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Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.)

Subclass of:

435 - Chemistry: molecular biology and microbiology

435440000 - PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION

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Class / Patent application numberDescriptionNumber of patent applications / Date published
435476000 The polynucleotide is a plasmid or episome 28
435475000 The polynucleotide is unencapsidated bacteriophage or viral nucleic acid 1
20110244576Herpes Simplex Virus Complex - There is provided an HSV complex which comprises an avirulent HSV and a targeting agent which allows the HSV particle to infect and lyse a specific targeted cell. The inventors have found a way in which avirulent HSV can be targeted to disease cells, e.g. cancer cells, by incorporating an antibody binding domain into one or more viral glycoproteins.10-06-2011
435490000 The polynucleotide is an unbranched linear fragment 1
20110014709Method of Modifying Target Region in Host DNA and Selectable Marker Cassette - A method of modifying a target region in a host DNA using a donor DNA: 01-20-2011
Entries
DocumentTitleDate
20090291504CRE COMPLEMENTATION USING LEUCINE ZIPPER - Cre activity is effectively reconstituted from two modified, inactive Cre fragments, both in cell culture and in transgenic mice. Cre reassociation was facilitated by fusing fragments separately to peptides that can form a tight anti-parallel leucine zipper. The co-expressed Cre fusion fragments showed substantial activity in cultured cells. Also disclosed are in vivo techniques for increasing the cell-specificity of gene manipulation, where expression in transgenic mice using individual promoters for each Cre fragment effectively reconstituted Cre activity, and the activation of LoxP recombination in the co-expressing cells.11-26-2009
20110195506METHOD FOR PRODUCING YEAST EXPRESSED HPV TYPES 6 AND 16 CAPSID PROTEINS - Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production of conformational antibodies against both L1 proteins upon administration to mice.08-11-2011
20090155912METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS - The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.06-18-2009
20090155913COMPOSITIONS COMPRISING PROMOTER SEQUENCES AND METHODS OF USE - Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided.06-18-2009
20100041153Expression of Proteins in E. Coli - Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX1(X 2X 3) n X 1 represents K or R; X 2 represents M, S or T; X 3 represents K or R; n represents an integer of 1 or larger; and wherein said DNA is operably-linked to a promoter sequence are provided.02-18-2010
20100144041 L-Arabinose Fermenting Yeast - An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.06-10-2010
20130029426Dual Inducible System for the cSPP System - The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids.01-31-2013
20090042301TEMPERATURE REGULATED GENE EXPRESSION - A method for regulation of gene expression by variation of temperature uses a riboswitch. The riboswitch includes a 5′-UTR construct of crhC which alters its secondary structure in response to temperature, resulting in a more stable transcript at lower temperatures, permitting translation. At higher temperatures, the transcript is destabilized and functionally inactive. The 5′-UTR construct of crhC may be operatively linked to a promoter and a gene and administered to cells with an expression vector.02-12-2009
20090305421Recombinant vector for deleting specific regions of chromosome and method for deleting specific chromosomal regions of chromosome in the microorganism using the same - Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (λ)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.12-10-2009
20130059389Method for Producing Kluyveromyces Marxianus Transformant - An object to be solved by the present invention is to provide, for example, a method for producing a 03-07-2013
20110014708NUCLEIC ACID FOR USE IN ALGAE AND USE THEREOF - The present invention provides a modified nucleic acid for expressing 01-20-2011
20090269852Novel gene gms 01 - The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.10-29-2009
20130065312METHOD AND COMPOSITION FOR GENERATING PROGRAMMED CELL DEATH RESISTANT ALGAL CELLS - The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation.03-14-2013
20130065314ALGAL TRANSFORMATION SYSTEMS, COMPOSITIONS AND METHODS - One aspect of the invention relates to a method for introducing a cargo molecule into an algal cell, the method comprising preparing a composition comprising the cargo molecule and a cell penetrating peptide (CPP) and exposing the algal cell to the composition. The method may also comprise treating the algal cell to disrupt a cell wall of the algal cell. One aspect of the invention relates to a method for transforming an algal cell, the method comprising preparing a composition comprising a nucleic acid molecule and a cell penetrating peptide and exposing the algal cell to the composition. In some embodiments, the CPP is Transportan, Penetratin, an HIV Tat fragment, or a polyarginine, or a variant thereof.03-14-2013
20130065313MOLECULAR BIOLOGY TOOLS FOR ALGAL ENGINEERING - The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.03-14-2013
20110020936METHOD FOR ELECTROPORATION OF LACTOBACILLUS BUCHNERI WITH NUCLEIC ACID - Electroporation methods for transferring a nucleic acid of interest, including ferulic acid esterase nucleic acid, into bacterial host cells, such as 01-27-2011
