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Fat; fatty oil; ester-type wax; higher fatty acid (i.e., having at least seven carbon atoms in an unbroken chain bound to a carboxyl group); oxidized oil or fat

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435 - Chemistry: molecular biology and microbiology

435041000 - MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION

435132000 - Preparing oxygen-containing organic compound

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DocumentTitleDate
20090325247METHODS FOR PRODUCING CERAMIDE USING TRANSFORMED YEAST - The present invention provides methods for producing human ceramide in a yeast cell.12-31-2009
20090298141Continuous Chemical Proceses in Centrifugal Contact Separators - The invention relates to the use of a centrifugal contact-separator for carrying out a non-radioactive reaction in a liquid-liquid emulsion formed from two immiscible liquids. The invention also relates to a process for carrying out a reaction in a centrifugal contact-separator, and to a process for carrying out a catalytic reaction in a centrifugal contact-separator. An example of a process for carrying out a reaction, comprising the following steps: i) continuously introducing a liquid phase A and a liquid phase B into at least one first centrifugal contact separator, where in liquid phases A and B are immiscible and wherein phase A and/or phase B comprise at least one reactant ii) mixing phase A and phase B thereby allowing an emulsion to be formed iii) applying a centrifugal force that allows phase separation of the emulsion, such that phases A′ and B′ are obtained; iv) optionally, recovering a reaction product from at least one or the phases A′ and B′.12-03-2009
20120184004METHODS AND COMPOSITIONS FOR THE PRODUCTION OF FATTY ACIDS IN PHOTOSYNTHETIC PROKARYOTIC MICROORGANISMS - Provided herein are methods and compositions for the production of renewable fatty acids from photosynthetic prokaryotic microorganisms, such as a blue green algae, any specie of the phylum Cyanophyta, a chloroplast of a green algae and/or a Cyanobacterium. Engineered or natural strains of these organisms or organelles can be used to produce both saturated and unsaturated fatty acids with variable chain length specificity from 8-18 carbons. The fatty acids can then be secreted into the culture medium, allowing for rapid, continuous and efficient separation of fatty acid product without harvesting of cell mass.07-19-2012
20120184003PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS EXPRESSING POLYPEPTIDES HAVING LIPOLYTIC ACTIVITY - The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.07-19-2012
20120184002CULTURING A MICROORGANISM IN A MEDIUM WITH AN ELEVATED LEVEL OF A CARBOXYLATE COUNTERION SOURCE - The invention provides a method of culturing a microorganism that produces a free fatty acid in a culture medium, comprising culturing the microorganism in the culture medium comprising a sufficient amount (e.g., at least about 0.5 mM) of a carboxylate counterion source to enhance viability of the microorganism in the culture medium. Fatty acids produced using the methods of the invention can be used to synthesize a variety of products, including biofuels.07-19-2012
20120184001SYSTEMS AND METHODS FOR SUSTAINABLE AQUACULTURE - Provided herein are systems and methods for sustainable aquaculture. The methods provided herein allows recovery and/or recycling of autochthonous nutrients in fish farming, and recovery of allochthonous nutrients present in eutrophic water. The systems provided herein comprises two closely spaced cages, an array of rotary panels that regulates the flow of matters between the two cages; and a means for producing a directional water current.07-19-2012
20090104674Diacylglycerol Acyltransferases for Alteration of Polyunsaturated Fatty Acids and Oil Content in Oleaginous Organisms - Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., 04-23-2009
20120178135ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYENOIC FATTY ACIDS BY VERY HIGH DENSITY CULTURES OF EUKARYOTIC MICROBES IN FERMENTORS - The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.07-12-2012
20130078685OZONOLYSIS OF UNSATURATED FATTY ACIDS AND DERIVATIVES THEREOF - The invention relates to a method for the ozonolysis of unsaturated fatty acids and derivatives thereof, comprising a) ozonolysis of a fatty acid or of a derivative thereof in an organic phase comprising an organic solvent, and b) contacting the organic phase with an aqueous phase comprising catalase and preferably a buffer, where the fatty acid or the derivative thereof has a linear chain having at least eight carbon atoms, and the use of catalase for removing reactive oxygen species from a reaction mixture comprising ozonolysis products of an unsaturated fatty acid.03-28-2013
20130078686PRODUCTION AND SECRETION OF FATTY ACIDS AND FATTY ACID DERIVATIVES - The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms.03-28-2013
20090203094Use Of Genes For Increasing The Oil Content In Plants - The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing the Ypr140w polypeptide from yeast or corresponding polypeptides from plants. The invention furthermore relates to expression constructs for expressing the yeast polypeptide Ypr140w or corresponding polypeptides from plants in plants, preferably in plant seeds, the transgenic plants expressing the polypeptide and to the use of the transgenic plants for the production of food, feed, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.08-13-2009
20100075386MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b03-25-2010
20100075387DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.03-25-2010
20120244585METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS FROM ALGAL OIL - A method for producing biofuels is provided that includes dewatering intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrodeoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock. The method further includes supplying the hydrogenation and deoxygenation processes with hydrogen produced from reformed light hydrocarbons or an algae culture.09-27-2012
20130078684ACYL-ACP WAX ESTER SYNTHASES - The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.03-28-2013
20130084611Novel Plant Acyltransferases Specific for Long-Chained, Multiply Unsaturated Fatty Acids - The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.04-04-2013
20130137149PRODUCTION OF BIODIESEL BY YEAST FROM LIGNOCELLULOSE AND GLYCEROL - The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to over-produced and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method.05-30-2013
20130034886PSEUDOMONAS AERUGINOSA STRAIN DEVELOPED FOR IMPROVING FATTY ACID CONTENT, AND METHOD OF MANUFACTURING THE SAME - Disclosed are novel 02-07-2013
20130034887Tailored Oils Produced from Recombinant Oleaginous Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.02-07-2013
20130040349Method for capture carbon & storage (CCS process) from coal fuel gas and the storage as biofuels: oil, gasoline, biodiesel, jet fuel, ethanol, and methane - A Capture Carbon Storage (CCS) Process for the economical capture of carbon dioxide from coal fuel gas, and the storage of the carbon dioxide as lipid oil or the use of the environmentally begin oil for transportation. The lipid oil may be refined into gasoline, biodiesel fuel, jet fuel, ethanol, and methane which provide a renewable energy resource for the United State's future energy needs. It is a new fuel form which has been both chemically and physically altered to reduce the emissions of carbon dioxide. It is a coal cleaning/upgrading process which produces a refined fuel, Hydrogen, that may be used to produce electricity and an environmentally begin biofuel for transportation. The renewable fuel will produce no carbon foot print at the internal combustion engine's exhaust pipe. The CCS Process will produce a 100% reduction in carbon dioxide (CO02-14-2013
20100323415Emulsions in Enzymatic Reactions - A reaction medium for enzyme-catalyzed reactions is provided, comprising an oil-in-water emulsion including water, an emulsifier, an oil phase and at least one interfacially active enzyme, where the emulsion is produced by the phase inversion temperature process and has a droplet size of 50 to 400 nm. A method for the enzyme-catalyzed esterification, transesterification or hydrolysis of fatty acid alkyl esters and/or triglycerides is also provided where the oil-in-water emulsion is used as the reaction medium.12-23-2010
20100041114PROCESS FOR PRODUCING USEFUL SUBSTANCE WITH IMMOBILIZED ENZYME - The present invention relates to a process for producing a useful substance, which includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and performing a reaction while allowing the liquid mixture to flow in the same direction in a co-current manner, wherein the fixed bed-type reaction column has partition plates inserted in the longitudinal direction of the fixed bed-type reaction column so as to form a plurality of tube-shaped structures, wherein each tube-shaped structure has a lateral cross-section which is circular or polygonal in shape with at least a part being unclosed and has a representative length of 02-18-2010
20100041113DELTA 6 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ6 desaturases, which have the ability to convert linoleic acid [“LA”; 18:2 ω-6] to γ-linolenic acid [“GLA”; 18:3 ω-6] and/or α-linolenic acid [“ALA”; 18:3 ω-3] to stearidonic acid [“STA”; 18:4 ω-3]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ6 desaturases, along with methods of making long-chain polyunsaturated fatty acids [“PUFAs”] using these Δ6 desaturases in oleaginous yeast, are disclosed.02-18-2010
20100041112Production of Biodiesel - The invention relates to the production of biodiesel feedstock and biodiesel. A process for producing a biodiesel utilising a thraustochytrid is provided.02-18-2010
20100041111Immobilized Enzymes and Methods of Using Thereof - The disclosed matter relates to immobilized enzymes and methods of use thereof.02-18-2010
20130029387MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS - A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from 01-31-2013
20100093046ENERGY PRODUCTION WITH HYPERTHERMOPHILIC ORGANISMS - The present invention relates to the field of degradation with hyperthermophilic organisms, and in particular to the use of hyperthermophilic degradation to produce heat and energy rich components including hydrogen and ethanol from a biomass. In some embodiments, a biomass is fermented in the presence of hyperthermophilic organisms to produce heat. The heat is used to heat a liquid which is used directly in a heat pump or radiant heat or to produce electricity or drive a steam turbine. In some embodiments, acetate is utilized as a substrate to produce energy by methanogenesis.04-15-2010
20120164701RENEWABLE CHEMICALS AND FUELS FROM OLEAGINOUS YEAST - The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.06-28-2012
20120164700PROKARYOTIC ACYL-ACP THIOESTERASES FOR PRODUCING FATTY ACIDS IN GENETICALLY ENGINEERED MICROORGANISMS - The described invention provides genetically engineered photosynthetic microorganisms expressing prokaryotic acyl-ACP thioesterases and methods of using the genetically engineered photosynthetic microorganisms for producing free fatty acids and/or fatty acid derivatives.06-28-2012
20090123982Triglyceride Process - A process for producing triglycerides which comprises: (a) subjecting a first triglyceride comprising at least 40% by moles of oleic acid residues, based on total acyl groups in the triglyceride, to an alcoholysis reaction with an alcohol having from 1 to 6 carbon atoms to obtain a composition comprising 2-oleoyl monoglyceride and at least one acyl ester of said alcohol; (b) reacting the 2-oleoyl monoglyceride with an acylating agent comprising at least one C12 to C24 saturated fatty acid, at least one ester of said fatty acid or a mixture thereof, to obtain a 1,3-saturated fatty acid acyl 2-oleoyl glyceride; and (c) separating at least a part of the at least one acyl ester after or during step (a) or step (b); (d) reacting the acyl ester with a second glyceride to form 1,3-dioleoyl 2-palmitoyl glyceride.05-14-2009
20130052702Methods for Enhancing By-Products From Fermentation Processes - The present invention relates to methods for enhancing yield and/or quality of by-products from fermentation processes used for producing fermentation products. The invention includes a process of fermenting starch-containing material into a fermentation product comprising a fermentation step wherein one or more enzymes are added before or during the fermentation step. More specifically, the present invention provides methods for increasing oil recovery yield from processes for producing fermentation products and further enhancing the quality of DDGS by removing fat content from DDGS that are produced as by-products in processes for producing fermentation products.02-28-2013
20130052701ENZYMATIC TRANSESTERIFICATION/ESTERIFICATION PROCESSES EMPLOYING LIPASES IMMOBILIZED ON HYDROPHOBIC RESINS IN THE PRESENCE OF WATER SOLUTIONS - Disclosed are an enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries and a system therefor. The process utilizes enzymes immobilized on a hydrophobic resin mixed with a fatty acid source and an alcohol or alcohol donor in the presence of an alkaline or mild alkaline aqueous buffer, or in the presence of water or aqueous solution. The production process for fatty acid alkyl esters is carried out by transesterification or esterification simultaneously or sequentially. The biocatalyst activity is maintained with no significant activity losses in multiple uses and also avoids the accumulation of glycerol and water by-products or other hydrophilic compounds on the biocatalyst.02-28-2013
