Inventors list

Assignees list

Classification tree browser

Top 100 Inventors

Top 100 Assignees


PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION

Subclass of:

435 - Chemistry: molecular biology and microbiology

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435455000 Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell 390
435471000 Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.) 151
435468000 Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell 35
435441000 Mutation employing a chemical mutagenic agent 25
435449000 Fusion of cells 21
435446000 Mutation employing radiation or electricity 9
Entries
DocumentTitleDate
20110207222Methods and Systems For Manipulating Particles Using a Fluidized Bed - The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces of gravity, fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.08-25-2011
20110207221Targeted genomic modification with partially single-stranded donor molecules - Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.08-25-2011
20080268540Microinjection apparatus, trap plate and microinjection method - A microinjection apparatus has a trap plate which traps at least one cell, so as to fix a position thereof, a capillary needle which injects a substance into the cell trapped by the trap plate, a movement mechanism portion which thrusts a distal end of the capillary needle onto a in a lengthwise direction of the needle, and which withdraws the capillary needle in the lengthwise direction thereof, and a discharge control portion which discharges the substance from the distal end of the capillary needle, when the capillary needle has been withdrawn to a predetermined position by the movement mechanism portion.10-30-2008
20110195504Tubulo-Vesicular Structure Localization Signals - The invention relates to polarized cell tubulo-vesicular structure localization signals. The localization signals are utilized as research tools or are linked to polypeptides of interest or therapeutic molecules. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and/or other macromolecules to the tubulo-vesicular structures of polarized cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and/or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.08-11-2011
20130084641Compositions and Methods for Protein Production - The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.04-04-2013
20110014707Polypeptides having endoglucanase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.01-20-2011
20090253205Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from 10-08-2009
20090233364Method to Engineer MAPK Signaling Responses Using Synthetic Scaffold Interactions and Scaffold-Mediated Feedback Loops - Synthetic scaffold interactions and scaffold-mediated feedback loops are used to engineer MAPK signaling responses in cells.09-17-2009
20120115231NOVEL LYSOPHOSPHOLIPID ACYLTRANSFERASE - The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention.05-10-2012
20090011508Method for the production of a strain having a deleted region in chromosome - The purpose of the present invention is therefore to provide a transformant which will not produce a toxic substance such as Aflatoxin even after being manipulated with genetic engineering by efficiently deleting a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding the toxic substances such as Aflatoxin.01-08-2009
20090203139PRODUCTION OF FOUR CARBON ALCOHOLS USING IMPROVED STRAIN - Using screening of transposon random insertion mutants, genes involved in accumulation of (p)ppGpp were found to be involved in bacterial cell response to butanol. Reduced production of proteins with enzymatic activity for (p)ppGpp biosynthesis confers increased butanol tolerance. Bacterial strains with reduced (p)ppGpp accumulation and having a butanol or 2-butanone biosynthetic pathway are useful for production of butanol or 2-butanone.08-13-2009
20090317910MULTIPLEX AUTOMATED GENOME ENGINEERING - The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.12-24-2009
20100291681Methods and compositions for selecting siRNA of improved functionality - Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.11-18-2010
20110300631METHOD FOR SUPPRESSING CELL GROWTH - Disclosed is a method for suppressing the growth of a target cell, which is not limited in the type of a target cell and the type of a protein to be expressed in the target cell and needs not any preparatory experiment for determining a codon to be contained in a protein to be expressed in a target cell. Specifically disclosed is a method for suppressing the growth of a target cell, which comprises the steps of: incorporating DNA containing a region encoding a protein into the target cell, and allowing a protein encoded by the DNA to be expressed in the target cell into which the DNA has been incorporated. The region contained in the DNA comprises a tri-nucleotide sequence. The tri-nucleotide sequence is selected from codons that define at least some amino acid species constituting the protein, and is complementary to at least some codons that are used in the target cell at a frequency of 0.2 or less.12-08-2011
