| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435383000 | Method of culturing cells in suspension | 57 |
| 20090117654 | MEDIUM FOR CULTURING HEMATOPOIETIC CELLS AND A METHOD OF CULTURING HEMATOPOIETIC CELLS - Provided are a medium for culturing hematopoietic cells including lysyl oxidase inhibitor and a method of culturing hematopoietic cells using the medium. | 05-07-2009 |
| 20100105138 | PREMOUNTED FLUID CONVEYANCE ASSEMBLY FOR CELL EXPANSION SYSTEM AND METHOD OF USE ASSOCIATED THEREWITH - A premounted fluid conveyance assembly for use with a cell expansion machine comprises a tubing-organizer and a fluid conveyance system. The fluid conveyance system is at least partially attached to the tubing-organizer, and the fluid conveyance system comprises an oxygenator or gas transfer module, a length of tubing and a bioreactor all fluidly interconnected. The premounted fluid conveyance assembly is adapted to be detachably-attached to the cell expansion machine. Accordingly, after a premounted fluid conveyance assembly is used, it is removed from the cell expansion machine by disengaging it from the cell expansion machine. Thereafter, another premounted fluid conveyance assembly can be attached in place of the previously used premounted fluid conveyance assembly. | 04-29-2010 |
| 20090042293 | Cell Culture Apparatus, Cell Culture Method, Cell Culture Program and Cell Culture System - The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The cell culture apparatus includes a culture bag for causing the cells to proliferate, a cell inoculation cassette (or culture bag as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation, a culture medium cassette for storing a culture medium supplied to the culture bag and the cell inoculation cassette, a CCD camera | 02-12-2009 |
| 20090269849 | Bioreactor Apparatus - A bioreactor includes a fluid containment vessel and a rotating mixing element including radial inflow elements configured to direction fluid radially inwardly of the mixing element and axial out flow elements configured to redirect the radial inward flow in an axial direction. Both the vessel and the mixing element are made from plastic and the vessel comprises a flexible bag supported by a rigid housing. In one embodiment, the mixing element is pneumatically driven by the buoyancy of gas introduced into the vessel and trapped within gas entrapment cups formed on the periphery of the mixing element. As the volumetric capacity of the bioreactor increases, the ratio of the diameter of the mixing element to the width of the vessel decreases, and a gap defined between the bottom of the mixing element and the bottom of the vessel increase. | 10-29-2009 |
| 20090269848 | TECHNIQUE FOR CULTURE OF MESENCHYMAL STEM CELL UTILIZING LAMININ-5 - Disclosed is an agent for improving at least one activity selected from the group consisting of the growth activity, adhesion activity and extension activity of mesenchymal stem cells, which comprises laminin-5 as an active ingredient. A method of culturing mesenchymal stem cells; a method of isolating mesenchymal stem cells; and a medium, vessel or sheet for use in culturing mesenchymal stem cells are also provided. | 10-29-2009 |
| 20090061516 | ANIMAL CELL CULTURE MEDIA COMPRISING NON ANIMAL OR PLANT DERIVED NUTRIENTS - The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of tile present invention can be used for growth of animal cells for virus production. | 03-05-2009 |
| 20110287541 | ACCORDION BIOREACTOR - Disclosed herein are bioreactors including a first sheet and a second sheet (one or both of which is substantially transparent to light), wherein the second sheet is disposed adjacent to the first sheet, and the first and second sheets are sealed along a first longitudinal edge, a second longitudinal edge, a first horizontal edge, a second horizontal edge, and at least one intermediate horizontal seal between the first horizontal edge and the second horizontal edge, thereby forming at least two chambers for holding fluid in series along a vertical axis, wherein each of the two or more chambers is oriented at an angle relative to the vertical axis, wherein the angle is about 0° to about 90° and at least one of the chambers is oriented at an angle greater than 0°, and wherein there is at least one opening in each of the first horizontal edge, the second horizontal edge, and intermediate horizontal seal(s); a support structure comprising at least one horizontal support, wherein the horizontal support is located at or near the position of the intermediate horizontal seal; a reservoir below the second horizontal edge of the first and second sheets; and a means for pumping fluid from the reservoir to the first horizontal edge of the first and second sheets. Also disclosed are methods of culturing cells including circulating a suspension of cells in a disclosed bioreactor. In some examples, the cells include microalgae, macroalgae, bacteria, fungi, insect cells, plant cells, animal cells (such as mammalian cells), or plant or animals tissue or organs. In particular examples, the method includes exposing the culture in the bioreactor to a light source (such as sunlight or an artificial light source). | 11-24-2011 |
