Inventors list

Assignees list

Classification tree browser

Top 100 Inventors

Top 100 Assignees


Recombinant DNA technique included in method of making a protein or polypeptide

Subclass of:

435 - Chemistry: molecular biology and microbiology

435041000 - MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435690600 Blood proteins 287
435690700 Fusion proteins or polypeptides 86
435690400 Hormones and fragments thereof 73
435690300 Antigens 40
435690500 Lymphokines or monokines 30
435690200 Enzyme inhibitors or activators 9
435690800 Signal sequence (e.g., beta-galactosidase, etc.) 4
20090305353Mutant firefly luciferase, gene, recombinant vector, transformant, and method for production of mutant firefly luciferase - The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase. According to the present invention, a mutant firefly luciferase with increased luminescence intensity compared with that of wild-type firefly luciferases is provided, regarding which (a) through (c) are as follows: 12-10-2009
20120237979Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.09-20-2012
20090148906OPTIMIZED MESSENGER RNA - The present invention is directed to a synthetic nucleic acid sequence which encodes a protein wherein at least one non-common codon or less-common codon is replaced by a common codon. The synthetic nucleic acid sequence can include a continuous stretch of at least 90 codons all of which are common codons.06-11-2009
20080299619TRICHODERMA REESEI GLUCOAMYLASE AND HOMOLOGS THEREOF - The present invention is related to glucoamylases having at least 80% sequence identity to a 12-04-2008
Entries
DocumentTitleDate
20100099144COMBINED USE OF CELL PERMEABLE NANOG AND OCT4 FOR INCREASING SELF-RENEWAL AND SUPPRESSING DIFFERENTIATION OF STEM CELLS - The present invention discloses cell permeable Nanog and Oct4 recombinant proteins that comprise a kaposi fibroblast growth factor 4 (kFGF4)-derived macromolecule transduction domain (MTD). Also disclosed are polynucleotides encoding the cell permeable Nanog and Oct4 recombinant proteins, a method of increasing self-renewal and suppressing differentiation of stem cells by treating the cells in combination with the cell permeable Nanog and Oct4 recombinant proteins, and the combined use of the cell permeable Nanog and Oct4 recombinant proteins for increasing self-renewal and suppressing differentiation of stem cells.04-22-2010
20130045505Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.02-21-2013
20110177554SECRETED FRIZZLED RELATED PROTEIN, SFRP, FRAGMENTS AND METHODS OF USE THEREOF - The invention stems from the discovery that sFRP and fragments thereof can bind to members of the Wnt family of proteins and cause an increase in Wnt biological activity. Furthermore, fragments of sFRP that do not contain the CRD domain are shown to bind to Wnt proteins and modulate Wnt biological activity. Accordingly, the invention provides these sFRP fragments and variants of these fragments, as well as vectors and host cells containing nucleic acid sequences encoding the sFRP fragments and variants.07-21-2011
20090035819CA125 gene and its use for diagnostic and therapeutic interventions - The CA125 gene has been cloned and multiple repeat sequences as well as the carboxy terminus have been identified. The CA125 molecule comprises three major domains: an extracellular amino terminal domain (Domain 1); a large multiple repeat domain (Domain 2); and a carboxy terminal domain (Domain 3) which includes a transmembrane anchor with a short cytoplasmic domain. The amino terminal domain is assembled by combining five genomic exons, four very short amino terminal sequences and one extraordinarily large exon. This domain is dominated by its capacity for O-glycosylation and its resultant richness in serine and threonine residues. Additionally, an amino terminal extension is present, which comprises four genomic exons. The amino acid composition of the amino terminal extension was found to be consistent with the amino acid composition of the amino terminal domain. The molecular structure is dominated by a repeat domain comprising 156 amino acid repeat units, which encompass the epitope binding sites. More than 60 repeat units have been identified, sequenced, and contiguously placed in the CA125 domain structure. The repeat units encompass an interactive disulfide bridged C-enclosure and the site of OC125 and M11 binding. The repeat sequences demonstrated 70-85% homology to each other. Expression of the repeats was demonstrated in 02-05-2009
20080299608METHOD FOR THE IN VIVO MODIFICATION OF THE SYNTHESIS ACTIVITY OF A METABOLITE BY MEANS OF THE MODIFICATION OF A GENE THE ACTIVITY OF WHICH IS NOT THE ORIGINAL ACTIVITY - The invention relates to a method for altering a protein X such as to modify the characteristics thereof by a) obtaining the mutants X* of the sequence coding for protein X, by means of aleatory mutagenesis, b) transformation of cells with a phenotype [P-] with vectors comprising the mutated nucleic acids obtained in step (a) which code for proteins X*, where P-signifies that said cells are auxotrophic for substance P, P begin the product of the action of X on the natural substrate thereof S, c) culturing said cells in a medium comprising a substrate S*, S* being an analogue of the natural substrate S of the protein X, d) selection of the cells [P-:: X*] which have survived step c) in which the proteins X* can biosynthesise the product P from the substrate S*. The invention further relates to mutated proteins X, nucleic acids, expression vectors, host cells comprising a vector, use of N-dideoxyribosyl transferases for the transfer of a dideoxyribose (ddR) from a dideoxyribonucleoside to another nucleoside, a method for production of compounds comprising a step using a mutated protein and a strain of 12-04-2008
20120202248NOVEL HANSENULA POLYMORPHA GENE CODING FOR DOLICHYL-PHOSPHATE- MANNOSE DEPENDENT ALPHA-1,3 MANNOSYLTRANSFERASE AND PROCESS FOR THE PRODUCTION OF RECOMBINANT GLYCOPROTEINS WITH HANSENULA POLYMORPHA MUTANT STRAIN DEFICIENT IN THE SAME GENE - The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a 08-09-2012
20120202247METHOD, COMPUTING ROUTINE, DEVICE FOR PREDICTING PROPERTIES OF MHC/PEPTIDE COMPLEXES, AND DATA AND PEPTIDES PRODUCED THEREFROM - The present invention relates to a method for structure-based prediction of properties of peptides and peptide analogs in complex with major histocompatibility (MHC) class I and class II molecules. The said properties mainly relate to the three-dimensional structure of an MHC/peptide complex and the binding affinity of a peptide for an MHC receptor. The invention further relates to a computer program and a device therefor. The invention further relates to data produced by a method of the invention. The invention further relates to peptides and peptide analogs predicted to bind to target-MHC molecules. The present invention thus relates to the field of immunology, with possible applications in manufacture of vaccinates, de-immunization of proteins, and manufacture of therapeutic agents, especially immuno-therapeutic agents.08-09-2012
20100047865VECTOR FOR EXPRESSING NC PROTEIN OF HIV AND METHOD FOR PRODUCING NC PROTEIN USING THE SAME - The present invention relates to a vector for expressing an NC protein of HIV and a method for producing an NC protein using the same. More particularly, the present invention relates to a vector for expressing an NC protein of HIV, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, and a method for producing an NC protein using the same. The vector for expressing an NC protein of HIV of the present invention, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, can express a wild type NC protein in animal cells, and has an effect of improving the expression efficiency, as compared to a known art.02-25-2010
20090197301Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.08-06-2009
20100062485Novel Hansenula Polymorpha Gene Coding for Dolichyl-Phosphate- Mannose Dependent Alpha-1,3 Mannosyltransferase and Process for the Production of Recombinant Glycoproteins With Hansenula Polymorpha Mutant Strain Deficient in - The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a 03-11-2010
20080261269ALLELES OF THE OXYR GENE FROM CORYNEFORM BACTERIA - The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.10-23-2008
200802612685-Substituted Hydantoin Racemase, Dna Encoding the Same, Recombinant Dna, Transformed Cell, and Process for Production of Optically Active N-Carbamylamino Acid or Optically Active Amino Acid - Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from 10-23-2008
20080261267Cellulases from Rumen - The invention provides polypeptides coding for new cellulases from rumen, particularly from rumen ecosystem. The invention also relates to functional fragments or functional derivatives thereof as well as to nucleic acids encoding the polypeptides of the invention, vectors and host cells containing said nucleic acids, a method for producing the polypeptides and the use of the polypeptides according to the present invention for various industrial purposes and medical treatments.10-23-2008
20100068760PROTEINS WITH ESTERASE ACTIVITY - The invention relates to novel proteins having esterase activity, to mutants thereof, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.03-18-2010
20090162894NITRILE HYDRATASE - The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.06-25-2009
20090203072Detection for HPV-Induced invasive cancers and their precursor lesions with invasive potential - This invention provides a method of detecting an HPV-induced invasive cancer or precursor lesion thereof associated with tumor suppressor lung cancer 1 (TSLC1) in a subject in need thereof. The method comprises contacting a cell component of a test cell of the subject with a reagent that detects the level of the cell component in the test cell and determining a modification in the level of the cell component in the test cell as compared with a comparable healthy cell. The cell component indicates the level of TSLC1 in the cell, and a decrease in the level of TSLC1 indicates the presence of an HPV-induced invasive cancer or precursor lesion.08-13-2009
20120183995ENHANCED PROTEIN EXPRESSION IN BACILLUS - The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as 07-19-2012
20120183994Vector for Expression of hEGF and Uses Thereof - A vector for expression of hEGF that is made by constructing a gene sequence of SEQ NO: 1 on an expression vector for the transformation of 07-19-2012
20100009405Biosynthetic process for the preparation of gallidermin - A process for the production of gallidermin comprises fermentative production of the respective biologically in-active and non-toxic pre-form(s) by either genetically engineered producer organism or mutants of the natural producer organism that are deficient for the extracelluar gallidermin pathway specific serine-pro tease. The process includes a downstream process in which a pre-form of gallidermin is isolated from the fermentation and bio-catalytically activated, followed by purification of the mature and active gallidermin.01-14-2010
20100151519METHOD FOR PRODUCTION OF ISOPRENOIDS - The present invention is directed to variant squalene synthase enzymes, including 06-17-2010
20100151518VECTORS AND METHODS FOR ENZYME PRODUCTION - The invention provides a method for producing a replicase EF-Ts, EF-Tu and β polypeptide subunits, the method comprising expressing in a suitable host cell the EF-Ts and EF-Tu subunits, followed by expressing the β subunit, wherein the EF-Ts and EF-Tu subunits are operably linked to a first promoter and the β subunit is operably linked to a second promoter, and wherein the first and second promoters are differentially induced.06-17-2010
20100151517METHODS FOR CARRYING OUT THE SELECTIVE EVOLUTION OF PROTEINS IN VIVO - The present invention relates to methods for producing variants of proteins which have improved properties in comparison with the initial protein, the variants being obtained with the aid of an in vivo evolution method.06-17-2010
20100151516Gene SMS 13 - The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.06-17-2010
20110195451Protein Expression - An isolated DNA molecule having a sequence which comprises in a 5′ to 3′ direction (i) one or more promoter elements, (ii) the geneof interest, and (iii) a poly-adenylation 5 signal, and (iv) a terminator element, and expressing the geneof interest incorporated into the DNA molecule in an expression system, and use of said molecule to enhance expression of a gene of interest.08-11-2011
20100075376DNase Expression in Recombinant Host Cells - The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.03-25-2010
20090017494TICK OCTOPAMINE RECEPTOR NUCLEIC ACID MOLECULES, PROTEINS AND USES THEREOF - The present invention relates to tick octopamine receptor nucleic acid molecules; to tick octopamine receptor proteins encoded by such nucleic acid molecules; to antibodies raised against such proteins; and to compounds that inhibit the activity of such proteins. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. The present invention also includes inhibitory compounds, particularly those that specifically inhibit tick octopamine receptor activity, as well as the use of such compounds to treat animals.01-15-2009
20100120088MUTANT 6-PHOSPHOGLUCONATE DEHYDROGENASE - The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus 05-13-2010
20100120090MAMMALIAN EXPRESSION VECTOR pUHAB - The present invention relates to the construction and utilization of a new mammalian expression vector that contains a unique multiple cloning site (MCS), designated pUHAB. The pUHAB vector comprises a high copy replication origin (ColE1), a drug resistance gene (TK-Hygromycin), and a human cytomegalovirus promoter operably associated with a unique intron (hCMV/intron). Further, pUHAB comprises a selectable marker conferring resistance to kanamycin in bacterial cells, and a phage f1(+) region. pUHAB can be used to transiently or stably express cloned genes when transfected into mammalian cells. The invention also encompasses kits and host cells and cell lines comprising pUHAB, and methods of producing a recombinant protein using pUHAB.05-13-2010
20100120091SUBTILASE VARIANTS - The present invention relates to novel subtilase variants exhibiting improvements relative to the parent subtilase in one or more properties including: wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.05-13-2010
20100120089A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME - Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.05-13-2010
20100112639ENHANCED PRODUCTION AND PURIFICATION OF A NATURAL HIGH INTENSITY SWEETENER - Recombinant 05-06-2010
20100112636INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E COLI - An expression system for transforming 05-06-2010
20100075378MORAXELLA CATARRHALIS BASB115 POLYPEPTIDES - The invention provides BASB115 polypeptides and polynucleotides encoding BASB115 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses.03-25-2010
20100075377TETRAMERIZING POLYPEPTIDES AND METHODS OF USE - The present invention relates to a method of preparing a tetrameric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a vasodialator-stimulated phosphoprotein (VASP) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. The method can be used to produced homo- and hetero-tetrameric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.03-25-2010
20120244576COMPOSITIONS AND METHODS FOR IMPROVED PROTEIN PRODUCTION - The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 09-27-2012
20100041097Synthetic gene control region - The present invention provides a synthetic gene control region which comprises a gene regulatory sequence comprising a binding site for a gene regulatory protein of a yeast strain, and a promoter from filamentous fungal strain located downstream of the gene regulatory sequence; wherein the promoter can be recognized by the general transcription factors and RNA polymerase of the yeast strain; wherein the gene regulatory sequence is capable of regulating transcription initiated by the filamentous fungal promoter in the yeast strain.02-18-2010
20130078671INCORPORATION OF TWO DIFFERENT NONCANONICAL AMINO ACIDS INTO A SINGLE PROTEIN - The invention relates to methods, systems, and compositions for the genetic incorporation of a plurality of different noncanonical amino acids into one target protein. The invention provides for multiple, mutually orthogonal aminoacyl-tRNA synthetase/tRNA pairs that suppress two different selector codons engineered into a polynucleotide molecule. By virtue of the suppression of the selector codons, orthogonal aminoacyl-tRNA synthetase/tRNA pairs permit incorporation of their charged noncanonical amino acids into the corresponding positions in the protein. The noncanonical amino acids provide a wide array of functional capabilities. For example, the noncanonical amino acids can provide a reactive pair of moieties that facilitate the study and manipulation of the target protein.03-28-2013
20130078672POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.03-28-2013
20130078674Method for protein production in filamentous fungi - The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.03-28-2013
20130078673RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE - The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.03-28-2013
20090215116Identification of a beta-1,3-N-acetylgalactosaminyltransferase (CGTE) from campylobacter jejuni LIO87 - The invention relates to β-1,3-N-acetylgalactosaminyltransferase polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.08-27-2009
20100035301BETA-MANNANASE FROM COFFEE BERRY BORER, HYPOTHENEMUS HAMPEI, AND USES THEREOF - The present invention relates to an isolated β-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO:1, as well as isolated polynucleotides encoding the β-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing β-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, which involves providing plant material and contacting the plant material with the β-mannanase protein of the present invention under conditions effective to degrade mannans and polysaccharides in the plant material.02-11-2010
20130084604Method for improved protein production in filamentous fungi - The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.04-04-2013
20130084603B7L-1 POLYNUCLEOTIDES - The invention is directed to B7L-1 as a purified and isolated protein, the DNA encoding the B7L-1, host cells transfected with cDNAs encoding B7L-1 and processes for preparing B7L-1 polypeptides.04-04-2013
20100129867METHOD FOR PRODUCING DIPEPTIDE - The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and 05-27-2010
20090253173FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION - The invention relates to a filamentous fungal cell (e.g., 10-08-2009
20130034876Systems and Methods to Increase Protein Yield from Recombinant Manufacturing Processes - Embodiments disclosed herein provide systems and methods that increase protein yield from recombinant manufacturing processes. The systems and methods treat used depth filters with bound proteins of interest as a stationary phase exchange resin to recapture bound protein of interest from the depth filter.02-07-2013
20130034875DNA CONSTRUCT, AND PROCESS FOR PRODUCTION OF RECOMBINANT CHO CELL USING SAME - Disclosed is a DNA construct that is useful for efficient generation of recombinant CHO cells useful for the production of target proteins. The DNA construct is a construct comprising, from a 5′ end toward a 3′ end, a first homologous DNA fragment, a target protein gene, and a second homologous DNA fragment. The first and second homologous DNA fragments have homology allowing for homologous recombination with a part of a hypoxanthine-phosphoribosyltransferase enzyme (hprt) locus in a CHO cell genome and have a chain length of not less than 1 kbp.02-07-2013
20090093017Nucleic Acids Encoding Recombinant Protein A - Disclosed are new recombinant nucleic acids encoding protein A polypeptides and methods of using these nucleic acids.04-09-2009
20090035820Microbial Trypsin Mutants Having Chymotrypsin Activity And Nucleic Acids Encoding Same - The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.02-05-2009
20100124764SINGLE CHAIN ANTIBODY LIBRARY DESIGN - The invention provides polynucleotide vectors and linkers and methods for designing and making single chain variable fragment (“ScFv”) libraries. The invention also provides polynucleotide vectors and linkers and methods for reformatting the ScFv library into Fab and IgG formats for high throughput production and screening.05-20-2010
20090162895RIBULOSE, 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES - The present invention relates to novel ribulose-1,5-bisphosphate carboxylase/oxygenase polypeptides and the polynucleotides that encode them. The invention also provides related host cells and methods.06-25-2009
20090162893Alcohol dehydrogenase for the stereoselective production of hydroxy compounds - The invention relates to a DNA molecule encoding an NADP-dependent alcohol dehydrogenase, to a vector containing at least one copy of the DNA sequence, and to prokaryotic or eukaryotic host cells that are transformed or transfected with said DNA sequence. The invention also relates to the NADP-dependent alcohol dehydrogenase as such, to a method for the production and the use of the alcohol dehydrogenase and to a method for the stereoselective production of secondary alcohols.06-25-2009
20090155847Combinatorial DNA library for producing modified N-glycans in lower eukaryotes - The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man06-18-2009
20090155846KINASE ANCHOR PROTEIN MUTEINS, PEPTIDES THEREOF AND RELATED METHODS - A-kinase anchor protein (AKAPS) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein. Also provided are transgenic animals, cells comprising transgenes and various methods employing such peptides.06-18-2009
20090155844METHOD OF SYNTHESIZING A SUPPRESSOR tRNA, DNA CONSTRUCT AND USE THEREOF FOR PRODUCING A NON-NATURAL AMINO ACID-INCORPORATED PROTEIN - There are provided a DNA construct comprising non-eukaryote-derived suppressor tRNA gene containing no internal promoter functioning in a eukaryotic cell, and a eukaryote-derived or bacteriophage-derived promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing a non-natural amino acid-incorporated protein by using the same.06-18-2009
20090155843TNF ANTAGONISTS - TNF binding polypeptides based on human tetranectin C-type lectin like domains (CTLD) with improved binding characteristics and improved efficacy. The polypeptides comprise a TNF binding domain having the amino acid sequence KRWS-RYF (SEQ ID NO:1). Also provided are methods of preparing the polypeptides of the invention. The polypeptides may be used for the preparation of pharmaceutical compositions, and for treatment of a subject having a pathology mediated by TNF, such as treatment of rheumatoid arthritis.06-18-2009
20100105106PREPARATION AND USES OF GENE SEQUENCES ENCODING CHIMERICAL GLYCOSYLTRANSFERASES WITH OPTIMIZED GLYCOSYLATION ACTIVITY - The present invention relates to the production of gene sequences encoding chimerical membrane glycosyltransferases presenting an optimized glycosylation activity in cells transformed with said sequences, said gene sequences corresponding to the fusion: of a first nucleic acid coding for a C-terminal minimal fragment of the catalytic domain (CD) of the native full length glycosyltransferase, to a second nucleic acid coding for a transmembrane peptide comprising in its N-terminal region a cytoplasmic tail (CT) region located upstream from a transmembrane domain (TMD), itself located upstream of a stem region (SR), provided that at least one of these CT, TMD, SR peptides being different from the primary structure of the naturally occurring peptide counterparts present in the native glycosyltransferase from which is derived the CD fragment with optimal glycosyltransferase activity as defined above. The invention also relates to the use of said gene sequences in the frame of the preparation of recombinant proteins of interest by cells transformed with said sequences and sequences encoding said recombinant proteins.04-29-2010
20100105107PURIFICATION AND CHARACTERIZATION OF SOLUBLE MHC PROTEINS - The present invention relates generally to the production and use of functionally active soluble HLA molecules that are isolated and purified substantially away from other proteins, and methods of purifying same.04-29-2010
20100105108METHOD AND DEVICE FOR PRODUCING VACCINE - A method of making a vaccine using animal derived component free (ADCF) cell culture technology, including the steps of attaching ADCF-adapted cells to a microcarrier including an attachment mechanism for attaching filipodia of the cells, the microcarrier being in a culture, growing the cells in ADCF maintenance media, infecting the cells with vaccine media, producing virus within the cells, and harvesting the virus. A vaccine produced by the above method in a pharmaceutically acceptable carrier. A vaccine production structure of ADCF-adapted cells removably attached to microcarrier beads including an attachment mechanism for attaching filipodia of the cells.04-29-2010
20100041101EXPRESSION SYSTEM FOR RECOMBINANT HUMAN ARGINASE I - A novel recombinant protein expression system is provided for improving expression of recombinant human arginase I. The system contains an isolated and purified nucleic acid molecule for constructing plasmid and 02-18-2010
20100041100FAMILY 6 CELLULASE WITH DECREASED INACTIVATION BY LIGNIN - A modified 02-18-2010
20100041099CELLS EXPRESSING PICHIA CYTOCHROME C - Described herein are recombinant yeast cells that express a cytochrome C gene the expression of which causes the recombinant yeast cells to (i) grow faster than wild type yeast cells of the same species when cultured on glucose medium, and (ii) accumulate high levels of polyunsaturated fatty acid precursor molecules or target gene expression products when grown on oleic acid.02-18-2010
20100041098MODIFIED CLOSTRIDIAL TOXINS WITH ALTERED TARGETING CAPABILITIES FOR CLOSTRIDIAL TOXIN TARGET CELLS - The specification discloses modified Clostridial toxins comprising a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and an enhanced Clostridial toxin binding domain; polynucleotide molecules encoding such modified Clostridial toxins; and method of producing such modified Clostridial toxins.02-18-2010
20090061481HIGHLY-EFFICIENT HYPERTHERMOPHILIC DNA LIGASE - Disclosed is a modified hyperthermophilic DNA ligase having improved DNA binding ability and reactivity. The modified hyperthermophilic DNA ligase has an amino acid sequence corresponding to the amino acid sequence of a heat-resistant DNA ligase derived from a thermophilic bacterium, a hyperthermophilic bacterium, a thermophilic archaebacterium, or a hyperthermophilic archaebacterium, except with at least two of charged amino acids in the C-terminal helix region each being substituted by alanine, threonine, or serine residues.03-05-2009
20130029377RECOMBINANT APOA-1M FROM ENGINEERED BACTERIA - Apolipoprotein A-1 Milano (ApoA-1M), the protein component of a high-density lipoprotein (HDL) mimic with promising potential for reduction of atherosclerotic plaque, is produced on a large scale by expression in 01-31-2013
20100093031DNA ENCODING XYLITOL DEHYDROGENASE - The present invention relates to DNA encoding novel xylitol dehydrogenase and a method for using the same. Specifically, the present invention comprises providing the nucleic acid sequence of a xylitol dehydrogenase gene from 04-15-2010
20100093028MODIFIED VARIANT BOWMAN BIRK PROTEASE INHIBITORS - The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.04-15-2010
20090191589PROCESS FOR PRODUCING DIPEPTIDES - The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.07-30-2009
20120164685POLYPEPTIDES AND IMMUNIZING COMPOSITIONS CONTAINING GRAM POSITIVE POLYPEPTIDES AND METHODS OF USE - The present invention provides isolated polypeptides isolatable from a 06-28-2012
20100009406Nucleic acids encoding a heme binding protein polypeptide - The present invention relates to methods, reagents and kits for detecting of formyl peptide receptor like-2 (FPRL2) polypeptide activity in a sample and identifying agents which modulate polypeptide activity. It further relates to antibodies raised against FPRL2. It further relates to substances for preventing, treating and/or alleviating diseases or disorders characterized by dysregulation of FPRL2 polypeptide signalling.01-14-2010
20090104658Heterologous Production of Capreomycin and Generation of New Capreomycin Derivatives Through Metabolic Engineering - Provided are nucleic acid molecules comprising at least a functional fragment of the capreomycin biosynthetic gene cluster, polypeptides encoded by the cluster and recombinant host cells transformed with any of the nucleic acid molecules disclosed herein. Various methods using any of the vectors or expression cassettes that encode one or more of the gene products of the cluster are provided for heterologous production of capreomycin and capreomycin derivatives.04-23-2009
20100068763MAMMALIAN CHEMOKINES; RECEPTORS; REAGENTS; USES - Novel chemokines and 7 transmembrane receptors from mammals, reagents related thereto, including purified proteins, specific antibodies, and nucleic acids encoding the chemokines and receptors are disclosed. Methods of using the chemokines, receptors, reagents and diagnostic kits are also provided.03-18-2010
20090123968Compositions Containing, Methods Involving, and Uses of Non-Natural Amino Acids and Polypeptides - Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.05-14-2009
20090123967Modified spider silk proteins - The present invention is directed to a method of modifying a spider silk protein and a spider silk protein obtainable by said method. The invention further pertains to a nucleic acid sequence coding for a modified spider silk protein, a vector containing said sequences and host cells transformed with this vector. The invention furthermore is directed to a pharmaceutical or cosmetical composition containing a modified spider silk protein as defined herein and the use of said modified sequences in various fields, in particular in the fields of medicine, cosmetics and technical applications.05-14-2009
20090123970GLYCOSYLATED MAMMALIAN NGAL AND USE THEREOF - The present invention relates to glycosylated mammalian NGAL, and methods of using said glycosylated mammalian NGAL.05-14-2009
20090123969NOVEL FLUORESCENT PROTEIN AND GENE ENCODING THE SAME - The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO: 1 and it is a fluorescent protein derived from a copepod taxonomically classified to 05-14-2009
20130052683PRODUCTION OF GALACTOSYLATED N-GLYCANS IN PLANTS - The invention provides methods for increasing the levels of bi-antennary mono- and fully galactosylated N-glycans, and for decreasing the levels of hybrid-type galactosylated N-glycans on glycoproteins produced in plants or plant cells. In addition, the invention provides methods for the production of heterologous glycoproteins with increased levels of bi-antennary mono- and fully galactosylated N-glycans, or decreased levels of hybrid-type galactosylated N-glycans in plants or plant cells.02-28-2013
20130052684METHOD FOR PRODUCING USEFUL SUBSTANCES BY A RECOMBINANT ACTINOMYCETE, STREPTOMYCES SPECIES - The invention provides a promoter derived from a genome of an actinomycete, 02-28-2013
20100136623MUTATIONS IN ION CHANNELS - A method of identifying a subject predisposed to a disorder associated with ion channel dysfunction, comprising ascertaining whether at least one of the genes encoding ion channel subunits in said subject has undergone a mutation event such that a cDNA derived from said subject has the sequence set forth in one of SEQ ID NOS: 1-134.06-03-2010
20090325229Process for Globular Adiponectin Production - This invention relates to methods for use in industrial production of recombinant globular Adiponectin (gAdiponectin). Specifically, the present invention provides a purification process suitable for production of high amounts of pure gAdiponectin. gAdiponectin is expressed in 12-31-2009
20090317863USE OF ACTIVE CYTOKININ SYNTHASE GENE - It is an object of the present invention to clarify an enzyme gene capable of directly regulating the amount of active cytokinins synthesized and to provide a transformed plant with a regulated amount of cytokinins using the aforementioned gene.12-24-2009
20090317862Cell-free protein synthesis system for synthesizing glycoprotein - Provided is a process for producing a protein with post-translational modification in a cell-free protein synthesis system having a higher protein synthetic activity and glycosylation capability. The process of the present invention comprises preparing a cell extract from cultured cells of an immortalized mammalian cell line that has enhanced protein secreting activity, and adding an mRNA encoding a glycoprotein to the cell extract.12-24-2009
20090311744EXPRESSION OF O-GLYCOSYLATED THERAPEUTIC PROTEINS IN PROKARYOTIC MICROORGANISMS - The invention relates to methods of producing an O-glycosylated soluble therapeutic protein in a prokaryotic microorganism by co-expressing the therapeutic protein and a heterologous glycosyltransferase that transfers a sugar moiety to an amino acid acceptor on the therapeutic protein.12-17-2009
20090311742Phage-Derived Vectors and Methods for Protein Expression - A novel system, including vectors, 12-17-2009
20090191588VECTORS WITH MODIFIED INITIATION CODON FOR THE TRANSLATION OF AAV-REP78 USEFUL FOR PRODUCTION OF AAV - The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.07-30-2009
20100267088INHA PROMOTERS - The present invention relates to an isolated DNA sequence having promoter activity and to vectors comprising the DNA sequence. The promoters of the invention are referred to inhA promoters. The invention also relates to a method of producing a protein in a recombinant host cell wherein the host cell comprises a vector comprising an inhA promoter and a coding sequence encoding a protein of interest.10-21-2010
20090042246Methods For The Production And Secretion Of Modified Peptides - The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.02-12-2009
20090042245Methods of overexpression and recovery of porcine circovirus type 2 ORF2 - An improved method for recovering the protein expressed by open reading frame 2 from PCV2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.02-12-2009
20090042249Vectors for Cloning - A vector for transformation into a host cell is described comprising a toxic gene encoding a product that is lethal to the host cell, wherein the toxic gene comprises: 02-12-2009
20130089894Methods For Increasing Homologous Recombination Of A Nucleic Acid Sequence - The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.04-11-2013
20130089893MULTIMERIC Fc RECEPTOR POLYPEPTIDES - A soluble multimeric protein or polypeptide is disclosed that is able to inhibit interaction of leukocyte Fcγ receptors (FcγR) and immunoglobulin G (IgG). The protein or polypeptide comprises two or more linked Fc binding regions, at least one of which is derived from an FcγR type receptor and, particularly, FcγRIIa. Also described are polynucleotide molecules encoding the protein or polypeptide and the use thereof in methods of treating a subject for an immune-complex (IC)-mediated inflammatory disease.04-11-2013
20090029415Peripheral nervous system specific sodium channels, DNA encoding therefor, crystallization, X-ray diffraction, computer molecular modeling, rational drug design, drug screening, and methods of making and using thereof - Cloning, expression, viral and delivery vectors and hosts which contain nucleic acid coding for at least one peripheral nervous system specific (PNS) sodium channel peptide (SCP), isolated PNS SCP, and compounds and compositions and methods, are provided, for isolating, crystallizing, x-ray analysing molecular modeling, rational drug designing, selecting, making and using therapeutic or diagnostic agents or ligands having at least one peripheral nervous system specific (PNS) sodium channel (SC) modulating activity.01-29-2009
20110003333Method for Increasing Expression Yield of a Protein of Interest - The present invention relates to a method of producing a protein of interest comprising: (a) cultivating a mutant cell under conditions conducive for production of the protein wherein the mutant has a reduced or no expression of an endogenous polypeptide shown in SEQ ID NO: 2 compared to a parent host cell grown under the same conditions; and optionally (b) recovering the protein of interest.01-06-2011
20090305347Pichia Pastoris P1R1 Secretion Signal Peptide for Recombinant Protein Expression and Pichia Pastoris P1R1 and P1R2 Anchor Domain Peptides for Recombinant Surface Display - The present invention is directed to recombinant nucleic acid constructs comprising nucleic acid sequences encoding a 12-10-2009
20090305348DIAGNOSTIC TEST FOR VITAMIN B12 - An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and SEQ ID NO: 9. The invention also encompasses an isolated nucleic acid sequence or fragment thereof comprising, or complementary to, a nucleotide sequence having at least 85% nucleotide sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, and SEQ ID NO: 7. The porcine intrinsic factor can be use is an assay to determine the quantity of vitamin B12-10-2009
