Class / Patent application number | Description | Number of patent applications / Date published |
435173700 | Lytic effect produced (e.g., disruption of cell membrane for release of subcellular parts; e.g., nucleic acids, etc.) | 32 |
20090047722 | SYSTEMS, DEVICES, AND METHODS FOR BIOMASS PRODUCTION - Systems, devices, and methods for releasing one or more cell components from a photosynthetic organism. A bioreactor system is operable for growing photosynthetic organisms. Some of the methods include contacting the photosynthetic organism with an energy-activatable sensitizer, and activating the energy-activatable sensitizer, thereby releasing a cellular component from at least one of, for example, a membrane structure, tubule, vesicle, cisterna, organelle, cell compartment, plastid, or mitochondrion, associated with the photosynthetic organisms. | 02-19-2009 |
20090081751 | NATIVELY GLYCOSYLATED MAMMALIAN BIOLOGICAL MOLECULES PRODUCED BY ELECTROMAGNETICALLY STIMULATING LIVING MAMMALIAN CELLS - A composition is disclosed with the composition comprising a mixture of natively glycosylated mammalian biological molecules produced by electromagnetically stimulating living mammalian cells. | 03-26-2009 |
20090093036 | METHOD FOR RELEASING GENETIC MATERIAL FROM SOLID PHASE - The present invention relates to systems for releasing genetic materials from a solid medium. The present invention also relates to methods for releasing genetic materials from a solid medium. The present invention further relates to methods for isolating genetic material from a biological sample. | 04-09-2009 |
20090155877 | Biochip for sorting and lysing biological samples - A biochip ( | 06-18-2009 |
20090317884 | METHOD AND APPARATUS FOR TREATMENT ENHANCEMENT IN ACOUSTIC PROCESSING OF SAMPLES - Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using an energy director positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur. | 12-24-2009 |
20100028969 | CELL LYSIS OR ELECTROPORATION DEVICE COMPRISING AT LEAST ONE PYROELECTRIC MATERIAL - The present invention relates to a cell lysis or electroporation device comprising at least one pyroelectric material. | 02-04-2010 |
20100035322 | NON INVASIVE METHOD OF ELECTROPORATION MEDIATED BY CARBON NANOTUBES AND DEVICE FOR PUTTING THE METHOD INTO PRACTICE - A non-invasive method for the electroporation of cells contained in a substrate wherein the cells are placed in contact with carbon nanotubes and then said substrate is subjected to the action of two orthogonal pulsed electric fields generated by means of two pairs of electrodes in contact with said substrate according to a specific time sequence in such a way that, when a pair of electrodes is active, the other pair of electrodes is deactivated and vice versa. A device for performing electroporation according to this method. | 02-11-2010 |
20100068781 | MECHANICAL LYSIS ARRANGEMENTS AND METHODS - Methods and arrangements to lyse a biological sample are described. The arrangements comprise a lysis tube containing the sample, one or more electromagnets generating a magnetic field, and one or more permanent magnets inside the lysis tube. The permanent magnets move and lyse the sample when a magnetic field is generated by the electromagnets. | 03-18-2010 |
20100151553 | METHOD OF DETECTION OF BIOANALYTES BY ACOUSTO-MECHANICAL DETECTION SYSTEMS COMPRISING THE ADDITION OF LIPOSOMES - Methods for detecting target biological analytes within sample material using acousto-mechanical energy generated by a sensor are disclosed. The acousto-mechanical energy may be provided using an acousto-mechanical sensor, e.g., a surface acoustic wave sensor such as, e.g., a shear horizontal surface acoustic wave sensor (e.g., a LSH-SAW sensor). The detection of the target biological analytes in sample material are enhanced by contacting the target biological analyte and/or the sensor surface with liposomes that amplify the sensor sensitivity by (1) modifying the rheological properties of the fluid near the sensor surface; (2) changing the mass attached to the surface; and/or (3) modifying the dielectric properties of the fluid near the sensor surface, the sensor surface itself and/or any intervening layers on the sensor surface. | 06-17-2010 |
20100159556 | Method for Manipulating Magnetic Particles in a Liquid Medium - A method of mixing magnetic particles ( | 06-24-2010 |
