Entries |
Document | Title | Date |
20080227197 | ALLOGENEIC STEM CELL BANK - This invention provides methods for supplying a therapy for individuals exposed to radiation following a nuclear event, through the prospective establishment of an undesignated allogeneic stem cell bank with prospective HLA typing of healthy potential recipients. | 09-18-2008 |
20080268535 | Method for Inducing the Differentiation of Human Myelogenous Leukemia Cells Into Megakarocytes or Thrombocytes - Disclosed herein is a method of inducing the differentiation of leukemia cells derived from human bone marrow into megakaryocytes or thrombocytes, comprising the steps of: (a) culturing OP9 cells; (b) layering leukemia cells derived from human bone marrow over the OP9 cells; and (c) incubating the cells in the presence of a compound ((R)-NALPCE) represented by Chemical Formula 1. | 10-30-2008 |
20090081783 | Method for producing langerhans cells or interstitial dendritic cells or both from CD14+ monocytes - The present invention relates to a method for preparing Langerhans cells or interstitial dendritic cells, or both, from CD14 | 03-26-2009 |
20090081784 | GENERATION OF CLONAL MESENCHYMAL PROGENITORS AND MESENCHYMAL STEM CELL LINES UNDER SERUM-FREE CONDITIONS - Methods for obtaining multipotent mesenchymal stem cells under serum-free conditions and methods for identifying multipotent mesenchymal progenitor cells are disclosed. | 03-26-2009 |
20090093056 | Adult Stem Cell-Derived Connective Tissue Progenitors for Tissue Engineering - Methods of generating and expanding proliferative, multipotent connective tissue progenitor cells from adult stem cells are provided. Also provided are methods of generating functional tendon grafts in vitro and bone, cartilage and connective tissues in vivo using the isolated cell preparation of connective tissue progenitor cells. | 04-09-2009 |
20090191628 | Methods for co-culturing cord blood derived cells with menstrual stem cells - Methods are provided for obtaining expanded human cord blood cells expressing CD34. The methods involve seeding a sufficient amount of cord blood cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord blood cells, and co-culturing the cord blood cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord blood cells. Methods are also provided for growing expanded human cord blood cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided. | 07-30-2009 |
20090246869 | Differentiation of Primate Pluripotent Stem Cells to Hematopoietic Lineage Cells - The invention provides methods of differentiating primate pluripotent stem cells into cells of hematopoietic lineage. The invention further provides hematopoietic lineage cells differentiated from primate pluripotent stem cells, as well as methods of using the same and kits comprising the same. | 10-01-2009 |
20090305406 | Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method - The invention relates to a novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for approximately one to three weeks in CellGro™ Hematopoietic Stem Cell Medium, certified for the clinical use, with an addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of the cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under the standard conditions for the cultivation of tissue cultures. | 12-10-2009 |
20090305407 | Devices and Methods for Sampling Biological Fluids - Devices, instruments, systems and methods are provided in which a primary line that receives a biological fluid is selectively sampled by a plurality of collection chambers. Selective sampling occurs by selectively accessing the primary line and selective engagement of a sampling pump under control of a microprocessor. Further, the instrument housing reversibly houses a drive assembly and sample collection housing to permit the interchangeability of drive assemblies and collection housings and thus enhance sterility or reduction of cost. | 12-10-2009 |
20100047908 | MONOCYTE-DERIVED STEM CELLS - Methods for generating multipotent stem cells from adult peripheral blood monocytes are provided. Monocytes may be de-differentiated into monocyte-derived stem cells (MDSCs) by contacting the monocyte with the de-differentation factors, leukocyte inhibitory factor, macrophage colony-stimulating factor, or a combination thereof. The MDSCs may be differentiated into many different types of cells upon contact with the appropriate differentiation factors. Also provided are compositions comprising the MDSCs or differentiated cells derived from the MDSCs. | 02-25-2010 |