20090305346Engineered cleavage half-domains - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.12-10-2009
20090305345POLYMERASE - The present invention relates to an engineered polymerase characterized in that the polymerase exhibits an enhanced ability to process nucleic acid in the presence of environmental and biological inhibitors compared to wild type DNA polymerase.12-10-2009
20090305344CHIMERIC ALPHAVIRUS REPLICON PARTICLES - Chimeric alphaviruses and alphavirus replicon particles are provided, including methods of making and using same. Specifically, alphavirus particles are provided having nucleic acid molecules derived from one or more alphaviruses and structural proteins (capsid and/or envelope) from at least two or more alphaviruses. Methods of making, using, and therapeutic preparations containing the chimeric alphavirus particle, are disclosed.12-10-2009
20090305343Method For Expressing Polypeptides In Eukaryotic Cells Using Alternative Splicing - This invention relates to an expression cassette for expressing polypeptides in eukaryotic cells using alternative splicing. The expression cassette comprises in 5′ to 3′ downstream direction: a promoter; a sequence transcribed in a 5′ untranslated region (5′UTR); a donor splice site; an intron; a first acceptor splice site; a first cistron encoding a first polypeptide; a second acceptor splice site; a second cistron encoding a second polypeptide; an internal ribosome entry site (IRES) operably linked to a selection marker; and a sequence transcribed in a 3″ untranslated region (3′UTR) including a polyadenylation signal, wherein the polyadenylation signal is unique.12-10-2009
20090305342Solubilization and Purification of a Target Protein fused to a Mutant Maltose-Binding Protein - Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.12-10-2009
20090305341HCL-K1 Polypeptide Which Offers Collectin Activity - Disclosed is the novel hCL-K1 polypeptide which offer collectin activity. This polypeptide consists of consecutive 271 amino acids set out in SEQ ID NO: 2 and does not bind to both maltose and N-acetylgalactosamine.12-10-2009
20090305340ALTERED PEPTIDE LIGANDS OF GAD65 - Modified GAD65 compositions antagonize the activities of islet-specific T cells that contribute to the progression of one or more autoimmune disorders. The compositions are also antagonistic in humanized mice that express human HLA alleles associated with increased-risk of Type 1 diabetes.12-10-2009
20090305339Polypeptides With Laccase Activity - The invention relates to a new laccase from rumen, namely the RL5 laccase. In particular, the invention relates to a polypeptide comprising the amino acid sequence shown in 12-10-2009
20130071878TWO HELIX BINDERS - Provided herein are isolated polypeptides derived from the staphylococcal protein A protein B domain comprising a pair of anti-parallel alpha helices that are capable of binding a target. Also provided are nucleic acid sequences encoding such two helix binders, vectors containing the nucleic acid sequences encoding for two helix binders, and host cells transformed with vectors containing the nucleic acid sequences encoding for the two-helix binders. Also provided are methods of using the two helix binders.03-21-2013
20090061482NUCLEOTIDE SEQUENCES OF CORYNEFORM BACTERIA CODED FOR PROTEINS PARTICIPATING IN L-SERINE METABOLISM AND METHOD FOR MICROBIAL PRODUCTION OF L-SERINE - This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.03-05-2009
20090093022Chemosensory gene family encoding gustatory and odorant receptors and uses thereof - This invention provides an isolated nucleic acid encoding an insect gustatory or odorant receptor. This invention provides a nucleic acid of at least 12 nucleotides capable of specifically hybridizing with a nucleic acid encoding an insect gustatory or odorant receptor. This invention also provides a purified, insect gustatory or odorant receptor. This invention provides an antibody capable of specifically binding to an insect gustatory or odorant receptor. This invention provides a method of identifying a compound capable of specifically binding to, activating, or inhibiting the activity of an insect gustatory or odorant receptor. This invention also provides methods of controlling insect populations.04-09-2009
20090093021LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING CARBOHYDRATE UTILIZATION-RELATED PROTEINS AND USES THEREFOR - Carbohydrate utilization-related and multidrug transporter nucleic acids and polypeptides, and fragments and variants thereof, are disclosed in the current invention. In addition, carbohydrate utilization-related and multidrug transporter fusion proteins, antigenic peptides, and anti-carbohydrate utilization-related and anti-multidrug transporter antibodies are encompassed. The invention also provides vectors containing a nucleic acid of the invention and cells into which the vector has been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.04-09-2009
20090093020Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins - The present invention provides methods to reduce or eliminate α-mannosidase resistant glycans on glycoproteins in yeast. The reduction or elimination of α-mannosidase resistant glycans on glycoproteins results from the disruption of the newly isolated 04-09-2009
20090093019PRODUCTION AND IN VIVO ASSEMBLY OF SOLUBLE RECOMBINANT ICOSAHEDRAL VIRUS-LIKE PARTICLES - The present invention provides an improved method for the in vivo production of soluble assembled virus-like particles (“VLPs”) in bacterial cells of Pseudomonad origin. The Pseudomonad cells support assembly of VLPs from icosahedral viral capsid proteins (“CPs”) in vivo, and allow the inclusion of larger recombinant peptides as monomers or concatamers in the VLP. The invention specifically provides an improved method for the in vivo production of soluble assembled Cowpea Chlorotic Mottle Virus (“CCMV”) VLPs by introducing modifications into the CCMV CP that result in high yield production of soluble CP fusions in a 04-09-2009
20120309052INDUCIBLE BACULOVIRUS SYSTEM IN INSECT CELLS - The present invention describes a novel baculovirus protein expression system in insect cells, where the expression of recombinant protein(s) is inducible. By the inducible expression systems of the invention, expression of the recombinant protein of interest can be repressed during virus amplification, and thereafter activated in the presence or absence, respectively, of an inducing molecule or by a change in environmental conditions. The novel expression system does not produce significant levels of recombinant protein by baculovirus-infected cells during virus amplification prior to induction, thereby reducing selection pressure on the expression cassette for the recombinant protein. When induction is initiated, an increased yield of recombinant protein is thus produced relative to presently available, non-inducible baculovirus expression methods. Further, it is demonstrated herein that the yield of protein expression derived from the described methodology further increases relative to non-inducible systems as the scale of protein expression increases.12-06-2012
20110014651METHOD FOR HIGH-LEVEL SECRETORY PRODUCTION OF PROTEIN - This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.01-20-2011
20110014650IN VIVO UNNATURAL AMINO ACID EXPRESSION IN THE METHYLOTROPHIC YEAST PICHIA PASTORIS - The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as 01-20-2011
20110014649METHOD FOR PRODUCTION OF N36-BINDING PEPTIDE - The present invention relates to the production of an N36-binding peptide at low cost and in a large quantity utilizing a microorganism. More specifically, the present invention relates to a method for producing an N36-binding peptide comprising introducing a recombinant vector into which a DNA molecule encoding an N36-binding peptide that binds to an N36 protein derived from a retrovirus that causes immunodeficiency in a mammal has been incorporated into 01-20-2011
20130059336HYDROPHOBIC INTERACTION CHROMATOGRAPHY METHOD - Herein is reported a method for purifying a polypeptide comprising a histidine-tag comprising the steps of i) applying a solution comprising the polypeptide with a histidine-tag to a hydrophobic interaction chromatography material, and ii) recovering the polypeptide comprising a histidine-tag with a solution comprising imidazole or an imidazole-derivative and thereby purifying the polypeptide comprising a histidine-tag, wherein the solution comprising the polypeptide applied to the hydrophobic interaction chromatography material is free of imidazole or an imidazole-derivative and the polypeptide adsorbed to the hydrophobic interaction chromatography material is recovered with a solution comprising imidazole or an imidazole-derivative.03-07-2013
20130059335USE OF A GENETICALLY MODIFIED CELL LINE EXPRESSING FUNCTIONAL ASIALOGLYCOPROTEIN RECEPTOR IN THE PRODUCTION OF SIALYLATED GLYCOPROTEINS - The present invention is directed to the use of a cell line in the production of sialylated glycoprotein, wherein said cell line expresses functional ASGPR protein as well as to a method for the production of sialylated glycoproteins, characterized in that such a cell line is used.03-07-2013
20130059334PRODUCTION OF RECOMBINANT SELENOPROTEIN MUTANTS WITH ENHANCED CATALYTIC ACTIVITY - The present invention generally relates to the production of industrially relevant quantities of selenoprotein enzymes in eukaryotic cell cultures. More specifically, the present invention generally relates to the production of such enzymes wherein one or more catalytic cysteine or serine residues are mutagenically replaced by selenocysteine.03-07-2013
20120190066 HOST CELL FOR THE PRODUCTION OF A COMPOUND OF INTEREST - The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell. The invention further relates to the production of a compound of interest. The invention further relates to isolated polynucleotides and vectors and host cells comprising said polynucleotides.07-26-2012
20090298120METHOD FOR EXPRESSING SIALYLATED GLYCOPROTEINS IN MAMMALIAN CELLS AND CELLS THEREOF - Methods and systems for producing glycoproteins having sialylated oligosaccharides are provided. The invention comprises the genetic engineered cells with a CMP-sialic acid transporter (CMP-SAT) gene so that the cells express the CMP-SAT protein or fragment thereof at an above endogenous levels. The increase in CMP-SAT expression allows for the increased transport of the CMP-sialic acid into the Golgi apparatus so as to obtain sialylation of glycoproteins at above endogenous levels. In particular, the methods and systems of the invention are useful for producing complex sialylated glycoproteins in mammalian cells of interest, for example Chinese Hamster Ovary (CHO) cells.12-03-2009
20090280530NOVEL COLLAGEN-LIKE PROTEIN CLAC, PRECURSOR THEREOF AND GENES ENCODING THE SAME - A novel human collagen-like protein CLAC occurring in brain amyloid and its precursor CLAC-P; genes encoding the same; cDNA of mouse CLAC-P and its deduced amino acid sequence; antibodies specific to these proteins; and methods of diagnosing treating and preventing Alzheimer's disease by using the same.11-12-2009
20090269809THERMOSTABLE RIBONUCLEASE H - A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.10-29-2009
20090269808VIRUS COAT PROTEIN VARIANTS WITH SELF-SUBTRACTING PROPERTIES - Herein is described a modified viral vector comprising: a coat protein modified, for example by the addition of a cysteine residue, such that the modified viral vector yields less soluble virus relative to that from an unmodified viral vector upon extraction of plant material infected with the modified viral vector, thereby facilitating purification of a recombinant protein expressed from the modified viral vector. Also described is a method of reducing viral coat protein impurities during purification of a recombinant protein, a method of biocontainment for a recombinant viral vector, and a method of generating virus inoculum for the modified viral vector.10-29-2009
20090269807Compositions and Methods for the Expression of Selenoproteins in Eukaryotic Cells - Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.10-29-2009
20090269806PROCESS FOR PRODUCING DIPEPTIDES - The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least one of said one or more kinds of amino acids, allowing the dipeptide to form and accumulate in the medium, and recovering the dipeptide from the medium.10-29-2009
20090269805Novel Lipolytic Enzyme ELIP - The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.10-29-2009
20090269804Methods for refolding proteins containing free cysteine residues - The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.10-29-2009
20090269803In Vivo Generation of Dna, Rna, Peptide, and Protein Libraries - The present invention relates to a method for the in vivo generation of DNA, RNA, peptide and protein libraries by means of a genetic element harboring a viral or phage origin of replication that is independently reproduced by a viral or phage error-prone polymerase not physically linked to the genetic element within a host cell furthermore containing viral or phage auxiliary nucleotide sequences and proteins that are required for replication of the viral or phage genetic element. The nucleotide or nucleotides of interest to be diversified are introduced into the genetic element and physically linked to the viral or phage origin of replication.10-29-2009
20090269802ALPHA 1,4-GALACTOSYLTRANSFERASE AND DNA ENCODING THEREOF - The object of the present invention is to provide α1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme.10-29-2009
20090269801Vector to Induce Expression of Recombinant Proteins under Anoxic or Microaerobic Conditions - A protein expression vector that expresses large quantities of recombinant proteins under anoxic or microaerobic conditions by inducing expression with nitrate. The vector backbone is pUC19 and protein expression is driven by the 10-29-2009
20130065275SERUM-FREE MAMMALIAN CELL CULTURE MEDIUM, AND USES THEREOF - The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.03-14-2013
20130065274Recombinant Prokaryotes and Use Thereof for Production of O-Glycosylated Proteins - The present invention embraces a recombinant prokaryotic host cell containing nucleic acids encoding an eukaryotic UDP-GaINAc:UDP-GaINAc polypeptide transferase and expressing an UDP-GIcNAc C-4 epimerase and methods for using the same to produce an O-glycosylated protein.03-14-2013
20110020869METHODS FOR ALTERING ACETIC ACID PRODUCTION AND ENHANCING CELL DEATH IN BACTERIA - Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.01-27-2011
20110020865Gene Expression Technique - The present invention provides a host cell suitable for enhanced production of a protein product of choice characterised in that the host cell is genetically modified to cause over-expression of two or more helper proteins selected from a DnaJ-like protein (such as JEM1), an Hsp70 family protein (such as LHS1) and SIL1 wherein at least one of the over-expressed two or more helper proteins is selected from JEM1, LHS1 and SIL1, and wherein the DnaJ-like protein is not SCJ1.01-27-2011
20110045532PROTEIN PRODUCTION METHOD - This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.02-24-2011
20090233332METHOD OF PRODUCING RECOMBINANT PROTEIN - An object of the present invention is to provide a method of mass-producing recombinant protein by the liquid culture method with koji molds as host. According to the present invention, it is provided a method of producing recombinant protein by using the recombinant koji molds which are obtained by transforming koji molds as host comprising: culturing the recombinant koji molds in a liquid medium which contains as culture raw material at least one selected from the group consisting of the cereal of which surface is entirely or partly covered with at least husks, the bean and/or the tuber of which surface is covered with hulls and the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing; and collecting the recombinant protein from the culture product.09-17-2009
20090047709NOVEL ENDORIBONUCLEASE - A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA.02-19-2009
20090047710ST3Gal-1/ST6GalNAc-1 Chimeras - The present invention features compositions and methods related to increasing the solubility and enzymatic activity of GalNAc-α-2,6-sialyltransferase I (STÌGalNAcI) proteins expressed in prokaryotic host cells. Methods for increasing the solubility of STÌGalNAcI polypeptides include modifying cysteine residues, modifying N-linked glycosylation sites, deleting polypeptide regions, and constructing chimeric polypeptides comprising sequences from a STÌGalNAcI and another protein, for example, a Gal-β-1,3GalNAc-α-2,3-sialyltransferase (ST3GalI) and a STÌGalNAcI. The invention also features nucleic acids encoding such improved polypeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.02-19-2009
20090047708HIGHLY PRODUCTIVE RECOMBINANT YEAST STRAINS WITH MODIFIED GALACTOSE-REGULATED TRANSCRIPTION - A recombinant yeast cell with modified galactose-regulated transcription, a use of such yeast cell, and products made using such yeast cell are disclosed. In such a recombinant yeast cell, a chromosomal gene encoding an enzyme for transformation of a transcription inductor into an inactive product is replaced by a gene encoding a transcription factor for a galactose-inducible promoter. Examples of yeast cells include those from Saccharomycetaceae or Cryptococcaceae families or from the 02-19-2009
20090047706PROCESS FOR PRODUCING DIPEPTIDES - The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.02-19-2009
20090325231THERMOSTABLE L-RIBOSE ISOMERASE AND METHOD FOR PRODUCING SAME AND USE OF SAME - Object: To provide a thermostable L-ribose isomerase.12-31-2009
20090029417Recombinant Microorganism - The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a 01-29-2009
20090029416HYPERTHERMOSTABLE PROTEASE GENE - There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.01-29-2009
20090011462Polypeptides having Lipase Activity and Polynucleotides Encoding Same - The present invention relates to polypeptide having lipase activity and which further has a RP of at least 0.8 and a BR of at least 1.1 at the test conditions given in the specification.01-08-2009
20090011461Human Spasmolytic Polypeptide in Glycosylated Form - Human spasmolytic polypeptide (HSP) which has the amino acid sequence Glu Lys Pro Ser Pro Cys Gln Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly Ile Thr Ser Asp Gln Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gln Glu Ser Asp Gln Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly Ile Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe Ile Phe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu Asp Cys His Tyr01-08-2009
20090011460Calcium-binding photoprotein, gene encoding the same, and use thereof - A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.01-08-2009
20090203069Organic compounds - Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.08-13-2009
20080311622ACTIVATABLE RECOMBINANT NEUROTOXINS - Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids.12-18-2008
20080311619Recombinant Carboxypeptidase B - A nucleic acid coding for pro-carboxypeptidase B (Pro-CPB), comprising three segments A, B and C, wherein at least one of the segments has one of the sequences according to SEQ ID No. 1, 2 or 3.12-18-2008
20080280323Method for Producing Epidermal Growth Factor Using Fusion Proteins Comprising Fas-1 Domain - The present invention provides a fusion protein in which a polypeptide comprising Fas-1 domain is fused in frame to N-terminal or C-terminal of human EGF, a nucleotide sequence encoding the fusion protein, an expression vector containing the nucleotide sequence, a transformant transformed by the nucleotide sequence and a method for producing human EGF, improving stability of the protein and enhancing the functions of the same. The present invention provides human EGF with improved stability and enhanced functions by fusing a polypeptide comprising Fas-1 domain having the activities of cell adhesion and wound healing to human EGF.11-13-2008
20120237973PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION - A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more α-1,2-mannosidases.09-20-2012
20120237972MAMMALIAN-TYPE GLYCOSYLATION IN PLANTS - The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising a functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.09-20-2012
20080274503Modified Shine-Dalgarno Sequences and Methods of Use Thereof - Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.11-06-2008
20090136996Novel cysteine-depleted hydrophobin fusion proteins, their production and use thereof - Polypeptides of the general structural formula (I)05-28-2009
20090148896Hyperthermophilic DNA Polymerase and Methods of Preparation Thereof - The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a 06-11-2009
20120107871METHOD OF PRODUCING HUMAN IGG ANTIBODIES WITH ENHANCED EFFECTOR FUNCTIONS - A method for generating human IgG05-03-2012
20100093030PRODUCTION OF SECRETED PROTEINS BY FILAMENTOUS FUNGI - The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.04-15-2010
20080268500Novel Sesquiterpene Synthases and Methods of their Use - The present invention relates to novel terpene synthases. The terpene synthases are capable of synthesising mono-, bi- and/or tri-cyclic sesquiterpenes having a C2-C7 or a C3-C7 bond, starting from an acyclic pyrophosphate terpene precursor, farnesyl-pyrophosphate. Accordingly, for the first time, sesquiterpene synthases catalyzing the cyclisation to the santalene and bergamotene carbon skeleton are disclosed. The present invention further relates to nucleic acid sequences encoding the sesquiterpene synthases and to methods for making terpenoids.10-30-2008
20100068761ISOLATION AND CHARACTERIZATION OF A NOVEL PYTHIUM OMEGA 3 DESATURASE WITH SPECIFICITY TO ALL OMEGA 6 FATTY ACIDS LONGER THAN 18 CARBON CHAINS - The present invention relates to a polynucleotide encoding an omega 3 (ω-3) desaturase from 03-18-2010
20110281299Recombinant Pokeweed Antiviral Proteins, Compositions and Methods Related Thereto - The present invention provides novel, modified pokeweed antiviral proteins, nucleic acids that encode the proteins, conjugates that incorporate the proteins, and methods to make and use the proteins. The present invention also provides methods to administer the conjugates to animals, for the purpose of directing toxin to particular cells.11-17-2011
20100273213METHOD FOR EXPRESSION OF SPECIFIC GENE - Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.10-28-2010
20100028944Methods of obtaining genetic competence in bacillus cells - The present invention relates to methods of obtaining genetic competence in non-competent 02-04-2010
20090053762Cell/tissue culturing device, system and method - A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.02-26-2009
20090170160Expression System - A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.07-02-2009
20100267085NOVEL PSEUDOCHLOROCOCCUM SPECIES AND USES THEREFOR - The present invention relates to algal species and compositions, methods for identifying algae that produce high lipid content and possess CO10-21-2010
20090130714Process for purifying recombinanat tissue plasminogen activator (TPA) - The present invention relates to an efficient and improved process for purifying a recombinant protein. The invention relates to the purification of tissue plasminogen activator (tPA), such as truncated human tPA, recombinantly produced in bacteria, for example in 05-21-2009
20090029418METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI - The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.01-29-2009
20090035817Method for Preparing Transformants Expressing Benzaldehyde Dehydrogenase and Preparation of 2,6-Naphthalene Dicarboxylic Acid Using the Transformants - Disclosed herein are a method for preparing a transformant which carries a gene encoding benzaldehyde dehydrogenase derived from 02-05-2009
20100129865KETOSE 3-EPIMERASE, ITS PREPARATION AND USES - An object of the present invention is to provide a novel ketose 3-epimerase, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for producing a ketose by using the enzyme. The present invention solves the above objects by providing a ketose 3-epimerase which is obtainable from a microorganism of the genus 05-27-2010
20090311749Method for methanol independent induction from methanol inducible promoters in Pichia - The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.12-17-2009
20090087880PROCESS AND GENES FOR EXPRESSION ANDOVEREXPRESSION OF ACTIVE [FeFe] HYDROGENASES - A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.04-02-2009
20090042250Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression - Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.02-12-2009
20100221775INCREASED PRODUCTION OF SECRETED PROTEINS BY RECOMBINANT EUKARYOTIC CELLS - Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.09-02-2010
20110300576T7 EXPRESSION SYSTEM - The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.12-08-2011
20090325230PROTEIN EXPRESSION SYSTEMS - The present invention provides an improved expression system for the production of recombinant polypeptides utilizing auxotrophic selectable markers. In addition, the present invention provides improved recombinant protein production in host cells through the improved regulation of expression.12-31-2009
20110287480NEUROGENIN - The invention relates to novel neurogenin proteins, nucleic acids and antibodies.11-24-2011
20100062487Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic, and other uses - The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods using compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes.03-11-2010
20090191587METHOD FOR HIGH-LEVEL SECRETORY PRODUCTION OF PROTEIN - This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.07-30-2009
20120107872THERMOSTABLE TRICHODERMA CELLULASE - Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and 05-03-2012
20100196953CLEAVAGE OF PRECURSORS OF INSULINS BY A VARIANT OF TRYPSIN - The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin.08-05-2010
20110287482MEDIUM FOR THE PROTEIN-FREE AND SERUM-FREE CULTIVATION OF CELLS - A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.11-24-2011
20110287481HOST, TRANSFORMANT AND METHOD FOR PRODUCING THE TRANSFORMANT AND METHOD FOR PRODUCING O-GLYCOSYLATED HETEROLOGOUS PROTEIN - The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by using 11-24-2011
20090280531Preparation of Soluble Capsid Proteins of Picornaviruses Using SUMO Fusion Technology - A method of producing a soluble capsid protein of a picornavirus using a novel and efficient SUMO fusion protein expression system.11-12-2009
20090181424Mammalian Expression Vector Comprising the MCMV Promoter and First Intron of HCMV Major Immediate Early Gene - A mammalian expression vector that is a murine CMV promoter and the first intron of the major immediate early gene of the human cytomegalovirus. There are mammalian host cells containing the expression vector. There is also a process for the production of recombinant protein by using the expression vector.07-16-2009
20080274501METHOD OF PURIFYING ACIDIC PROTEINS EXPRESSED IN PLANTS - The present invention provides a rapid and relatively simple process for purification of acidic proteins expressed in tobacco cells. The process comprises three main purification steps: precipitation with polyethyleneimine, column chromatography with a hydrophobic interaction resin, and column chromatography with hydroxyapatite. The process provides pure or essentially pure protein at a very high yield.11-06-2008
20120015400HCL-K1 Polypeptide Which Offers Collectin Activity - Disclosed is the novel hCL-K1 polypeptide which offers collectin activity. This polypeptide consists of consecutive 271 amino acids set out in SEQ ID NO: 2 and does not bind to both maltose and N-acetylgalactosamine.01-19-2012
20080206815Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.08-28-2008
20110294160Cysteine Protease Autoprocessing of Fusion Proteins - Disclosed are fusion proteins, polynucleotides that encode the disclosed fusion proteins, and methods for expressing and autoprocessing of the disclosed fusion proteins to obtain a target protein. The disclosed fusion proteins include an autoproteolytic cysteine protease fused to a heterologous polypeptide, which may be isolated as the target protein. Preferably, the protease activity of the cysteine protease is inducible. Suitable autoproteolytic cysteine proteases for the fusion proteins include the cysteine protease of the 12-01-2011
20090311747VECTORS AND METHODS FOR HIGH THROUGHPUT CO-EXPRESSIONS - The present invention includes vectors and methods for high throughput co-expression.12-17-2009
20130189733PRODUCTION OF SECRETED PROTEINS BY FILAMENTOUS FUNGI - The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.07-25-2013
20110020867Constructs And Methods For Efficient Transformation Of Micro-Organisms For Production Of Carbon-Based Products Of Interest - Improved constructs for increasing efficiency of transformation of thermophilic host cells for production of carbon-based products of interest and methods for producing carbon-based products of interest are provided.01-27-2011
20090298125HALOHYDRIN DEHALOGENASES AND RELATED POLYNUCLEOTIDES - The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.12-03-2009
20090298124Site-specific incorporation of redox active amino acids into proteins - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.12-03-2009
20090298123Novel Lipase Genes - New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.12-03-2009
20080268501METHODS AND MATERIALS RELATING TO GENE EXPRESSION - An expression cassette for expressing a nucleic acid of interest derived from the regulatory region of the methylenomycin gene cluster of the SCP1 plasmid of 10-30-2008
20080293101Engineered microorganisms for increasing product yield in biotransformations, related methods and systems - There are disclosed recombinant microorganisms engineered to increase product yield in a biotransformation. In an embodiment, the microorganisms are engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. There are also disclosed methods and systems for using recombinant microorganisms engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. Other embodiments are also disclosed.11-27-2008
20100099146Variants of Beta-Glucosidases - The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.04-22-2010
20110269183RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE - The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.11-03-2011
20110269185Compositions and Methods for Producing Fermentation Products and Residuals - The present invention provides compositions and methods designed to increase value output of a fermentation reaction that yields a first product, intended for commercialization, such as ethanol, and a fermentation residual used, for example, as animal feed. The methods involve using microorganisms in the fermentation process that have been modified so as to yield a residual having greater value that a residual produced in the process by a microorganism not so modified. In particular, the present invention contemplates using microorganisms in a fermentation process that have been modified to increase production of a nutrient, such as an essential amino acid, thereby reducing the need to supplement the nutrient in the animal's diet. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.11-03-2011
20100035300Producing a Target Protein Using Intramolecular Cleavage by TEV Protease - A cis-TEVP fusion protein including a TEVP protease, a TEVP cleavage site and a target protein provide a platform for expression of the target protein. A trans-TEVP fusion protein including a TEVP cleavage site and a target protein, the amino-terminal portion of the target protein adjacent to the C-terminal portion of the TEVP cleavage site, the amino acidic residue in position P2 of the TEVP cleavage site being a Valine also produces the target protein by the same process. A cis-TEVP fusion protein system comprising the first fusion protein and a suitable host cell; a trans-TEVP fusion protein system comprising the second fusion protein and a suitable host cell; associated methods to produce target proteins, and kits of parts are also disclosed herein.02-11-2010
20110217733Expanding the eukaryotic genetic code - This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.09-08-2011
20090081732ANTIBODIES TO HUMAN IL-1BETA - An IL-1β binding molecule, in particular an antibody to human IL-1β, especially a human antibody to human IL-1β is provided, wherein the CDRs of the heavy and light chains have amino acid sequences as defined, for use in the treatment of an IL-1 mediated disease or disorder, e.g. osteoarthritis, osteoporosis and other inflammatory arthritides.03-26-2009
20090325227C-TERMINUS MODIFICATION METHOD, C-TERMINUS IMMOBILIZATION METHOD AND ANALYSIS METHOD FOR PROTEIN OR PEPTIDE - The present invention provides a method for inexpensively, easily and efficiently modifying the C-terminus of a protein or peptide; a method for easily and reliably isolating a C-terminal peptide fragment of a protein or peptide; and a method for rapidly, accurately and reliably determining an amino acid sequence of a protein or peptide by using a mass spectroscope. A method comprising the step of adding a formylation reagent and a catalyst to a protein or peptide to convert a carboxyl group into an aldehyde group. A method comprising the step of reacting a nucleophilic reagent with the aldehyde group to modify the C-terminus of the protein or peptide. A method comprising the step of reacting a support having a nucleophilic group to immobilize the protein or peptide. A method comprising the step of fragmenting the immobilized protein or peptide, washing the support, and isolating the C-terminal peptide fragment from the support. A method comprising the step of subjecting the isolated C-terminal peptide fragment to mass spectrometry and determining an amino acid sequence.12-31-2009
20090098601Selection of Host Cells Expressing Protein at High Levels - The invention provides a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein said DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG startcodon or a TTG startcodon, and wherein the open reading frame sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native open reading frame sequence that encodes the selectable marker protein.04-16-2009
20090098600THERMOTOLERANT RIBONUCLEASE H - Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.04-16-2009
20090098602Bacillus mHKcel Cellulase - The present invention provides a novel cellulase nucleic acid sequence, designated mHKcel, and the corresponding mHKcel amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding mHKcel, recombinant mHKcel proteins and methods for producing the same.04-16-2009
20090181429Systems for the Expression of Orthogonal Translation Components Eubacterial Host Cells - The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.07-16-2009
20080311621POLYPEPTIDES AND NUCLEIC ACIDS ENCODING SAME - DNAs are provided, whose genes are induced at least by Wnt-1. Also provided are nucleic acid molecules encoding those polypeptides, as well as vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides, and methods for producing the polypeptides.12-18-2008
20080311618Expression System, Components Thereof and Methods of Use - Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from 12-18-2008
20090162898HIGH COPY NUMBER SELF-REPLICATING PLASMIDS IN PSEUDOMONAS - Provided herein are improved copy number plasmids, particularly those plasmids capable of replication in a bacterial cell. The improved copy number plasmid contain a deletion, insertion, or substitution in the replication control region, particularly a 06-25-2009
20100112638RECOMBINANT HOST CELL FOR THE PRODUCTION OF A COMPOUND OF INTEREST - We describe a recombinant host cell for the production of a compound of interest as well as isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding a compound of interest. We also describe methods for expressing a gene of interest and/or producing compounds of interest using a promoter according to the invention.05-06-2010
20090148902Polypeptides having endoglucanase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.06-11-2009
20090148903Polypeptides having beta-glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.06-11-2009
20090148899METHOD FOR PRODUCING OPTICALLY-ACTIVE AMINE COMPOUND, RECOMBINANT VECTOR, AND TRANSFORMANT CONTAINING THE VECTOR - The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an α-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.06-11-2009
20110059485Plasmids from Thermophilic Organisms, Vectors Derived Therefrom, and Uses Thereof - The present invention is directed to a replicative, thermostable plasmid. In particular, the present invention is directed to a replicative, thermostable plasmid comprising a sequence derived from the pB6A plasmid and at least one functional unit comprising a sequence that is not found in plasmid pB6A.03-10-2011
20090148901Polypeptides having xylanase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.06-11-2009