20130089901NOVEL THRAUSTOCHYTRID-BASED MICROALGAE, AND METHOD FOR PREPARING BIO-OIL BY USING SAME - The present invention relates to novel thraustochytrid-like microalgae having the ability to produce bio-oil, and a method of producing bio-oil using the same. The microalgae of the invention accumulate bio-oil at a high ratio in the cells when being cultured in glucose-containing medium, and thus can produce bio-oil in a high yield. Also, the microalgae can produce bio-oil using bean powder as a nitrogen source, and a product obtained by culturing edible bean powder as medium can be used as a raw material for producing food and feed. Also, the microalgae can produce bio-oil using non-food cellulosic biomass as a carbon source. Moreover, the use of non-food cellulosic biomass for production of bio-oil can overcome the factors limiting the development of bio-oil, including the unstable supply of food resources and an increase in the cost of raw materials, and can improve the commercial competitiveness of microbial fermentation oil.04-11-2013
20130089900Extraction Solvents Derived from Oil for Alcohol Removal in Extractive Fermentation - In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.04-11-2013
20130089899INDUSTRIAL FATTY ACID ENGINEERING GENERAL SYSTEM FOR MODIFYING FATTY ACIDS - Compositions and methods for a hybrid biological and chemical process utilizing chemotrophic microorganisms that converts syngas and/or gaseous CO2 and/or a mixture of CO2 gas and H2 gas into one or more desaturated hydrocarbons, unsaturated fatty acids, hydroxy acids, or diacids.04-11-2013
20090305365FAD4, FAD5, FAD5-2, AND FAD6, NOVEL FATTY ACID DESATURASE FAMILY MEMBERS AND USES THEREOF - The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.12-10-2009
20130071889PRODUCTION METHOD FOR BIOFUEL - The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S03-21-2013
20130164795CO-PRODUCTS FROM BIOFUEL PRODUCTION PROCESSES AND METHODS OF MAKING - The present invention includes methods of generating co-products for animal feed and compositions useful as co-products for animal feed derived from biofuel production processes. More specifically, the invention includes co-products for animal feed from at least one process feedstream, such as fatty acids from oil hydrolysis, lipids from evaporation of thin stillage, syrup, distillers grains, distillers grains and solubles, solids from a mash before fermentation, and solids from a whole stillage after fermentation.06-27-2013
20130164796SYSTEMS AND METHODS FOR CONTAMINANT REMOVAL FROM A MICROALGAE CULTURE - Systems and methods for the removal of contaminants from a liquid culture microalgae and/or cyanobacteria comprise an let tube, a pump, a gas injector, a vertical chamber, and/or a collection container that promotes the production of foam in the microalgae culture, wherein the foam contains the contaminants.06-27-2013
20130164797PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM - A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.06-27-2013
20130164798PROCESSES FOR PRODUCING HYDROCARBON PRODUCTS - The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.06-27-2013
20130164799Integrated Process for the Production of Oil Bearing Chlorella Variabilis for Lipid Extraction Utilizing by Products of Jatropha Methyl Ester (JME) Production - An energy efficient process for the preparation of marine microalgae 06-27-2013
20130059350CONSTRUCTS AND METHODS FOR INCREASING YIELD OF FATTY ALCOHOLS IN CYANOBACTERIA - A construct containing a gene operably linked to a promoter having activity in cyanobacteria, and vector and cyanobacterium comprising such construct for increasing the yield of fatty alcohols in cyanobacterium is provided.03-07-2013
20110014665Production of Oil in Microorganisms - The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms containing one or more exogenous genes that facilitate the use of cellulosic materials as a feedstock. Also provided are microorganisms and methods for manufacturing non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils.01-20-2011
20130059351Thioesterase and a Method of Producing Fatty Acids or Lipids Using the Thioesterase - A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.03-07-2013
20130065283PROCESS FOR OBTAINING FATTY ACID ALKYL ESTERS FROM LIPIDS IN A MEMBRANE CONTACTOR - The present invention relates to a new process for obtaining fatty acid alkyl esters from fatty acid containing lipids, i.e. mono-, di- and triglycerides and phospholipids, in a membrane contactor.03-14-2013
20130065282Fermentation Process - Methods and systems for the production of one or more lipid products from a gaseous substrate using a two stage fermentation process are provided. The method comprises providing a gaseous substrate comprising CO03-14-2013
20090253188DELTA-4 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS - Described here are Δ4 desaturases that convert all-cis-7,10,13,16,19-docosapentaenoic acid [“DPA”; 22:5 ω-3] to docosahexaenoic acid [“DHA”; 22:6 ω-3], with secondary activity in converting docosatetraenoic acid [“DTA”; 22:4 ω-6] to all-cis-4,7,10,13,16-docosapentaenoic acid [“DPAn-6”; 22:5 ω-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding Δ4 desaturases as well as methods of making long chain polyunsaturated fatty acids [“PUFAs”] using this Δ4 desaturase in oleaginous yeast.10-08-2009
20090029428Novel microorganism, lipid-modifying agent, and the method of manufacturing 2-acyl lysophospholipids - The present invention provides a new supply source of phospholipase A01-29-2009
20090029427Increased Yield in Gas-to-Liquids Processing Via Conversion of Carbon Dioxide to Diesel Via Microalge - The present invention is generally directed to systems and methods for integrating gas-to-liquids (GTL) processing with biofuels production. In some embodiments of the present invention, carbon dioxide (CO01-29-2009
20090011480Use of Cellulosic Materials for Cultivation of Microorganisms - The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms that manufacture non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. Also provided are compositions comprising depolymerized cellulosic materials and oil-bearing microbes. Some methods of microbial fermentation are provided that comprise combining depolymerized cellulosic materials with other non-cellulosic feedstocks to enhance the economics of renewable fuel manufacturing. Particular advantages of the processes provided herein include production of oils rather than alcohols through cellulosic processes. Additional advantages include methods for manufacturing high nutrition oils from non-edible feedstocks such as wood chips, switchgrass, and bagasse.01-08-2009
20120237987BACTERIUM FOR PRODUCTION OF FATTY ACIDS - The present invention encompasses a bacterium that produces fatty acids.09-20-2012
20090042263Immobilized Enzymes and Methods of Using Thereof - The disclosed matter relates to immobilized enzymes and methods of use thereof.02-12-2009
20100297713MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENESIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ω-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ω-3, ETrA] to eicosatetraenoic acid [20:3 ω-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant Δ8 desaturases in plants and oleaginous yeast are disclosed.11-25-2010
20090197312Production of fat from alcohol - The present invention concerns a process for forming a lipid or a mixture of lipids from a starting material, which comprises at least one alcohol and a soap or a soap precursor, the process comprising adding a metal-ion forming agent to the starting material, whereby a mixture is formed, which contains an insoluble phase and a liquid phase, separating the insoluble phase from the liquid phase, contacting the lipid-producing microorganism with the liquid phase on a culturing substrate, whereby the microorganism cells begin producing lipid, and collecting the lipids. The present invention also concerns a process for forming a lipid or a mixture of lipids from an alcohol-containing liquid phase, which comprises at least one alcohol.08-06-2009
20100184169Modified Photosynthetic Microorganisms With Reduced Glycogen and Their Use in Producing Carbon-Based Products - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen as compared to a wild type 07-22-2010
20090209014HETEROTROPHIC ALGAL HIGH CELL DENSITY PRODUCTION METHOD AND SYSTEM - A multiphase culturing process for high density heterotrophic microalgal growth uses crude glycerol as the primary carbon source and produces ω-3 fatty acids. The process uses multiphase growth conditions that decouple the phases of increasing cell density and increasing cell size and fatty acid production. The entire process is integrated with biodiesel production.08-20-2009
20100255551MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacylglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.10-07-2010
20100267099DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed.10-21-2010
20130045515HETEROGENEOUS E. COLI FOR IMPROVING FATTY ACID CONTENT USING FATTY ACID BIOSYNTHESIS AND PREPARATION METHOD THEREOF - Disclosed are novel 02-21-2013
20110020883ACYL-ACP THIOESTERASE GENES AND USES THEREFOR - The present invention provides novel genes encoding Class II acyl-ACP thioesterases and variants thereof that are active on C8, C10, C12, C14, C16, and C18 acyl-ACP substrates. The thioesterases can be introduced into transgenic organisms, including microorganisms and photosynthetic organisms, for producing fatty acids and fatty acid products.01-27-2011
20110201066PRODUCTION OF FATTY ACID ALKYL ESTERS BY USE OF TWO LIPOLYTIC ENZYMES - A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride and alcohol is contacted with a first lipolytic enzyme having a relatively higher activity on free fatty acids than on triglyceride and a second lipolytic enzyme having a relatively higher activity on triglyceride than on free fatty acids.08-18-2011
20110287497ANAEROBIC ORGANISMS IN A PROCESS FOR CONVERTING BIOMASS - A method for integrating biological conversion of mixed acids to hydrocarbons, hydrocarbon-like molecules, biofuels, and combinations thereof. The method including introducing fermentation products to at least one microorganism chosen from the group consisting of heterotrophic microorganisms, photo-mixotrophic microorganisms, chemo-autotrophic microorganisms, and combinations thereof.11-24-2011
20110287496HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR BIOCATALYTIC SYNTHESIS OF STRUCTURED LIPIDS - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.11-24-2011
20110287495HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS TO PRODUCE TRIGLYCERIDES - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.11-24-2011
20110124063Methods, Systems, and Compositions for Microbial Bio-Production of Biomolecules Using Syngas Components, or Sugars, as Feedstocks - This invention relates to microorganism cells that are modified to increase conversion of carbon dioxide and/or carbon monoxide to a product, such as a fatty acid methyl ester, and to related methods and systems. A pathway from the Calvin Benson Cycle to the product is provided, which in various embodiments involves use of heterologous proteins that exhibit desired enzymatic conversions.05-26-2011
20090093033PUFA-PKS Genes From Ulkenia - The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.04-09-2009
20110294175INTEGRATED PROCESS FOR THE PRODUCTION OF BIO-OIL FROM MICRO-ORGANISMS - The cultivation of heterotrophic and phototrophic micro-organisms is integrated for the production of bio-oil for biofuels, wherein the overall algal suspension produced is first thickened, with recirculation of the excess water to cultivation containers, and then thermally treated at a high temperature. After cooling, a bio-oil phase is recovered together with a suspension rich in soluble carbohydrates and proteins which forms a nutritional/energy source for heterotrophic micro-organisms.12-01-2011
20110294174Tailored Oils Produced From Recombinant Heterotrophic Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.12-01-2011
20110294173Process and Microorganisms for Production of Lipids - The present invention provides a process for producing lipids for biofuel or lubricant and 12-01-2011
20100151540METHOD FOR PROCESSING AN ALGAE MEDIUM CONTAINING ALGAE MICROORGANISMS TO PRODUCE ALGAL OIL AND BY-PRODUCTS - A device and method for processing an algae medium containing algae microorganisms to produce algal oil and by-products thereof. The method comprises pumping the algae medium through a flow-through hydrodynamic cavitation device, generating localized zones of reduced fluid pressure, creating cavitational features in the algae medium, collapsing those cavitation features, and disintegrating cell walls and intracellular organelles to produce algal oil and by-products.06-17-2010
20100159540SYNTHESIS OF RESOLVINS AND INTERMEDIATES, COMPOUNDS PREPARED THEREBY, AND USES THEREOF - Methods are disclosed for the preparation of a new class of lipid mediators known as resolvins, with Resolvin D6 (4,17-dihydroxy-5E,7Z,10Z,13Z,15E,19Z-docosahexaenoic acid) being exemplary. Also disclosed are methods for the efficient synthesis of key intermediates in the preparation of such resolvins, such as isotopically labeled ω-3 fatty acid metabolites, and derivatives and analogs thereof. The invention likewise extends to the intermediates so prepared, and to the resolvins prepared with their use.06-24-2010
