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Enzymatic production of a protein or polypeptide (e.g., enzymatic hydrolysis, etc.)

Subclass of:

435 - Chemistry: molecular biology and microbiology

435041000 - MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION

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DocumentTitleDate
20110183373RECOMBINANT PEPTIDE PRODUCTION USING A CROSS-LINKABLE SOLUBILITY TAG - The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.07-28-2011
20090130708DIPEPTIDE CRYSTALS AND PROCESS FOR PRODUCTION THEREOF - The present invention provides: (1) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent or a polypeptide consisting of three or more amino acids; and (2) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent, a polypeptide consisting of three or more amino acids, or an amino acid amide; and a process for producing the dipeptide crystals.05-21-2009
20100151515Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same - The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.06-17-2010
20110195450In Vitro Protein Synthesis Systems for Membrane Proteins that Include Adolipoproteins and Phospholipid Adolipoprotein Particles - In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.08-11-2011
20100081168Use of Acid Stable Protease in Animal Feed - The present invention relates to acid-stable proteases homologous to those derived from strains of the genus 04-01-2010
20100075374Methods for capturing nascent proteins - Methods of generating and capturing nascent proteins are described, including capturing nascent proteins on the same bead that comprises nucleic acid encoding the protein. The protein or nucleic acid can be sequenced before or after phototransfer to another surface.03-25-2010
20120244575CYCLOTIDE GENES IN THE FABACEAE PLANT FAMILY - The present invention relates to cyclotides and cyclotide-encoding genes from the Fabaceae plant family, and to the expression of cyclotides in Fabaceae. The present invention further relates to isolated nucleic acids configured to express cyclotides comprising heterologous peptide grafts in plants of the Fabaceae family.09-27-2012
20090246827RECOMBINANT TOXIN FRAGMENTS - A single polypeptide is provided which comprises first and second domains. The first domain enables the polypeptide to cleave one or more vesicle or plasma-membrane associated proteins essential to exocytosis, and the second domain enables the polypeptide to be translocated into a target cell or increases the solubility of the polypeptide, or both. The polypeptide thus combines useful properties of a clostridial toxin, such as a botulinum or tetanus toxin, without the toxicity associated with the natural molecule. The polypeptide can also contain a third domain that targets it to a specific cell, rendering the polypeptide useful in inhibition of exocytosis in target cells. Fusion proteins comprising the polypeptide, nucleic acids encoding the polypeptide and methods of making the polypeptide are also provided. Controlled activation of the polypeptide is possible and the polypeptide can be incorporated into vaccines and toxin assays.10-01-2009
20130084602SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS - The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating G04-04-2013
20090181423SUBSTANTIALLY ANIMAL PROTEIN-FREE RECOMBINANT FURIN AND METHODS FOR PRODUCING SAME - The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.07-16-2009
20120208232Cell-Free Synthesis of Active Reprogramming Transcription Factors - Compositions and methods are provided for the cell-free synthesis of active reprogramming factor polypeptides. The reprogramming factors may be synthesized as fusion proteins comprising a permeant domain, such as polyarginme. The cell free-synthesis may be conducted at about 25 C in a bacterial cell extract from genetically alterd cells having decreased endogenous protease activity Further, the proteins may comprise a fusion partner which enhances solubility and may be refolded on a column.08-16-2012
20100041096Carcinostatic method using BRCA1-BARD1 pathway - The present invention provides a method of polyubiquitinating a nucleophosmin comprised of reacting the nucleophosmin in vitro or in vivo with BRCA1-BARD1. The present invention also provides a method of inhibiting polyubiquitination of nucleophosmin comprised of phosphorylating BARD1 using CDK2-cyclin E and/or CDK2-cyclin A.02-18-2010
20090298118Enzymatic Conversion of Oligopeptide Amides to Oligopeptide Alkylesters - The present invention relates to enzymatic oligopeptide synthesis in the N→C direction, in particular to a process for the preparation of an optionally N-protected oligopeptide C-terminal alkylester comprising the step of reacting the corresponding optionally N-protected oligopeptide C-terminal carboxyamide with an alkyl alcohol, preferably methanol, in the presence of a peptide amidase. The formed C-terminal alkylester can subsequently be used for the coupling with another amino acid residue or oligopeptide. Therefore, inventors have found a very advantageous process for the preparation of oligopeptides.12-03-2009
20090123965Hydrolysis Process For Raw Materials From The Fishing And Slaughterhouse Industries And Tanks For Use Therein - Method for an enzymatic hydrolysis process of collagen and raw materials containing proteins. The raw materials undergo enzymatic hydrolysis to produce the three layers: a top layer containing fat, a mid layer comprising water soluble constituents, and a non-soluble bottom layer comprising bones and non-soluble proteins. These layers are separated and the second layer is further separated, in that it is cooled a time period sufficient for the forming of two layers: a bottom layer containing partially or wholly set collagen, and a liquid top layer containing the remaining water soluable proteins. The last layer is removed; and the other is heated until it becomes liquid. A hydrolysis tank 05-14-2009
20100267082NOVEL PEPTIDE-FORMING ENZYME GENE - DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus 10-21-2010
20120219988 COMPOSITION FOR POLYMERIZING A PROTEIN - The present invention provides a highly efficient method of polymerizing a protein and a composition of polymerizing a protein. For example, the present invention provides a method comprising contacting a tyrosinase derived from nameko mushroom (08-30-2012
20090253166 Conjugation of Peptides - Methods for the selective conjugation of peptides which comprises an enzymatic incorporation of a functional group at the C-terminal end of a peptide followed by reaction with a second compound comprising the moiety to be conjugated to the peptide, wherein said second compound comprises a functional group which selectively reacts with the incorporated functional group.10-08-2009
20130059333HEMATOPOIETIC CELL SELECTIN LIGAND POLYPEPTIDES AND METHODS OF USE THEREOF - The invention feature methods and compositions for treating inflammatory disorders, hematopoietic disorders and non-hematopoietic disorders (e.g., non-hematopoietic cancers) and for isolating cells (e.g., stem cells) in a mammal.03-07-2013
20120190064FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES - Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.07-26-2012
20130065271Host Cells and Culture Methods - Improved host cells and culture methods involving overexpression of MAN1C1 activity to improve protein production are provided.03-14-2013
20130065272Synthesis of Site Specifically-Linked Ubiquitin - The invention relates to a method of modifying a specific lysine residue in a polypeptide comprising at least two lysine residues, said method comprising (a) providing a polypeptide comprising a target lysine residue protected by a first protecting group, and at least one further lysine residue; (b) treating the polypeptide to protect said further lysine residue(s), wherein the protecting group for said further lysine residues is different to the protecting group for the target lysine residue; (c) selectively deprotecting the target lysine residue; and (d) modifying the deprotected lysine residue of (c).03-14-2013
20130065273Engineered E2 For Increasing The Content Of Free LYS11-Linked Ubiquitin - The invention provides a chimeric E2 enzyme comprising a Ubc domain fused to a heterologous ubiquitin binding domain (UBD). The chimeric enzymes of the invention may be useful in producing elevated levels of free polyubiquitin.03-14-2013
20110020864Preparation of High Purity Collagen - A method of preparing collagen by first producing a collagen matrix and then extracting collagen from the matrix.01-27-2011
20090011459Process for producing functional non-naturally occurring proteins, and method for site-specific modification and immobilization of the proteins - There is provided a process for industrial production of non-naturally occurring proteins composed of less than 20 amino acids, wherein the proteins retain their original functions while being capable of site-specific modification or immobilization, or having new functions not found in nature in addition to the original functions of the proteins.01-08-2009
20100086966FEEDBACK RESISTANT ACETOHYDROXY ACID SYNTHETHASE MUTANTS - The present invention provides nucleotide sequences coding for acetohydroxy acid synthetase (AHAS) mutants, the mutated enzymes themselves and a process for the fermentative production of branched-chain amino acids using these enzymes in specific hosts in which genes which code for the modified acetohydroxy acid synthetase (AHAS) are expressed.04-08-2010
20120237971METHODS, CELLS AND SYSTEMS FOR INCORPORATING NON-CANONICAL AMINO ACIDS INTO PROTEINS - The present disclosure pertains to methods of incorporating one or more non-canonical amino acids into a protein during a translation of the protein in bacterial cells. The present disclosure also pertains to methods of incorporating one or more non-canonical amino acids into a protein during an in vitro translation of the protein. In additional embodiments, the present disclosure pertains to isolated bacterial cells and in vitro translation systems (e.g., cell-free extract systems) for incorporating one or more non-canonical amino acids into a protein during a translation of the protein.09-20-2012
20110033889APPARATUS AND METHOD FOR HYDROLYSIS OF A PROTEIN CONTAINING RAW MATERIAL AND APPLICATION OF THE RESULTING HYDROLYSIS PRODUCTS - Apparatus and methods for hydrolyzing protein-containing raw material into water soluble protein and other products. The apparatuses and methods comprise an optional collection or processing stage in which protein-containing raw material, such as fish or animal carcasses from food production plants, are collected and optionally processed. The raw material is then reacted with one or more enzymes to hydrolyze the protein present, after which the one or more enzymes are inactivated and the components separated. The processes and apparatuses, which can be run as a batch processes or, advantageously as a continuous processes, can yield water soluble protein, oils, bone meal and other products that have utility as food or food additives.02-10-2011