20090148894METHODS FOR OPTIMIZING THE SECRETION OF PROTEIN IN PROKARYOTES - Methods are provided for producing recombinant proteins by utilizing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the periplasm or extracellular medium. Expression vectors which encode a fusion protein comprising a carrier protein and the protein are also provided, as are host cells transformed with the expression vectors.06-11-2009
20090148900HEMCM42 NUCLEIC ACIDS - The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.06-11-2009
20090148898Human Ron-Related Gene Variant Associated With Cancers - The invention relates to the nucleic acid and polypeptide sequences of three novel human Ron-related gene variants (Ron-V1, Ron-V2, and Ron-V3). The invention also provides a process for producing the polypeptides of the variants, as well as uses for the nucleic acid, polypeptide and antibodies to same in diagnosing human breast carcinoma, breast adenocarcinoma, cervix epidermoid carcinoma, cervix epitheloid carcinoma, colon adenocarcinoma, urinary bladder carcinoma, prostate carcinoma, esophagus epidermoid carcinoma and esophagus carcinoma.06-11-2009
20090148897Human Ron-Related Gene Variant Associated With Cancers - The invention relates to the nucleic acid and polypeptide sequences of three novel human Ron-related gene variants (Ron-V1, Ron-V2, and Ron-V3). The invention also provides a process for producing the polypeptides of the variants, as well as uses for the nucleic acid, polypeptide and antibodies to same in diagnosing human breast carcinoma, breast adenocarcinoma, cervix epidermoid carcinoma, cervix epitheloid carcinoma, colon adenocarcinoma, urinary bladder carcinoma, prostate carcinoma, esophagus epidermoid carcinoma and esophagus carcinoma.06-11-2009
20090148893Novel Subtilases - The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.06-11-2009
20090148892NOVEL METHODS - This present invention relates to the use of the B1 domain of Protein G as an epitope tag for over-expression of proteins in mammalian cells.06-11-2009
20090148889Subtilases - The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.06-11-2009
20100267086Optimized Nucleic Acid Sequences For The Expression of VB4-845 - An optimized nucleic acid sequence encoding the immunoconjugate VB4-845 is disclosed. Modifications to the original VB4-845 nucleic acid sequence include optimization of the sequences encoding the V10-21-2010
20100267084PROTEIN GLYCOSYLATION MODIFICATION IN METHYLOTROPHIC YEAST - The present invention provides genetically engineered strains of 10-21-2010
20100267083Modified Cytochrome P450 Monooxygenases - The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.10-21-2010
20080293100Method for the Fermentative Production of L-Amino Acids With the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source - The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (>0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product. In said method, bacteria are used in which other genes of the biosynthesis pathway of the desired L-amino acid are additionally reinforced or in which the metabolism pathways reducing the formation of the desired L-amino acid are eliminated at least in part.11-27-2008
20080280326Novel Gonadotropin-Releasing Hormone, Precursor Peptides Thereof and Genes Encoding the Same - A novel peptide found as gonadotropin-releasing hormone (GnRH) in octopuses, a member of mollusks. The structure and the activities of the peptide have been unraveled. The peptide finds use as a reagent used in the studies of correlations between the structure and the activities of GnRH and the information processing mechanisms of the nervous systems in higher animals. It can also serve as a base compound in the development of pesticides and drugs. The peptide has the GnRH activity and has the following amino acid sequence (I):11-13-2008
20100279344Integrated cytokine production system - A multi-stage method for producing a cytokine involves expressing the cytokine in an expression system, preferably a 11-04-2010
20080261273PROCESS FOR PRODUCING HMG-CoA REDUCTASE INHIBITORS - The present invention relates to processes for producing compounds by using a protein derived from a microorganism belonging to the genus 10-23-2008
20120034654METHOD FOR TRANSFORMING SCHIZOSACCHAROMYCES POMBE, TRANSFORMANT OF SCHIZOSACCHAROMYCES POMBE AND METHOD FOR PRODUCING HETEROLOGOUS PROTEIN - To provide a method for transforming 02-09-2012
20090253167Methanol-responsive promoter, fusion gene comprising promoter and foreign gene connected so that foreign gene can be expressed, vector, transformant, and method of producing protein - A methanol-responsive promoter includes the following regions (1) and (2) in this order in the direction from its 5′- to 3′-terminal (1) a methanol-responsive region that is selected from the group consisting of the following (a), (b) and (c) (a) a polynucleotide consisting of the nucleotide sequence of SEQ ID No 1, (b) a polynucleotide consisting of the nucleotide sequence of SEQ ID No 1 wherein one or more nucleotides are deleted, substituted or added and the polynucleotide has methanol-responsive activity, and (c) a polynucleotide which hybridizes, under a stringent condition, to a polynucleotide consisting of nucleotide sequence complementary to all or part of the nucleotide sequence of SEQ ID No 1, and which has methanol-responsive activity, and (2) a core promoter region which is the minimum region necessary for transcription initiation and which has a binding site for RNA polymerase.10-08-2009
20090221035SYSTEMS AND METHODS FOR PROTEIN PRODUCTION - The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.09-03-2009
20090098604Synthetic gene encoding human epidermal growth factor 2/NEU antigen and uses thereof - Synthetic polynucleotides encoding human HER2/neu or a truncated form thereof, are provided, the synthetic polynucleotides being codon-optimized for expression in a human cellular environment. The gene encoding hHER2 is commonly associated with the development of human carcinomas. The present invention provides compositions and methods to elicit or enhance immunity to the protein product expressed by the hHER2 tumor-associated antigen, wherein aberrant hHER2 expression is associated with a carcinoma or its development. This invention specifically provides adenoviral vector and plasmid constructs carrying codon-optimized human HER2 and codon-optimized truncated HER2, and discloses their use in vaccines and pharmaceutical compositions for preventing and treating cancer.04-16-2009
20090087881Oncokinase fusion polypeptides associated with hyperproliferative and related disorders, nucleic acids encoding the same and methods for detecting and identifying the same - Oncokinase fusion polypeptides associated with hyperproliferative disorders and the polynucleotides encoding for such fusion polypeptides are provided. The fusion polypeptides have a C-terminal tyrosine kinase domain fused to an N-terminal domain that is not normally fused to the C-terminal tyrosine kinase domain and they possess constitutively activated tyrosine kinase activity. Also provided are methods for detecting and identifying the fusion polypeptides and polynucleotides and methods of diagnosing disease conditions associated with the fusion polypeptides and polynucleotides. In addition, screening assays for identifying agents useful for treating disease conditions associated with such fusion polypeptides and polynucleotides are provided. Furthermore, methods of treating disease conditions associated with the presence of the fusion polypeptides are provided.04-02-2009
20120142053METHOD FOR METHANOL INDEPENDENT INDUCTION FROM METHANOL INDUCIBLE PROMOTERS IN PICHIA - A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.06-07-2012
20100086967CELL GROWTH - The invention concerns the field of cell culture technology. It concerns a method of improving cell growth, especially the growth of biopharmaceutical producer host cells. The invention further concerns a method of producing proteins using the cells generated by the described method.04-08-2010
20100086968LACTIC ACID BACTERIA STRAIN AND ITS USE FOR THE PROTECTION OF FOOD PRODUCTS - The present invention refers to a strain of 04-08-2010
20100081171METHOD FOR LYOPHILIZING COMPETENT CELLS - This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.04-01-2010
20090258391RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING SAME - Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.10-15-2009
20090263861HOST-VECTOR SYSTEM ANTIBIOTIC-FREE ColE1 PLASMID PROPAGATION - A host-vector system that uses the RNA-based copy number control mechanism of ColE1-type plasmids for regulating the expression of a marker gene allows for antibiotic-free selection of plasmids and is useful for production of plasmid DNA and recombinant proteins.10-22-2009
20090263860Novel 13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 and 32252 molecules and uses therefor - The invention provides isolated nucleic acids molecules, designated 13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 and 32252 nucleic acid molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 and 32252 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 or 32252 gene has been introduced or disrupted. The invention still further provides isolated 13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 or 32252 proteins, fusion proteins, antigenic peptides and anti-13237, 18480, 2245, 16228, 7677, 26320, 46619, 33166, 16836, 46867, 21617, 55562, 39228, 62088, 46745, 23155, 21657, 42755, 32229, 22325, 46863 or 32252 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.10-22-2009
20090186380METHOD OF SECRETORY EXPRESSION OF LYSOSTAPHIN IN ESCHERICHIA COLI AT HIGH LEVEL - A method of secretory expression of lysostaphin in 07-23-2009
20090181425Library of Translational Fusion Partners for Producing Recombinant Proteins and Translational Fusion Partners Screened Therefrom - The invention relates to techniques for the rapid screening of suitable translational fusion partners (TFPs) capable of inducing secretory production of recombinant proteins, especially proteins that are difficult to produce using conventional recombinant production methods.07-16-2009
20090170161GDEP Enhancer Element and Use Thereof to Confer Retinal Specific Gene Expression - The present invention is directed to isolated nucleic acids containing functional polynucleotide sequences representing an enhancer element for the Gene Differentially Expressed in Prostate (GDEP). Such molecules are useful in conferring retinal specific transcriptional responsiveness on associated promoters and methods for directing retinal specific gene expression are accordingly disclosed.07-02-2009
20100099143Alanine 2,3-aminomutases and related polynucleotides - The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the AAM polypeptides, and to a method for producing the AAM polypeptides of the present invention.04-22-2010
20090142804Process For Preparing Serine-Rich Protein Employing Cysteine Synthase (CYSK) Gene - The present invention relates to a process for preparing a serine-rich foreign protein comprising culturing a bacterium containing the cysteine synthase (cysK) gene and a gene encoding the serine-rich foreign protein. The present invention comprises the steps of culturing a bacterium transformed with an expression vector containing a gene encoding a serine-rich foreign protein and an expression vector containing the cysK gene, or a bacterium transformed with an expression vector containing the cysK gene and a gene encoding a serine-rich foreign protein and isolating the foreign protein therefrom. The present invention is expected to be widely used to increase the production yield of a serine-rich foreign protein.06-04-2009
20090142800Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.06-04-2009
20100093033INDUCIBLE MUTAGENESIS OF TARGET GENES - The present invention relates generally to mutagenesis of target genes that takes advantage of the natural mutagenic capabilities of B cells, and enhances those capabilities by bringing the process of diversification under control. The invention provides a method for rapidly and inducibly generating point mutations and other types of diversification in expressed genes, such as antibody genes. This method can be coupled with selection to identify B cell clones that produce, for example, antibodies of high affinity or specificity. The diversification process can be modulated, accelerated, halted, switched between methods of mutagenesis and the like. The modulation of diversification in accordance with the invention is both inducible and reversible. The invention provides a means of rapid and feasible development of a repertoire of variant immunoglobulins and other polypeptides.04-15-2010
20100081170METHODS FOR PRODUCING SOLUBLE MEMBRANE-SPANNING PROTEINS - Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.04-01-2010
20090253168Novel Transporter Protein - A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From 10-08-2009
20120034653Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering - Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga 02-09-2012
20090203075GENES CONFERRING HERBICIDE RESISTANCE - Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 3 or the nucleotide sequence set forth in SEQ ID NO: 1 or 2.08-13-2009
20090186381CELLULASE VARIANTS WITH REDUCED INHIBITION BY GLUCOSE - A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.07-23-2009
20090170154Mutant bacterium belonging to the genus bacillus - The present invention provides a mutant 07-02-2009
20120295307PICHIA PASTORIS DEFICIENT IN ENDOGENOUS SECRETED PROTEASE - The present invention relates to micro-organisms and to methods of producing proteins. More specifically, the inventions relates to a host cell useful for the expression of heterozygous proteins in which the host cell, 11-22-2012
20090162899MANNITOL INDUCED PROMOTER SYSTEMS IN BACTERIAL HOST CELLS - The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.06-25-2009
20090155845Molecules designated LDCAM - The invention is directed to LDCAM as a purified and isolated protein, the DNA encoding the LDCAM, host cells transfected with cDNAs encoding LDCAM, processes for preparing LDCAM polypeptides and compositions and methods for treating utilizing LDCAM polypeptides.06-18-2009
20100099145RECOMBINANT HUMAN EPO-FC FUSION PROTEINS WITH PROLONGED HALF-LIFE AND ENHANCED ERYTHROPOIETIC ACTIVITY IN VIVO - A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.04-22-2010
20090142802POLYPEPTIDES AND NUCLEIC ACIDS ENCODING SAME - The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from 06-04-2009
20090291471Novel Beta-Galactoside Alpha 2,6-Sialyltransferase, Gene Coding For The Transferase And Process For Producing The Same - The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.11-26-2009
20090291470Chondroitin synthase, method for producing the same and method for producing saccharide chain-extended chondroitin - A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.11-26-2009
20080206809PEPTIDE TAGS FOR THE EXPRESSION AND PURIFICATION OF BIOACTIVE PEPTIDES - Peptide tags, referred to here as inclusion body tags, are disclosed and are useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.08-28-2008
20080206810Truncated St6galnaci Polypeptides and Nucleic Acids - The present invention features compositions and methods related to truncated mutants of ST6GalNAcI. In particular, the invention features truncated human, mouse, and chicken ST6GalNAcI polypeptides. The invention also features nucleic acids encoding such truncated polypeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.08-28-2008
20090280529HIGH THROUGHPUT TRANSFECTION OF FILAMENTOUS FUNGI - The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.11-12-2009
20090280533SERUM-FREE MAMMALIAN CELL CULTURE MEDIUM, AND USES THEREOF - The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.11-12-2009
20090286280METHOD FOR ACHIEVING IMPROVED POLYPEPTIDE EXPRESSION - The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimisation of single codon fitness and/or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence for a predetermined amino acid sequence, the coding sequence being optimised with respect to single codon fitness and/or codon pair fitness for a given host in the methods of the invention, to host cells comprising such nucleic acids and to methods for producing polypeptides and other fermentation products in which these host cells are used.11-19-2009
20100279350C-type lectin polypeptide, polynucleotide and methods of making and use thereof - Provided herein are polypeptide and polynucleotide sequences for a molecule having homology to the C-type lectin family of polypeptides. Also provided are methods of making and using the polypeptide and polynucleotides.11-04-2010
20110171686REVERSE CUMATE REPRESSOR MUTANT - Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from 07-14-2011
20090253177Maize Cellulose Synthases and Uses Thereof - The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.10-08-2009
20100279346Compositions and Methods for Improved Protein Production - The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p,8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p,8k, 7E, 9G, 8Q and 203, which are presented in FIG. 11-04-2010
20100279347POLYMYXIN SYNTHETASE AND GENE CLUSTER THEREOF - The present invention relates to a polymyxin synthetase isolated from Gram-positive 11-04-2010
20110201052NUCLEIC ACIDS AND CORRESPONDING PROTEINS ENTITLED 202P5A5 USEFUL IN TREATMENT AND DETECTION OF CANCER - A novel gene 202P5A5 and its encoded protein, and variants thereof, are described wherein 202P5A5 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 202P5A5 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 202P5A5 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 202P5A5 can be used in active or passive immunization.08-18-2011
20080293104 Proteases and Methods for Producing Them - A secreted mature polypeptide derived from an S2A or S1E protease which after maturation has protease activity, which polypeptide when expressed and before maturation comprises a heterologous pro-region.11-27-2008
20120295308CARBOHYDATE BINDING MODULES WITH REDUCED BINDING TO LIGNIN - Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.11-22-2012
20080213834GSK3 LIGANDS AND POLYNUCLEOTIDES ENCODING GSK3 LIGANDS - The invention relates to kinase ligands and polyligands. In particular, the invention relates to ligands and polyligands that modulate GSK3 activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands and polyligands to a cellular localization signal, epitope tag and/or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.09-04-2008
20100196955Scaleable Manufacturing Process for Cysteine Endoprotease B, Isoform 2 - Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product.08-05-2010
20090170158Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.07-02-2009
20100136621SOLUBILITY TAGS FOR THE EXPRESSION AND PURIFICATION OF BIOACTIVE PEPTIDES - Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.06-03-2010
20110201051CHEMOSENSORY GENE FAMILY ENCODING GUSTATORY AND OLFACTORY RECEPTORS AND USES THEREOF - This invention provides an isolated nucleic acid encoding an insect gustatory or odorant receptor. This invention provides a nucleic acid of at least 12 nucleotides capable of specifically hybridizing with a nucleic acid encoding an insect gustatory or odorant receptor. This invention also provides a purified, insect gustatory or odorant receptor. This invention provides an antibody capable of specifically binding to an insect gustatory or odorant receptor. This invention provides a method of identifying a compound capable of specifically binding to, activating, or inhibiting the activity of an insect gustatory or odorant receptor. This invention also provides methods of controlling insect populations.08-18-2011
20080274498Methods for producing modified glycoproteins - Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man11-06-2008
20080274500Promoter variants for expressing genes in a fungal cell - The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.11-06-2008
20080274499Polypeptides having alpha-glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.11-06-2008
20090170155Mutant Endoglycoceramidases With Enhanced Synthetic Activity - The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.07-02-2009
20100167346METHOD OF PRODUCING HETEROGENEOUS PROTEIN - The present invention provides a method capable of producing a natural or recombinant protein in high yield.07-01-2010
20080280325Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.11-13-2008
20080293103STRESS PROTEIN COMPOSITIONS AND METHODS FOR PREVENTION AND TREATMENT OF CANCER AND INFECTIOUS DISEASE - Pharmaceutical compositions comprising a stress protein complex and related molecules encoding or cells presenting such a complex are provided. The stress protein complex comprises an hsp110 or grp170 polypeptide complexed with an immunogenic polypeptide. The immunogenic polypeptide of the stress protein complex can be associated with a cancer or an infectious disease. Preferred immunogenic polypeptides include gp100, her2/neu ECD-PD, ICD and 11-27-2008
20080293102COMPOSITIONS AND METHODS FOR PRODUCING APOLIPOPROTEIN - The disclosure relates to recombinant nucleic acids, expression vectors comprising the recombinant nucleic acids, and host cells comprising the expression vectors for expressing a protein of interest.11-27-2008
20080293099Lignan Glycosidase and Utilization of the Same - The present invention provides an enzyme having the lignan glycosidation activity by identifying the enzyme that is involved in the production of lignan glycosides, identifying the amino acid sequence of the enzyme polypeptide and the base sequence for a polynucleotide encoding the polypeptide, and based on the information of these sequences, preparing the transformants capable of producing the lignan glycosides.11-27-2008
20080305521Method for Overproducing Specific Recombinant Protein with P. Cinnabarinus Monokaryotic Strains - The use of monokaryotic strains of filamentous fungi of the 12-11-2008
20080305523Fungal Promoter for Expressing a Gene in a Fungal Cell - The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.12-11-2008
20080305522Novel Taste-Modifying Polypeptide Nas, Dna Thereof and Use Thereof - It is an object of the invention to find a substance with a better taste-modifying function and determine the structure of the taste-modifying substance, as well as to elucidate the structure thereof at a gene level and determine the primary structure of the substance and obtain the gene encoding the substance. Additionally, it is an object of the invention to provide a novel taste-modifying composition characteristically containing the taste-modifying substance.12-11-2008
20080213831HUMAN CYTOKINE RECEPTOR - Cytokines and their receptors have proven usefulness in both basic research and as therapeutics. The present invention provides a new human cytokine receptor designated as “Zcytor18.”09-04-2008
20120295306Modified CIPA Gene From Clostridium Thermocellum for Enhanced Genetic Stability - Bacteria consume a variety of biomass-derived substrates and produce ethanol. The scaffoldin gene cipA from 11-22-2012
20080213830Degradable Clostridial Toxins - The specification discloses modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; polynucleotide molecules encoding modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; and method of producing modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain.09-04-2008
20080280324Glycoengineering in Mushrooms - The invention relates to the field of genetic engineering, more specifically to the field of production of glycoproteins, more specifically to the engineering of the glycosylation pattern of glycoproteins. Provided is a method for producing a glycoprotein in a recombinant host cell, comprising allowing expression of said glycoprotein in a basidiomycete host provided with N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase (GnT-I; EC 2.4.1.101) activity. Also provided is a basidiomycente host cell expressing GNT-I, preferably human GnT-I and use thereof for the manufacture of (mammalian) proteins carrying complex N-glycans, such as (monoclonal) antibodies.11-13-2008
20080305524Molecular Glue - The present invention relates to an adhesive material being composed and/or consisting of at least one protein obtained or obtainable from flagella from archaea. Furthermore, the present invention relates to the use of at least one protein obtained from flagella from archaea for the method for the preparation of an adhesive material comprising the step of isolating and/or purifying at least one protein obtained from flagella from archaea.12-11-2008
20080318273MUTANT PROTEINASE WITH REDUCED SELF-CLEAVAGE ACTIVITY AND METHOD OF PURIFICATION - The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant has the same substrate cleavage activity as the wild-type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins.12-25-2008
20080274504SOLUBLE ErbB3 RECEPTOR ISOFORMS - The present invention discloses a method using human soluble ErbB3, for example p85-sErbB3, as a negative regulator of heregulin-stimulated ErbB2, ErbB3, and ErbB4 activation. The present invention also discloses p85-sErbB3 binding to heregulin with an affinity comparable to that of full-length ErbB3, and competitively inhibiting high affinity heregulin binding to ErbB2/ErbB3 heterodimers on the cell surface of breast carcinoma cells. The present invention also uses p85-sErbB3 to inhibit heregulin-induced phosphorylation of ErbB2, ErbB3, and ErbB4 in cells, as a negative regulator of heregulin-stimulated signal transduction, and as a block for cell growth. The present invention is also directed to nucleic acids and expression vectors encoding p85-sErbB3, host cells harboring such expression vectors, and methods of producing the protein. The present invention discloses a method of therapeutically treating human malignancies associated with heregulin-mediated cell growth such as breast and prostate cancer.11-06-2008
20080206811Process For Producing Polypeptide - A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.08-28-2008
20080213836Modified enteropeptidase protein - Disclosed are novel enteropeptidase polypeptides, polynucleotides encoding the polypeptides, nucleotide constructs, vectors, host cells comprising the polynucleotides, and methods for producing the polypeptides and polynucleotides. Such polypeptides are useful as protein engineering tool for enzymatic cleavage of fusion proteins. Also provided are kits comprising the polypeptides of the invention.09-04-2008
20080261272FUNGAL CELL WALL SYNTHESIS GENE - A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.10-23-2008
20090004693Production of Polypeptides by Improved Secretion - The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest.01-01-2009
20090004695Process for constructing strain having compactin hydroxylation ability - The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to express both cytochrome P-450 and the fdxshe or fdxshe-like protein. The invention further relates to the use of such microorganisms in processes designed for production of pravastatin for use in treatment of disease such as hypercholesterolemia and hyperlipidemia.01-01-2009
20080268502Multiple Promoters and the Use Thereof For Gene Expression - The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.10-30-2008
20090093018METHOD OF PRODUCING HETERODIMER DERIVATIVE OF PROTEIN PHOSPHATASE TYPE 2A ENZYME - The purpose of the invention is to provide an activated protein phosphatase 2A (PP2A) in large quantities with high purity by a genetic engineering and to provide a method for producing a heterodimer derivative of PP2A which comprises infecting insect cultured cells with a baculovirus in which a cDNA encoding the catalytic subunit of PP2A carrying a first tag is integrated together with another baculovirus in which a cDNA encoding the A subunit of PP2A carrying a second tag is integrated, incubating the infected cells, disrupting the incubated cells to obtain a disrupted cell suspension, and then purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the first tag and another solid phase carrying a substance capable of binding to the second tag, characterized in that the insect cells infected with the baculovirus are incubated at a temperature of from 18 to 22° C.04-09-2009
20090181426Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants - The invention relates to cell culture methods, kits and cell lines for producing recombinant products, e.g. therapeutic proteins and antibodies, in the presence of reduced levels of one or more contaminants and further to methods of purifying those products.07-16-2009
20120142052METHOD AND DEVICE FOR MULTIPLE FEED CULTIVATION - The current invention is directed to support units for mounting a cell cultivation liquid storage unit which can be connected to a balance, a cell cultivation apparatus using such a support unit, and the use of such a support unit in a method for the cultivation of cells.06-07-2012
20120196324Method for Producing an Antifungal Peptide in a Filamentous Fungal Host Cell - The present invention provides a method for producing an antifungal peptide in a filamentous fungal host cell by expressing the antifungal peptide in a host cell which is deficient or partially deficient in the expression of an endogenous glucosylceramide synthase (gcs) gene.08-02-2012
20110269184METHOD FOR CONTROLLING PLASMID COPY NUMBER IN E.COLI - Host vector system and methods for plasmid DNA and recombinant protein production. The system allows copy number control of a ColE1 plasmid in 11-03-2011
20090053761Polypeptide Mutagenesis Method - There is provided a method for altering the amino acid sequence of a target polypeptide by altering a target DNA sequence which encodes that polypeptide, the method comprising the step of introducing a transposon into the target DNA sequence, in which the transposon comprises a first restriction enzyme recognition sequence towards each of its termini, the recognition sequence not being present in the remainder of the transposon, or in the target DNA sequence, or in a construct comprising the target DNA sequence, the first restriction enzyme recognition sequence being recognised by a first restriction enzyme which is an outside cutter and being positioned such that the first restriction enzyme has a DNA cleavage site positioned beyond the end of the terminus of the transposon.02-26-2009
20090081724Mutations in Ion Channels - A method of identifying a subject predisposed to a disorder associated with ion channel dysfunction, comprising ascertaining whether at least one of the genes encoding ion channel subunits in said subject has undergone a mutation event as set forth in one of SEQ ID Numbers: 1-72.03-26-2009
20090136999COMPOSITIONS AND METHODS FOR IMMUNOTHERAPY OF CANCER AND INFECTIOUS DISEASES - The present invention provides compositions and methods for the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases, for stimulating an immune response in a subject, and for use as an alternative to interleukin-12 (IL-12) treatment. In particular, the present invention provides Apicomplexa-related proteins (ARPs) that have immune stimulatory activity and thus have uses in the treatment and prevention of cancer and infectious diseases and in immune modulation. Compositions comprising an ARP are provided. Methods of use of an ARP for the prevention and/or treatment of cancer and/or infectious diseases, for use as an alternative to interleukin-12 (IL-12) treatment, and for eliciting an immune response in a subject, are also provided.05-28-2009
20090162897Promoter Sequences - The present invention relates to methods for producing polypeptides or nucleic acids, in particular antisense RNA and hairpin RNA in filamentous fungi. The present invention also relates to isolated 06-25-2009
20090162896Production of Recombinant Collagen Like Proteins - The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.06-25-2009
20090186378Polynucleotides encoding the fkbB gene of the FK-520 polyketide synthase gene cluster - Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.07-23-2009
20090291472INFLUENZA NUCLEIC ACIDS, POLYPEPTIDES, AND USES THEREOF - Codon-optimized nucleic acids encoding influenza polypeptides and uses of the nucleic acids and polypeptides for inducing immune responses are provided herein.11-26-2009
20090136997PRO1550 POLYPEPTIDES AND NUCLEIC ACIDS ENCODING THE SAME - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.05-28-2009
20090136995Mutated Prokaryotic Cells with High Secretion-Levels - A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides.05-28-2009
20090136994Polypeptide having amidase activity and gene thereof - It is an object of the present invention to provide a novel amidase that is useful for production of an optically active amino acid, and in particular, a D-amino acid, and a production method thereof.05-28-2009
20090142801BTL-II NUCLEIC ACIDS - The invention provides isolated BTL-II proteins, nucleic acids, antibodies, antagonists, and agonists and methods of making and using the same. Diagnostic, screening, and therapeutic methods using the compositions of the invention are provided. For example, the compositions of the invention can be used for diagnosis and treatment of inflammatory bowel diseases and for enhancing a mucosal immune response to an antigen.06-04-2009
20080318272Gene Encoding Methylated Catechin Synthase - A gene encoding a methylated catechin synthase that can effectively synthesize methylated catechins having high antiallergic activity.12-25-2008
20090246829SEQUENCE ANALYSIS OF THE TETRAVALENT ROTAVIRUS VACCINE - Isolated nucleic acid molecules comprising a gene segment from a rhesus rotavirus (RRV) or from one of three rhesus:human reassortant viruses are disclosed, including isolated nucleic acid molecules having a sequence selected from the group consisting of: SEQ ID NO: 1-14, inclusive, and isolated nucleic acid molecules encoding a protein having a sequence selected from the group consisting of SEQ ID NO: 15-28, inclusive, as well as variants of the isolated nucleic acid molecules.10-01-2009
20090325228FUNGAL CELL WALL SYNTHESIS GENE - A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.12-31-2009
20090325226Methods and Compositions for Producing Active Vitamin K-Dependent Proteins - The present invention provides a method of identifying a human subject having increased or decreased sensitivity to warfarin, comprising detecting in the subject the presence of a single nucleotide polymorphism in the VKOR gene, wherein the single nucleotide polymorphism is correlated with increased or decreased sensitivity to warfarin, thereby identifying the subject having increased or decreased sensitivity to warfarin.12-31-2009
20090023184Novel Tachykinin Peptides, Precursor Peptides Thereof And Genes Encoding The Same - Tachykinin peptide isolated and purified from the posterior salivary gland 01-22-2009
20090023183Method For Preparing Recombinant Peptide From Spider Venom and Analgesic Composition Containing The Peptide - The present invention relates to a method for producing a recombinant, spider toxin peptide and analgesic compositions containing said peptide. More specifically, the present invention relates to a method in which the gene for GsMTx4 is subcloned into a vector, so that it is linked to a secretion signal sequence of the alpha factor and under the control of methanol-inducible alcohol oxidase (AOX) promoter to construct a recombinant yeast expression plasmid. Yeast cells are transformed with this plasmid to produce the GsMTx4 peptide and analgesic compositions containing said peptide. The recombinant yeast expression system of the present invention affords a more stable method for producing GsMTx4 than its natural route. Thus the GsMTx4 peptide and its derivatives produced by the method of this invention can be used in the cure of related diseases such as heart failure as the peptide specifically inhibits mechanosensitive ion channels.01-22-2009
20090047707Bacterial recombinant phytase - A novel phytase enzyme obtainable from B. Subtilis strain ARRMK-33 is disclosed. Also a novel method to produce recombinant phytase protein in prokaryotic cells is disclosed.02-19-2009
20090258390Prokaryotic collagen-like proteins and uses thereof - The present invention provides recombinant triple helical proteins or collagen-like proteins comprising a prokaryotic protein or one or more domains of a prokaryotic protein comprising a collagen-like peptide sequence of repeated Gly-Xaa-Yaa triplets and, optionally, one or more domains from a mammalian collagen. Also provided are expression vectors and host cells containing the expression vectors to produce these recombinant proteins and methods of producing the same. Additionally, antibodies are provided that are directed against a recombinant collagen-like protein that, preferably, binds an integrin. Furthermore, a method of screening for potential therapeutic compounds that inhibit the integrin-binding or integrin-interacting activities of recombinant collagen-like proteins.10-15-2009
20130217068NOVEL FUCOSYLTRANSFERASES AND THEIR APPLICATIONS - The present invention relates to nucleic acid and amino acid sequences from 08-22-2013
