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Carboxylic acid ester

Subclass of:

435 - Chemistry: molecular biology and microbiology

435041000 - MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION

435132000 - Preparing oxygen-containing organic compound

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DocumentTitleDate
20130045516RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME - Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.02-21-2013
20110201067METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF - Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.08-18-2011
20100055750POLYESTER SYNTHESIS - The present disclosure provides for enzymatic polymerization to produce polyester resins suitable for use in manufacturing toners. In embodiments, crystalline copolymers, which are polyesters, may be synthesized from lactones, cyclic anhydrides, cyclic carbonates, and combinations thereof. These crystalline copolymers, in turn, may be utilized in the synthesis of toner particles.03-04-2010
20100009419MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE - A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.01-14-2010
20090111153METHOD FOR ACYLTRANSFERASE REACTION USING ACYL COENZYME A - The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester.04-30-2009
20130040350Process for the Direct Production of Fermentation Products from Biomasses in a Biofilm Reactor - A dense but oxygen permeable membrane separates the oxygen supply compartment from the fermentation compartment, which contains all microorganisms, a nutrient medium and the pretreated lignocellulose. The oxygen, necessary for the growth and the activity of the aerobic cellulolytic enzymes producing microorganisms is solely transported from the oxygen supply compartment through the membrane, which leads to an oxygen gradient within the biofilm growing on the membrane. The oxygen rich zone of the biofilm lies on the membrane whereas the biofilm further away from the membrane as well as the surrounding nutrient medium are oxygen depleted. In the aerobic biofilm the extra-cellular enzymes are produced in situ and are released into the nutrient medium where they hydrolyse the cellulose and hemicellulose into soluble monosugars, which are then converted to the desired fermentation product by suitable microorganisms in the anaerobic zones of the reactor.02-14-2013
20090155867Glycolic Acid Production by Fermentation from Renewable Resources - The present invention provides a method for the biological production of glycolic acid from a fermentable carbon source in a microorganism. In one aspect of the present invention, a process for the conversion of glucose to glycolic acid is achieved by the use of a recombinant organism comprising a host 06-18-2009
20090155866METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES - The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.06-18-2009
20090155865Esterases and their Use for Processes for Kinetic Resolution of Butinolesters - New enzymes having esterase activity and their use for processes for kinetic resolution of butinolesters.06-18-2009
20100105115ALCOHOL PRODUCTION PROCESS - The invention relates to biological processes for producing one or more desired products, including alcohols such as ethanol and butanol. The processes comprise carrying out first and second fermentations of substrates in first and second bioreactors, wherein each fermentation produces one or more desired products and/or one or more by-products that can be utilised in the other fermentation. A product and/or by-product of the first fermentation is introduced to the second bioreactor during the fermentation, and a product and/or by-product of the second fermentation is introduced to the first bioreactor during the fermentation.04-29-2010
20100105114Methods and Compositions for Regulating Sporulation - Methods and compositions are provided for producing fuel utilizing various strains of 04-29-2010
20130029388Method for Accumulation of Polyhydroxyalkanoates in Biomass with On-Line Monitoring for Feed Rate Control and Process Termination - A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.01-31-2013
20110008855Recombinant Microorganism Capable Of Producing Polylactate Or Polylactate Copolymer From Sucrose And Method For Producing Polylactate Or Polylactate Copolymer From Sucrose Using The Same - Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.01-13-2011
20130071892PROCESS FOR PRODUCTION OF POLYHYDROXYALKANOIC ACID USING GENETICALLY MODIFIED MICROORGANISM HAVING ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN - The present invention intends to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material. In accordance with the present invention, there is provided a method of producing a microorganism that produces poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material, by introducing a gene encoding R-hydratase that converts a fatty acid β-oxidation system intermediate to a monomer, (R)-3-hydroxyacyl-CoA [R-3HA-CoA], into a recombinant 03-21-2013
20130071890Production of PHA using Biogas as Feedstock and Power Source - Methods for producing bioplastics from biogas include techniques for the production of PHB using a dirty biogas (e.g., methane from landfill, digester) as both a power source for the process and as feedstock. Biogas is split into two streams, one for energy to drive the process, another as feedstock. Advantageously, the techniques may be implemented off the power grid with no dependence upon agricultural products for feedstock.03-21-2013
20130071891IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.03-21-2013
20130065286PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product.03-14-2013