| 20090298180 | CELL CULTURE APPARATUS AND METHOD - A cell culture apparatus includes a housing, a bag and an inflatable bladder. The bag is disposed in the housing and has an interior surface defining a chamber for culturing cells. The bladder is disposed in the housing, external to the bag, and is sufficiently inflatable and expandable to exert pressure on the bag when the chamber is substantially free of fluid. The bladder may be inflated and expanded to reduce movement of the bag within the housing. Alternatively, or in addition, the bladder may be inflated and expanded to apply a mechanical stress on cells cultured within the chamber of the bag. | 12-03-2009 |
| 20100112698 | Device for Trapping Extraneous Gas - A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside. | 05-06-2010 |
| 20100041146 | THREE-DIMENSIONAL CELL CULTURE CARRIER AND METHOD FOR CELL CULTURE USING THE SAME - A three-dimensional cell culture carrier of the present invention includes a fibrous structure having a three-dimensional space for cell culture. The fibrous structure is formed of a plurality of intertangled fibers. Each of the fibers used in the fibrous structure is made of a material having a visible light transmittance of 40% or more (preferably 50% or more) when it is formed into a shape having a thickness of 3 mm. Examples of such fibers include glass fibers. The fiber has an aspect ratio of 1 or more, and a fiber diameter of 100 μm to 700 μm. | 02-18-2010 |
| 20100099189 | Methods for Rejuvenating Cells In Vitro and In Vivo - The present invention provides methods for rejuvenating cells, tissues and the whole body. Also provided are rejuvenating buffers and agents as well as kits for rejuvenating cells. Also provided are methods for dedifferentiating somatic cells and differentiating the cells into other cell types. | 04-22-2010 |
| 20080241925 | Three-Dimensional Self Assembly in Suspension of Adherent Cells - Methods and vessels for three-dimensional self-assembly of cells in suspension. The methods and vessels involve pre-coating a cell culture vessel with an adhesion-inhibiting substance such as Pluronic prior to culturing a cell therein. | 10-02-2008 |
| 20120295352 | Systems and Methods for Expanding High Density Non-Adherent Cells - Embodiments described herein generally relate to systems and methods for promoting the expansion of high density non-adherent cells through the use of a cell growth chamber, a mass transfer device, and a fluid circulation loop. Improved cell growth is achieved in the cell growth chamber by using a chamber having a particular orientation and shape, e.g., conical, to create a media-rich reservoir for growing cells. By placing the chamber in a vertical position, the force of media flow along the chamber walls is substantially equal and opposite to the gravitational force on the cells. The interaction of these forces maintains the non-adherent cells in suspension. The use of the cell growth chamber in conjunction with the mass transfer device and fluid circulation loop(s) creates efficiencies by relying on the cumulative and combined features of the devices. | 11-22-2012 |
| 20100144037 | Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves. | 06-10-2010 |
| 20100184219 | Methods and Apparatuses Relating to Cell Culture Media - A method of preparing cell culture media for use in culturing cells and an apparatus for carrying out the method. The method includes providing a vessel containing cell culture media and exposing the cell culture media to a first controlled atmosphere having a predetermined O | 07-22-2010 |
| 20080213894 | Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves. | 09-04-2008 |
| 20110008893 | MEDIUM FOR MAMMALIAN SOMATIC CELLS AND ADDITIVE THEREFOR - Disclosed are a medium for mammalian somatic cells with which mammalian somatic cells can be grown effectively when the mammalian somatic cells are cultured, while reducing the amount of serum to be added to the medium as much as possible or without adding serum thereto, and an additive to constitute the medium. By blending of a ligand for an endothelial cell differentiation gene (Edg) family receptor and a ligand for a serotonin receptor to a medium, somatic cells of mammals can be grown even in cases where the medium does not contain serum at all or contains only a small amount thereof. | 01-13-2011 |