20090098605MUTATED TRUNCATED mt-pfkA GENE FOR THE SYNTHESIS OF ACTIVE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-1-KINASE - The invention deals with mutated truncated mX-pfkA gene encoding shorter fragment of 6-phosphofructo-1-kinase (PFK1), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49-52 kDa fragment encoded by mt-pfltk gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.04-16-2009
20090098606Methods for producing biological substances in pigment-deficient mutants of bacillus cells - The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent 04-16-2009
20090081730ACTIVATABLE RECOMBINANT NEUROTOXINS - Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids.03-26-2009
20090081726Recombinant Microorganism - A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.03-26-2009
20090081729Compositions and Methods Comprising a Ligand of ChemerinR - The present invention relates to a G-protein coupled receptor and a novel ligand therefor. The invention provides screening assays for the identification of candidate compounds which modulate the activity of the G-protein coupled receptor, as well as assays useful for the diagnosis and treatment of a disease or disorder related to the dysregulation of G-protein coupled receptor signaling.03-26-2009
20090081727CD33-Like Protein - The present invention concerns a novel CD33-like protein. In particular, isolated nucleic acid molecules are provided encoding the CD33-like protein. Recombinant CD33-like polypeptides are also provided as are recombinant vectors and host cells. The invention further provides methods useful during tumor or inflammatory disease diagnosis or prognosis and therapeutic treatments targeting cells expressing CD33-like polypeptides.03-26-2009
20090081725Compositions of pamps and Listeria monocytogenes and methods of use - A composition comprising a pathogen associated molecular pattern (PAMP) protein that activates toll-like receptor 2 (TLR2) or toll-like receptor 5 (TLR5) signaling and at least two distinct antigens of 03-26-2009
20090087879Method for the production of a lysate used for cell-free protein biosynthesis - The invention relates to a method for producing a lysate used for cell-free protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-free protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also disclosed are said lysate and the use thereof.04-02-2009
20090098603POLYPEPTIDES SHARING SEQUENCE IDENTITY WITH A FIBROBLAST GROWTH FACTOR POLYPEPTIDE AND NUCLEIC ACIDS ENCODING THE SAME - The present invention is directed to novel polypeptides having homology to the PRO533 protein and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention, and methods for producing the polypeptides of the present invention. The invention concerns compositions and methods for the diagnosis and treatment of neoplastic cell growth and proliferation in mammals, including humans. The invention is based on the identification of genes that are amplified in the genome of tumor cells. Such gene amplification is expected to be associated with the overexpression of the gene product and contribute to tumorigenesis and/or autocrine signaling. Accordingly, the proteins encoded by the amplified genes are believed to be useful targets for the diagnosis and/or treatment (including prevention) of certain cancers, and may act of predictors of the prognosis of tumor treatment. Furthermore, the compounds, compositions including antagonists and methods of the present invention are further expected to have therapeutic effect upon conditions characterized by FgF-19 modulation.04-16-2009
20090068702METHOD FOR PRODUCING A MUCIN-TYPE GLYCOPROTEIN - The present invention relates to a means for generating a mucin-type glycopeptide or glycoprotein on a large scale in yeast. Specifically, the invention relates to a method which comprises introducing into a yeast at least one selected from the group consisting of a gene encoding UDP-GalNAc synthetase, a gene encoding UDP-GalNAc transporter, and a gene encoding polypeptide:O-GalNAc transferase, and, if desired, a gene encoding a mucin-type glycopeptide; and producing a mucin-type glycoprotein having O-GalNAc by use of the yeast.03-12-2009
20090053766METHODS AND COMPOSITIONS FOR PRODUCING RECOMBINANT PROTEINS USING A GENE FOR TRNA - The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.02-26-2009
20090087882Protein secretion in eukaryotic cells - The invention relates to the use of a glucosidase II mutation to increase protein secretion in yeast cells. The invention relates further to the use of yeast cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.04-02-2009
20090191590Signal Peptide for Producing a Polypeptide - The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.07-30-2009
20110223635Method and Composition for Controlling Gene Expression - A composition for expressing a protein in cells is provided. In certain embodiments, a circular expression vector provided herein comprises: a promoter, a coding sequence encoding a protein of interest, in which the coding sequence is in a reversed 3′-5′ orientation, a transcription termination sequence, and at least a first recombination site and a second recombination site flanking the coding sequence. A method for using the disclosed composition and a kit comprising the composition are also provided herein.09-15-2011
20090053764Salutaridinol 7-0-Acetyltransferase and Derivatives Thereof - This invention provides a protein comprising consecutive amino acids, the amino acid sequence of which is illustrated in FIG. 02-26-2009
20090098599Adenovirus vectors, packaging cell lines, compositions, and methods for preparation and use - The present invention relates to methods for gene therapy, especially to adenovirus-based gene therapy, and related cell lines and compositions. In particular, novel nucleic acid constructs and packaging cell lines are disclosed, for use in facilitating the development of high-capacity and targeted vectors. The invention also discloses a variety of high-capacity adenovirus vectors and related compositions and kits including the disclosed cell lines and vectors. Finally, the invention discloses methods of preparing and using the disclosed vectors, cell lines and kits.04-16-2009
20080286833EXPRESSION VECTOR, A TRANSFORMANT CARRYING THE SAME AND A METHOD FOR PRODUCING HETEROLOGOUS PROTEIN - To provide a heterologous protein expression system which enables the fission yeast 11-20-2008
20090203073Gene Encoding G-Protein Coupled Receptor and Gene Product Thereof - The present invention, the objects of which are to find out a gene encoding a protein having functions equivalent to those of G-protein-coupled receptor (GPCR) and the protein, and to provide a method for identifying a compound that regulates functions and/or expression of the protein, and to provide a useful mean for preventing and/or treating diseases related to the gene and the protein, provides a DNA comprising base sequence described in SEQ ID NO: 1 or complementary strand thereof, complementary strand of the DNA, a protein encoded by the DNA, a vector containing the DNA, a transformant containing the vector, an antibody against the protein, a method for identifying a compound that regulates functions and/or expression of the protein using aforementioned members, an agent for improving and a method for improving depression state, a pharmaceutical composition, and a reagent kit.08-13-2009
20090117616Methods for reducing or eliminating alpha-mannosidase resistant glycans for the production of glycoproteins - The present invention provides methods to reduce or eliminate α-mannosidase resistant glycans on glycoproteins in yeast. The reduction or elimination of α-mannosidase resistant glycans on glycoproteins results from the disruption of the newly isolated 05-07-2009
20090117615Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest - The present invention relates to a method of expressing an objective protein at a high level and stably as well as for a long period even in the absence of a selection drug with a recombinant mammal cell. More particularly, the present invention relates to a method of producing an objective protein by providing a recombinant mammal cell having multiple copies of the exogenous objective protein gene expression unit integrated into a hypoxanthine-phosphoribosyl transferase enzyme (hprt) gene locus and culturing said cell.05-07-2009
20090117614BETA-MANNANASE FROM COFFEE BERRY BORER, HYPOTHENEMUS HAMPEI, AND USES THEREOF - The present invention relates to an isolated β-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO: 1, as well as isolated polynucleotides encoding the β-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing β-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, which involves providing plant material and contacting the plant material with the β-mannanase protein of the present invention under conditions effective to degrade mannans and polysaccharides in the plant material.05-07-2009
20090203074Bacillus Host Cell08-13-2009
20090221028Novel Recombinant Human Hepatitis C Virus-Like Particle and Method for Producing the Same - The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5′ untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3′ untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.09-03-2009
20090221027Use of a bacillus meti gene to improve methionine production in microorganisms - The present invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing fine chemicals using the metI gene from 09-03-2009
20090221030SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL - The invention provides methods and compositions for improved protein production. The method comprises the steps of: (a) introducing into a host cell a first nucleic acid sequence comprising a signal sequence operably linked to a desired protein sequence; (b) expressing the first nucleic acid sequence; (c) co-expressing a second nucleic acid sequence encoding a chaperone or foldase selected from the group consisting of bip1, ero1, pdi1, tig1, prp1, ppi1, ppi2, prp3, prp4, calnexin, and lhs1; and (d) collecting the desired protein secreted from the host cell. The first nucleic acid sequence optionally comprises an enzyme sequence between the signal sequence and the desired protein sequence.09-03-2009
20090221032ENTROPIC BRISTLE DOMAIN SEQUENCES AND THEIR USE IN RECOMBINANT PROTEIN PRODUCTION - Compositions and methods for recombinant protein production and, more particularly, fusion polypeptides, polynucleotides encoding fusion polypeptides, expression vectors, kits, and related methods for recombinant protein production, are provided.09-03-2009
20120142050NOVEL EXPRESSION VECTOR - The present inventors constructed a transformation system which uses as a host the 06-07-2012
200902090012-Deoxy-D-Ribose 5-Phosphate Aldolases (DERAS) And Uses Thereof - The invention relates to isolated mutants of enzymes from the group of 2-deoxy-D-ribose 5-phosphate aldolase wild-type enzymes having a productivity factor (as determined by a specific test) which is at least 10% higher than the productivity factor for the corresponding wild-type enzyme from which it is a mutant. The mutants have at least one amino acid substitution at one or more of the positions corresponding to K13, T19, Y49, N80, D84, A93, E127, A128, K146, K160, I166, A174, M185, K196, F200, and S239 in 08-20-2009
20090221031NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS - The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.09-03-2009
20090239260Fungal Transcriptional Activator Useful In Methods for Producing Polypeptides - The present disclosure relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The disclosure also relates to nucleic acid constructs, vectors and host cells including the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.09-24-2009
20080261271EXPRESSION OF HIV POLYPEPTIDES AND PRODUCTION OF VIRUS-LIKE PARTICLES - The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides are described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Virus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for the presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.10-23-2008
20080261270Soluble ErbB3 Receptor Isoforms - The present invention discloses a system and method using isoforms of the human ErbB3 receptor as negative regulators of heregulin-stimulated ErbB2, ErbB3, and ErbB4 activation. The present invention also discloses a system and method of one such isoform, p85-sErbB3 binding to heregulin with an affinity comparable to that of full-length ErbB3, and competitively inhibiting high affinity heregulin binding to ErbB2/3 heterodimers on the cell surface of breast carcinoma cells. The present invention also uses p85-sErbB3 to inhibit heregulin-induced phosphorylation of ErbB2, ErbB3, and ErbB4 in cells and uses p85-sErbB3 as a negative regulator of heregulin-stimulated signal transduction and as a block for cell growth. The present invention is also directed to nucleic acids and expression vectors encoding p85-sErbB3, host cells harboring such expression vectors, and methods of preparing the protein. The present invention discloses a system and method of therapeutic applications in human malignancies associated with heregulin-mediated cell growth such as breast and prostate cancer.10-23-2008
20090253170Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same - Novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in trichomes are provided.10-08-2009
20090221029PFU REPLICATION ACCESSORY FACTORS AND METHODS OF USE - This invention provides isolated polynucleotides that encode replication accessory factors. The invention also provides novel DNA replication accessory factors, which have been isolated and purified from the hyperthermophilic archaea 09-03-2009
20090221033Polypeptides Having Lipase Activity And Polynucleotides Encoding Same - The invention provides polypeptides obtained by introducing mutations in one or more regions identified in a parent lipase. The polypeptides of the present invention have surprisingly been found to have a low specific activity towards short chain fatty acids leading to a reduced odor generation and an increased BR over the lipases known in the art.09-03-2009
20090258389Construction of New Variants of Dextransucrase DSR-S by Genetic Engineering - The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the α-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of α-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.10-15-2009
20100003718METHOD AND APPARATUS FOR THE PRODUCTION OF SOLUBLE MHC ANTIGENS AND USES THEREOF - The field of the invention relates in general to at least one method and apparatus for the production of soluble MHC antigens and more particularly, but not by way of limitation, to at least one method and apparatus for the production of soluble Class I and II HLA molecules. The field of the invention also includes such produced soluble Class I and II HLA molecules and their use. According to the methodology of the present invention, the soluble Class I and II HLA molecules can be produced from either gDNA or cDNA starting material.01-07-2010
20090239259MAMMALIAN EXPRESSION VECTORS AND USES THEREOF - The present invention features nucleic acids for recombinant protein expression in mammalian cell culture. The episomal vectors of the invention promote high protein production in mammalian cells expressing the SV40 T Ag or Epstein-Barr virus nuclear antigen (e.g., COS7 or HEK293-6E cells). The methods and systems are useful, for example, in pharmaceutical drug development and cloning, especially for the production of antibodies.09-24-2009
20090253171Methods For Producing Secreted Polypeptides Having Biological Activity - The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.10-08-2009
20090253176Cell lines and methods for producing proteins - The methods of the present invention involve the manipulation and/or propagation of oviduct tumor cells derived from either wild-type or transgenic avians.10-08-2009
20090253172GROWTH FACTOR HOMOLOG ZVEGF4 - Polypeptide growth factors, methods of making them, polynucleotides encoding them, antibodies to them, and methods of using them are disclosed. The polypeptides comprise an amino acid segment that is at least 70% identical to residues 52-179 of SEQ ID NO:2 or residues 258-370 of SEQ ID NO:2. Multimers of the polypeptides are also disclosed. The polypeptides, multimeric proteins, and polynucleotides can be used in the study and regulation of cell and tissue development, as components of cell culture media, and as diagnostic agents.10-08-2009
20090253174Expression of Heterologous Sequences - The present invention provides compositions and methods for expression of heterologous sequences. The compositions and methods are particularly useful for expressing large quantity of heterologous proteins and nucleic acids of therapeutic, diagnostic and industrial applications.10-08-2009
20100143971POLYPEPTIDES HAVING CELLULOLYTIC ENHANCING ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.06-10-2010
20100015662Use of cellobiohydrolase from phanerochaete - A cellobiohydrolase that can contribute to a synergistic effect on cellulose degradation and the use of such cellobiohydrolase in cellulose degradation are provided. The synergistic effect is achieved by an enzyme preparation for cellulose degradation containing a cellobiohydrolase originating in 01-21-2010
20100015660Production of Recombiant Products Using Capillary Membranes - The invention provides for a method of producing at least one recombinant product under aerated conditions. The method includes providing a porous substrate having a first side and a second side and which has a biofilm of microorganisms attached to the first side thereof, the substrate being configured to allow passage of a nutrient solution therethrough and to prevent the passage of microorganism cells therethrough. The method further includes causing a nutrient solution to flow through the substrate and the biofilm in a direction from the second side thereof to the first side thereof under aerated conditions, at a rate which is sufficiently low for a nutrient gradient having a concentration differential to be established across the biofilm, and the microorganisms being induced to produce recombinant product.01-21-2010
20100273214POLYPEPTIDES HAVING ALPHA-GLUCURONIDASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having alpha-glucuronidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.10-28-2010
20090275080Glucoamylase variants with altered properties - The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.11-05-2009
20090275079Novel genes encoding novel proteolytic enzymes - The invention relates to newly identified gene sequences that encode novel proteases obtainable from 11-05-2009
20110229931Engineering intracellular sialylation pathways - Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.09-22-2011
20090280527Amylase variants - The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant:11-12-2009
20100184137MODIFIED SECRETION SYSTEM TO INCREASE EXPRESSION OF POLYPEPTIDES IN BACTERIA - The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as 07-22-2010
20130122545Polypeptides Having Protease Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.05-16-2013
20100190207Expression Augmenting DNA Fragments, Use Thereof, and Methods for Finding Thereof - The invention provides recombinant DNA molecules comprising novel expression augmenting DNA fragments and an expression cassette, said expression cassette comprising a heterologous promoter linked to a nucleic acid of interest. The invention further provides uses of the novel expression augmenting DNA fragments. The invention further provides methods for obtaining novel expression augmenting DNA fragments.07-29-2010
20100173359ENHANCED BIOTHERAPEUTIC PRODUCTION USING INHIBITORY RNA - Compositions, kits, systems, equipment, and protocols utilize synthetic siRNA having a delivery facilitating moiety in improved bioprocesses that enhance the production of biomaterials. The siRNA can target genes associated with the following: 1) deleterious vector derived genes; 2) genes that confer non-optimal growth or differentiation properties to the cells; 3) genes that can influence heterogeneity or post-translational modification pattern of the desirable gene product; 4) genes that highly express non-desired proteins; 5) genes that express proteins which interfere with purification of the desired protein; and 6) other genes that can interfere with the bioprocess.07-08-2010
20100184140Compositions of Aminoacyl-tRNA Synthetase and Uses Thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.07-22-2010
20100184139BASB082 polynucleotides - The invention provides BASB082, BASB083, BASB091, BASB092 and BASB101 polypeptides and polynucleotides encoding BASB082, BASB083, BASB091, BASB092 and BASB101 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses.07-22-2010
20120270264DNA CONSTRUCT, AND PROCESS FOR PRODUCTION OF RECOMBINANT CHO CELL USING SAME - Disclosed is a DNA construct that is useful for efficient production of recombinant CHO cells useful for the production of target proteins. The DNA construct is a construct comprising, from a 5′ end toward a 3′ end, a first homologous DNA fragment, a target protein gene, and a second homologous DNA fragment. The first and second homologous DNA fragments have homology allowing for homologous recombination with a part of a hypoxanthine-phosphoribosyltransferase enzyme (hprt) locus in a CHO cell genome and have a chain length of not less than 1 kbp.10-25-2012
20100151520Translation Enhancer Elements Of Genes Encoding Human Tau Protein and Human Alpha-Synuclein Protein - The invention relates to translation enhancer elements that enhance translation of the gene encoding the human microtubule-associated tau protein and nucleic acid molecules that enhance translation of the gene encoding the human α-synuclein protein. The translation enhancer elements of the invention are useful in compositions and methods for identifying compounds for the prevention and/or treatment of neurodegenerative disease. The invention also includes in some aspects, vectors that include a translation enhancer element of the invention. The invention also includes the use of enhancer element containing vectors in methods to produce recombinant protein and in assays to identify compounds that modulate expression of tau protein or α-synuclein protein.06-17-2010
20100216187AMINO ACID SEQUENCES THAT BIND TO A DESIRED MOLECULE IN A CONDITIONAL MANNER - The present invention relates to amino acid sequences that bind to serum proteins such as serum albumin; to compounds, proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides, is essentially conditional on different physiological situations, e.g. is different under acidic condition than under pH-neutral condition.08-26-2010
20100196959ALLELES OF THE OPCA GENE FROM CORYNEFORM BACTERIA - The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.08-05-2010
20080213835Polypeptides Having Cellobiohydrolase II Activity And Polynucleotides Encoding Same - The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.09-04-2008
20110059487ENGINEERED MICROORGANISMS AND METHODS OF USE - The present invention provides, among other things, engineered microorganisms and methods that allow efficient conversion of soy carbohydrates to industrial chemicals by fermentation. In some embodiments, the invention provides microbial cells engineered to have increased efficiency in utilizing a soy carbon source (e.g., soy molasses, soy meal, and/or soy hulls) as compared to a parent cell. In some embodiments, microbial cells are engineered to have altered (e.g., increased) expression or activity of one or more carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, microbial cells are engineered to have altered localization of carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, engineered microbial cells provided herein are used to produce industrial chemicals (e.g., surfactin) using soy components as primary or sole carbon sources.03-10-2011
20110059486NON-SPLICING VARIANTS OF GP350/220 - Compositions comprising gp350 variant DNA and amino acid sequences are provided, as are vectors and host cells containing such sequences. Also provided is a process for producing homogeneous gp350 protein recombinantly and in the absence of production of gp220 protein, pharmaceutical compositions containing such protein and prophylactic treatments making use of such proteins.03-10-2011
20110059484Process for producing polypeptides - A process is described for producing a polypeptide heterologous to 03-10-2011
20100221778NOVEL LIGNIN-RESISTANT CELLULASE ENZYMES - Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.09-02-2010
20100151521Method for Stable Gene-Amplification in a Bacterial Host Cell - A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.06-17-2010
20100240097MAMMALIAN EXPRESSION VECTOR WITH A HIGHLY EFFICIENT SECRETORY SIGNAL SEQUENCE - The present invention relates to a mammalian cell based expression and secretion system and the expression and secretion of recombinant proteins by using secretory signal peptides. The present invention also relates to an expression cassette useful for the secretion of a heterologous gene from a mammalian cell, in particular a CHO cell. The present invention is also directed to a method of secreting a heterologous protein from mammalian cells such as CHO cells.09-23-2010
20100216186NOVEL SESQUITERPENE SYNTHASE GENE AND PROTEIN - The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.08-26-2010
20100216189SYNTHETIC REPETITIVE PROTEINS, THE PRODUCTION AND USE THEREOF - A repetitive protein having repetition units comprising the consensus sequence (I)08-26-2010
20100255534Recombinant Microorganism - Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a 10-07-2010
20100227361HOST CELL MODIFICATIONS THAT IMPROVE PEPTIDE PRODUCTION AND DOWNSTREAM PROCESSING - Disrupting the expression of endogenous 09-09-2010
20100221773Nucleic Acids Encoding a Functional Mammalin Purinoreceptor, P2X3, Methods of Production and Use Thereof - The subject invention relates to the rhesus monkey P09-02-2010
20100240096NOVEL HEMOPOIETIN RECEPTOR PROTEIN, NR10 - The inventors succeeded in isolating a novel hemopoietin receptor gene (NR10) using a sequence predicted from the extracted motif conserved in the amino acid sequences of known hemopoietin receptors. It was expected that two forms of NR10 exists, a transmembrane type and soluble form. Expression of the former type was detected in tissues containing hematopoietic cells. Thus, NR10 is a novel hemopoietin receptor molecule implicated in the regulation of the immune system and hematopoiesis in vivo. These novel receptors are useful in screening for novel hematopoietic factors capable of functionally binding to the receptor, or developing medicines to treat diseases related with the immune system or hematopoietic system.09-23-2010
20120142051TRANSCRIPTION REGULATORY FACTORS FOR MANNANASES OR CELLULASES, AND GENES FOR THE TRANSCRIPTION REGULATORY FACTOR - Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.06-07-2012
20100136620SOLUBLE, STABLE FORM OF HDM2, CRYSTALLINE FORMS THEREOF AND METHODS OF USE THEREOF - The present invention discloses modified Hdm2 proteins that are soluble. In addition, the present invention discloses nucleic acids that encode the modified Hdm2 proteins of the present invention. The invention also provides crystals of modified Hdm2 proteins that are suitable for X-ray crystallization analysis. The present invention also discloses methods of using the modified Hdm2 proteins and crystals thereof to identify, select and/or design compounds that may be used as anticancer agents. The present invention further discloses compounds that bind to modified Hdm2 proteins in protein-ligand complexes.06-03-2010
20090130710Enhancing vegetative protein production in transgenic plants using seed specific promoters - In various embodiments, the invention provides expression systems for heterologous protein expression in vegetative plant tissues, utilizing plant seed gene components that are adapted to orchestrate high levels of vegetative protein production. The expression systems may include host plant cells having recombinant genomes, and the plant cells may be maintained under protein expressing conditions, for example in tissue culture. The cells may be induced to express an ABD transcription factor, for example by transformation with a vector having a constitutive ABB expression cassette. The recombinant sequences in operative linkage may include an integrated expression promoter responsive to the ABI3 transcription factor, such as an arcelin gene promoter, a vicilin gene promoter and a napin gene promoter. A 5′ untranslated region may include a region of an ABA responsive plant seed gene or an AB 13 responsive plant seed gene. A plant secretion signal peptide coding sequence may be included. An integrated heterologous protein coding region, encoding a recombinant protein, may be provided in an open reading frame with the signal peptide coding sequence. A 3′ untranslated region may be provided having a polyadenylation signal.05-21-2009
20100136622Method for the Production of Proteins - The present invention relates to a process for the purification of a protease.06-03-2010
20100136617HALOHYDRIN EPOXIDASE - The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.06-03-2010
20100136616Selection of Host Cells Expressing Protein at High Levels - The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in said multicistronic transcription unit, and wherein an internal ribosome entry site (IRES) is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG startcodon. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, said host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention.06-03-2010
20090042248FOLDED RECOMBINANT CATALYTIC FRAGMENTS OF MULTIDOMAIN SERINE PROTEASES, PREPARATION AND USES THEREOF - The invention relates to unglycosylated folded C-terminal fragments of a multidomain serine protease of the complement cascade obtainable by expression in a bacterial host, wherein said serine protease is capable of binding a recognition molecule of the complement cascade, e.g. C1 or MBL. The invention also relates to methods and bacterial expression vectors for the preparation of said fragments, uses of said fragments for raising antibodies and screening substrates or inhibitors of said serine proteases and uses of the fragments in research and treatment of complement related disorders. The invention also relates to assay methods for assessing MASP-1 and MASP-2 levels in a sample of biological origin.02-12-2009
20090042247N-ACETYLGLUCOSAMINYLTRANSFERASE VB CODING SEQUENCES, RECOMBINANT CELLS AND METHODS - A previously unknown mammalian UDP-N-acetylglucosamine:α-6-D-mannoside β-1,6-N-acetylglucosaminyl-transferase (termed GlcNAc T-Vb herein) coding sequence, protein, recombinant host cells and antibodies which specifically bind GlcNAc T-Vb are described. In particular, GlcNAc T-Vb of mouse is disclosed.02-12-2009
20120196325LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING CARBOHYDRATE UTILIZATION-RELATED PROTEINS AND USES THEREFOR - Carbohydrate utilization-related and multidrug transporter nucleic acids and polypeptides, and fragments and variants thereof, are disclosed in the current invention. In addition, carbohydrate utilization-related and multidrug transporter fusion proteins, antigenic peptides, and anti-carbohydrate utilization-related and anti-multidrug transporter antibodies are encompassed. The invention also provides vectors containing a nucleic acid of the invention and cells into which the vector has been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.08-02-2012
20120196323Fermentation Process - The present invention relates to a fermentation process for culturing a host cell comprising a vector which comprises an inducible promoter of the mannose operon wherein the inducible promoter of the mannose operon controls expression of a nucleic acid sequence encoding for a polypeptide, in particular the present invention relates to the high cell density fermentation of a prokaryotic host cell comprising the inducible promoter of the mannose operon in the production of a polypeptide.08-02-2012
20120196322PROTEIN PRODUCTION - A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.08-02-2012
20090075337Novel protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof - A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into 03-19-2009
20090075333Reduced Genome E. Coli - Reduced genome strains of 03-19-2009
20090075330XYLANASES WITH ENHANCED THERMOPHILICITY AND ALKALOPHILICITY - The present invention provides a xylanase, or a modified xylanase enzyme comprising at least one substituted amino acid residue at a position selected from the group consisting of amino acid 11, 116, 118, 144 and 161, the position determined from sequence alignment of the modified xylanase with 03-19-2009
20090075335SELF-ASSEMBLING PROTEIN HYDROGEL WITH BIO-ACTIVE PROTEIN - Protein hydrogel monomers incorporating bio-active proteins and methods for producing the same are provided. In some embodiments, the disclosed subject matter includes a protein hydrogel monomer including a bio-active protein and two alpha helices that are adapted to interact with alpha helices on other monomers to form coiled-coil junctions.03-19-2009
20090075331Industrial-scale serum-free production of recombinant proteins in mammalian cells - The invention relates to methods for cultivating mammalian cells and for producing recombinant proteins in large-scale cultures of such cells. The proteins are, e.g., Factor VII or Factor VII-related polypeptides.03-19-2009
20120142049Recombinant Gene Expression - Genes are expressed by culturing cells comprising a host chromosome comprising an integrated artificial chromosome comprising recombinant genes, under conditions whereby each recombinant gene is expressed copy number dependently and position independently. Deletions increase expression from recombinant gene(s) inserted into the artificial chromosome.06-07-2012
20120034652Method for the Targeted Integration of Multiple Copies of a Gene of Interest in a Yarrowia Strain - This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a 02-09-2012
20120034651Akt activity specifically inhibiting polypeptide - The present invention is to provide a polypeptide specifically inhibiting the activity of Akt (Protein Kinase B), the DNA thereof, the antibody thereof, an inhibitor of Akt activity or an antitumor agent, and the like. The polypeptide comprises polypeptides (SEQ ID NO: 1, 3, 5, 7, and 9 of the sequence listing) that contain an amino acid sequence corresponding to any of the position of amino acid residue 10-24 of human TCL1, amino acid residue 8-22 of human TCL1B, amino acid residue 5-19 of human MTCP1, and amino acid residue 9-24 of mouse or rat TCL1; and the derivatives. Further, the present invention includes DNA encording the polypeptide (SEQ ID NO: 2, 4, 6, 8 or 10 of the sequence listing), and the antibodies specifically binding to the polypeptides. The polypeptide of the present invention can be used for an inhibitor of Akt activity, an antitumor agent, or the like.02-09-2012
20100196957CLONING AND CHARACTERIZATION OF 5' FLANKING REGIONS OF A HUMAN AGGRECANASE-1 GENE - The present invention relates to an isolated polynucleotide molecule comprising an Agg-1 promoter gene and methods of using the same. The invention also provides a method for producing an expression product encoded by a nucleic acid through use of an Agg-1 promoter.08-05-2010
20100221776Process for the Fermentative Production of Heterologous Proteins by Means of Escherichia Coli - A process for producing a heterologous protein is provided. The process comprises culturing an 09-02-2010
20100015661ANTIBODY-RNASE-CONJUGATE - The present invention provides a novel antibody-RNase conjugate which is a single chain protein, providing both the specificity of its antibody portion and the RNase activity of its RNase portion, resulting in an antigen specific effectiveness against cells when applied in vivo or in vitro, wherein the RNase portion is effectively cytotoxic in at least a fraction of cells presenting the antigen, e.g. after internalization by endocytosis. In detail, the present invention provides scAb-RNase conjugate having the principal structure of scFvFc-RNase. This structure could also be shown to allow the effective production of antigen-specific and cytotoxic conjugate protein in cell culture, the conjugate having a high activity with respect to antigen specificity and cyto01-21-2010
20090253169Use of genetically modified organisms to generate biomass degrading enzymes - The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.10-08-2009
20100196954Modified Endoglucanase II and Methods of Use - Described herein is a modified EGII cellulase having the amino acid sequence shown in SEQ ID NO:1, compositions comprising the modified EGII and methods of use.08-05-2010
20090275082Mast Cell-Derived Membrane Proteins - An originally developed efficient signal sequence trapping method was used to screen a cDNA library prepared from cultured mast cells derived from mouse bone marrow. As a result, genes encoding type I membrane proteins and comprising a single immunoglobulin domain in the extracellular domain and a motif for transmitting an inhibitory signal into cells were successfully isolated.11-05-2009