20130065285Chemoautotrophic Conversion of Carbon Oxides in Industrial Waste to Biomass and Chemical Products - Systems and methods for employing chemoautotrophic micro-organisms to capture carbon from industrial waste are provided. An exemplary system comprises an industrial source, such as a cement plant, and a bioreactor including the micro-organisms. The bioreactor is fed the waste stream from the source, which provides carbon to the micro-organisms, and is also fed hydrogen, from which the micro-organisms derive their energy. Additional or alternative carbon can be provided from a gasifier fed an organic feedstock. The carbon provided to the micro-organisms is converted into chemical products which can be recovered from the bioreactor. Hydrogen can be produced by electrolysis using electricity generated by a renewable energy source.03-14-2013
20130065284MODIFIED MICROORGANISM FOR HIGHLY EFFICIENT PRODUCTION OF LACTIC ACID - A modified microorganism for high efficient production of lactic acid, an expression vector for constructing the modified microorganism, and a method of producing a lactic acid using the same are disclosed. The modified microorganism may produce lactic acid at a high level under acid conditions.03-14-2013
20130164801METHOD OF PREPARING ALKYL BUTYRATE FROM FERMENTED LIQUID USING MICROORGANISMS - Disclosed is a method of preparing alkyl butyrate, which comprises: (a) producing a fermented liquid containing butyrate salt through a fermentation process using butyric acid-producing strains; (b) obtaining an extracted liquid containing butyric acid from a continuous extracting apparatus using an extraction solvent, after converting the butyrate salt into butyric acid by adding an inorganic acid except for carbonic acid or carbon dioxide into the fermented liquid, wherein the continuous extracting apparatus includes a plurality of extraction plates which are installed inside an extraction column and perform a reciprocating motion vertically, and the broth and the extraction solvent are supplied to upper and lower portions of the column, respectively; and (c) reacting the extracted liquid and alcohol having a carbon number of 4 or less or a mixture thereof in an esterification reactor to convert a resultant product into alkyl butyrate. According to the present invention, butyric acid existing in microbic culture fluid can be efficiently extracted, and furthermore, competitively priced alkyl butyrate can be prepared without an additional process of separating the extracted solvent while minimizing energy consumption.06-27-2013
20090047717ENZYME-CATALYZED POLYCARBONATE AND POLYCARBONATE ESTER SYNTHESIS - An enzymatic process for preparing aliphatic polycarbonates via terpolymerization or transesterification using a dialkyl carbonate, an aliphatic diester, and an aliphatic diol or triol reactant. A catalyst having an enzyme capable of catalyzing an ester hydrolysis reaction in an aqueous environment is subsequently added to the reaction mixture. Next, polymerization of the reaction proceeds for an allotted time at a temperature ≦100° C. Finally, the copolymer is isolated from an the catalyst via filtration.02-19-2009
20130164800Production of Polyhydroxyalkanoates - There is provided a process for producing polyhydroxyalkanoate (PHA) comprising the steps of culturing a biomass containing PHA-producing microbes in a culture media; and hydrolyzing said PHAs-producing microbes using microorganisms selected to release PHAs from the PHAs-containing microbes.06-27-2013
20120237988BUTANOL STRAIN IMPROVEMENT WITH INTEGRATION OF A POLYNUCLEOTIDE ENCODING A POLYPEPTIDE THAT CATALYZES PYRUVATE TO ACETOLACTATE CONVERSION - The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.09-20-2012
20100203602Materials and Methods for Efficient Lactic Acid Production - The present invention provides derivatives of 08-12-2010
20100178678Method for producing cyclopent-4-ene-1,3-diol or cyclopent-4-ene-1,3-diol derivatives - The invention relates to a method for producing cis-cyclopent-4-ene-1,3-diol and cis-cyclopent-4-ene-1,3-dialkanoates by selective cis-1,2-reduction of 4-hydroxycyclopent-2-enone by means of a hydroboron, in the presence of substoichiometric quantities of a trivalent rare earth metal compound, to form cyclopent-4-ene-1,3-diol which can then be optionally reacted with an acylation agent to form cis-cyclopent-4-ene-1,3-dialkanoates, in order to simplify the reprocessing and without intermediate isolation.07-15-2010
20090123983Processes for preparing an intermediate of sitagliptin via enzymatic reduction - The invention provides enzymatic reduction processes for the preparation of 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate, particularly, (S)-methyl 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate, a key intermediate in the synthesis of Sitagliptin, and the (S)- and (R)-enantiomers of methyl 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate in high enantiomeric purity.05-14-2009
20090233338PROCESS FOR RECOVERY OF POLYHYDROXYALKANOATES FROM BIOMASS - Systems and processes for the recovery of polyhydroxyalkanoates (“PHA”) from biomass are disclosed. In certain embodiments, the process includes the steps of extracting at least a portion of PHA from a disrupted quantity of PHA-containing biomass with an acetin solvent to produce a biomass phase (including residual biomass) and a PHA phase including at least a portion of the PHA from the biomass. The biomass phase and the PHA phase are separated, and at least a portion of PHA may be isolated from the PHA phase. Systems and processes including multiple disruption and/or extraction steps also are disclosed.09-17-2009