20100081196 | Method for Separating a Volume of Whole Blood Into At Least Three Components - A method for separating a volume of whole blood contained in a separation bag into at least three components comprising centrifuging the separation bag so as to separate therein a first inner layer comprising plasma, a second intermediate layer comprising platelets, a third intermediate layer comprising lymphocytes, monocytes and granulocytes and a fourth outer layer comprising red blood cells, transferring, into a plasma component bag connected to the separation bag, a plasma component and transferring into a mononuclear cell component bag a mononuclear cell components. | 04-01-2010 |
20100105135 | POROUS MEMBRANE DEVICE THAT PROMOTES THE DIFFERENTIATION OF MONOCYTES INTO DENDRITIC CELLS - Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other in vitro-generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses. | 04-29-2010 |
20100120144 | METHOD OF CULTURING CELLS - A blood component such as platelets is concentrated. The concentrate, such as platelet-rich plasma, is used in a cell culture medium to grow and proliferate cells. The cells may be from the same person from which the blood concentrate is obtained. The cells grown in the culture medium may be used to treat a patient which may be the same patient from which the blood was extracted and/or the cells were obtained. | 05-13-2010 |
20100144034 | Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of Notch gene - There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which method comprises introducing a Notch gene and/or a Notch signaling related gene into the cells, wherein the finally obtained differentiated cells are the result of cell division of the bone marrow stromal cells into which the Notch gene and/or Notch signaling related gene have been introduced. The invention also provides a method of inducing further differentiation of the differentiation-induced neural cells to dopaminergic neurons or acetylcholinergic neurons. The invention yet further provides a treatment method for neurodegenerative and skeletal muscle degenerative diseases which employs neural precursor cells, neural cells or skeletal muscle cells produced by the method of the invention. | 06-10-2010 |
20100151569 | METHOD OF PRODUCING A POPULATION OF CELLS - A method is provided for producing a population of post-mitotic cells of the neutrophil lineage, which method comprises the ex vivo steps of: (a) providing a population of cells comprising neutrophil progenitor cells; and (b) culturing the population of cells in an animal cell culture medium comprising (i) one or more early acting cytokines and (ii) one or more cytokines that differentiate said progenitor cells into a neutrophil specific lineage, under conditions of low oxidative stress, the culture medium being agitated when the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and the progeny thereof under static conditions, to produce a population of post-mitotic cells of the neutrophil lineage. The resulting population of cells can be used to increase the number of neutrophils in a patient. | 06-17-2010 |
20100197016 | Structure enclosing hematopoietic progenitor cells from ES cells and method for preparing blood cells using the same - An object of the present invention is to provide a sac-like structure enclosing hematopoietic progenitor cells and a method for preparing the sac-like structure as well as a method for efficiently preparing blood cells such as mature megakaryocytes and platelets from the sac-like structure. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, the sac-like structure being obtained by plating ES cells onto feeder cells and culturing the ES cells under suitable conditions for inducing hematopoietic progenitor cell differentiation. Moreover, the present invention provides a method for producing various blood cells, the method comprising further culturing hematopoietic progenitor cells enclosed in the sac-like structure under suitable conditions for inducing blood cell differentiation. | 08-05-2010 |