| 20110076770 | CULTURING METHOD OF MESENCHYMAL STEM CELL - To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 g/ml at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring. | 03-31-2011 |
| 20100062530 | CELL CULTURE METHOD, CELL CULTURE SYSTEM AND CULTURE MEDIUM ADJUSTEMENT APPARATUS - In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps of | 03-11-2010 |
| 20100055790 | CELL CULTURE APPARATUS AND ASSOCIATED METHODS - A cell culture apparatus consists of a vessel or array of vessels, each comprising a substantially flat bottom and substantially vertical sides, and composed of a substantially gas permeable material. The inner bottom surface of the vessel is commonly textured to provide the cells with adequate features for attachment and spreading. The vessel may include an integral annular flange which can be used to suspend the vessel from a suspensory element of a rack structure. To promote cell attachment and growth, the inner surfaces of the vessel bottom and sides may be coated with commonly available bio-active materials. Cells cultured in the system commonly grow in three dimensions for extended periods of time, and often produce significantly higher quantities of cellular products than cells grown in conventional labware. | 03-04-2010 |
| 20090215176 | Differential Pressure Pump System - The present disclosure is directed to a fluid pumping mechanism that uses differential pressure to drive flow through one or more culture chambers. Fluid contained within a first chamber can be caused to flow through one or more culture chambers and thence to a second chamber upon establishment of a pressure differential between the two chambers. The differential pressure system can induce either steady state or pulsatile flow through a culture chamber. In one embodiment, a culture chamber can be held at a high or low pressure hydrostatic state through utilization of the disclosed systems. | 08-27-2009 |
| 20100279411 | COMBINATIONS OF PROTEINS TO ENHANCE VIABILITY OF STEM CELLS AND THEIR PROGENITORS BEFORE TRANSPLANTATION - Embodiments of the present invention include the use of placental alkaline phosphatase alone or in combination with human transferrin and, optionally, human α | 11-04-2010 |
| 20100035342 | METHOD FOR CONTROLLING pH, OSMOLALITY AND DISSOLVED CARBON DIOXIDE LEVELS IN A MAMMALIAN CELL CULTURE PROCESS TO ENHANCE CELL VIABILITY AND BIOLOGIC PRODUCT YIELD - Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO | 02-11-2010 |
| 20100323447 | MICROFLUIDIC CELL CULTURE DEVICE AND METHOD FOR USING SAME - Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid. | 12-23-2010 |
| 20100190253 | CELL CULTURE CONTAINER AND CELL CULTURE METHOD - To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber ( | 07-29-2010 |
| 20100029000 | Assembly and methods for cell or tissue culture and treatment - The invention provides an assembly designed under the multiwell plate make and methods of using the same for dynamic-based cell culture and pharmacokinetic-based cell treatment. The assembly comprises a multiwell plate and a lid which permits continuously adding and removing culture medium for culturing cells or tissues in vitro. The dynamic medium makes it possible to culture cells or tissues exposing to changing concentration of a drug candidate over time. | 02-04-2010 |
| 20110003390 | METHOD AND DEVICE FOR CELL CULTURE IN OPEN CONTINUOUS MODE - The present invention relates to the use of cell cultures in the open continuous mode, to a method for selecting static cell variants or cell variants which proliferate in suspension, to a culture substrate and to a device suitable for implementing this method. | 01-06-2011 |
| 20120045834 | METHOD AND APPARATUS FOR MAINTAINING MICROCARRIER BEADS IN SUSPENSION - The invention relates to an impeller for growing adherent mammalian cells on microcarrier beads. In other aspects, the invention relates to methods of using the impeller and to bioreactors or cell separators comprising the impeller. | 02-23-2012 |
| 20120045833 | Bioreactor, control system and control method thereof - A bioreactor includes a cylinder, a mandrel and a filter film. The cylinder includes two end walls and a circumferential wall interconnected with the both end walls; the two end walls and circumferential wall define together a reaction chamber. The mandrel is disposed between the two end walls. The mandrel has an inlet path and an outlet path provided on its both ends; the filter film wraps the mandrel to prevent passage of the first kind of substance while permit the passage of the second kind of substance; a gap is defined between the filter film and mandrel; at least one portion of the filter film is tied so as to divide the gap into multiple separate and isolated gap regions to prevent the second fluid from direct exiting from the outlet path through the gap. Also disclosed are control system and method for the bioreactor. | 02-23-2012 |