20110081678METHOD FOR PRODUCING BETA-ALANYL-AMINO ACID OR DERIVATIVE THEREOF - The present invention provides a method for efficiently producing a β-alanyl-amino acid (e.g., carnosine) or derivative thereof. Specifically, it provides a method for producing a β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof, by reacting a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof in the presence of an enzyme or an enzyme-containing product that has an ability to form the β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof from the β-alanyl ester or the β-alanyl amide and the amino acid (e.g., histidine) or derivative thereof. The present invention also provides a protein which is able to catalyze production of a β-alanyl-amino acid or derivative thereof from a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof, and a polynucleotide encoding said protein.04-07-2011
20110159537SYNTHETIC PATHWAY ENZYMES FOR THE PRODUCTION OF ARGYRINS - The invention provides the amino acid sequences comprised in or constituting the synthetic pathway enzymes participating in the production of Argyrins, as well as the nucleic acid sequences encoding the synthetic pathway enzymes participating in the production of Argyrins, as well as genetically manipulated micro-organisms containing nucleic acid sequences encoding the synthetic pathway enzymes for the production of Argyrins, e.g. for inserting one or more of these coding sequences, mutating in a targeted manner one or more of these nucleic acid sequences, in a wild type producer micro-organism or in a heterologous micro-organism, for the production of Argyrins.06-30-2011
20100216184PREPARATION OF CELL EXTRACT AND ITS APPLICATION FOR CELL-FREE PROTEIN SYSTHESIS - Disclosed is a process for simply preparing cell extracts for use as a catalyst of cell-free protein synthesis by centrifugation, which improves cost effectiveness and productivity of cell-free protein synthesis. Specifically, a conventional process for preparing cell extracts comprises the complicated steps, i.e. cell culture, cell lysis, high-speed centrifugation, pre-incubation, dialysis and the like. In comparison, the cell lysate just obtained by centrifugation is directly applied to protein synthesis, thereby providing higher producibility and more consistent productivity of protein than the conventional process. Further, the cell extracts are prepared by the simple process to reduce the protein production cost and time by about 60% and about 80%, respectively.08-26-2010
20100184132Enzymatic Process for Debittering of Protein Hydrolysate Using Immobilized Peptidases - A method for enzymatic debittering of protein hydrolysates comprising the steps of isolating the protein hydrolysates from animal and plant source, reacting the said protein hydrolysates with peptidases immobilized on calcium alginate beads packed in a column.07-22-2010
20110318780ENZYMATIC MODIFICATION OF GLYCOPEPTIDES - The present invention provides glycoconjugates that are formed through the enzymatically-mediated coupling of a glycosyl moiety, e.g., on a peptide or lipid, and a modifying group that includes an acyl group. The conjugates include the modifying group tethered to the glycosyl moiety through a linking moiety that includes an acyl residue. Also provided are methods for preparing the conjugates of the invention12-29-2011
20110287478Protein concentrates and isolates, and processes for the production thereof - Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from an oilseed meal using low g-force centrifugation.11-24-2011
20110287479PROTEASE CATALYZED IN SITU END CAPPING OF OLIGOPEPTIDES IN AQUEOUS MEDIA - One-pot biotransformations give oligo(γ-11-24-2011
20090093016Selective Enzymatic Hydrolysis of C-Terminal Tert-Butyl Esters of Peptides - The present invention relates to a process for the selective enzymatic hydrolysis of C-terminal esters of peptide substrates in the synthesis of peptides, comprising hydrolysing C-terminal tert-butyl esters using the protease subtilisin. This process is useful in the production of protected or unprotected peptides.04-09-2009
20090253165METHOD FOR PREPARING MICROCAPSULES BY COACERVATION - The present invention relates to a method for preparing microcapsules by coacervation, and to the use of transglutaminase for cross-linking in complex coacervation. The present invention relates further to coacervation processes in general in which a material to be encapsulated is added to a solution comprising at least one colloid below the gelling temperature of the colloid. According to a method of the present invention, an emulsion or suspension of hydrophobic material is prepared after cooling a solution that includes hydrocolloids below the critical gelling temperature of a coacervate phase.10-08-2009
20090035816Process for the preparation of a polypeptide - A process for the preparation of a polypeptide made from amino acids L-alanine, L-glutamic acid, L-lysine, and L-tyrosine comprising using N-thiocarboxyanhydride of at least one amino acid as a starting material.02-05-2009
20090035813OLIGOSACCHARIDE MIXTURE - An oligosaccharide mixture derived from animal milk, food products comprising said oligosaccharide mixture and a process for producing said oligosaccharide mixture.02-05-2009
20110287477Protein concentrates and isolates, and processes for the production thereof from toasted oilseed meal - Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from a toasted oilseed meal using low g-force centrifugation.11-24-2011
20110076719Substantially Animal Protein-Free Recombinant Furin and Methods for Producing the Same - The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.03-31-2011
20110300575CELL-FREE SYSTEM FOR SYNTHESIS OF PROTEINS DERIVED FROM CULTURED MAMMALIAN CELLS - Prepared is an extract composition having an improved protein synthetic activity in a cell-free protein synthesis system using a mammalian cultured cell extract. An eukaryotic translation initiation factor and/or translational regulator are added to a cell-free protein synthesis system comprising an extract prepared from cultured mammalian cells and a template mRNA. These factors are one or more selected from the group consisting of eukaryotic translation initiation factors 4E (eIF4E), 2 (eIF2) and 2B (eIF2B), and eukaryotic translational regulator p97.12-08-2011
20090286279Genetically programmed expression of selectively sulfated proteins in eubacteria - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as 11-19-2009
20100304430Methods for Generating Analogs of Coenzyme A - Methods to generate analogs of coenzyme A in vivo are disclosed. The methods to generate analogs of coenzyme A in a cell comprise reacting pantetheine or a derivative thereof with a reporter to form labeled pantetheine or a derivative thereof, contacting the cell with the labeled pantetheine or derivative thereof such that the labeled pantetheine or derivative thereof enters the cell, phosphorylating the labeled pantetheine or derivative thereof to form phosphopantetheine or a derivative thereof, adenylating the labeled phosphopantetheine or derivative thereof to form a labeled dephosphoCoenzyme A or derivative thereof, and phosphorylating the 3′-hydroxyl of the labeled dephosphoCoenzyme A or derivative thereof to form a labeled coenzyme A analog or derivative thereof.12-02-2010
20090029413Process for the manufacture of a composite material - The invention relates to a process for the manufacture of a composite materials comprising the steps of 01-29-2009
20100035299METHODS FOR THE PURIFICATION OF POLYPEPTIDE CONJUGATES - The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.02-11-2010
20090148888RECOMBINANT TOXIN FRAGMENTS - A single polypeptide is provided which comprises first and second domains. The first domain enables the polypeptide to cleave one or more vesicle or plasma-membrane associated proteins essential to exocytosis, and the second domain enables the polypeptide to be translocated into a target cell or increases the solubility of the polypeptide, or both. The polypeptide thus combines useful properties of a clostridial toxin, such as a botulinum or tetanus toxin, without the toxicity associated with the natural molecule. The polypeptide can also contain a third domain that targets it to a specific cell, rendering the polypeptide useful in inhibition of exocytosis in target cells. Fusion proteins comprising the polypeptide, nucleic acids encoding the polypeptide and methods of making the polypeptide are also provided. Controlled activation of the polypeptide is possible and the polypeptide can be incorporated into vaccines and toxin assays.06-11-2009
20120142048NUCLEIC ACID WHICH IS STABILIZED AGAINST DECOMPOSITION - The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optimally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.06-07-2012
20090209000Genetically Programmed Expression of Proteins - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as 08-20-2009
20120107869ORGANIC COMPOUNDS - Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.05-03-2012
20120107868PROCESSES FOR MAKING PROTEIN HYDROLYSATES FROM ANIMAL PEPTONE AND FOR PRESERVING MUCOSA - A process for hydrolyzing products with enzymatic activity remaining in peptone solutions after mucosa hydrolysis is provided along with a process for preserving mucosa tissue. Broadly, the processes are carried out by hydrolyzing mucosa tissue according to conventional heparin manufacturing processes wherein an excess quantity of proteolytic enzymes is used. The resulting peptone solution is then contacted with proteins or protein-containing materials in order to hydrolyze the proteins. In another embodiment, mucosa tissue is preserved by mixing it with a preserving agent selected from the group consisting of hydrogen peroxide and phosphoric acid. The product preserved by hydrogen peroxide is low in ash, stable for at least a week, and has a reduced odor.05-03-2012
20120107867GLYCOPEGYLATED ERYTHROPOIETIN - The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.05-03-2012
20090263858Process for synthesis of mucin-type peptides and muc1-related glycopeptides - The invention aims at providing novel compounds useful as primer in producing mucin-type glycopeptides which are useful in a wide field including materials for biochemical research, drugs, and food and the production of which was difficult in the prior art; and a process for the production of glycopeptides by using the primers. The aim is attained by providing novel glycopeptide derivatives (represented by the general formula (I)) which each bear an aldehyde or ketone group at the end and each contain an amino acid residue cleavable with a protease; and an easy and simple process for the production of glycopeptides by using the derivative as the primer.10-22-2009