20100197017 | METHOD FOR STERILIZATION OF BIOLOGICAL PREPARATIONS - Provided is a method for the sterilization of a biological preparation including desired viable biological entities. The method includes irradiating a dried (e.g. freeze-dried) biological preparation with UV radiation at an intensity and for a duration sufficient to reduce the amount or activity of living-matter contaminants in the biological preparation, the intensity and duration selected such that at least part of the desired biological entities in the sample remains viable. The described method is particularly suitable for the reduction of the amount or activity of contaminants such as bacteria or viruses from biological preparations including red blood cells or platelets. | 08-05-2010 |
20100227401 | MEDIA AND PROCESSES FOR THE EX VIVO PRODUCTION OF MEGAKARYOCYTES FROM HUMAN CD34+ CELLS - Disclosed herein are media and processes for the ex vivo production of megakaryocytes from human CD34 | 09-09-2010 |
20100267137 | METHODS FOR INDUCING THE DIFFERENTIATION OF BLOOD MONOCYTES INTO FUNCTIONAL DENDRITIC CELLS - Methods are provided for treating blood monocytes to produce functional antigen presenting dendritic cells. An extracorporeal quantity of a subject's blood is treated to separate the blood into a plasma component containing proteins, a platelet component and a buffy coat component. A plastic treatment device is provided having plastic channels that allow transmittance of light to the interior of the plastic device and a light source that produces light of a wave length selected to activate the photoactivatable agent. The plasma component containing proteins is first pumped through the plastic treatment device, followed by the platelet component and finally the buffy coat component. The resulting treated cells may be incubated or reinfused directly to the subject. | 10-21-2010 |
20100317106 | HARVESTING TARGET MATERIALS FROM CENTRIFUGED SUSPENSIONS - Embodiments of the present invention are directed to harvesting a target material from a suspension using a tube and float system. A suspension suspected of containing a target material is combined with a solution having one or more labels that distinguish the target material from other materials in the suspension. The tube, float, and suspension are centrifuged to separate various materials in the suspension according to associated specific gravities. The float expands the axial length of the target material layer and displaces the target material to a narrow space between the float and the inner wall of the tube. The space is illuminated with light that causes the labels to emit light identifying the location of the target material within the tube. One or more openings can then be formed in the tube at or near the point where the target material is located and the target material harvested. | 12-16-2010 |
20110014696 | METHOD FOR PRODUCING DENDRITIC CELLS - Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified. | 01-20-2011 |
20110045589 | Methods Of Controlling Proliferation And Differentiation Of Stem And Progenitor Cells - A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate | 02-24-2011 |
20110053268 | METHOD FOR DIAGNOSIS OF ABNORMAL IRON METABOLISM USING ACTIVE HEPCIDIN AS INDICATOR - The present inventor employed surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to analyze distinctive serum proteomic patterns of hemodialysis patients. The present inventor found three peptides at 2,192, 2,789, and 2,851 m/z that showed a significant correlation with the levels of serum ferritin. The molecular sizes of the 2,192 and 2,789 m/z matched well with the reported sizes of hepcidin-20 and -25, respectively, and the latter completely coincided with the size of synthetic hepcidin-25. It would be possible to diagnose abnormal iron metabolism by detecting bioactive forms of hepcidin in the serum. | 03-03-2011 |
20110070644 | Monocyte-Derived Stem Cells (MDSC) and Methods of Use Thereof - Monocyte derived adult stem cells (MDSCs) isolated from peripheral blood of mammals is provided, along with pharmaceutical compositions containing an MDSC, kits containing a pharmaceutical composition, and methods of preparing, propagating and using MDSCs or differentiated derivatives thereof The uses of these biological materials include methods of treating disorders or diseases, as well as methods of ameliorating a symptom associated with any such disorder or disease. | 03-24-2011 |