| 20090035856 | CONTINUOUS PERFUSION BIOREACTOR SYSTEM - Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein. | 02-05-2009 |
| 20080293138 | BIOLOGICALLY ACTIVE SUBSTANCE TRANSFER SHEET, CELL CULTURE KIT CONSTITUTED OF CELL CULTURE PLATE AND BIOLOGICALLY ACTIVE SUBSTANCE TRANSFER SHEET, PRODUCING METHOD THEREOF AND METHOD FOR SCREENING CELL CULTURE CONDITIONS UTILIZING THE SAME - The invention provides a biologically active substance transfer sheet which can be formed with simple steps, a cell culture kit which comprises a cell culture plate and a biologically active substance transfer sheet, a producing method therefor, and a method for screening cell culture conditions with cells. The transfer sheet is prepared by providing biologically active substances having biological activity in plural areas on a sheet base, and biologically active substances are supplied from the sheet to culture regions provided on the plate. | 11-27-2008 |
| 20120058559 | Monocyte Activation Test Better Able to Detect Non-Endotoxin Pyrogenic Contaminants in Medical Products - An improved monocyte activation test is described that is better able to detect non-endotoxin pyrogens in medical products, in which a sample is incubated with a monocyte-containing reagent in an assay system comprising at least one surface comprising polypropylene. The invention also concerns assay systems for use in these tests that include at least one microtiter well having at least one interior surface comprising polypropylene and having a shape such that monocyte-containing reagent is concentrated in the well to provide greater cell to cell contact. The invention also relates to a diagnostic kit that can be used to test for the presence of non-endotoxin pyrogens in a sample. | 03-08-2012 |
| 20080299656 | ISOLATION OF MULTI-LINEAGE STEM CELLS - The present application discloses a method of manipulating a biological sample of cells, which includes multi-lineage stem cells, progenitor cells, other marrow stromal cells: allowing the sample of cells to settle in a container; transferring supernatant from the container to another container; and isolating cells from the supernatant, which has comparatively lower density in the sample. | 12-04-2008 |
| 20110003389 | CELL CULTURE INSTRUMENT AND CELL CULTURE METHOD USING THE SAME - Provided is a cell culture instrument which has excellent operability while including a plurality of wells capable of holding cells. The cell culture instrument according to the present invention includes a base portion in which a well group including a plurality of first wells capable of holding cells is formed, and a frame portion vertically arranged around the well group of the base portion to form a second well being capable of holding a solution. | 01-06-2011 |
| 20100129910 | Cell matrix related compositions and their use for generating embryoid bodies - The present invention relates to compositions and methods for the generation of embryoid bodies from embryonic stem cells, and for aggregation of ES cells for establishing ES cell colonies from small numbers of cells. Compositions of the invention, in certain embodiments, comprise embryonic stem cells, an extracellular matrix molecule, and a molecule capable of cross linking. The present invention also relates to methods for embryoid body formation using a composition of the invention and to cells and tissues isolated or derived from an embryoid body generated with a composition or method of the invention. | 05-27-2010 |
| 435384000 | Culture medium contains a growth factor or growth regulator | 8 |
| 20090305413 | MULTIPOTENT ADULT STEM CELLS HAVING AN ABILITY OF OCT4 EXPRESSION DERIVED FROM UMBILICAL CORD BLOOD AND METHOD FOR PREPARING THE SAME - The present invention relates to multipotent adult stem cells expressing Oct4, derived from umbilical cord blood (UCB) and also these cell are expressing CD29, CD31, CD44, simultaneously, a method for preparing the same, and more specifically to multipotent adult stem cells which are obtained by culturing umbilical cord blood-derived monocytes in a medium containing bFGF (basic fibroblast growth factor) and human serum or plasma. In addition, multipotent adult stem cells expressing Oct-4 from UCB are morphologically spindle or round shaped cells Although the stem cells according to the present invention are adult stem cells, they are multipotent and capable of differentiating into ectodermal-, messodermal-, endodermal-originated tissue or cells including osteogenic cells or nerve cells etc, thus they can be effectively used in the treatment of intractable diseases and incurable diseases. | 12-10-2009 |
| 20090170201 | COMPOSITIONS AND METHODS OF MAKING EMBRYONIC STEM CELLS - The invention relates to cell proliferation, cell differentiation, male infertility, male fertility and to compositions and methods involved therein. | 07-02-2009 |