20090130763Method of Controlling Degradation of Protein by Tetracycline Antibiotic - The present invention provides a fusion protein comprising a variant protein of a protein binding to an antibiotic and a target protein having fused thereto, wherein the variant protein is degraded when the antibiotic is not bound but stabilized when the antibiotic is bound in a cell, and the fusion protein is degraded when the antibiotic is not bound but stabilized when the antibiotic is bound in a cell.05-21-2009
20100285592SINGLE EXPRESSION VECTOR FOR GENERATION OF A VIRUS WITH A SEGMENTED GENOME - The present invention encompasses an expression vector that is capable of generating a virus from a segmented genome. In particular, a single expression vector may be utilized to produce influenza virus in cultured cells. The expression vector can be delivered in a purified DNA form or by a suitably designed bacterial carrier to cells in culture or to animals. This invention increases the virus generation efficiency, which benefits vaccine development. The bacterial carrier harboring such a plasmid encoding an attenuated virus may be used as a vaccine against corresponding viral disease.11-11-2010
20110300633NOVEL PROMOTER FOR USE IN TRANSFORMATION OF ALGAE - The objective of the present invention is to provide a transformation method that is applicable to a wide variety of species of algae with high efficiency. The promoter of the present invention is characterized in containing a polynucleotide constituting a non-coding region located upstream from a gene encoding a replication-associated protein of a CdebDNA virus or the like.12-08-2011
20110281362Electrotransformation of Gram-Positive, Anaerobic, Thermophilic Bacteria - The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria.11-17-2011
20090215179Transgenically preventing establishment and spread of transgenic algae in natural ecosystems - Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species. as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems.08-27-2009
20100035346SINGLE PROTEIN PRODUCTION IN LIVING CELLS FACILITATED BY A MESSENGER RNA INTERFERASE - The present invention describes a single-protein production (SPP) system in living 02-11-2010
20100267147SITE-DIRECTED MUTAGENESIS IN CIRCULAR METHYLATED DNA - Site-specific mutation in methylated circular stranded DNA molecules is conferred by mutagenic primer pairs and methylase deficient 10-21-2010
20120034698COMPOSITIONS AND METHODS FOR INCREASING OIL CONTENT IN ALGAE - The present invention provides methods and compositions for increasing oil content in algae. More particularly, the present invention relates to enhancement of phytohormone activity in algae, by genetic transformation thereof or by supplementing the algal growth medium with phytohormones, to maximize the production of oil within the algae cells.02-09-2012
20110195505BACTERIAL STRAINS FOR BUTANOL PRODUCTION - Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.08-11-2011
20120295356APPARATUS AND METHOD FOR GENETICALLY TRANSFORMING CELLS - A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material.11-22-2012
20080206873COMPLEX - A complex comprising a double-stranded DNA containing a terminal repeated sequence originating in a retrovirus and a DNA having a base sequence which does not occur in this retrovirus and a retrovirus-origin protein component.08-28-2008
20090280569Chloramphenicol Resistance Selection in Bacillus Licheniformis - The present invention relates to a modified 11-12-2009
20080286871Modular genomes for synthetic biology and metabolic engineering - The invention provides methods and compositions for assembling a modular replacement genome in a host microorganism. After such assembly, the host organism's genome is inactivated or ablated to permit full control of host cellular functions by the replacement genome. A modular replacement genome comprises an assembly of nucleic acid fragments, or segments, derived from one or more natural organisms or from synthetic polynucleotides or from a combination of both. Such an assembly, or set, of segments making up a replacement genome comprises a substantially complete set of genes and regulatory elements for carrying out minimal life functions under predefined culture conditions. The invention provides modular genomes having modules that are amenable to facile replacement, deletion, and/or additions. Such modules may be synthetic polynucleotides and may be designed for controlling gene content, excluding of genes that encode inhibitors or otherwise undesirable competing enzymes that divert a host cell from desired metabolic/synthetic processes; modifying codon usage to maximize or minimize protein production; modifying regulatory elements, including promoters, enhancers, repressors, activator, or the like, to modulate gene expression; balancing enzymatic and transport activities to optimize fluxes of substrates, intermediates, and products in metabolic pathways, and like objectives.11-20-2008
