENCODING RECOMBINANT SOLUBLE ENDOPROTEASES - The present invention relates to the expression and secretion in | 02-12-2009 |
20090042278 | Method of protein production in yeast - Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism. | 02-12-2009 |
20090053797 | GENETICALLY MODIFIED HOST CELLS AND USE OF SAME FOR PRODUCING ISOPRENOID COMPOUNDS - The present invention provides genetically modified eukaryotic host cells exhibiting increased activity levels of one or more enzymes that generate precursors to be utilized by the mevalonate pathway enzymes, increased activity levels of one or more mevalonate pathway enzymes, prenyl transferase, and/or decreased levels of squalene synthase activity; such cells are useful for producing isoprenoid compounds. The present invention provides genetically modified eukaryotic host cells that produce higher levels of acetyl-CoA than a control cell; such cells are useful for producing a variety of products, including isoprenoid compounds. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound. | 02-26-2009 |
20090209024 | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes - The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man | 08-20-2009 |
20090246857 | STABLE RECOMBINANT YEASTS FOR FERMENTING XYLOSE TO ETHANOL - Described are recombinant yeast which ferment xylose to ethanol and which maintain their ability to do so when cultured for numerous generations in non-selective media. The preferred yeast contain multiple copies of integrated genes encoding xylose reductase, xylitol dehydrogenase, and xylulokinase fused to promoters which are non-glucose inhibited and which do not require xylose for induction. Also described are preferred methods for integrating multiple copies of exogenous DNA into host cells by transforming cells with replicative/integrative vectors, and then replicating the cells a number of times under selective pressure to promote retention of the vector in subsequent generations. The replicated vectors thus serve to integrate multiple copies of the exogenous DNA into the host cells throughout the replication/selection phase. Thereafter the selective pressure can be removed to promote loss of the vector in subsequent generations, leaving stable integrants of the exogenous DNA. | 10-01-2009 |
20090311771 | ARABINOSE-AND XYLOSE-FERMENTING SACCHAROMYCES CEREVISIAE STRAINS - The present invention relates to a | 12-17-2009 |
20100021991 | Methods for Producing Modified Glycoproteins - Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man | 01-28-2010 |
20100028975 | TRAITS IN RECOMBINANT XYLOSE-GROWING SACCHAROMYCES CEREVISIAE STRAINS USING GENOME-WIDE TRANSCRIPTION ANALYSIS - New xylose-utilizing | 02-04-2010 |
20100203617 | USE OF SACCHAROMYCES CEREVISIAE ERG4 MUTANTS FOR EXPRESSING MAMMALIAN GLUCOSE TRANSPORTERS - The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains. | 08-12-2010 |
20100317087 | MODIFIED CELLULASES WITH INCREASED THERMOSTABILITY, THERMOPHILICITY, AND ALKALOPHILICITY - A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme. | 12-16-2010 |
20110020912 | METHOD FOR THE CONSTRUCTION OF RANDOMIZED GENE SEQUENCE LIBRARIES IN CELLS - An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a | 01-27-2011 |
20110039327 | ORGANIC ACID PRODUCTION BY FUNGAL CELLS - Improved systems for the biological production of organic acids are described. | 02-17-2011 |
20110059514 | Yeast Biocatalysts for Degradation of Biowarfare Agents - The present disclosure relates to yeast biocatalysts and methods of using yeast biocatalysts for detoxifying a biowarfare agent. In some embodiments, a yeast biocatalyst may include a nucleic acid encoding a phage lysin operably linked to a prepro leader sequence, an expression control sequence operably linked to the nucleic acid, and a bioeffective amount (e.g., enough to detoxify) of the phage lysin. A yeast biocatalyst may include an engineered strain of a | 03-10-2011 |
20110059515 | PRODUCTION OF ISOPRENOIDS - The invention provides a biological method of producing isoprenoids. | 03-10-2011 |
20110059516 | PRODUCTION OF ISOPRENOIDS - The invention provides a biological method of producing isoprenoids. | 03-10-2011 |
20110165660 | ENGINEERED MICROORGANISMS WITH ENHANCED FERMENTATION ACTIVITY - Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms. | 07-07-2011 |
