Class / Patent application number | Description | Number of patent applications / Date published |
435397000 | Support is a gel surface | 37 |
20080248571 | Method of Production of Artificial Skin - A method for production of artificial skin by administering matrix metalloproteinase inhibitor or matrix metalloproteinase inhibitor and matrix protein production promoting agent. The matrix metalloproteinase inhibitor is N-hydroxy-2(R)-[[(4-methoxyphenyl)sulfonyl](3-picolyl)amino]-3-methylbutanamide hydrochloride. | 10-09-2008 |
20090068739 | Cell culture scaffold, methods of manufacturing the scaffold and cell culture methods - A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing a synthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS). | 03-12-2009 |
20090117655 | Culture Matrix for Forming a Cell Spheroid, and Method of Culturing the Same - A culture method for allowing only cancer cells to grow from a cancerous organ or tissue sample removed surgically or endoscopically, forming a spheroid of cancer cells having a tissue structure and different cell stages similar to a tissue structure and cell stages in the living body, and removing the cultured spheroid of cancer cells from the matrix by changing the temperature within the physiological temperature range. | 05-07-2009 |
20090117656 | STIMULI-RESPONSIVE DEGRADABLE GEL - To provide a new reductive-stimuli-responsive degradable gel that allows any control of decomposition of the three-dimensional base material for cell culture and production of a completely biological three-dimensional cellular structure consisting only of cells and cells-produced extracellular matrix and that allows safe recovery of the cellular structure produced. | 05-07-2009 |
20090155908 | BIOREACTOR FOR CELL GROWTH AND ASSOCIATED METHODS - Apparatuses, systems, and methods are provided for growing and maintaining cells. A three-dimensional matrix, such as a hydrogel material, is seeded with cells and placed in a bioreactor having two compartments. The matrix is supported between the two compartments by first and second porous materials, which engage opposing surfaces of the matrix. A first media stream having certain properties is propagated through the first compartment, where it contacts one surface of the matrix via the first porous material. A second media stream having different properties is propagated through the second compartment such that it contacts the opposite surface of the matrix via the second porous material. Through migration of each stream at least partially into the matrix, various controlled gradients may be established within the matrix, encouraging growth of the cells. Such gradients include osmotic pressure, oscillating osmotic pressure, hydrostatic pressure, oxygen tension, and/or nutrient gradients. | 06-18-2009 |
20100190255 | CROSS-LINKED GUMS FOR HEPATOCYTE CULTURE - This disclosure relates to cell culture surfaces derived from or contain gums including naturally occurring gums, plant gums, galactomannan gums or derivatives thereof including carboxyalkyl guar gum. Even more particularly, the disclosure relates to chemically or physically cross-linked modified gums where the gum surfaces are tuned to provide cell culture surfaces with physical and chemical characteristics particularly suited for hepatocyte culture. The disclosure also relates to articles of manufacture (e.g., cell culture vessels and labware) having such matrices, methods of making and providing the matrices to cell culture surfaces, and methods of using cell culture vessels having such matrices. | 07-29-2010 |
20100216243 | METAL ALGINATE HYDROGEL AND ARTICLES THEREOF - The present invention provides novel metal alginates prepared from non-cross-linked alginate monomers such as sodium alginate and an aqueous solution of a group 4, 5, or 6 metal oxyhalide. The novel group 4, 5, and 6 metal alginates are useful in the preparation of cell culture supports, exhibit useful X-ray contrast properties, and exhibit unanticipated stability to standard autoclave conditions relative to known metal alginates such as calcium alginate. In general, the novel metal alginates provided by the present invention offer features and properties not observed in known metal alginates such as calcium or barium alginates. | 08-26-2010 |
20100273262 | Apparatuses and methods for gel molding and culture - A gel molding apparatus is adapted to be used in combination with a vessel that has a plurality of solution chambers, and includes a lid plate and a plurality of columns that project from a top surface of the lid plate. Each of the columns has a bottom side connected to the top surface of the lid plate, a top side opposite to the bottom side, and a well recessed from the top side for receiving a gel suspension and having a depth from the top side. A method for gel molding is conducted via the gel molding apparatus. A culture apparatus includes the vessel and the gel molding apparatus. A method for culture is performed through the culture apparatus. | 10-28-2010 |