20100099142Processing of Peptides and Proteins - The invention provides novel methionine aminopeptidase enzymes and their use.04-22-2010
20090258387BIOACTIVE WHEY PROTEIN HYDROLYSATE - The invention relates to a partial hydrolysate of when protein which contains bioactive peptides but does not have a bitter flavour. The hydrolysate is carried out using selective enzymes which produce the active peptides and is terminated at a degree of hydrolysis before substantial bitter flavours are created. There are also described novel peptides and a method of reducing systolic blood pressure through the administration of the peptides.10-15-2009
20120295304METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER - A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.11-22-2012
20100112635Protein hydrolysates enriched in peptides having a carboxy terminal proline residue - A method of enzymatically producing a protein hydrolysate from a protein substrate is described, wherein a proline-specific endoprotease or a composition containing a proline-specific endoprotease and optionally a subtilisin or a metallo endoprotease, and other enzymes such as carboxypeptidases, is used to produce a protein hydrolysate enriched in peptide fragments having a carboxy-terminal proline residue. Such protein hydrolysates may be used as such or to reduce bitterness in foods nutritionally supplemented by protein hydrolysates, as well as to produce hydrolysate-containing foodstuffs having low antigenicity.05-06-2010
20100136615METHOD OF PRODUCING SOYBEAN PROTEIN MATERIAL - It is intended to provide a soybean protein material which is highly dispersible in hot water and highly miscible with water so as to give a solution showing regulated coarseness. More specifically, it is intended to provide a soybean protein material which is appropriately usable in preparing protein drinks. According to a method comprising the following steps, it becomes possible to produce a soybean protein material which is dispersible in hot water and miscible with water: the step wherein soybean protein is hydrolyzed with a protease in an aqueous system until the 0.22 MTCA solubility attains 15 to 30%; the step wherein an emulsifier having an HLB value of 6 or more but not more than 13 is added to the soybean protein; the step of drying the soybean protein after the hydrolysis; and the step of heating the soybean protein in an aqueous system at 105 to 180° C. for 15 to 180 seconds before the drying.06-03-2010
20100143969EXPRESSION OF SOLUBLE, ACTIVE EUKARYOTIC GLYCOSYLTRANSFERASES IN PROKARYOTIC ORGANISMS - The present invention provides enhanced methods of producing soluble, active eukaryotic glycosyltransferases in prokaryotic microorganisms that have an oxidizing environment.06-10-2010
20080305519Biochemical method for specific protein labeling - An improved method for protein labeling comprising the steps of providing a synthetic small molecule tag, providing a target protein to be tagged, providing at least two enzymes for catalyzing a conjugation reaction between the tag and the target protein, incubating the tag, the protein and the enzyme, and allowing the tag to conjugate to the target protein. The tag may embody at least one structural feature of an ubiquitin C-terminus, and the structural feature may comprise a recognition sequence that is recognizable by an ubiquitin activating enzyme.12-11-2008
20080299607Enzymatic Process To Produce Highly Functional Soy Protein from Crude Soy Material - This invention relates generally to the processing of soy-derived materials for use in various products. More particularly, the invention relates to a process producing highly functional soy protein using ultrafiltration followed by an enzymatic treatment.12-04-2008
20120295305Cysteine Protease Autoprocessing of Fusion Proteins - Disclosed are fusion proteins, polynucleotides that encode the disclosed fusion proteins, and methods for expressing and autoprocessing of the disclosed fusion proteins to obtain a target protein. The disclosed fusion proteins include an autoproteolytic cysteine protease fused to a heterologous polypeptide, which may be isolated as the target protein. Preferably, the protease activity of the cysteine protease is inducible. Suitable autoproteolytic cysteine proteases for the fusion proteins include the cysteine protease of the 11-22-2012
20090004692METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.01-01-2009
20080268498Thermostable Viral Polymerases and Methods of Use - Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and/or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.10-30-2008
20090053760SELECTIVE ENZYMATIC AMIDATION OF C-TERMINAL ESTERS OR ACIDS OF PEPTIDES - The present invention relates to a process for the amidation of C-terminal esters or acids of peptide substrates in solution-phase synthesis of peptides, comprising amidating one or more peptide substrates comprising C-terminal esters or acids using the protease subtilisin in any suitable form in the presence of an ammonium salt derived from an acid having a pKa above 0.02-26-2009
20090029414CELL-FREE SYNTHESIS OF MEMBRANE BOUND POLYPEPTIDES - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane- associated polypeptides.01-29-2009
20090029412SOLUBILITY TAGS FOR THE EXPRESSION AND PURIFICATION OF BIOACTIVE PEPTIDES - Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.01-29-2009
20090029411Biomaterial Capable Of Successively Performing A Solution/Gel Transition And Then A Gel/Solution Transition - The invention relates to a biomaterial which can successively carry out a solution/gel transition then a gel/solution transition with given kinetics, said biomaterial comprising at least one monomer which may form polymers, at least on enzyme which may decompose said polymers and, optionally, an enzyme which may form covalent bonds between said monomers. The invention further relates to a method for the production of said biomaterial.01-29-2009
20090136993Process for the Production of Gamma-Glutamylcysteine - The present invention relates to a process for preparing a compound comprising an α,γ amide linkage between a cysteine moiety and a glutamic acid moiety, such as γ-glutamylcysteine or a γ-glutamylcysteine derivative, the process comprising providing a cysteine derivative, a γ-glutamyl donor and an enzyme capable of transferring the γ-glutamyl group to said cysteine derivative in a reaction environment promoting transfer of the γ-glutamyl donor and cysteine derivative. The invention also relates to compounds comprising an α,γ amide linkage between a cysteine moiety and a glutamic acid moiety, such as γ-glutamylcysteine or a γ-glutamylcysteine derivative, when obtained by processes of the invention, and uses thereof.05-28-2009
20090047705MICROORGANISM-DERIVED PSYCHROPHILIC ENDONUCLEASE - A polypeptide having an endonuclease activity derived from a psychrophilic microorganism 02-19-2009
20090191586ENZYME EXPRESSION METHODS AND COMPOSITIONS - Methods for expressing active enzymes are described that involve co-expressing a first enzyme with a second enzyme that has an enzymatic activity that reverses a modification on the first enzyme and/or for identification of soluble and/or active catalytic domains by systematic variation of fragment lengths around catalytic domain boundaries.07-30-2009
20090203068High Pressure Enzymatic Digestion System For Protein Characterization - A method and system for obtaining samples for proteomic analysis that utilizes pressure and a preselected agent to obtain a processing sample in a significantly shorter period of time than prior art methods and which maintains the integrity of the processing sample through the preparatory process. In one embodiment of the invention, a sample and an enzyme are combined and subjected to a pressure, preferably a pressure cycle range that varies between 0 to 35 kpsi, for a period of time of preferably less than 60 seconds. This process results in producing a sample suitable for analysis, which is preferably introduced to another analytical instrument such as a mass spectrometry instrument, or other device.08-13-2009
20090239258Anti-IL-6 Antibody Nucleic Acid Molecules and Methods - Anti-IL-6 antibody nucleic acids, vectors, host cells, transgenic animals or plants, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices.09-24-2009
20090275077Methods of Obtaining Optically Active Epoxides and Vicinal Diols from Styrene Oxides - The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific meso-epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining 11-05-2009
20100151514NOVEL MUCIN-TYPE GLYCOPROTEIN AND USE THEREOF - Provided is a novel mucin-type glycoprotein and a method for producing the same. Specifically, a mucin-type glycoprotein having a repeat structure including 3 to 2000 repeating units each having an amino acid sequence represented by the formula I: Val-Xaa-Glu-Thr-Thr-Ala-Ala-Pro [wherein Xaa represents Val or Ile] (SEQ ID NO: 1), wherein one or more amino acid residues in the structure are bound to a sugar chain of one or more monosaccharides. Also provided is a composition containing the novel mucin-type glycoprotein. Further provided is a molecular weight marker containing the novel mucin-type glycoprotein.06-17-2010
20100184134DUAL CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO SYNTHESIS METHOD - This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.07-22-2010
20130122542MODIFIED SAK GENE FOR THE PRODUCTION OF RECOMBINANT PROTEINS - The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant heterologous proteins from bacterial inclusion bodies using modified SAK.05-16-2013
20100184133Method for making maturated insulin polypeptides - The invention is related to a method for making mature human insulin or an analogue thereof by culturing a fungi cell comprising a DNA sequence encoding a precursor for human insulin or an analogue of human insulin which precursor comprises the B-chain of human insulin or an analogue thereof, the A-chain of human insulin or an analogue thereof and a C-peptide linking the B-chain and the A-chain together thereof, wherein the C-peptide comprises at least one Kex2 cleavage site and an amino acid sequence attached at one end to the C-terminal amino acid residue in the B-chain and at the other end to the Kex2 site which amino acid sequence will facilitate a more efficient Kex2 cleavage within the fungi cell. The C-terminal extension of the B-chain may furthermore be capable of subsequently being cleaved off from the C-terminal amino acid residue in the B-chain by means of a carboxypeptidase activity either within the fungi cell or subsequently in the culture medium.07-22-2010