20110300592METHOD FOR PRODUCING POLY-3-HYDROXYALKANOIC ACID - When industrially separating and purifying poly-3-hydroxyalkanoic acid produced by a microorganism, to obtain poly-3-hydroxyalkanoic acid particles having an arbitrary volume mean particle diameter with favorable productivity and with decreased amount of an organic solvent used is enabled while decreasing contaminants derived from constitutive components of cellular bodies. According to the present invention, aggregated matter of poly-3-hydroxyalkanoic acid is obtained by adjusting the amount of organic nitrogen in an aqueous suspension containing poly-3-hydroxyalkanoic acid to not greater than 1,500 ppm per weight of poly-3-hydroxyalkanoic acid; and thereafter allowing poly-3-hydroxyalkanoic acid to be aggregated in the aqueous suspension.12-08-2011
20120009636Methods and Compositions for the Recombinant Biosynthesis of Fatty Acids and Esters - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.01-12-2012
20100062503Lactic acid production from concentrated raw sugar beet juice - The present invention is in the field of the preparation of lactic acid by means of fermentation on industrial scale wherein a concentrated raw beet juice having a Brix of at least 60 is used as fermentation substrate. After dilution to the desired initial sugar concentration and addition of nutrients, the juice is fermented to lactic acid and/or lactate by means of a lactic acid-producing microorganism. Said concentrated raw beet juice is prepared by: washing and cutting sugar beet and extracting the cossettes in water, removing the beet pulp from the resulting raw beet juice, and heat treating the raw beet juice at a temperature between 50 and 90 degrees Celsius, and concentrating the raw beet juice to at least 60 Brix.03-11-2010
20110045557Novel Fusion Carbonic Anhydrase/Cellulose Binding Polypeptide Encoded by a Novel Hybrid Gene, and Method of Creating and Using the Same - The invention relates a novel hybrid carbonic anhydrase catalyst with the potential to contribute significantly to meeting targeted reductions in greenhouse gas emissions. In a preferred embodiment of the present invention at least a portion of a cellulose binding domain (CBD) of a protein is fused to another protein, carbonic anhydrase, (CA) to create a new multi-functional protein which can bind tightly to cellulose while maintaining its native catalytic ability to process CO02-24-2011
20090291480Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production - The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.11-26-2009
20090148920Integrated glyceride extraction and biodiesel production processes - Biodiesel is used to extract glycerides from biomass derived sources and the extractant containing biodiesel and glycerides is subjected to ester-forming conditions including the presence of lower alkanol to produce biodiesel, a portion of which is used for the extraction of glycerides.06-11-2009
20100081179INCREASED HETEROLOGOUS Fe-S ENZYME ACTIVITY IN YEAST - Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.04-01-2010
20120270285STEREOSPECIFIC CARBONYL REDUCTASES - Stereospecific carbonyl reductases SCR1, SCR2, and SCR3 are described herein as are nucleotide sequences that encode these reductases. These stereospecific carbonyl reductases have anti-Prelog selectivity and have specificities that are useful for fine biochemical synthesis.10-25-2012
20100099155RECOVERY OF VOLATILE PRODUCTS FROM FERMENTATION BROTH - Provided are apparatuses and processes for the removal and purification of fermentation prepared one or more volatile organic compounds. The apparatuses comprise a fermentor unit, a vacuum side stripper unit (04-22-2010
20110171702RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID - The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.07-14-2011
20100136638PRODUCTION OF 3-HYDROXYPROPIONIC ACID USING BETA-ALANINE/PYRUVATE AMINOTRANSFERASE - Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.06-03-2010
20080280336Process for the Preparation of Dihydroxy Esters and Derivatives Thereof - A process is provided for the preparation of a compound of formula (1)11-13-2008
20110207186Isoforms of Pig Liver Esterase - The invention relates to novel mutants of γPLE, to vehicles containing the same and to their use in the production of enantiomer-enriched alcohols, carboxylic acids and esters.08-25-2011
20090298144METHODS OF MANUFACTURING DERIVATIVES OF BETA-HYDROXYCARBOXYLIC ACIDS - Preparation of derivatives of β-hydroxycarboxylic acid, including β-hydroxycarboxylic acid esters, α,β-unsaturated carboxylic acid, esters of α,β-unsaturated carboxylic acid, and alkoxy derivatives.12-03-2009
20090130729Biocatalytic manufacturing of (meth) acrylic esters - The invention relates to a biocatalytic method or process for the synthesis of esters of acrylic acid and/or methacrylic acid free of positively chargeable/charged groups, comprising reacting one or more alcohols (alcohol starting materials) which are free of positively chargeable or charged groups with (meth)acrylyl-CoA, preferably in the presence of a catalyst (inorganic, organic or organometallic, or preferably a biocatalyst) capable of effecting the transfer of an alcohol radical from an alcohol starting material as defined above or below to (meth)acrylyl CoA under removal of the CoA moiety transferase activity, such as an enzyme of the transferase or hydrolase class of enzymes. The (meth)acrylyl-CoA is preferably formed by reaction of (meth)acrylic acid or its salts with coenzyme A in the presence of an energy providing substance and a biocatalyst e.g. with S-acetyl coenzyme A synthetase activity or by reaction of acrylate or methacrylate produced metabolically in the presence of a biocatalyst or metabolically.05-21-2009
20090221044Process for the Enantioselective Enzymatic Reduction of Hydroxy Keto Compounds - In a process for the enantioselective enzymatic reduction of a hydroxy ketone of general formula I09-03-2009