20110177595 | Multipotent Adult Stem Cells, Sources Thereof, Methods of Obtaining and Maintaining Same, Methods of Differentiation Thereof, Methods of Use Thereof and Cells Derived Thereof - Methods and compositions are provided for circularizing target sequences in a sample. In particular, ligation oligonucleotides are employed to selectively hybridize with the target such that the target can be ligated into a closed circular target. Rolling circle amplification can then be performed directly on the target sequence for subsequent detection and analysis. | 07-21-2011 |
20110201111 | METHOD FOR OBTAINING CONNECTIVE MESENCHYMAL STEM CELLS FROM THE MONONUCLEAR FRACTION OF HUMAN BONE MARROW - The present invention relates to a method for obtaining connective mesenchymal stem cells from the mononuclear fraction of human bone marrow cells using type AB human serum as a supplement. Said method includes the steps of obtaining said mononuclear fraction of bone marrow, the step of recovering and pre-expanding the connective stem cells and the step of expanding the connective stem cells until obtaining the clinical dose required for the therapeutic use thereof. | 08-18-2011 |
20110207216 | Synthetic Peptide (Meth) Acrylate Microcarriers - A process for forming microcarriers includes contacting an initiator-conjugated microcarrier base with one or more monomers and activating the initiator to initiate polymerization and to graft a polymer from the base via the initiator or a remnant thereof. At least one of the monomers is conjugated to a polypeptide so that incorporation of the monomer into the forming polymer conjugates the polypeptide to the polymeric coating as it is formed in situ. | 08-25-2011 |
20120003737 | MULTIPOTENT STEM CELLS AND USES THEREOF - The invention provides a quiescent stem cell having the capacity to differentiate into ectoderm, mesoderm and endoderm, and which does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105 and CD90. The invention further provides a proliferative stem cell, which expresses genes including Oct-4, Nanog, Sox2, GDF3, P16INK4, BMI, Notch, HDAC4, TERT, Rex-1 and TWIST but does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105 and CD90. The cells of the invention can be isolated from adult mammals, have embryonic cell characteristics, and can form embryoid bodies. Methods for obtaining the stem cells, as well as methods of treating diseases and differentiated the stem cells, are also provided. | 01-05-2012 |
20120003738 | Method For Amplification And Functional Enhancment Of Blood Derived Progenitor Cells Using A Closed Culture System - The present invention provides a method for expanding and improving functional capacity of human adult-derived progenitor cells in vitro using a closed culture system. The present invention provides a favorable condition for cell therapy to promote tissue repair and organogenesis via vasculogenesis and angiogenesis in clinical settings. The proposed closed bag culture system for culturing hemangioblast comprises of, in one embodiment, a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3, thrombopoietin, and vascular endothelial growth factor and a kit for the preparation of the serum-free culture medium and the like. | 01-05-2012 |
20120009676 | GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM SMALL VOLUMES OF PERIPHERAL BLOOD - Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells. | 01-12-2012 |
20120040458 | METHOD FOR PRODUCING DENDRITIC CELLS - An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof. | 02-16-2012 |
20120064624 | METHOD FOR INHIBITING THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS BY USING CD13 RECEPTOR - The present invention discloses a method for inhibiting the growth of | 03-15-2012 |
20120122214 | METHOD FOR PRODUCING CELL MEDICINE - It is an object of the present invention to provide a method for producing a cell medicine that is effective for diseases while causing a low risk to these diseases. The present invention provides a method for producing a phagocyte that expresses a foreign protein, which comprises: a step of introducing a protein expression vector into an induced pluripotent stem cell; and a step of inducing the induced pluripotent stem cell, into which the protein expression vector has been introduced, to differentiate into a phagocyte. | 05-17-2012 |
20120220032 | OPTIMIZING CULTURE MEDIUM FOR CD34<+> HEMATOPOIETIC CELL EXPANSION - The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention. | 08-30-2012 |