20100221780Modified Variant Bowman Birk Protease Inhibitors - The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.09-02-2010
20090221034Lipolytic Enzyme Variant With Improved Stability And Polynucleotides Encoding Same - The invention provides lipolytic enzyme variants having improved in-detergent stability and polynucleotides encoding same. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.09-03-2009
20090181428METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS - Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.07-16-2009
20090148890Nucleic acid and amino acid sequences relating to streptococcus pneumoniae for diagnostics and therapeutics - The invention provides isolated polypeptide and nucleic acid sequences derived from 06-11-2009
20090148891DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.06-11-2009
20080311620Novel Genes - The present invention is directed to novel genes mediating the carbon catabolite repression (CCR) of gluconeogenic genes. Furthermore, the polypeptides encoded by said genes as well as the use of said genes in a process for the production of a target fermentation product is provided. Processes for generating such microorganisms are also provided by the present invention. The invention is also related to a genetically engineered microorganism and its use for the production of a target fermentation product, wherein the gluconeogenic genes are relieved from CCR within said microorganism.12-18-2008
20080274497RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL - Methods and compositions for the production of recombinant proteins in a eukaryotic cell line are disclosed. The methods and compositions are particularly useful for generating stable expression of recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese hamster ovary cells.11-06-2008
20110129873Recombinant DNA Vectors for Expression of Human Prolactin Antagonists - Embodiments of the present invention relate generally to methods and compositions for producing human prolactin antagonists. Embodiments of the present invention also relate to various methods for improved production of human prolactin antagonists by microorganisms. These microorganisms, including 06-02-2011
20100304434Polypeptides having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.12-02-2010
20100304431tRNA SYNTHESIS METHOD, NUCLEIC ACID, AMINOACYL tRNA SYNTHESIS METHOD, AND PROCESS FOR PRODUCTION OF PROTEIN INTRODUCED WITH UNNATURAL AMINO ACID - The present invention relates to a process for producing a protein having an unnatural amino acid introduced therein, the process including: expressing in a eukaryotic cell an aminoacyl-tRNA synthetase, a nucleic acid having a sequence containing a eukaryote-derived tRNA nucleotide sequence linked to the 5′ end of a tRNA nucleotide sequence that is ligated with to an unnatural amino acid in the presence of the aminoacyl-tRNA synthetase, an unnatural amino acid, and a gene of a desired protein having a nonsense mutation at a predetermined position, to integrate the unnatural amino acid at the nonsense mutation position into the protein, thereby expressing a protein having an unnatural amino acid introduced therein.12-02-2010
20100311120HUMAN CYTOKINE RECEPTOR - Cytokines and their receptors have proven usefulness in both basic research and as therapeutics. The present invention provides polynucleotides, vectors, cells, and methods of production related to a new human cytokine receptor designated as “Zcytor16.”12-09-2010
20090053763Tumor endothelial marker 7-alpha molecules and uses thereof - The present invention provides Tumor Endothelial Marker 7α (TEM7α) polypeptides and nucleic acid molecules encoding the same. The invention also provides selective binding agents, vectors, host cells, and methods for producing TEM7α polypeptides. The invention further provides pharmaceutical compositions and methods for the diagnosis, treatment, amelioration, and/or prevention of diseases, disorders, and conditions associated with TEM7α polypeptides.02-26-2009
20100304435FLUORESCENT PROTEINS, THEIR PRODUCTION AND USE - The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.12-02-2010
20100317055Recombinant Cell Clones Having Increased Stability and Methods of Making and Using the Same - Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.12-16-2010
20110111459COMPOSITIONS AND METHODS FOR NUCLEIC ACID DELIVERY - Disclosed herein are methods and compositions for preparing a lipid encapsulated nucleocapsid delivery composition capable of delivering a nucleic acid to a mammalian cell. The nucleocapsid delivery compositions disclosed herein are useful for large-scale protein production, expression of genes in a mammalian expression system, and/or pharmaceutical production of a recombinant protein for treatment of an individual. In addition, the nucleocapsid delivery compositions can be used to treat an individual to replace a gene or gene product, or alternatively to inhibit a gene product using an RNA interference molecule.05-12-2011
20090035818Novel Polypeptide, Polynucleotide Encoding the Polypeptide and Use the Polypeptide and Polynucleotide - In one embodiment of the present application, a polypeptide capable of binding to a sugar chain is disclosed, particularly a high-mannose-type sugar chain bound to an antibody, more preferably a sugar chain bound to a chicken antibody. Also disclosed is a method for the purification of an antibody (specifically a chicken antibody) as a representative application of the polypeptide. Further disclosed is means for the purification. The polypeptide, BML-17, is a novel lectin made of 168 amino acid residues isolated from 02-05-2009
20110008836METHOD FOR PRODUCING ALPHA-SANTALENE - The present invention provides a method of producing α-santalene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). In particular, the method may be carried out in vitro or in vivo to produce α-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid represent part of the present invention. A non-human host organism and a cell transformed to be used in the method of producing α-santalene are also part of the present invention.01-13-2011
20110008831Scalable fermentation process - This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.01-13-2011
20110008835SYSTEM FOR THE HETEROLOGOUS EXPRESSION OF A VIRAL PROTEIN IN A CILIATE HOST CELL - The present invention relates to a system for the heterologous expression of a viral protein or a fragment thereof, said system comprising 01-13-2011
20110008832METHODS FOR STABLY RETAINING FOREIGN GENES IN CELLS - A method for preparing a protein or peptide encoded by a foreign gene by expressing a foreign gene, which comprises the steps of: preparing a recombinant vector comprising in an expressible state a gene encoding an aminoacyl-tRNA synthetase, in which a desired foreign gene has been inserted in an expressible state; preparing a mutant host cell or the like in which a chromosomal gene encoding an aminoacyl-tRNA synthetase has been knocked out; transforming the mutant host cell with the recombinant vector to obtain a transformant; and culturing the transformant to prepare the protein or peptide encoded by the foreign gene. It becomes possible to provide a novel means for permitting the retention of a recombinant DNA cloning vector without employing an antibiotic and without limiting the composition of the medium; and a method for preparing a protein or peptide encoded by a foreign gene by using the means.01-13-2011
20110008834POLYNUCLEOTIDES ENCODING ANTIBODIES DIRECTED AGAINST AMYLOID-BETA PEPTIDE - Monoclonal antibody 9TL and antibodies derived from 9TL directed against amyloid-beta peptide and methods of using same for diagnosing and treatment of Alzheimer's disease and Aβ peptide associated diseases are described. Methods of using antibodies directed against amyloid-beta peptide having impaired effector function for treatment of Alzheimer's disease and Aβ peptide associated diseases are also described.01-13-2011
20110033891MUSSEL BIOADHESIVE - The present invention relates to a bioadhesive derived from mussel. In particular, it relates to a novel 02-10-2011
20100081169PRODUCTION OF A LIPID ACYLTRANSFERASE FROM TRANSFORMED BACILLUS LICHENIFORMIS CELLS - The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a 04-01-2010
20100178672CHO CELL - The current invention comprises a CHO cell which is expressing a constitutively active mitogenic receptor, a method of obtaining such a CHO cell, and a method for the expression of a heterologous polypeptide using a CHO cell according to the invention.07-15-2010
20110244514Mammalian Sweet Taste Receptors - The present invention provides isolated nucleic acid and amino acid sequences of sweet taste receptors comprising two heterologous G-protein coupled receptor polypeptides from the T1R family of sensory G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet taste receptors.10-06-2011
20110053218MULTIPLE PROMOTER PLATFORM FOR PROTEIN PRODUCTION - A multiple promoter platform for expression of a protein or multiple and different proteins in a microorganism comprising two or more expression vectors having a nucleic acid molecule encoding a protein to be expressed, or having different nucleic acid molecules encoding different proteins to be expressed, wherein each vector has a different promoter operably linked to the nucleic acid molecule, and uses of the multiple promoter platform to produce recombinant proteins.03-03-2011
20090075336Variant Humicola Grisea CBH1.1 - Disclosed are variants of 03-19-2009
20110117599ARTIFICIAL ENTROPIC BRISTLE DOMAIN SEQUENCES AND THEIR USE IN RECOMBINANT PROTEIN PRODUCTION - Compositions and methods for recombinant protein production and, more particularly, fusion polypeptides, polynucleotides encoding fusion polypeptides, expression vectors, kits, and related methods for recombinant protein production.05-19-2011
20110020866Promoter sequence for the expression of recombinant proteins in Lactococcus lactis - The present invention relates to new DNA sequences that function as promoters, expression vectors containing such sequences, and host cells transformed with these vectors, in particular lactic acid bacteria such as 01-27-2011
20110086390Product and Process for Transformation of Thraustochytriales Microorganisms - Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.04-14-2011
20090081728PRODUCTION OF ATTENUATED NEGATIVE STRANDED RNA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES - Attenuated, recombinant negative stranded RNA viruses suitable for vaccine use are produced from one or more isolated polynucleotide molecules encoding the virus. A recombinant genome or antigenome of the subject virus is modified to encode a mutation within a recombinant protein of the virus at one or more amino acid positions(s) corresponding to a site of an attenuating mutation in a heretologous, mutant negative stranded RNA virus. A similar attenuating mutation as identified in the heterologous negative stranded RNA virus is thus incorporated at a corresponding site within the recombinant virus to confer an attenuated phenotype on the recombinant virus. The attenuating mutation incorporated in the recombinant virus may be identical or conservative in relation to the attenuating mutation identified in the heterologous, mutant virus. By the transfer of mutations into recombinant negative stranded RNA viruses in this matter, candidate vaccine viruses are engineered to elicit a desired immune response against a subject virus in a host susceptible to infection thereby.03-26-2009
20110244513ISOLATED MAMMALIAN MEMBRANE PROTEIN GENES; RELATED REAGENTS - Nucleic acids encoding various lymphocyte cell proteins from mammalian, including primate, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided.10-06-2011
20090253178RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME - Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.10-08-2009
20100015664PROTEASE, DNA ENCODING THE SAME, AND METHOD FOR MANUFACTURING PROTEASE - A protease having the following characteristics: 01-21-2010
20110129872METHOD FOR A PRODUCTION OF A RECOMBINANT PROTEIN USING YEAST CO-EXPRESSION SYSTEM - The present invention relates to a method for mass production of a recombinant protein comprising the step of culturing a yeast transformed with: a recombinant gene construct comprising a yeast promoter, a gene coding a signal sequence and a gene coding a target protein; and also with one or more genes coding folding accessory protein selected from the group consisting of PDI1 (protein disulfide isomerase 1), SEC23 (secretory 23), TRX2 (thioredoxin 2) AHA1 (activator of heat shock protein 90 ATPase), and SCJ1 (06-02-2011
20100021964MICROORGANISM FOR PRODUCING RECOMBINANT PIG LIVER ESTERASE - The present invention relates to a microorganism which comprises at least one copy of a polynucleic acid sequence which is foreign to the host and which encodes a protein having an enzymic activity, and comprises a chaperone system which assists the expression of the protein in the form of an active enzyme, and to a method for producing a protein having esterase activity using such a microorganism.01-28-2010
20090311746INTERGENIC REGIONS AS INSERTION SITES IN THE GENOME OF MODIFIED VACCINIA VIRUS ANKARA (MVA) - The present invention relates to novel insertion sites useful for the integration of exogenous sequences into the Modified Vaccinia Ankara (MVA) virus genome. The present invention further provides plasmid vectors to insert exogenous DNA into the genome of MVA. Furthermore, the present invention provides recombinant MVA comprising an exogenous DNA sequence inserted into said new insertion site as medicine or vaccine.12-17-2009
20090311743Process for the Production, in Prokaryotes, of Active, Stable Transposases of Mariner Mobile Genetic Elements - The present invention relates to a process for the production, by a prokaryotic host cell, of an active, stable transposase of a Mariner mobile genetic element belonging to the 12-17-2009
20100227364Novel Lipolytic Enzyme ELIP - The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.09-09-2010
20100221777DESIGNER LIGANDS OF TGF-BETA SUPERFAMILY - The present disclosure relates to chimeric polypeptide having TGF-beta activity, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.09-02-2010
20100221779PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of 09-02-2010
20110086391Expression Vector - An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.04-14-2011
20110086387MUTANT NANOARCHAEUM EQUITANS A523R DNA POLYMERASE AND ITS USE - Disclosed herein is a mutant Neq A523R DNA polymerase consisting of an amino acid sequence of SEQ ID NO: 2 wherein alanine at amino acid position 523 in a Nanoarchaeum equitans DNA polymerase (Neq DNA polymerase) consisting of an amino acid sequence of SEQ ID NO: 1 has been substituted with arginine by site-directed mutagenesis. Also disclosed are a gene consisting of a nucleotide sequence encoding the mutant Neq A523R DNA polymerase, a recombinant vector comprising the gene, and a transformant transformed with the vector. In addition, disclosed are a PCR kit comprising the mutant Neq A523R DNA polymerase having excellent performance compared to the wild-type Neq DNA polymerase, and a method for preparing the Neq A523R DNA polymerase.04-14-2011
20100112637Polypeptides Having Alpha-Amylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.05-06-2010
20100021963Genetic incorporation of unnatural amino acids into proteins in mammalian cells - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.01-28-2010
20110244515METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID - The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.10-06-2011
20110086389Compositions and Methods for Expressing In-Frame Multimeric Proteins - The present invention concerns methods and compositions for making and using multimeric, single chain proteins of a defined structure. In various embodiments the invention involves a bank or collection of one or more monomer nucleic acid species with each such nucleic acid species encoding a protein monomer subunit species and divided into separate subspecies, each bearing a distinct pair of position-specific subcloning restriction endonuclease recognition sites for cassette style cloning into an expression vector and making multimeric, single chain proteins of a defined structure.04-14-2011
20110250641ZWITTERIONIC ACID-LABILE SURFACTANTS AND METHODS OF USE - A compound of the formula:10-13-2011
20090209002COMPOSITIONS AND METHODS RELATING TO CELLULAR TARGETING - Plasmodesmal resident components are identified, characterized and isolated. Compositions comprising these components are described and methods of use thereof for plasmodesmal flux modulation and targeting are enabled. In a first embodiment, a novel plasmodesmal receptor-like protein, referred to herein as pldp1, reveals signals sufficient for targeting to plasmodesmata via the secretory pathway. In the second embodiment a novel plasmodesmal protein that is anchor into the external face of the plasma membrane and binds to callose is provided.08-20-2009
20090311748HEART20049410 POLYPEPTIDES AND METHODS OF MAKING THE SAME - Novel full-length cDNAs are provided.12-17-2009
20100055740PRODUCTION OF CELLULASE ENZYMES IN PLANT HOSTS USING TRANSIENT AGROINFILTRATION - Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an 03-04-2010
20100062489DUCK EMBRYONIC DERIVED STEM CELL LINES FOR THE PRODUCTION OF VIRAL VACCINES - The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from duck embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.03-11-2010
20100055739Methods of improving the introduction of DNA into bacterial cells - The present invention relates to methods of improving the introduction of DNA into bacterial host cells.03-04-2010
20100062486NOVEL PROTEIN AND DNA ENCODING THE PROTEIN - The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).03-11-2010
20100062488PREPARATION PROCESS OF RECOMBINANT HUMAN P43 PROTEIN - A process for producing human p43 protein is provided, in which the p43 protein can be used as a drug for treating solid tumor.03-11-2010
20100062484Recombinant n-glycosylated proteins from procaryotic cells - The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.03-11-2010
20110250640METHOD OF RECLONING PRODUCTION CELLS - A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.10-13-2011
20090215118D-AMINOACYLASE - A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost.08-27-2009
20090215117Site specific incorporation of keto amino acids into proteins - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.08-27-2009
20090215115SIALYLTRANSFERASES COMPRISING CONSERVED SEQUENCE MOTIFS - The present invention provides, e.g., sialyltransferase proteins comprising conserved sequence motifs, including α-2,3-sialyltransferase proteins from 08-27-2009
20090215114METHOD OF ENZYMATICALLY SYNTHESIZING 3' -PHOSPHOADENOSINE-5' -PHOSPHOSULFATE - The invention provides a method for producing 3′-phosphoadenosine 5′-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5′-triphosphate sulfurylase (ATPS) and adenosine 5′-phosphosulfate kinase (APSK), wherein an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.08-27-2009
20110151512OLIGOPEPTIDE-FREE CELL CULTURE MEDIA - The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.06-23-2011
20110250639METHODS FOR PRODUCING SOLUBLE MEMBRANE-SPANNING PROTEINS - Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.10-13-2011
20110076721EFFICIENT PRODUCTION OF HETEROLOGOUS PROTEINS USING MANNOSYL TRANSFERASE INHIBITORS - Compounds and methods are described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of certain benzylidene thiazolidine-diones inhibitors of Pmt-mediated O-linked glycosylation.03-31-2011
20110081680Animal Protein-Free Media For Cultivation of Cells - The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.04-07-2011
20110104751VARIANT FORM OF URATE OXIDASE AND USE THEREOF - The present invention relates to genetically modified proteins with uricolytic activity. More specifically, the invention relates to proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidases.05-05-2011
20110076720NICKING ENDONUCLEASE METHODS AND COMPOSITIONS - A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or gutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6.03-31-2011
20110070610Processing Enzymes Fused to Basic Protein Tags - The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.03-24-2011
20110070609Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems - The present invention provides systems and methods for producing a nucleic acid or protein in transgenic sprouted seedlings or sprouted seedlings engineered to transiently express a nucleic acid or protein of interest. The sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter or a viral promoter. The sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the nucleic acid or protein.03-24-2011
20120149060Nucleic Acids for Cloning and Expressing Multiprotein Complexes - The present invention relates to a nucleic acid containing at least one homing endonuclease site (HE) and at least one restriction enzyme site (X) wherein the HE and X sites are selected such that HE and X result in compatible cohesive ends when cut by the homing endonuclease and restriction enzyme, respectively, and the ligation product of HE and X cohesive ends can neither be cleaved by the homing endonuclease nor by the restriction enzyme. Further subject-matter of the present invention relates to a vector comprising the nucleic acid of the present invention, host cells containing the nucleic acid and/or the vector, a kit for cloning and/or expression of multiprotein complexes making use of the vector and the host cells, a method for producing a vector containing multiple expression cassettes, and a method for producing multiprotein complexes. The invention also relates to a methods of assembling multiple single vectors (“vector entities”) into fusion vectors and to method of disassembling a fusion vector containing multiple of such vector entities into single vectors. The invention is also directed to fusion vectors containing multiple vector entities.06-14-2012
20110033892NOVEL MEMBERS OF THE CAPSAICIN/VANILLOID RECEPTOR FAMILY OF PROTEINS AND USES THEREOF - The invention provides isolated nucleic acids molecules, designated hVR-1, hVR-2, and rVR-2 nucleic acid molecules, which encode novel members of the Capsaicin/Vanilloid receptor family. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing hVR-1, hVR-2, and rVR-2 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an hVR-1, hVR-2, and rVR-2 gene has been introduced or disrupted. The invention still further provides isolated hVR-1, hVR-2, and rVR-2 proteins, fusion proteins, antigenic peptides and anti-hVR-1, anti-hVR-2, and anti-rVR-2 antibodies. Diagnostic methods utilizing compositions of the invention are also provided.02-10-2011
20100311118PLASMID-ENCODED NEUROTOXIN GENES IN CLOSTRIDIUM BOTULINUM SEROTYPE A SUBTYPES - The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique 12-09-2010
20100297694NOVEL GENE FROM HANCENULA POLYMORPHA CAPABLE OF CONTROLLING UNFOLDED PROTEIN RESPONSE AND METHOD FOR INCREASING EFFECT OF SECRETION USING THE SAME - The present invention relates to a novel 11-25-2010
20110008833PROTEIN PRODUCTION IN PLANT CELLS AND ASSOCIATED METHODS AND COMPOSITIONS - The present invention relates to methods and compositions for the expression of protein. Embodiments of methods may comprise the cleavage and repair of a nucleotide sequence encoding a highly expressed protein leading to a reduction in the expression of the highly expressed protein.01-13-2011
20100311117NUCLEIC ACIDS ISOLATED FROM THE INTESTINE - A recombinant intestinal nucleic acid is provided. One embodiment of the present invention contemplates the use of a gut-specific promoter, wherein a promoter can be the chicken intestinal fatty acid binding protein promoter region. A method for making a transgenic bird is also disclosed by transfecting a bird with a vector comprising a recombinant nucleic acid comprising a chicken intestinal fatty acid binding protein promoter region operably linked to a heterologous nucleic acid expressing a desired polypeptide to be expressed in the gut tissue of an avian.12-09-2010
20100311119Single-domain antigen-binding proteins that bind mammalian IgG - The present application relates to antigen-binding proteins that are capable of binding to mammalian IgG. The frame-work regions of the antigen-binding proteins of the application preferably correspond to those of antibodies naturally that are devoid of light chains as may e.g. be found in camelids. The application further relates to nucleic acids that encode such antigen-binding proteins, to immunoadsorbent materials that comprise such proteins, to the uses of such immunoadsorbent materials for the purification of mammalian IgG antibodies and for therapeutic apheresis.12-09-2010
20100304433Polypeptides Having Protease Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.12-02-2010
20100297695Nucleic Acids Encoding Chimeric CD154 Polypeptides - The present invention provides for an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor. The present invention also provides for the chimeric CD154 that is encoded by the above-described polynucleotide sequence, an expression vector and a genetic vector comprising the polynucleotide sequence, a host cell comprising the expression vector or the genetic vector, a process for producing the chimeric CD154, and methods for utilizing the expression vectors and genetic constructs containing the chimeric CD154 polynucleotide sequences.11-25-2010
20100297696Enzyme Production in Culture Medium Comprising Raw Glycerol - The present invention is directed to a method of producing desired proteins from a host cell grown in a media comprising raw glycerol as a carbon source.11-25-2010
20110151511RAPID GROWING MICROORGANISMS FOR BIOTECHNOLOGY APPLICATIONS - The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.06-23-2011
20100255535NOVEL GENE UPREGULATED IN CANCERS OF THE PROSTATE - The present invention relates to a novel protein designated 20P2H8 which shares homology with several heterogeneous nuclear ribonucleoproteins (hnRNPs). A full length approximately 3600 by 20P2H8 cDNA (SEQ ID NO: 1, encoding a 517 amino acid open reading frame (SEQ ID NO: 2), is provided herein.10-07-2010
20100255536HIGH PRESSURE REFOLDING OF PROTEIN AGGREGATES AND INCLUSION BODIES - The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.10-07-2010
20080248525Twin-Arginine translocation in bacillus - Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a 10-09-2008
20080248523Utilisation of Constructs Comprising Recombination Sequence Motifs for Enhancing Gene Expression in Moss - A method of amplifying gene expression in a moss plant cell or moss tissue, DNA constructs therefor, moss plant cells and uses thereof for the production of protein.10-09-2008
20080248524AMP Deaminase Originating Streptomyces And Utilization Thereof - It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H10-09-2008
20100068759MODIFIED CHONDROITIN SYNTHASE POLYPEPTIDE AND CRYSTAL THEREOF - Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.03-18-2010
20100003720ENZYME DERIVED FROM THERMOPHILIC ORGANISMS THAT FUNCTIONS AS A CHROMOSOMAL REPLICASE, AND PREPARATION AND USES THEREOF - A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in 01-07-2010
20090317865Eubacterial RNA-Polymerase Mutants With Altered Product Production - The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.12-24-2009
20090317864Alpha-Amylase Mutants - The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca12-24-2009
20090291469Compositions and Methods for Producing Fermentation Products and Residuals - The present invention provides compositions and methods designed to increase value output of a fermentation reaction that yields a first product, intended for commercialization, such as ethanol, and a fermentation residual used, for example, as animal feed. The methods involve using microorganisms in the fermentation process that have been modified so as to yield a residual having greater value that a residual produced in the process by a microorganism not so modified. In particular, the present invention contemplates using microorganisms in a fermentation process that have been modified to increase production of a nutrient, such as an essential amino acid, thereby reducing the need to supplement the nutrient in the animal's diet. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.11-26-2009
20090253175Soluble hyaluronidase glycoprotein (sHASEGP), process for preparing the same, uses and pharmaceutical compositions comprising thereof - Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.10-08-2009
20090186379ERK ligands and polynucleotides encoding ERK ligands - The invention relates to kinase inhibitor ligands and polyligands. In particular, the invention relates to ligands and polyligands that modulate ERK activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands and polyligands to a cellular localization signal, epitope tag and/or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.07-23-2009
20110256586Methods and Compositions for the Prevention and Treatment of Anemia - Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs.10-20-2011
20090111142Method for manufacturing a recombinant polyclonal protein - The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.04-30-2009
20090136998LUCIFERASES AND METHODS FOR MAKING AND USING THE SAME - Briefly described, embodiments of this disclosure include polynucleotides that encode mutant 05-28-2009
20080318271Subtilases - The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.12-25-2008
20080213829Materials and methods to increase peptide chain expression - DNA sequences that increase peptide chain expression when operably linked to a gene encoding the peptide chain and methods of generating a peptide chain expression host cell using the foregoing are disclosed. Peptide chain expression host cells are also disclosed.09-04-2008
20080213832SESQUITERPENE SYNTHASES AND METHODS OF USE - The invention relates to sesquiterpene synthases and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthases. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various oxygenated and aliphatic sesquiterpenes including valencene, bicyclo-germacrene, cubebol and delta-cadinene.09-04-2008
20080213839Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides - The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing messes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.09-04-2008
20080213838DNA, vector, transformant and method for producing APA protein - It is intended to provide a production method by which human APA protein can be recovered more efficiently and in a larger quantity compared to a conventional method; DNA encoding a recombinant human APA protein which is necessary when the human APA protein is produced; a vector in which the DNA is integrated; and a transformant which retains the vector.09-04-2008
20080254507Anti-Hiv Drug, Polypeptide Constituting the Same, Gene Encoding the Polypeptide and Method of Producing the Anti-Hiv Drug - It is intended to provide an anti-HIV drug characterized by containing multimeric actinohivin, a polypeptide which is multimeric actinohivin; a gene encoding the same; and a method of producing the anti-HIV drug. The anti-HIV drug inhibits the synctium formation and has an enhanced effect10-16-2008
20080254509NUCLEOTIDYL TRANSFERASES WITH ENHANCED NUCLEOTIDE TRIPHOSPHATE FLEXIBILITY - The present invention provides mutant Rm1A enzymes possessing an increased purine/pyrimidine bias in nucleotide triphosphate substrate specificity as compared to a corresponding non-mutated Rm1A enzyme. Such enzymes expand the types of substrates that can be used in enzymatic glycorandomization methods thereby increasing diversity of chemical libraries.10-16-2008
20080254508TYPE I POLYKETIDE SYNTHASE EXTENDER UNITS - Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.10-16-2008
20080254510NUCLEIC ACID ENCODING PROTEINS INVOLVED IN PROTEIN DEGRADATION, PRODUCTS AND METHODS RELATED THERETO - In accordance with the present invention, there are provided novel Siah-Mediated-Degradation-Proteins (SMDPs) and/or SCF-Complex Proteins (SCPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SMDPs and/or SCPs can be employed in a variety of ways, for example, for the production of anti-SMDP and/or SCP antibodies thereto, in therapeutic compositions, and methods employing such proteins and/or antibodies for drug screening, functional genomics and other applications. Also provided are transgenic non-human mammals that express the invention protein.10-16-2008
20100279349NUCLEIC ACIDS ENCODING TWO-COMPONENT SENSING AND REGULATORY PROTEINS, ANTIMICROBIAL PROTEINS AND USES THEREFOR - Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells into which the expression vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.11-04-2010
20120202246Vector Comprising Mannose Promoter and Mannose Promoter - The present invention relates to a vector expressible in a prokaryotic host and a nucleic acid sequence comprising a mannose-inducible promoter of the mannose operon of 08-09-2012
20120202245Method for Producing Natively Folded Proteins in a Prokaryotic Host - The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.08-09-2012
20120202244Antibodies Capable of Specifically Binding to a Specific Amino Acid Sequence - The present invention provides for an antibody or fragment thereof capable of specifically binding to an epitope of the amino acid sequence CDPAFLYKVVD (SEQ ID NO:1) or a fragment of at least 5, 6, or 7 amino acids thereof.08-09-2012
20110053220NOVEL PROTEIN CAPABLE OF BINDING TO HYALURONIC ACID, AND METHOD FOR MEASUREMENT OF HYALURONIC ACID USING THE SAME - The present invention relates to a polynucleotide encoding a protein comprising an amino acid sequence shown in SEQ ID NO: 2, wherein the protein encoded by the polynucleotide has a hyaluronic acid binding ability, the protein, a method for measuring hyaluronic acid using the protein, and a reagent kit for measuring hyaluronic acid comprising the protein as a constituent.03-03-2011
20110053219Method for the Targeted Integration of Multiple Copies of a Gene of Interest in a Yarrowia Strain - This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a 03-03-2011
20110053217Polynucleotides encoding signal peptide-containing molecules - The invention provides human signal peptide-containing proteins (HSPP) and polynucleotides which identify and encode HSPP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with expression of HSPP.03-03-2011
20110053216Modified Cyanobacteria - Disclosed is a modified photoautotrophic bacterium comprising genes of interest that are modified in terms of their expression and/or coding region sequence, wherein modification of the genes of interest increases production of a desired product in the bacterium relative to the amount of the desired product production in a photoautotrophic bacterium that is not modified with respect to the genes of interest.03-03-2011
20100285528METHOD, VECTOR AND SYSTEM FOR EXPRESSING POLYPEPTIDES - Disclosed herein are methods and systems for expressing a polypeptide in a 11-11-2010
20100285529FELINE GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR PROTEINS - The present invention relates to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins; to canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD 154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF nucleic acid molecules, including those that encode canine interleukin-4, canine or feline Flt-3 ligand, canine or feline CD40, canine or feline CD 154, canine interleukin-5, canine interleukin-13, feline interferon alpha, and/or feline GM-CSF proteins, respectively; to antibodies raised against such proteins; and to inhibitory compounds that regulate such proteins. The present invention also includes methods to identify and obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to regulate an immune response in an animal.11-11-2010
20110136169EXPRESSION SYSTEM COMPOSITIONS AND METHODS - The present invention relates to novel expression systems and methods for preparing samples for 3D structure determination of a protein based on a protein expression vector, 06-09-2011
20100304432GENETICALLY MODIFIED BIOLOGICAL CELLS - The present invention is based, in part, on our discovery of a way to configure expression cassettes so that the expression of a selectable marker protein, which is critical for the growth or survival of a cell, also results in the expression of a protein of interest in a biological cell. Accordingly, in one aspect, the invention features a genetically modified cell (e.g., a bacterial cell) that includes a chromosomally integrated or cytoplasmic expression cassette that includes a first nucleic acid sequence encoding a protein of interest and a second nucleic acid sequence encoding a selectable marker protein. The regulatory sequence (e.g., the sequence encoding a functional promoter) that drives expression of the required selectable marker protein also drives expression of the protein of interest. For that reason, we may refer to their expression as being “linked” or “functionally couple.”12-02-2010