20110177569MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME - Provided is a mutant of propionyl-CoA transferase from 07-21-2011
20100261236Genetically Stable Plasmid Expressing PDH and FDH Enzymes - Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.10-14-2010
20100240107PROCESS FOR PRODUCING OPTICALLY ACTIVE 2-ALKYL-1,1,3-TRIALKOXYCARBONYLPROPANE - A process for producing an optically active 2-alkyl-1,1,3-trialkoxycarbonylpropane (2), comprising a step of asymmetric hydrolysis of 2-alkyl-1,1,3-trialokoxycarbonylpropane (1) by using an enzyme capable of selectively hydrolyzing an ester moiety of either one enantiomer of 2-alkyl-1,1,3-trialkoxycarbonylpropane (1), or by using a culture of a microorganism capable of producing the enzyme or a treated object thereof.09-23-2010
20130217086MODIFIED MICROORGANISM FOR PRODUCTION OF 1,4-BUTANEDIOL - A modified microorganism for production of 1,4-butanediol, an expression vector, and a method of producing 1,4-butanediol using the modified microorganism are provided. The method can be useful in producing 1,4-butanediol using a biological production process.08-22-2013
20100196973METHODS FOR THE SYNTHESIS OF FATTY DIACIDS BY THE METATHESIS OF UNSATURATED DIACIDS OBTAINED BY FERMENTATION OF NATURAL FATTY ACIDS - The invention relates to a method for the synthesis of diacids of general formula ROOC—(CH08-05-2010
20100196972PRODUCING ENERGY FROM BIOLOGICAL MATERIAL - Described are methods and systems for producing synthesis gas. In some embodiments, the method includes microbiologically converting biological material to form methane and CO2; and reacting methane and CO2 formed in (a) with water and metal to form synthesis gas. Optionally, the method also includes cutting vegetation; fermenting the vegetation to form biogas comprising methane and CO2; and reacting the biogas with water and metal to form synthesis gas and metal oxide. In some embodiments of the invention, reacted metal is regenerated from metal oxide produced in the reaction. In some embodiments the regeneration comprises reacting the oxide in a bath of boiling zinc.08-05-2010
20130130337Enzymatic Acylation Method Using an Acylphosphonate Donor - The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, uikyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR′1R″1, —NR′2R″2; R1, R2, R′1, R′2, R″1 and R″2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said function being selected from among the amine, alcohol, or thiol functions.05-23-2013
20130130338NOVEL HYDROLASE PROTEIN - It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate.05-23-2013
20110212497METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM - It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.09-01-2011
20100151541NOVEL POLYPEPTIDE HAVING ESTERASE ACTIVITY AND RECOMBINANT ESTERASE AND USE THEREOF - Polypeptide and recombinant protein having esterase activity which exhibit the amino acid sequence SEQ. ID. No. 1 and the use thereof.06-17-2010
20100124773METHODS AND PROCESSES FOR PRODUCING ESTERS - Methods are provided for producing esters. The methods comprise converting a fermentable carbon source to organic acids by fermentation with organic acid producing microorganisms, followed by catalytic esterification. The methods comprise integrated fermentation, extraction, and esterification reactions wherein the organic acids produced during fermentation are extracted into an extraction solvent and then directly reacted with an alcohol in the presence of a catalyst to form organic esters. Methods of producing esters are also provided wherein the organic acids produced during fermentation and extracted into the extraction solvent are stripped from the extraction solvent prior to being reacted with an alcohol in the presence of a catalyst to form organic esters.05-20-2010
201202445863-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.09-27-2012
20100068773MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID - The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.03-18-2010
20110081692POLYHYDROXYALKANOIC ACID COPOLYMER AND PROCESS FOR PREPARING SAME - A PHA copolymer comprising (R)-3-hydroxy-4-methyl valeric acid units and a production method thereof are provided.04-07-2011
20100311131TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE) - Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as 12-09-2010
20100003730Enzymatic Synthesis of Acetoacetate Esters and Derivatives - In one embodiment, the present invention includes a method for the synthesis of an ester derivative of acetoacetate. The method includes providing a first ester of acetoacetate and providing an alcohol. The method further includes combining the first ester of acetoacetate and the alcohol in the presence of an enzyme capable of transesterification in a non-aqueous solvent to form the ester derivative of acetoacetate. The method results in the formation of the ester derivative of acetoacetate, which, in one embodiment, is monoacetoacetin.01-07-2010
20120171738ENZYMATICALLY CATALYZED METHOD OF PREPARING MONO-ACYLATED POLYOLS - The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from 07-05-2012
20120171737PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS - Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase.07-05-2012
20110027843METHOD FOR PRODUCING POLYHYDROXYALKANOATE - A method for producing polyhydroxyalkanoate (PHA), comprising (i) culturing in a culture medium comprising terephthalic acid and/or a salt thereof and/or an ester thereof one or more bacterial strains which are capable of accumulating PHA from terephthalic acid or a salt or ester thereof and which are selected from 02-03-2011
20100035313Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same - Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA.02-11-2010