20100196951Antitoxin Destabilization Technology - Disclosed are methods for purifying toxin proteins which avoid denaturation and renaturation procedures.08-05-2010
20100173357METHOD OF MAKING ACTIVATED CARBOXYPEPTIDASES - The invention is related to a method for making an activated carboxypeptidase in a fungi cell comprising introducing a DNA sequence encoding a proform of the carboxypeptidase wherein a Kex2 site has been introduced in the prosequence of the carboxypeptidase, culturing the fungi cell under conditions suitable for expression of the procarboxypeptidase and cleaving off the prosequence within the cell to liberate the free active form of the carboxypeptidase. The invention is also related to methods for making mature human insulin and human insulin analogues by use of the activated carboxypeptidase enzyme.07-08-2010
20130217067Combinatorial DNA Library for Producing Modified N-Glycans In Lower Eukaryotes - The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man08-22-2013
20090280526Method for in Vitro Molecular Evolution of Protein Function - The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of 11-12-2009
20090075329CHEMICALLY MODIFIED MUTANT SERINE HYDROLASES SHOW IMPROVED CATALYTIC ACTIVITY AND CHIRAL SELECTIVITY - This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C03-19-2009
20110236925Method of Obtaining a Purified, Biologically Active Heterologous Protein - The invention relates to methods of separation and/or purification of impurities yielding a purified heterologous protein product devoid of related impurities or with substantially minimal quantities of such glycosylated impurities. More specifically, the invention relates to the identification of glycosylated forms of insulin analogues such as glargine impurities characterized post expression in yeast based systems such as 09-29-2011
20100196952METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, without separating generated substances and without adding consumed synthesis substances within the defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products and/or reaction inhibitors are separated from the solution (and extracted), d) immediately before, after or at the same time as step c) consumed synthesis substances are supplemented, e) steps b), c) and d) are repeated at least once with the reaction solution of step d), and at the last execution of step b) steps c) and d) may be left out.08-05-2010
20110151510Functional molecule and manufacturing method therefor - A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.06-23-2011
20110111455Polymer-Von Willebrand Factor-Conjugates - The present invention relates to a proteinaceous construct (also designated as polymer-VWF-conjugate) comprising plasmatic and/or recombinant von Willebrand factor (VWF), said VWF being bound to at least one physiologically acceptable polymer molecule, as well as to a complex between said proteinaceous construct and at least one factor VIII (FVIII) protein. The physiologically acceptable polymer molecule can be, for instance, polyethylene glycol (PEG) or polysialic acid (PSA). Further the present invention relates to methods for prolonging the in vivo-half-life of VWF or FVIII in the blood of a mammal having a bleeding disorder associated with functional defects of or deficiencies of at least one of FVIII or VWF.05-12-2011
20110111456PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.05-12-2011
20100311112METHOD FOR AMIDATING POLYPEPTIDES WITH BASIC AMINO ACID C-TERMINALS BY MEANS OF SPECIFIC ENDOPROTEASES - The invention relates to a method for producing C-terminal amidated dibasic or polybasic peptides, consisting in reacting two peptides in the presence of trypsin biologically active enzymes and, if necessary, in purifying the thus obtainable compounds of formula (I) by means of protein chemistry.12-09-2010
20100311113CHEMO-ENZYMATIC PEPTIDE SYNTHESIS VIA C-TERMINAL ESTER INTERCONVERSION - The invention relates to a method for preparing an optionally N-protected amino acid C-terminal ester or an optionally N-protected peptide C-terminal ester, comprising transesterifying the C-terminal t-alkyl ester of the amino acid or the C-terminal t-alkyl ester of the peptide with an alcohol (other than the t-alcohol corresponding to the t-alkyl group of the ester) in the presence of a hydrolytic enzyme (E.C. 3).12-09-2010
20110033890METHOD FOR THE SECRETORY PRODUCTION OF HETEROLOGOUS PROTEIN IN ESCHERICHIA COLI - The present invention relates to a signal sequence peptide for the improvement of extracellular secretion efficiency of a heterologous protein in 02-10-2011
20090035815Synthetic Gene for Enhanced Expression in E. Coli - A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for 02-05-2009
20090186377MODIFICATIONS OF CST-II FOR INCREASED PROTEIN EXPRESSION - The present invention provides modified 07-23-2009
20110008829Use of Synthetic Scaffolds for the Production of Biosynthetic Pathway Products - The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.01-13-2011
20110008830PROCESS FOR PRODUCING BIO-GEL AND A BIO-GEL - Provided is a process for producing an enzyme-containing bio-gel from an enzyme solution, particularly a commercial α-amylase solution. When allowed to stand in a vessel, enzyme from the enzyme solution collects on the inside surface of the vessel, thus forming a bio-gel. The bio-gel is recovered by removing the enzyme solution, then collecting, and optionally drying, the material deposited on the inner surface of the vessel. A bio-gel produced by this process is also provided.01-13-2011
20110008828METHODS OF INCORPORATING AMINO ACID ANALOGS INTO PROTEINS - The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.01-13-2011
20110111454ENGINEERED VERSIONS OF CgtB (Beta-1,3 GALACTOSYLTRANSFERASE) ENZYMES, WITH ENHANCED ENZYMATIC PROPERTIES - CgtB proteins with enhanced beta 1,3-galactosaminyltransferase activity, nucleic acids that encode the CgtB proteins and methods for use of the CgtB proteins.05-12-2011
20120034650Nucleic acid molecule of a biosynthetic cluster encoding non ribosomal peptide synthases and uses thereof - The present invention relates to the provision of a polynucleotide comprising one or more functional fragments of a biosynthetic gene cluster involved in the production of a compound of formula (I) or (I′). The present invention also provides a method of preparing a compound of formula (I) or (I′) or of formula (II) to (VII), (XI) to (XIV) and (XVII) and (XVIII). Moreover, the use of such compound as a pharmaceutical composition is also provided in the present invention.02-09-2012
20100015659PC5 as a Factor IX Propeptide Processing Enzyme - Compositions and methods for preparing Factor IX, Factor IX-containing fusion proteins, and Factor IX-containing conjugates with processing of Factor IX propeptide by PC5, are provided. In one embodiment PC5 is used to process a precursor polypeptide for a Factor IX-Fc monomer-dimer hybrid.01-21-2010
20100055737Cyclodipeptide Synthetase and its use for Synthesis of Cyclo(Tyr-Xaa) Cyclodipeptides - Isolated, natural or synthetic polynucleotide and polypeptide encoded by said polynucleotide, that is involved in the synthesis of cyclodipeptides, recombinant vector comprising said polynucleotide or any substantially homologous polynucleotide, host cell modified with said polynucleotide or said recombinant vector and also methods for in vitro and in vivo synthesizing cyclodipeptides, in particular cyclo(Tyr-Xaa) cyclodipeptides, wherein Xaa is any amino acid and their derivatives and applications thereof.03-04-2010
20110250638LIGNOCELLULOSIC BIOMASS CONVERSION - The present invention relates to a process for the production of second generation biofuels and/or sugar based chemicals—for example ethanol, butanol etc—and/or materials—for example plastics, single cell proteins etc.—together with sulfonated lignin from lignocellulosic biomass, in particular from lignocellulosic biomass comprising, among others, annual plants, agricultural waste, or wood. In particular, the present invention relates to a process for the production of sugar based chemicals, biofuels or materials together with sulfonated lignin from lignocellulosic biomass comprising the pretreatment of a lignocellulosic biomass in a sulfite cooking step.10-13-2011
20110177552SYSTEMS AND METHODS FOR PRODUCING PLASTID PROTEINS - Methods and systems that include a method comprising: providing a system comprising one or more plastid proteins comprising a signal sequence and one or more chloroplast processing enzymes; and allowing at least one of the one or more chloroplast processing enzymes to cleave at least a portion of a signal sequence from at least one of the one or more plastid proteins.07-21-2011
20090215113Methods for purifying pertussis toxin and peptides useful therefor - The present invention relates to reagents and methods for purifying pertussis toxin (PT).08-27-2009
20110097760Method For Producing a Casein Hydrolysate - The present invention relates to a method for producing a casein hydrolysate using a microbial endopeptidase.04-28-2011
20100129861Isolation and Use of Novel Mammalian DExH Box Helicases - The present invention relates to the isolation, purification, and use of novel mammalian DExH box helicases. In particular, the present invention relates to the isolation, purification and use of DHX29, a novel mammalian RNA helicase.05-27-2010
20110070607GLYCOPROTEIN SYNTHESIS AND REMODELING BY ENZYMATIC TRANSGLYCOSYLATION - A chemoenzymatic method for the preparation of a homogeneous glycoprotein or glycopeptide, including (a) providing an acceptor selected from the group consisting of GlcNAc-protein and GlcNAc-peptide; and (b) reacting the acceptor with a donor substrate including an activated oligosaccharide moiety, in the presence of a catalyst comprising endoglycosidase (ENGase), to transfer the oligosaccharide moiety to the acceptor and yield the homogeneous glycoprotein or glycopeptide. The donor substrate includes, in a specific implementation, a synthetic oligosaccharide oxazoline. A related method of glycoprotein or glycopeptide remodeling with a predetermined natural N-glycan or a tailor-made oligosaccharide moiety, and a method of remodeling an antibody including a heterogeneous sugar chain, are also described. The disclosed methodology enables glycoprotein drugs to be modified for prolonged half-life in vivo, reduced immunogenicity, and enhanced in vivo activity, and for targeting and drug delivery.03-24-2011
20100311114PREPARATION OF SAMPLES FOR PROTEOME ANALYSIS - The invention mainly concerns methods and systems for the preparation of biological samples for proteome analysis, N-terminal or C-terminal peptides of proteins are enriched using exopeptidase.12-09-2010
