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Preparing compound containing saccharide radical

Subclass of:

435 - Chemistry: molecular biology and microbiology

435041000 - MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435084000 Preparing nitrogen-containing saccharide 842
435099000 Produced by the action of a carbohydrase (e.g., maltose by the action of alpha amylase on starch, etc.) 152
435101000 Polysaccharide of more than five saccharide radicals attached to each other by glycosidic bonds 81
435105000 Monosaccharide 72
435074000 Preparing O-glycoside (e.g., glucosides, etc.) 40
435100000 Disaccharide 22
435096000 Produced by the action of an exo-1.4 alpha glucosidase (e.g., dextrose by the action of glucoamylase on starch, etc.) 18
435094000 Produced by the action of an isomerase (e.g., fructose by the action of xylose isomerase on glucose, etc.) 18
435097000 Produced by the action of a glycosyl transferase (e.g., alpha, beta, gamma-cyclodextrins by the action of glycosyl transferase on starch, etc.) 17
435095000 Produced by the action of a beta-amylase (e.g., maltose by the action of beta-amylase on amylose, etc.) 9
435093000 Mashing or wort making 5
20090269818Mashing Process - The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process.10-29-2009
20090269817PRETREATMENT OF GRAIN SLURRY WITH ALPHA-AMYLASE AND A HEMICELLULASE BLEND PRIOR TO LIQUEFACTION - A method of preparing a low viscosity slurry that includes grinding a small grain to produce a flour. The flour is mixed with water to form a slurry. An alpha-amylase enzyme and a hemicellulase blend enzyme are mixed into the slurry and allowed to convert the slurry into a mash. A saccharifying enzyme is mixed into the mash. It is possible to use coarse grains such as grain sorghum and maize in conjunction with the small grains.10-29-2009
20120288899CONTINUOUS METHOD OF PRODUCING A MASH EXTRACT - The present invention relates to a mash extract and a continuous method of producing the mash extract by decoction mashing. The method comprises: (a) mixing a first malt enzyme source with an aqueous liquid to obtain an aqueous malt enzyme suspension; (b) separately, mixing a second enzyme source with one or more starch-containing adjuncts to obtain a decoction suspension; (c) subjecting the decoction suspension to a first heat treatment at 60-85° C. and then a second heat treatment at a higher temperature; (d) combining the heated decoction suspension from the second heat treatment with the aqueous malt enzyme suspension to obtain a mash; (e) maintaining the mash at 35-85° C. for a time; and (f) removing spent grain from the heated mash to produce a mash extract.11-15-2012
20100035304METHOD OF PRODUCING A MASH EXTRACT AND AN APPARATUS FOR CARRYING OUT SUCH METHOD - One aspect of the invention concerns a method comprising: 02-11-2010
20120077232SYSTEM AND METHOD FOR CONTROLLING A FERMENTATION PROCESS - A method for controlling a fermentation process includes injecting a mash into a fermenter and injecting a liquid yeast additive into the fermenter. The liquid yeast additive is injected in a closed-loop manner. The method may be used to control the fermentation processes of one or more fermenters operating in parallel.03-29-2012
435098000 Produced by the action of an alpha-1, 6-glucosidase (e.g., amylose debranched amylopectin by the action of pullulanase, etc.) 4
20130089897Polypeptides Having Isoamylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having isoamylase activity derived from 04-11-2013
20120301927Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant - Variants of a 11-29-2012
20120003700Thermostable Alpha-Amylases - The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: 01-05-2012
20080220482Production of Crystalline Short Chain Amylose - A process for producing a starch comprises treating a feed starch that comprises amylopectin with glucanotransferase to produce a chain-extended starch, and treating the chain-extended starch with a debranching enzyme to produce a starch product that comprises amylose fragments. At least about 38% by weight of the amylose fragments have a degree of polymerization (DP) of at least about 35.09-11-2008
435073000 Preparing S-glycoside (e.g., lincomycin, etc.) 1
20110136177Method of Producing a Low Molecular Weight Organic Compound in a Cell - A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.06-09-2011
Entries
DocumentTitleDate
20110177561Novel CBH1 Homologs And Varian CBH1 Cellulase - Disclosed are a number of homologs and variants of 07-21-2011
20110177560Cellulosic Protein Expression in Yeast - The present invention provides for combinations of enzymes and other proteins that result in improved saccharification of plant material. The invention provides for saccharification in the presence of and optional fermentation by, yeast cells expressing the enzymes and other proteins.07-21-2011
20100120095ELECTROMAGNETIC BIOACCELERATOR - The present invention relates to an electromagnetic bioaccelerator for obtaining biomass by simulating environmental marine conditions, comprising at least the following elements: octagonal biomass converters (05-13-2010
20100120096METHOD FOR PRODUCING LACTO-N-BIOSE I AND GALACTO-N-BIOSE - A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided.05-13-2010
20100075381Method for Producing Glucuronic Acid by Glucuronic Acid Fermentation - The object of the present invention is to provide a microorganism having an excellent ability to specifically oxidize the hydroxymethyl group of glucose, and a method of producing glucuronic acid and/or glucuronolactone by using such a microorganism to directly oxidize glucose, and the invention is directed at a microorganism which produces glucuronic acid directly from glucose and a mutant strain which is capable of specifically oxidizing the hydroxymethyl group of glucose and in which a DNA nucleotide sequence corresponding to 16S rRNA has the nucleotide sequence of SEQ ID NO: 1 in the sequence listing, and the invention is also directed at a method of producing glucuronic acid and/or glucuronolactone by specifically oxidizing the hydroxymethyl group of glucose using the mutant strain, and this invention enables D-glucuronic acid and/or D-glucuronolactone to be produced and furnished easily and safely at a high yield and a low cost.03-25-2010
20130040342Thermostable Phytase Variants - The present invention relates to a method for producing phytase variants has at least 70% identity to a phytase derived from 02-14-2013
20100041104Compositions and Methods Comprising Cellulase Variants with Reduced Affinity to Non-Cellulosic Materials - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.02-18-2010
20130137142Expression of Beta-Mannanase in Chloroplasts and its Utilization in Lignocellulosic Woody Biomass Hydrolysis - Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. In other exemplary embodiments, methods and materials are disclosed for degrading mannans.05-30-2013