20120282693 | METHOD OF GENERATING NATURAL KILLER CELLS AND DENDRITIC CELLS FROM HUMAN EMBRYONIC STEM CELL-DERIVED HEMANGIOBLASTS - This invention provides methods of generating natural killer (NK) cells and dendritic cells (DCs). The methods utilize human hemangioblasts as intermediate cells to generate the NK cells and DCs. In various embodiments, the methods do not require the use of stromal feeder layers. | 11-08-2012 |
20120329153 | CAR RECEPTOR AS A MEDIATOR OF MIGRATORY CELL CHEMOTAXIS AND/OR CHEMOKINESIS - This invention relates to methods and compositions for modulating movement of eukaryotic cells with migratory capacity. More specifically, the invention relates to methods and compositions for modulating movement of CaR receptor expressing cells of hematopoietic, neural, epithelial, endothelial, or mesenchymal origin, in a specific site in a subject. The foregoing are useful, inter alia, in the treatment of conditions characterized by a need to modulate migratory-cell movement associated with specific sites in a subject. Specific sites include sites of inflammation and modulation of migratory-cell movement is movement away from an agent source, or repulsion. The invention also relates to methods for manipulating io hematopoeitic progenitor cells and related products. In particular the invention includes methods and products for using CaR receptor-related compositions to enhance mobilization of hematopoietic progenitor cells, to improve the efficiency of targeting cells to the bone marrow, and/or to modulate hematopoietic progenitor cell function. | 12-27-2012 |
20130017600 | ISOLATION AND/OR PRESERVATION OF DENDRITIC CELLS FOR PROSTATE CANCER IMMUNOTHERAPY - Methods and compositions for use of human dendritic cells to activate T cells for immunotherapeutic responses against primary and metastatic prostate cancer are disclosed. In one embodiment, human dendritic cells, after exposure to a prostate cancer antigen or specific antigenic peptide, are administered to a prostate cancer patient to activate the relevant T cell responses in vivo. In an alternate embodiment, human dendritic cells are exposed to a prostate cancer antigen or specific antigenic peptide in vitro and incubated or cultured with primed or unprimed T cells to activate the relevant T cell responses in vitro. The activated T cells are then administered to a prostate cancer patient. Methods and compositions for human dendritic cells with extended life span and cryopreserved dendritic cells are disclosed. | 01-17-2013 |
20130034901 | Use of a Proteolytic Enzyme for the Modification of the Cell Surface of a Stem Cell - The present invention relates to a stem cell and/or a population thereof having a specific profile of cell surface proteins and/or proteoglycans. The present invention also relates to use of a proteolytic enzyme in the modification of the cell surface of a stem cell. The present invention further relates to a method of modifying the cell surface of a stem cell by treatment with a proteolytic enzyme. | 02-07-2013 |
20130084636 | SCAFFOLD FOR ARTICULAR CARTILAGE REGENERATION AND METHOD FOR MANUFACTURING SAME - Disclosed are scaffolds for regeneration of articular cartilage which are applicable to both the superficial zone and the middle zone of articular cartilage, and a method for manufacturing the same. The scaffolds have sufficient mechanical properties to support the implantation and regeneration of chondrocytes, and allow cells to show high cell viability with a high content of sulfated glycosaminoglycans (GAGs). In addition, being applicable to both the superficial zone and the middle zone of articular cartilage, the scaffolds facilitate cell adhesion and provide biomimetic surface environments that are effective for growing and differentiating stem cells. Therefore, the scaffolds are helpful in regenerating damaged articular cartilage, thus finding applications in stem cell therapy for articular cartilage damage and disease. Also, the application of the scaffolds can be extended to prostheses of the ear and the nose in plastic surgery. | 04-04-2013 |
20130164841 | METHOD FOR INDUCING HUMAN BLOOD-BORN HEMATOSPHERES THROUGH AGGREGATE CULTURE AND EXPANDING BLOOD ADULT STEM CELLS AND PROGENITOR CELLS, AND STEM CELL PREPARED BY THE SAME - The present invention relates to a technique of using monocytic blood cells to effectively culture and proliferate blood adult stem cells and progenitor cells that only exist in small quantities to effectively obtain large quantities of stem cells. According to the present invention, the limitation of being able to derive only small quantities of stem cells from blood can be overcome, and the pluripotency of stem cells can easily be obtained. | 06-27-2013 |