20100311115SELECTIVE ENRICHMENT OF POST-TRANSLATIONALLY MODIFIED PROTEINS - The present invention relates to the selective enrichment of post-translationally modified proteins and/or peptides from complex samples. In particular, the invention relates to methods for the separation of phospho-proteins and/or -peptides and/or for the discrimination between different subsets of phospho-proteins and/or -peptides.12-09-2010
20080248522HIGH-PRESSURE INCLUSION BODY SOLUBILIZATION AND PROTEASE CLIPPING OF RECOMBINANT FUSION PROTEINS - Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.10-09-2008
20080248521ENHANCED CELL-FREE SYNTHESIS OF ACTIVE PROTEINS CONTAINING DISULFIDE BONDS - Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.10-09-2008
20090317861CELL-FREE SYNTHESIS OF VIRUS LIKE PARTICLES - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of virus like particles.12-24-2009
20080206808GALACTOSYL NUCLEOTIDE SUGAR - The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.08-28-2008
20120064568ENDOMANNOSIDASES IN THE MODIFICATION OF GLYCOPROTEINS IN EUKARYOTES - The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.03-15-2012
20080213828Glycoprotein synthesis - Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.09-04-2008
20080213827Process For Producing Dipeptides or Dipeptide Derivatives - The present invention provides a process for producing a dipeptide or a dipeptide derivative by using a protein having the activity to form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture as a enzyme source, which comprise;09-04-2008
20100297693Production of carrier-peptide conjugates using chemically reactive unnatural amino acids - Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.11-25-2010
20080254505WHEY PROTEIN HYDROLYSATE - The present invention relates to a method of producing a whey protein hydrolysate using a microbial endopeptidase which specifically cleaves on the carboxy terminal side of arginine or lysine. The invention also relates to use of such whey protein hydrolysate in sports drinks or in clinical nutrition.10-16-2008
20120202243 IN Vivo Incorporation of Unnatural Amino Acids - The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.08-09-2012
20110053215Endoglycosidases that Cleave O-linked Glycans - Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.03-03-2011
20110053214PRODUCTION OF GALACTOSYLATED GLYCOPROTEINS IN LOWER EUKARYOTES - The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.03-03-2011
20110027829Methods and Compositions - The invention relates to a tRNA synthetase capable of binding N02-03-2011
20100285525METHOD - A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses one or both of the following reactions: alcoholysis or transesterification.11-11-2010
20110136168PROCESS FOR PRODUCTION OF NON-NATURAL PROTEIN HAVING ESTER BOND THEREIN - A non-natural protein having at least one ester bond in its polypeptide main chain is synthesized by using an in vivo translation system in a ribosome. The following components (a) to (c) are expressed in a cell or an cell extraction solution in the presence of an α-hydroxy acid: (a) an aminoacyl-tRNA synthetase which can activate the α-hydroxy acid; (b) suppressor tRNA which can bind to the α-hydroxy acid in the presence of the aminoacyl-tRNA synthetase; and (c) a gene encoding a desired protein having a nonsense mutation or a frame-shift mutation at a desired site.06-09-2011
20110076718IN VIVO INCORPORATION OF AN UNNATURAL AMINO ACID COMPRISING A 1,2-AMINOTHIOL GROUP - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids that comprise a 1,2 aminothiol group into polypeptides. The invention provides translation systems in which polypeptides comprising unnatural amino acids that comprise a 1,2 aminothiol group can be produced. The invention also provides methods for producing polypeptides containing unnatural amino acids that comprise a 1,2 aminothiol group. Also provided by the invention are compositions comprising orthogonal aminoacyl-tRNA synthetases that preferentially aminoacylate a cognate orthogonal tRNA with unnatural amino acids that comprise a 1,2 aminothiol group. The invention provides methods for the synthesis of the unnatural amino acid 2-amino-3-(4-(2-amino-3-mercaptopropan-amido)phenyl)-propanoic acid.03-31-2011
20100159510TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants.06-24-2010
20110262962METHOD FOR PRODUCING PEPTIDES - The present invention provides a method for producing a peptide, comprising culturing a transformant introduced with an expression vector to prepare a culture, and mixing the culture with a carboxy component and an amine component to form the peptide. The expression vector comprises a polynucleotide encoding a protein: (A) having selected deletions in the amino acid sequence of SEQ ID NO:2, (B) having a mutation of one or several amino acid residues in any protein selected from said group (A); (C) having 70% or more amino acid sequence identity to any protein selected from said group (A), (D) encoded by a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to a polynucleotide encoding any protein selected from said group (A), and (E) encoded by a polynucleotide having 70% or more nucleotide sequence identity to the polynucleotide encoding any protein selected from the group (A).10-27-2011
20100028939Use of Galactose Oxidase for Selective Chemical Conjugation of Protractor Molecules to Proteins of Therapeutic Interest - This invention relates to novel compounds, methods for selective chemical conjugation of protractor molecules and the use thereof for diagnostic and/or therapeutic purposes.02-04-2010
20090263859Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from 10-22-2009
20120040397Photo-Crosslinked Nucleic Acid Hydrogels - Methods and compositions are provided for producing hydrogel nucleic acid structures using photo-crosslinking. Methods of using the photo-crosslinked hydrogels for cell-free protein production, and for encapsulating and delivering compounds, are also provided.02-16-2012
20080233611Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as 09-25-2008
20110117597Enhancement of in vitro translation by nanoparticle conjugates - Provided herein are kits and methods suitable for enhancing in vitro translation of a nuclei acid sequence of interest.05-19-2011
20090170153EFFECT OF RADIATION ON CELLULASE ENZYMES - A method for recycling cellulase enzymes. Also provided is a method for producing fermentable carbohydrates, plant leaf protein, and lignin, by adding a cellulase enzyme complex expressed from and on irradiated cellulase complex-producing organisms with sufficient radiation to kill biological activity without destroying all cellulase enzyme complex activity to biomass. The fermentable carbohydrates produced by the method. Also provided are irradiated cellulase-producing organisms for use in converting biomass to fermentable sugars, plant leaf protein, and lignin. A method for producing cellulase enzymes for glucose and other sugar production and protein and lignin extraction by irradiating cellulase-producing organisms, thereby producing the cellulase enzymes is also provided. A system for producing fermentable carbohydrates, plant protein, and lignin, said system comprising irradiated cellulase-producing organisms and biomass is provided.07-02-2009
20080305518O-Linked Glycoforms Of Polypeptides And Method To Manufacture Them - The present invention relates to compositions comprising glycoproteins having altered patterns of O-linked glycosylation, in particular Factor VII, Factor IX, and methods for making these.12-11-2008
20100167340METHOD TO PRODUCE A RECEPTOR CHIP USING BIOTINYLATED PROTEIN - The present invention provides a method for detecting modified LDL, abnormal cells or bacteria using an intermolecular interaction analysis method, in which a region involved in ligand recognition by a receptor is expressed, without modification or as a biotinylated protein, in cells or in a test tube, and thereafter, the expressed region or the expressed biotinylated protein is immobilized via avidin or streptavidin to a solid phase while the orientation thereof is maintained, and the immobilized protein is utilized; and a kit for detecting the modified LDL or the like.07-01-2010
20090042244PROTEIN EXPRESSION YIELD ENHANCEMENT IN CELL-FREE PROTEIN SYNTHESIS SYSTEMS BY ADDITION OF ANTIFOAM AGENTS - Compositions and methods are provided for the in vitro synthesis of biological molecules in reaction mixtures comprising anti-foam agents. The reaction mix comprising antifoam agent may be a scaled up reaction, e.g. in reaction volume greater than at least about 15 ul. Reactions may be performed in various reactors, as known in the art, which include stirred reactors, bubble-column reactors; and the like.02-12-2009
20110070608Method for Producing Corn Gluten Hydrolysate and Corn Gluten Hydrolysate Using the Same - Provided is a method for producing corn gluten hydrolysate comprising: (a) separating corn gluten protein by removing carbohydrate, water soluble sugars, inorganic materials and fiber material; (b) preparing corn gluten protein lysate by carrying out acid hydrolysis, enzymatic hydrolysis or natural fermentation; and (c) increasing a content of branch chain amino acid (BCAA) which is included in the hydrolysate by isolating, concentrating, precipitating, desalting and filtering the resultant corn gluten protein lysate. With improved pre-treatment and concentration processes as compared with the conventional method, the hydrolysate prepared according to the present invention is rich in amino acids and low-molecular-weight peptides. In particular, free amino acids and branched-chain amino acids (BCAA) are included in large quantity.03-24-2011
20120301918METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER - A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.11-29-2012
20080305517Transglutaminase Crosslinked Collagen Biomaterial for Medical Implant Materials - The present invention provides a method for producing an improved biomaterial comprising treating a collagen biomaterial with a transglutaminase under conditions which permit the formation of cross-links within the collagen. Preferably, the transglutaminase is a tissue transglutaminase, a plasma transglutaminase or a microbial transglutaminase. In a preferred embodiment, the collagen biomaterial further comprises a cell adhesion factor, such as fibronectin. The invention further provides biomaterials obtainable by the methods of the invention, and medical implants and wound dressings comprising the same.12-11-2008
20110318779PROCESS FOR PRODUCING RECOMBINANT PROTEIN USING NOVEL FUSION PARTNER - The present invention provides a method of producing polypeptide utilizing a fusion protein of A-B type in the following formula (I), by culturing transformed microorganism comprising DNA sequence encoding the desirable polypeptide; A-B (I). In the above formula (I), A is a fusion partner of 25 or more amino acid residues where aspartic and glutamic acid residues are incorporated to have a net negative charge of 30% or more, and B is the target protein to be produced. The target protein can be isolated from the fusion protein by employing enzymatic cleavage site etc. at the carboxyl-terminus of the fusion partner.12-29-2011