20110027888 | Coated Fibers for Culturing Cells - A coated fiber for cell culture includes a fiber core having an exterior surface and a polymeric coating suitable for culturing cells disposed on at least a portion of the exterior surface of the fiber core. A polypeptide may be conjugated to the polymeric coating. A method for forming the coated fiber includes coating a polymer layer to an exterior surface of a fiber core to produce the coated fiber. The coating may occur as the fiber is being drawn. | 02-03-2011 |
20110053269 | LAMINAR CONSTRUCT FOR TISSUE-ENGINEERED DERMAL EQUIVALENT - Compositions and methods for creating a laminar construct for tissue-engineered dermal equivalent are provided. One composition provided herein comprises a hydrogel matrix comprising two or more hydrogels layers and a population of stem cells. Associated methods are also provided. | 03-03-2011 |
20110201117 | FORMING POROUS SCAFFOLD FROM CELLULOSE DERIVATIVES - Scaffold comprises a polymer defining macropores and comprising hydroxypropylcellulose partially substituted by a substituent comprising a self-crosslinkable group, which is crosslinked through the self-crosslinkable group. The macropores have an average pore size larger than 50 microns and are at least partially interconnected. In one method, bicontinuous emulsion comprising a continuous aqueous phase and a continuous polymer phase is formed. The polymer phase comprises hydroxypropylcellulose partially substituted by a substituent comprising a self-crosslinkable group, and is crosslinked through the self-crosslinkable group to form a polymer defining at least partially interconnected pores. In another method, phase separation is induced in a solution comprising a polymer precursor and water to form a bicontinuous emulsion comprising a continuous polymer phase and a continuous aqueous phase. The polymer precursor comprises a self-crosslinkable group and is crosslinked through the self-crosslinkable group in the emulsion to form a polymer defining at least partially interconnected macropores. | 08-18-2011 |
20120208276 | TISSUE ENGINEERING SUPPORTS AND METHODS THEREFOR - The present disclosure relates to supports and scaffolds for cell and tissue engineering. The supports disclosed herein are composed of a thermally responsive material, containing pillars, that is coated with an acrylic polymer, thereby imparting an amphipathic matrix foundation. When exposed to a change in temperature, the coated support reacts by facilitating or repelling hydromolecular interactions. Further disclosed herein are methods for making hydrogels that can support tissue growth. | 08-16-2012 |
20120214238 | MICROBUBBLE DEVICES, METHODS AND SYSTEMS - A hydrogel tissue engineering scaffold having microbubbles dispersed therein is disclosed. Also, a system for cell culturing including a controller and actuator to apply dynamic deformational loading to a hydrogel is disclosed. Also disclosed are methods for producing hydrogels with microbubbles and for culturing cells using hydrogels with microbubbles. | 08-23-2012 |
20120231544 | CELL GROWTH APPARATUS AND USE OF AEROGELS FOR DIRECTED CELL GROWTH - This invention describes a cell growth apparatus, particularly neuronal printed circuit board apparatus comprising an aerogel base and a pre-printed cellular growth pattern. The cellular growth pattern is comprised of combinations of layers of cellular adhesion promoting materials, cellular adhesion inhibiting materials, and/or cellular signal promoting materials. The invention further describes methods of promoting cell growth using the neuronal printed circuit board apparatus of the invention. The invention is useful for regeneration and precise guidance of cells, particularly nerve cells, when used as an implant. | 09-13-2012 |
20120329156 | THREE-DIMENSIONAL SCAFFOLD AND METHOD OF MANUFACTURING THE SAME - A scaffold having a reinforced tissue regeneration ability and a method of manufacturing the same are provided. The scaffold is formed in a lattice form by alternately stacking biodegradable synthetic polymer-hydrogel layers. In this case, the biodegradable synthetic polymer-hydrogel layer is formed by disposing a plurality of biodegradable synthetic polymer-hydrogel units including a biodegradable synthetic polymer and a hydrogel at a predetermined gap. | 12-27-2012 |
20120329157 | CELL CONSTRUCT COMPRISING POLYMER BLOCKS HAVING BIOCOMPATIBILITY AND CELLS - It is an object of the present invention to provide a cell three-dimensional construct that has a thickness sufficient for tissue regeneration and comprises cells uniformly distributed therein. The present invention provides a cell construct comprising polymer blocks having biocompatibility and cells, wherein the plural polymer blocks are arranged in spaces between the plural cells. | 12-27-2012 |