20130023053METHODS AND COMPOSITIONS FOR TARGETED MUTAGENESIS IN BACTERIA - This disclosure provides methods and compositions for targeted mutagenesis of specific genes in a bacterial strain. By inducibly over-expressing error-prone polymerases such as Pol IV or Pol V in conjunction with nickase in a bacterial strain, and housing the targeted gene(s) on an episome or plasmid which contains one or more nickase recognition sequences, the targeted gene(s) can be selectively mutated at rates significantly greater than genes contained on the chromosome. The methods disclosed herein are useful for engineering desirable bacterial phenotypes and novel strains, including for example strains useful for treating or degrading waste and/or environmental contaminants, for optimizing bioprocesses, and for converting low-value feed-stock into value-added products.01-24-2013
20090186415METHOD FOR PRODUCTION OF CYTOCHROME P450 WITH N-TERMINAL TRUNCATED P450 REDUCTASE - The present invention provides a P450 reductase lacking N-terminal amino acids, as well as a nucleic acid encoding the P450 reductase. When co-expressed with a cytochrome P450, the P450 reductase increases the activity and/or expression of the cytochrome P450 compared to the activity and/or expression of the cytochrome P450 when co-expressed with a wild type P450 reductase. The invention also provides the use of certain promoters to increase expression of cytochrome P450, P450 reductase and/or b5, as well as the use of protease-deficient strains of yeast in which to express the proteins of the present invention.07-23-2009
20080318321Methods for Generating Hypermutable Microbes - Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.12-25-2008
20090325298Pro-Apoptotic Bacteria and Compositions for Delivery and Expression of Antigens - Whole-cell vaccines and methods for enhancing the immunogenicity of cellular microorganisms for use in producing protective immune responses in vertebrate hosts subsequently exposed to pathogenic bacteria or for use as vectors to express exogenous antigens and induce responses against other infectious agents or cancer cells. The present invention involves an additional method of enhancing antigen presentation by intracellular bacteria in a manner that improves vaccine efficacy. After identifying an enzyme that has an anti-apoptotic effect upon host cells infected by an intracellular microbe, the activity of the enzyme produced by the intracellular microbe is reduced by expressing a mutant copy of the enzyme, thereby modifying the microbe so that it increases immunogenicity.12-31-2009
20090081793Method for improving acid and low pH tolerance in yeast - A method for increasing tolerance in yeast to organic acids and low pH comprising functionally transforming a yeast with at least one copy of a nucleotide sequence encoding a plasma membrane H03-26-2009
20090221078ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE - Through screening of a 09-03-2009
20090246876HIGH EXPRESSION ZYMOMONAS PROMOTERS - Identified are mutants of the promoter of the 10-01-2009
20120196372Methods for Reducing Gluconoylation of Proteins - The present invention relates to methods of preventing glyconoylation of polypeptides produced in micororganisms.08-02-2012
20130217132METHODS, COMPOSITIONS AND USE FOR ENHANCING CHEMICAL TOLERANCE BY MICROORGANISMS - Embodiments herein concern compositions and methods for enhancing chemical tolerance of biomass conversion by microorganisms. In some embodiments, enhancing tolerance of biomass hydrolysate conversion includes enhancing tolerance to low molecular weight organic compounds.08-22-2013
20120129264Method for Transforming a Bacterium Belonging to the Streptococcus Genus by Natural Competence - The present invention relates to peptides, nucleic acids and methods for transforming a bacterium belonging to the 05-24-2012
20090042302Isolated Polynucleotides and Methods of Promoting a Morphology in a Fungus - The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.02-12-2009
20130137181Ethanol-Resistant Yeast Gene, and Use Thereof - The present invention relates to a gene associated with ethanol tolerance, and yeast strains and uses using the same. The yeast strain of this invention may growth under the condition not only with high-concentration ethanol, preferably 6-15% ethanol, but also in high osmotic pressure, preferably 30-40% glucose or sucrose. The present inventors developed yeast strains resistant to high-concentration glucose and ethanol, suggesting that they would be valuably applied to much effective ethanol production, and also be utilized as a superbacteria having tolerance to various stresses for ethanol production with high efficiency.05-30-2013
20130130389NOVEL PROMOTER FOR USE IN TRANSFORMATION OF ALGAE - The problem to be solved by the present invention is to provide a highly-efficient transformation technology, specifically, a highly-efficient promoter used for transforming algae, a vector comprising the promoter, and a method for transforming algae by using the vector. The promoter according to the present invention is characterized in comprising a polynucleotide constituting a non-coding region located upstream of a gene encoding a structural protein of a ClorDNA virus, and the like.05-23-2013
20110020937FLOCCULENT YEAST AND METHOD FOR PRODUCTION THEREOF - It is to provide a novel 01-27-2011
20110033938SYSTEM FOR INDUCIBLE GENE EXPRESSION IN CHLAMYDOMONAS - The unicellular green alga 02-10-2011
20110244575METHODS, SYSTEMS AND COMPOSITIONS FOR INCREASED MICROORGANISM TOLERANCE TO AND PRODUCTION OF 3-HYDROXYPROPIONIC ACID (3-HP) - The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex (“3HPTGC”). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made to provide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC.10-06-2011