20110165661 | ENGINEERED MICROORGANISMS WITH ENHANCED FERMENTATION ACTIVITY - Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms. | 07-07-2011 |
20110229959 | ENGINEERED MICROORGANISMS WITH ENHANCED FERMENTATION ACTIVITY - Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms. | 09-22-2011 |
20120064607 | FERMENTATION ON PENTOSE SUGARS - The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi. | 03-15-2012 |
20120088290 | ETHANOL YIELD AND REDUCTION OF BIOMASS ACCUMULATION IN THE RECOMBINANT STRAIN OF SACCHAROMYCES CEREVISIAE OVEREXPRESSING ATPASE - A new approach for increase of ethanol yield during alcoholic fermentation via decrease of biomass accumulation by using ATP degrading enzymes is described. The part of the | 04-12-2012 |
20120122191 | Methods For Producing Secreted Polypeptides - The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides. | 05-17-2012 |
20120184020 | ENGINEERED MICROORGANISMS WITH ENHANCED FERMENTATION ACTIVITY - Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms. | 07-19-2012 |
20130011909 | METHODS AND COMPOSITION TO ENHANCE PRODUCTION OF FULLY FUNCTIONAL P-GLYCOPROTEIN IN PICHIA PASTORIS - The present invention provides codon optimization to increase protein production by providing a target gene, wherein the expression of the target gene is to be optimized; determining one or more low-frequency codons in the target gene; providing a codon usage frequency table; replacing each of the one or more low-frequency codons in the target gene with a corresponding high-frequency codons that code for the same amino acid; and harmonizing the a distribution of codon frequencies to those of the set of highly expressed native gene over an open reading frame in the target gene to form an optimized gene, wherein the optimized gene encodes an amino acid sequence identical to the respective wild-type (native) amino acid sequence. | 01-10-2013 |
20130071908 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 03-21-2013 |
20130217098 | PRODUCTION OF ISOPRENOIDS - A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid. | 08-22-2013 |
20130267012 | Host Cells and Methods for Producing Diacid Compounds - The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism. | 10-10-2013 |
20130323822 | Yeast Expressing Saccharolytic Enzymes for Consolidated Bioprocessing Using Starch and Cellulose - The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock. | 12-05-2013 |
20140017768 | XYLOSE ISOMERASE AND XYLITOL DEHYDROGENASE COMBINATION FOR XYLOSE FERMENTATION TO ETHANOL AND B. fragilis XYLOSE ISOMERASE - Disclosed herein is a newly discovered problem and solution for engineering | 01-16-2014 |
20140038268 | Activity of Fe-S Cluster Requiring Proteins - The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. | 02-06-2014 |
20140186930 | Methods for the Improvement of Product Yield and Production in a Microorganism Through the Addition of Alternate Electron Acceptors - The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more heterologous enzymes that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated. | 07-03-2014 |
20140206070 | Vector With Codon-Optimised Genes for an Arabinose Metabolic Pathway for Arabinose Conversion in Yeast for Ethanol Production - The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimised nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example. | 07-24-2014 |
20140273166 | PROPAGATING AN ORGANISM AND RELATED METHODS AND COMPOSITIONS - The present invention is related to methods of propagating one or more organisms by using a carbon source that includes xylose (e.g., xylose syrup from pretreating lignocellulosic feedstock) and/or a nutrient source that includes a stillage component (e.g., thin stillage derived from a corn-to-ethanol process) in the propagation medium. The organisms include those that can convert one or more monosaccharides into an alcohol via fermentation, such as yeast. The present invention is also directed to related compositions. | 09-18-2014 |