20090155864SYSTEMS, METHODS, AND DEVICES FOR EMPLOYING SOLAR ENERGY TO PRODUCE BIOFUELS - A photo-bioreactor can be arranged to receive incident solar radiation. The photo-bioreactor can contain photosynthetic organisms. The photosynthetic organisms can be genetically modified to produce an organic substance. The organic substance can be a biofuel or a precursor to a biofuel. The precursor can be isolated and converted into a biofuel. The biofuel can be extracted from the photo-bioreactor for use, for example, in energy generation or as a fuel.06-18-2009
20110217743Method of Producing Lauric Acid-containing Oil or Fat - To provide a method for supplying lauric acid with algae.09-08-2011
20120171735PRODUCTION OF TRIACYLGLYCERIDES, FATTY ACIDS, AND THEIR DERIVATIVES - The invention relates to production of triacylglycerols in cells.07-05-2012
20100112650POWDERY LIPASE PREPARATION, METHOD FOR PRODUCING THE SAME AND USE THEREOF - The present invention discloses a powdery lipase preparation which is a granulated material comprising a lipase derived from 05-06-2010
20090148918Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing - The invention provides methods of manufacturing biodiesel and other oil-based compounds using glycerol and combinations of glycerol and other feedstocks as an energy source in fermentation of oil-bearing microorganisms. Methods disclosed herein include processes for manufacturing high nutrition edible oils from non-food feedstock materials such as waste products from industrial waste transesterification processes. Also included are methods of increasing oil yields by temporally separating glycerol and other feedstocks during cultivation processes. Also provided herein are oil-bearing microbes containing exogenous oil production genes and methods of cultivating such microbes on glycerol and other feedstocks.06-11-2009
20100267098DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ49 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.10-21-2010
20100267097FIBROUS MATERIALS AND COMPOSITES - Fibrous materials, compositions that include fibrous materials, and uses of the fibrous materials and compositions are disclosed. For example, the fibrous materials can be operated on by a microorganism to produce ethanol or a by-product, such as a protein or lignin.10-21-2010
20100120103Recombinant Production Docosahexaenoic Acid (DHA) in Yeast - The present invention relates to a specifically novel recombinant method of production of the omega-3 fatty acid, Docosahexaenoic acid by a potentially safe recombinant organism 05-13-2010
20110151527PRODUCTION OF BIODIESEL, CELLULOSIC SUGARS, AND PEPTIDES FROM THE SIMULTANEOUS ESTERIFICATION AND ALCOHOLYSIS/HYDROLYSIS OF MATERIALS WITH OIL-CONTAINING SUBSTITUENTS INCLUDING PHOSPHOLIPIDS AND CELLULOSIC AND PEPTIDIC CONTENT - The present invention relates to a method for producing fatty acid alkyl esters as well as cellulosic simplified sugars, shortened protein polymers, amino acids, or combination thereof resulting from the simultaneous esterification and hydrolysis, alcoholysis, or both of algae and other oil containing materials containing phospholipids, free fatty acids (FFA), glycerides, or combination thereof as well as polysaccharides, cellulose, hemicellulose, lignocellulose, protein polymers, or combination thereof in the presence of an alcohol and an optional acid catalyst.06-23-2011
20110201063Algae growth for biofuels - A method and apparatus of algal oil production including the steps of: control growing to provide intensive growth to supply starting means for algae farming; Farming algae from using primarily sunlight Processing algae from the farmed algae preferably using a wet extraction process: and wherein at least one of the steps includes use of a bag able to be interconnected to gas or liquid flows of at least one of water, CO08-18-2011
20090142816GLIOCLADIUM ISOLATE C-13 AND METHODS OF ITS USE FOR PRODUCING VOLATILE COMPOUNDS AND HYDROCARBONS - Provided herein is 06-04-2009
20100099154PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF - The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.04-22-2010
20110201065SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELLS - The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.08-18-2011
20110201064CONVERSION TO BIOENERGY FROM BIOMASS OF PROTISTAN GRAZERS FEEDING ON AQUATIC PLANT AND/OR ALGAE WHICH CAN SURVIVE AND UPTAKE GREENHOUSE GASES IN THE MIXTURE OF TOXIC GASES AND SUBSTANCE - Provided are a method for removing and/or absorbing greenhouse gases using aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, a method for producing bioenergy using predators with high fatty acids, and a method for producing bioenergy including cultivating aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, while supplying a waste gas mixture to the aquatic plant and/or algae, (S2) cultivating predators having a capability to prey on the aquatic plant and/or algae while supplying the aquatic plant and/or algae to the predators, (S3) harvesting the predators, and (S4) converting the harvested predators into bioenergy.08-18-2011
20090286292PRODUCING EICOSAPENTAENOIC ACID (EPA) FROM BIODIESEL-DERIVED CRUDE GLYCEROL - , when cultured with crude glycerol as a carbon source, produces useful polyunsaturated fatty acids such as eicosapentaenoic acid (EPA). The crude glycerol is pretreated to remove soaps and methanol. An exemplary 11-19-2009
20090291479MANIPULATION OF ACYL-COA BINDING PROTEIN EXPRESSION FOR ALTERED LIPID PRODUCTION IN MICROBIAL HOSTS - Acyl-CoA binding protein [“ACBP”] binds thiol esters of long fatty acids and coenzyme A in a one-to-one binding mode with high specificity and affinity. This protein is expected to play an important role in altering lipid production in oleaginous microbial organisms. Knock-out of the protein in the oleaginous yeast, 11-26-2009
20090104673Process for Producing Triglycerides - A process for producing triglycerides comprises: (a) subjecting a first triglyceride comprising at least 40% by moles of oleic acid residues, based on total acyl groups in the triglyceride, to a reaction with stearic acid, at least one ester of stearic acid or a mixture thereof, to obtain a composition comprising 1,3-distearoyl 2-oleoyl glyceride and trioleoyl glyceride; (b) treating the composition to form a first fraction having an increased amount by weight of oleoyl groups compared to said composition and a second fraction having an increased amount by weight of stearoyl groups compared to said composition; (c) hydrolysing the first fraction to form oleic acid; and (d) reacting said oleic acid or an ester thereof with a triglyceride comprising at least 50% by moles of palmitic acid residues, based on total acyl groups, to form a composition comprising 1,3-dioleoyl 2-palmitoyl glyceride.04-23-2009
20090298142METHOD FOR PRODUCING A USEFUL SUBSTANCE BY USE OF AN IMMOBILIZED ENZYME - The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied therethrough. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform, to thereby facilitate production of a useful substance in an efficient manner.12-03-2009
20130217084PRODUCTION OF OMEGA-3 FATTY ACIDS FROM CRUDE GLYCEROL - The present invention relates to various methods to produce a variety of omega-3 fatty acids from various species of alga using crude glycerol as a substrate for algal growth. In one embodiment, the present invention relates to various methods to produced docosahexaenoic acid (DHA) from a 08-22-2013
20090170173NUCLEIC ACID MOLECULES ENCODING POLYPEPTIDES INVOLVED IN REGULATION OF SUGAR AND LIPID METABOLISM AND METHODS OF USE VIII - This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to 07-02-2009
20080274521DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ5 desaturases in oleaginous yeast are disclosed.11-06-2008
20090098622SCHIZOCHYTRIUM PKS GENES - The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of 04-16-2009
20090246839Enzymatic modification of triglyceride fats - Process wherein the fatty acid residues on a glyceride moiety are randomised over the terminal and middle positions, wherein the process proceeds to a conversion degree on the terminal positions, Re, ranging from 0.3 to 0.95, and wherein a conversion degree on the middle position, Ra, ranges from 0.06 to 0.75, and wherein Ra is greater than 0.32Re-0.08, the process comprises the exposure of a triglyceride fat to a catalyst comprising a lipase wherein the lipase is a Thermomyces lanuginosa lipase which has an activity of at least 250 IUN corresponding to 22 g/(g*h) at the onset of the process.10-01-2009
20130217085METHODS FOR IMPROVING FERMENTATION YIELD OF POLYUNSATURATED FATTY ACIDS - The present invention discloses a method of improving the fermentation yield of polyunsaturated fatty acids, in which 08-22-2013
20090061492Method for producing biodiesel - The present invention relates to the production of biodiesel. In particular, the present invention provides systems and methods for fermenting biomass materials with transgenic plant materials expressing the WRI1 transcription factor. In preferred embodiments, WRI1 is expressed in canola. The transgenic canola plants are fermented with a biomass source so that oil is produced using carbohydrates from the biomass source as an energy source.03-05-2009
20110229944FAD4, FAD5, FAD5-2, AND FAD6, Novel Fatty Acid Desaturase Family Members and Uses Thereof - The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.09-22-2011
20110223641MICROBIAL ENGINEERING FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES - Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.09-15-2011
20090117629ISOPRENOID WAX ESTER SYNTHASES, ISOPRENOID ACYL COA-SYNTHETASES, AND USES THEREOF - The present invention provides isolated polynucleotides and isolated polypeptides. The polypeptides of the present invention have isoprenoid wax ester synthase activity or isoprenoid acyl CoA-synthetase activity. The present invention also includes methods of using the polynucleotides and polypeptides of the present invention. For instance, the methods include producing biodiesel and producing wax esters.05-07-2009
20090209015COMPOSITIONS AND METHODS FOR PRODUCTION OF BIOFUELS - Provided is a method for producing C4 to C14 fatty acids. The method entails providing genetically modified cells that express a heterologous animal TE-II and either a heterologous animal wild type FAS or a ΔTE-I-FAS. The TE-II and the heterologous wild-type or ΔTE-I-FAS are expressed as distinct molecules. The cells are cultured for a period of time, and the fatty acids are extracted either as free fatty acids or as glycerides from the cells and/or from the media in which the cells are cultured.08-20-2009
20090246840PROCESS FOR PRODUCING ISOMER ENRICHED CONJUGATED LINOLEIC ACID COMPOSITIONS - A process for the preparation of a composition comprising the cis9, trans11 and trans10, cis12 isomers of conjugated linoleic acid (CLA) comprises: providing a mixture comprising cis9, trans11 and trans10, cis12 isomers of conjugated linoleic acid (CLA) in which one of the cis9, trans11 and trans10, cis12 isomers is present in a first weight ratio X of at least 1.3:1 with respect to the other isomer; and subjecting the mixture to crystallisation to form a composition comprising the cis9, trans11 and trans10, cis12 isomers in which one of the cis9, trans11 and trans10, cis12 isomers is present at a second weight ratio Y with respect to the other isomer, wherein Y is greater than X.10-01-2009
20120196337HETEROGENOUS ENZYMATIC CATALYST, PREPARATION METHOD, AND USE - The invention relates to a heterogenous enzymatic catalyst that takes the form of a cellular monolith consisting of a silica or organically modified silica matrix, said monolith including macropores, mesopores, and micropores, said pores being interconnected, and wherein the inner surface of the macropores is functionalized by a coupling agent selected from among silanes, said inner surface moreover having an unpurified enzyme attached thereon by means of a covalent or electrostatic bond. The invention also relates to the method for preparing said catalyst, said method comprising: a first step for preparing a solid silica impression that takes the form of a cellular monolith such as defined above; a second step for functionalizing the inner surface of the macropores via a coupling agent, selected from among silanes, by vacuum-soaking the cellular monolith by dissolving the coupling agent in an organic solvent; and a third step for vacuum-soaking the thus-functionalized monolith by means of an aqueous solution or aqueous dispersion of at least one unpurified enzyme. Finally, the invention relates to the use of such catalyst to carry out catalyzed chemical reactions.08-02-2012
20130122557YEAST STRAINS AND THEIR USES IN THE PRODUCTION OF LIPIDS - The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils.05-16-2013
20130122558MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b05-16-2013
20100261235CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD - A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by product glycerin can be automatically removed.10-14-2010