20130177982 | Interleukin-1 Alpha Antibodies and Methods of Use - Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors. | 07-11-2013 |
20130210141 | DIFFERENTIATION OF PLURIPOTENT CELLS - Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages. | 08-15-2013 |
20130236964 | METHODS, NUCLEIC ACID CONSTRUCTS AND CELLS FOR TREATING NEURODEGENERATIVE DISORDERS - A method of treating a neurodegenerative disorder is provided. The method is effected by administering to an individual in need thereof cells capable of exogenously regulatable neurotransmitter synthesis thereby treating the neurodegenerative disorder. | 09-12-2013 |
20130273653 | DIAGNOSTIC MARKER FOR MELANOMA, PHARMACEUTICAL COMPOSITION COMPRISING INHIBITOR OF THE FUNCTION OF THE HISTATIN-1 PROTEIN AND IMMUNOSUPPRESSOR - The present invention is directed to provide diagnostic markers for melanoma, novel pharmaceutical compositions, and novel immunosuppressors. The histatin-1 mRNA and the histatin-1 protein can be used as the diagnostic markers for melanoma. In addition an inhibitor of the function that inhibits the function of the histatin-1 protein may be used as a pharmaceutical composition. Furthermore, a dendritic cell differentiated from a monocyte by the action of the histatin-1 protein or the histatin-1 protein may be used as an immunosuppressor. | 10-17-2013 |
20130280805 | Dendritic Cell Compositions and Methods - Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells. | 10-24-2013 |
20130323834 | METHODS FOR INDUCING SELECTIVE APOPTOSIS - Provided herein are methods for cell therapy by modifying transfused cells to express an inducible caspase 9 protein, so that the cells may be selectively killed if the patient experiences dangerous side effects. Provided also within relates in part to methods for preventing or treating Graft versus Host Disease by modifying T cells before administration to a patient, so that they may be selectively killed if GvHD develops in the patient. | 12-05-2013 |
20140011276 | Formulation for Cultivating Dendritic Killer Cells and Method Using the Same - A formulation and method for cultivating dendritic killer cells is disclosed in the present invention. The formulation comprises an effective amount of at least one cytokine and the abovementioned cytokine is IL-15. Furthermore, the method for cultivating dendritic killer cells at least comprises the following steps. A peripheral blood mononuclear cell population is obtained from human blood at first. Effective amounts of the cytokines in the formulation mentioned above are then added into the peripheral blood mononuclear cell population and the abovementioned peripheral blood mononuclear cell population is placed for a first appropriate period. | 01-09-2014 |
20140017784 | Methods for Expansion and Analysis of Cultured Hematopoietic Stem Cells - Methods and kits for propagating hematopoietic stem cells are provided. The methods comprise culturing cells in medium comprising one or more angiopoietin-like proteins, under conditions sufficient for expansion of HSCs. Angiopoietin-like proteins include angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and microfibrillar-associated glycoprotein (Mfap4). Methods for identifying hematopoietic stem cells are provided and isolated hematopoietic stem cells are also provided. | 01-16-2014 |
20140120616 | RECOMBINANT HUMAN FIBRONECTIN FRAGMENT FOR CELL CULTURE - The present invention discloses protein fragment including a cell binding region of fibronectin and methods of using the same for cell culture. Additionally, surfaces modified with such protein fragment and compositions including the same are provided. Desirably, such surfaces allow various cells to adhere thereto yet avoid issues associated with animal-derived products. | 05-01-2014 |
20140242694 | COMPOSITION CONTAINING COMPLEX CYTOKINES DERIVED FROM EBV-INFECTED B CELLS FOR INDUCING THE MATURATION OF DENDRITIC CELLS - A composition for deriving the maturation of dendritic cells includes complex cytokines generated by the simulation, the expression of which is induced on EBV-infected B cells. The dendritic cell maturation process, which conventionally takes approximately 7 days, can be shortened to 2 days, thereby producing dendritic cells in a more economically advantageous and effective manner. | 08-28-2014 |