20100190221METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID - Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.07-29-2010
20100021977PROCESS FOR PREPARING ALKYL (METH)ACRYLATES USING AN ENZYMATIC CYANOHYDRIN HYDROLYSIS - The present invention relates to a process for preparing alkyl(meth)acrylates, characterized in that the process has a step in which a cyanohydrin is hydrolysed with an enzyme whose residual activity after the conversion of methacrylonitrile in the presence of 20 mM cyanide ions at 20° C. after 30 min is at least 90% of the residual activity of the enzyme which has been used under otherwise identical conditions in the absence of cyanide ions.01-28-2010
20100151542Cleaning Enzymes and Fragrance Production - The present invention provides compositions comprising an acyltransferase and an alcohol substrate for the acyl-transferase. In some particularly preferred embodiments, the composition finds use in production of a fragrant ester. In some other embodiments, the composition finds use in laundry detergents to clean stains that contain at least one triglyceride. In some further embodiments, the compositions are used to produce compounds with cleaning properties (e.g., a surfactant ester).06-17-2010
20090075350METHODS FOR PRODUCING ALKYL ESTERS - This invention relates to a method for producing an alkyl ester via a transesterification or esterification reaction. The method includes (1) mixing an oil source containing a triglyceride or a carboxylic acid and a first primary alcohol or a first secondary alcohol in a first organic solvent to form a first solution; in which each molecule of the first organic solvent contains 4-8 carbon atoms and a heteroatom; (2) reacting the triglyceride or the carboxylic acid with the first primary alcohol or the first secondary alcohol in the presence of a first lipase to produce a first alkyl ester, in which the first solution does not undergo phase separation throughout the reaction; and (3) separating the first alkyl ester from the first solution.03-19-2009
20100143987PROCESS FOR PREPARING GLYCEROL ESTERS - There is provided a process for the preparation of a compound of the formula, wherein one of R06-10-2010
20120301934Process for the Production of Esters - Methods for producing an ester from a gas comprising carbon monoxide. More specifically the invention provides for methods for producing one or more products by the anaerobic fermentation of a gaseous substrate comprising CO and the subsequent conversion of the one or more products to an ester in a down-stream reaction. The invention further provides methods for improving the efficiency of the production of esters from gaseous substrates.11-29-2012
20120301933PHA-PRODUCING BACTERIA - The present disclosure relates to isolated bacterial strains producing polyhydroxyalkanoate (PHA), microbial consortia including such strains, and cultures of such strains and microbial consortia. In particular, the present disclosure relates to compositions including such strains, microbial consortia, and cultures and methods of use thereof.11-29-2012
20120149076INTEGRATION OF FERMENTAITON WITH MEMBRANE - Herein disclosed is a method comprising a) fermenting biomass to produce a fermentation broth; b) separating the fermentation broth into a liquid stream and a solid or slurry stream; c) passing the liquid stream through a reverse osmosis membrane to obtain a permeate and a retentate; and d) concentrating the retentate. Herein disclosed is a method comprising a) fermenting biomass in a first fermentor to produce a first fermentation broth; b) separating the first fermentation broth into a first liquid stream and a first solid or slurry stream; c) introducing the first solid or slurry stream into a second fermentor to produce a second fermentation broth, wherein the second fermentor comprises a lower fermentation products concentration than the first fermentor; d) separating the second fermentation broth into a second liquid stream and a second solid or slurry stream; and e) passing the second liquid stream through a reverse osmosis membrane.06-14-2012
20100196974Chemically Inducible Expression of Biosynthetic Pathways - Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.08-05-2010
20090186391Method for Production of Erythro-or threo-2-Amino-3-Hydroxypropionic Acid Ester, Novel Carbonyl Reductase, Gene for the Reductase, Vector, Transformant, and Method for Production of Optically Active Alcohol Using Those - The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus 07-23-2009
20090317879Use of selection pressures to enable microbial biosynthesis of polyhydroxyalkanoates from anaerobic degradation products - A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.12-24-2009
20120045802Liquid fractionation method for producing biofuels - A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.02-23-2012
20120045801RECOMBINANT RALSTONIA EUTROPHA CAPABLE OF PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COMPOLYMER, AND METHOD FOR PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COPOLYMER USING SAME - Provided are a recombinant 02-23-2012
20110165640METHOD FOR THE ENZYMATIC PRODUCTION OF 2-HYDROXY-2-METHYL CARBOXYLIC ACIDS - Provided is a composition containing: an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and/or a heterodimeric enzyme containing: the isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 2. Also provided is a process for producing a 2-hydroxy-2-methyl carboxylic acid or a salt or ester thereof, wherein the process involves: contacting a 3-hydroxy carboxylic acid or a salt or ester thereof with the above-mentioned composition to produce the 2-hydroxy-2-methyl carboxylic acid or the salt or ester thereof.07-07-2011
20120156738PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION - An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.06-21-2012