20130130384 | TEMPERATURE RESPONSIVE SHEET THAT DISPLAYS REVERSIBLE PROPERTIES AND CELL SHEET PRODUCTION METHOD USING SAME - A temperature responsive sheet is disclosed that comprises a chemically modified water-soluble elastin obtained by N-acylating at least some of the primary amines and secondary amines contained in a high molecular weight water-soluble elastin molecule and coupling some or all of carboxyl groups contained in the molecule with a glycine alkyl ester. Also is disclosed a process for producing a cell sheet that comprises preparing above the temperature responsive sheet a film that functions as a scaffold for animal cells, culturing specific cells on the film so as to prepare a cell sheet, and subsequently separating the cell sheet and the temperature responsive sheet comprising the chemically modified water-soluble elastin under conditions of no greater than the culturing temperature for the cells. | 05-23-2013 |
20130149782 | Thermally Induced Gelation Of Collagen Hydrogel And Method Of Thermally Inducing Gelling A Collagen Hydrogel - The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels. | 06-13-2013 |
20130217126 | DRIED HYDROGEL, DRIED VITRIGEL MEMBRANE, AND METHODS FOR PRODUCING THE SAME - A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane. | 08-22-2013 |
20130217127 | CRYOGELS OF PVA-BORONIC ACID CONTAINING CO-POLYMERS FOR CELL CULTURE - A cryogel contains a polyol and a co-polymer of Formula (I). | 08-22-2013 |
20130236971 | HYDROGEL SCAFFOLDS FOR TISSUE ENGINEERING - Disclosed herein are biodegradable hydrogel scaffolds for use in tissue engineering. The hydrogel scaffolds are composed of synthetic terpolymers complexed with polyvinyl alcohol (PVA), which facilitate cell-sheet and tissue growth. In the presence of a monosaccharide, the PVA-hydrogel is dissolved and cell-sheets are released for harvesting. Further disclosed herein are methods for producing PVA hydrogels which support tissue growth. Tissue engineering applications and methods are also disclosed. | 09-12-2013 |
20130260464 | CROSSLINKED HYALURONAN HYDROGELS FOR 3D CELL CULTURE - The present invention relates to a new method for treating or processing a cross-linked hyaluronan hydrogel prior to its use in applications involving three-dimensional cell culture. The invention also relates to cross-linked hyaluronan hydrogels prepared by such a method and to their use in 3D cell culture, stem cell/tissue engineering, drug discovery, toxicology testing, and broad cell biology applications. | 10-03-2013 |
20130280807 | CELL CULTURE CHAMBER, METHOD FOR PRODUCING SAME, TISSUE MODEL USING CELL CULTURE CHAMBER, AND METHOD FOR PRODUCING SAME - A single cell-culture chamber is provided that includes a dried vitrigel membrane covering and secured to one open end surface of a tubular frame. Also provided is a double cell-culture chamber that includes two tubular frames of substantially the same planar cross-sectional shape adhesively secured to each other with a dried vitrigel membrane interposed between the opposing open end surfaces of the tubular frames so as to form a first chamber and a second chamber via the dried vitrigel membrane. | 10-24-2013 |
20130288375 | Tissue-Specific Extracellular Matrix With or Without Tissue Protein Components for Cell Culture - The present invention relates to compositions and methods for mimicking an in vivo environment for culturing cells in vitro. The in vivo mimicking environment is based on the generation of a tissue-specific extracellular matrix wherein the matrix provides a substrate for which the cultured cell originated from. The tissue-specific extracellular matrix can further comprise a component of a whole tissue-specific homogenate. | 10-31-2013 |
20130309771 | BIOREACTOR SYSTEM - Disclosed herein are bioreactor systems and methods of utilizing said systems. | 11-21-2013 |
20140038289 | NONADHESIVE SPHERE CELL CULTURE CONTAINER, SYSTEM, AND METHOD - The present invention provides a nonadhesive sphere cell culture container, system and method, which is performed by coating a gel film on the bottom of the culture container, and then by immersing the liquid medium containing cells on the gel film to cultivate cells as the sphere cells. Abundant cancer stem cells with sphere formation can be harvested by the invention with advantages of marked reduction of cost, time-saving (takes only 7-10 days for spheres formation), and without additional separation technique. | 02-06-2014 |
20140038290 | EXTRACELLULAR MATRICES WHICH CAN BE USED AS SCAFFOLD FOR LIVING CELLS - The present invention discloses an extracellular matrix comprising a modified polysaccharide consisting of repeating disaccharide units whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized into a carboxylic acid. | 02-06-2014 |
20140051168 | THREE DIMENSIONAL CELL CULTURE COMPOSITIONS AND METHODS OF USE - Described herein is a three-dimensional cell culture scaffold composition comprising an absorbent rigid (AR) component, and in some embodiments, further comprises a gel component. The absorbent rigid component preferably comprises a glass fiber material. It is a surprising finding of the present invention that an AR component having a void volume of between approximately 70% and 95% results in a three-dimensional cell culture composition that allows for robust, high-throughput screening and high-content screening accessible tissue models with preserved cell morphology, heterogeneity of cell types and cell populations, extracellular matrix constituents, functional cell-cell and cell-extracellular matrix interactions and signaling with sufficient specificities to tissue physiology and pathology. | 02-20-2014 |