20140248699 | ERYTHROID CELLS PRODUCING ADULT-TYPE BETA-HEMOGLOBIN GENERATED FROM HUMAN EMBRYONIC STEM CELLS - Methods and compositions of erythroid cells that produce adult β-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions. | 09-04-2014 |
20140273210 | HIGH THROUGHPUT MECHANICAL STRAIN GENERATING SYSTEM FOR CELL CULTURES AND APPLICATIONS THEREOF - The present application relates to an adaptable cell culture system that allows the application of mechanical strain to cells in culture through the displacement of a stretchable cell culture substrate. The system can apply dynamically heterogeneous strains to simulate the complex in vivo strain profiles on cultured cells, the strains including simulation of physiologic waveform such as a simulation of normal or diseased physiological biphasic stretch of the cardiac cycle, a simulation of normal or diseased physiological arterial waveform, or a cyclic mechanical strain. The system is modular and compatible with commercially available cell culture plate formats and robotic liquid handling devices. The system has a variety of applications including screening compounds for cardio toxicity or therapeutic activity, and identifying drug target, all using cell culture under the mechanical strain applied by the system. | 09-18-2014 |
20140273211 | Methods and Materials for Hematoendothelial Differentiation of Human Pluripotent Stem Cells Under Defined Conditions - Methods and compositions for differentiating pluripotent stem cells into cells of endothelial and hematopoietic lineages are disclosed. | 09-18-2014 |
20140377863 | SYNTHETIC SCAFFOLDS AND ORGAN AND TISSUE TRANSPLANTATION - A synthetic scaffold for replacing at least a portion of an airway includes an airway mold, one or more structural ribs on the airway mold, and a non-structural wall. Each of the one or more structural ribs is formed from a first material and the non-structural wall is formed from a second material. The non-structural wall coats the airway mold and forms a conduit that incorporates the one or more structural ribs. | 12-25-2014 |
20150072419 | NOVEL CARBOHYDRATE PROFILE COMPOSITIONS FROM HUMAN CELLS AND METHODS FOR ANALYSIS AND MODIFICATION THEREOF - The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces. | 03-12-2015 |
20150072420 | THERAPEUTIC USE OF CD31 EXPRESSING CELLS - As described below, the present invention features compositions and methods related to the isolation, culture and therapeutic use of CD31-expressing cells. | 03-12-2015 |
20150072421 | METHOD FOR MANUFACTURING STEM CELL HAVING APPROPRIATE SIZE FOR INTRAVASCULAR ADMINISTRATION - The present invention relates to a method for preparing stem cells having a size suitable for intravascular administration, and preferably to a method for preparing stem cells with a diameter ranging from 11-16 μm. Additionally, the present invention relates to media composition for preparing stem cells having a size suitable for intravascular administration. According to the present invention, stem cells having a size suitable for intravascular administration can be prepared such that stem cells administered into a vein can stably reach a target tissue, and thus can more efficiently increase efficacy displaying activity, thereby innovatively enhancing the efficacy of cell therapy using stem cells. | 03-12-2015 |
20150079677 | METHODS AND COMPOSITIONS FOR SEPARATING RARE CELLS FROM FLUID SAMPLES - The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components. | 03-19-2015 |
20150087059 | HIV VPR-SPECIFIC T-CELL RECEPTORS - The instant invention provides TCRs having one or more amino acid substitutions that bind to the AL9 epitope of the HIV protein vpr (AIIRILQQQL). | 03-26-2015 |
20150111296 | Transcription Factor Mediated Programming Towards Megakaryocytes - This invention relates to the forward programming of pluripotent stem cells (PSCs) into megakaryocyte (MK) progenitor cells using the transcription factors GATA1, FLI1 and TAL1. Methods of producing megakaryocyte (MK) progenitor cells and subsequently differentiating them into mature megakaryocytes are provided. | 04-23-2015 |