20100159539METHODS AND SYSTEMS FOR PRODUCING BIOFUELS AND BIOENERGY PRODUCTS FROM XENOBIOTIC COMPOUNDS - The present invention provides methods and systems for producing biofuel and bioenergy products using, as starting raw material, xenobiotic materials or compounds. The xenobiotic materials or compounds may originate from industrial or chemical plants, municipal waste, pharmaceutical products, cosmetic and personal care products, or other sources, and may include aliphatic and aromatic hydrocarbons, chlorinated organic solvents and other halogenated hydrocarbons, as well as heteroaromatic compounds. In accordance with the invention, these materials act as a carbon source to support the metabolism of xenobiotic-degrading microorganisms, thereby producing biomass and/or biogas that may be converted to bioenergy products by microbial synthesis. For example, the biomass may be converted to products such as ethanol, methanol, butanol, and methane, among others. The biogas may be converted to hydrogen gas and biodiesel, among others. Thus, the present invention couples the microbial breakdown (decomposition) of xenobiotic materials with the microbial synthesis of biofuel, thereby supplying needed (inexpensive) energy products, while reducing environmental pollution and contamination, and reducing the costs associated with disposal of hazardous waste.06-24-2010
20100159538METHOD FOR THE CHEMOENZYMATIC PRODUCTION OF FATTY ACID ESTERS - A chemoenzymatic process for the production of fatty acid esters comprising partially esterifying one or more fatty acid esters, under mild temperatures with an enzymatic catalyst, and optionally in the presence of one or more inert solvents, with a 1- to 5-fold molar excess of one or more water-containing aliphatic alcohols with boiling points between 60° C. and 120° C., then removing the water and unreacted alcohol(s) from the resulting pre-esterification product, followed by additional esterification up to 99.7%, chemically-catalyzed with, e.g., an acid or tin salt at slightly higher temperatures, optionally using one or more inert solvents, with a 1- to 4-fold molar excess of the same one or more aliphatic alcohols as employed in the preliminary esterification step.06-24-2010
20110059495MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS - Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus 03-10-2011
20110059494D4 DESATURASES AND D5 ELONGASES - Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them.03-10-2011
20120196336RADIAL COUNTERFLOW REACTOR WITH APPLIED RADIANT ENERGY - An improvement is described for the processing of biological material in a continuous stream by the application of radiant energy taken from the wavelengths from infrared to ultraviolet, and its absorption by a feedstock in a workspace of featuring controlled turbulence created by one or more counter-rotating disk impellers. The absorbed energy and the controlled turbulence patterns create a continuous process of productive change in a feed into the reactor, with separated light and heavy product output streams flowing both inward and outward from the axis in radial counterflow. The basic mechanism of processing can be applied to a wide range of feedstocks, from the promotion of the growth of algae to make biofuel or other forms of aquaculture, to a use in the controlled combustion of organic material to make biochar.08-02-2012
20100216198METHOD FOR THE SYNTHESIS OF OMEGA-UNSATURATED FATTY ACIDS - The invention relates to a method for the synthesis of short-chain ω-unsaturated fatty acids of general formula CH08-26-2010
20100255550Regulatory Factors Controlling Oil Biosynthesis In Microalgae And Their Use - The production of transgenic plant oils is described by transfecting plant host cells with heterologous transcription inducers. Such inducers are specific for endogenous biosynthetic plant oil genes, such that the inducers induce an overexpression of the plant oil genes. The overexpressed gene product (i.e., for example, diacylglycerol transferase) thereby results in a increased intracellular production of a plant oil (i.e., for example, triacylglycerol). In one embodiment, the transfected plant host cell can be a plant algae species (i.e., for example, 10-07-2010
20130217082Algae Biofuel Carbon Dioxide Distribution System - A closed-loop system for growing algae in a bioreactor is disclosed, for example, to produce biofuel. An aqueous bioreactor solution is formulated such that the principal source of carbon for algae growth is supplied by sodium bicarbonate. During algae growth in the aqueous solution, the concentration of sodium bicarbonate in the solution is reduced while the concentration of sodium carbonate increases. A regenerator is provided to regenerate sodium bicarbonate from the sodium carbonate. Specifically, after sufficient growth, the algae are concentrated and an algae depleted media is produced. Carbon dioxide is then introduced into the algae depleted media to regenerate sodium bicarbonate from the sodium carbonate. The regenerated sodium bicarbonate is then recycled into the bioreactor to supply carbon for further algae growth.08-22-2013
20130217083GERMINATING ZYGOSPORES OF UNICELLULAR, SPORE-FORMING ALGAE AS A SOURCE OF ABUNDANT EXTRACTABLE LIPID - Described are procedures that promote the accumulation and release of intracellular lipid bodies from zygospores of single-celled alga. This disclosure describes procedures for manipulating the progression or synchronization of the algae's life cycle, and for simplifying lipid extraction by promoting spontaneous release of intracellular lipid reserves. The approach is relevant to the commercial production of biodiesel and food supplements derived from fatty acids.08-22-2013
20100035312IMMOBILIZED INTERFACIAL ENZYMES OF IMPROVED AND STABILIZED ACTIVITY - Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.02-11-2010
20120070870Methods and Molecules for Yield Improvement Involving Metabolic Engineering - The invention features methods and compositions relating to cells that have been engineered to reduce or eliminate proteins having enzymatic activity that interfere with the expression of a metabolic product.03-22-2012
20090075349METHOD OF RECOVERING LIPASE ACTIVITY - The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from 03-19-2009
20120034662CO-CULTURING ALGAL STRAINS TO PRODUCE FATTY ACIDS OR HYDROCARBONS - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons.02-09-2012
20100196971SEQUESTRATION OF COMPOUNDS FROM MICROORGANISMS - The invention provides novel mesoporous particles and methods for the selective sequestration of organic compounds from microorganisms, such as from various genera and species of algae that produce important organic compounds. The organic compounds can be selectively sequestered, for example, in favor of biodiesel impurities such as sterols and chlorophyll, to provide substantially pure free fatty acids. The free fatty acids can then be esterified to provide pure biodiesel.08-05-2010
20110151524PROCESS FOR PRODUCING BIODIESEL THROUGH LOWER MOLECULAR WEIGHT ALCOHOL-TARGETED CAVITATION - A method for producing fatty acid alkyl esters from biolipids through transesterification and/or esterification reactions uses a flow-through cavitation device for generating cavitation bubbles in a fluidic reaction medium. The fluidic medium is passed through sequential compartments in the cavitation device having varying diameters and inner surface features to create localized reductions in fluid pressure thus vaporizing volatile alcohols and creating an increased surface area and optimized conditions for the reaction to occur at the gas-liquid interface around the bubbles.06-23-2011
20130130335Purification Methods and Systems Related to Renewable Materials and Biofuels Production - Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation.05-23-2013
20090148919Delta 6-Desaturase Genes and Uses Thereof - The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 6 (i.e., “Δ6-desaturase”). In particular, Δ6-desaturase may be utilized, for example, in the conversion of linoleic acid to γ-linolenic acid and in the conversion of α-linolenic acid stearidonic acid. The polyunsaturated fatty acids produced by use of the enzyme may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.06-11-2009
20100323413Production of Oil in Microorganisms - The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sucrose feedstocks from sugar cane, sugar beets, and molasses for bioproduction processes. The disclosed processes utilize oleaginous microbes as a conversion technology to convert chemical energy produced by sugar cane, molasses and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oleaginous microbes containing one or more exogenous sucrose utilization genes.12-23-2010
20100081177Decreasing RUBISCO content of algae and cyanobacteria cultivated in high carbon dioxide - Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO04-01-2010
20110129884METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE ORDER THRAUSTOCHYTRIALES - The present invention relates generally to the field of microorganism cell culture. It devises a cell culture medium for and a method of culturing microorganisms of the order Thraustochytriales, especially for the production of omega-3-fatty acids. Furthermore it devises the use of ammonium tartrate as nitrogen source for the cultivation of micro-organisms of the order Thraustochytriales.06-02-2011
20100151538Cellulosic Cultivation of Oleaginous Microorganisms - Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, 06-17-2010
20100151539Recombinant Microalgae Cells Producing Novel Oils - Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.06-17-2010
20110244532PRODUCTION OF BRANCHED CHAIN FATTY ACIDS AND DERIVATIVES THEREOF IN RECOMBINANT MICROBIAL CELLS - Recombinant microbial cells are provided which have been engineered to produce branched chain products such as branched fatty acid derivatives by the fatty acid biosynthetic pathway, and methods of making branched fatty acid derivatives using the recombinant microbial cells.10-06-2011
20120244584ENGINEERING MICROORGANISMS - The present disclosure provides, inter alia, 09-27-2012
20100062502MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS - Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast 03-11-2010
20100047883PROCESS FOR PRODUCING USEFUL SUBSTANCE USING IMMOBILIZED ENZYME - The present invention relates to a process for producing a useful substance, the process including feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction, wherein the packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mmφ or larger is 10 to 200 mm.02-25-2010
20100062501METHOD - The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.03-11-2010
20110097771ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING PLANT UTILITY - Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant.04-28-2011
20100068772Solubilization of algae and algal materials - Methods for solubilizing algae or algal material are provided to facilitate the recovery of oil or lipids, as well as hydrocarbons and carbohydrates, from algae or algal material. The methods involve contacting algae or algal material with an oxoacid ester or thioacid ester of phosphorus or a mixture of an oxoacid of phosphorus and/or an alcohol to form a mixture thereof under conditions effective to solubilize the algae or algal material. These methods optionally further comprise bioconversion of the solubilized algae or algal material to form a composition suitable for recovery of oils and non-oil chemicals.03-18-2010
20110081691GENE THAT INCREASES PRODUCTION OF PLANT FAT-AND-OIL AND METHOD FOR USING THE SAME - This invention is intended to search for a transcription regulator having a novel function of improving the amount of substances produced per individual plant and to enhance such properties in a plant. A chimeric protein resulting from the fusion of a transcription factor belonging a transcription factor family that includes a transcription factor comprising the amino acid sequence as shown in SEQ ID NO: 4 with the repressor domain is expressed in a plant.04-07-2011
20110097770Novel method to generate bioactive compounds in algae - The present invention discloses a novel microalgae-based method, called Microalgae Genomics Technology™ (MaGT), which can be used to generate novel compounds or increase the existing compounds in algae, and revealed some example of its potential applications. Specifically, this method utilizes microalgae genomics technology to manipulate the metabolic pathways in the genomes of microalgae and therefore induce the algae to produce novel secondary compounds or increase the concentration of existing compounds with a variety of commercial applications.04-28-2011
20110097769MODIFIED MICROORGANISM USES THEREFOR - The invention provides a genetically modified microorganism that acquires the ability to consume a renewable feedstock (such as cellulose) and produce products. This organism can be used to ferment cellulose, one of the most abundant renewable resources available, and produce products.04-28-2011
20110250659MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS - This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that contain one or more exogenous genes encoding a phospholipase and/or thioesterase, which are capable of producing an increased amount of lipids and/or fatty acids. This disclosure also describes genetically modified photosynthetic microorganisms that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides, as well as photosynthetic microorganism comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism.10-13-2011
20110151526Branched-Chain Fatty Acids And Biological Production Thereof - A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to β-methyl-D-malate; conversion of β-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to α-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided.06-23-2011
20110059496GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST - Promoter regions associated with the 03-10-2011