20110104768METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM - The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.05-05-2011
20110104767METHOD FOR PRODUCING POLYHYDROXYALKANOATE (PHAs) USING HALOBACTERIUM AND HALOBACTERIUM - Disclosed are a method for producing polyhydroxyalkanoates (PHAs) using a halobacterium belonging to the genus 05-05-2011
20120122165RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME - This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the 05-17-2012
20100248318HYDROGEN PROCESSING AND IMPURITY REMOVAL AND CLEANING METHODS IN A BIOMASS CONVERSION PROCESS - In one embodiment, the disclosure includes a method of biomass conversion including fermenting biomass to produce a carboxylic acid or carboxylate salt and hydrogen gas, recovering the hydrogen gas, and converting the carboxylic acid or carboxylate salt to an alcohol using the hydrogen gas. In one embodiment, the hydrogen produced by biomass conversion may be converted to an acetate. Another embodiment relates to a biomass conversion system. The system may include: a fermentation unit for fermentation of biomass to a carboxylic acid or carboxylate salt in a fermentation broth and for production of a carbon dioxide and hydrogen gas stream, an extraction unit for extracting the carboxylic acid or carboxylate salt from the fermentation broth, a gas extraction unit for separation of the hydrogen gas and the carbon dioxide, and a production unit for production of an alcohol from the carboxylic acid or carboxylate salt using the hydrogen gas.09-30-2010
20110183388Extracellular Polyhydroxyalkanoates Produced By Genetically Engineered Microorganisms - The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).07-28-2011
20100209984PROCESS FOR THE PREPARATION OF DIHYDROXY ESTERS AND DERIVATIVES THEREOF - A process is provided for the preparation of a compound of formula (1)08-19-2010
20100209983PREPARING METHOD FOR (S)-3HYDROXYBUTYRIC ACID AND (S)-3 HYDROXYBUTYRATE ESTER USING RECOMBINANT MICROORGANISM - A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding b ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding b ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided.08-19-2010
20120214213RECOMBINANT HOSTS AND METHODS FOR MANUFACTURING POLYHYDROXYALKANOATE - Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (P08-23-2012
20120135480METHODS FOR PRODUCING POLYHYDROXYALKANOATES FROM BIODIESEL-GLYCEROL - Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA; e.g., crystallization temperature, to enhance performance of the PHA during injection molding.05-31-2012
20100173370Fuel Production - An apparatus that includes a first reactor and a return mechanism. The first reactor has an inlet to receive a mixture comprising a first reactant, a second reactant, a reaction product, and an inert solvent that dissolves at least a portion of the first and second reactants, an enzyme to facilitate a reaction between the first and second reactants to generate more reaction product, and an outlet to output the reaction product, including the reaction product received at the inlet and the reaction product generated from the reaction between the first and second reactants. The return mechanism sends at least a portion of the reaction product from the outlet back to the inlet.07-08-2010
20120252081USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION - The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients.10-04-2012
20090035831Enantioselective immobilized lipase - Immobilization of 02-05-2009
20100273223New Calb Muteins and Their Use - A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.10-28-2010
20100291644PROCESS FOR PREPARING METHACRYLIC ACID OR METHACRYLIC ESTERS - The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of 11-18-2010
20120083021Low odor coating compositions and paints - This invention provides low odor stable coating compositions, and methods of their production. The stable aqueous coating compositions and paints comprise one or more emulsion-polymerized addition polymer and one or more carboxylesterase enzyme having an ester hydrolysis activity in the composition of less than 0.03 micromole/minute, wherein the aqueous coating composition has a headspace volatile organic compound (VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., of less than 10 ppm of the one or more organic carboxylester with a normal boiling point of less than 150° C. and more than 50 ppm of the one or more mono-alcohol with a formula molecular weight of less than 76.04-05-2012
20120264182USE OF A PROTEIN HOMOLOGOUS TO A MEAB PROTEIN FOR INCREASING THE ENZYMATIC ACTIVITY OF A 3-HYDROXYCARBOXYLIC ACID-COA MUTASE - The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.10-18-2012
20120264181METHOD FOR PRODUCING BUTYRIC ACID, BUTANOL AND BUTYRATE ESTER - The present disclosure is directed to methods for producing butyric acid comprising fermenting a feedstock using a bacterium. The feedstock comprises lactic acid, or the feedstock comprises lactic acid and at least one carbohydrate.10-18-2012
20120258507DETERGENT COMPOSITIONS CONTAINING THERMOBIFIDA FUSCA LIPASE AND METHODS OF USE THEREOF - The present compositions and methods relate to a lipase cloned from 10-11-2012
20110124064ESTERIFICATION PROCESS OF PROSTAGLANDINS AND ANALOGUES THEREOF - The invention relates to a process for enzymatically catalyzed esterification of prostaglandins or analogues thereof.05-26-2011
20130171704DICARBOXYLIC ACID PRODUCTION PROCESS - Process for producing a dicarboxylic acid comprising fermenting a microorganism in a suitable fermentation medium wherein a gas flow comprising 30% to 100% v/v oxygen as measured under atmospheric pressure is added to the fermentation medium and producing the dicarboxylic acid.07-04-2013