| 20090170200 | STEM CELL MEDIUM - A medium for culturing stem cell. The stem cell medium of the invention comprises a fetal bovine serum, one or plurality of amino acid, one or plurality of vitamin, one or plurality of growth factor, one or plurality of inorganic salt, one or plurality of antioxidant, wherein the stem cell medium has a calcium concentration of less than about 1.8 mM, and the fetal bovine serum is present in an amount of less than about 10% by volume of the medium. The stem cell medium of the invention can maintain the proliferative and self-renewal capacity of the stem cells and keep stem cells at a steady stage. | 07-02-2009 |
| 20090053811 | Reactor Comprising an Annular Reaction Space - A biological or chemical reactor ( | 02-26-2009 |
| 20100279412 | CULTURE MEDIUM ADDITIVE FOR USE IN SERUM-FREE CULTURING OF ANIMAL CELL, KIT AND USE THEREOF - Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium. | 11-04-2010 |
| 20120214237 | COMPOSITIONS AND METHODS USEFUL FOR CULTURING DIFFERENTIABLE CELLS - The present invention relates to cell culture methods and compositions that are essentially serum-free and comprise a basal salt nutrient solution and an ErbB3 ligand. | 08-23-2012 |
| 435385000 | Medium contains a colony stimulating factor | 1 |
| 20100041147 | HEMOGLOBIN ALPHA CHAIN PEPTIDE FRAGMENTS USEFUL FOR INHIBITING STEM CELL PROLIFERATION - Disclosed and claimed are methods for the isolation and use of stem cell inhibiting factors for regulating the abnormal stem cell cycle and for accelerating the post-chemotherapy peripheral blood cell recovery. Also disclosed and claimed are the inhibitors of stem cell proliferation. | 02-18-2010 |
| 435387000 | Medium contains a polypeptide hormone | 1 |
| 20090233360 | Methods and Compositions for Culturing of neural Precursor Cells - The present invention provides methods and compositions for the propagation and expansion of neural precursor cells (NPCs). NPCs may be used in the clinical implementation of stem cell therapy to treat disorders such as Parkinson's disease, Huntington's disease, neuropathic pain and other diseases of the central nervous system. The large-scale production of NPCs in bioreactors allows for the generation of clinical quantities of these cells. | 09-17-2009 |
| 435388000 | Culture medium contains an albumin | 1 |
| 20080268538 | Device and Method to Prevent Culture Media Degradation - The present invention provides a device for cell culture. The device comprises a culture zone, a selective sorption zone and culture media transfer means. Culture media is transferred or recirculated from the culture zone through the selective sorption zone which comprises at least one agent reactive with cytotoxic degradation products present in the culture media and back to the culture zone via the culture media transfer means. | 10-30-2008 |
| 435391000 | Culture medium contains an animal extract | 1 |
| 435392000 | Serum | 1 |
| 20130130382 | AUTOSERUM-CONTAINING BONE MARROW CELL CULTURE SYSTEM, AUTOSERUM-CONTAINING BONE MARROW CELL CULTURE METHOD, AND METHOD FOR PRODUCING MEDICINAL COMPOSITION COMPRISING AUTOSERUM-CONTAINING CULTURED BONE MARROW CELLS AS ACTIVE INGREDIENT - To provide an autoserum-containing bone marrow cell culture system, whereby bone marrow cells, which are collected from a subject without using an anticoagulant, are subjected to an anticoagulation treatment using a medium in a liquid-tight state, cultured and then further cultured using the serum of said subject which is prepared in a liquid-tight state; an autoserum-containing bone marrow cell culture method; and a method for producing a medicinal composition which comprises, as the active ingredient, autoserum-containing cultured bone marrow cells. [Solution] An autoserum-containing bone marrow cell culture system for culturing bone marrow cells, which are collected from a subject without using an anticoagulant, using the serum of said subject, said system comprising a bone marrow cell suspension-storing device, a collected blood-storing device, an autoserum-acquiring device, and a bone marrow cell-culturing device. | 05-23-2013 |
| 435393000 | Using airlift or laminar flow aeration or foam culture | 2 |
| 20090286318 | Modules for Membrane Aeration - The present invention relates to a device for the aeration of liquid media by means of tube aeration, special tube modules contained therein and the use of a device for the aeration of liquid media. | 11-19-2009 |
| 20110097800 | METHOD AND APPARATUS FOR RETAINING AND RECIRCULATING CELLS - The invention relates to a device and a method for retaining and recirculating cells in a vessel through which flow passes continuously or batchwise. | 04-28-2011 |
| 435394000 | Wherein culture vessel is rotated or oscillated or culture is agitated | 10 |