20120149075SOAPS PRODUCED FROM OIL-BEARING MICROBIAL BIOMASS AND OILS - Soap and cosmetic products can be made from oil-bearing microbial biomass via the alkaline hydrolysis of glycerolipids and fatty acid esters to fatty acid salts. The saponified microbial oils/lipids can be combined with a variety of additives to produce compositions for use as soaps and other cosmetic products, which may also contain other constituents of the biomass, including unsaponified oils, glycerol and carotenoids, among others.06-14-2012
20120149074Transformant Comprising Gene Coding for ws/dgat and Method of Producing Fatty Acid Ethyl Esters Using the Same - The present invention relates to a transformant comprising a gene encoding ws/dgat (wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase) and a method of producing fatty acid ethyl esters using the same. Specifically, the transformant for producing fatty acid ethyl esters is constructed such that glycerol is used as a fermentation substrate, and comprises an atfA gene encoding ws/dgat (wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase) from 06-14-2012
20100304452PROCESS OF PRODUCING OIL FROM ALGAE USING BIOLOGICAL RUPTURING - A process for production of biofuels from algae can include cultivating an oil-producing algae, extracting the algal oil, and converting the algal oil to form biodiesel. Extracting the algal oil from the oil-producing algae can include biologically rupturing cell wall and oil vesicles of the oil-producing algae using at least one enzyme such as a cellulose or glycoproteinase, a structured enzyme system such as a cellulosome, a virus, or combination of these materials.12-02-2010
20110250658Recombinant Microalgae Cells Producing Novel Oils - Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.10-13-2011
20120171736POWDERY LIPASE PREPARATION AND USE THEREOF - The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm07-05-2012
20090317878NUCLEAR BASED EXPRESSION OF GENES FOR PRODUCTION OF BIOFUELS AND PROCESS CO-PRODUCTS IN ALGAE - Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain nuclear-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, starches, raw materials, pharmaceuticals or fine chemicals.12-24-2009
20110151525ENZYMATIC PRODUCTION OF UNSATURATED FATTY ACIDS ESTERS OF ASCORBIC ACID IN SOLVENT-FREE SYSTEM - The invention provides a cost-effective enzymatic process for the production of unsaturated FA esters of ascorbic acid, such as ascorbyl oleate or ascorbyl linoleate (E-304), in solvent-free system in which ascorbic acid esters of saturated FA, unsaturated free fatty acids and/or unsaturated fatty acid esters are used as feedstock. With this method pure products can even be achieved in a short response times. These products meet the needs of the industry by improving their solubility in oil and the increased antioxidant activity, especially in foods, cosmetics and medicine.06-23-2011
20080254521DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed.10-16-2008
20080254522DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.10-16-2008
20110256594REMOVAL OF NITROGEN FROM A CHLOROPHYLL OR PHEOPHYTIN CONTAINING BIOMASS - The present disclosure relates to refining a product from a biomass containing chlorophyll and/or pheophytins. In particular, a method of refining a product (such as a biofuel) from a photosynthetic organism is disclosed. The photosynthetic organism can be a naturally occurring organism or a genetically modified or altered organism. The method of refining comprises removing nitrogen to obtain the desired product. In some aspects, nitrogen is removed from a chlorophyll and/or pheophytin containing product by enzymatic degradation of chlorophyll and/or pheophytins and subsequent removal of the nitrogen10-20-2011
20110053229METHOD FOR PRODUCTION OF IMMOBILIZED ENZYME - Provided is a method of producing an immobilized lipolytic enzyme which has almost the same ability as that of an unused immobilized enzyme by effectively utilizing an immobilization carrier in a used immobilized enzyme which has been used in an esterification reaction.03-03-2011
20100285545BIOSYNTHETIC ROUTES TO LONG-CHAIN ALPHA,OMEGA-HYDROXYACIDS, DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS - A substantially pure 11-11-2010
20110136189Integrated Processes and Systems for Production of Biofuels Using Algae - A process for production of biofuels from biomass can include depolymerizing a biomass to form a feed. The feed can be formed by autotrophically growing algal biomass and extracting the feed therefrom. The algal feed can be converted to an algal oil by introducing the algal feed to an oil-producing algae under growth conditions sufficient to encourage formation of algal oil within the oil-producing algae. The algal oil can be extracted from the oil-producing algae and can further be converted to biodiesel.06-09-2011
20110136187Process - A process of water degumming an edible oil (preferably a crude edible oil) comprising the steps of: a) admixing approximately 0.1-5% w/w water with an edible oil (preferably a crude edible oil) and a lipid acyltransferase, b) agitating the admixture for between about 10 minutes and 180 minutes at about 45 to about 900 C, and c) separating the oil phase and the gum phase. Preferably said lipid acyltransferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ No. 49; and/or is a polypeptide having lipid acyltransferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith. In one embodiment the lipid acyltransferase is preferably used in combination with a phospholipase C enzyme. A process for modifying the gum phase of a degummed oil using a lipid acyltransferase is also taught herein.06-09-2011
20110008854PROCESS FOR THE GENERATION OF ALGAL OIL AND ELECTRICITY FROM HUMAN AND ANIMAL WASTE, AND OTHER HYDROCARBON SOURCES - A method for the generation of electricity includes producing a paste from hydrocarbon waste, delivering the paste to a pyrolysis unit, rendering the paste into a gaseous product stream, allowing the stream to flow through a suitable filter device, extracting energy from the stream, producing electricity, converting water to hydrogen via a water gas shift reaction, allowing the reacted stream to flow to a reciprocating compressor, allowing any remaining stream to pass through compression, cooling, condensing, and storing carbon dioxide in a high pressure tank, allowing any remaining stream to be split, one part being combusted in a gas turbine producing electricity and the other part being recycled to the plasmolysis unit, injecting exhaust gas into a feed stream which flows to a degasification chamber, and allowing carbon dioxide and residual water from a storage tank to be expanded, passed through the heat exchanger and injected into the aquaculture feed.01-13-2011
20100330629ROBUST MULTI-ENZYME PREPARATION FOR THE SYNTHESIS OF FATTY ACID ALKYL ESTERS - Disclosed is an enzymatic process for the preparation of fatty acid alkyl esters, particularly fatty acids methyl esters (biodiesel) in a solvent-free microaqueous system, from a fatty acid source and an alcohol or alcohol donor, employing a robust lipase preparation that comprises at least two lipases separately or jointly immobilized on a suitable support, where one of the lipases has increased affinity to partial glycerides, another is sn-1,3 positional specific, and an optional third lipase has high selectivity towards sn-2 position of the glycerol backbone of the fatty acid source.12-30-2010
20100330628ACTIVATED LIPASES AND METHODS OF USE THEREFOR - Methods for enhancing a biological (e.g., catalytic) activity of a lipase, are provided. In some embodiments, the methods include the step of alkylating one or more cysteine residues present within the lipase. Also provided are modified polypeptides for which a biological activity is enhanced by the disclosed methods, methods for using the disclosed polypeptides, including for the transesterification of renewable oils to produce a biofuel, and cell free systems that include a lipase, to which one or more moieties, such as steroidal moieties, are conjugated.12-30-2010
20090203092Liquid Biofuel Mixture and Method and Device for Producing The Same - The present invention relates to a biofuel mixture comprising a fraction of fatty acid alkyl esters and at least one fraction of bound glycerol in an amount of ≧1 wt %, based on the glycerol backbone, and a method and a device for producing said biofuel mixture.08-13-2009
20090203093Protein Targeting To Lipid Bodies - The present invention relates to a method of targeting a protein of interest to an intracellular hydrophobic inclusion body of a bacterial cell by means of a fusion protein comprising a hydrophobic targeting peptide operatively linked with said protein of interest; methods of microbial production of a lipophilic compound of interest by means of a recombinant bacterial host comprising intracellular inclusion bodies having at least one enzyme which is involved in the biosynthesis of said lipophilic compound targeted to said inclusion bodies; as well as corresponding fusion proteins, coding sequences, expression vectors and recombinant hosts.08-13-2009
20090176284PROCESS FOR PREPARING CONCENTRATED POLYUNSATURATED FATTY ACID OIL - The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.07-09-2009
20090176285Compositions and Methods for Enhancing Glycerol Utilization - A glycerol utilizing cell and a method for the production of glycerol-derived target compounds are provided. The glycerol utilizing cell may comprise a glycerol metabolizing system or a glycerol uptake protein and be used to produce a glycerol-derivable target compound.07-09-2009
20120309064MUTANT DELTA-5 DESATURASES MUTATED IN THE HEME-BINDING MOTIF (HPGG) AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochome b12-06-2012
20120309063DETERGENT COMPOSITIONS CONTAINING GEOBACILLUS STEAROTHERMOPHILUS LIPASE AND METHODS OF USE THEREOF - The present compositions and methods relate to a lipase cloned from 12-06-2012
20110189741PROCESS AND APPARATUS FOR EXTRACTING BIODIESEL FROM ALGAE - The present invention relates to a system for cultivation of algae, extraction of lipids and transesterification of the lipids to obtain biodiesel. The system comprises three sections, that is to say cultivation, extraction and storage and reaction. In the lipid extraction area there is an ultrasonic reactor wherein the external walls of the alga are ruptured together with those of the oil sac to permit the extraction of lipids in the transesterification area there is also an ultrasonic reactor which ruptures the molecules of the fluid which passes therethrough to accelerate the reaction and render it almost immediate.08-04-2011
20110136188SYNTHESIS OF FATTY ALCOHOL ESTERS OF ALPHA-HYDROXY CARBOXYLIC ACIDS AND THEIR USE AS PERCUTANEOUS ABSORPTION ENHANCERS - The present invention provides a novel approach for the preparation of fatty alcohol esters of α-hydroxy carboxylic acids. In one form of the invention, the target fatty alcohol ester of α-hydroxy carboxylic acid is produced by converting a lower alkyl ester of α-hydroxy carboxylic acid into a fatty alcohol ester of α-hydroxy carboxylic acid via alcoholysis (i.e., transesterification). The transesterification process is an equilibrium reaction, catalyzed chemically (i.e., with acids or bases) or enzymatically, that is shifted in the desired direction to produce the desired product. One preferred way of shifting the reaction in the direction of the desired product is by reducing the concentration of one of the products (e.g., distillation of a lower-boiling alcohol as soon as it is formed). Another preferred way of shifting the reaction in the direction of the desired product is by increasing the concentration of one of the reactants (e.g., adding more of the starting ester).06-09-2011
20100028961Biogical production of fuels - A process for the production of one or more fatty acids or derivatives thereof from a carbohydrate, which process comprises treating of Jerusalem Artichoke the carbohydrate with a micro-organism which converts the carbohydrate to a microbial oil comprising one or more fatty acids or derivatives thereof, in which the carbohydrate is inulin and/or is derived from Jerusalem Arti-choke.02-04-2010
20110027842Method for Producing Monosaturated Glycerides - A process for producing a glyceride product which is enriched in monounsaturated fatty acids relative to the starting glyceride comprising the steps: (a) alcoholysis of triglycerides employing lipolytic enzymes selective for saturated fatty acids and/or lipolytic enzymes selective for the 1-position, the 3-position or both positions in a glyceride; and (b) separation of fraction A which is enriched in saturated fatty acid esters from fraction B which is enriched in monounsaturated glycerides.02-03-2011
20100062500METHOD FOR THE REESTERIFICATION OR ESTERIFICATION OF SIDE CHAINS IN POLYMERS - The present invention relates to a process for esterifying or transesterifying side chains in polymers, comprising the following steps 03-11-2010
20100081178MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacylglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.04-01-2010
20100028962Algal Medium Chain Length Fatty Acids and Hydrocarbons - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons.02-04-2010
20110027841METHOD FOR PREPARATION OF OXYLIPINS - The invention provides methods of producing oxygenated derivatives of polyunsaturated fatty acids (PUFAs).02-03-2011
20090298143SECRETION OF FATTY ACIDS BY PHOTOSYNTHETIC MICROORGANISMS - Recombinant photosynthetic microorganisms that convert inorganic carbon to secreted fatty acids are described. Methods to recover the secreted fatty acids from the culture medium without the need for cell harvesting are also described.12-03-2009