20080299627Method For Production Of High Purity Polyhydroxyalkanonate (PHAs) - The present invention relates to a method of producing polyhydroxyalkanoates (PHAs) using 12-04-2008
20110039315Methods and Systems for Biomass Conversion to Carboxylic Acids and Alcohols - The disclosure includes a method, process and apparatus for the conversion of biomass to carboxylic acids and/or primary alcohols. The system may include a pretreatment/fermentation subsystem operable to produce a fermentation broth containing carboxylic acid salts from biomass, such as lignocellulosic biomass. The system may also include a dewatering subsystem operable to remove excess water from the fermentation broth to produce a concentrated product. The system may also includes an acid springing subsystem operable to produce a mixed carboxylic acid product. The system may also include a hydrogenation subsystem operable to produce an alcohol mixture, such as a mixture containing primary alcohols. Methods of operating this system or other systems to obtain a carboxylic acid or alcohol mixture are also provided.02-17-2011
20110236936METHOD FOR ENHANCING PRODUCTION POLYHYDROXYALKANOIC ACID FROM MICROORGANISM STRAINS - Provided is a method for enhancing the production of polyhydroxyalkanoic acid (PHA) from microorganism strains by disrupting a gene associated with the production of an exobiopolymer (EBP) in the 09-29-2011
20100233768METHOD FOR ISOLATING POLYHYROXYALKANOATES - The invention relates to a method for isolating polyhydroxyalkanoates from production cells which comprises09-16-2010
20100136637RECOMBINANT MICROORGANISM HAVING A PRODUCING ABILITY OF POLYLACTATE OR ITS COPOLYMERS AND METHOD FOR PREPARING POLYACTATE OR ITS COPOLYMERS USING THE SAME - Provided are a recombinant microorganism capable of producing polylactate (PLA) or hydroxyalkanoate-lactate copolymers and a method of preparing PLA or hydroxyalkanoate-lactate copolymers using the same. The recombinant microorganism has both a gene encoding a propionyl-CoA transferase from 06-03-2010
20120100582PIG LIVER ESTERASES - The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in 04-26-2012
20120288907Glucose Transport Mutants for Production of Biomaterial - A method is disclosed for restoring a Glu11-15-2012
20130017583METHODS FOR PRODUCING POLYHYDROXYALKANOATE COPOLYMER WITH HIGH MEDIUM CHAIN LENGTH MONOMER CONTENT - The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression.01-17-2013
20110159556Use of hydroxyalkanoic acids as substrates for production of poly-hydroxyalkanoates by methane-oxidizing bacteria - A method of biosynthesis of polyhydroxyalkanoates (PHA) is provided that includes providing a type II methanotrophic bacteria, and disposing the type II methanotrophic bacteria in an unbalanced growth condition, where the unbalanced growth condition includes a nutrient-deficient media and a hydroxyalkanoic acid, and where the nutrient-deficient media has an absence of an essential nutrient required for cell replication of the type II methanotrophic bacteria.06-30-2011
20110159555Methods, Chimeric Polypeptides, Polynucleotides and Cells for Producing Bioplastics - Methods of producing a bioplastic are disclosed. Furthermore, chimeric polypeptides useful to transport polyhydroxyalkanoates (PHAs) are disclosed, as are polynucleotides that code for the chimeric polypeptides, cells harboring the chimeric polypeptides and methods of transporting PHAs.06-30-2011
20080248539COMPOSITIONS AND METHODS FOR PRODUCING STEREOISOMERICALLY PURE STATINS AND SYNTHETIC INTERMEDIATES THEREFOR - The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.10-09-2008
20080233620Novel Transformant and Process for Producing Polyester Using the Same - The present invention provides a process for producing yeast excellent in cell productivity and gene manipulation of which is easy, being added with nutritional requirement by disrupting only a specific gene, and a transformant thereof. Moreover, the present invention also provides a process for producing a gene expression product, particularly a polyhydroxyalkanoic acid.09-25-2008
20130177952METHOD OF PRODUCING INGENOL-3-ANGELATE - The present invention relates to methods of producing ingenol-3-angelate (I) from ingenol (II).07-11-2013
20130171703PRODUCING ENERGY FROM BIOLOGICAL MATERIAL - Described are methods and systems for producing synthesis gas. In some embodiments, the method includes microbiologically converting biological material to form methane and CO2; and reacting methane and CO2 formed in (a) with water and metal to form synthesis gas. Optionally, the method also includes cutting vegetation; fermenting the vegetation to form biogas comprising methane and CO2; and reacting the biogas with water and metal to form synthesis gas and metal oxide. In some embodiments of the invention, reacted metal is regenerated from metal oxide produced in the reaction. In some embodiments the regeneration comprises reacting the oxide in a bath of boiling zinc.07-04-2013
20130122559METHOD FOR SYNTHESIZING POLYHYDROXYALKANOATE USING A MICROORGANISM - A method for synthesizing polyhydroxyalkanoate using a microorganism includes providing a 05-16-2013
20080199925Method For Producing an Aqueous Polymer Dispersion - A process for preparing an aqueous polymer dispersion, in which, in an aqueous medium, in a first reaction stage, a hydroxycarboxylic acid compound is reacted in the presence of an enzyme and of a dispersant, and also, if appropriate, of a low water solubility organic solvent and/or of an ethylenically unsaturated monomer, to give a polyester, and thereafter, in the presence of the polyester, in a second reaction stage, an ethylenically unsaturated monomer is free-radically polymerized.08-21-2008