20110217748 | Algal Cell Lysis And Lipid Extraction Using Electromagnetic Radiation-Excitable Metallic Nanoparticles - According to one embodiment, the disclosure provides a system for algal cell lysis. The system may include at least one algal cell, a plurality of metal nanoparticles, and an electromagnetic radiation generator. The generator may be able to generate radio frequency or microwave radiation that excites the plurality of metal nanoparticles, resulting in lysis of the algal cell. The disclosure also provides a system for recovery of a lipid from an algal cell similar to the above system but also including a separator. The disclosure further provides a method of recovering a lipid from an algal cell by supplying a plurality of metal nanoparticles to at least one algal cell, exciting the plurality of metal nanoparticles with radio frequency or microwave electromagnetic radiation, lysing the algal cell to release the lipid, and separating the lipid from the lysed algal cell. | 09-08-2011 |
20110275132 | PURIFICATION OF BACTERIAL ANTIGENS - Presented are methods of isolation of pili and pilus-like structures from Gram-positive bacteria including | 11-10-2011 |
20110306109 | BARRIERS FOR FACILITATING BIOLOGICAL REACTIONS - The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes. | 12-15-2011 |
20120015419 | METHOD AND APPARATUS FOR HEADSPACE CONTROL IN ACOUSTIC PROCESSING OF SAMPLES - Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace size at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using a headspace control element positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur. | 01-19-2012 |
20120021481 | ELECTROMECHANICAL LYSING OF ALGAE CELLS - Methods and electroporation devices for electrical treatment of algal cell cultures for release of lipids and proteins are described herein. The method of the present invention exploits the differences in electrical time constants for the media inside the cell and outside the cell to produce a net force to cause cellular lysis and extract cellular components. The method of the present invention can be used in the treatment of flocculated as well as unflocculated algal cell cultures. The device of the present invention provides efficient cell lysing in a low-energy cost set-up. | 01-26-2012 |
20120094353 | Low-Biomass Soil DNA/RNA Extraction Yield and Quality - The present application discloses a method for pre-treating a sample of a DNA/RNA adsorbing matrix (e.g. soil) suitable for extracting cell-derived (deoxy) ribonucleic acid therefrom, comprising the steps of: isolating a sample of a DNA/RNA adsorbing matrix, said sample comprising DNA/RNA-containing cells; and bringing the sample into contact with DNA of natural origin in a fragmented form (e.g. salmon sperm DNA) so as to essentially block said DNA/RNA adsorbing binding sites of said adsorbing matrix, said fragmented DNA being “unrelated” to the target DNA/RNA of said DNA/RNA-containing cells. The fragmented DNA of natural origin can be depurinated and/or freeze-dried. The application further discloses a method for extracting DNA/RNA from a sample and a kit useful for pre-treating samples. | 04-19-2012 |
20120252087 | Bipolar Flyback Power Supply - A device, system and method for treating biological cells includes a voltage source, a half-controlled bridge connected to the voltage source, and a load connected across the half-controlled bridge. The half-controlled bridge includes a first switch, a second switch, a first diode and a second diode. The load includes an inductor connected in parallel with a cell or chamber. A controller is connected to the first and second switches and operates the first switch and the second switch to selectively generate one or more bipolar pulses, wherein each bipolar pulse comprises a positive polarity voltage pulse and a negative polarity voltage pulse with a negligible delay between the positive polarity voltage pulse and the negative polarity voltage pulse. | 10-04-2012 |
20120322132 | CENTRIFUGAL FORCE-BASED MICROFLUIDIC DEVICE FOR NUCLEIC ACID EXTRACTION AND MICROFLUIDIC SYSTEM INCLUDING THE MICROFLUIDIC DEVICE - A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves. | 12-20-2012 |
20120329122 | ULTRASOUND AND ACOUSTOPHORESIS FOR COLLECTION AND PROCESSING OF OLEAGINOUS MICROORGANISMS - Microorganisms such as microalgae are collected and separated from a host medium such as water. Cellular walls and membranes of the microorganisms are then ruptured to release their lipids using a lipid extraction unit. Thereafter, the lipids from the host medium are collected and separated using a lipid collection and separation unit. Related apparatus, systems, techniques and articles are also described. | 12-27-2012 |
20130102047 | OIL-BASED MATERIAL-PRODUCING METHOD AND OIL-BASED MATERIAL-PRODUCING APPARATUS - An oil-based material-producing method includes a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves. The oil-based material-producing method may also include a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step. | 04-25-2013 |