20120244622REGULATED GENE EXPRESSION SYSTEMS AND CONSTRUCTS THEREOF - Compositions and methods for nitrogen sensitive regulation of expression of a transcribable nucleic acid molecule. One aspect provides a nitrogen-sensitive expression system that includes a transcription factor region comprising an NtcA binding site and a core promoter region comprising a RuBisCo promoter or a variant or a functional fragment thereof. Another aspect provides a method of transforming a host cell with an expression system. Also provided are expression cassettes, transformed host cells, and kits.09-27-2012
20120244621TUMOR-SPECIFIC BACTERIAL PROMOTER ELEMENTS - The invention relates to bacterial promoter elements which constitute or which are comprised in promoter regions and which confer tumour specificity to the promoter region activity, resulting in transcription of a transgene, which can be a heterologous or a homologous gene, which transgene is functionally arranged downstream of the promoter region at presence of a host bacterium in tumour tissue, while essentially conferring inactivity of the promoter region and no transcription at presence of the host bacterium in non-tumour tissue. Accordingly, the invention relates to bacterial promoter regions containing these tumour specific promoter elements.09-27-2012
20120190116PROCESS FOR CHROMOSOMAL INTEGRATION AND DNA SEQUENCE REPLACEMENT IN CLOSTRIDIA - The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.07-26-2012
20120190115Genes for Enhanced Lipid Metabolism for Accumulation of Lipids - Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene.07-26-2012
20090035863Method for the deletion of a large chromosomal region - In order to efficiently delete a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding a toxic substance such as Aflatoxin, the present invention provides a method for the deletion of a large chromosomal region comprising transforming a transformant having an increased frequency of homologous recombination due to suppression of a Ku gene, which is a mitosporic filamentous fungus belonging to Trichocomaceae with a vector for the deletion of a chromosomal region, which comprises a pair of homologous region arms having a nucleotide sequence that is homologous with a fragment at either end of said chromosomal region, and deleting the chromosomal region by means of homologous recombination between the resulting transformant and said vector.02-05-2009
20100062535PSOD EXPRESSION UNITS - The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.03-11-2010
20110151567Recombinant Microorganism - A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of 06-23-2011
20120202292Novel method to generate nutritional compounds from microalgae - The present invention relates to the application of genomics technology to generate nutritional compounds in algae, such as microalgae. The present invention discloses a novel method to modify algae to produce a variety of nutritional compounds. Specifically, this method utilizes microalgae genomics technology to manipulate the existing metabolic pathways in the genomes of microalgae and therefore induce the algae to modify secondary metabolic pathways to increase the level of existing nutritional compounds or produce novel nutritional compounds with a variety of commercial applications for improving human and animal nutrition and health.08-09-2012
20100297773EXPRESSION CASSETTE, USE OF THE EXPRESSION CASSETTE, VECTOR, HOST CELL, A METHOD FOR PRODUCING A POLYPEPTIDE - The subject matters of invention relate to expression cassette, use of the expression cassette, vector, host cell, a method for producing a polypeptide ensuring its stable expression by the prokaryotic host as well as an use of the expression cassette. The invention enables stable expression of the target polypeptide, in systems where DNA-dependent RNA polymerase recognises promoter regulating synthesis of target protein as well as selection marker, which is required for survival of the host.11-25-2010
20100297772METHOD FOR THE CREATION OF GENETIC DIVERSITY IN VIVO - Disclosed is a method for creating genetic diversity in vivo, comprising the following steps: fragments of at least one wild-type gene sequence are produced, each of said fragments being provided with at least one region which is homologous with a vector that is suitable for in vivo recombination; randomized bridging oligonucleotides of at least one defined oligonucleotide sequence are produced, parts of the bridging oligonucleotide being homologous with at least one fragment of the wild-type gene sequence; and the linearized vector, at least one wild-type fragment, and the randomized bridging oligonucleotide/s are introduced into an in vivo system for homologous recombination.11-25-2010
20080268543Process for Producing Cohesive Alcohol Fermentation Yeast and Cohesive Alcohol Fermentation Yeast - The invention provides a method for production of non-uracil-requiring flocculant alcohol-fermenting yeast, comprising a step of introducing the 10-30-2008
20110053274LAC EXPRESSION SYSTEM - Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.03-03-2011
20100330678PROCESS FOR THE STABLE GENE INTERRUPTION IN CLOSTRIDIA - The present invention is related to a new method for interrupting multiple DNA sequences in Clostridia, even in genes recognized to be essential for the optimal growth of Clostridii by using a counter-selectable marker that would pinpoint the cells that have lost the plasmid and acquired a modified function that permits survival without the interrupted gene. This method is easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.12-30-2010