20100190220METHOD FOR PRODUCING EPA-ENRICHED OIL AND DHA-ENRICHED OIL - Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.07-29-2010
20110306101 METHOD OF PRODUCING FATTY ACIDS FOR BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALS - A method of producing fatty acids, by: (i) inoculating in a first bio-reactor a mixture of at least one of cellulose, hemicellulose, and lignin with a microorganism strain that produces one or more cellulases, hemicellulases, and/or laccases that hydrolyze at least one of cellulose, hemicellulose and lignin, and culturing the mixture to produce at least one fermentation product, for example one or more alcohols, in the mixture; (ii) optionally removing a portion of the at least one fermentation product from the mixture; (iii) transferring the remaining portion of the mixture into a second bio-reactor; and (iv) inoculating the portion of the mixture in the second bio-reactor with an algae strain that is capable of metabolizing the at least one fermentation product and any of the remaining soluble sugars, and culturing the mixture under conditions so that the at least one algae strain produces one or more fatty acids.12-15-2011
20110306102IMPROVED METHODS FOR FERMENTATIVE PRODUCTION OF DOCOSAHEXAENOIC ACID - The invention comprises a process of producing polyunsaturated fatty acids from Thraustochytriales at high availability of oxygen by solid state fermentation or submerged fermentation in a batch, semi-continuous or continuous mode. Batch fermentation of more than 200 g/L and up to 450 g/L of dextrose added right in the beginning of fermentation was achieved with productivity of DHA achieved for higher than 10 g/Liter. The process comprises maintaining at least 20% to 30% dissolved oxygen and sometimes up to around 50% or more.12-15-2011
20110091945METHODS OF INCREASING BIOMASS PRODUCTIVITY, LIPID INDUCTION, AND CONTROLLING METABOLITES IN ALGAE FOR PRODUCTION OF BIOFUELS USING BIOCHEMICAL STIMULANTS - The present disclosure provides methods of enhancing the biofuel potential of an algal culture, the ability of an algal culture to provide a biofuel such as a lipid or to be processed to a biofuel, the method comprising: contacting an algal culture with a composition selected to enhance the biofuel potential of an algal culture; and allowing the algal culture to incubate to the point where the potential of the algal culture to provide a biofuel product or be processed to a biofuel product is enhanced compared to when the algal culture is not in contact with the composition. The selected algal species can be a species of a genus selected from the group consisting of: 04-21-2011
20100086979PRODUCTION OF OMEGA-3 FATTY ACIDS IN MICROFLORA OF THRAUSTOCHYTRIALES USING MODIFIED MEDIA - The present invention relates to improved methods for the production of omega-3 fatty acids by microorganisms belonging to the microflora of Thraustochytriales using a modified composition of the culture medium. In particular, the invention is directed to a process for the production of omega-3 fatty acids by culturing microflora, such as strains of 04-08-2010
20100112649COMPOSITIONS, METHODS AND USES FOR GROWTH OF MICROORGANISMS AND PRODUCTION OF THEIR PRODUCTS - Embodiments of the present invention report compositions, methods and uses for growth and/or production of byproducts, products and/or co-products from algal cultures. Certain embodiments of the present invention report using wastewaters to grow microorganisms for producing one or more products, byproducts or co-products from the microorganisms. Other embodiments report compositions and methods for rapid growth of any photosynthetic organism in culture.05-06-2010
20110111470Methods and Compositions for the Recombinant Biosynthesis of Fatty Acids and Esters - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.05-12-2011
20100240106MUTANT ENZYMES - This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes.09-23-2010
20120301932EXPRESSION OF CALEOSIN IN RECOMBINANT OLEAGINOUS MICROORGANISMS TO INCREASE OIL CONTENT THEREIN - Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.11-29-2012
20090137013MICROORGANISMS AND METHODS FOR INCREASED HYDROGEN PRODUCTION USING DIVERSE CARBONACEOUS FEEDSTOCK AND HIGHLY ABSORPTIVE MATERIALS - The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed 05-28-2009
20120040419METHOD FOR PRODUCING OIL BY CYSTOFILOBASIDIUM SPP. - An oil generation system is provided. The oil generation system includes a saccharide; and a working microorganism reacting with the saccharide to produce an oil, wherein the working microorganism has a genus being a 02-16-2012
20120045800Microalgae Biofuel Production System - A system for processing oil from algae is disclosed. Specifically, the system recycles byproducts of the process for use as nutrients during algae growth and oil production. The system includes a conduit for growing algae and an algae separator that removes the algae from the conduit. Also, the system includes a device for lysing the algae and an oil separator to remove the oil from the lysed matter. Further, the system includes a biofuel reactor that receives oil from the oil separator and synthesizes biofuel and glycerin. Moreover, the algae separator, oil separator and biofuel reactor all recycle byproducts back to the conduit to support further algae growth.02-23-2012
20100323414Production of Oil in Microorganisms - The invention provides methods of producing lipids by oleaginous microorganisms. The invention also provides genetically engineered oleaginous microorganisms and methods of cultivation for lipid production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured such microorganisms. Exemplary oleaginous microorganisms include oleaginous fungi and such oleaginous fungi that have been genetically modified.12-23-2010
20120115193TRANSFORMATION OF GLYCEROL AND CELLULOSIC MATERIALS INTO HIGH ENERGY FUELS - The present invention includes a system and method for making a biofuel comprising: providing a nitrogen-limiting, minimal growth media comprising glycerol, sugars generated from cellulosic biomass or both, under conditions in which an oleaginous microbe converts the growth media into at least one of triacylglycerol, neutral lipids, fatty acids, long-chain fatty acids, and hydrocarbons that is secreted by the microbe.05-10-2012
20120208246PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION - An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.08-16-2012
20120015413Microorganisms with Extended Substrate Utilization Range - The present invention relates to a microorganism of the genus 01-19-2012
20110165639REFINERY PROCESS TO PRODUCE BIOFUELS AND BIOENERGY PRODUCTS FROM HOME AND MUNICIPAL SOLID WASTE - The present invention provides methods of a process (SOLWASFUEL system) for producing biofuel and bioenergy products using, as starting raw material, home and municipal organic solid waste, including recalcitrant lignocellulosic materials of paper, cardboards, organic plastics, cellulose plants, and food waste. In accordance with the invention, home and municipal solid waste, can serve as a carbon source to support the metabolism of synthetic microorganisms to produce biofuels and bioenergy products.07-07-2011
20110165638Integrated Bio-Digestion Facility - Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester.07-07-2011
20110165637FATTY ACID-PRODUCING HOSTS - Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally deleted. The hosts preferably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recombinantly stable and growth-competent at 37° C. Methods of producing a fatty acid product comprising culturing such hosts at 37° C. are also described.07-07-2011
20120058525METHOD FOR PRODUCING OIL BY YEAST - A method for producing oil by using yeast is provided. The method comprises utilizing at least one yeast strain serving as a working microorganism to produce the oil, wherein the yeast strain is genus 03-08-2012
20120058527CAL A-RELATED ACYLTRANSFERASES AND METHODS OF USE, THEREOF - Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions.03-08-2012
20120058526Methods and Systems for Processing Sugar Mixtures and Resultant Compositions - A method including: (a) selectively reacting a first sugar in a mixture which includes at least one second sugar to form a product mixture comprising a product of said first sugar; (b) separating said product of said first sugar from said product mixture; and (c) separating at least one of said at least one second sugar from said product mixture.03-08-2012
20120208247Non-Dispersive Process for Insoluble Oil Recovery from Aqueous Slurries - The development and application of a novel non-polar oil recovery process utilizing a non-dispersive solvent extraction method to coalesce and recover oil from a bio-cellular aqueous slurry is described herein. The process could apply to recovery of algal oil from a lysed or non-lysed algae slurry, recovery of Omega fatty acids from a bio-cellular aqueous feed, recovery of Beta-carotene from a bio-cellular aqueous feed and for the removal from produced water in oil production and similar type applications. The technique of the present invention utilizes a microporous hollow fiber (MHF) membrane contactor. The non-polar oil recovery process described herein can be coupled to a collecting fluid (a non-polar solvent such as heptane, a biodiesel mixture or the previously extracted oil) that is circulated through the hollow fiber membrane. In cases where the biodiesel mixture or the previously extracted oil is used the solvent recovery step (e.g. distillation) can be eliminated.08-16-2012
20120070869Methods of using Nannochloropsis Algal strains to produce hydrocarbons and fatty acids - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a 03-22-2012
20110091947High-Purity Purification Method for Omega-3 Highly Unsaturated Fatty Acids - The present invention relates a high-purity purification method for omega-3 highly unsaturated fatty acids. More specifically, it relates to a high-purity purification method for omega-3 highly unsaturated fatty acids which is both environmentally friendly and easy to implement and comprises the steps of: a) preparing a fatty acid ethyl ester (FAEE) by ethanolysis of a natural oil or fat, using ethanol, in the presence of an enzyme catalyst extracted from at least one microorganism selected from the group consisting of 04-21-2011
20110091946Enzymatic Process for Obtaining a Fatty Ester - The aim of the present invention is to obtain fatty esters from fatty acids derived from vegetable oils and butters, through an enzymatic process, so that said esters can act as emollients, emulsifiers and co-emulsifiers with differentiated performance, having high spreadability and slidability compared to the prior-art products. More specifically, the present invention relates to an enzymatic process for obtaining a fatty ester, comprising: a) obtaining a fatty acid through the enzymatic hydrolysis (saponification) of vegetable oils and butters selected from cupuagu butter, sapucainha butter, ucu{acute over (υ)}ba butter, murumuru butter, palm olein, pataua oil, tucuma oil, inaja oil, passion fruit oil, pequi oil and nasturtium oil; b) reacting the fatty acid with a fatty alcohol selected from capric alcohol, caprylic alcohol, isoamylic alcohol, lauric alcohol, myristic alcohol, lorol (70:30 mixture of lauric alcohol and myristic alcohol), cetyl alcohol, stearyl alcohol, cetostearyl alcohol (70:30 mixture of cetyl alcohol and stearyl alcohol), sorbitol, sorbitan, glycerin, polyglycerin, other polyols and mixture thereof, in the presence of an enzyme, in at least one reactor, at a temperature of from 40 to 80 QC; c) stirring the reaction solution; d) removing moisture from the reaction medium; e) vacuum filtering said solution to obtain the fatty ester. The fatty esters obtained through the process of the present invention can be used in the preparation of cosmetic compositions.04-21-2011
20080199923Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids - Disclosed is a process for growing the microflora 08-21-2008
20100093047MICROBIAL PROCESSING OF CELLULOSIC FEEDSTOCKS FOR FUEL - A system and method are provided which utilize microbes to convert biomass feedstock into a fuel. In one aspect, a method of producing lipids includes receiving a feedstock including biomass, exposing the feedstock to microbes which are capable of converting the feedstock into lipids, and extracting produced lipids.04-15-2010
20120122164METHOD AND SYSTEM FOR PROCESSING A BIOMASS FOR PRODUCING BIOFUELS AND OTHER PRODUCTS - The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention.05-17-2012
20110003351DELTA-5 DESATURASE AND USES THEREOF - The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “Δ5-desaturase”) and at carbon 6 (i.e., “Δ6-desaturase”) and to uses thereof. In particular, Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to γ-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.01-06-2011
20090130728Process for Producing a Triglyceride - A process for the production of a composition comprising 1,3-dioleyl-2-palmitoyl glyceride (OPO) comprises subjecting a palm oil stearin, with an iodine value (IV) between about 2 and about 12 to enzymic transesterification, with oleic acid or a non-glyceride ester thereof.05-21-2009
20110183387New Mutant Yeast Strains Capable of Accumulating a Large Quantity of Lipids - The subject of the present invention is a novel mutant yeast strain, particularly a 07-28-2011