20140273167 | SYSTEMS AND METHODS FOR YEAST PROPAGATION - The present invention relates to systems and methods for propagating yeast using a feedstock material such as starch and one or more enzymes (e.g., an enzyme cocktail) to break down the feedstock material into one or more monosaccharides at a sufficient rate so that the yeast can use the monosaccharides as a carbon source for producing more yeast cells while not producing an undue amount of alcohol. | 09-18-2014 |
20140308735 | INCREASED PRODUCTION OF ISOBUTANOL IN YEAST WITH REDUCED MITOCHONDRIAL AMINO ACID BIOSYNTHESIS - Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase. | 10-16-2014 |
20140315279 | PRODUCTION OF QUINONE DERIVED COMPOUNDS IN OLEAGINOUS YEAST AND FUNGI - The present invention provides systems for producing engineered oleaginous yeast or fungi that express quinone derived compounds. | 10-23-2014 |
20140370573 | METHOD FOR PRODUCING ISOPROPANOL AND RECOMBINANT YEAST CAPABLE OF PRODUCING ISOPROPANOL - Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced. | 12-18-2014 |
20150064772 | NON-RECOMBINANT SACCHAROMYCES STRAINS THAT GROW ON XYLOSE - The present invention relates to methods for producing | 03-05-2015 |
20150344532 | ENGINEERED XYLOSE TRANSPORTERS WITH REDUCED GLUCOSE INHIBITION - Provided herein are compositions and methods useful for reducing glucose inhibition in transporting xylose, arabinose and other monosaccharides, into a yeast cell. | 12-03-2015 |
20150368656 | INCREASED PRODUCTION OF ISOBUTANOL IN YEAST WITH REDUCED MITOCHONDRIAL AMINO ACID BIOSYNTHESIS - Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase. | 12-24-2015 |
20160010117 | YEAST EXPRESSING CELLULASES FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CELLULOSE | 01-14-2016 |
20160040139 | PENTOSE FERMENTATION BY A RECOMBINANT MICROORGANISM - The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials. | 02-11-2016 |
20160053245 | Polypeptides Having Glucoamylase Activity And Polynucleotides Encoding Same - The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 02-25-2016 |
20160177317 | COMPOSITIONS AND METHODS FOR 3-HYDROXYPROPIONIC ACID PRODUCTION | 06-23-2016 |
20160251663 | ENGINEERED YEAST FOR PRODUCTION OF ENZYMES | 09-01-2016 |
435254220 | Candida | 6 |
20090035842 | Sucrose Feedstock Utilization for Oil-Based Fuel Manufacturing - The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar beets, and cane/beet agricultural processing byproducts as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a conversion technology to convert chemical energy produced by sugar cane and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oil-bearing microbes containing one or more exogenous sucrose utilization genes. | 02-05-2009 |
20120077252 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID - The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production. | 03-29-2012 |
20120156761 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID - The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production. | 06-21-2012 |
20130143305 | PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS - The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of | 06-06-2013 |
20130157343 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID - The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production. | 06-20-2013 |
20140004598 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID | 01-02-2014 |
435254230 | Pichia | 5 |
20090004725 | Process For Producing Protein With Reduction of Acidic Sugar Chain and Glycoprotein Produced Thereby - The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus | 01-01-2009 |
20100062516 | Expression of Class 2 Mannosidase and Class III Mannosidase in Lower Eukaryotic Cells - A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway. | 03-11-2010 |
20110183402 | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy - Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including | 07-28-2011 |
20140206071 | METABOLICALLY ENGINEERED YEASTS FOR THE PRODUCTION OF ETHANOL AND OTHER PRODUCTS FROM XYLOSE AND CELLOBIOSE - The present invention provides yeast cells that produce high concentrations of ethanol, culture media and bioreactors comprising the yeast cells, and methods for making and using the yeast cells in efficiently producing ethanol. | 07-24-2014 |
20160068849 | METHODS OF SYNTHESIZING HETEROMULTIMERIC POLYPEPTIDES IN YEAST USING A HAPLOID MATING STRATEGY - Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein. | 03-10-2016 |