20140220687 | METHODS AND SYSTEMS FOR FORMING BIOCOMPATIBLE MATERIALS - Methods and systems forming biocompatible materials are disclosed herein. Forming a biocompatible material may include contacting a liquid, having a linking material, with an adjoining material having embedded therein a nucleating material that causes the linking material to nucleate and grow into the liquid. After a time sufficient to cause the linking material to grow substantially from the nucleating material into a space occupied by the liquid, the liquid may be solidified to form a solid such that the linking material secures the solid to the adjoining material. | 08-07-2014 |
20140220688 | Thermally Induced Gelation Of Collagen Hydrogel And Method Of Thermally Inducing Gelling A Collagen Hydrogel - The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels. | 08-07-2014 |
20140242696 | Thermally Induced Gelation Of Collagen Hydrogel And Method Of Thermally Inducing Gelling A Collagen Hydrogel - The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels. | 08-28-2014 |
20140242697 | Thermally Induced Gelation Of Collagen Hydrogel And Method Of Thermally Inducing Gelling A Collagen Hydrogel - The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels. | 08-28-2014 |
20140315309 | METHOD FOR MANUFACTURING IN VITRO VASCULARIZED TISSUE - The present invention relates to a method for manufacturing an in vitro vascularized tissue, which enables obtaining the in vitro vascularized tissue by inducing in vitro angiogenesis in a tissue using vascular cells, for use in in vitro research of diseases in vascularized tissues and developing a treatment. The method for manufacturing the in vitro vascularized tissue comprises the steps of: supplying to a container for manufacturing tissue a hydrogel in which vascularized tissue cells are mixed; submerging a collection tip to which vascular cells are coupled into the hydrogel; hardening the hydrogel while the collection tip is submerged; and supplying a cell culture fluid to the upper side of the hydrogel. | 10-23-2014 |
20140356954 | METHOD FOR PRODUCING DIFFERENT POPULATIONS OF MOLECULES OR FINE PARTICLES WITH ARBITRARY DISTRIBUTION FORMS AND DISTRIBUTION DENSITIES SIMULTANEOUSLY AND IN QUANTITY, AND MASKING MEMBER THEREFOR - A masking member contains parallel through-holes, each of the through-holes contains a tilted wall structure; an upper end of the tilted wall structure of one of the through-holes abuts on an upper end of the tilted wall structure of an adjacent one of the through-holes thereby forming a knife-edge ridge at the upper ends. The masking member may in contact with a substrate. Formation in quantity of various different populations of a substance being studied with multiple combinations of distribution form and distribution density may be conducted by dripping a suspension of a single concentration of the substance onto the masking member. | 12-04-2014 |
20140377867 | BIOREACTOR FOR CELL GROWTH AND ASSOCIATED METHODS - Apparatuses, systems, and methods are provided for growing and maintaining cells. A three-dimensional matrix, such as a hydrogel material, is seeded with cells and placed in a bioreactor having two compartments. The matrix is supported between the two compartments by first and second porous materials, which engage opposing surfaces of the matrix. A first media stream having certain properties is propagated through the first compartment, where it contacts one surface of the matrix via the first porous material. A second media stream having different properties is propagated through the second compartment such that it contacts the opposite surface of the matrix via the second porous material. Through migration of each stream at least partially into the matrix, various controlled gradients may be established within the matrix, encouraging growth of the cells. Such gradients include osmotic pressure, oscillating osmotic pressure, hydrostatic pressure, oxygen tension, and/or nutrient gradients. | 12-25-2014 |
20160060592 | TISSUE ENGINEERED MODEL - A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame. | 03-03-2016 |
20160102288 | EFFICIENT STEM CELL DELIVERY INTO BIOMATERIALS USING CAPILLARY DRIVEN ENCAPSULATION - Efficient stem cell delivery into biomaterials using capillary driven encapsulation are disclosed herein where stem/progenitor and/or tissue specific cells are rapidly and efficiently seeded via capillary driven encapsulation into a porous scaffold for cell deliver in the skin or any other organ. The rapid capillary force approach maximizes both seeding time and efficiency by combining hydrophobic, entropic and capillary forces to promote active, ‘bottom-up’ cell engraftment. This methodology uses micro domain patterned biopolymers in a porous dry gel to generate capillary pressure to move a viscous stem cell mix from a hydrophobic reservoir into the polymer matrix to promote active cell seeding within the entire gel volume. | 04-14-2016 |