20100184136Methods For Enhancing A Secretion Efficiency Of Recombinant Foreign Protein In Yeast Expression System - Provided is a method for improving secretion efficiency of a recombinant foreign protein in a yeast expression system. The method comprises transforming a yeast host with a recombinant foreign gene construct comprising a galactose-inducible promoter, a secretion signal sequence and a gene encoding the foreign protein to construct a transformed yeast strain; and culturing the transformed yeast strain under the condition that the activity of the galactose-inducible promoter is controlled. Improved secretion efficiency of the foreign protein can be achieved by decreasing over-expression-induced insoluble precipitation of the recombinant foreign protein suffered by a conventional galactose-inducible promoter-based yeast expression system, via appropriate control of a level of galactose functioning as an inducer of the galactose-inducible promoter in cells. Due to improved secretion efficiency of the recombinant foreign protein, present invention makes a contribution to improvement in productivity of recombinant foreign proteins in the yeast expression system and reduction in production costs.07-22-2010
20100184138Heterologous and Homologous Cellulase Expression System - The present invention provides filamentous fungi that express a combination of heterologous and homologous polypeptides, polypeptide mixtures comprising a combination of heterologous and homologous polypeptides and methods of producing the polypeptide mixtures.07-22-2010
20110086388CHAPERONE-ASSISTED PROTEIN EXPRESSION AND METHODS OF USE - The present disclosure provides methods of utilizing chaperone proteins for the production of active protein such as those encoded by the genes of natural biosynthetic clusters. The methods provided herein have applicability for a wide variety of genes ranging from small fatty acid biosynthetic genes to large non-ribosomal peptide synthetase genes.04-14-2011
20100323399Use of Trail Polypeptides to Treat Cancer - A novel cytokine designated TRAIL induces apoptosis of certain target cells, including cancer cells and virally infected cells. Isolated DNA sequences encoding TRAIL are disclosed, along with expression vectors and transformed host cells useful in producing TRAIL polypeptides. Antibodies that specifically bind TRAIL are provided as well.12-23-2010
20100323397MODIFIED VIRD2 PROTEIN AND ITS USE IN IMPROVED GENE TRANSFER - There is provided a method for 12-23-2010
20100323398Baculoviruses With Enhanced Virion Production and a Method for the Production of Baculoviruses - The present invention provides a method for restoring budding capability to GP64null baculoviruses including gp64null AcMNPV by expressing therein a portion of the VSV G protein gene or a truncated “stem” portion of the GP64 gene. Other embodiments provide methods to use portions of the G-stem or GP64 protein to target foreign proteins for display on virions.12-23-2010
20100323400Compositions and Methods for Controlling Copy Number for a Broad Range of Plasmids and Uses Thereof - The present invention provides compositions and methods for controlling the copy number for a broad range of plasmids and uses thereof. Disclosed is a host cell for conditional control of copy number of a plasmid, which host cell comprises a poly(A) polymerase gene that is operably joined to a conditionally inducible promoter, and a method for cloning and stably maintaining a DNA sequence encoding a heterologous polypeptide in the host cell.12-23-2010
20100330617Methods of Producing a Secreted Protein - The invention is directed to methods of producing a polypeptide or a variant thereof, wherein the polypeptide or variant thereof is dependent on LIMP-2 for trafficking, localization, stabilization and/or sorting of the polypeptide in the cell. In general, the methods comprise culturing a lysosomal integral membrane protein II (LIMP-2) deficient cell which expresses the polypeptide or the variant thereof under conditions in which the polypeptide or the variant thereof is produced.12-30-2010
20100330616NOVEL ENDORIBONUCLEASE - A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.12-30-2010
20100330618Methods for producing biological substances in pigment-deficient mutants of bacillus cells - The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent 12-30-2010
20110136170METHOD AND KIT FOR PURIFICATION OF RECOMBINANT PROTEINS USING A SELF-CLEAVING PROTEIN INTEIN - The present invention provides a method for purifying a recombinant target protein by using an autolytic protein Intein, comprising: recombinantly expressing a fusion protein in a host cell, wherein said fusion protein comprises a target protein domain, an autolytic Intein and one or multiple hydrophobic granule binding domains, and wherein said autolytic Intein is located between said target protein domain and said one or multiple hydrophobic granule binding domains; releasing said fusion protein from said host cell, adding hydrophobic granules and incubating; collecting the incubated hydrophobic granules, adding a lysis solution for allowing the autolytic Intein in said fusion protein to disrupt; and removing said hydrophobic granules, to obtain a solution containing said target proteins which are substantially purified. The present invention further provides a kit for performing such a method.06-09-2011
20110136171IMPROVED PROTEIN EXPRESSION SYSTEM - Disclosed is a DNA polynucleotide comprising a nucleic acid sequence having promoter activity in a 06-09-2011
20110129874Pichia Pastoris Das Promoter Variants - The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from 06-02-2011
20100203587USE OF DNA GYRASE INHIBITORS FOR IN VITRO POLYPEPTIDE SYNTHESIS REACTIONS - The present invention provides methods and compositions useful for in vitro polypeptide synthesis reactions. The methods involve the use of DNA gyrase inhibitors to prevent bacterial contamination in lysates used for in vitro production of polypeptides. The compositions include contamination-free cell lysates for in vitro protein synthesis reactions.08-12-2010
20100159511PRODUCTION OF RECOMBINANT SELENOPROTEIN MUTANTS WITH ENHANCED CATALYTIC ACTIVITY - The present invention generally relates to the production of industrially relevant quantities of selenoprotein enzymes in eukaryotic cell cultures. More specifically, the present invention generally relates to the production of such enzymes wherein one or more catalytic cysteine or serine residues are mutagenically replaced by selenocysteine.06-24-2010
20090197303PROCESS FOR PRODUCING DIPEPTIDES - The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.08-06-2009
20120208236METHODS FOR DETECTING MODULATORS OF CYTOKINE RECEPTOR ZALPHA11 - Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zalpha11, a novel cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding receptor polypeptides can also be used to block ligand activity in vitro and in vivo. The polynucleotides encoding zalpha11, are located on chromosome 16, and can be used to identify a region of the genome associated with human disease states. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.08-16-2012
20090203071EXPRESSION IN INSECT CELLS OF GENES WITH OVERLAPPING OPEN READING FRAMES, METHODS AND COMPOSITIONS THEREFOR - The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.08-13-2009
20090197302Method for Producing an L-Amino Acid Using a Bacterium of the Enterobacteriaceae Family Having Attenuated Expression of the nac Gene - The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus 08-06-2009
20090176275EXPRESSION SYSTEM, COMPONENTS THEREOF AND METHODS OF USE - Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from 07-09-2009
20090176274GLYCOSYLATED MAMMALIAN NGAL AND USE THEREOF - The present invention relates to glycosylated mammalian NGAL, and methods of using said glycosylated mammalian NGAL.07-09-2009
20090176273Production of Lipidated Proteins In E. coli - Production of a lipidated protein in an 07-09-2009
20090176272EXPRESSION OF NUCLEIC ACID SEQUENCES FOR PRODUCTION OF BIOFUELS AND OTHER PRODUCTS IN ALGAE AND CYANOBACTERIA - Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain plastid-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, pharmaceuticals or fine chemicals.07-09-2009
20110189729NOVEL RECOMBINATION SEQUENCES - The present invention relates to novel nucleotide sequences, which are variants of att recombination sequences, involved in sequence-specific recombination of DNA in eukaryotic cells, whereby sequence specific recombination is performed by a bacteriophage lambda integrase Int. Such novel att recombination sequences being e.g. attP.b, attP.a, attL.a, attR.a and attR.b. The present invention further relates to a method of sequence-specific recombination of DNA in eukaryotic cells, comprising the introduction of a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int, whereby at least one of said first or second DNAs is a novel att recombination sequences being e.g. attP.b, attP.a, attL.a, attR.a and attR.b.08-04-2011
20100190208ACID FUNGAL PROTEASES - The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.07-29-2010
20120309050SYSTEM FOR INCREASING GENE EXPRESSION AND VECTOR COMPRISING THE SYSTEM - An object of the present invention is to provide a method for increasing the expression of foreign genes, in particular, using a promoter, an enhancer, and the like, and an expression cassette containing a promoter, an enhancer, and the like, by which gene expression can be increased. The purpose is achieved with the use of the gene expression cassette comprising a DNA construct containing a gene to be expressed and a poly A addition sequence that are located downstream of a 112-06-2012
20120309051COMPOSITIONS AND METHODS FOR PREPARING RECOMBINANT MG53 AND METHODS FOR OPTIMIZING SAME - Disclosed herein are nucleic acid sequences that encode novel polypeptides. In particular, the present invention provides nucleic acid molecules that include optimization features that enhance the expression and/or recovery and/or activity of encoded polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.12-06-2012
20110189731POLYNUCLEOTIDES ENCODING RECOMBINANT LUBRICIN MOLECULES AND USES THEREOF - Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.08-04-2011
20110189730VIRAL NUCLEOCAPSID PROTEIN AS A MULTIFUNCTIONAL TRANSLATION INITIATION FACTOR AND INCREASED PROTEIN AND POLYPEPTIDE PRODUCTION USING SAME - The present invention is directed to a system to significantly increase the expression of genes of interest, and in particular proteins and polypeptide products. Expression of hantavirus nucleocapsid protein (N) by itself results in augmented translational expression of diverse genes. The mechanism of this augmentation relies on the ability of N to replace the cellular cap binding complex to attain more efficient translation initiation—the result being great mRNA production and greater protein/polypeptide production. The inventors have also recently found that inclusion of a 5′ untranslated leader region (viral UTR) from a viral RNA, in conjunction with N, leads to even more robust expression. This mechanism appears to involve recognition of the viral UTR by the N to provide even more robust protein production. Thus, a general strategy for expression of any gene would be to generate significant quantities of mRNA containing the viral UTR from a strong promoter, and then to allow translation of mRNA of a gene product in the presence of N. Even a modest increase in the production of commercially desirable proteins is a goal in industry.08-04-2011
20100028943Modified Messenger RNA Stabilizing Sequences for Expressing Genes in Bacterial Cells - The present invention relates to methods of producing a polypeptide having biological activity in a bacterial cell, comprising: (a) cultivating a bacterial host cell in a medium conducive for production of the polypeptide, wherein the bacterial host cell comprises a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide, wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium. The present invention also relates to such modified mRNA processing/stabilizing sequences, nucleic acid constructs, and bacterial host cells and to methods of obtaining such bacterial host cells.02-04-2010
20100028942Methods for Improving Viability and Productivity in Cell Culture - Methods for increasing viability and production of secreted proteins in fed batch eukaryotic cell culture are disclosed.02-04-2010
20100028940Compositions and Methods for Metabolic Selection of Transfected Cells - The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media.02-04-2010
20080274502Bacillus Thuringiensis CryET33 and CryET34 Compositions and Uses Therefor - Disclosed are 11-06-2008
20120040399METHOD FOR THE PRODUCTION OF RECOMBINANT POLYOMAVIRAL VECTOR PARTICLES - The present invention relates to improved methods for the production of viral particles, viral vector particles and recombinant proteins. In particular, the invention relates to improved methods for the production of recombinant polyomaviral vector particles and polyomaviral vector production cell lines. More in particular, the invention relates to methods for the production of simian polyomaviral vector particles such as simian virus 40 (SV40) viral vector particles. The invention also relates to compositions comprising viral vectors and uses thereof and viral vector particles to treat genetic disorders, transplant rejection, autoimmune diseases, infectious diseases, allergies or cancer. The invention also relates to methods for the production of recombinant proteins in mammalian cells and methods to enhance the production of recombinant proteins in mammalian cells.02-16-2012
20100196958Reduced Phosphotransferase System Activity in Bacteria - A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.08-05-2010
20110111457METHODS FOR MICROBIAL PRODUCTION OF TERPENOIDS - The invention relates to recombinant expression of terpenoid synthase enzymes and geranylgeranyl diphosphate synthase (GGPPS) enzymes in cells and the production of diterpenoids.05-12-2011
20100028945LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING STRESS-RELATED PROTEINS AND USES THEREFOR - Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.02-04-2010
20090123966Hybrid portable origin of replication plasmids - The invention relates to modifying plasmid origins of replication to create hybrid origins of replication containing nucleotide sequences from more than one plasmid. The invention also relates to a modified origin of replication cassette that is portable or exchangeable due to the creation of multiple cloning sites flanking the origin of replication. Methods and plasmids for use in exchanging origins of replication are disclosed. Such modified or hybrid plasmids provide useful cloning tools that allow for regulation of the level of expression of a desired protein.05-14-2009
20100167342METHOD OF PRODUCING BIOLOGICALLY ACTIVE POLYPEPTIDE HAVING INSULINOTROPIC ACTIVITY - The present invention relates to a method of producing a biologically active polypeptide having insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified host cell that has protease gene knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed host cell to produce the biologically active polypeptide; and a method of producing a biologically active polypeptide having an N-terminal recognition site His-Gly with insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified 07-01-2010
20090170157Asparaginases - The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.07-02-2009
20080220473Endo-N-Acetyl-Beta-D-Glucosaminidase Enzymes of Filamentous Fungi - The present invention discloses mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (E.C.3.2.1.96, endo-N-acetyl-beta-D-glucosaminidase acting on the di-N-acetylchitobiosyl part of N-linked glycans) from filamentous fungi such as 09-11-2008
20090275081BIOTIN-LIGASE SYSTEM FOR SECRETION OF BIOTINYLATED PROTEIN - The present invention provides methods of metabolically biotinylating recombinant proteins. Cell lines and specific protein and nucleic acid constructs for use in the methods of the present invention are also provided herein.11-05-2009
20110117598Enhanced Protein Production in Bacillus - The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as 05-19-2011
20090258388METHODS OF ENHANCED HETEROLOGOUS PROTEIN SECRETION - A method of enhancing heterologous protein secretion in a yeast cell is disclosed. In one embodiment, the method comprising the steps of engineering a yeast cell to overexpress at last one gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs, supplying the yeast cell with a nucleic acid encoding a heterologous protein and obtaining increased expression of the heterologous protein, wherein the expression is increased relative to the protein expression in a yeast cell that does not overexpress a gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs.10-15-2009
20100136618METHOD FOR PRODUCING CELLULASE AND HEMICELLULASE HAVING HIGH HYDROLYTIC ACTIVITY - The present invention provides a novel cellulase-producing fungus, i.e. 06-03-2010
20100143970TRANSGLUTAMINASE HAVING DISULFIDE BOND INTRODUCED THEREIN - A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).06-10-2010
20090104657Lipase Production Method - The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus 04-23-2009
20090170159PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION - A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more α-1,2-mannosidases.07-02-2009
20110306090Mammalian Expression Vector - The present invention describes new mammalian expression vectors comprising a novel combination of regulatory elements and one or more selection marker gene(s). The vector allows for incorporation of at least one, preferably two or more genes of interest, its/their subsequent expression, and for selection of transfected cells using, e.g., G418 and/or MTX. The pDGPΔGOI vector as an example for a mammalian expression vector according to the present invention exhibits a 9555 bp sequence, one strand of which is represented by SEQ ID NO:2.12-15-2011
20090280532SERUM-FREE MAMMALIAN CELL CULTURE MEDIUM, AND USES THEREOF - The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.11-12-2009
20090181427HYBRID 3' UNTRANSLATED REGIONS SUITABLE FOR EFFICIENT PROTEIN EXPRESSION IN MAMMALIAN CELLS - The present invention describes the use of hybrid short 3′ untranslated (3′UTR) regions which are composed of two regions, one region from an 3′ untranslated region of a stable eukaryotic mRNA, and another region from the downstream end of an 3′ untranslated region of another eukaryotic mRNA that contains a polyadenylation (polyA) signal. The use of such hybrid regions allows for an efficient protein expression when used in conjunction with circular or linear expression DNA molecules. The present invention provides a recombinant DNA that is composed of a promoter, a protein coding region, and the hybrid 3′UTR in a continuous and directional orientation. The efficient expression systems of the invention are suitable for the economical and efficient production of therapeutic proteins and for use with both transient and stable expression systems.07-16-2009
20100279345AUT0-INDUCIBLE SODIUM PHOSPHATE SYMPORTER PROMOTER FROM PICHIA PASTORIS AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING IT - Disclosed herein are a 11-04-2010
20110306092EXPRESSION VECTOR SYSTEM COMPRISING TWO SELECTION MARKERS - The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least 12-15-2011
20110306091LANTIBIOTIC BIOSYNTHETIC GENE CLUSTERS FROM A. GARBADINENSIS AND A. LIGURIAE - This invention relates to characterisation of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterised biosynthetic gene clusters.12-15-2011
20100068758MUTANT ARABINOSE PROMOTER FOR INDUCIBLE GENE EXPRESSION - An L-arabinose inducible expression system comprising a mutant arabinose promoter. This system exhibits an increase in heterologous protein production upon induction with L-arabinose and comprises a mutant araB promoter and an AraC transcription binding region. This system retains the tight regulatory control characteristic of the wild type arabinose operon.03-18-2010
20110020868BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION - Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.01-27-2011
20090148895Method for Gene Amplification - The present invention provides a double-stranded DNA constructed specifically for high speed gene amplification, a method for gene amplification and a method for synthesizing protein. The gene amplification system of the present invention used a site-specific recombinase such as Cre-lox system and target sequence thereof to efficiently induce a type of replication referred to as a double rolling-circle replication (DRCR). Amplification unit, whose structure is shown in FIG. 06-11-2009
20100297697METHODS FOR INCREASING PROTEIN TITERS - The invention relates to methods of increasing the titre of a protein of interest in a cell as well as the improved production and purification of optimised biomolecules, one component of which is the domain C11-25-2010
20100178671VECTOR CONSTRUCTS AND METHODS FOR EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE - The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5′3′ direction, in functional linkage, 07-15-2010
20090203070HYPERPHOTOSYNTHETIC ORGANISMS - The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.08-13-2009
20090068704UTILIZATION OF STARCH FOR BIOLOGICAL PRODUCTION BY FERMENTATION - This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant 03-12-2009
20110111458INDUSTRIALLY USEFUL MICROORGANISM - An object of the present invention is to provide an 05-12-2011
20100159512METHOD FOR THE PREPARATION OF RECOMBINANT HUMAN THROMBIN AND FIBRINOGEN - The present invention discloses a novel method for the preparation of recombinant human proteins expressed in human cells. Specifically, the present invention relates to novel methods for the preparation of human recombinant thrombin and human recombinant fibrinogen. Moreover, the method employs serum-free culturing conditions and therefore provides recombinant human proteins expressed in human cells of increased safety to the patient when used in human medical treatments. In addition, the immunogenic response to the recombinant human proteins expressed in human cells may be lower. Human recombinant thrombin is expressed in the human embryonic kidney 293 cell line and the protein can be prepared using two different routes, one starting from a point mutated prothrombin with gla and Kringle 1 and 2 domains, and maintaining these domains during the process; the other one starting with prothrombin (non-mutated), via a prethrombin with a HPC4-Kringle 2 domain and subjecting this prethrombin to a point mutation. Human recombinant fibrinogen is expressed in the human embryonic kidney 293 cell line.06-24-2010
20090104656System for the Inducible Expression of Recombinant Proteins in Cyanobacteria - The invention relates to a system for inducible expression in cyanobacteria, which enables the expression of recombinant proteins, and to the vectors and cyanobacteria containing said expression system.04-23-2009
20090142803Amylolytic Enzyme Variants - The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.06-04-2009
20110070611PROCESS FOR PREPARING HUMAN G-CSF - The present invention discloses an improved process for the production of G-CSF in high yield via a high salt-induced increase in plasmid stability during the production phase.03-24-2011
20120064571ZCYTOR19 POLYNUCLEOTIDES, POLYPEPTIDES, ANTIBODIES AND METHODS OF USE - Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor19, a novel class II cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding receptor polypeptides can also be used to block ligand activity in vitro and in vivo. The polynucleotides encoding zcytor19, are located on chromosome 1p36.11, and can be used to identify a region of the genome associated with human disease states. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.03-15-2012
20110039301Polypeptides Having Organophosphorous Hydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.02-17-2011
20120045793EXPRESSION CONSTRUCTS COMPRISING FUNGAL PROMOTERS - The present invention provides promoters derived from a filamentous fungus. These promoters have application in the fields of molecular biology, microbiology, fungal genetics and production of biofuels and other products.02-23-2012
20090061483ACID FUNGAL PROTEASES - The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.03-05-2009
20090298122Translational Elongation Factor Promoter From Pichia Pastoris And Method For Producing Recombinant Protein Using The Same - Disclosed are a 12-03-2009
20110318782Microbial Expression of Tobacco Osmotin for Biocidal and Medical Applications - Disclosed herein are recombinant tobacco osmotin polypeptides and methods for expressing tobacco osmotin polypeptides in microbial host cells. The recombinant tobacco osmotin polypeptides produced by the methods disclosed herein may be utilized as biocides or as therapeutic agents in medicaments.12-29-2011
20090197300MUTUALLY EXCLUSIVE DOMAIN FOLDING MOLECULAR SWITCH AND METHOD OF SYNTHESIS THEREOF - The invention is a fusion protein, embodying a mutually exclusive folding domain molecular switch, wherein the free energy released by folding of a first domain of the fusion protein drives an unfolding of a second domain of the fusion protein, and vice versa. The fusion protein is engineered so that folding the first domain unfolds the second domain, and vice versa, making the folded and unfolded states of the domains mutually exclusive. This is accomplished by insertion of an insert protein GCN4 into a surface loop of a target, protein barnase, subject to die topological design, criterion that the N—C terminal length of GCN4 be at least two-times greater than the Cα-Cα length of a surface loop of barnase. In the absence of the ligand AP-1, barnase is more stable and is folded and active. The presence of AP-1 induces folding of GCN4, forcibly unfolding and inactivating barnase.08-06-2009
20100267087MUTANT PYRROLYSYL-tRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME - Method for incorporating a lysine derivative (particularly an N10-21-2010
20090275078Alpha-Amylase Variants - The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular increased starch affinity relative to the parent alpha-amylase.11-05-2009
20120171721Host Cell Protein Knock-Out Cells for Production of Therapeutic Proteins - The present invention relates to methods and means for making Vitamin K-dependent protein compositions which are devoid or substantially devoid of protein contaminants. In particular, methods and means useful for the reduction or elimination of protein contaminants also being Vitamin K-dependent proteins are described.07-05-2012
20120003694Bioengineered Protein Pores01-05-2012
20080274505Polynucleotide encoding a novel TRP channel family member, LTRPC3, and splice variants thereof - The present invention provides novel polynucleotides encoding LTRPC3 polypeptides, fragments and homologues thereof. The present invention also provides polynucleotides encoding variants and splice variants of LTRPC3 polypeptides, LTRPC3b, LTRPC3c, LTRPC3d, LTRPC3e, and LTRPC3f, respectively. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel LTRPC3, LTRPC3b, LTRPC3c, LTRPC3d, LTRPC3e, and LTRPC3f polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.11-06-2008
20120064570NOVEL CELL PENETRATING PEPTIDE - According to the present invention, it is possible to provide a novel cell penetrating peptide that transports proteins into cells and/or into nuclei at higher frequency than conventional cell penetrating peptides, and a pharmaceutical containing the peptide.03-15-2012
20120115187Production Of Recombinant Proteins Utilizing Non-Antibiotic Selection Methods And The Incorporation Of Non-Natural Amino Acids Therein - Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.05-10-2012
20100216188USE OF CHICK BETA ACTIN GENE INTRON-1 - A method to use chick beta actin gene intron-1 or functional equivalent as a gene expression enhancer element or a gene expression “hot spot” sequence for constructing or reconstructing a mammalian expression vector for extremely high expression of recombinant proteins is disclosed. Composition of a set of extremely strong gene expression vectors is also disclosed.08-26-2010
20090155848NOVEL GLUCOSE DEHYDROGENASE - The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.06-18-2009
20090170156Hydroxynitrile lyase - An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.07-02-2009
20100167341NOVEL PROTEIN EXPRESSION SYSTEM - The present inventors devised a protein expression system with coexisting MiniSeV and SeV particles, and assessed the system for the ability to transfer a gene(s) of interest into target cells, and to express the gene(s) in the target cells. It was shown that the expression system of the present invention had a high ability to transfer a gene(s) of interest into target cells, and a high ability to express the gene(s) in the target cells.07-01-2010
20120208235ENDOGLUCANASE VARIANTS - The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type 08-16-2012
20090075332Modified Proteases - This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as 03-19-2009
20090246828Arabinose Isomerase Expressed from Corynebacterium Genus and Tagatose Manufacturing Method by Using It - The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile 10-01-2009
20110165620METHOD FOR THE PRODUCTION OF PROTEINS OR PROTEIN FRAGMENTS - The present invention relates to a method for selecting a suitable expression construct from a plurality of expression constructs for optimizing the production of a protein or a fragment thereof in a host cell, a method for the production of proteins or fragment thereof using the selected expression vector, to novel human embryonic kidney cells that are deficient in N-acetyl-glucosaminyltransferase I and stably transfected with EBNA (HEK 293E GnTI′cells) that are well suitable for use in the said method, in particular for the production of proteins or protein fragments that are suitable for X-ray studies. The invention also relates to a method to produce HEK 293E GnTI′ cells and a method to confer to HEK293E GnTI′ cells, the capacity to grow in suspension and to a method to confer to HEK293E GnTI′ cells, the capacity to grow in serum free medium. The invention also relates to a kit comprising different vectors suitable for use of the above method for the production of proteins or protein fragments.07-07-2011
20090004696Stabilized bioactive peptides and methods of identification, synthesis, and use - An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.01-01-2009
20110165619Secretion Optimized Microorganism - Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus 07-07-2011
20090053765Variants of Beta-Glucosidase - The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.02-26-2009
20120058512METHOD OF EXPRESSING FOREIGN PROTEIN IN PLASTIDS - The present invention relates to a method of expressing a foreign protein in the plastid of a host cell and secreting said protein into the cytoplasm of the host cell comprising the steps of making a construct of vector linked to a coding sequence of the fusion protein comprising of signal peptide sequence followed by in-frame fusion to a foreign gene; and stably integrating said construct into the plastid genome. The present invention also relates to a method of targeting the expressed and secreted proteins from the plastids to the nucleus of the host cell. The present invention further relates to the method where the host cell is of any higher plant or any organism including single cell algae.03-08-2012
20120058511MUTANT MICROORGANISM AND METHOD FOR PRODUCING PEPTIDE USING THE SAME - The present invention provides a method for producing the peptides comprising: 03-08-2012
20120064569PRODUCTION OF ATTENUATED NEGATIVE STRANDED RNA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES - Attenuated, recombinant negative stranded RNA viruses suitable for vaccine use are produced from one or more isolated polynucleotide molecules encoding the virus. A recombinant genome or antigenome of the subject virus is modified to encode a mutation within a recombinant protein of the virus at one or more amino acid positions(s) corresponding to a site of an attenuating mutation in a heretologous, mutant negative stranded RNA virus. A similar attenuating mutation as identified in the heterologous negative stranded RNA virus is thus incorporated at a corresponding site within the recombinant virus to confer an attenuated phenotype on the recombinant virus. The attenuating mutation incorporated in the recombinant virus may be identical or conservative in relation to the attenuating mutation identified in the heterologous, mutant virus. By the transfer of mutations into recombinant negative stranded RNA viruses in this matter, candidate vaccine viruses are engineered to elicit a desired immune response against a subject virus in a host susceptible to infection thereby.03-15-2012
20080286832Methods for Obtaining Optically Active Epoxides and Vicinal Diols From 2,2-Disubstituted Epoxides - The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific 2,2-disubstituted epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active 2,2-disubstituted vicinal diols and optically active 2,2-disubstituted epoxides.11-20-2008
20090137000DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.05-28-2009
20100227363ALG3 MUTANT - The present invention relates to a method to produce proteins reduced in high mannose type glycans in a plant or plant cell by partially inhibiting the expression of the Alg3 gene in said plant or plant cell. Said partial inhibition can be provided for by inserting the Alg3 mutant gene from 09-09-2010
20120208234PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND - An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.08-16-2012
20120208233ENHANCEMENT OF PROTEIN PRODUCTION IN EUKARYOTIC CELLS - An embodiment relates to the increased expression of polypeptides in eukaryotic cells. A method for increased production is described using an expression vector comprising a polynucleotide encoding a desired polypeptide and a G quartet like motif sequence.08-16-2012
20120107873Method of Producing a Sweet Protein - The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.05-03-2012
20120156722Tumor Suppressor Gene - A full-length cDNA encoding novel proteins involved in the control of cell proliferation (human Gros1-L and S) was successfully isolated from the human testis cDNA libraries. A full-length cDNA encoding the mouse homologues of the human Gros1 (mouse Gros1-L and S) was also isolated. The colony forming activity of cells exogenously expressing Gros1-L was significantly reduced, while that of cells expressing Gros1 antisense RNA was significantly increased.06-21-2012
20120070859BACTERIAL CELLS, OPTIMIZED NUCLEOTIDE SEQUENCES AND METHODS FOR IMPROVED EXPRESSION OF RECOMBINANT CLOSTRIDIUM DIFFICILE TOXIN B - In some embodiments, the present invention provides isolated nucleotide sequences that encode 03-22-2012
20110091935PRODUCTION OF RECOMBINANT PROTEINS IN SYNTHETIC MEDIUM - The invention relates to a method for the production of recombinant proteins by eucaryotic cells in synthetic culture media, wherein said eucaryotic cells are transfected with a composition comprising a synthetic transfection reagent based on a polyhydroxylated polyalkyleneimine.04-21-2011
20110081679MAMMALIAN CELL CULTURE PROCESSES FOR PROTEIN PRODUCTION - The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein.04-07-2011
20100221774Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby - Provided are compositions comprising one or more isoforms of an erythropoietin (“EPO”) comprising glycans linked thereto, wherein the glycans have Lewis x structures and on average at least six sialic acid moieties per EPO molecule. Further provided are methods for obtaining a composition comprising one or more isoforms of EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures, the method comprising: a) providing a eukaryotic cell containing a nucleic acid sequence encoding an adenoviral E1A protein in expressible format and a nucleic acid encoding EPO in expressible format, wherein the cell further contains a nucleic acid sequence encoding a sialyltransferase, e.g., an α-2,6-sialyltransferase or an α-2,3-sialyltransferase, under control of a heterologous promoter; b) culturing the cell in a serum-free culture medium and allowing expression of EPO in the cell; c) harvesting the expressed EPO from the cell and/or from the culture medium; and d) purifying and fractionating the EPO to obtain fractions that have an increased average sialic acid content of the N-linked glycans per EPO molecule, to obtain a composition comprising one or more isoforms of an EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures.09-02-2010
20110065148PEPTIDES AND USES THEREOF - The present invention relates to monovalent fucose-binding peptides. The peptides may be recombinantly produced or chemically synthesized. Preferably the peptides are derived from a lectin, in particular 03-17-2011