20110318781CYCLODIPEPTIDE SYNTHETASE AND ITS USE FOR SYNTHESIS OF CYCLO(Tyr-Xaa) CYCLODIPEPTIDES - Isolated, natural or synthetic polynucleotide and polypeptide encoded by said polynucleotide, that is involved in the synthesis of cyclodipeptides, recombinant vector comprising said polynucleotide or any substantially homologous polynucleotide, host cell modified with said polynucleotide or said recombinant vector and also methods for in vitro and in vivo synthesizing cyclodipeptides, in particular cyclo(Tyr-Xaa) cyclodipeptides, wherein Xaa is any amino acid and their derivatives and applications thereof.12-29-2011
20100136614Dendrimer-like modular delivery vector - Various nucleic acid-based matrixes are provided, comprising nucleic acid monomers as building blocks, as well as nucleic acids encoding proteins, so as to produce novel biomaterials. The nucleic acids are used to form dendrimers that are useful as supports, vectors, carriers or delivery vehicles for a variety of compounds in biomedical and biotechnological applications. In particular, the macromolecules may be used for the delivery of drugs, genetic material, imaging components or other functional molecule to which they can be conjugated. An additional feature of the macromolecules is their ability to be targeted for certain organs, tumors, or types of tissues. Methods of utilizing such biomaterials include delivery of functional molecules to cells.06-03-2010
20090148887GENETICALLY ENCODED BORONATE AMINO ACID - Provided are compositions comprising an aminoacyl tRNA synthetase that selectively recognizes a boronic amino acid. Methods of incorporating a boronic amino acid into a target polypeptides and target polypeptides produced by the methods are also provided. Methods of producing a protein, which methods comprise site-specifically encoding a boronic amino acid residue into a mutant protein and selectively converting the boronic amino acid residue into a natural amino acid residue are provided. Also provided are compositions comprising a solid phase matrix covalently bound to a polypeptide through a boronic amino acid residue. In addition, compositions comprising a purified population of polypeptide molecules that each comprise a borono amino acid at a selected site are provided.06-11-2009
20100055738Method for Making Acylated Polypeptides - The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ε-amino groups.03-04-2010
20120171720Method of Making Ribosomes - Methods for making in vitro assembled ribosomal subunits and in vitro assembled ribosomes are provided. Methods of transcribing synthetic rRNA and including the synthetic RNA in a synthetic ribosome are provided. Single vessel methods of transcribing synthetic rRNA, forming a synthetic ribosome that includes the synthetic RNA, and allowing the synthetic ribosome to translate a protein are also provided. Methods of screening for novel, synthetic ribosomal subunits and/or ribosomes are provided. Synthetic replicons and methods of making synthetic replicons are also provided.07-05-2012
20120003693NOVEL PEPTIDE-FORMING ENZYME GENE - DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus 01-05-2012
20120003692METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER - A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.01-05-2012
20120003691METHOD OF ISOLATION AND PURIFICATION OF TRYPSIN FROM PRONASE PROTEASE AND USE THEREOF - The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.01-05-2012
20090035814Method for producing circular or multimeric protein species in vivo or in vitro and related methods - A method is disclosed for forming multimeric proteins. The method relies on intermolecular trans-splicing of a split intein either in vivo or in vitro.02-05-2009
20120156721CHEMICALLY MODIFIED MUTANT SERINE HYDROLASES SHOW IMPROVED CATALYTIC ACTIVITY AND CHIRAL SELECTIVITY - This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C06-21-2012
20120156720Compositions and Methods of Producing Enterokinase in Yeast - The present specification disclose polynucleotide molecules encoding an enterokinase, yeast expression constructs including a yeast expression vector and a polynucleotide molecules encoding an enterokinase, yeast cells comprising such a yeast expression construct, methods of producing enterokinase using such yeast cells, and method of cleaving or preparing a recombinant polypeptide using an enterokinase produced by such methods.06-21-2012
20120107870SELECTIVE ENZYMATIC AMIDATION OF C-TERMINAL ESTERS OR ACIDS OF PEPTIDES - The present invention relates to a process for the amidation of C-terminal esters or acids of peptide substrates in solution-phase synthesis of peptides, comprising amidating one or more peptide substrates comprising C-terminal esters or acids using the protease subtilisin in any suitable form in the presence of an ammonium salt derived from an acid having a pKa above 0.05-03-2012
20120156719METHOD FOR UNIVERSAL ENZYMATIC PRODUCTION OF BIOACTIVE PEPTIDES - The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.06-21-2012
20110008827Method to produce recombinant MBP8298 and other polypeptides by nucleotide structure optimization - A method for recombinant production of a polypeptide, exemplified by the therapeutic peptide MBP8298, by recombining and optimizing the nucleic acid encoding said polypeptide, expressing said nucleic acid in a microbial host cell, isolating said polypeptide from the host, and releasing embedded polypeptide from fusion partners or peptide concatamers are explained. Such method may provide increased production or simplified downstream processing for the polypeptide of interest.01-13-2011
20090298119Genetically encoded coumarin amino acids - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as 12-03-2009
20120252065AQUEOUS PROCESS FOR PREPARING PROTEIN ISOLATE AND HYDROLYZED PROTEIN FROM AN OILSEED - The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising: 10-04-2012
20100093025METHOD FOR PRODUCING A YEAST EXTRACT - The present invention relates to a method for producing a yeast extract using an exogenous protease.04-15-2010
20100093024CELL-FREE SYNTHESIS OF PROTEINS CONTAINING UNNATURAL AMINO ACIDS - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of polypeptides containing unnatural amino acids at one or more specified residues of the polypeptide.04-15-2010
20110003332RECOMBINANTLY MODIFIED PLASMIN - Polynucleotides and polypeptides relating to a recombinantly-modified plasmin(ogen) molecule are provided. The plasmin(ogen) molecule has a single kringle domain N-terminal to the activation site present in the native human plasminogen molecule, combined such that no foreign sequences are present, and exhibits lysine-binding and significant enzymatic characteristics associated with the native enzyme.01-06-2011
20090130709Endomannosidases in the modification of glycoproteins in eukaryotes - The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.05-21-2009
20100248303METHOD FOR RECOVERING PEPTIDES/AMINO ACIDS AND OIL/FAT FROM ONE OR MORE PROTEIN-CONTAINING RAW MATERIALS, PRODUCTS PRODUCED BY THE METHOD, AND USE OF THE PRODUCTS - According to a first aspect, hydrolysation of a protein-containing raw material and separation of amino acids/peptides is carried out, wherein the hydrolysation is effected by using the endogenous enzymes of the protein-containing raw material. The hydrolysate is passed through a membrane filter, wherein peptide/amino acids follow a permeate stream, whilst the active enzymes continuously break down any protein residues that are deposited on the membrane surface. The enzymes are passed together with retenate back to the hydrolysis. Furthermore, an amino acid and peptide product and an oil product are described and the use thereof is disclosed.09-30-2010
20120214199LIS-PRO PROINSULIN COMPOSITIONS AND METHODS OF PRODUCING LIS-PRO INSULIN ANALOGS THEREFROM - Lis-Pro modified proinsulin sequences that have a modified C-peptide amino acid and/or nucleic acid modification are presented. Methods for producing Lis-Pro insulin analogs are also disclosed. Highly efficient processes for preparing the Lis-Pro insulin analogs and improved preparations containing the Lis-Pro insulin analogs prepared according to the methods described herein are also provided.08-23-2012
20120129216ENZYMATIC ANTIBODY PROCESSING - The current invention comprises a method for producing an immunoglobulin or immunoglobulin fragment with defined glycostructure comprising the following steps: a) providing an affinity chromatography column eluate containing the immunoglobulin or immunoglobulin fragment, b) incubating the affinity chromatography column eluate with (α1,3)galactosidase of plant origin, e.g. from green coffee beans (EC 3.2.1.22), c) applying the incubated affinity chromatography column eluate to a protein A chromatography material and recovering the immunoglobulin or immunoglobulin fragment from the protein A chromatography material and thereby producing an immunoglobulin or immunoglobulin fragment with defined glycostructure.05-24-2012
20120129215Compositions of Orthogonal Lysyl-tRNA and Aminoacyl-tRNA Synthetase Pairs and Uses Thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.05-24-2012
20120129214PEPTIDE SYNTHESIS USING ENZYMATIC ACTIVATION AN COUPLING - Method for enzymatically synthesising a peptide, comprising enzymatically preparing an ester or a thioester from (i) an N-terminal protected amino acid, an N-terminal protected amino acid C-terminal ester, an N-terminal protected peptide, or an N-terminal protected peptide C-terminal ester and (ii) an alcohol represented by the formula HO—CX05-24-2012
20120231497SELECTIVE ENZYMATIC HYDROLYSIS OF C-TERMINAL TERT-BUTYL ESTERS OF PEPTIDES - The present invention relates to a process for the selective enzymatic hydrolysis of C-terminal esters of peptide substrates in the synthesis of peptides, comprising hydrolysing C-terminal tert-butyl esters using the protease subtilisin. This process is useful in the production of protected or unprotected peptides.09-13-2012
20100209968IMMOBILIZED ENZYMES AND USES THEREOF - The present invention generally relates to uses of immobilized enzymes. Immobilized enzymes can be used for various chemical transformations, separations, and purifications and can be used in sensors and diagnostics08-19-2010