20150307843 | METHOD TO OBTAIN HEMATOPOIETIC STEM CELLS USING THREE-DIMENSIONAL INDUCING SYSTEM - The invention relates to a method of acquiring hematopoietic stem cells (HSCs) by means of three dimensional inducing system with high efficiency—which makes use of three-dimensional cell culture matrix or cell culture scaffolds, such as three-dimensional cell culture system which is made from hydrogel, seaweed and other materials, and/or associating stromal-cell including bone marrow cell, mouse bone marrow cell line OP9, OP9DL1 and so on, and/or associating multiple factors including mesoderm inducible factor, hematopoietic growth factor etc which can induce pluripotent stem cells to differentiate into hematopoietic stem cells. The invention sets up a new method of acquiring hemopoietic stem cells, establishing a system that induces pluripotent stem cells to differentiate into hemopoietic stem cells with high efficiency by means of utilizing dimensional inducing system and/or associating stromal-cell including bone marrow cell and/or multiple factors for the first time, which provide a theoretical basis and technology platform for acquiring clinical available hematopoietic stem cells, and expand additional methodologies and ideas in fields of disease mechanism exploring, drug screening etc for hematopoietic cells from pluripotent stem cells. | 10-29-2015 |
20160010059 | EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS BY HISTONE METHYLTRANSFERASE G9a INHIBITION | 01-14-2016 |
20160024472 | Generation of Dendritic Cells from Monocytic Dendritic Precursor Cells with GM-CSF in the Absence of Additional Cytokines - The present invention it was determined that dendritic cells could be derived from various sources including peripheral blood monocytes in the presence of only GM-CSF without other cytokines if the monocytes were not activated. By preventing activation, such as by preventing binding of the cells to the surface of the culture vessel, the monocytes do not require the presence of additional cytokines, such as IL-4 or IL-13, to prevent differentiation into a non-dendritic cell lineage. The immature DCs generated and maintained in this manner were CD14 and expressed high levels of CD1a. Upon maturation by contact with an agent such as, for example, BCG and IFNγ, the cells were determined to express surface molecules typical of mature dendritic cells purified by prior methods and cultured in the presence of GM-CSF and IL-4. The mature dendritic cells produced from monocytes without activation and cultured in GM-CSF alone are suitable for use in dendritic cell-based immunotherapy methods, such as for use in the treatment of disease, including cancer. | 01-28-2016 |
20160053224 | METHODS OF CELL SEPARATION - The present invention relates to the use of a combination of: (i)a macromolecular erythrocyte sedimentation enhancer, and (ii) dimethyl sulphoxide (DMSO), dimethylglycine (DMG) and/or valine; to recover non-erythrocyte blood cells from a blood cell-containing sample and/or to prime non-erythrocyte blood cells to protect their integrity in subsequent cryopreservation step(s). | 02-25-2016 |
20160068815 | METHODS AND APPARATUSES FOR UMBILICAL CORD BLOOD COLLECTION AND ISOLATION OF CELLS - The invention relates to method for the separation of cord blood endothelial cells from hematopoietic cells in a manner that preserves the hematopoietic cells' repopulating ability and primitivity, without significantly reducing the yield of hematopoietic cells. The said endothelial cells from cord blood have a utility in cell based therapeutics. The system described takes advantage of the endogenous adherence properties of the cells to facilitate the separation and separate storage of the cell types. | 03-10-2016 |
20160090569 | Scheduled Feed - Embodiments described herein generally provide for the scheduled feeding of cells in a cell expansion system. A schedule to proactively and automatically increase and/or maintain inlet rates of media to feed cells may be created, in which inlet rates to the intracapillary portion (or extracapillary portion) of a bioreactor may be increased or maintained according to the schedule. Such schedule may be conservative or aggressive or a combination thereof, for example. Multiple schedules may be used. Scheduled media exchanges may also be included. By following a feed schedule, the monitoring of metabolite levels may be optional. Inlet rates may be increased or maintained without manual manipulation. Media usage may also be more predictable. In an embodiment, a custom task(s) may be created to follow a desired feed schedule. In another embodiment, a pre-programmed task(s) may be used for the scheduled feeding of cells. | 03-31-2016 |