20110065147Methods and reagents for modulating cholesterol levels - The invention features ABC1 nucleic acids and polypeptides for the diagnosis and treatment of abnormal cholesterol regulation. The invention also features methods for identifying compounds for modulating cholesterol levels in an animal (e.g., a human).03-17-2011
20110065146Expression of HIV polypeptides and production of virus-like particles - The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides are described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Virus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for the presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.03-17-2011
20090298121EXPRESSION OF SOLUBLE THERAPEUTIC PROTEINS - The present invention provides enhanced methods of producing soluble, active fibroblast growth factor-20 (FGF-20), FGF-21, neurotrophin-3 (NT-3), growth hormone (GH), granulocyte colony stimulating factor (G-CSF), or glucocerebrosidase proteins in microorganisms that have an oxidizing environment.12-03-2009
20120252066METHODS OF FOAM CONTROL - The invention relates to a method for decreasing foam formation as well as maximizing expression of a biosurfactant in a microorganism. The methods encompasses precipitating a biosurfactant from the microorganism which results in decreased form formation.10-04-2012
20110104754CELL CULTURE MEDIUM - We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. Preferably, the cell culture medium comprises a semi-solid medium, which is a serum free or chemically defined medium.05-05-2011
20110104752Variation of Recombinant Expression Titres By Optimising Bacterial Ribosome Binding Sites - The present invention provides a method for optimising the ribosome binding site of a promoter for the expression of a gene encoding a polypeptide of interest, placed under the control of said promoter. The invention also relates to a vector containing such optimised promoters, a prokaryotic host cell transformed by said vector, as well as a method for producing a recombinant protein of interest.05-05-2011
20110104753Soluble Recombinant Influenza Antigens - The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.05-05-2011
20110104750Filamentous Fungi Having Reduced UDP-Galactofuranose Content - A filamentous fungal cell having reduced UDP-galactofuranose is provided. The fungal cell may, in certain embodiments, contain a nuclear genome comprising an inactivated UDP-galactopyranose mutase (UDP-galp mutase) gene and a recombinant nucleic acid for expression of a protein. Also provided are methods of producing a protein using the subject fungal cell, as well as methods of producing the subject fungal cell.05-05-2011
20090263863MODIFICATION OF PROTEIN GLYCOSYLATION IN METHYLOTROPHIC YEAST - The present invention relates to methods and genetically engineered methylotrophic yeast strains for producing glycoproteins with mammalian-like glycosylation. The present invention also relates to vectors useful for generating methylotrophic yeast strains capable of producing glycoproteins with mammalian-like glycosylation. Glycoproteins produced from the genetically engineered methylotrophic yeast strains are also provided.10-22-2009
20100093032Modified Microorganism - To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.04-15-2010
20100093034Alkaline Bacillus Amylase - This invention relates to an amylase derived from 04-15-2010
20100093029MODIFIED LUCIOLA CRUCIATA LUCIFERASE GENE AND PROTEIN - A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from 04-15-2010
20100093026NOVEL ENDORIBONUCLEASE - A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.04-15-2010
20100093027Enzyme - The present invention provides for a substantially pure human or rabbit 3-phosphoinositide-dependent protein kinase.04-15-2010
20120122153ACID-CLEVABLE LINKERS EXHIBITING ALTERED RATES OF ACID HYDROLYSIS - An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.05-17-2012
20120122154METHOD OF PRODUCING LIPIDATED POLYPEPTIDES - A method of producing a recombinant lipidated polypeptide in 05-17-2012
20090130715Antibodies to CD40 - The present invention relates to antibodies and antigen-binding portions thereof that specifically bind to CD40, preferably human CD40, and that function as CD40 agonists. The invention also relates to human anti-CD40 antibodies and antigen-binding portions thereof. The invention also relates to antibodies that are chimeric, bispecific, derivatized, single chain antibodies or portions of fusion proteins. The invention also relates to isolated heavy and light chain immunoglobulins derived from human anti-CD40 antibodies and nucleic acid molecules encoding such immunoglobulins. The present invention also relates to methods of making human anti-CD40 antibodies, compositions comprising these antibodies and methods of using the antibodies and compositions for diagnosis and treatment. The invention also provides gene therapy methods using nucleic acid molecules encoding the heavy and/or light immunoglobulin molecules that comprise the human anti-CD40 antibodies. The invention also relates to transgenic animals comprising nucleic acid molecules of the present invention.05-21-2009
20090130712Compositions and Methods of Producing Hybrid Antigen Binding Molecules and Uses Thereof - This disclosure relates to hybrid antigen binding molecules including at least two polypeptide chains, with at least one polypeptide chain comprises an antigen binding moiety linked to an amino acid sequence of a subunit of a heterodimeric proteinaceous hormone. Also disclosed are methods of making and using such hybrid antigen binding molecules for diagnosis and/or therapy.05-21-2009
20090130713TRICHODERMA PROMOTER - A promoter for use in producing proteins in filamentous fungal host cells is provided. In one embodiment, the promoter comprises SEQ ID NO:1, or a variant or a truncated form thereof that has promoter activity in a host cell. Also provided are recombinant nucleic acids, vectors containing the promoter and host cells containing a recombinant nucleic acid or vector. Methods of producing a protein using the host cells are also provided.05-21-2009
20090130711Recombinant vector capable of increasing secretion of koji mold protease - To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.05-21-2009
20100248307PEPTIDE PRODUCTION METHOD - According to the present invention, a protein having peptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having peptide-synthesizing activity using the transformant and the like, a process for producing a peptide using a protein having peptide-synthesizing activity, and a process for producing a peptide using as an enzyme source a culture or the like of a transformant or a microorganism producing a protein having peptide-synthesizing activity are provided.09-30-2010
20100248304DUAL EXPRESSION VECTOR SYSTEM AND SCREENING METHODS - The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.09-30-2010
20100248305POLYAMINO ACID SYNTHETASE AND GENE ENCODING THE SAME - The present invention provides an enzyme which catalyzes amino acid polymerization in the form of a non-ribosomal peptide synthetase (NRPS) and a gene encoding the same.09-30-2010
20100248308Recombinant Microorganism and a Method for Producing Poly-Gamma-Glutamic Acid - A recombinant microorganism having poly-γ-glutamic acid-producing ability, prepared by introducing a 09-30-2010
20100248306Expression System for the Antibiotic-Free Production of Polypeptides - The invention relates to an expression system for the production of one or more target polypeptide/target polypeptides, comprising a host cell in whose genome the DNA sequence that codes glycerine-3-phosphate dehydrogenase is inactivated or partially or completely deleted and which is transformed by an extrachromosomal element that comprises a DNA sequence that codes the target polypeptide(s) and glycerine-3-phosphate dehydrogenase, whereby not only the host cell genome but also the extrachromosomal element do not carry an antibiotic-resistance gene, as well as a DNA sequence that codes for a polypeptide with glycerine-3-phosphate dehydrogenase activity characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO: 1, c) DNA sequences that are coded by the plasmid pTP01 with the plasmid map according to FIG. 09-30-2010
20110183374THIOPEPTIDE PRECURSOR PROTEIN, GENE ENCODING IT AND USES THEREOF - The present invention relates to the precursor proteins for thiopeptide biosynthesis, and the corresponding structural genes and uses thereof. The present invention also relates to methods for genetically manipulating the thiopeptide precursor protein or host cells expressing a gene encoding said thiopeptide precursor protein to produce thiopeptide compounds or their derivatives. The present invention further relates to the cloning and characterization of genes involved in thiopeptide biosynthesis and their use in thiopeptide compounds production.07-28-2011
20110183375METAL BINDING COMPOUNDS AND THEIR USE IN CELL CULTURE MEDIUM COMPOSITIONS - The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.07-28-2011
20120231500Cells For Transient Expression And Uses Thereof - This invention relates to the transient expression of heterologous polypeptides in mammalian cell lines. Specifically it relates to an expression-enhanced cell line derived from a parent cell line, the expression-enhanced cell line comprising nucleic acid encoding Epstein-Barr Virus Nuclear Antigen 1 or a functional derivative, analogue, or variant thereof; and further comprising: 09-13-2012
20120164686YEAST PROMOTERS - The invention relates to recombinant promoters and expression constructs comprising the promoters that may be used to express a protein of interest in yeast, such as 06-28-2012
20120129217POLYPEPTIDE EXPRESSION IN CILIATES - This invention is directed to methods for recombinant polypeptide production and, in particular, methods and products for the production of recombinant polypeptides in ciliates.05-24-2012
20120231501Methods For Increasing Homologous Recombination Of A Nucleic Acid Sequence - The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.09-13-2012
20100068762NOVEL NUCLEIC ACID MOLECULES - The present invention relates to a novel nucleic acid molecule encoding an amino acid sequence, which is capable of forming a cyclic structure. Cyclization may occur within a cell or cell membrane, or linear forms of the molecules may be circularised or partially circularised, in vitro using isolated enzyme systems or chemical means. The cyclised amino acid sequence is generally in the form of a stabilized folded structure such as acyclic knotted peptide, polypeptide or protein or functional equivalent. The nucleic acid molecules and cyclic and linear peptides are useful inter alia in the generation of molecules having animal or plant therapeutic properties, as well as in a range of diagnostic, industrial and agricultural, including horticultural, applications. Of particular importance is the use of these molecules in the protection of plants, such as crop plants, from pest and/or pathogen infestation.03-18-2010
20120214203Methods for Improving Recombinant Protein Expression - Materials and methods are provided which allowed for increased expression of a transfected gene of interest in a recombinant host cell.08-23-2012
20110177553PREPARATION OF AN ESTERASE - The present invention relates to a recombinant 07-21-2011
20120135463Rhamnose promoter expression system - Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: 05-31-2012
20120135464STIRRER SYSTEM - The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.05-31-2012
20120214202RECOMBINANT HOST CELLS HAVING AN INCREASE IN BUOYANT DENSITY - Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.08-23-2012
20120252068Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.10-04-2012
20100196960TRANSPORTER GENES OATP-B, C, D, AND E - Four novel transporter genes were successfully cloned by screening novel transporter genes based on the human OATP transporter gene sequence. These transporters are useful in the development of drugs by taking advantage of the activity of transporting biological substances and various drugs. It was also found that these transporter genes have single nucleotide polymorphisms (SNP). Gene diagnosis based on the polymorphisms (such as SNP) in these transporter genes enables one to judge, for example, the efficacy of a drug therapy.08-05-2010
20120077227Expression Process - An expression system for the production of a target polypeptide is provided. The expression system comprises an expression cassette comprising an inducible promoter operably linked to DNA encoding the target polypeptide and an expression cassette for over-expression of DNA binding transcriptional regulator protein, comprising a promoter operably linked to DNA encoding the DNA binding transcriptional regulator protein. The expression cassettes are under the control of orthogonal promoters.03-29-2012
20120077226SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS - A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.03-29-2012
20120077225Melibiose operon expression system - New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.03-29-2012
20090075334POLYPEPTIDES, THEIR PRODUCTION AND USE - This invention relates to a novel polypeptide involving in the modulation of central nervous system function, circulatory function, immune function, gastrointestinal function, metabolic function, reproductive function, etc., it can be used as a drug for treating or preventing a variety of diseases, e.g. HIV infection or AIDS (acquired immune deficiency syndrome) or the like.03-19-2009
20100047866Heat-Stable Carbonic Anhydrases and Their Use - The present invention relates to use of heat-stable carbonic anhydrase in CO02-25-2010
20100047869Novel Thermophilic Endo-Glucanase and Uses Thereof - A novel thermophilic endo-glucanase, nucleic acid encoding the endo-glucase, and uses thereof in converting lignocellulosic material to fermentable sugars.02-25-2010
20100047868Tumour Suppressor Protein - We describe a polypeptide which binds and modulates the activity of a tumour suppressor polypeptide, for example p53; a nucleic acid molecule encoding said protein and screening methods which modulate the binding activity of said polypeptide for its target polypeptide(s).02-25-2010
20100047867IL-1 ZETA, IL-1 ZETA SPLICE VARIANTS AND XREC2 DNAS AND POLYPEPTIDES - The invention is directed to novel, purified and isolated IL-1 zeta and Xrec2 polypeptides and fragments thereof, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and uses thereof.02-25-2010
20100047864PROCESS FOR THE PRODUCTION OF RECOMBINANT PROTEINS USING CARNIVOROUS PLANTS - The present application relates to the provision of process for producing at least one protein, comprising the cultivation of a carnivorous plant, characterized in that said plant has been genetically modified to express said protein or proteins, and said protein or proteins are collected from the digestive secretions of said carnivorous plant traps, in particular glue, pitcher, trumpet or bladder traps. The proteins of interest are functional, despite the existence of digestive enzymes.02-25-2010
20100047863GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY - The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites.02-25-2010
20100047862Novel DNA Polymerase - This invention provides a novel DNA polymerase obtained from 02-25-2010
20100047861PROCESS FOR PRODUCING USEFUL SUBSTANCE - The present invention provides processes of producing useful substances, which include the steps of culturing in a medium microorganisms that lack from their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein that is 80% or more homologous to the amino acid sequence shown in SEQ ID NO: 1 so as to produce and accumulate the useful substances in the culture, and recovering the useful substances from the culture.02-25-2010
20120219989Polypeptides Having Glucoamylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.08-30-2012
20120178125Mutant Strain of Aspergillus sojae with Enhanced Protease Activity and Preparation Method of Natural Taste Enhancer Using the Same - The present invention relates to a mutant strain of 07-12-2012
20100015663METHOD FOR PRODUCING AN ANTIFUNGAL PEPTIDE IN A FILAMENTOUS FUNGAL HOST CELL - The present invention provides a method for producing an antifungal peptide in a filamentous fungal host cell by expressing the antifungal peptide in a host cell which is deficient or partially deficient in the expression of an endogenous glucosyl-ceramide synthase (gcs) gene.01-21-2010
20100273215 Secretion Yield of a Protein of Interest by in vivo Proteolytic Processing of a Multimeric Precursor - The invention relates to a nucleic acid molecule encoding a multimeric precursor which after transcription is specifically cleaved in vivo to form multiple copies of a protein of interest. The invention further relates to a cell comprising this nucleic acid molecule and a method for producing a protein of interest using this cell.10-28-2010
20100273212PP1 Ligands - The invention relates to phosphatase ligands and polyligands. In particular, the invention relates to ligands, homopolyligands, and heteropolyligands that modulate PP1 activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands, homopolyligands, and heteropolyligands to a cellular localization signal, epitope tag and/or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.10-28-2010
20100273211Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy - Botulinum neurotoxins, the most potent of all toxins, induce lethal neuromuscular paralysis by inhibiting exocytosis at the neuromuscular junction. The light chains (LC) of these dichain neurotoxins are a new class of zinc-endopeptidases that specifically cleave the synaptosomal proteins, SNAP-25, VAMP, or syntaxin at discrete sites. The present invention relates to the construction, expression, purification, and use of synthetic or recombinant botulinum neurotoxin genes. For example, a synthetic gene for the LC of the botulinum neurotoxin serotype A (BoNT/A) was constructed and overexpressed in 10-28-2010
20100009407Processing Enzymes Fused to Basic Protein Tags - The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.01-14-2010
20100285527Novel Polypeptide, cDNA Encoding the Same, and Use Thereof - Novel polypeptides produced by a human adult brain tissue, a cell line derived therefrom, a cell line derived from human bone marrow and a human umbilical cord venous endothelial cell line; a process for producing these polypeptides; cDNAs encoding the polypeptides; fragments hybridizable selectively with the cDNA sequences; replication or expression plasmids having the cDNAs integrated thereinto; host cells transformed by the plasmids; antibodies against the above polypeptides; and medicinal compositions containing the peptides or the antibodies.11-11-2010
20090061484ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC FEEDSTOCKS USING ACCESSORY ENZYMES - Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (f03-05-2009
20090061480NUCLEOTIDE SEQUENCE ENCODING A MODULATOR OF NF-KB - The present invention relates to nucleotide sequences encoding a modulator of NF-κB, and to the polypeptides encoded by the nucleotide sequences. In particular, the invention relates to nucleotide sequences and the polypeptides encoded thereby, wherein the polypeptides are involved in the response to NF-κB-activating stimuli, including HTLV-1 Tax, LPS, PMA and IL-1. The invention also relates to antibodies to the modulator of NF-κB, methods of detecting modulator of NF-κB using the antibodies, methods of treatment associated with NF-κB activation and to methods of identifying compounds which modulate the activity of the modulator of NF-κB.03-05-2009
20100003719Trimerising Module - The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.01-07-2010
20120315670Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell - Methods and compositions are provided for manipulating the genome of host cell to produce at least one exogenous gene product. Also provided are methods and composition for producing a programmable cell comprising a plurality of exogenous genes, wherein each exogenous gene is under the control of a disrupted regulatory sequence and wherein the disrupted regulatory sequences are restored by in vivo recombination. Preferably, the gene of interest is under the control of a genetically altered promoter which sequence recombination effects the expression of the exogenous gene(s).12-13-2012
20100291626ENHANCED EXPRESSION AND STABILITY REGIONS - Expression-enhancing nucleotide sequences for expression in eukaryotic systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Enhanced expression and stability regions (EESYRs) are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells.11-18-2010
20100291625REGULATION OF TRANSLATION OF HETEROLOGOUSLY EXPRESSED GENES - The present invention pertains to a method of expressing a protein of interest, preferably a heterologous protein, in preferably a plant. In a preferred embodiment said plant is a doubled haploid homozygous transgenic 11-18-2010
20100291624COMPOSITIONS AND METHODS FOR IMPROVED GLYCOPROTEIN SIALYLATION - The present invention provides compositions and methods for the production of glycoproteins with enhanced sialylation. In particular, the invention provides cell lines comprising disrupted sialidase expression and methods of using the cell lines to produce glycoproteins with enhanced sialylation.11-18-2010
20120225453SYSTEMS AND METHODS FOR THE SECRETION OF RECOMBINANT PROTEINS IN GRAM NEGATIVE BACTERIA - Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant 09-06-2012
20120258493PROMOTER SEQUENCES - The present invention relates to new promoter sequences and uses thereof, in particular expression cassettes, vectors, and methods of expressing genes using the new promoters.10-11-2012
20120258492BACTERIAL HOST STRAIN - A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.10-11-2012
20120083014Nicotiana Benthamiana Plants Deficient in Xylosyltransferase Activity - The invention provides 04-05-2012
20120258491EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED - Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.10-11-2012
20120190065COMBINATORIAL ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through introduction of nucleic acids encoding UBF or reducing expression of NoRC proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method.07-26-2012
20090035821Expression of Proteins in E.Coli - Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX02-05-2009
20120264169DNASE EXPRESSION IN RECOMBINANT HOST CELLS - The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: 10-18-2012
20120264168ZCYTOR17 HETERODIMERIC CYTOKINE RECEPTOR POLYNUCLEOTIDES - Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor17-containing multimeric or heterodimer cytokine receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. The present invention also includes methods for producing the multimeric or heterodimeric cytokine receptor, uses therefor and antibodies thereto.10-18-2012
20090017493Gene SMS 02 - The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.01-15-2009
20090017492SVPH1-26 DNA and polypeptides - DNA encoding SVPH1-26 polypeptides and methods for using the encoded proteinase and polypeptides are disclosed. SVPH1-26 is expressed in testis.01-15-2009
20090004694Sequence of Thermotolerant L-Rhamnose Isomerase Gene and Use of the Same - [PROBLEMS] To provide the sequence of a thermotolerant L-rhamnose isomerase gene. [MEANS FOR SOLVING PROBLEMS] A DNA comprising the base sequence represented by SEQ ID NO:1. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A protein originating in 01-01-2009
20110124047VMP-LIKE SEQUENCES OF PATHOGENIC BORRELIA SPECIES AND STRAINS - The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic 05-26-2011
20110124046Extracellular Secretion of Recombinant Proteins - Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocelluiosic biomass.05-26-2011
20110124045NUCLEIC ACIDS ENCODING RECOMBINANT PROTEIN A - Disclosed are new recombinant nucleic acids encoding protein A polypeptides and methods of using these nucleic acids.05-26-2011
20100297698SYSTEM AND METHOD FOR PRODUCING SYNTHETIC MICROORGANISMS CAPABLE OF TRANSLATING PROTEINS CONTAINING NON-STANDARD AMINO ACIDS - The disclosed invention relates to the generation of host cells containing rare codons and/or absent tRNAs, and the use of orthogonal tRNA systems that can insert a non-standard amino acid into a growing peptide chain. This invention combined with the capacity to synthesize whole genomes has important implications in synthetic biology, as it allows the rewriting of the genetic code of existing or newly designed organisms.11-25-2010
20120231502METHOD FOR PRODUCING THERAPEUTIC PROTEINS IN PICHIA PASTORIS LACKING DIPEPTIDYL AMINOPEPTIDASE ACTIVITY - The present invention related to methods and compositions for producing therapeutic proteins in yeast cell lines, and in particular 09-13-2012
20120231499HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM - A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.09-13-2012
20090280528Method for Cloning and Expression of NruI Restriction Endonuclease - Recombinant DNA encoding NruI- and SboI-like restriction endonucleases and methylases and their amino acid sequences are provided as well as methods for expressing the enzymes in transformed host cells and purifying the enzymes.11-12-2009
20080299609Uracil-DNA glycosylase of psychrobacter sp. HJ147 and use thereof - The present invention provides uracil-DNA glycosylase (UDG) gene originating from 12-04-2008
20080299613Compositions for degrading cellulosic material - The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.12-04-2008
20080299615Beta1,3-N-acetyl-D-galactosamine transferase protein, nucleic acid encoding the same and method of examining canceration using the same - The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes under stringent conditions to the nucleotide sequence shown in SEQ ID NO: 1 or 3 or a nucleotide sequence complementary to at least one of them.12-04-2008
20080299611Host Cell Protein Knock-Out Cells for Production of Therapeutic Proteins - The present invention relates to methods and means for making Vitamin K-dependent protein compositions which are devoid or substantially devoid of protein contaminants. In particular, methods and means useful for the reduction or elimination of protein contaminants also being Vitamin K-dependent proteins are described.12-04-2008
20080299612FUSION PROTEINS WITH CLEAVABLE SPACERS AND USES THEREOF - A polypeptide comprising a first protein domain, a second protein domain, and a dithiocyclopeptide spacer containing at least one protease cleavage site, wherein the dithiocyclopeptide is exogenous relative to the first or second protein domain, and wherein the first and second protein domains are operably linked by the dithiocyclopeptide. Also disclosed are methods of producing the polypeptide and delivering the protein domains into a cell.12-04-2008
20080299610Human toll homologue - The invention relates to the identification and isolation of novel DNAs encoding the human Toll protein PRO358 and its variants, and to methods and means for the recombinant production of these proteins. The invention also concerns antibodies specifically binding the PRO358.12-04-2008
20080299614Metabolically Engineered Bacterial Strains Having Non-Functional Endogenous Gluconate Transporters - The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.12-04-2008
20120322104SYSTEMS AND METHODS FOR PROTEIN PRODUCTION - The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.12-20-2012
20120237974METHOD FOR THE EXPRESSION OF A RECOMBINANT PROTEIN IN A MAMMALIAN CELL - The invention relates to methods for the production of a recombinant protein in a mammalian cell and methods to enhance the production of recombinant proteins in mammalian cells. More in particular, the invention provides a cell for the production of a recombinant protein of interest wherein said cell is permissive to a polyomavirus and wherein said cell comprises the genetic elements A and B wherein A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of a polyomaviral origin of replication or a functional equivalent thereof, wherein said cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein.09-20-2012
20080293098Dna Encoding Novel Enzyme Having D-Serine Synthase Activity, Method of Producing the Enzyme and Method of Producing D-Serine by Using the Same - This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.11-27-2008
20120322105GROWTH FACTOR - VEGF-D, new member of the PDGF family of growth factors, which among other things stimulates endothelial cell proliferation and angiogenesis and increases vascular permeability, as well as nucleotide sequences encoding it, methods for producing it, antibodies and other antagonists to it, transfected or transformed host cells for expressing it, pharmaceutical compositions containing it, and uses thereof in medical and diagnostic applications.12-20-2012
20120322106Production of Cellulases and Hemicellulases in Algal Biofuel Feedstocks - A method for producing hemicellulase and cellulase for use in processing cellulosic materials for biofuel production. The method involves transforming algal feedstock to express hemicellulase and cellulase and then collecting hemicellulase and cellulase that has been secreted into a culture medium by the algae. The method may further involve transforming the algal feedstock to express other recombinant products and/or processing the alga feedstock to produce biofuels.12-20-2012
20120322102GENETICALLY ENGINEERED HERBICIDE RESISTANT ALGAE - Algae transformed with one or more polynucleotides encoding proteins that confer herbicide resistance are provided. The algae can be grown in small or large scale cultures that include one or more herbicides for the production and isolation of various products.12-20-2012
20120322103Scalable Fermentation Process - This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.12-20-2012
20090081731Method of producing catalytically active BACE2 enzymes - The invention provides methods and compositions for the efficient expression and isolation of BACE2 polypeptides from inclusion bodies and its refolding to an active enzyme.03-26-2009
20100190209OPTIMIZED DNA SEQUENCES ENCODING RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 (RHBMP-2), PREPARATION METHOD AND THE USES THEREOF - The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the 07-29-2010
20110045533PRODUCTION OF GLYCOSYLATED POLYPEPTIDES IN MICRO ALGAE - Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.02-24-2011
20110045531METHOD FOR PRODUCING A RECOMBINANT PROTEIN ON A MANUFACTURING SCALE - A method for producing a protein of interest on a manufacturing scale is based on integration, by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell genome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.02-24-2011
20090068703PROTEIN EXPRESSION SYSTEMS - Disclosed are recombinant non-insect host cells and use of activation of CMV or SV40 promoters by IE1, IE2 and the enhancer hr of the baculovirus in non-insect host cells for expression heterologous RNAs or polypeptides. Also disclosed are methods of using the cells.03-12-2009
20100203588POLYPEPTIDES FOR INDUCING A PROTECTIVE IMMUNE RESPONSE AGAINST STAPHYLOCOCCUS AUREUS - The present invention features polypeptides comprising an amino acid sequence structurally related to SEQ ID NO: 1 or a fragment thereof, 08-12-2010
20100203586MODIFIED 13-HYDROPEROXIDE LYASES AND USES THEREOF - Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.08-12-2010
20100203585Calcium-Binding Photoprotein, Gene Encoding the Same, and Use Thereof - A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.08-12-2010
20100203584Host Cells Used For Production of Recombinant Protein - It is an object of the present invention to provide host cells, which are able to proliferate in a serum free medium, which are able to synthesize and secrete a large amount of protein of interest, and which are able to produce a glycoprotein having a sugar chain structure. The present invention provides an established cell line, which is established from distal tubular epithelial cells derived from a mammal.08-12-2010
20110236928Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum - The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent 09-29-2011
20110236927GENETICALLY STABILIZED TANDEM GENE DUPLICATION - The invention provides constructs and methods for producing genetically stabilized tandem gene duplications.09-29-2011
20100196956NOVEL CONSTITUTIVE STRONG PROMOTER AND USE THEREOF - Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from 08-05-2010
20100233759METHOD FOR PRODUCTION OF POLYPEPTIDE - The present invention provides a method capable of producing a natural or recombinant protein in high yield.09-16-2010
20100233758Protein increasing cell infectivity of herpes simplex virus and use thereof - Provided are an isolated protein derived from an N-terminal of HveA/HVEM and having activity of increasing the cell infectivity of herpes simplex virus (HSV) and use thereof.09-16-2010
20100233760Protein Production in Microorganisms of the Phylum Labyrinthulomycota - The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.09-16-2010
20120276588Use Of Galerina Marginata Genes And Proteins For Peptide Production - The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from 11-01-2012
20120276589METHOD FOR PRODUCING PROTEINS COMPRISING NON-NATURAL AMINO ACIDS INCORPORATED THEREIN - Producing proteins incorporating non-natural amino acids can involve introducing genes into and knocking inherent genes out of eukaryote-type cells. Genes to be introduced include genes encoding eukaryote-type aminoacyl tRNA synthetase mutants having enhanced specificity to non-natural amino acids, compared with specificity to similar natural amino acids, and tRNA genes for non-natural amino acids capable of binding to the non-natural amino acids in the presence of the eukaryote-type aminoacyl tRNA synthetase mutants. Inherent genes to be knocked out include genes encoding aminoacyl tRNA synthetase having specificity to natural amino acids and tRNA genes capable of binding to the natural amino acids in the presence of the inherent aminoacyl tRNA synthetase.11-01-2012
20120276590RECOMBINANT SILKWORM AND SILKWORM PROTEIN COMPRISING HETEROLOGOUS PROTEIN PRODUCED BY THE RECOMBINANT SILKWORM - The present invention relates to a recombinant organism having any one of nucleic acids (i) to (iv) introduced therein: (i) a nucleic acid having a base sequence of SEQ ID NO: 1; (ii) a nucleic acid encoding a protein having an amino acid sequence of SEQ ID NO: 2; (iii) a nucleic acid encoding a dragline protein and having a sequence identity of 90% or more with the nucleic acid (i); (iv) a nucleic acid which encodes a dragline protein and hybridizes with a complementary chain of the nucleic acid (i) under stringent conditions.11-01-2012
20110262964Reagentless fluorescent biosensors comprising a designed ankyrin repeat protein module, rational design methods to create reagentless fluorescent biosensors and methods of their use - The present invention relates to reagentless fluorescent biosensors which comprise at least one ankyrin repeat and a fluorophore and are specific for at least one target; the method for preparing such reagentless fluorescent biosensors comprises the following steps: (a) identifying the residues (R10-27-2011
20120329090Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells - The present invention relates to methods of producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent bacterial cell in a medium for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a first polynucleotide encoding the heterologous polypeptide which comprises two or more (several) cysteines, and a second polynucleotide comprising a modification of a gene encoding a thiol-disulfide oxidoreductase that incorrectly catalyzes the formation of one or more (several) disulfide bonds between the two or more (several) cysteines of the heterologous polypeptide, and (ii) the mutant cell is deficient in production of the thiol-disulfide oxidoreductase compared to the parent bacterial cell when cultivated under the same conditions; and (b) recovering the heterologous polypeptide from the cultivation medium. The present invention also relates to such bacterial mutants and methods for producing such bacterial mutants.12-27-2012
20120329091RECOMBINANT PROTEIN PRODUCTION IN HETEROLOGOUS SYSTEMS - Isolated polynucleotides are disclosed which increase the efficiency of gene expression in a heterologous cell. The polynucleotide sequences which encode polypeptides are adapted such that the average rate of translation of the first at least about 30 amino acids is slower by at least two fold than the average rate of translation of the remaining amino acids of the polypeptide.12-27-2012