20090203143TRANS-SPLICING MEDIATED PHOTODYNAMIC THERAPY - The present invention provides methods and compositions for conferring selective death on cells expressing a specific target precursor messenger RNA (selective target pre-mRNA). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) expressed within a cell and mediate a trans-splicing reaction resulting in the generation of a novel chimeric mRNA molecule (chimeric mRNA) capable of encoding a light producing protein or enzyme. Cell death is further mediated by the presence of a photosensitizer which upon photoactivation produces cytotoxicity.08-13-2009
20090197338Method of Increasing the Function of an AAV Vector - A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.08-06-2009
20100190259Ethanol Production - The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination. The method is particularly useful in connection with species of 07-29-2010
20110189776PROKARYOTIC RNAi-LIKE SYSTEM AND METHODS OF USE - Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5′ region and a 3′ region. The 5′ region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3′ region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.08-04-2011
20100068816Genetically engineered herbicide resistance for maintaining axenic cultures - This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells.03-18-2010
20120149116CELLULOSE DEGRADABLE YEAST AND METHOD FOR PRODUCTION THEREOF - The present invention provides a method for producing a cellulose degradable yeast, comprising the step of co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence. According to the invention, a yeast having an improved cellulose degradation ability are provided.06-14-2012
20090311791ALPHA-HEMOLYSIN DELETION MUTATION OF SES-PRODUCING STAPHYLOCOCCUS AUREUS AND CONSTRUCT THEREOF - The present invention relates to an α-hemolysin-deletion mutant of SEs-producing 12-17-2009
20080286870XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS FOR ETHANOL PRODUCTION - A strain of xylose-utilizing 11-20-2008
20090142843Glucose Transport Mutants For Production Of Biomaterial - A method is disclosed for restoring a Glu06-04-2009
20100261278METHOD CAPABLE OF INCREASING COMPETENCY OF BACTERIAL CELL TRANSFORMATION - The invention concerns bacterial strains capable of enhanced transformation efficiencies that are produced by the introduction of the F′ genetic material. The invention also concerns processes for producing transformable competent bacteria with enhanced transformation efficiencies.10-14-2010
20080213901Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells - The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.09-04-2008
20110306139Mutant Cells Suitable For Recombinant Polypeptide Production - A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of a polypeptide comprising an amino acid sequence at least 70% identical to the sequence shown in SEQ ID NO: 2, when compared with an otherwise isogenic but non-mutated cell; methods for producing said mutated cell; and methods for producing a polypeptide of interest using said mutated cell. SEQ ID NO: 2 represents a putative metalloprotease.12-15-2011
20100323448Methods For Producing Biological Substances In Enzyme-Deficient Mutants Of Aspergillus niger - The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent 12-23-2010
20120040465MODIFICATION OF THE GENOME OF A LYTIC BACTERIOPHAGE BY IMMOBILIZING SAID BACTERIOPHAGE IN THE HOST BACTERIUM THEREOF - The present invention relates to a method for the reversible immobilization of lytic bacteriophages within their modified bacterial hosts.02-16-2012
20120231546NOVEL ACETYL CoA CARBOXYLASES - Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils that are normally present in the cell or organism. Also provided herein are organisms transformed with the novel ACCases.09-13-2012
20120208279Transformation of Algal Cells - Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.08-16-2012
20090124014METHOD FOR HOMOLOGOUS RECOMBINATION IN FUNGAL CELLS - The present invention discloses a method to construct fungal cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence, comprising: providing a DNA molecule comprising a first DNA fragment comprising a desired replacement sequence flanked at its 5′ and 3′ sides by DNA sequences substantially homologous to sequences of the chromosomal DNA flanking the target sequence and a second DNA fragment comprising an expression cassette comprising a gene encoding diphtheria toxin A and regulatory sequences functional in the fungal cell operably linked thereto; transforming the fungal cells with the resulting DNA molecule; growing the cells to obtain transformed progeny cells having the DNA molecule inserted into the chromosome, wherein cells in which the DNA molecule is inserted in the chromosome via a non-homologous recombination event are selectively killed by expression of diphtheria toxin A; and obtaining cells wherein the target sequence in the chromosomal DNA sequence is replaced by the desired replacement sequence.05-14-2009
20120003742RECOMBINANT VIRUS, ESCHERICHIA COLI RETAINING THE SAME AND A PROCESS FOR PRODUCTION THEREOF - To provide a method for producing a recombinant virus retaining the characteristics of the genome of a target virus.01-05-2012
20120208280BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF - An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a 08-16-2012