20120135462Polymer-Von Willebrand Factor-Conjugates - The present invention relates to a proteinaceous construct (also designated as polymer-VWF-conjugate) comprising plasmatic and/or recombinant von Willebrand factor (VWF), said VWF being bound to at least one physiologically acceptable polymer molecule, as well as to a complex between said proteinaceous construct and at least one factor VIII (FVIII) protein. The physiologically acceptable polymer molecule can be, for instance, polyethylene glycol (PEG) or polysialic acid (PSA). Further the present invention relates to methods for prolonging the in vivo-half-life of VWF or FVIII in the blood of a mammal having a bleeding disorder associated with functional defects of or deficiencies of at least one of FVIII or VWF.05-31-2012
20120135461PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION COMPRISING THE USE OF AN ALPHA-1,2-MANNOSIDASE - A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of one or more α-1,2-mannosidases from 05-31-2012
20120135460RECOMBINANT PORCINE CHYMOTRYPSIN - The present invention generally relates to the field of proteinases and more specifically to chymotrypsin. In particular, the present invention relates to recombinant porcine chymotrypsin and its use in food applications.05-31-2012
20100173358METHOD FOR OBTAINING A VALUABLE PRODUCT, PARTICULARLY STARCH, FROM GRAIN FLOUR - A process for obtaining a starch and a protein or both from grain flour, the process steps comprising: providing grain flour; mixing the grain flour with processed or fresh water to form a slurry; separating the slurry into it at least two fractions, the at least two fractions including two or more of a heavy A-starch fraction, a protein and B-starch fraction, and a pentosan fraction; and generating a biogas from at least one of the fractions from the separating step, the biogas being used for generating energy.07-08-2010
20120252064Protease Enzyme and Uses Thereof - The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of 10-04-2012
20120214200PROKARYOTIC EXPRESSION CONSTRUCT - A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.08-23-2012
20120214201PROBE INCORPORATION MEDIATED BY ENZYMES - The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants.08-23-2012
20100279343METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER - A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-αβ-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-αβ-diester through a peptide bond.11-04-2010
20120315669METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).12-13-2012
20100291623PROCESS FOR PRODUCING ALPHA-GLYCOSYLATED DIPEPTIDE AND METHOD OF ASSAYING ALPHA-GLYCOSYLATED DIPEPTIDE - The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.11-18-2010
20120258490PROCESS FOR PRODUCING DIPEPTIDES OR DIPEPTIDE DERIVATIVES - The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5′-monophosphate, adenosine-5′-diphosphate and adenosine-5′-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase activity, or a culture of cells having the ability to produce the protein or a treated matter of the culture, and a protein having the activity to ATP-dependently form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture.10-11-2012
20120231496COMPOSITION FOR SYNTHESIZING PROTEIN WITH REDUCED LIPOPOLYSACCHARIDE CONTAMINATION, METHOD FOR PRODUCING PROTEIN USING SAID COMPOSITION - According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.09-13-2012
20120231498PROCESSING ENZYMES FUSED TO BASIC PROTEIN TAGS - The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.09-13-2012
20110039300ANTIBODIES WITH ENHANCED ADCC FUNCTIONS - The present invention concerns antibodies with enhanced antibody-dependent cell mediated cytotoxicity (ADCC) and method for preparation thereof.02-17-2011
20120322101PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION - A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more α-1,2-mannosidases.12-20-2012
20120322100METHODS FOR PRODUCING MODIFIED GLYCOPROTEINS - Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins I humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans such as Man12-20-2012
20110045530ENZYMATIC CONJUGATION OF BIOACTIVE MOIETIES - The present invention relates to a method for selective conjugation of bioactive moieties to a polymer or polymerisable compound. The method is more specifically related to the selective conjugation of bioactive moieties to a pendant carboxylic acid, ester or thioester group in which the pendant group is part of a polymer or a polymerisable compound, wherein the method comprises contacting the polymer or polymerisable compound with a hydrolytic enzyme to catalyse the conjugation between the bioactive moiety and the pendant carboxylic acid, ester or thioester group. The conjugation of the bioactive moieties may occur prior to, during or after polymerization of the polymerisable compound. The conjugation of the bioactive moieties may also occur after the polymer is given a form.02-24-2011
20100203583Alteration and modulation of protein activity by varying post-translational modification - Embodiments of the invention include methods of altering the enzymatic activity or solubility of an extremophilic enzyme or post-translationally modifying a protein of interest via using isolated or partially purified glycosyltransferases and/or post-translational modification proteins, extracts of cells comprising glycosyltransferases and/or post-translational modification proteins, and/or in cells comprising one or more glycosyltransferases and/or post-translational modification proteins.08-12-2010
20100203582Protein Expression System Involving Mutated Severe Respiratory Syndrome-Associated Coronavirus 3C-Like Protease - A mutated severe acute respiratory syndrome-associated coronavirus 3C-like protease and use thereof for cleaving a protein that includes a cleavage site recognizable by the mutated protease to yield a polypeptide fragment of interest.08-12-2010
20110236926Host Cells and Methods Of Producing Disulfide Bond Containing Proteins - The invention provides for genetically modified 09-29-2011
20100233757Synthesis and Use of Anti-Reverse Phosphorothioate Analogs of the Messenger RNA Cap - New RNA cap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at the 2′-O position of 7-methylguanosine that prevent them from being incorporated in the reverse orientation during in vitro synthesis of mRNA and that hence are “anti-reverse cap analogs” (ARCAs). The ARCA modification ensures that the S atom is precisely positioned within the active sites of cap-binding proteins in both the translational and decapping machinery. The new S-ARCA analogs are resistant to in vivo decapping enzymes. Some S-ARCAs have a higher affinity for eIF4E than the corresponding analogs not containing a phosphorothioate group. When mRNAs containing the various S-ARCAs are introduced into cultured cells, some are translated as much as five-fold more efficiently than mRNAs synthesized with the conventional analog m09-16-2010
20110262963BIOSYNTHETICALLY GENERATED PYRROLINE-CARBOXY-LYSINE AND SITE SPECIFIC PROTEIN MODIFICATIONS VIA CHEMICAL DERIVATIZATION OF PYRROLINE-CARBOXY-LYSINE AND PYRROLYSINE RESIDUES - Disclosed herein is pyrroline-carboxy-lysine (PCL), a pyrrolysine analogue, which is a natural, biosynthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein.10-27-2011
20100216185ENGINEERED VERSIONS OF POLYSIALYLTRANSFERASES WITH ENHANCED ENZYMATIC PROPERTIES - The invention relates to poly-sialyltransferse polypeptides with enhanced solubility and activity and methods of using the poly-sialyltransferases for production of poly-sialylated end products, e.g., oligosaccharides, glycoproteins and glycolipids.08-26-2010
20120288892Methods for Producing Polypeptidies in Protease-Deficient Mutants of Trichoderma - The present invention relates to mutants of a parent 11-15-2012
20100184135MONO CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO PROTEIN SYNTHESIS SYSTEM - This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.07-22-2010
20130011873ENZYME THAT CATALYZES A PEPTIDE-FORMING REACTION FROM A CARBOXY COMPONENT AND AN AMINE COMPONENT, MICROBE PRODUCING THE SAME, AND A METHOD OF PRODUCING A DIPEPTIDE USING THE ENZYME OR MICROBE - DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus 01-10-2013
20110159538Process for Preparation of Insulin Compounds - The present invention relates to the preparation of insulin compounds including their analogs or derivatives thereof from their corresponding precursor forms by a one step enzymatic reaction involving the combinatorial and concurrent use of optimal quantities of trypsin and carboxypeptidase B that work synergistically directing the reaction in a controlled manner to avoid production of random undesired byproducts. Particularly, the enzymatic conversion reactions of the instant invention offer advantages of reduction in the number operational steps, higher yield and purity of the desired end products.06-30-2011
20080241879Microorganism and protease decomposing proteins recalcitrant to proteolysis - The invention provides novel biologically pure cultures of microorganisms high in protease activity and capable of decomposing proteins recalcitrant to proteolysis as contained in garbage, waste water, organic waste liquids, industrial wastes and the like, a protease produced by such microorganisms and capable of decomposing proteins recalcitrant to proteolysis, and a method of utilizing the same. The novel culture is of a soil-derived microorganism belonging to 10-02-2008
20080241878Method For Enzmatic Cross-Linking Of A Protein, Cross-Linked Protein Thus Obatained And Use Thereof - The present invention provides methods for enzymatic cross-linking of a protein comprising performing a reaction for enzymatic cross-linking of said protein in the presence of a phenolic saccharide, wherein said reaction comprises the step of forming protein-protein cross-links. The present invention further provides a cross-linked protein obtainable by a method according to the invention as well as a protein that is cross-linked by a phenolic saccharide and the use of such proteins in a foodstuff. The invention also provides a foodstuff comprising a cross-linked protein of the invention as well as the use of a phenolic saccharide as a cross-linking agent for proteins.10-02-2008
20080220470PROTEIN HYDROLYSATE RICH IN TRIPEPTIDES - The present invention describes a protein hydrolysate which is rich in tripeptides whereby the tripeptides are rich in proline at one end of the peptide.09-11-2008
20130177940C-TERMINAL MODIFICATION OF POLYPEPTIDES - The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaa07-11-2013
20110269182Method for Selectively Modifying a Protein - The present invention relates generally to a novel method of introducing property modifying groups to a protein. In particular, the present invention relates to the derivatization of lysine residues, as well as new conjugates of growth hormones with improved pharmacological properties, and methods for their preparation and use in therapy.11-03-2011
20130115655METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES - The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.05-09-2013