20100209982MODIFIED-IMMOBILIZED ENZYMES OF HIGH TOLERANCE TO HYDROPHILIC SUBSTRATES IN ORGANIC MEDIA - Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.08-19-2010
20120214212Expression and application of recombinant FAEES in algae as a novel method to produce biodiesel in vivo - This discovery is related to the production of bioenergy fuels in vivo, specifically related to the production of biodiesel fuels using algae. We express FAEES (fatty acid ethyl ester synthase) in algae, and use the recombinant FAEES (fatty acid ethyl ester synthase) in the algae to synthesize biodiesel, fatty acid ethyl ester or fatty acid methyl ester, in vivo from fatty acids in the presence of ethanol/or methanol.08-23-2012
20120135479FRACTIONATION OF OIL-BEARING MICROBIAL BIOMASS - The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.05-31-2012
20120135478ALGAL MEDIUM CHAIN LENGTH FATTY ACIDS AND HYDROCARBONS - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a 05-31-2012
20120077235CHAIN-SELECTIVE SYNTHESIS OF FUEL COMPONENTS AND CHEMICAL FEEDSTOCKS - A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives. A method for producing cycloalkanes for jet fuel by providing a starting composition comprising at least one selected from the group consisting of algal and polyunsaturated vegetable oils, subjecting the starting composition to metathesis to produce metathesis product comprising at least one olefin, cyclohexadiene, and at least one acid-, ester-, or salt-functionalized alkene, and reacting the at least one olefin and cyclohexadiene to form cycloalkane(s).03-29-2012
20120077234METHOD AND SYSTEM FOR MICROBIAL CONVERSION OF CELLULOSE TO FUEL - A method and system are provided for producing biofuel from cellulosic feedstock. In the method, the cellulosic feedstock is pretreated to separate cellulose, hemicellulose, and lignin. Thereafter, the cellulose and hemicellulose are converted into sugars through enzymatic hydrolysis. Then, the sugars are converted into lipids, e.g. triglycerides, through a microbial process. Specifically, heterotrophic microalgae is grown on the triglycerides and forms triglycerides. While triglycerides are formed from the cellulose and hemicellulose, the lignin is converted into ringed hydrocarbons, such as aromatic hydrocarbons and cycloalkanes, e.g., cycloparaffins. To form the biofuel, the triglycerides and ringed hydrocarbons are processed together. During this step, the triglycerides are converted into straight chain paraffins and esters. Preferably, the biofuel is a surrogate for jet quality JP-8 fuel.03-29-2012
20100047884Method for Producing Biodiesel - The invention relates to the utilisation of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C02-25-2010
20120178134ENHANCEMENT OF BIOMASS PRODUCTION BY DISRUPTION OF LIGHT ENERGY DISSIPATION PATHWAYS - The invention provides a method of producing biomass or at least one biomolecule comprising culturing a photosynthetic microorganism that comprises a disrupted Non-Photochemical Quenching (NPQ) process, and isolating biomass or at least one biomolecule from the culture.07-12-2012
20100021976PRODUCTION OF POLYUNSATURATED FATTY ACIDS, NOVEL BIOSYNTHESIS GENES, AND NOVEL PLANT EXPRESSION CONSTRUCTS - The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11 encoding polypeptides having desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C01-28-2010
20120190088METHOD OF PRODUCING FATTY ACID ALKYL ESTER USING MICROORGANISMS HAVING ABILITY TO PRODUCE OIL - The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.07-26-2012
20120329109Genetically Engineered Microorganisms That Metabolize Xylose - The invention provides methods of cultivating oil-bearing microbes using xylose as a fixed carbon source. Also provided are microorganisms that have been genetically engineered to metabolize xylose as a fixed carbon source allowing them to convert xylose into oils. Particular advantages of the processes provided herein include production of oils rather than alcohols through the microbial fermentation processes utilizing xylose.12-27-2012
20120231513SYSTEMS AND METHODS FOR CULTURING ALGAE WITH BIVALVES - Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.09-13-2012
20110003350SYSTEM AND METHOD FOR HIGH-VOLTAGE PULSE ASSISTED AGGREGATION OF ALGAE - A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.01-06-2011
20120322116METHOD FOR TRANSFORMING STRAMENOPILES - Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class 12-20-2012
20110045556ALGAL LIPID HARVEST USING MOLLUSKS FOR BIOFUELS PRODUCTION - Embodiments of the present disclosure provide for novel strategies to harvest algal lipids using mollusks which after feeding algae from the growth medium can convert algal lipids into their biomass or excrete lipids in their pseudofeces which makes algae harvesting energy efficient and cost effective. The bioconverter, filter-feeding mollusks and their pseudofeces can be harvested and converted to biocrude using an advanced thermochemical liquefaction technology. Methods, systems, and materials are disclosed for the harvest and isolation of algal lipids from the mollusks, molluscan feces and molluscan pseudofeces.02-24-2011
20110045555ENZYMATIC MODIFICATION OF OIL - The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.02-24-2011
20110045554HETEROLOGOUS EXPRESSION OF EXTREMOPHILE HEAT SHOCK PROTEINS AND CHAPERONES IN MICROORGANISMS TO INCREASE TOLERANCE TO TOXIC COMPOUNDS - The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.02-24-2011
20120270283ENZYME INTERESTERIFICATION PROCESS - A fatty material enzyme interesterification process comprising the steps of: a) providing a fatty material that has optionally undergone at least one prior purification treatment; b) introducing soap into said fatty material to form a soap-containing fatty material mixture, c) homogenising said soap-containing fatty material mixture by mixing, d) contacting said soap-containing fatty material mixture with lipase to produce a soap-containing interesterified fatty material, e) removing soaps from said soap-containing interesterified fatty material to yield interesterified fatty material, wherein said soap is either formed in-situ by addition of aqueous alkali to said fatty material or said soap is introduced directly into said fatty material.10-25-2012
20120270284Method And System For Robotic Algae Harvest - A Robotic Algae Harvester (RAH) of the present invention works by providing a CO10-25-2012
20100233767PROCESS FOR THE RECOVERY OF MAGNESIUM FROM A SOLUTION AND PRETREATMENT - A process for the recovery of magnesium from a solution containing soluble magnesium, the process comprising, precipitating magnesium hydroxide from the solution, forming an oxide blend including magnesium oxide derived from the precipitated magnesium hydroxide together with calcium oxide, reducing the oxide blend to form a magnesium metal vapour and condensing the vapour to recover magnesium metal.09-16-2010
20110281312HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM FOR BIOCATALYTIC SYNTHESIS OF A STRUCTURED LIPID - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.11-17-2011
20120100581PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.04-26-2012
20120288906Process For Obtaining Fatty Acid Lower Alkyl Esters From Unrefined Fats And Oils - Described is a process for obtaining fatty acid C11-15-2012
20100184168PROCESS FOR PRODUCING FATTY ACIDS - A process for producing fatty acid by hydrolyzing oil and fat, which comprises: a first step of partially hydrolyzing oil and fat by either of the following method (a) or (b), and a second step of hydrolysis by the other method: (a) enzymatic hydrolysis using an immobilized enzyme which is an enzyme immobilized on a support and (b) high pressure and temperature hydrolysis. A method of efficiently producing fatty acid with a reduced content of trans-unsaturated fatty acid in the constituent fatty acids and having a good appearance with reduced coloring by hydrolysis of oil and fat is provided.07-22-2010
20100167359METHODS FOR PRODUCING POLYUNSATURATED FATTY ACID AND LIPID CONTAINING POLYUNSATURATED FATTY ACID - A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.07-01-2010
20130011889GENERATION OF TRIACYLGLYCEROLS - A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which results in the formation of lyso-phospholipids and free fatty acids. The diacylglycerols and the free fatty acids from these two separate reactions then combine in the presence of the enzymes to generate new triacylglycerol molecules.01-10-2013
20100129882HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.05-27-2010
20130171702METHOD FOR CULTURING MIXOTROPHIC UNICELLULAR ALGAE IN THE PRESENCE OF A DISCONTINUOUS SUPPLY OF LIGHT IN THE FORM OF FLASHES - A novel method for culturing mixotrophic single-cell algae makes it possible to enrich the lipid content of these algae, the enrichment being induced by a variable or discontinuous provision of light, in particular in the form of flashes.07-04-2013
20080241897Enzymatic Oil Interesterification - An improved process for enzymatic interesterification of oil containing a chelating agent with a base and a lipase.10-02-2008
20080227165Process for producing conjugated fatty acid and food/drink produced by the process - A conjugated fatty acid such as the cis-9, trans-11 isomer of conjugated linoleic acid having high bioactivities is selectively and efficiently produced by conjugating an unsaturated fatty acid having at least two double bonds with the use of viral cells, dead cells or a cell extract of at least one bacterium selected from the group consisting of 09-18-2008
20080220486Method for growing photosynthetic organisms - A method of growing photosynthetic organisms comprising providing the organisms with flue gases from a fossil-fuel power plant, the gases being previously treated by desulfurization. The carbon dioxide (CO09-11-2008
20130143281Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from 06-06-2013
20130143282ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL PRODUCTION - Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.06-06-2013
20130143283Integrated Bio-Digestion Facility - Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester.06-06-2013
20130143284MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.06-06-2013
20120252080METABOLIC PATHWAY TARGETING BY TRANSCRIPTION FACTOR OVEREXPRESSION - The invention provides microorganisms such as cyanobacteria genetically engineered to express proteins that include transcription factor domains for upregulation of lipid biosynthetic pathways. In addition to expression a gene encoding a transcription factor domain protein, the recombinant microorganisms can express at least one exogenous gene that encodes a polypeptide for the production of a fatty acid, fatty acid derivative, or triglyceride. Also included are methods of producing a fatty acid, fatty acid derivative, or triglyceride using the engineered microorganisms described herein as well as nucleic acid molecules encoding novel transcription factor domain proteins.10-04-2012
20120252079YARROWIA DIACYLGLYCEROL ACYLTRANSFERASE PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST - Promoter regions associated with the 10-04-2012
20130115665METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS - Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.05-09-2013
20130115664PROCESS AND SYSTEM FOR PRODUCING ALGAL OIL - A method for producing an algal oil is provided. The method includes continuously providing a growth medium and an algal strain to a bioreactor at a predetermined fluid flow rate; illuminating the growth medium and algal strain contained within the bioreactor by a first artificial light source for a time sufficient to effect lipid production by the algal strain; continuously withdrawing a portion of the growth medium and algal strain contained within the bioreactor at the predetermined fluid flow rate; and treating the withdrawn portion of the growth medium and algal strain to produce and isolate a lipid produced by the algal strain.05-09-2013
20130095537PROCESS FOR PRODUCTION OF USEFUL SUBSTANCES - A process for producing a useful substance using microorganisms, and an object thereof to provide a method that shortens the culturing period of the microorganisms and improves the productivity of the useful substance. The speed at which microorganisms consume carbohydrates and/or hydrocarbon oxidation products can be increased and the productivity of a target useful substance can be improved by using a main culture initial medium in which the total concentration of carbohydrate and hydrocarbon oxidation product is less than 0.4% by weight in terms of the carbon equivalent.04-18-2013
20130095536CARBON DIOXIDE CAPTURE BOX - The present subject matter relates to a system comprising a first unit that generates a polluted air comprising carbon dioxide, and a second unit mounted on the first unit. The second unit comprises a solution comprising at least one alga suspended in a solvent to purify the polluted air. The solution comprising the at least one alga is agitated upon operation of the first unit. The system further comprises a channel to guide the polluted air from the first unit to the second unit.04-18-2013
20130115666METHOD FOR PRODUCTION OF EUGLENA CONTAINING WAX ESTER AT HIGH COTENT, AND METHOD FOR PRODUCTION OF WAX ESTER - Provided is a method for producing a 05-09-2013
20130102041ENZYMATIC PROCESS FOR SYNTHESIZING ESTOLIDES - This invention is a process for enzymatic synthesis of estolides through the reaction between stearic acid and methyl ricinoleate, using an immobilized lipase as the catalyst, in a solvent-free medium. This process prevents degradation of the product, reduces the formation of secondary products that generate color and odor in the lubricant, and allows recovery of the lipase and its subsequent reuse.04-25-2013