| 20100035343 | SYSTEM AND METHOD FOR CONTROLLING A MAMMALIAN CELL CULTURE PROCESS - A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow. | 02-11-2010 |
| 20100124782 | Composition For Repairing Defect In Skin Or Gingival Soft Tissue And Method Of Culturing Autologous Fibroblasts - The object of the present invention is to provide a method for producing aggregates of autologous fibroblasts for producing autologous fibroblasts having been aggregating in a fibrous state, and an aggregate produced by this method. Another object is to provide a composition for repairing a defect in a skin or gingival soft tissue which can keep favorable treatment effect by remaining in the treated area over a long period of time, and an injection having this composition. | 05-20-2010 |
| 20110207218 | MIXING SYSTEM WITH CONDENSER - A system for mixing a liquid solution or suspension includes a support housing bounding a compartment and a collapsible bag disposed within the compartment. A mixer is disposed within the collapsible bag while a sparger delivers a gas to the lower end of the collapsible bag. A gas outlet line extends from an upper end of the collapsible bag to a condenser assembly. A gas exhaust line and a fluid collection line both extend from the condenser assembly. | 08-25-2011 |
| 20090111179 | Cell Culture Shaking Device and Shaking Culture Method as Cell Culture Method - The invention intends to provide a cell culture shaking device which is able to improve the efficiency of cell culture while preventing the cells from becoming damaged. The shaking devices used in cell culture apparatuses for culturing cells in flexible culture bags for storing culture suspension having cells inoculated therein includes: shaking mechanisms having operating panels for pressing the culture bags repeatedly, so that the culture suspension in the culture bags is stirred by being pressed by a plurality of projections projecting for the operating panels. | 04-30-2009 |
| 20080213895 | Method for culturing dendritic cells (DC) and cytokine-induced killer cells (D-CIK) and applications thereof - A mass production of dendritic cells (DC) and cytokine-induced killer cells (D-CIK) cells from the umbilical cord blood or peripheral blood via the activation with a cellular activator “Koyo” derived from the purified tumor cells during the aggregation and proliferation of the purified tumor cells is provided. A preparation of a vaccine with the dendritic cells (DC) and D-CIK cells is used to improve the symptoms of infectious diseases, cancers, metastasised cancers and auto-immune disorders. | 09-04-2008 |
| 20110027887 | METHOD OF DYNAMICALLY CULTURING EMBRYONIC STEM CELLS - The present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy. | 02-03-2011 |
| 20080220523 | CELL EXPANSION SYSTEM AND METHODS OF USE - Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided. | 09-11-2008 |
| 20110165677 | METHOD FOR REDUCING DEPOSITS DURING THE CULTIVATION OF ORGANISMS - The invention relates to a process for reducing the amount of deposits during the culture of organisms, and in particular of cell cultures, where these have a tendency towards agglomeration or adhesion to the bioreactor and to its elements, or where these are readily susceptible to agglomeration or adhesion of cell debris or of substances. | 07-07-2011 |
| 20090130757 | BIOREACTOR WITH MIXER AND SPARGER - A bioreactor and related methods are for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element driven by an external motive device. In one embodiment, the bioreactor includes a mixer and a movable sparger. The mixer may take the form of a rotational wand mixer, and may further comprise a magnetic impeller. In another embodiment, the bioreactor includes a rotational wand mixer and an integral sparger. In still another embodiment, the bioreactor comprises a bag including a rotational wand mixer having rigid blades attached thereto. | 05-21-2009 |
| 20100210016 | ROTATION SYSTEM FOR CELL GROWTH CHAMBER OF A CELL EXPANSION SYSTEM AND METHOD OF USE THEREFOR - A system and method for rotating a cell growth chamber of a cell expansion system includes a rotatable member for engaging a chamber coupling attached to the cell growth chamber. The rotatable member includes an independently operable mechanism for engaging a rotatable fitting associated with the chamber coupling. In at least one embodiment, the chamber coupling is selectively rotatable by turning the rotatable member, thereby rotating the cell growth chamber around a first axis. The cell growth chamber is also selectively rotatable around a second axis by turning the rotatable fitting associated with the chamber coupling. Other novel aspects include a way of attaching the cell growth chamber to the shaft assembly, and a new tube routing clip. | 08-19-2010 |