20090305355Method for Syngas-Production from Liquefied Biomass - The present invention relates to methods for syngas-production from biomass enabling the conversion of pre-treated biomasses having a high dry-matter content into electricity or oil-based products such as petrol, diesel, chemicals and plastics through the formation of syngas. The biomasses are converted into a biomass slurry having a suitable particle size and dry-matter content for optimal feeding and gasification in a pressurised gasifier.12-10-2009
20090305356METHODS AND APPARATUS FOR THE USE OF ULTRASONIC ENERGY TO IMPROVE ENZYMATIC ACTIVITY DURING CONTINUOUS PROCESSING - Described herein are methods and devices for increasing enzymatic activity during continuous processing by applying ultrasonic energy.12-10-2009
20090233335Variant humicola Grisea CBH1.1 - Disclosed are variants of 09-17-2009
20120237980CONTINUOUS COUNTER-CURRENT ORGANOSOLV PROCESSING OF LIGNOCELLULOSIC FEEDSTOCKS - A modular process for organosolv fractionation of lignocellulosic feedstocks into component parts and further processing of said component parts into one or more of a de-lignified cellulose stream, a sugar stream, small-chain alcohol streams and four structurally distinct classes of lignin derivatives. The modular process comprises a first processing module configured for digesting lignocellulosic feedstocks with an organic solvent thereby producing a cellulosic solids fraction and a liquid fraction, a second processing module configured for recovering small-chain alcohols and optionally a first class of lignin derivatives from the cellulosic solids fraction, a third processing module configured for recovering from the liquid fraction at least one of a second class and a third class of lignin derivatives or mixtures thereof, and waste stream comprising a fourth class of lignin derivatives. The fourth processing module may optionally recover the fourth class of lignin derivatives.09-20-2012
20110033897Method for the Obtainment of Hydrolytic Enzymes, Hydrolytic Method for Producing Fermentable Sugars, Additives comprising Fermentable Sugars, and Process for Producing Ethanol from Sugar Cane Bagasse - The present invention provides industrial methods involving the hydrolysis of sugar cane bagasse, including methods for producing hydrolytic enzymes, for hydrolysis of sugar cane bagasse, for the production of additives comprising fermentable sugars and the respective additives, and methods of producing ethanol. The invention methods comprised the submerged culture of 02-10-2011
20110281303POLYPEPTIDES HAVING ACETYL XYLAN ESTERASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having acetyl xylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.11-17-2011
20110136175Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from 06-09-2011
20110143398Methods of saccharification of polysaccharides in plants - Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional β-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.06-16-2011
20110294164VARIANT HUMICOLA GRISEA CBH1.1 - Disclosed are variants of 12-01-2011
20110294166THERMOSTABLE ENZYMES FOR THE HYDROLYSIS OF MANNAN-CONTAINING POLYSACCHARIDES - The present disclosure relates to hydrolysis of mannan-containing poly- or oligo-saccharides by use of a polypeptide having endo-β-mannanase activity. In particular the present disclosure relates to compositions comprising a bacterial endo-β-mannanase, polynucleotides encoding the endo-β-mannanase, and methods of use thereof.12-01-2011
20110294163Of Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Agricultural Residues - A method for producing a fermentation product from a lignocellulose-containing material comprises pre-treating the lignocellulose-containing material; introducing agricultural residues to the pre-treated lignocellulose-containing material; exposing the pre-treated lignocellulose-containing material to one or more hydrolyzing enzymes; and fermenting with a fermenting organism to produce a fermentation product. The agricultural residues may be corn pith and/or corn cob.12-01-2011
20110269189Method for post-extracting high-acyl gellan gum - Disclosed is a method for extracting high acyl gellan from the fermentation broth containing gellan gum with a low production cost and high quality of products, comprising the following steps: (1) Treatment of the fermentation broth with an enzyme; (2) flocculation of the treated fermentation broth with acid; (3) wash of the fiber-like material; and (4) drying and milling.11-03-2011
20120190071LIPA AND ITS VARIANT USEFUL FOR BIOFUEL PRODUCTION - The present invention relates to LipA and its variant that binds to the glucosyl- and acyl chain- region of its glycoside substrate in plant cell walls useful for biofuel production, wherein presence of a hydrophobic pocket confers extensive hydrophobic interaction of the acyl chain with the rest of the tunnel residues.07-26-2012
20100035302METHOD FOR MAKING SOLUBLE ARABINOXYLANS AS CO-PRODUCT OF FERMENTATION OF WHOLE-GRAIN CEREALS - The present invention provides a method for producing preparations comprising soluble arabinoxylans as co-products of ethanol production through fermentation of whole-grain cereals. The method results in preparations that are highly enriched in soluble arabinoxylans, which can be used as a food, beverage, or feed ingredients.02-11-2010
20090311752Conditioning Biomass for Microbial Growth - The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.12-17-2009
20100267090NOVEL SACCHARIDE PRIMER - The present invention discloses a saccharide primer for synthesizing, in culture cells, an O-glycan sugar chain having the structure of sugar chain-amino acid-alkyl group or alkyl group derivative.10-21-2010
20100267089ENDOGLUCANASES - The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.10-21-2010
20120270270VARIANT HUMICOLA GRISEA CBH1.1 - Disclosed are variants of 10-25-2012
20090117619CARBOHYDRASE EXPRESSION DURING DEGRADATION OF WHOLE PLANT MATERIAL BY SACCHAROPHAGUS DEGRADANS - The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose, hemicellulose and other carbohydrates. These systems have a number of applications.05-07-2009
20100143974Process for Sugar Production from Lignocellulosic Biomass Using Alkali Pretreatment - We have discovered a new method to treat biomass with alkali, for example lime. The lime and lignin was sufficiently removed from the treated biomass b> squeezing with a high pressure device to remove alkali and other potential inhibitors of the cellulase enzymes added for sacchaπfication. The resulting fibrous material was rapidly solubilzed by cellulases, even at solid loads ranging from 10 to 30% (w/w) without inhibitory effects on the cellulase activity. The lime pretreatment removed lignin effectively and left the cellulose and hemicellulose almost intact. The method yielded a biomass with structure capable of being enzyme solubilzed and fermented readily at a solids loading of 10-30% for a production of ethanol.06-10-2010