20130102040USE OF ENDOGENOUS PROMOTERS IN GENETIC ENGINEERING OF NANNOCHLOROPSIS GADITANA - The present disclosure is directed to novel polynucleotide sequences for use in 04-25-2013
20130102039Methods of Producing Triacylglyceride Compositions Comprising Tailored Oils - Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.04-25-2013
20130130336CHAIN-SELECTIVE SYNTHESIS OF FUEL COMPONENTS AND CHEMICAL FEEDSTOCKS - A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives. A method for producing cycloalkanes for jet fuel by providing a starting composition comprising at least one selected from the group consisting of algal and polyunsaturated vegetable oils, subjecting the starting composition to metathesis to produce metathesis product comprising at least one olefin, cyclohexadiene, and at least one acid-, ester-, or salt-functionalized alkene, and reacting the at least one olefin and cyclohexadiene to form cycloalkane(s).05-23-2013
20130130334BIOFUEL AND ELECTRICITY PRODUCING FUEL CELLS AND SYSTEMS AND METHODS RELATED TO SAME - A fuel cell comprising an anode electrode, a cathode electrode and a reference electrode electronically connected to each other; a first biocatalyst comprising a consolidated bioprocessing organism (e.g., a cellulomonad or 05-23-2013
20130102042GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION - The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant 04-25-2013
20120276602Method For Treatment of Oil - The present invention relates to a method for treatment of oil with a lipolytic enzyme which comprises contacting of the oil with particles of base-containing porous amorphous silica.11-01-2012
20130149753Application of Bacteriophages for the Control of Unwanted Bacteria in Biofuel Production Mediated by Non-Bacterial Reactive Agents - A method of reducing process interruptions in biofuel production systems by reducing the amount of unwanted bacteria in the biofuel production system in which the reduction is effected by the use of an effective amount of one or more types of bacteriophages virulent for at least some strains of the unwanted bacteria.06-13-2013
20130149754Optimization of Lipid Synthesis and Accretion - The present invention relates to a novel mutant strain of yeast, particularly a strain of 06-13-2013
20120258505HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.10-11-2012
20100297714Multi-Step Method for Producing Algae Products - A multi-step method for producing an algae product comprising, a microorganism consumption step, another step, and an algae product collection step. The microorganism consumption step comprises, combining a liquid growth medium comprising microorganisms with a phagotrophic algae capable of producing a desired algae product, consuming said microorganisms by said phagotrophic algae, and growing said phagotrophic algae. Another step comprises either a microorganism growth step or a photosynthetic algal growth step. A microorganism growth step comprises providing a liquid growth medium comprising nutrients and microorganisms capable of said consuming said nutrients, consuming said nutrients by said microorganisms, and growing said microorganisms. A photosynthetic algal growth step comprises providing a substantially organic nutrient depleted liquid medium, providing a microorganism population comprising said phagotrophic algae, photosynthetic growth of said phagotrophic algae. An algae product collection step comprises collecting a desired algae product from said phagotrophic algae.11-25-2010
20100317072OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION - Described are engineered strains of the oleaginous yeast 12-16-2010
20100317071Method for the Enzymatic Production of Fatty Alcohol and/or Fatty Acid - The invention relates to a method for the enzymatic production of C12-16-2010
20130157325Lipolytic Enzyme Variants - Molecular dynamics (MD) simulation on the three-dimensional structure of 06-20-2013
20130157324IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.06-20-2013
20120282662METHODS OF AND SYSTEMS FOR PRODUCING DIESEL BLEND STOCKS - A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.11-08-2012
20110306100METHOD OF PRODUCING FATTY ACIDS FOR BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALSPCT/ - The present invention relates to a method of producing fatty acids, by (i) inoculating a mixture of at least one of cellulose, hemicellulose, and lignin with at least one microorganism strain that produces one or more cellulases, hemicellulases and laccase, that hydrolyze at least one of cellulose, hemicellulose and lignin, under conditions to produce at least one of glucose, cellobiose, xylose, mannose, galactose, rhamnose, arabinose or other hemicellulose sugars; (ii) inhibiting growth of the at least one microorganism strain; (iii) inoculating the mixture of step (ii) with at least one algae strain that metabolizes the at least one of glucose, cellobiose, xylose, mannose, galactose, rhamnose, arabinose or other hemicellulose sugars, under conditions so that the at least one algae strain produces one or more fatty acids; and optionally (iv) recovering the one or more fatty acids from the at least one algae strain.12-15-2011
20110312045FERMENTATION PROCESSES - The present invention provides a method for the production of at least one of a fermentation product and a derivate thereof comprising the steps of: a. providing a fermentable aqueous solution comprising at least one fermentable compound and at least one impurity; b. selectively extracting the at least one impurity from at least a portion of the fermentable aqueous solution by means of an extractant to form an impurity-depleted fermentable solution comprising a fermentable compound and to form an impurity-comprising extract; and c. fermenting the fermentable compound of the impurity-depleted fermentable solution to form a fermentation broth comprising a fermentation product wherein the extractant comprises at least one of the fermentation product and a derivate thereof.12-22-2011
20130189749Compositions, Methods, and Kits for Polyunsaturated Fatty Acids from Microalgae - The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus 07-25-2013
20120021470Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from 01-26-2012
20120028319CHLORELLA SPECIES CONTAINING EXOGENOUS GENES - The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.02-02-2012
20120028318FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA - Described are compositions and methods relating to a fungal cutinase cloned from 02-02-2012
20130196391PRODUCTION OF FATTY ACIDS BY HETEROLOGOUS EXPRESSION OF GENE CLUSTERS FROM MYXOBACTERIA - The invention relates to a process for producing one or more polyunsaturated fatty acids by means of heterologous gene expression comprising the steps of providing a production organism which comprises a heterologous gene cluster encoding a polyunsaturated fatty acid biosynthetic pathway encompassing a subsequence ER encoding an enoylreductase and a subsequence AT encoding an acyltransferase, growing the production organism in the presence of a fermentable carbon source whereby one or more polyunsaturated fatty acids are produced, and optionally recovering the one or more polyunsaturated fatty acids.08-01-2013
20130196390METHOD OF PRODUCING BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALS - a method for producing a hydrocarbon based product (e.g., biofuel or biodiesel), a microorganism (e.g., algae) that can be cultivated and harvested for producing a hydrocarbon based product, a method for producing a hydrocarbon based product from a by-product of biodiesel production, and a microorganism that can be cultivated from a by-product of biodiesel production and harvested for producing hydrocarbon based product.08-01-2013
20130196392MICROALGAE BIOFUEL PRODUCTION SYSTEM - A system for processing oil from algae is disclosed. Specifically, the system involves the use of a consortium of algae strains as its input, wherein each algae strain has a unique characteristic for resisting/dominating a particular operational/environmental factor. Also, the system recycles byproducts of the process for use as nutrients during algae growth and oil production. The system includes a conduit for growing algae and an algae separator that removes the algae from the conduit. Also, the system includes a device for lysing the algae and an oil separator to remove the oil from the lysed matter. Further, the system includes a biofuel reactor that receives oil from the oil separator and synthesizes biofuel and glycerin. Moreover, the algae separator, oil separator and biofuel reactor all recycle byproducts back to the conduit to support further algae growth.08-01-2013
20130196393PROCESS FOR SEPARATING POLYUNSATURATED FATTY ACIDS FROM LONG CHAIN UNSATURATED OR LESS SATURATED FATTY ACIDS - A process for separating polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapen-taenoic acid (EPA) from less saturated long chain fatty acids (LCFAs) in a lipid composition, wherein said PUFAs and LCFAs are present as (i) triglycerides, or (ii) free fatty acids or monoalkyl esters, by exchange of at least a portion of LCFAs with short and/or medium chain fatty acids (MCFAs). The process can suitably be employed on marine derived oil, marine oil 2 derived oil products and other sources of PUFAs, including PUFA-rich singe cell oils (SCOs), and oils from genetically modified organisms with a modified lipid metabolism. The inventive process is based on novel use of lipases and distillation techniques, selectively chemically modifying species in the substrate material such that the desired species and chemically similar species become sufficiently dissimilar to be separable. Thus PUFA can be effectively enriched from material such as 30 herring oil with low PUFA content and high contant of equal length monounsaturated fatty acids such as 20:1 and 22:1 fatty acids.08-01-2013
20130196394COMPOSITIONS AND METHODS FOR BACTERIAL LYSIS AND NEUTRAL LIPID PRODUCTION - A cyanobactenum that produces neutral lipids or alkanes, such as FAME OR FAEE, which may be used for biofuel production.08-01-2013
20120040420Flavor Compound-Producing Yeast Strains - Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.02-16-2012
20130203133BUOYANT TRIACYLGLYCEROL-FILLED GREEN ALGAE AND METHODS THEREFOR - Cultures of 08-08-2013
20120070868PRODUCTION OF ODD CHAIN FATTY ACID DERIVATIVES IN RECOMBINANT MICROBIAL CELLS - Recombinant microbial cells are provided which have been engineered to produce fatty acid derivatives having linear chains containing an odd number of carbon atoms by the fatty acid biosynthetic pathway. Also provided are methods of making odd chain fatty acid derivatives using the recombinant microbial cells, and compositions comprising odd chain fatty acid derivatives produced by such methods.03-22-2012
20120088279ALGAL LIPID PRODUCTION - Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described.04-12-2012
20120094340Production of Ester-Based Fuels Such As Biodiesel From Renewable Starting Materials - Production of ester-based fuels such as biodiesel or jet fuel from renewable starting materials such as lignocellulosic material or algae is disclosed. Pulping and saccharification of the renewable starting materials produces carboxylic acids such as fatty acids or rosin acids, which are esterified via a gas sparged, slurry form of heterogeneous reactive distillation to yield ester-based fuels.04-19-2012
20120094339MICRO-ALGA BELONGING TO GENUS NAVICULA, PROCESS FOR PRODUCTION OF OIL BY CULTURE OF THE MICRO-ALGA, AND OIL COLLECTED FROM THE MICRO-ALGA - Disclosed are: a microalga highly capable of producing aliphatic hydrocarbons of 16 to 26 carbon atoms; a process for producing oil, which comprises a step of culturing the microalga; oil collected from the microalga; a fuel produced from the microalga; and a method for fixing carbon dioxide, which comprises a step of culturing the microalga.04-19-2012
20120094338CONTINUOUS PRODUCTION AND EXCRETION OF WAXY PRODUCTS FROM PHOTOSYNTHETIC ORGANISMS - This invention disclosed herein relates generally to production and maintenance of genetically modified organisms that are capable of continuously making and excreting valuable waxy products.04-19-2012
20130210093METHODS FOR PRODUCTION OF ALGAE DERIVED OILS - The present invention comprises a method of producing algae derived oils comprising growing, stressing, and harvesting algae and then extracting total lipids from which specific oil fractions are purified.08-15-2013
20130210094HETEROGENEOUS ENZYMATIC CATALYST, PROCESS FOR PREPARING SAME AND USE FOR CONTINUOUS FLOW ENZYMATIC CATALYSIS - The present invention relates to a heterogeneous enzymatic catalyst consisting of a macroprous silica monolith incorporating an enzyme immobilized by means of a compiling agent, to a process for preparing this enzymatic catalyst, to the use of the catalyst for carrying out chemical reactions by continuous flow heterogeneous enzymatic catalyst and to a process of continuous flow heterogeneous enzymatic catalysis using said catalyst.08-15-2013
20130210095NOVEL STRAINS OF MICROALGAE OF THE GENUS BOTRYOCOCCUS AND METHOD FOR THE CULTURE OF SAID MICROALGAE IN MIXOTROPHIC MODE - Novel strains of microalgae which belong to the 08-15-2013

Patent applications in class Fat; fatty oil; ester-type wax; higher fatty acid (i.e., having at least seven carbon atoms in an unbroken chain bound to a carboxyl group); oxidized oil or fat