20130115670 | CELL DISRUPTION - A process for the disruption of a biological cell comprising freezing, boiling or perhaps alternately freezing and boiling the material containing the biological cell using a thermoelectric cell a base face whereof is contiguous with a heat sink/source held at a substantially constant temperature and a working face . Apparatus for carrying out the disruption process comprises a peltier cell a base face of which is flexibly attached to a heat sink arranged to be kept at a constant temperature of around 50° C. and a working face of which is contiguous with a reaction vessel or a reaction vessel holder. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel holder for heating with the TEC used purely for cooling The peltier cell base face is constructed of materials which tend to inhibit disintegration of the peltier cell brought about by expansion and contraction under heat. | 05-09-2013 |
20140065687 | Bioreactor System and Related Bio-Stimulation Methods - A bioreactor system for growing commercial volumes of algae or other biomass in an enclosed, biosecure reactor vessel, the system having internal artificial growth light production as well as exterior solar energy capturing devices or the like designed to facilitate enhanced sunlight exposure for photosynthesis organism production. Magnetic and electromagnetic field generation systems and/or millimeter wave generating devices are integrated with the bioreactor system and its operation to substantially enhance growth rate and overall productivity. | 03-06-2014 |
20140120599 | DEVICES, METHOD AND SYSTEM EMPLOYABLE FOR LYSING - A device configured for lysing a sample includes a holding element configured to hold an energy-transmitting element such that the holding element lies around the held part of the energy-transmitting element and envelops the held part of the energy-transmitting element and/or that the holding element lies against the held part of the energy-transmitting element. The holding element is further configured to cause lysis with the energy-transmitting element when the holding element is immersed into the sample to be subjected to lysis or comes into contact with the sample to be subjected to lysis. A method includes inserting the energy-transmitting element into the holding element. A system configured for lysing a sample by way of ultrasound includes the device and the energy transmitting element. | 05-01-2014 |
20140147903 | Process for the Preparation of Amoebocyte Lysate from Haemolymph of the Horseshoe Crab - The present invention relates to a non-destructive process for the preparation of amoebocyte lysate from the haemolymph of horseshoe crab, characterized by the steps of firstly, means for acclimatizing a live specimen of horseshoe crab in treated seawater under controlled conditions; then, means for cleaning the live specimen, including cleaning the base of the last pair of thoracic appendages of the live specimen; next, means for withdrawing the haemolymph through the base of the last pair of thoracic appendage under aseptic condition; then, means for transferring the collected haemolymph into a pre-cooled disposable centrifuge tube; then, means for centrifuging the collected haemolymph to separate amoebocyte cells from lymph; then, means for decanting the lymph out of the disposable centrifuge tube; then, means for washing the white amoebocyte cells and centrifuging; then, means for sonicating the amoebocyte cells for lysing of the amoebocyte cells; then, means for storing the sonicated amoebocyte cells for further lysing of the amoebocyte cells; then, means for centrifuging the sonicated mixture, thereby separating the amoebocyte lysate in the form of a supernatant; then, means for decanting the amoebocyte lysate into a sterilized vessel; then, means for pre-freezing the amoebocyte lysate; and subsequently, freeze-drying the amoebocyte lysate. | 05-29-2014 |
20140199744 | ALGAL CELL LYSIS AND LIPID EXTRACTION USING ELECTROMAGNETIC RADIATION-EXCITABLE METALLIC NANOPARTICLES - According to one embodiment, the disclosure provides a system for algal cell lysis. The system may include at least one algal cell, a plurality of metal nanoparticles, and an electromagnetic radiation generator. The generator may be able to generate radio frequency or microwave radiation that excites the plurality of metal nanoparticles, resulting in lysis of the algal cell. The disclosure also provides a system for recovery of a lipid from an algal cell similar to the above system but also including a separator. The disclosure further provides a method of recovering a lipid from an algal cell by supplying a plurality of metal nanoparticles to at least one algal cell, exciting the plurality of metal nanoparticles with radio frequency or microwave electromagnetic radiation, lysing the algal cell to release the lipid, and separating the lipid from the lysed algal cell. | 07-17-2014 |