20110294217DNA NICKING ENZYME FROM A HOMING ENDONUCLEASE THAT STIMULATES SITE-SPECIFIC GENE CONVERSION - An engineered highly specific DNA-cleavage enzyme delivers a site-specific nick in a double stranded DNA, to cleave one DNA strand within its target site while leaving the opposing DNA strand intact. The engineered enzyme provides the ability to induce a gene conversion event in a mammalian cell. An engineered sequence-specific nickase derived from a LAGLIDADG homing endonuclease is altered by a single amino acid residue, wherein the amino acid residue is involved in the polarization of solvent molecules and acid-base catalysis in the active site without affecting direct contacts between the enzyme and either the bound DNA or bound metal ions. Engineered, site-specific nickase variants, such as of I-AniI and other homing endonucleases, are particularly useful in targeted genome engineering as well as therapeutic, targeted gene repair.12-01-2011
20110287545Targeted genomic modification with partially single-stranded donor molecules - Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.11-24-2011
20100009450METHOD FOR CLONING AND EXPRESSING TARGET GENE BY HOMOLOGOUS RECOMBINATION - A method for cloning and expressing a target gene by homologous recombination, and more particularly a method for cloning and expressing a target gene by homologous recombination, wherein a host cell transformed with a recombinant vector and a plasmid containing a recombinase system is introduced with a linear DNA fragment comprising a target gene and a sequence having homology to the recombinant vector. Because complicated genetic engineering steps, such as the restriction enzyme treatment and ligation of a vector and a target gene, are not required, the cloning of a gene can be performed without needing a high degree of skill, and enzyme cost can be reduced. The inventive method can be effectively used for the massive, high-speed cloning and protein expression of genes, and the disclosed pRMT-iTGR system can be used as an analytical means for improving high-efficiency recombinase.01-14-2010
20110217778Leader Sequence to Boost Gene Expression - The invention provides compositions and methods for enhanced gene expression. The invention provides a composition comprising a 28-codon leader sequence operably linked to a desired gene which encodes the desired protein.09-08-2011
20100112701REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME - The present invention relates to a protein which can asymmetrically reduce an ortho-substituted phenylglyoxalic acid compound to produce an aide or ester compound of corresponding optically-active ortho-substituted mandelic acid compound with a good optical yield, a DNA encoding the protein, a process for producing the protein from the DNA, and a process for asymmetrically reducing an ortho-substituted phenylglyoxalic acid compound to produce a corresponding optically-active ortho-substituted mandelic acid compound.05-06-2010
20090087910PRIMER-EXTENSION BASED METHOD FOR THE GENERATION OF siRNA/miRNA EXPRESSION VECTORS - Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell.04-02-2009
20100099191RNA INTERFERENCE TAGS - The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition comprising a genetic construct and the target gene being introduced into the eukaryotic cell by means of said construct, and the construct having a first target gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic cell by RNA interference. The composition comprises at least two genetic constructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene and a first siRNA tag and b) the second construct containing a second target gene and a second siRNA tag, and the first and the second siRNA tag having different nucleic acid sequences. The invention finally relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. Said method comprises the following steps: a) providing at least one eukaryotic cell, said cell being capable of RNA interference, b) transfecting the cells with a composition according to the invention and c) introducing at least one siRNA that is complementary to a siRNA tag of a transfected construct to inhibit the expression of the target gene that forms a transcription unit together with the siRNA tag.04-22-2010
20120264217RESPIRATORY SYNCYTIAL VIRUS EXPRESSION VECTORS - In certain embodiments, the disclosure relates to vectors containing bacterial nucleic acid sequences and a paramyxovirus gene. Typically, the expression vector comprises a bacterial artificial chromosome (BAC), and a nucleic acid sequence comprising a respiratory syncytial virus (RSV) gene in operable combination with a regulatory element and optionally a reporter gene.10-18-2012
20080206869Nucleic Acid Complex - The present invention relates to modification of nucleic acids for specific delivery in vitro and in vivo. More specifically, the present invention relates to modification of RNA or DNA molecules in order to add functions in terms of delivery and specificity to RNA interference or antisense technology. A specific binding domain is incorporated into the nucleic acid to which a complementary nucleic acid, conjugated to a biologically active molecule, can hybridize.08-28-2008
20080206870Pneumatic Capillary Gun for Ballistic Delivery of Microscopic Particles into Tissue - The capillary gun for delivery of ballistic particles to a target includes an inner capillary tube disposed concentrically within an outer capillary tube with the input end of the inner tube connected to a channel through which a continuous flow of high speed helium gas carrying ballistic particles is introduced. The outer capillary tube, which is connected to a vacuum source, has an outlet end that extends slightly beyond the end of the inner tube. A cap placed over the output end of the outer tube has an opening at its center through which the particles exit the device. The vacuum source applies continuous suction to the space between the outer tube and the inner tube, drawing the gas from the output end of the inner tube while the inertia of the accelerated particles causes them to continue in the axial direction through the exit opening for delivery to the target. Multiple particle injectors provide for the concurrent injection of different materials without disruption of the gas flow.08-28-2008