20120100574FLAVIVIRUS-BASED SYSTEM FOR PRODUCTION OF HEPATITIS C VIRUS (HCV) - Provided herein is a mammalian cell transformed to contain a plasmid encoding a T7 or SP6 promoter operably linked to one or more HCV genes, a subgenomic replicon from a flavivirus and a cytoplasmic T7 and SP6 RNA amplification system. Also provided herein are isolated replication-competent HCV particles produced by the method comprising the steps of providing a transformed mammalian cell according to the first embodiment, culturing the cell, and recovering the replication-competent HCV particles from the cell culture. Provided herein are isolated HCV structural proteins produced by the method comprising the steps of providing a transformed mammalian cell according to the first embodiment, culturing the cell, and recovering the HCV structural proteins from the cell culture. Further provided herein is a system for assaying HCV entry into a cell comprising a first plasmid encoding a T7 or SP6 promoter operably linked to an HCV polynucleotide comprising at least the 5′-UTR to NS2 operably linked to an EMCV IRES in frame with an SP6 or T7 polymerase gene, respectively, a first host cell line expressing a replicon from a flavivirus and comprising a cytoplasmic T7 and SP6 RNA amplification system, a second plasmid encoding a reporter gene operably linked to both T7 and SP6 promoters in tandem, and a second host cell line comprising a cytoplasmic T7 polymerase or SP6 polymerase RNA amplification system.04-26-2012
20120100573CYTOMEGALOVIRUS INTRON A FRAGMENTS - Cytomegalovirus (CMV) Intron A fragments for expressing gene products are disclosed. Also described are expression vectors including the fragments, as well as methods of using the same.04-26-2012
20100227362 Novel YLMPO1 Gene Derived From Yarrowia Lipolytica and A Process for Preparing a Glycoprotein Not Being Mannosylphosphorylated By Using a Mutated Yarrowia Lipolytica in Which YLMPO1 Gene is Disrupted - The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast 09-09-2010
20100209969GERANIOL SYNTHASE, METHODS OF PRODUCTION AND USES THEREOF - The present invention relates to novel type of monoterpene synthase, a key enzyme in the production of the monoterpene aroma compound geraniol. More specifically, the present invention relates to nucleic acid sequences coding for GES from flowers and herbs species, in particular sweet basil, as well as to vectors containing the sequences, to host cells containing the sequences and to methods of producing recombinant GES, its products, and uses thereof.08-19-2010
20100028941Method for Multiparallel Construction of Host/Vector-Systems for Expression of Proteins - The present invention relates to a multiparallel method for construction of host/vector-systems, comprising transforming a plurality of host strains, wherein substantially all host strains have deleterious mutations in at least one uptake system for a critical substrate needed for the growth of said host strain, with a plurality of vectors encoding at least one protein, and expressing said recombinant protein in said host strains. It further relates to host/vector systems obtainable by the method, to kits comprising the host strains and optionally the vectors, and to use of produced host/vector systems for protein expression. The method is exemplified using an 02-04-2010
20100136619Expression System - A protein expression system is provided. The system comprises: a) a T7 RNA polymerase-dependent promoter operably linked to an expression cassette for a protein of interest; and b) an expression cassette for T7 RNA polymerase operably linked to a λpL promoter and at least two perfect palindrome operator sequences.06-03-2010
20100167344NOVEL SIALIDASES - The present invention relates to an isolated polypeptide which has sialidase activity, selected from the group consisting of: (a) a polypeptide which has an amino acid sequence which has at least 60% amino acid sequence identity with amino acids 1 to 407 of SEQ ID NO: 3; (b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1 or 2 which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO: 1 or 2.07-01-2010
20100167343OVIDUCT SPECIFIC EXPRESSION PROMOTER AND RECOMBINANT EXPRESSION VECTOR COMPRISING THE SAME - An oviduct specific expression promoter and a recombinant expression vector including the same. A promoter of an AGR2 gene is expressed specifically in the chicken oviduct, and a recombinant expression vector includes the promoter and a desired gene for encoding a desired protein. The oviduct specific promoter and the recombinant expression vector including the promoter can induce the expression of a protein specifically in the oviduct, i.e., an organ that secrets proteins so as to accumulate with a large amount in the egg. Accordingly, the oviduct specific promoter and the recombinant expression vector of the present invention can be advantageously used to produce transformed chickens, which can massively yield useful elements of high added value, produce functional eggs, and improve the economic traits of the poultry.07-01-2010
20100167345HALOHYDRIN DEHALOGENASES AND RELATED POLYNUCLEOTIDES - The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.07-01-2010
20130011874REDUCED GENOME E. COLI - Reduced genome strains of 01-10-2013
20130011875METHODS FOR THE PRODUCTION OF RECOMBINANT PROTEINS WITH IMPROVED SECRETION EFFICIENCIES - The present invention is related to methods and for producing higher titers of recombinant protein in a modified yeast host cell, for example 01-10-2013
20130011876PROCESS FOR PREPARING FILAMENTOUS FUNGAL STRAINS HAVING A SEXUAL CYCLE AND A PROCESS FOR PREPARING SEXUALLY CROSSED FILAMENTOUS FUNGAL STRAINS - The invention relates to a process for preparing filamentous fungal strains having a sexual cycle, wherein an acceptor filamentous fungal strain, having no or one type MAT locus, is subjected to recombination in which one or more mating type locus genes and optionally sex related genes, from other species than the acceptor filamentous fungal strain, is introduced into the acceptor filamentous fungal strain to produce a filamentous fungal strain having a sexual cycle.01-10-2013
20100129868DELTA 4,5 GLYCURONIDASE COMPOSITIONS AND METHODS RELATED THERETO - The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof.05-27-2010
20100129863Optimized core promoters and uses thereof - The present invention provides core promoter motif ten elements (MTE) and core promoter constructs comprising the MTEs and an initiator element (Inr) in combination with one or both of a TATA box and a downstream promoter element (DPE) which increases gene expression over the strongest known core promoters. Particularly, an optimized or super core promoter is provided which comprises Inr, MTE, TATA box and DPE elements. The present invention also provides expression vectors and host cells comprising the core promoter constructs. Additionally, methods of increasing production of a protein using the core promoter constructs are provided.05-27-2010
20100129864Novel Bacteriocins, Transport and Vector System and Method of Use Thereof - New bacteriocins capable of inhibiting the growth of bacteria are disclosed, along with methods of obtaining secretion of proteins from lactic acid bacteria, and methods for protecting foodstuffs.05-27-2010
20100129866METHOD FOR PRODUCTION OF DIPEPTIDE - According to the present invention, a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant or the like, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source are provided.05-27-2010
20100129862Novel lipolytic Enzyme lip1 - The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.05-27-2010
20080254506Claudin polypeptides - This invention relates to new members of the human Claudin polypeptide family, to methods of making such polypeptides, and to methods of using them to treat Claudin-associated conditions and to identify compounds that alter Claudin polypeptide activities.10-16-2008
20130171692SUGAR-CHAIN MODIFIED YEAST AND METHOD FOR PRODUCING GLYCOPROTEIN USING THE SAME - The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man07-04-2013
20080248527Binding Phenol Oxidizing Enzyme-Peptide Complexes - The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically 10-09-2008
20080248526Recombinant Cell Lines for the Stable and High-Level Production of Biologically Active Dkk1 Protein - This invention relates to the use of specific recombinant cell lines in order to produce biologically active DKK1 protein in a stable and high-level manner. The invention also relates to these recombinant cell lines, as well as a method for stable and high-level production of biologically active DKK1 protein, implementing such recombinant cell lines.10-09-2008
20080241883Recombinant expression vector elements (rEVEs) for enhancing expression of recombinant proteins in host cells - Compositions and methods comprising recombinant expression vector elements (rEVEs) to enhance the level of expression of recombinant proteins are described. Other compositions and methods for lowering, substantially suppressing, or essentially silencing expression of a recombinant protein are also described.10-02-2008
20080241882Methods and materials relating to novel stem cell growth factor-like polypeptides and polynucleotides - The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human stem cell growth factor-like protein. These polynucleotides comprise nucleic acid sequences isolated from cDNA libraries from human testis cells (Hyseq clone identification numbers 2880984 and 2881695), from human fetal skin (Hyseq clone identification number 15375176), adult spleen (Hyseq clone identification number 14856094), and human endothelial cells (Hyseq clone identification numbers 13804756, 13687487, 13804756). Other aspects of the invention include vectors containing processes fro producing novel human stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.10-02-2008
20080241881MODIFIED CLOSTRIDIAL TOXINS WITH ENHANCED TRANSLOCATION CAPABILITIES AND ALTERED TARGETING ACTIVITY FOR CLOSTRIDIAL TOXIN TARGET CELLS - The specification discloses modified Clostridial toxins comprising a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain, a translocation facilitating domain and an altered target domain; polynucleotide molecules encoding such modified Clostridial toxins; and methods of producing such modified Clostridial toxins.10-02-2008
20080241880Methods and compositions for determination of glycated proteins - This invention relates generally to the field of glycated protein detection. In particular, the invention provides chimeric proteins, nucleic acids encoding the chimeric proteins, methods and kits for assaying for a glycated protein in a sample, using inter alia, an amadoriase.10-02-2008
20080233614PRODUCTION OF RECOMBINANT COLLAGENASES COLG AND COLH IN ESCHERICHIA COLI - The present invention provides codon-optimized genes designed to help maximize heterologous protein expression level. The present invention provides codon-optimized recombinant colG and colH collagenase sequences.09-25-2008
20080233613Polypeptides having beta-glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.09-25-2008
20080233612Fungal Hosts for Expression of Recombinant Products - An isolated mutant 09-25-2008
20080227156NUCLEIC ACIDS ENCODING NEUTRALIZING GDF8 EPITOPE-BASED PEPTIDES AND FUSION PROTEINS - The invention provides new, specific antigenic peptides from the protein GDF8. The invention also provides fusion proteins comprising the new peptides, immunogens and vaccines based on the new peptides and/or fusion proteins, antibodies that specifically bind to the new peptides of GDF8, and methods of treating animals in order to modulate the activity of GDF8, employing vaccines or antibodies according to the invention.09-18-2008
20080227155PEGYLATED SOLUBLE GP130-DIMERS USEFUL AS A MEDICAMENT - A polypeptide-dimer comprising two soluble gp130 molecules is described, wherein at least one of said soluble gp130 molecules is covalently linked to polyethylene glycol. Furthermore, a pharmaceutical composition containing said dimer and various medical uses are described.09-18-2008
20080227154Protein Expression - Disclosed is chimeric polynucleotides adopted for providing high-yield expression and secretion of recombinant polypeptides. The chimeric polynucleotide of the invention includes a functional secretion signal peptide derived from 09-18-2008
20080227153Site specific incorporation of keto amino acids into proteins - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.09-18-2008
20080227152In vivo incorporation of unnatural amino acids - The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.09-18-2008
20080227151Method for simultaneous production of multiple proteins; vectors and cells for use therein - Described is the production of proteins in a host cell. Even more specifically, described are methods for improving expression of two or more proteins in a cells or host cell. The methods are suited for production of, for example, recombinant antibodies that can be used in pharmaceutical preparations or as diagnostic tools. In certain embodiments, provided are methods for obtaining a cell that expresses two or more proteins comprising providing the cell with two or more protein expression units encoding two or more proteins, characterized in that at least two of the protein expression units comprise at least one STAR sequence.09-18-2008
20080227150OVEREXPRESSION OF PHYTASE GENES IN YEAST SYSTEMS - The present invention relates to a method of producing a heterologous protein or polypeptide having phytase activity in a yeast system. The invention also provides proteins having phytase activity which have increased thermostability. Yeast strains which produce a heterologous phytase and the vectors used to produce the phytase are also provided.09-18-2008
20080227149Interferon-Alpha Polypeptides and Conjugates - The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.09-18-2008
20080227148Aspergillus Promotors for Expressing a Gene in a Fungal Cell - The present invention relates to isolated promoter DNA sequences, to DNA constructs, vectors, and host cells comprising these promoters in operative association with coding sequences. The present invention also relates to methods for expressing a gene and/or producing a biological compound using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.09-18-2008
20080227147Fungal Cell Wall Degrading Enzyme - A novel fungal enzyme having lysozyme activity has been isolated. The invention further relates to a fungal polypeptide having lysozyme activity and belonging to the GH25 family, wherein the enzyme is selected from the group consisting of (a) a polypeptide comprising an amino acid sequence, which has at least 80% identity with amino acids 1 to 233 of SEQ ID NO:2; (b) a polypeptide comprising an amino acid sequence, which has at least 80% identity with the polypeptide encoded by the lysozyme encoding part of the nucleotide sequence inserted into a plasmid present in strain DSM 16084; (c) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe consisting of the complementary strand of nucleotides 84 to 782 of SEQ ID NO:1; or (d) a fragment of (a), (b) or (c) that has lysozyme activity.09-18-2008
20080227146Immortalized Avian Cell Lines For Virus Production - The present invention relates to immortalized avian cell lines suitable for production of biologicals or viruses for vaccination. In particular, the cell lines are derived from primary cells which are transformed with at least two viral or cellular genes, one of which causes cell cycle progression whereas the other interferes with innate protective mechanisms of the cell induced by dysregulated replication. The invention moreover relates to the production of said immortalized cell lines and their use for producing biologicals or viruses for vaccination.09-18-2008
20110262965CELL CULTURE MEDIUM COMPRISING SMALL PEPTIDES - Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.10-27-2011
20080220477NON-NATURAL LABELED AMINO ACID AND METHOD FOR PRODUCING A CONJUGATE OF SAID AMINO ACID AND TRNA - This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.09-11-2008
20080220476Thermostable Alpha-Amylases - An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: 09-11-2008
20080220475mTOR LIGANDS AND POLYNUCLEOTIDES ENCODING mTOR LIGANDS - The invention relates to kinase ligands and polyligands. In particular, the invention relates to ligands, homopolyligands, and heteropolyligands that modulate mTOR activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands and polyligands to a cellular localization signal, epitope tag and/or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.09-11-2008
20080220474GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST - The promoter regions associated with the 09-11-2008
20080220472In Vivo Incorporation of Alkynyl Amino Acids into Proteins in Eubacteria - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as 09-11-2008
20080220471Vectors and Methods Using Same - The present invention is directed to vectors and to methods of using same.09-11-2008
20130177941BABESIA MICROTI GENOMIC CLONES CONTAINING NOVEL ANTIGENS USEFUL IN THE DIAGNOSIS OF BABESIOSIS - Disclosed are the cloning and expression of novel antigens in 07-11-2013
20130177942Methods of Improving the Introduction of DNA into Bacterial Cells - The present invention relates to methods of improving the introduction of DNA into bacterial host cells.07-11-2013
20130177943NOVEL REGULATORY ELEMENTS - The invention concerns novel regulatory elements as well as related vectors and cells. Furthermore, it relates to methods of improving expression of polypeptides from nucleic acids such as cloned genes and to the production of various polypeptides in host cells using said novel regulatory elements. Additionally, the invention relates to uses of said novel regulatory elements as insulators, in gene therapy or for improving host cell lines.07-11-2013
20120252067Systems for the Expression of Orthogonal Translation Components in Eubacterial Host Cells - The invention related to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural acids at genetically-programmed positions.10-04-2012
20080213837Apo-3 ligand polypeptide - A tumor necrosis factor and lymphotoxin homolog having apoptotic activity, identified as Apo-3 Ligand, is provided. Nucleic acid molecules encoding Apo-3 Ligand, chimeric molecules and antibodies to Apo-3 Ligand are also provided.09-04-2008
20080213833Methods for Obtaining Optically Active Glycidyl Ethers and Optically Active Vicinal Diols from Racemic Substrates - The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific glycidyl ether hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active glycidyl ethers and associated optically active vicinal diols.09-04-2008
20090311745GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS - The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.12-17-2009
20130095525POLYNUCLEOTIDES HAVING LEADER SEQUENCE FUNCTION - The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.04-18-2013
20130115656ENGINEERED YEAST CELLS AND USES THEREOF - The present application provides engineered yeast cells and uses thereof. In specific embodiments, the yeast cells have a mutation in the GAL2 gene. In specific embodiments, the yeast cells can be used for producing a protein or compound of interest.05-09-2013
20130122543IMPROVED CELL CULTURE MEDIUM - The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.05-16-2013
20080233615NUCLEIC ACID ENCODING TWO-COMPONENT SENSING AND REGULATORY PROTEINS, ANTIMICROBIAL PROTEINS AND USES THEREFOR - Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells into which the expression vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.09-25-2008
20130130314Expression of Proteins in Plants - The invention relates to a method of producing an influenza virus H5 polypeptide in a plant comprising the steps of cloning an influenza H5 gene or nucleic acid encoding its functional equivalent into a vector adapted to target components present in the plant, infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to transiently express the influenza virus H5 polypeptide, and/or to create a transgenic plant; and recover the influenza virus H5 polypeptide expressed by the plant. The invention further relates to vectors, transgenic plants or parts thereof and the progeny of such plants used in or which come about as a result of the method.05-23-2013
20130130313METHOD OF SYNTHESIZING A SUPPRESSOR tRNA, DNA CONSTRUCT AND USE THEREOF FOR PRODUCING A NON-NATURAL AMINO ACID-INCORPORATED PROTEIN - There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same.05-23-2013
20090286281Glycosylation Variants of BACE - Human BACE polypeptides having modifications to the N-linked glycosylation sites including one or more of the following amino acid substitutions: S174I, N223A, N153Q and N354S. DNA sequences, vectors, and host cells for producing the polypeptides. Crystalline protein compositions formed from the purified polypeptides. Methods of screening for compounds that inhibit Aβ using the polypeptides.11-19-2009
20130143264HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS BY AMULTIPLE TRANSFECTION PROCEDURE OF MAR SEQUENCES - The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.06-06-2013
20130143265ZCYTOR17 HETERODIMERIC CYTOKINE RECEPTOR POLYNUCLEOTIDES - Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor17-containing multimeric or heterodimer cytokine receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. The present invention also includes methods for producing the multimeric or heterodimeric cytokine receptor, uses therefor and antibodies thereto.06-06-2013
20100279348YEAST STRAINS FOR PROTEIN PRODUCTION - Method and system for expression systems, based on ade1 and ade2 auxotrophic strains of yeast and fungi, including 11-04-2010
20080206814Method For Extracellular Production of Target Proteins By Co-Expression of Ompf and Target Proteins - The present invention relates to a method for secreting and producing a target protein into cell culture broth. More particularly, the invention relates to a microorganism co-transformed with a recombinant expression vector containing 08-28-2008
20080206813Means and methods for regulating gene expression - Described are means and methods for regulating gene expression and production of proteinaceous molecules. Described are methods for producing a proteinaceous molecule in a cell comprising selecting a cell for its suitability for producing the proteinaceous molecule, providing a nucleic acid encoding the proteinaceous molecule with a nucleic acid comprising a STAR (STabilizing Anti-Repression) sequence, expressing the resulting nucleic acid in the cell and collecting the proteinaceous molecule. Providing at least one STAR sequence to a nucleic acid encoding a proteinaceous molecule enhances production (yield) of the proteinaceous molecule by the host cell, increases the proportion of host cells with acceptable expression levels, and/or increases the stability of a gene expression level.08-28-2008
20080206812Methods for generating high titer helper-free preparations of released recombinant AAV vectors - This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus. Also provided is a quantitative, high-throughput assay useful in the assessment of viral infectivity and replication, as well as in the screening of agent that affect viral infectivity and/or replication.08-28-2008
20110217734COMPOSITIONS AND METHODS FOR THE SYNTHESIS OF APPA-CONTAINING PEPTIDES - The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.09-08-2011
20080199908Production Of Cellulase - The invention relates to a process of producing cellulase in a host cell, comprising cultivating said host cell capable of producing cellulase under conditions conducive for production of cellulase, wherein pre-treated ligno-cellulosic material is added to induce cellulase production. The invention also relates to use of pre-treated ligno-cellulosic material as inducer or carbon source in cellulase production processes.08-21-2008
20080199907Method For Preparing a Target Protein Using the Shsps - The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.08-21-2008
20080199906Optimized Nucleotide Sequences Encoding Sgp 130 - Described are codon optimized sgp130 encoding nucleic acid molecules as well as a method for the highly efficient recombinant production of sgp130 in mammalian cells or bacteria using a nucleic acid molecule of the invention.08-21-2008
20080199905Catalytic Domains Of Beta(1,4)-Galactosyltransferase I Having Altered Metal Ion Specificity - Disclosed are mutants of galactosyltransferases that can catalyze formation of oligosaccharides in the presence of magnesium; mutants of galactosyltransferases having altered donor and acceptor specificity which can catalyze formation of oligosaccharides in the presence of magnesium; methods and compositions that can be used to synthesize oligosaccharides; methods for increasing the immunogenicity of an antigen; and methods to stabilize platelets.08-21-2008
20110223636FLUORESCENT PROTEIN AND CHROMOPROTEIN - It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from 09-15-2011
20110223634TRANSFECTION KINETICS AND STRUCTURAL PROMOTERS - The invention features methods of analyzing the kinetics properties of transfection reactions. Also featured are methods for creating structural promoters which are effectively unregulated by enhancers and repressors. The structural promoters are significantly more active than the native promoter sequences upon which they are based.09-15-2011
20130137140INCREASED PROTEIN EXPRESSION THROUGH INCREASED MEMBRANE FORMATION - The present application relates to the field of protein expression technologies. More specifically, it is demonstrated that cells with increased levels of phosphatidic acid, which can be achieved through either or both of phosphatidic acid inhibition and diacylglycerol kinase overexpression, display increased membrane formation from which increased levels of proteins can be recovered.05-30-2013
20130149741METHODS FOR IMPROVED PRODUCTION OF BIOACTIVE WNT PROTEINS - Methods and compositions for protein expression are provided. In particular, cells producing efficient and reliable amounts of functional Wnt protein are provided.06-13-2013
20130149742FILAMENTOUS FUNGAL HOST STRAINS AND DNA CONSTRUCTS, AND METHODS OF USE THEREOF - The present disclosure relates to filamentous fungal host strains and recombinant DNA constructs for creation and use thereof. The filamentous fungal host strains are particularly useful for achieving reliable expression of recombinant enzymes and variants.06-13-2013
20130149743METHOD FOR ENHANCING LUMINESCENCE INTENSITY OF CLYTIN-II - A method for enhancing a luminescence activity of clytin-II is provided. A codon-optimized nucleic acid is used for coding the apo-clytin-II protein, and the luminescent activity of the clytin-II is remarkably enhanced when comparing with the conventional use of the wild-type clytin-II.06-13-2013
20110275121Methods and Compositions for Production of Recombinant Protein in HBX-Expressing Mammalian Cells - The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consisting of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method.11-10-2011
20100285526Compositions and Methods for Fusion Protein Separation - The present invention relates to compositions and methods for fusion protein separation utilizing a peptide linker comprising a novel thrombin cleavage site.11-11-2010
20100311121REGULATORY NUCLEIC ACID ELEMENTS - The invention relates to DNA-sequences, especially transcription- or expression-enhancing elements (TE elements) and their use on an expression vector in conjunction with an enhancer, a promoter, a product gene and a selectable marker.12-09-2010
20100317054PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF - The present invention relates to the cloning, identification and characterization of the unique and entire genomic sequences encoding new porcine DC-SIGN and LSECtin proteins, including the novel nucleotide sequences of the full-length cDNA and genes of both pDC-SIGN gene and pLSECtin. Also provided are the nucleic acid molecules encoding newly discovered porcine ICAM-3 isoforms from porcine monocyte-derived dendritic cells and the use thereof. Specifically, the invention is drawn to an isolated nucleic acid molecule comprising a nucleotide sequence encoding one or more of porcine DC-SIGN, porcine ICAM-3, porcine LSECtin, a complement of the nucleotide sequence or a functional, defined portion of the nucleotide sequence or a protein fusion product linked to a protein that may be of porcine or human origin. Methods for isolating and cloning the new porcine genes and for using the new nucleotide sequences in improved methods for propagating viruses, particularly enveloped viruses, are additionally described herein. The invention further includes new transfected cells or cell lines that can stably express the porcine proteins, new antibodies and the like.12-16-2010
20130157309Methods of obtaining genetic competence in bacillus cells - The present invention relates to methods of obtaining genetic competence in non-competent 06-20-2013
20130157308BIDIRECTIONAL PROMOTER REPORTER VECTOR FOR THE ANALYSIS OF DUAL REGULATORY ELEMENTS - A bidirectional expression vector is described that can be utilized to determine the existence and characteristics of bidirectional promoters. The bidirectional expression vector includes two different reporter genes in a head to head (5′ to 5′) arrangement. In addition, the bidirectional expression vector can include a polylinker region located between the heads of the two reporter genes that provides multiple cloning sites for nonexclusive examination of polynucleotide sequences. The vector can also include a splicing site and drug resistance. The bidirectional expression vector can be used to examine a polynucleotide sequence for the presence of divergent regulator regions and, following determination of a bidirectional promoter, can be utilized to further elucidate characteristics of the bidirectional promoter.06-20-2013
20130183713Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase Activity and Beta-Xylosidase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.07-18-2013
20100311116FAST GENERATION OF HIGH EXPRESSION STABLE CELL LINES EXPRESSING RECOMBINANT PROTEINS UNDER MINIMAL AND SHORT-TERM SELECTIVE PRESSURE - The present invention provides a novel method for the fast generation of high expression stable cell lines for the production of recombinant proteins with high efficacy of stable integration while using low selective pressure for only a short period of time. The method uses transiently expressed piggybac transposase to mediate stable integration of a transgene of interest flanked by the PB transposon termini.12-09-2010
20130122544ENZYME THAT CATALYZES A PEPTIDE-FORMING REACTION FROM A CARBOXY COMPONENT AND AN AMINE COMPONENT, MICROBE PRODUCING THE SAME, AND A METHOD OF PRODUCING A DIPEPTIDE USING THE ENZYME OR MICROBE - DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus 05-16-2013
20090104659NOVEL ALDOLASE AND PRODUCTION PROCESS OF 4-HYDROXY-L-ISOLEUCINE - A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus 04-23-2009
20110312031TRICHODERMA PROMOTER - A promoter for use in producing proteins in filamentous fungal host cells is provided. In one embodiment, the promoter comprises SEQ ID NO:1, or a variant or a truncated form thereof that has promoter activity in a host cell. Also provided are recombinant nucleic acids, vectors containing the promoter and host cells containing a recombinant nucleic acid or vector. Methods of producing a protein using the host cells are also provided.12-22-2011
20110312030CONSISTENT HIGH EXPRESSION VECTOR CONTAINING REP E MUTATED GENE - A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.12-22-2011
20110312029NOVEL REGULATORY ELEMENTS - The invention concerns novel regulatory elements as well as related vectors and cells. Furthermore, it relates to methods of improving expression of polypeptides from nucleic acids such as cloned genes and to the production of various polypeptides in host cells using said novel regulatory elements. Additionally, the invention relates to uses of said novel regulatory elements as insulators, in gene therapy or for improving host cell lines.12-22-2011
20110312028Novel Lipolytic Enzyme ELIP - The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.12-22-2011
20130189734Polypeptides Having Cellobiohydrolase Activitiy and Polynucleotides Encoding Same - The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.07-25-2013
20090263862Novel alkane polyol dehydrogenase - Disclosed is a protein selected from:10-22-2009
20120288893SEED-SPECIFIC EXPRESSION VECTOR AND ITS CONSTRUCTION METHODS AND APPLICATIONS - A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of 11-15-2012
20120028300Whitefly Ecdysone Receptor Nucleic Acids, Polypeptides, and Uses Thereof - The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.02-02-2012
20130196373MODIFIED DNA-BINDING PROTEINS AND USES THEREOF - Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.08-01-2013
20130196374CIS-ACTING ELEMENT AND USE THEREOF - The expression level of a desired gene is significantly improved when a filamentous fungus or the like is used as a host cell. The cis-acting element according to the present invention has a region in which the XlnR/Ace2-binding sequence (ggctaa) and the Hap complex-binding sequence (ccaat) are arranged with a spacer sequence of 0 to 100 nucleotides between them. Also, a transformant having the cis-acting element according to the present invention is cultured in a xylan-containing medium, for example, so that the expression level of a desired gene can be significantly improved.08-01-2013
20130203113METHOD OF STABILIZING mRNA - There is provided a method to increase the production of a desired protein in a microorganism by introduction of slowly translated codons in the encoding DNA gene sequence capable of slowing down the translation speed of the ribosomes moving along the mRNA, whereby the ribosomes protect the mRNA from being enzymatically degraded. This increases the stability of the mRNA transcript and thus results in an increased production of the desired protein. Moreover, there is provided a method of decreasing the half-life of a mRNA transcript from a gene encoding a peptide.08-08-2013
20130203114Regulation of Inducible Promoters - The present invention relates to the production of heterologous polypeptides in a recombinant bacterial host cell, wherein the bacterial host cell is rendered inable to deactivate the promoter controlling the expression of the heterologous polypeptide in the absence of an inducer.08-08-2013
20130095524METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME - The present invention provides a novel method of producing a recombinant bacterium for production of a non-natural protein, including: (1) expressing tRNA in a bacterium, which tRNA recognizes UAG codon; (2) expressing an aminoacyl-tRNA synthetase in the bacterium, which aminoacyl-tRNA synthetase acylates the tRNA with a non-natural amino acid or an α-hydroxy acid; (3) (i) introducing a DNA construct into the bacterium, which DNA construct is for expressing, in the absence of a release factor for terminating translation at UAG codon, a function of at least one gene selected from the group consisting of genes each of which loses its function when a gene that codes for the release factor is defective and/or introducing an alteration into said at least one gene in a chromosome of the bacterium, which alteration is for expressing the function of said at least one gene in the absence of the release factor; and (4) causing the gene that codes for the release factor in the bacterium to be defective.04-18-2013
20120083013GLUCOSYLTRANSFERASE SPECIFIC TO POSITION-4 OF FUROFURAN-TYPE LIGNAN, AND POLYNUCLEOTIDE ENCODING SAME - The invention provides a polynucleotide encoding a furofuran lignan 4-O-glucosyltransferase, and others. The polynucleotide in accordance with the present invention comprises a polynucleotide consisting of the nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 5, or a polynucleotide encoding a protein consisting of the amino acid sequence represented by SEQ ID NO: 4 or SEQ ID NO: 6.04-05-2012
20120088272METHODS OF GENERATING HYBRID/CHIMERIC CELLS, AND USES THEREOF - The present invention relates to hybrid cells and methods for producing hybrid cells. In particular, the invention relates to hybrid cells generated from the hybridization of at least three cells where at least two cells are derived from different lineages. The invention further relates to the use of hybrid cells for the expression of proteins useful in a range of diagnostic, prophylactic, therapeutic and/or research applications.04-12-2012
20120088271Use of an Endogenous 2-Micron Yeast Plasmid for Gene Over Expression - Methods and compositions for making stable recombinant yeast 2 μm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 μm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.04-12-2012
20120088270METHODS FOR PRODUCING ANTIFUNGAL BIFUNCTIONAL MOLECULES FOR TREATING FUNGAL INFECTION - The present invention is directed to methods of using nucleic acid molecules encoding for fusion peptides to produce the fusion peptides. The methods can include preparing or providing the nucleic acid molecules that having a fungal targeting agent (e.g., a fungal pheromone, such as alpha-mating pheromone) and a channel-forming domain consisting essentially of amino acids 451-626 of colicin Ia. The nucleic acid molecules can be transfected into host cells to produce the fusion peptide. The fusion peptides of the peptides of the present invention are particularly useful for the treatment of fungal infections in a wide variety of organisms.04-12-2012
20130210074EPIGENETIC ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines.08-15-2013