20120115235ENHANCED CELLULASE EXPRESSION IN S. DEGRADANS - The invention provides organisms and methods of using and making organisms with enhanced cellulase expression.05-10-2012
20120058563PRODUCTION OF 2-KETO-L-GULONIC ACID - The present invention relates to the production of recombinant microorganisms, in particular of the genus 03-08-2012
20120156786READY-TO-USE ELECTROPORATION CUVETTE INCLUDING FROZEN ELECTROCOMPETENT CELLS - A ready-to-use electroporation cuvette is provided that includes a cuvette, first and second electrodes positioned within the cuvette and electroporation competent cells frozen in a suspension solution within the cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed. The electroporation cuvette may be sealed with a cap that may be color coded to aid the user.06-21-2012
20110091977Homologous Recombination in an Algal Nuclear Genome - Exemplary transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA of interest inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting in replacement of the target sequence of DNA with the sequence of DNA of interest. Also provided are exemplary transformation constructs, with some transformation constructs having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA of an algal cell, a second sequence of DNA similar to a corresponding second sequence of nuclear DNA of the algal cell, and a sequence of DNA of interest inserted between the first and second sequences of the transformation construct.04-21-2011
20110104806REGULATING THE PRODUCTION OF LONG CHAIN HYDROCARBONS - The invention relates to isolated polypeptides that include amino acid sequences within botryococcene synthase from different algal species. In another aspect, the invention relates to a method for increasing the production level of a botryococcene hydrocarbon molecule in a cell. The method includes increasing expression of a polynucleotide sequence that encodes botryococcene synthase in the cell. In a further aspect, the invention relates to an algal cell having a polynucleotide sequence that is genetically engineered to express a higher level of botryococcene synthase than a corresponding wild type algal cell, wherein the cell produces an increased level of a botryococcene hydrocarbon molecule than a corresponding wild type algal cell.05-05-2011
20090197339In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris - The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as 08-06-2009
20100093095COMPOSITIONS AND METHODS FOR ENHANCED BACTERIAL EXOPOLYSACCHARIDE PRODUCTION - The present invention provides nucleic acid sequences and variants thereof capable of modulating exopolysaccharide production in 04-15-2010
20100087006Use of fluorescent protein in cyanobacteria and algae for improving photosynthesis and preventing cell damage - This disclosure provides a method to reduce cell damage caused by near UV light absorption of algal or cyanobacterial cultures. The algal or cyanobacterial cells are transformed to express one or more fluorescent proteins, that absorb the harmful UV or near UV wavelengths and emits wavelengths that are photosynthetically more active. The photosynthetic pigments of the transgenic algal or cyanobacterial cell culture will then absorb the photosynthetically active light emitted by the fluorescent proteins. Accordingly the harmful effects of the UV and near UV radiation are reduced and the photosynthetic activity of the algal or cyanobacterial cells is improved.04-08-2010
20100210017COMPOSITIONS AND METHODS FOR ENHANCING TOLERANCE FOR THE PRODUCTION OF ORGANIC CHEMICALS PRODUCED BY MICROORGANISMS - Embodiments herein generally relate to methods, compositions and uses for enhancing tolerance of production of organic acids and alcohols by microorganisms. This application also relates generally to methods, compositions and uses of vectors having one or more genetic element to increase the tolerance of organic acids or alcohols by a microorganism. Certain embodiments relate to compositions and methods of enhancing the tolerance for production of 3-hydroxypropionic acid (3-HP) by bacteria. In some embodiments, compositions and methods relate to regulating the expression of an inhibitory molecule of an enhancing gene to increase production of organic acid by bacteria.08-19-2010
20120077273Methods and Compositions for Limiting Viability of a Modified Host Cell Outside of Designated Process Conditions - The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed.03-29-2012
20120252124COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT USING MULTIPLE TRANSGENES - Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter the content of alpha-tocotrienol, beta-tocotrienol, or both, in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids.10-04-2012
20110117655METHODS AND COMPOSITIONS FOR GENETICALLY MANIPULATING CLOSTRIDIA AND RELATED BACTERIA WITH HOMOLOGOUS RECOMBINATION ASSOCIATED PROTEINS - Methods for effecting homologous recombination in a bacterium of the Clostridia family are described. These methods provide enhanced capability to genetically modify clostridia.05-19-2011
20110124109DNA MOLECULES AND METHODS - The present application discloses a DNA molecule comprising a modified Group II intron which does not express the intron-encoded reverse transcriptase but which contains a modified selectable marker gene in the reverse orientation, wherein the marker gene comprises a Group I intron in forward orientation of causing expression in a bacteria cell of the class Clostridia and wherein the DNA molecule comprises sequences that allow for the insertion of the RNA transcript of the Group II intron in the chromosome of a bacterial cell of the class Clostridia. A method of introducing a nucleic acid molecule into a site of a DNA molecule in a bacterial cell of the class Clostridia is also provided. The DNA molecule and the method are useful for making mutations 05-26-2011