20090280567STABILIZED SIRNAS AS TRANSFECTION CONTROLS AND SILENCING REAGENTS - RNA molecules, including siRNA molecules and related control, trackability and exaequo agents with specific stability modifications are provided. These molecules are particularly advantageous as transfection control reagents. The molecules include first and second 5′ terminal sense nucleotides with 2′-O-alkyl groups and a label on the first 5′ terminal sense nucleotide, in conjunction with at least one additional 2′-O-alkyl pyrimidine modified sense nucleotide, and either: (i) at least one 2′ fluoro modified pyrimidine antisense nucleotide and a phosphorylated first 5′ terminal antisense nucleotide; or (ii) a first and second 5′ terminal antisense nucleotide with 2′-O-alkyl modifications and at least one additional 2′-O-alkyl pyrimidine modified antisense nucleotide.11-12-2009
20080241928METHODS AND COMPOSITIONS FOR ACID PHOSPHATASE-1 GENE INHIBITION - The ACP1 *A allele provides a means for diagnosing susceptibility of a human subject to hyperlipidemia, especially hyperlipidemia associated with metabolic syndrome, a means for treating, or preventing the onset of, hyperlipidemia and metabolic syndrome, and a means for screening and identifying drugs suitable for use in treating or preventing hyperlipidemia, especially hyperlipidemia associated with metabolic syndrome. Diagnostic kits are also provided.10-02-2008
20110269233COMPOSITIONS AND METHODS FOR REGULATING CELL OSMOLARITY - The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).11-03-2011
20090061520Synthetic Biology Vectors - The present invention provides compositions, methods and kits for generating synthetic genetic circuits in biological systems. In particular, the present invention provides vectors, reagents and methods of their use in constructing synthetic genetic circuits in bacteria.03-05-2009
20100227406METHOD FOR PURIFYING CELLS, RECOVERING CELLS, AND TRANSFECTING CELLS GENTLY - The present invention relates to a process for cell purification, for cell recovery from cultures and for the transfection of the cells under especially gentle conditions, where this process can additionally be coupled with subsequent isolation and purification of polynucleotides from the optionally transfected cells, and a kit and a device for the implementation of this process.09-09-2010
20100136692PLASMIDS AND PHAGES FOR HOMOLOGOUS RECOMBINATION AND METHODS OF USE - Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of 06-03-2010
20090068741Methods and Compositions for Improving the Health of Cells in Culture - The invention relates generally to improving the growth properties of cells in culture and more specifically to accumulating beneficial mutations in the genome of cells growing in culture. Methods are disclosed for isolating cells with improved growth properties for a number of different adverse cell culture conditions which develop during prolonged culture of cells.03-12-2009
20080213898COMPOSITIONS AND METHODS FOR NUCLEIC ACID DELIVERY - Compositions and methods are described for non-viral nucleic acid delivery. A targeting peptide capable of mediating targeting to a cell or subcellular compartment is derivatized with a photoaffinity label. Following an ionic interaction with a polynucleotide, such as DNA, and photolysis, the bioactive peptide becomes covalently attached to the DNA. Upon contact with a cell, the peptide facilities uptake of the peptide-polynucleotide conjugate into the cell or subcellular compartment. Methods for using this system for delivery of structural genes, including reporter genes, and detection of expression using bioluminescence are also described.09-04-2008
20110263024PROCESS OF CLEAN CLONING - A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid, comprising the following steps: 10-27-2011
20100075420Novel Gene Delivery Vectors for Human Mesenchymal Stem Cells - Novel gene delivery vector compositions that interact with human mesenchymal stem cells are provided, as well as methods of synthesizing and using such compositions. Such compositions may comprise a plurality of hyaluronic acid hexamers covalently attached to a branched polyethylenimine. Such methods of synthesis may comprise providing a plurality of hyaluronic acid hexamers and a branched polyethylenimine, and allowing a hexamer of hyaluronic acid to covalently attach to a branched polyethylenimine to form a conjugate. Such methods of use may comprise providing a conjugate comprising a plurality of hyaluronic acid hexamers covalently attached to a branched polyethylenimine, and administering the conjugate to a cell.03-25-2010
20120003740ELECTROPORATION APPARATUS AND METHODS - This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a gap between two electrode surfaces. Further embodiments of devices and methods described herein may eliminate the need for an end user to transfer competent cells from a capped tube to electroporation cuvette, thereby saving time and producing less waste.01-05-2012
20120258537I-CREI MEGANUCLEASE VARIANTS WITH MODIFIED SPECIFICITY, METHOD OF PREPARATION AND USES THEREOF - Method of preparing I-CreI meganuclease variants having a modified cleavage specificity, variants obtainable by said method and their applications either for cleaving new DNA target or for genetic engineering and genome engineering for non-therapeutic purposes. Nucleic acids encoding said variants, expression cassettes comprising said nucleic acids, vectors comprising said expression cassettes, cells or organisms, plants or animals except humans, transformed by said vectors.10-11-2012