20080241887Polypeptides With Starch Debranching Activity - Polypeptides comprising a catalytic starch debranching domain and a starch binding domain are disclosed. The polypeptides comprising the starch binding domain have an improved functionality in raw starch degradation compared with same catalytic unit in same amount but without the starch binding domain.10-02-2008
20080280328Glucoamylase Variants - The present invention relates to glucoamylase variants with improved properties and methods of utilizing the glucoamylase variants.11-13-2008
20110207177SUGAR PRODUCTION PROCESS AND ETHANOL PRODUCTION PROCESS - The present invention provides a pretreatment method which enables the promotion of the enzymatic glycosylation of lignocellulose under relatively mild conditions by using a tree bark as a raw material with less energy. Specifically the present invention provides a step for producing a sugar from a tree bark, which is characterized by having the following steps: an alkali treatment step of immersing the tree bark in an alkali compound solution; a refining treatment step of refining the alkali treated tree bark mechanically into fine pieces; and an enzymatic glycosylation step of glycosylating the refined tree bark with an enzyme. The present invention also provides a method of producing an ethanol.08-25-2011
20110207176METHOD AND APPARATUS FOR PRODUCING ALCOHOL OR SUGAR USING A COMMERCIAL-SCALE BIOREACTOR - Provided is a method of producing alcohol or sugar in a commercial-scale bioreactor using a reformulated commercial enzyme preparation. Also provided is a bioreactor modified to practice the method.08-25-2011
20090186383Method of Extracting Starches and Sugar from Biological Material Using Controlled Cavitation - A method of extracting sugar, starch, and/or carbohydrates from feed material such as corn or corn stover is disclosed. The feed material is mixed with liquid and perhaps accelerants to form a mixture. The mixture is pumped through a controlled cavitation reactor, where it is exposed to shockwaves from cavitation events. The shockwaves open pores in the feed material and force liquid in and out of the pores to liberate trapped sugars and starches, which are dissolved in the liquid for subsequent removal.07-23-2009
20130217070PRODUCTION OF XYLITOL FROM A MIXTURE OF HEMICELLULOSIC SUGARS - Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.08-22-2013
20090081734Arabinitol Dehydrogenases from Neurospora crassa - Stable and active arabinitol dehydrogenases (LAD) from 03-26-2009
20090004697Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization - The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.01-01-2009
20110229936Polypeptides Having Beta-Glucosidase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.09-22-2011
20090275085 Enzyme for Obtaining Prebiotic Oligosaccharides - An industrially viable process of obtaining prebiotic oligosaccharides using a new 11-05-2009
20110177559PROCESSING MATERIALS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a liquid medium and a saccharifying agent.07-21-2011
20110177558DISPERSING FEEDSTOCKS AND PROCESSING MATERIALS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified.07-21-2011
20100184151METHOD FOR THE PRODUCTION OF GLUCOSE FROM LIGNOCELLULOSIC FEEDSTOCKS - A method for producing glucose from a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock with acid to produce a pretreated feedstock composition. A calcium-containing stream is provided that comprises calcium that is obtained from the lignocellulosic feedstock and a calcium carbonate-containing stream is obtained by precipitation of the calcium from the calcium-containing stream. The pH of the pretreated feedstock is adjusted with (a) the calcium carbonate-containing stream; (b) a calcium hydroxide-containing stream that is derived from said calcium carbonate-containing stream by subjecting said calcium carbonate-containing stream to calcination; or (c) a combination of the calcium carbonate-containing stream and the calcium hydroxide-containing stream. The pH adjustment results in a neutralized pretreated lignocellulosic feedstock having a pH between about 3 and about 9 and enzymatic hydrolysis of the neutralized, pretreated lignocellulosic feedstock is then conducted with cellulase enzymes to produce the glucose.07-22-2010
20100151526Method of Producing Sucrose-6-Acetate by Whole-Cell Biocatalysis - A process is described which uses whole cell preparations, immobilized or without immobilization, of a microorganism, including 06-17-2010
20090137006Sugar Kinases with Expanded Substrate Specificity and Their Use - One preferred embodiment of the present invention provides a GalK variant comprising a Y371H, M173L or Y371H-M173L mutation for in vivo and in vitro glycorandomization. In another preferred embodiment, the 05-28-2009
20090053771Process for making fuels and chemicals from AFEX-treated whole grain or whole plants - A process for hydrolyzing whole grain or whole plant biomass after an Ammonia Fiber Explosion (AFEX) process step is described. The process preferably uses a biomass that is hydrolyzed using a different combination of enzymes (amylase, cellulase and hemicellulase) to sugars for fermentation to produce ethanol. Harvesting the whole plant inclusive of grains and stalk for ethanol bio-processing is an economical route for future biorefineries. In addition to sugars, various value-added products like proteins and oil can be co-generated.02-26-2009
20100255542POLYPEPTIDES HAVING CELLULASE ACTIVITY - The present disclosure relates to CBH II chimera fusion polypetides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.10-07-2010
20090004698METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.01-01-2009
20100221784Beta-Glucosidase Enhanced Filamentous Fungal Whole Cellulase Compositions and Methods Of Use - The present disclosure provides beta-glucosidase enhanced filamentous fungal whole cellulase compositions. Also provided are methods of hydrolyzing a cellulosic material with beta-glu-cosidase enhanced whole cellulase compositions. The present disclosure further provides methods of decreasing the amount of a whole cellulase required to hydrolyze a cellulosic material by adding an effective amount beta-glucosidase.09-02-2010
20100304437Methods for enhancing the degradation or conversion of cellulosic material - The present invention relates to methods for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity.12-02-2010
20100311125Method for Liquefying and Saccharifying Starch Quickly - A method for liquefying and saccharifying starch quickly includes the steps of: irradiating starch with 0.25×1012-09-2010
20100317058PROCESS FOR MODIFYING THE THERMAL AND/OR DIGESTION PROPERTIES OF CORN STARCHES AND CORN FLOURS - The present invention relates to processes for modifying the thermal and/or digestion properties of corn starches and corn flours.12-16-2010
20100178677SYSTEM AND PROCESS FOR BIOMASS TREATMENT - A system including an apparatus is presented for treatment of biomass that allows successful biomass treatment at a high solids dry weight of biomass in the biomass mixture. The design of the system provides extensive distribution of a reactant by spreading the reactant over the biomass as the reactant is introduced through an injection lance, while the biomass is rotated using baffles. The apparatus system to provide extensive assimilation of the reactant into biomass using baffles to lift and drop the biomass, as well as attrition media which fall onto the biomass, to enhance the treatment process.07-15-2010
20100159517ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution, under alkaline conditions, in the presence of one or more organo-mercaptan and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.06-24-2010
20110129877PULLULANASE VARIANTS AND METHODS FOR PREPARING SUCH VARIANTS WITH PREDETERMINED PROPERTIES - The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern.06-02-2011
20100136629ENHANCED ETHANOL FERMENTATION USING BIODIGESTATE - Methods and systems for enhancing ethanol production using a suspending fluid are described. The suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms. Also described are methods and systems for hydrolyzing a feedstock for fermentation that include hydrolyzing a feedstock suspension. The feedstock suspension can include feedstock that includes complex sugars, and a suspending fluid, wherein the suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms.06-03-2010
20110250646AMMONIA PRETREATMENT OF BIOMASS FOR IMPROVED INHIBITOR PROFILE - Methods for treating biomass for release of fermentable sugars with an improved inhibitor profile are provided. Specifically, a hydrolysate comprising fermentable sugars with an improved inhibitor profile is obtained by saccharification of a reaction product obtained by pretreating biomass with ammonia under suitable reaction conditions. The pretreated biomass reaction product has an acetamide to acetate molar ratio greater than about 1 and an acetyl conversion of greater than 60%. The acetamide to acetate molar ratio is maintained greater than about 1 throughout saccharification. The hydrolysate may be fermented to a target compound.10-13-2011
20110076724Process, Plant, and Biofuel for Integrated Biofuel Production - This invention relates to a process, a plant, and a biofuel for integrated biofuel production, such as with butanol, biodiesel, and/or sugar product. The integrated process includes the step of removing hexose from a feedstock to form a lignocellulosic material. The process also includes the step of converting the hexose to butanol and/or a biodiesel material, and the step of depolymerizing lignocellulosic material to form pentose and a residue. The process also includes the step of converting the pentose to butanol and/or a biodiesel material.03-31-2011
20110076725Lignin Blockers And Uses Thereof - Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein.03-31-2011
20110151516PROCESS FOR THE EXTRACTION OF SUGARS AND LIGNIN FROM SOLID BIOMASS - A process for the extraction of sugars and/or lignin from lignocellulose-containing solid biomass is provided.06-23-2011
20080274509PROCESS FOR PREPARING SUGAR-CONTAINING HYDROLYZATES FROM LIGNOCELLULOSE - A sugar-containing hydrolyzate is produced from a lignocellulose-containing material by a) pretreating the lignocellulose-containing material with a chemical compound selected from sulphuric acid, alkali, peroxodisulphates, potassium peroxide, potassium hydroxide, and mixtures thereof, in the presence of water, thereby obtaining an aqueous phase, and b) after removing of the aqueous phase and washing the resulting product, treating said product with an enzyme suitable for hydrolysis in the presence of water, thereby obtaining a hydrolyzate, the hydrolyzate being suitable as a carbon source for fermentation.11-06-2008
20100304439PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.12-02-2010
20100304440PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.12-02-2010
20090023187Method of obtaining a product sugar stream from cellulosic biomass - A process for obtaining a product sugar stream from cellulosic biomass is disclosed. In one process, the cellulosic biomass is pretreated at a pH between about 0.4 to 2.0 by adding one or more than one acid to produce a pretreated cellulosic biomass comprising acetic acid. One or more than one base is then added to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0 to produce a neutralized cellulosic biomass comprising inorganic salt and acetate salt. The neutralized biomass is then hydrolyzed by cellulase enzymes to produce a crude sugar stream. Insoluble residue is separated from the crude sugar stream and the resulting clarified sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more raffinate streams and a product stream. The raffinate stream comprises inorganic salts and acetate salts, and the product stream comprises sugar. The product stream may then be fermented or otherwise further processed. In an alternate process, a product sugar stream is obtained from a crude sugar stream that is produced from conversion of cellulosic biomass to sugar. The cellulosic biomass may be produced using any suitable method. In this process the crude sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, and the product sugar stream is obtained.01-22-2009
20080248532Enzymatic Method for Producing Dihydroxyacetone Phosphate - A method for making dihydroxyacetone phosphate (DHAP), comprising treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate. The invention further pertains to a method for stereospecifically making, preferably in one pot, a compound of the formula:10-09-2008
20090317872ACID FUNGAL PROTEASE IN FERMENTATION OF INSOLUBLE STARCH SUBSTRATES - The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.12-24-2009
20120231507NOVEL CELLULASES WITH HIGH ACTIVITIES - Two novel cellulases and nucleotide sequences encoding the same are disclosed. Also disclosed are compositions and methods for using the same for hydrolyzing cellulosic waste materials.09-13-2012
20110020873Biomass Hydrolysis Process - The invention relates to a biomass process comprising removal and/or inactivation of an enzyme inhibitor from recycled washing solution.01-27-2011
20110020874Simplified Method for Digestion of Cellulosic Biomass - The inventive process converts cellulosic biomass into a gel-like state that is readily hydrolyzed by appropriate enzymes. First the biomass is mechanically reduced in size. The biomass is then mixed and kneaded with an aqueous solution of a hydrophilic polymer that acts as a conditioning agent or as a co-solvent. During mixing the cellulose (and hemicellulose) in the biomass swells and becomes hydrated forming a viscous gel-like material. The processed material can then be thinned through the addition of water whereupon hydrolytic enzymes are mixed into the material and rapid hydrolysis into free sugars takes place. Dextrins are effective hydrophilic polymers for conditioning biomass. Polyvinyl alcohol is a particularly effective conditioning agent for use with biomass when converted into a viscous gel by adding borate ions.01-27-2011
20100285534COMBINED THERMOCHEMICAL PRETREATMENT AND REFINING OF LIGNOCELLULOSIC BIOMASS - One aspect of the present invention relates to a method of processing lignocellulosic material, comprising initial steam pretreatment to give pretreated lignocellulosic material with an average particle size, followed by refining to give refined lignocellulosic material with an average particle size, wherein the average particle of the pretreated lignocellulosic material is greater than the average particle size of the refined lignocellulosic material. In certain embodiments, the lignocellulosic material is selected from the group consisting of grass, switch grass, cord grass, rye grass, reed canary grass, miscanthus, sugar-processing residues, sugar cane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, recycled wood pulp fiber, sawdust, hardwood, and softwood.11-11-2010
20100167351Integrated Methods for Processing Palm Fruit Bunches - This invention is directed to an integrated method for the processing of palm fruit bunches to oil and other products. The method comprises inter alia separating palm fruit carrying bunches into fruits and lignocellulosic empty fruit bunches, processing the fruits to form palm oil, and at least one lignocellulosic processing coproduct; generating an aqueous stream; producing a non-oil, non-alcohol, non-fatty acid ester third product from the oil, the lignocellulosic processing coproduct, the aqueous stream or from a combination thereof; processing at least a portion of the lignocellulosic empty fruit bunches, lignocellulosic processing coproduct or a combination thereof into a fourth product and optionally producing at least one fifth conversion product from the fourth product; and using at least a portion of the fourth product or a product of its conversion or a combination thereof.07-01-2010
20100159520ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of ammonia and optionally an additional nucleophile to fragment and extract lignin without loss of hemicellulose. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.06-24-2010
20100159515OXIDATIVE PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Lignocellulosic biomass comprising lignin is treated by selective extraction and oxidation of lignin using a solvent solution comprising water in combination with at least one Mn(III) salt to produce readily saccharifiable carbohydrate enriched biomass.06-24-2010
20100159521OZONE TREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Methods for treating lignocellulosic biomass to produce readily saccharifiable carbohydrate-enriched biomass are provided. In one method, lignocellulosic biomass comprising lignin is treated with aqueous ammonia, then contacted with a gas comprising ozone at a temperature of about 0° C. to about 50° C. In another method, lignocellulosic biomass comprising lignin is contacted with a gas comprising ozone at a temperature of about 0° C. to about 50° C., then treated with aqueous ammonia. The readily saccharifiable carbohydrate-enriched biomass may be saccharified with an enzyme consortium to produce fermentable sugars.06-24-2010
20100159518ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one of more sulfide (hydrosulfide) salt and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.06-24-2010
20100159516ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one or more alkylamine and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.06-24-2010
20090176277METHOD FOR PREPARING CELLULOSE DERIVATIVES HAVING SOLUBILITY IMPROVED - Provided is a method for preparing a cellulose derivative having solubility improved and therefore having less undissolved floating portions when the derivative is added into water. More specifically, provided is a method for preparing a cellulose derivative, comprising a step of depolymerizing a cellulose derivative to produce a depolymerized cellulose derivative having a viscosity at 20° C. in a 2% by weight aqueous solution of the depolymerized cellulose derivative reduced by at least 10% compared with that of the cellulose derivative before the depolymerization so that the number of undissolved floating portions in the aqueous solution of the depolymerized cellulose derivative is decreased compared with that of the cellulose derivative before the depolymerization. Depolymerization is effected preferably by an acid, alkali or enzyme.07-09-2009
20100159519ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of elemental sulfur and optionally one or more alkylamine and/or one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.06-24-2010
20110136176METHOD FOR TREATING LIGNOCELLULOSE RAW MATERIAL - A method for treating a lignocellulose raw material so as to facilitate a pulverization treatment that is necessary for the efficient and effective use of a lignocellulose raw material as a raw material or resource for material conversion. When converting a lignocellulose raw material into sugar or a useful material, such as ethanol, with the aid of an enzyme or producing a biodegradable material derived from a lignocellulose raw material via a mechanical or chemical treatment, a lignocellulose raw material is treated with an enzyme prior to or simultaneously with the pulverization process, so that the viscosity of slurry comprising a lignocellulose raw material and water is lowered and the pulverization efficiency is improved.06-09-2011
20110262970Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Cationic Polysaccharides - A method for producing a fermentation product from a lignocellulose-containing material comprises pre-treating the lignocellulose-containing material; introducing a cationic polysaccharide to the pre-treated lignocellulose-containing material; exposing the pre-treated lignocellulose-containing material to an effective amount of a first hydrolyzing enzyme; and fermenting with a fermenting organism to produce a fermentation product. The cationic polysaccharide may be a cationic starch.10-27-2011
20100159522ORGANOSOLV AND OZONE TREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Lignocellulosic biomass comprising lignin is treated with a solvent, such as organosolv, under alkaline conditions at elevated temperatures, filtered, then contacted with a gas comprising ozone to produce a readily saccharifiable biomass.06-24-2010
20100297705PROCESSING BIOMASS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a fluid medium and a saccharifying agent.11-25-2010
20110081683Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes - A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from 04-07-2011
20120149065USE OF MANGANESE PEROXIDASE FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL - Improved manganese peroxidases, polynucleotides encoding improved manganese peroxidase and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved manganese peroxidases and cells expressing a heterologous manganese peroxidase as disclosed herein.06-14-2012
20100184150NUTRITIONAL COMPOSITIONS - The present invention is directed to a novel saccharide composition isolated from a yeast culture. The invention is also directed to yeast cell based saccharide composition with increased water-solubility. The saccharide compositions disclosed in the invention can be used as nutritional or pharmaceutical additives or as part of nutritional or pharmaceutical composition in order to promote the health of an animal or human subject to which said composition is administered. The invention is also directed to methods of producing said saccharide compositions.07-22-2010
20090203079Transgenic monocot plants encoding beta-glucosidase and xylanase - Plant proteins isolated from monocot plants from transformation of the monocot plant with DNA at least 80% homologous to the bglA gene encoding β-glucosidase from a rumen bacterium which is 08-13-2009
20090053770Biomass Pretreatment - A method is provided for producing an improved pretreated biomass product for use in saccharification followed by fermentation to produce a target chemical that includes removal of saccharification and or fermentation inhibitors from the pretreated biomass product. Specifically, the pretreated biomass product derived from using the present method has fewer inhibitors of saccharification and/or fermentation without a loss in sugar content.02-26-2009
20100151527FINE FIBROUS CELLULOSIC MATERIAL AND PROCESS FOR PRODUCING THE SAME - [Problem] To provide a fine fibrous cellulosic material capable of producing a saccharide in a high yield by hydrolysis; to provide a process for producing the fine fibrous cellulosic material from a cellulosic material; and to provide a process for producing the saccharide using the fine fibrous cellulosic material.06-17-2010
20120009626BIOMASS PROCESSING SYSTEM AND SACCHARIDE-SOLUTION PRODUCTION METHOD USING BIOMASS MATERIAL - A hydrothermal decomposition apparatus 01-12-2012
20110165625Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from 07-07-2011
20120064574METHOD FOR CONVERTING LIGNOCELLULOSIC BIOMASS - The present invention aims to develop a pretreatment technology for performing efficient saccharification without losing carbohydrates (in particular, free carbohydrates, starch, xylan, or the like) due to solid-liquid separation and washing steps, as a pretreatment for enzymatic saccharification of a lignocellulosic biomass feedstock (including a lignocellulosic biomass feedstock containing readily degradable carbohydrates). Provided are: a production method for a slurry to be used as a substrate for an enzymatic saccharification reaction, comprising: pulverizing an aerial part of a plant as a lignocellulosic biomass feedstock; preparing a slurry containing the biomass feedstock, calcium hydroxide, and water; subjecting the slurry to an alkali treatment; and neutralizing the slurry by introduction of and/or pressurization with carbon dioxide to decrease a pH to 5 to 7; an enzymatic saccharification method, comprising using, as a substrate, a slurry obtained by the production method for a slurry; and a production method for ethanol, comprising using, as a substrate, a saccharification product obtained by the enzymatic saccharification method.03-15-2012
20120156727Pretreatment of Non-Wood Lignocellulosic Material - The present disclosure provides a method for pre-treating non-wood lignocellulosic material containing less than 5 % (w/w) starch or sugar in a process for production of ethanol from lignocellulose, comprising the steps of: adding organic acid or organic acid-producing bacteria to the lignocellulosic material; storing the lignocellulosic material in the presence of the organic acid for a period of at least two weeks in an atmosphere of less than 5% oxygen to obtain organic acid-impregnated material; and heating the organic acid-impregnated material at a temperature of at least 160° C. to obtain pre-treated lignocellulosic material, wherein no, or substantially no, inorganic acid or base, including SO06-21-2012
20110091938Starch Hydrolysis - Disclosed is a method for hydrolyzing starch. The starch is subjected to hydrolysis in the presence of a neutral calcium salt, the hydrolysis being conducted at a pH ranging from about 5.0 to about 5.5. The calcium salt may be a non-toxic and label-friendly salt such as calcium sulfate, calcium chloride or calcium phosphate, preferably naturally existing salts. The enzymatically catalyzed hydrolysis may be quenched using an organic acid, such as citric acid, preferably an acid that exists in nature, or with heat. Certain processing and product quality advantages may be realized via the disclosed method. The method may provide a variety of hydrolyzed products, including maltodextrins, syrup solids, and the like.04-21-2011
20110104759ENZYMES FOR STARCH PROCESSING - The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.05-05-2011
20100093040MODIFIED BETA-GLUCOSIDASES WITH IMPROVED STABILITY - Provided are modified beta-glucosidase enzymes, derived from the 04-15-2010
20110003341PROCESS FOR PRODUCING SACCHARIDE - A process for producing saccharide, including saccharifying decrystallized cellulose prepared from a raw material containing cellulose having cellulose I-type crystallinity of more than 33%, the process including: treating the cellulose-containing raw material by means of a mill to reduce the cellulose I-type crystallinity of the cellulose to 33% or less, wherein the cellulose-containing raw material has a cellulose content of a residue obtained by removing water from the cellulose-containing raw material of 20% by weight or more, to thereby prepare decrystallized cellulose, and causing a cellulase and/or a hemicellulase to act on the decrystallized cellulose.01-06-2011
20120214205PLANT BIOMASS PRETREATMENT METHOD - A plant biomass pretreatment method which allows prompt pretreatment of plant biomass with simple equipment is provided. The method includes continuously performing in sequence, inside an extruder, pretreatment steps of coarsely crushing the plant biomass to a predefined size or smaller, adding a decomposing agent(s) to the coarsely crushed plant biomass, applying a hot compressed water treatment(s) to the plant biomass with the decomposing agent added thereto, and performing saccharification preparation for mixing the plant biomass with the hot compressed water treatment applied thereto with an enzyme(s) for saccharifying the plant biomass.08-23-2012
20120135467HMO SYNTHESIS - The present invention relates to a cell to be stably cultured in a medium, which cell is adjusted for the production of oligosaccharides, the cell being transformed to comprise at least one nucleic acid sequence coding for an enzyme involved in oligosaccharide synthesis. In addition the cell is transformed to comprise at least one nucleic acid sequence coding for a protein of the sugar efflux transporter family, a functional homolog or derivative thereof. Further, the invention concerns a method for the production of oligosaccharides involving above cell.05-31-2012
20120135465Endoglucanase For Reducing The Viscosity Of A Plant Materials Slurry - The present disclosure relates to composition comprising EG cellulase and methods of use, thereof. The compositions are useful, e.g., for reducing the viscosity of plant material slurry.05-31-2012
20120135468BETA-GALACTOSIDASE DERIVED FROM BACILLUS CIRCULANS - Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from 05-31-2012
20120135466Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant And A Debranching Enzyme - The combination of a 05-31-2012
20120252070BIOMASS SHOCK PRETREATMENT - Methods and apparatus for treating biomass that may include introducing a biomass to a chamber; exposing the biomass in the chamber to a shock event to produce a shocked biomass; and transferring the shocked biomass from the chamber. In some aspects, the method may include pretreating the biomass with a chemical before introducing the biomass to the chamber and/or after transferring shocked biomass from the chamber.10-04-2012
20100273217BIOCABURANT PREPARATION USING PENCILLIUM FUNICULOSUM ENZYMES - The present invention deals with a method for treating biomass comprising the steps of providing an enzyme mixture obtained from Penicillium funiculosum deposited under Budapest treaty in the International Mycological Institute under the number IMI 378536, providing plant biomass then contacting the enzyme mixture of step (a) and the biomass of step (b) under conditions wherein the saccharification of the biomass occurs.10-28-2010
20090061486METHOD FOR CELLULASE PRODUCTION - A fermentation process using hemicellulose-derived carbohydrates for the production of cellulase mixtures with a high proportion of cellulases relative to hemicellulases is provided. The cellulases produced by the process of the invention are further characterized by high specific productivity. The resulting cellulase mixtures comprise at least two times more cellulase than hemicellulase and are useful for the hydrolysis of cellulosic substrated, particularly, pretreated lignocellulosic substrate.03-05-2009
20120225455Method for Producing Sugar - Disclosed is a process for producing a sugar, whereby any non-sugar component can be removed from a sugar solution with high efficiency and the color value of the sugar solution can be reduced. Specifically disclosed is a process for producing sugar, which is characterized by comprising: adding ethanol to a sugar solution produced by squeezing a plant; removing precipitates produced by the addition of ethanol from the solution; and crystallizing sugar from the sugar solution from which the precipitates have been removed.09-06-2012
20120190072PROTEIN - The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.07-26-2012
20120264173ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one or more alkylamine and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.10-18-2012
20090017503Method and Apparatus for Saccharide Precipitation From Pretreated Lignocellulosic Materials - A method for separating saccharide components and lignin fractions from a concentrated acid treated lignocellulosic biomass is disclosed. The method involves precipitating the saccharide components by adding an organic solvent to the biomass slurry. The acid may then be recovered, for example, by filtration or by countercurrent washing and the organic solvent may be flashed and recycled. During acid recovery and organic recovery steps, two main lignocellulose components (hemicellulose and lignin) as well as minor components such as acetic acid are separated as well. The method decreases the amount of cellulase required for hydrolysis, increases hydrolysis rates, reduces formation of inhibitor molecules, increase sugar yields, produces high value by-products such as high quality lignin and hemicellulose, and decreases energy and equipment costs.01-15-2009
20120270269ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION - Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of ammonia and optionally an additional nucleophile to fragment and extract lignin without loss of hemicellulose. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.10-25-2012
20100203593 CIP1 Polypeptides and their uses - Described herein are novel gene sequences isolated from 08-12-2010
20110250645METHODS TO IMPROVE MONOMERIC SUGAR RELEASE FROM LIGNOCELLULOSIC BIOMASS FOLLOWING ALKALINE PRETREATMENT - A method is provided for improving the release of monomeric sugars from alkaline pretreated biomass. The method includes further processing of pretreated biomass and addition of a chemical to the saccharification reaction, which together provides for unexpected release of high levels of monomeric sugars that may be fermented to target products.10-13-2011
20110294165VARIANT HUMICOLA GRISEA CBH1.1 - Disclosed are variants of 12-01-2011
20120329096RECOVERY OF INORGANIC SALT DURING PROCESSING OF LIGNOCELLULOSIC FEEDSTOCKS - A method for recovering inorganic salt during processing of a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock by adding an acid to the feedstock to produce a pretreated lignocellulosic feedstock. A soluble base is then added to the pretreated lignocellulosic feedstock to adjust the pH and produce a neutralized feedstock. The neutralized feedstock is then enzymatically hydrolyzed to produce an enzyme hydrolyzed feedstock and a sugar stream. Inorganic salt is recovered from either a stream obtained from the lignocellulosic feedstock prior to the step of pretreating, a stream obtained from the pretreated lignocellulosic feedstock, a stream obtained from the neutralized feedstock, a stream obtained from the sugar stream, or a combination of these streams. The inorganic salt may be concentrated, clarified, recovered and purified by crystallization, electrodialysis drying, or agglomeration and granulation, and then used as desired, for example as a fertilizer.12-27-2012
20120100578CELLULASE PREPARATION COMPRISING ENDOGLUCANASES DERIVED FROM TWO DIFFERENT TYPES OF MICROORGANISMS - By having a cellulase preparation comprising at least a certain amount of endoglucanases derived from two different types of microorganisms, the cellulase preparation can be provided with a higher activity and a wider pH property than those of cellulase preparations each containing one of the endoglucanases alone. Moreover, by introducing and expressing simultaneously two different types of cellulase genes in a single host cell, a cellulase preparation having a high activity and a wide pH property can be produced easily.04-26-2012
20120100577PROCESSING BIOMASS - Methods of manufacturing fuels are provided. These methods use often difficult to process lignocellulosic materials, for example crop residues and grasses. The methods can be readily practiced on a commercial scale in an economically viable manner, in some cases using as feedstocks materials that would otherwise be discarded as waste.04-26-2012
20100167349ORF11 FROM CAMPYLOBACTER JEJUNI IS A SIALATE-O-ACETYLTRANSFERASE - The invention relates to sialate-O-acetyltransferase (SOAT) polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.07-01-2010
20100167350MICROORGANISM WITH ABILITY TO PRODUCE DEOXY POLYOL DEHYDROGENASE AND USE THEREOF - Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.07-01-2010
20110159545Fungamyl-like Alpha-Amylase Variants - The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.06-30-2011
20110159544Method for treating cellulosic material and CBHII/CEL6A enzymes useful therein - The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.06-30-2011
20130143271NOVEL EXPRESSION-REGULATING SEQUENCES AND EXPRESSION PRODUCTS IN THE FIELD OF FILAMENTOUS FUNGI - The invention pertains to novel proteins corresponding to 06-06-2013
20110269188Polypeptides having feruloyl esterase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having feruloyl esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.11-03-2011
20130130318PROCESS FOR OBTAINING BIOCHEMICALS IN A ZERO-LIQUID DISCHARGE PLANT - A method is presented for the production of cellulosic ethanol, acetic acid and derivatives from the extract containing fibers and hemicelluloses after steam cooking of biomass in a host plant. The process is integrated with the host plant process to minimize the effect of loss of heat value from the extracted hemicelluloses and eliminate the need for the waste water treatment plant.05-23-2013
20080206821Method for Manufacturing Xylitol with High-Yield and High-Productivity - The present invention provides a method for manufacturing xylitol with high-yield and high-productivity by using a xylitol dehydrogenase-deficient mutant of xylitol producing microorganism. This goal is achieved through modification of the metabolic pathway of the xylitol producing microorganism, preferably a natural xylose-assimilating yeasts and fungi, by disrupting or inactivating the expression of desired genes.08-28-2008
20130203117MANUFACTURING METHOD FOR SUGAR SOLUTION AND DEVICE FOR SAME - A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.08-08-2013
20100297704PROCESS FOR BIOMASS CONVERSION - The present invention relates to a clean process of preparing high grade biomass products, and their use in the production of health care products, bio-energy products, biochemicals, bio-originated chemicals and biodegradable plastics.11-25-2010
20100304438NOVEL BETA-GLUCOSIDASE ENZYMES - Provided are modified beta-glucosidase enzymes, derived from the 12-02-2010
20100317059BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES - The invention provides variants of the 12-16-2010
20120282656MULTISTAGE FRACTIONATION PROCESS FOR RECALCITRANT C5 OLIGOSACCHARIDES - Methods are disclosed for increasing the level of C11-08-2012
20120282655ENHANCED SOLUBLE C5 SACCHARIDE YIELDS - Methods are disclosed for increasing the level of soluble C11-08-2012
20130183715Method and Apparatus for Producing Engineered Fuel from High Cellulose Feedstock - An apparatus and method for producing methane gas, synthetic hydrocarbon gas, and fertilizer is provided. The apparatus includes a mix tank for mixing cellulosic material with a solvent into a slurry and a generator having an exhaust. The apparatus further includes a stir tank reactor for converting the slurry to a solution containing lignin-like carbon and liquid, and a separator for separating the lignin-like carbon and liquid. An anaerobic digester decomposes the received liquid received from the stir tank into methane and liquid components. A carbon dioxide scrubber scrubs the methane component of carbon dioxide.07-18-2013
20110312033METHODS OF SPRAYING SACCHARIFICATION ENZYMES AND FERMENTATION ORGANISMS ONTO LIGNOCELLULOSIC BIOMASS FOR HYDROLYSIS AND FERMENTATION PROCESSES - The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.12-22-2011
20130189738FIBROUS MATERIALS AND COMPOSITES - Fibrous materials, compositions that include fibrous materials, and uses of the fibrous materials and compositions are disclosed. For example, the fibrous materials can be operated on by a microorganism to produce ethanol or a by-product, such as a protein or lignin.07-25-2013
20130189739Biological Reduction of Carbon Dioxide Pollutants Systems and Methods - Methods and systems to achieve clean fuel processing systems in which carbon dioxide emissions (07-25-2013
20120028306POLYPEPTIDES WITH XYLANASE ACTIVITY - Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of 02-02-2012
20090176278SOLVENTS FOR MUTANT ENDOGLYCOCERAMIDASES WITH SYNTHETIC ACTIVITY - The present invention provides reaction mixtures comprising a solvent having at least one of an alkoxy ether and/or a polyhydric alcohol for use in reactions with a mutant endoglycoceramidase having enhanced synthetic activity.07-09-2009
20130095528BIFIDOBACTERIAL GENE SEQUENCES AND THEIR USE - This invention provides nucleic acids and proteins involved in oligosaccharide modification in the species Bifidobacteria. The invention provides methods for utilizing the proteins of the invention to generate human milk oligosaccharides or oligosaccharide mimics. The invention also provides compositions containing the human milk oligosaccharides or oligosaccharide mimics and methods for use.04-18-2013
20120088274Polypeptides having Beta-Glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.04-12-2012
20120088273NOVEL CELLULOSE AND LIGNO-CELLULOSE ACTIVE PROTEINS - The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X (residues 1-12 of SEQ ID NOS:6-7). The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X04-12-2012
20120094331METHOD FOR THE PREPARATION OF CARBOHYDRATE CLEAVAGE PRODUCTS FROM A LIGNOCELLULOSIC MATERIAL - A method for the preparation of carbohydrate cleavage products, characterized by the combination of the measures that the lignocellulosic material is treated with an aqueous solution containing hydrogen peroxide, an alcohol, in particular a C04-19-2012
20120094330Incorporation of Flavan-3-ols and Gallic Acid Derivatives into Lignin to Improve Biomass Utilization - A method of manufacturing modified lignin and the resulting non-natural modified lignin product in which a lignin-producing polymerization reaction is performed using a polymerizable monomer having the structure:04-19-2012

Patent applications in class Preparing compound containing saccharide radical

Patent applications in all subclasses Preparing compound containing saccharide radical