20080299662Method for Mrna Stabilization - A method to increase the production of a desired chemical compound in a microorganism by introduction of a DNA sequence at the 5′ end of the encoding DNA gene sequence capable of forming a stem loop and capable of increasing the stability of mRNA transcripts from one or more genes, thus stabilized mRNAs, corresponding DNA sequences and microorganisms.12-04-2008
20080299661CLONING AND/OR SEQUENCING VECTOR - A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.12-04-2008
20120322157STRESS-INDUCED LIPID TRIGGER - The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae.12-20-2012
20110045593Transgenically mitigating the establishment and spread of transgenic algae in natural ecosystems by suppressing the activity of carbonic anhydrase - Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species, as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems by suppressing the activity of the carbon concentrating mechanism.02-24-2011
20110045592METHODS OF GENOME INSTALLATION IN A RECIPIENT HOST CELL - The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material.02-24-2011
20120270323COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT - Preparation and use of isolated nucleic acids useful in altering the oil phenotype in plants. Isolated nucleic acids and their encoded polypeptides that alter alpha- and beta-tocotrienol content in seeds and oil obtained from the seeds. Expression cassettes, host cells and transformed plants containing the foregoing nucleic acids.10-25-2012
20120276638Methods of Improving the Introduction of DNA Into Bacterial Cells - The present invention relates to methods of improving the introduction of DNA into bacterial host cells.11-01-2012
20120276637GENETICALLY ENGINEERED CYANOBACTERIA - The disclosed embodiments provide cyanobacteria spp. that have been genetically engineered to have increased production of carbon-based products of interest. These genetically engineered hosts efficiently convert carbon dioxide and light into carbon-based products of interest such as long chained hydrocarbons. Several constructs containing polynucleotides encoding enzymes active in the metabolic pathways of cyanobacteria are disclosed. In many instances, the cyanobacteria strains have been further genetically modified to optimize production of the carbon-based products of interest. The optimization includes both up-regulation and down-regulation of particular genes.11-01-2012
20120276639GENETICALLY ENGINEERED RECOMBINANT ESCHERICHIA COLI PRODUCING L-TRYPTOPHAN HAVING ORIGINALLY L-PHENYLALANINE PRODUCTIVITY, AND METHOD FOR PRODUCING L-TRYPTOPHAN USING THE MICROORGANISM - The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant 11-01-2012
20120329160INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS - Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.12-27-2012
20120100616METHOD OF DOUBLE CROSSOVER HOMOLOGOUS RECOMBINATION IN CLOSTRIDIA - The invention relates to a method of double crossover homologous recombination in a host Clostridia cell comprising: a first homologous recombination event between a donor DNA molecule and DNA of the host cell to form a product of the first recombination event in the host cell, wherein the donor DNA molecule comprises a codA gene and at least two homology arms; and a second recombination event within the product of the first homologous recombination event, thereby to form a product of the second homologous recombination event in the host cell which is selectable by the loss of the codA gene; and a related vector and altered host cell.04-26-2012
20130017608METHODS OF INCREASING YIELDS OF PLEUROMUTILINS - This invention relates to a novel Pleuromutilin gene cluster and methods of increasing yields of Pleuromutilin produced by 01-17-2013
20110159595INDUCTION OF FLOCCULATION IN PHOTOSYNTHETIC ORGANSIMS - The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.06-30-2011
20110159594NUCLEIC ACIDS, BACTERIA, AND METHODS FOR DEGRADING THE PEPTIDOGLYCAN LAYER OF A CELL WALL - The invention encompasses compositions and methods for degrading the peptidoglycan layer of a cell wall. In particular, the invention encompasses compositions and methods for degrading the peptidoglycan layer of the cell wall of a gram-negative bacterium.06-30-2011
20110159593METHOD OF REDUCING MOTILITY IN BACTERIA BY OVEREXPRESSION OF A GENE OF OI-1 GENOMIC ISLAND - Z0021 was identified as a gene within the O-Island 1 (OI-1) genomic island of verocyto-toxin-producing 06-30-2011
20100035347METHOD FOR INCREASING THE SURVIVAL OF BACTERIAL STRAINS OF THE RHIZOBIUM GENUS - The present invention describes a method for increasing the survival of the bacteria of Rhizobium genus, comprising the steps of: making the bacteria to grow in a chemically defined medium; keeping the bacteria in growth stationary phase for a proper period of time; exposing the bacteria to effective quantities of indole-3-acetic acid (IAA). Within the invention scope there is an alternative method to increase the survival of the bacteria of the Rhizobium genus by means of genetic engineering comprising the steps of: making a recombinant vector codifying enzymes able to produce IAA to express in effective way in said bacteria; making the bacteria to grow in chemically defined culture medium; keeping the bacteria in growth stationary phase for a proper period of time.02-11-2010