20140206060 | CONTINUOUS SONICATION FOR BIOTECHNOLOGY APPLICATIONS AND BIOFUEL PRODUCTION - A system and method for sonication of multiple samples and continuous sonication of an input fluid stream in flow-through arrangements useful for economical breakdown of particulates and organisms present in large volumes with relatively low-power sonication devices such as production of oil from algae. The system includes an electrical wave generator oscillating in the ultrasound range, a vibrating element electrically connected to the electrical wave generator, and a sonication plate that vibrates in certain modes. The sonication plate contains features for mating with sample tubes, and the sample tubes also possess complimentary mating features to those on the sonication plate. A method for sonication of multiple samples includes utilizing mating features to attach tubes to the sonication plate and energizing the sonicator to vibrate the sonication plate. The invention also relates to arrangements for continuous flow-through useful for sonicating large sample volumes. | 07-24-2014 |
20150079655 | METHOD AND APPARATUS FOR PROCESSING SAMPLE MATERIAL - Methods and systems for treating material with focused acoustic energy and/or ultraviolet radiation are described. Sample material may be provided to a single batch vessel or may flow in a continuous or intermittent fashion into/out of a processing chamber within which the material is exposed to focused acoustic energy and/or ultraviolet radiation. Exposure to focused acoustics in a flow through environment may enhance the effects of the radiation on the sample material. Further, in flow-through or single batch arrangements, sample material may be retained exterior to or within a processing chamber by one or more retaining members (e.g., filter, valve, etc.) until a characteristic of the sample material reaches one or more particular criteria (e.g., physical, chemical, biological). Once the criteria of the sample material is attained (e.g., via further treatment), the material is then permitted to flow past the retaining member(s) and through the inlet or outlet of the chamber. Further, focused acoustic processing may be used to treat articles, such as the retaining member(s), rather than only sample material located within an internal processing volume of the chamber. | 03-19-2015 |
20150307809 | SYSTEMS AND METHODS FOR EXTRACTING NON-POLAR LIPIDS FROM AN AQUEOUS ALGAE SLURRY AND LIPIDS PRODUCED THEREFROM - Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0 5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll. | 10-29-2015 |
20160010025 | Fractionation of Oil-Bearing Microbial Biomass | 01-14-2016 |
20160038932 | METHODS AND APPARATUSES FOR ACOUSTIC TREATMENT OF SAMPLES - A treatment vessel may allow a user, or automated system, to manipulate sample material within a treatment area during processing (e.g., focused acoustic treatment), as well as subject the sample material to a staged processing protocol. The vessel may include openings for receiving/discharging the sample material. Walls within the vessel may be movable between various positions, to permit or obstruct flow of sample material into or out from a treatment area. The wall(s) may push the sample material within the vessel, as well as adjust pressure levels within the treatment area. In some embodiments, an acoustic treatment system may include a flexible coupling medium that may be deformed toward the vessel upon an application of suitable pressure thereto. When the medium presses up against the vessel, defects (e.g., particles, bubbles, interfaces, etc.) that may otherwise be present along the acoustic wave path may be reduced. | 02-11-2016 |
20160101299 | SELECTIVE REMOVAL OF CELLS HAVING ACCUMULATED AGENTS - A method of treating a condition associate with accumulation of an agent in cells in a patient includes exposing the cells to ultrasound, to selectively kill or induce apoptosis in the cells. The cells include the accumulated agent. | 04-14-2016 |
20160177291 | Method and Device for Preparing a Sample of Biological Material Containing Target Cells and Accompanying Cells for Extracting Nucleic Acids of the Target Cells | 06-23-2016 |