20110212527METHOD TO INCREASE THE PRODUCTION OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN A ACIDITHIOBACILLUS FERROOXIDANS CULTURE BY THE INHIBITION OF ENZYMES OF TRICARBOXILIC ACID CYCLE - A method of increasing the production of extracellular polymeric substances (EPS) in an 09-01-2011
20100167404Methods of Reprogramming Animal Somatic Cells - This invention generally relates to methods to obtain mammalian cells and tissues with patterns of gene expression similar to that of a developing mammalian embryo or fetus, and the use of such cells and tissues in the treatment of human disease and age-related conditions. More particularly, the invention relates to methods for identifying, expanding in culture, and formulating mammalian pluripotent stem cells and differentiated cells that differ from cells in the adult human in their pattern of gene expression, and therefore offer unique characteristics that provide novel therapeutic strategies in the treatment of degenerative disease.07-01-2010
20130171730THERMOPHILIC AND THERMOACIDOPHILIC SUGAR TRANSPORTER GENES AND ENZYMES FROM ALICYCLOBACILLUS ACIDOCALDARIUS AND RELATED ORGANISMS, METHODS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from 07-04-2013
20130115699POLYPLEX GENE DELIVERY VECTORS - Compositions comprising linear PNAI, cyclic PNAI, linear PEI, and/or cyclic PEI, useful for delivering compounds or substances into a cell, are provided, as well as methods of making linear PNAI, cyclic PNAI, linear PEI, and cyclic PEI. Also provided are methods of using compositions comprising linear PNAI, cyclic PNAI, linear PEI, and/or cyclic PEI for introducing substances into a cell.05-09-2013
20100062532Methods And Compositions For Increasing Replication Capacity Of An Influenza Virus - In certain aspects, the present invention provides methods for increasing the replication capacity of influenza viruses in hens' eggs and/or cell culture, recombinant and/or reassortant influenza viruses with increased replication capacity, and immunogenic and vaccine compositions comprising such recombinant and/or reassortant influenza viruses. In other aspects, the invention further provides nucleic acids encoding influenza genes associated with increased replication capacity, expression vectors comprising the nucleic acids of the invention, methods for making influenza viruses with increased replication capacity, and kits useful for practice of the methods.03-11-2010
20110212526Method for producing recombinant adeno-associated virus - A method of producing recombinant adeno-associated virus (rAAV) including the following steps of cotransducing host cells with a transduction solution comprising recombinant baculovirus carrying genes of the rAAV, and culturing the cotransduced host cells in a medium.09-01-2011
20080199960Methods for the Delivery of Oligomeric Compounds - The presently disclosed subject matter relates to the delivery of oligonucleotides to cells through the delivery of a composition or reagent comprising a hybridization complex comprising a first antisense oligonucleotide which is modified to have a higher stability against degradation, and a second sense oligonucleotide which is prone to degradation. The presently disclosed subject matter furthermore relates to dendrimeric bioconjugates and compositions or reagents comprising them, wherein the bioconjugate comprises a conjugate moiety coupled to a dendrimeric structure and to their use to deliver oligomeric compounds including oligonucleotides or duplexes, as described above, to cells for modulation of gene expression (i.e. antisense or antigene therapy/research, RNA interference).08-21-2008
20110223668Method of enhancing proliferation and/or survival of mesenchymal precursor cells (MPC) - The present invention relates to methods of enhancing proliferation and/or survival of mesenchymal precursor cells (MPC) and/or progeny derived therefrom in vitro or in vivo comprising exposing the MPC or progeny to SDF-1 or analog thereof. The invention also relates to compositions comprising isolated MPCs or progeny derived therefrom and SDF-1 or analogues thereof. The present invention also relates to using such methods and compositions for ex vivo or in vivo bone formation in mammals.09-15-2011
20110236976VECTOR COMPRISING MULTIPLE HOMOLOGOUS NUCLEOTIDE SEQUENCES - The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.09-29-2011
20130149783CLEAVABLE MODIFICATIONS TO REDUCIBLE POLY (AMIDO ETHYLENIMINES)S TO ENHANCE NUCLEOTIDE DELIVERY - Polyplex formulations were prepared using p(TETA/CBA), its PEGylated analog, p(TETA/CBA)-g-PEG2k, and mixtures of the two species at 10/90 and 50/50 wt %, respectively. Increasing PEG wt % inhibited polyplex formation. This work demonstrates the feasibility of preparing homogenous polyplexes by altering the PEG wt % using a mixture of p(TETA/CBA) and p(TETA/CBA)-g-PEG2k products. Further, a single-step method of making p(TETA/CBA)-g-PEG2k is disclosed.06-13-2013
20120028356TRANSFECTION WITH MICRO EXPLOSION ENACTED BY COATED DRY ICE PARTICLES - Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.02-02-2012

Patent applications in class PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION

Patent applications in all subclasses PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATION