| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435690400 | Hormones and fragments thereof | 67 |
| 20130045506 | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 02-21-2013 |
| 20110177555 | CHINESE HAMSTER OVARY CELL LINES - We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with | 07-21-2011 |
| 20090011463 | Pcka Modifications and Enhanced Protein Expression in Bacillus - The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins, wherein the pckA gene has been modified or deleted. In particular, the present invention relates to Gram-positive microorganisms, such as | 01-08-2009 |
| 20110189733 | LOW CELL DENSITY FERMENTATION PROCESS FOR THE PRODUCTION OF HETEROLOGOUS RECOMBINANT PROTEINS IN MICROORGANISMS - A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD | 08-04-2011 |
| 20090123971 | Compositions of aminoacyl-tRNA synthetase and uses thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs. | 05-14-2009 |
| 20090093023 | Elements for improved expression of bovine somatotropin - The invention allows improved expression of heterologous polypeptides such as bovine somatotropin (bST). Novel compositions and methods are provided for production of bST from a native bST cDNA in transformed host cells such as | 04-09-2009 |
| 20130122546 | NOVEL SIGNAL PEPTIDE, AND USE THEREOF FOR PRODUCING RECOMBINANT PROTEINS - A use of a signal peptide for producing a recombinant polypeptide of interest in an expression system, the signal peptide includes at least 12 amino acids of formula (I): | 05-16-2013 |
| 20130122547 | YEAST HOST, TRANSFORMANT AND METHOD FOR PRODUCING HETEROLOGOUS PROTEINS - A method of constructing a host for expression of an exogenous gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes of | 05-16-2013 |
| 20130122548 | Eubacterial RNA-Polymerase Mutants With Altered Product Production - The present invention relates to an isolated mutant | 05-16-2013 |
| 20100311122 | VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION - Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2 | 12-09-2010 |
| 20090275084 | LONG-ACTING EPO POLYPEPTIDES AND DERIVATIVES THEREOF AND METHODS THEREOF - A polypeptide and polynucleotides encoding same comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to an EPO peptide are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed. | 11-05-2009 |
| 20080254511 | PROCESS FOR THE FERMENTATIVE PRODUCTION OF PROTEINS - The present invention relates to a process for producing a heterologous protein by means of an | 10-16-2008 |
| 20110287483 | PRODUCTION OF GLYCOPROTEINS USING MANGANESE - Culture media comprising manganese and methods of culturing cells to improve sialylation and glycosylation of glycoproteins are provided. | 11-24-2011 |
| 20110294161 | Modified Human Growth Hormone - Modified growth hormone polypeptide and uses thereof are provided. | 12-01-2011 |
| 20100021966 | Method for the Preparation of Growth Hormone and Antagonist Thereof Having Lower Levels of Isoform Impurities Thereof - The present invention is directed generally to recombinant methods for making a desired polypeptide. These method(s) yield a polypeptide product containing reduced levels of isoform impurities thereof. In particular, the present invention is directed to (1) a recombinant method for preparing growth hormone with reduced isoform impurities thereof and (2) a recombinant method for preparing a growth hormone antagonist, such as pegvisomant, and its protein intermediate, also having reduced isoform impurities thereof. More specifically, the isoform impurities that are decreased by methods of the present invention are the trisulfide and des-phe isoform impurities of growth hormone and growth hormone antagonist (or its intermediate), respectively. | 01-28-2010 |
| 20120190069 | GLUTAMINE-AUXOTHROPHIC HUMAN CELLS CAPABLE OF PRODUCING PROTEINS AND CAPABLE OF GROWING IN A GLUTAMINE-FREE MEDIUM - A glutamine-auxotrophic human cell transfected with an exogenous DNA sequence encoding a protein or an exogenous DNA sequence capable of altering the expression of an endogenous gene encoding a protein and an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more than one DNA construct, said transfected cell capable of producing said protein and capable of growing in a glutamine-free medium. | 07-26-2012 |
| 20120034655 | FSH PRODUCING CELL CLONE - The present invention relates to nucleic acid molecules comprising a nucleic acid sequence coding for the α- and the β-chain of the human follicle stimulating hormone (FSH), respectively, which has been modified with respect to the codon usage in CHO cells. The present invention further relates to a recombinant nucleic acid molecule comprising such nucleic acid sequences and host cells containing such recombinant nucleic acid molecules, as well as their use in the production of recombinant human FSH. Finally, the present invention also relates to a method for producing host cells expressing human follicle stimulating hormone by transfecting cells in suspension culture under serum-free conditions with the recombinant nucleic acid molecule of the present invention. | 02-09-2012 |
| 20090017497 | METHOD FOR MAKING INSULIN PRECURSORS AND INSULIN PRECURSOR ANALOGUES HAVING IMPROVED FERMENTATION YIELD IN YEAST - Novel insulin precursors and insulin precursor analogs comprising a connecting peptide (mini C-peptide) of preferably up to 15 amino acid residues and comprising at least one Gly are provided. The precursors can be converted into human insulin or a human insulin analog. The precursors will typically have a distance between B27 (atom CG2) and A1 (atom CA) of less than 5 Å. | 01-15-2009 |
| 20110171687 | UNSTRUCTURED RECOMBINANT POLYMERS AND COMPOSITIONS COMPRISING SAME - The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications. | 07-14-2011 |
| 20080206816 | YEAST HOST, TRANSFORMANT AND METHOD FOR PRODUCING HETEROLOGOUS PROTEINS - The efficiency of production of a heterologous protein by a transformant of a yeast host is improved. | 08-28-2008 |
| 20090170163 | SPACERS TO INCREASE THE EXPRESSION OF RECOMBINANT FUSION PROTEINS - The present invention relates to fusion proteins. The invention specifically relates to compositions and methods of Tf-based fusion proteins that demonstrate a high-level expression of transferrin (Tf)-based fusion proteins by inserting a helical linker between two protein domains. | 07-02-2009 |
| 20090017498 | Fibroblast Growth Factor-Like Polypeptides - The present invention provides novel Fibroblast Growth Factor-like (FGF-like) polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing FGF-like polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with FGF-like polypeptides. | 01-15-2009 |
| 20090203076 | COMPOSITIONS AND METHODS FOR INCREASING PROTEIN PRODUCTION - Compositions and methods for increasing protein production are provided. | 08-13-2009 |
| 20090239262 | Affinity Polypeptide for Purification of Recombinant Proteins - The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography. | 09-24-2009 |
| 20100184141 | Serum-free Stable Transfection and Production of Recombinant Human Proteins in Human Cell Lines - The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum-free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself. | 07-22-2010 |
| 20100261227 | Production of Proteins Using Transposon-Based Vectors - Novel compositions for the in vitro or in vivo production of specific proteins are provided. The compositions comprise components of vectors, such as a vector backbone, a promoter, and a gene of interest that encodes for the protein of interest, and the transposon-based vectors comprising these components. Also provided are methods of making these compositions and methods of using these compositions for the production of desired proteins in vivo or in transfected cells in vitro. | 10-14-2010 |
| 20120196326 | Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture - Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells. | 08-02-2012 |
| 20090111143 | Methods and Compositions for Mammalian Cell Lines for Transfection and Protein Expression in Serum-Free Medium - Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transfection under serum-free conditions. In preferred embodiments, the cells of use are SpESF or SpESF-X cells. | 04-30-2009 |
| 20110111460 | Method for Making Mature Insulin Polypeptides - The invention is related to a method for making human insulin analogues by culturing an fungi cell comprising a DNA vector encoding a precursor for human insulin analogue, wherein the said precursor comprises a connecting peptide flanked with cleavage sites at both junctions with the A- and the B-chain of the insulin peptide, respectively said cleavage sites being cleaved within the fungi cell allowing the cell to secrete high amount of correctly processed, mature two chain human insulin analogue to the culture media. | 05-12-2011 |
| 20110212488 | IMMORTALIZED AVIAN CELL LINES AND USE THEREOF - The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (W—COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxyiridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins. | 09-01-2011 |
| 20110008839 | Expression Vectors Comprising the MCMV IE2 Promoter - The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and/or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein the expression vector does not contain any complete gene of the mCMV. | 01-13-2011 |
| 20100255537 | NUCLEIC ACID MOLECULES ENCODING BETA-LIKE GLYCOPROTEIN HORMONE POLYPEPTIDE AND HETERODIMER THEREOF - Novel β10 polypeptides and heterodimers thereof, and nucleic acid molecules encoding the same are disclosed. The invention also provides vectors, host cells, selective binding agents, and methods for producing β10 polypeptides and heterodimeric forms thereof, specifically α2/β10. Also provided for are methods for the treatment, diagnosis, amelioration, or prevention of diseases with β10 polypeptides and α2/β10 heterodimers or their respective binding agents. | 10-07-2010 |
| 20090317867 | METHODS FOR ENHANCED PRODUCTION OF BONE MORPHOGENETIC PROTEINS - Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-β superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 μM and if pyridoxal is present, it makes up less than 55% of the molar concentration of vitamin B6 in the media. | 12-24-2009 |
| 20080199909 | Methods for Expression and Purification of Recombinant Human Growth Hormone - The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules. | 08-21-2008 |
| 20110020871 | Process for the Preparation of Insulin Conjugates - The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in | 01-27-2011 |
| 20100184142 | BACTERIA WITH INCREASED LEVELS OF PROTEIN SECRETION, NUCLEOTIDE SEQUENCES CODING FOR A SECA PROTEIN WITH INCREASED LEVELS OF PROTEIN SECRETION, AND METHOD FOR PRODUCING PROTEINS - The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion and containing a gene sequence SecA or an allele, homologue or derivative of said nucleotide sequences or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time. | 07-22-2010 |
| 20120309053 | THREE DIMENSIONAL POROUS STRUCTURES - A 3-dimensional porous polymeric structure comprising a porous polymer structure optionally with particles within the pores of the polymer and wherein the pores have a narrow pore-size distribution. The structure may be made by closely packing particles in a zone to provide a 3-dimensional array, contacting a polymerisable monomer with the array such that the composition fills interstitial spaces between the particles and effecting polymerisation of the monomer whereby a polymer structure is formed around the particles and optionally removing the particles from the structure. The 3-dimensional porous structure may be used in solid phase synthesis, immobilisation, cell culturing and preparation of a stationary phase for chromatographic separation, as an absorbent, an insulating material or in tissue regeneration. | 12-06-2012 |
| 20110189732 | Process for the Fermentative Production of Erythropoietin - The present invention relates to a process for the fermentative continuous production of erythropoietin, where eukaryotic erythropoietin-producing cells are cultured in a perfusion reactor while retaining the cells, the glucose concentration in the culture supernatant being adjusted via the perfusion rate and the cell number via the cell retention rate and/or the outward transferal of defined amounts of cell-containing culture medium from the bioreactor within preset zones. | 08-04-2011 |
| 20100021967 | Alpha Factor Signal Peptide For Producing a Polypeptide - The present invention relates to a method for producing a polypeptide comprising using a variant alpha factor signal peptide having a substitution in position 9 resulting in a substitution of an A to either T, S, H, I, F, E, or G. The invention further relate to nucleic acid constructs comprising a first nucleotide sequence encoding the variant signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising said nuclei acid construct. | 01-28-2010 |
| 20100047870 | LOW CELL DENSITY FERMENTATION PROCESS FOR THE PRODUCTION OF HETEROLOGOUS RECOMBINANT PROTEINS IN MICROORGANISMS - A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD | 02-25-2010 |
| 20110053221 | RECOMBINANT PRODUCTION OF AUTHENTIC HUMAN PROTEINS USING HUMAN CELL EXPRESSION SYSTEMS - The present invention relates to novel expression cassettes and vectors for efficiently producing authentic recombinant human proteins from stable cultures of novel human cell lines, the authentic recombinant proteins produced therefrom, and antibodies raised against those authentic recombinant proteins. | 03-03-2011 |
| 20110117600 | PROCESS FOR OBTAINING ASPART INSULIN USING A PICHIA PASTORIS YEAST STRAIN - The present invention refers to a method for producing a human insulin analogue with high efficiency and excellent yield, by means of a biotechnological process comprising transformation of a | 05-19-2011 |
| 20090291473 | Method for Production of Recombinant Human FSH - Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order. | 11-26-2009 |
| 20090181430 | PROTEIN FOLDING - The present invention concerns a method for folding a Transforming Growth Factor Beta, or a functional analogue thereof, into a dimeric, biologically active form. The method involves adding solubilized, unfolded monomeric growth factor to a solution containing 2-(cyclohexylamino)-ethanesulfonic acid (CHES) or a functional analogue thereof and a low molecular weight sulfhydryl/disulfide redox system. The solution is then incubated under conditions suitable for generating dimeric biologically active Transforming Growth Factor Beta. | 07-16-2009 |
| 20120171723 | FGF HOMOLOGS COMPOSITIONS AND USES THEREOF - The present invention relates to zFGF5 compositions and methods of using the compositions to proliferate chondrocytes and their progenitors, and to induce deposition of cartilage. zFGF5 compositions are disclosed for treating disorders associated with chondrocytes, such as cartilage injuries and defects. In addition, methods for treating neurological disorders, such as stroke, are disclosed, and methods for using zFGF5 compositions to stimulate growth of cells associated with neurological injury and disease are disclosed. | 07-05-2012 |
| 20120058513 | METHOD FOR PRODUCING HUMAN RECOMBINANT INSULIN - The invention relates to biotechnology and can be used for producing human recombinant insulin for preparing medicinal agents for the treatment of pancreatic diabetes. A variety of recombinant plasmid DNAs which contain an artificial gene and encode the human insulin precursor is proposed. The biosynthesis of a hybrid polypeptide is induced using isopropyl-thiogalactopyranoside so that the post-induction level of the hybrid polypeptide is equal to or greater than 25% of the total cellular protein. According to the claimed procedure, human insulin is produced by cultivating a producer strain containing one of the recombinant plasmids, isolating inclusion bodies, solubilizing and renaturing the fusion protein, and enzymatically degrading and chromatographically purifying said protein. The invention simplifies the process for producing human recombinant insulin and increases the yield thereof. | 03-08-2012 |
| 20120028301 | SOMATIC HYPERMUTATION SYSTEMS - The present application relates to somatic hypermutation (SHM) systems and synthetic genes. Synthetic genes can be designed using computer-based approaches to increase or decrease susceptibility of a polynucleotide to somatic hypermutation. Genes of interest are inserted into the vectors and subjected to activation-induced cytidine deaminase to induce somatic hypermutation. Proteins or portions thereof encoded by the modified genes can be introduced into a SHM system for somatic hypermutation and proteins or portions thereof exhibiting a desired phenotype or function can be isolated for in vitro or in vivo diagnostic or therapeutic uses. | 02-02-2012 |
| 20120252069 | PROCESS FOR THE PURIFICATION OF GLYCOPROTEINS - The present invention relates to a process for the purification of a glycoprotein comprising subjecting a liquid containing said glycoprotein to the steps of: a) reverse phase chromatography, b) size exclusion chromatography, and c) hydrophobic interaction chromatography. Also provided is a manufacturing process for producing a glycoprotein of interest. | 10-04-2012 |
| 20100093036 | Novel Vectors for Production of Growth Hormone - The present invention provides novel compositions to transfect cells for production of growth hormone (GH). These novel compositions also are used to produce germline transgenic birds that can successfully pass the transgene encoding growth hormone to their offspring. These novel compositions include components of vectors such as a vector backbone, a novel promoter, and a gene of interest that encodes for GH, and the vectors comprising these components. In one embodiment these vectors are transposon-based vectors. The present invention also provides methods of making these compositions and methods of using these compositions for the production of GH in vitro and in vivo. In one embodiment the GH is human (h)GH. | 04-15-2010 |
| 20120122155 | IMMORTALIZED AVIAN CELL LINES AND USE THEREOF - The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (W—COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxviridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins. | 05-17-2012 |
| 20120129218 | COFILIN KNOCKDOWN HOST CELLS AND USES THEREOF - The present invention relates to a host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence. The host cell may further comprise a nucleic acid encoding a recombinant protein. The present invention also relates to a method for producing a recombinant protein by the host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence. | 05-24-2012 |