20130102029Processing Biomass - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.04-25-2013
20130143262METHOD FOR PRODUCING PEPTIDE - Peptides may be produced by allowing (A) a first amino acid or peptide, which is converted into its ionic liquid form, (B) a second amino acid or peptide, and (C) a peptide hydrolase to simultaneously exist in a single reaction system, wherein the first amino acid or peptide, which is converted into its ionic liquid form, is used as both a reaction solvent and a reaction starting material; and forming a peptide bond between the first amino acid or peptide and the second amino acid or peptide. By such a process, it is possible to synthesize a peptide at a high concentration and at a high yield, and the method is excellent for producing peptides on an industrial scale.06-06-2013
20130143263METHOD AND APPARATUS FOR CONVERSION OF CELLULOSIC MATERIAL TO ETHANOL - The present invention provides an apparatus and a method for conversion of cellulosic material, such as chopped straw and corn stover, and household waste, to ethanol and other products. The cellulosic material is subjected to continuous hydrothermal pre-treatment without addition of chemicals, and a liquid and a fibre fraction are produced. The fibre fraction is subjected to enzymatic liquefaction and saccharification. The method of the present invention comprises: 06-06-2013
20110217732METHOD AND COMPOSITIONS FOR THE DETECTION OF PROTEIN GLYCOSYLATION - The invention provides methods and compositions for the rapid and sensitive detection of post-translationally modified proteins, and particularly of those with post-translational glycosylations. The methods can be used to detect O-GlcNAc posttranslational modifications on proteins on which such modifications were undetectable using other techniques. In one embodiment, the method exploits the ability of an engineered mutant of β-1,4-galactosyltransferase to selectively transfer an unnatural ketone functionality onto O-GlcNAc glycosylated proteins. Once transferred, the ketone moiety serves as a versatile handle for the attachment of biotin, thereby enabling detection of the modified protein. The approach permits the rapid visualization of proteins that are at the limits of detection using traditional methods. Further, the preferred embodiments can be used for detection of certain disease states, such as cancer, Alzheimer's disease, neurodegeneration, cardiovascular disease, and diabetes.09-08-2011
20110223633METHODS AND REAGENTS FOR ENRICHMENT AND CHARACTERIZATION OF PHOSPHORYLATED BIOMOLECULES - An affinity matrix comprising a metal ion covalently attached thereto and methods for making and using the same are described. The matrix has affinity for various phosphorylated biomolecules, such as phosphoproteins/phosphopeptides. The matrix may be used in a variety of different applications, including phospho-biomolecule (e.g., phosphoprotein and phosphopeptides) enrichment/purification and characterization applications. Also provided are kits and systems that include the matrix.09-15-2011
20130137139PROCESSES FOR PREPARING TUBULYSINS - Tubulysins are a series of naturally occurring cytotoxic agents that are of interest as anticancer therapeutic agents. Processes and intermediates useful for preparing naturally occurring and non-naturally occurring tubulysins and analogs and derivatives thereof are described.05-30-2013
20130149740METHOD FOR THE PRODUCTION OF A POLYMERIZED PRODUCT - The invention discloses a method for the production of a polymerized product comprising the following steps: 06-13-2013
20110275120Fusion Proteins With Cleavable Spacers and Uses Thereof - A polypeptide comprising a first protein domain, a second protein domain, and a dithiocyclopeptide spacer containing at least one protease cleavage site, wherein the dithiocyclopeptide is exogenous relative to the first or second protein domain, and wherein the first and second protein domains are operably linked by the dithiocyclopeptide. Also disclosed are methods of producing the polypeptide and delivering the protein domains into a cell.11-10-2011
20110275119METHODS FOR RIBOSOMAL SYNTHESIS OF POLYPEPTIDES CONTAINING UNNATURAL N-TERMINAL GROUPS AND APPLICATIONS THEREOF - The present invention aims to synthesize a polypeptide having an unnatural structure at the N-terminus via a biosynthetic process by translation of amino acid sequence information encoded by a nucleic acid. A polypeptide having any amino acid at the N-terminus is synthesized by using an ARS ribozyme that catalyzes the acylation of tRNA with any amino acid to attach any amino acid to an initiator tRNA, thereby initiating a translation with the initiator tRNA.11-10-2011
20100075375METHODS FOR THE PURIFICATION OF POLYPEPTIDE CONJUGATES - The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.03-25-2010
20120282652PREPARATION OF PEPTIDE MIXTURES BY PROTEASE CATALYSIS DESIGNED TO PROVIDE USEFUL BIOLOGICAL AND PHYSICAL PROPERTIES - A process for preparing unique peptide mixtures with a broad range of uses using combinations of natural and non-natural amino acid alkyl ester monomers and specific combinations of these monomers as dimers, trimers and higher oligomers selected from the large group of structural motifs that lead to useful physical and/or biological properties and that have been or may be identified from solid state peptide synthesis, isolation of peptides from natural sources, and production of peptides by recombinant DNA methods, or identification of peptides by recombinant methods such as phage display, the method of peptide synthesis comprising a) admixing one or more natural and non-natural amino acid alkyl ester monomer, dimer, trimer and higher oligomers with one or more proteases in a reaction medium; b) heating the mixture to between about 5° C. to about 90° C. for between 5 minutes and 24 hours; and c) recovering the formed oligopeptide.11-08-2012
20130183712METHOD AND COMPOSITIONS FOR THE DETECTION OF PROTEIN GLYCOSYLATION - The invention provides methods and compositions for the rapid and sensitive detection of post-translationally modified proteins, and particularly of those with posttranslational glycosylations. The methods can be used to detect O-GlcNAc posttranslational modifications on proteins on which such modifications were undetectable using other techniques. In one embodiment, the method exploits the ability of an engine˜red mutant of β-1,4-galactosyltransferase to selectively transfer an unnatural ketone functionality onto O-GlcNAc glycosylated proteins. Once transferred, the ketone moiety serves as a versatile handle for the attachment of biotin, thereby enabling detection of the modified protein. The approach permits the rapid visualization of proteins that are at the limits of detection using traditional methods. Further, the preferred embodiments can be used for detection of certain disease states, such as cancer, Alzheimer's disease, neurodegeneration, cardiovascular disease, and diabetes.07-18-2013
20110312027Production of Carrier-Peptide Conjugates Using Chemically Reactive Unnatural Amino Acids - Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.12-22-2011
20130189732Protease Variants - The invention relates to a novel 3D structure encoding a 07-25-2013
20120021458THERMOTOLERANT TRANSGLUTAMINASE ORIGINATING IN ACTINOMYCES - The present invention provides transglutaminases with improved heat resistance. Specifically, the present invention provides mutant transglutaminase proteins with improved heat resistance as obtained by introducing appropriate mutations into transglutaminases, which results in the incorporation of a disulfide bond.01-26-2012
20120021457PROTEIN CONCENTRATES AND ISOLATES, AND PROCESSES FOR THE PRODUCTION THEREOF FROM MACROALGAE AND/OR MICROALGAE - Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from macroalgae and/or microalgae using low g-force centrifugation.01-26-2012
20130196372DEVICE AND METHOD FOR CELL FREE ANALYTICAL AND PREPARATIVE PROTEIN SYNTHESIS - A device is disclosed which contains one or more pores 08-01-2013
20120040398ENGINEERED CLEAVAGE HALF-DOMAINS - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.02-16-2012
20130203110SYSTEM FOR THE IN VITRO TRANSCRIPTION AND TRANSLATION OF MEMBRANE PROTEINS - System for the in vitro transcription and translation of membrane proteins comprising i) a micro-fluidic chip having at least one micro-fluidic reaction chamber and micro-fluidic channels to allow fluid to flow through the chip and into and from the at least one reaction chamber, ii) the at least one micro-fluidic reaction chamber being provided with at least one electrode base plate of conductive or semi-conductive material, and iii) lipid vesicles or a lipid membrane being bound or tethered to the at least one electrode base plate either directly or through spacer molecules.08-08-2013
20130203111Aldehyde Tags, Uses Thereof in Site-Specific Protein Modification - The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.08-08-2013
20130203112Site-Specific Incorporation of Phosphoserine Into Proteins in Escherichia Coli - Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNA08-08-2013
20120077224 UNNATURAL AMINO ACID INCORPORATION IN EUKARYOTIC CELLS - This disclosure concerns compositions and methods for improving the incorporation of unnatural amino acids (UAAs) into proteins in eukaryotic cells. It is shown herein that mutation of a prokaryotic tRNA synthetase to increase the interaction with the corresponding tRNA anticodon region results in increased UAA incorporation efficiency in mammalian cells.03-29-2012
20120077223Method for single oxygen atom incorporation into peptides - Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 03-29-2012
20130095523NOVEL FUNGAL STRAIN BEAUVERIA SP. MTCC 5184 AND A PROCESS FOR THE PREPARATION OF ENZYMES THEREFROM - A fungal strain 04-18-2013
20120088269SOLUTION FOR CELL-FREE PROTEIN SYNTHESIS, KIT FOR CELL-FREE PROTEIN SYNTHESIS, AND METHOD OF PROTEIN SYNTHESIS - This invention enables synthesis of proteins that were difficult to synthesize via a conventional cell-free protein synthesis system and increases the amount of proteins synthesized. Cell-free protein synthesis is carried out in a solution for cell-free protein synthesis containing a certain compound, such as trimethylglycine, L-carnitine, or sarcosine.04-12-2012
20120088268RECOMBINANT PRODUCTION OF PEPTIDES - The present invention relates to repetitive self-assembling precursor proteins, nucleic acid sequences and expression constructs encoding the same, and to methods for recombinant production of peptides using such precursor proteins.04-12-2012
20130210073METHODS OF INCORPORATING AMINO ACID ANALOGS INTO PROTEINS - The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.08-15-2013

Patent applications in class Enzymatic production of a protein or polypeptide (e.g., enzymatic hydrolysis, etc.)