20080199924Production of Degummed Fatty Acid Alkyl Esters - The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.08-21-2008
20130203134METHOD FOR PRODUCING L-LACTIC ACID BY LACTIC ACID BACTERIUM UNDER PRESENCE OF PENTOSE AND CELLOOLIGOSACCHARIDES - The present invention thus provides a method for producing L-lactic acid, which comprises the step of culturing a lactic acid bacterium that can produce L-lactic acid in a medium containing any one selected from the group consisting of cellobiose, cellooligosaccharides, xylose, arabinose, and glucose derived from cellulose and/or hemicellulose as a substrate to obtain L-lactic acid. In a preferred embodiment of the present invention, 08-08-2013
20130149755USE OF OXYHYDROGEN MICROORGANISMS FOR NON-PHOTOSYNTHETIC CARBON CAPTURE AND CONVERSION OF INORGANIC AND/OR C1 CARBON SOURCES INTO USEFUL ORGANIC COMPOUNDS - Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including bio-fuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO06-13-2013
20110275127Process for Producing Ethanol - A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid into ethanol using esterification and hydrogenation reactions. Coproducts can include corn oil, and high protein animal feed containing the biomass produced in the fermentation.11-10-2011
20120258506CELL-BASED SYSTEMS FOR PRODUCTION OF METHYL FORMATE - Disclosed is a process in which a recombinant organism, such as a yeast, expressing a heterologous S-adenosylmethionme (SAM)-dependent methyl halide transferase (MHT) protein is combined with a halide and a carbon source in a cultivation medium under conditions in which methyl formate is produced. The cell may genetically modified to express methyl formate synthase, methanol dehydrogenase and/or hydrolytic dehalogenase at levels higher than a cell of the same species that is not genetically modified. The methyl formate may be collected and used in a variety of applications. The halide may be chlorine, bromine or iodine.10-11-2012
20130157326INTEGRATED BIOPROCESSING FOR FUEL PRODUCTION - Systems and methods are provided for enhancing the integration of processes for recovering products from algae-derived biomass. The enhanced process integration allows for increased use of input streams and other reagents that are derived from renewable sources. This increases the overall renewable character of the products extracted from the algae-derived biomass. The process integration can include exchange of input streams or energy between an algae processing system and a system for processing non-algal biomass. One example of improving process integration is using oxygenates that are generated in a renewable manner as a reagent for enhancing the algae processing system.06-20-2013
20130157327MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE GENE AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME - A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from 06-20-2013
20130157328CATALYTIC DEHYDRATION OF LACTIC ACID AND LACTIC ACID ESTERS - This invention relates to catalytic dehydration of lactic acid derived from biological fermentation and its esters into acrylic acid and acrylic acid esters respectively. Disclosed in this invention are chemical catalysts suitable for industrial scale production of acrylic acid and acrylic acid esters. This invention also provides an industrial scale integrated process technology for producing acrylic acid and acrylic acid esters from biological fermentation using renewable resources and biological catalysts.06-20-2013
20110287498PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product.11-24-2011
20110294176METHOD OF EXTRACTING BUTYRIC ACID FROM A FERMENTED LIQUID AND CHEMICALLY CONVERTING BUTYRIC ACID INTO BIOFUEL - Disclosed is a method of converting butyric acid contained in a fermentation broth into biofuel. This chemical conversion method includes separating biohydrogen from gases generated in the course of production of butyric acid through fermentation of carbohydrate, extracting butyric acid from the broth using an insoluble solvent, esterifying butyric acid thus producing butylbutyrate, and hydrogenolyzing all or part of butylbutyrate, thus obtaining butanol. Thereby, biobutanol can be efficiently and economically produced, and butylbutyrate, which has oxidation stability superior to that of conventional biodiesel (fatty acid methyl ester) and is thus regarded as novel biofuel, can be produced together.12-01-2011
20120021471BIOABSORBABLE POLYMER CONTAINING 2-HYDROXYACID MONOMERS - Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer.01-26-2012
20120028320PREPARATION OF ADIPIC ACID - The invention relates to a method for preparing adipic acid, comprising converting alpha-ketoglutaric acid (AKG) into alpha-ketoadipic acid (AKA), converting alpha-ketoadipic acid into alpha-ketopimelic acid (AKP), converting alpha-ketopimelic acid into 5-formylpentanoic acid (5-FVA), and converting 5-formylpentanoic acid into adipic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst.The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in said method.02-02-2012
20130196395ENZYME-CONTAINING MINI-EMULSIONS - The present invention relates to a mini-emulsion which comprises at least one hydrolase, where the continuous phase of the mini-emulsion contains at least one oxidant, while the dispersed phase comprises at least one C08-01-2013
201200772363-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.03-29-2012
20120088280GENE ENCODING POLYMER SYNTHASE AND A PROCESS FOR PRODUCING POLYMER - An isolated polynucleotide encoding for a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 with polymer synthase activity.04-12-2012
20130210096Methods and Systems for the Production of Hydrocarbon Products - Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.08-15-2013

Patent applications in class Carboxylic acid ester