| 20120077228 | OVEREXPRESSION OF AMINOACYL-tRNA SYNTHETASES FOR EFFICIENT PRODUCTION OF ENGINEERED PROTEINS CONTAINING AMINO ACID ANALOGUES - Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine. | 03-29-2012 |
| 20120282653 | METHOD FOR MAKING POLYPEPTIDES - The invention provides an improved method for producing polypeptides with a C-terminal glycine in a yeast transformant being characterized in having a non functional KEX1 gene. The method is in particular well suited to produce polypeptides with an aromatic amino acid residue attached to the C-terminal glycine. The yeast strain may have further non-functional protease genes selected from PEP4, YPS1, MKCI, YPS3, YPS5, YPS6, YPS7, PRB1, STE13 and KEX2. | 11-08-2012 |
| 20100009408 | Induction of Gene Expression Using a High Concentration Sugar Mixture - Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use. | 01-14-2010 |
| 20120190068 | DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR - Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product. | 07-26-2012 |
| 20120329092 | PROCESS FOR THE PURIFICATION OF GLYCOPROTEINS - The present invention relates to a process for the purification of a glycoprotein comprising subjecting a liquid containing said glycoprotein to the steps of:
| 12-27-2012 |
| 20090017496 | Co-expression of multiple protein chains or subunits - A recombinant genetic construct is provided that includes at least two expression cassettes. Each cassette encodes for a chain or subunit of a target protein. The genetic construct preferably targets any expressed protein to the secretory pathway of the host cell. An application of present invention is found in expressing the two chains of human insulin through two separate expression cassettes on the same methylotrophic yeast expression vector. Mature, bioactive human insulin molecules are secreted through this method without resorting to any post-translational cleavage process. | 01-15-2009 |
| 20120231504 | MULTIUSE REACTORS AND RELATED METHODS - A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation. | 09-13-2012 |
| 20120231505 | Gene Expression Technique - The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising:
| 09-13-2012 |
| 20120322107 | METHODS OF INCREASING THE EXPRESSION YIELD OF VITAMIN K-DEPENDENT PROTEINS - The invention encompasses the use of one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor for the expression of one or more functional vitamin K-dependent proteins in cell culture as well as processes for the fermentation of eucaryotic cells expressing one or more vitamin K-dependent proteins wherein one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor are added to the cell culture medium before and/or during the fermentation process. | 12-20-2012 |
| 20100203589 | METHOD FOR OBTAINING ACTIVE BETA-NGF - The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence. | 08-12-2010 |
| 20110229932 | ARTIFICIAL CHROMOSOME VECTOR - According to the present invention there is provided a method of producing a protein in a eukaryotic cell line, comprising the steps of a) providing a backbone of an artificial chromosome, b) recombining the nucleic acid encoding said protein into said backbone to generate an expression vector, c) introducing said expression vector into a eukaryotic host cell line to obtain a eukaryotic expression cell line, d) cultivating said expression cell line to produce said protein, and e) isolating said protein. The invention further relates to respective vectors and transgenic cell lines. | 09-22-2011 |
| 20110236929 | E. COLI BL21 STRAIN LACKING A FUNCTIONAL GROUP II CAPSULAR GENE CLUSTER - The present invention relates to a novel non-pathogenic | 09-29-2011 |
| 20110312032 | VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION - Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2 | 12-22-2011 |
| 20100167347 | Compositions of Aminoacyl-tRNA Synthetase and Uses Thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs. | 07-01-2010 |
| 20130017574 | METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION AND SECRETION OF PROTEINS - Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described. | 01-17-2013 |
| 20110159539 | METHOD FOR PRODUCING RECOMBINANT PROTEINS WITH A CONSTANT CONTENT OF PCO2 IN THE MEDIUM - A method for the recombinant production of a polypeptide in a eukaryotic host cell modified in the citrate cycle is described, wherein the method comprises cultivating the eukaryotic host cell in a suitable medium under conditions which allow the expression of the polypeptide, wherein the content of dissolved CO | 06-30-2011 |