20080227205Incorporation on Non-Naturally Encoded Amino Acids Into Proteins - The invention provides methods and compositions for in vivo incorporation of non-naturally encoded amino acids into polypeptides by 09-18-2008
20130177989Enhanced E. coli for the production of fatty acids and method of producing the same - The invention analyzed a protein sequence using the Udwary-Merski algorithm and identified a tetradomain fragment (DH1-DH2-UMA) which consists of two predicted DH-like domains and two pseudodomains N-terminal to them. This arrangement of domains and pseudodomains is fundamentally the opposite of what is typically observed in the DH cassettes of actinobacterial polyketide synthases or mammalian fatty acid synthases, both of which feature C-terminal pseudodomains. The invention modified 07-11-2013
20130115701TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS - Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.05-09-2013
20110217780TRANSGENIC ALGAE ENGINEERED FOR HIGHER PERFORMANCE - The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase09-08-2011
20110223671Methods for using positively and negatively selectable genes in a filamentous fungal cell - The present invention relates to methods for using positively and negatively selectable genes in a filamentous fungal cell to delete, disrupt, or insert a gene in a filamentous fungal cell.09-15-2011
20100317115METHODS, COMPOSITIONS AND USES FOR ENHANCING CHEMICAL TOLERANCE BY MICROORGANISMS - Embodiments herein concern compositions and methods for enhancing chemical tolerance of biomass conversion by microorganisms. In some embodiments, enhancing tolerance of biomass hydrolysate conversion includes enhancing tolerance to low molecular weight organic compounds.12-16-2010
20130157370Transformation Vector Comprising Transposon, Microorganisms Transformed with the Vector, and Method for Producing L-Lysine Using the Microorganism - The present invention relates to a transformation vector comprising the partial fragments of a gene encoding transposase, a microorganism transformed with the vector, and a method of producing lysine using the microorganism.06-20-2013
20120282701DNA PROMOTERS AND ANTRHAX VACCINES - The invention is related to intracellularly induced bacterial DNA promoters and vaccines against 11-08-2012
20120282700MODIFIED FOOD GRADE MICROORGANISM FOR TREATMENT OF INFLAMMATORY BOWEL DISEASE - The present invention relates to microorganisms that express, or have attached to their surface, a TNFα binding polypeptide. Peptides expressed or attached on the surface of microorganism are more resistant to chemical and enzymatic degradation in the gastrointestinal tract. Such microorganisms are capable of binding TNFα and therefore reducing the content of free TNFα and alleviating its pro-inflammatory effects in the gut. The invention also relates to the use of such microorganisms as medicament in the treatment of inflammatory bowel disease.11-08-2012
20130183760Vectors for directional cloning - The invention provides vectors and methods for directional cloning.07-18-2013
20130183761Methods for Incorporating Unnatural Amino Acids in Eukaryotic Cells - The invention relates to a nucleic acid comprising a nucleotide sequence encoding a tRNA orthogonal to a eukaryotic cell, said nucleotide sequence operably linked to a promoter capable of directing transcription by eukaryotic RNA polymerase III. The invention also relates to methods for incorporating unnatural amino acids in eukaryotic cells using same.07-18-2013
20130189788L-ARABINOSE FERMENTING YEAST - An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.07-25-2013
20130189787Methods, Systems And Compositions Related To Reduction Of Conversions Of Microbially Produced 3-Hydroxyproplonic Acid (3-HP) To Aldehyde Metabolites - The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of 07-25-2013
20130196441ELECTROPORATION ELECTRODE CONFIGURATION AND METHODS - Provided herein are the concept that “singularity-based configuration” electrodes design and method can produce in an ionic substance local high electric fields with low potential differences between electrodes. The singularity-based configuration described here includes: an anode electrode; a cathode electrode; and an insulator disposed between the anode electrode and the cathode electrode. The singularity-based electrode design concept refers to electrodes in which the anode and cathode are adjacent to each other, placed essentially co-planar and are separated by an insulator. The essentially co-planar anode/insulator/cathode configuration bound one surface of the volume of interest and produce desired electric fields locally, i.e., in the vicinity of the interface between the anode and cathode. In an ideal configuration, the interface dimension between the anode and the cathode tends to zero and becomes a point of singularity.08-01-2013
20120094386ENGINEERING SALT TOLERANCE IN PHOTOSYNTHETIC MICROORGANISMS - Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications.04-19-2012

Patent applications in class Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.)

Patent applications in all subclasses Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.)