Entries |
Document | Title | Date |
20080206807 | Methods, Reagents, Devices and Instrumentation For Preparing Impregnated Tissue Samples Suitable For Histopathological and Molecular Studies - A process for the production of paraffin sections of biological tissue, especially for molecular pathology studies is disclosed. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. A kit and a processing station for automating paraffin embedding of a tissue sample suitable for histopathological and molecular analysis is also described. A bio-indicator system is described for measuring the degree of crosslinking. A tissue sample holding means or a vial which includes a tissue sample holding means provided with a data logging device capable of registering and transmitting data regarding the sample and conditions where the sample was processed is also disclosed. | 08-28-2008 |
20080213824 | Synthesis of Labeled Probes - Improvements in FISH staining employing an aqueous acetonitrile/dimethylsulfoxide mixture in a fluorophore coupling reaction. | 09-04-2008 |
20080241876 | Information notification sample processing system and methods of biological slide processing - A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing. | 10-02-2008 |
20080268495 | Preparing Biological Samples for Analysis - Methods and devices for preparing a biological sample for analysis are described. The biological sample from an organism has at least macromolecule having a primary structure that naturally degrades after the sample is removed from the organism. The method includes causing the biological sample to adopt a shape to permit rapid and uniform heating. The shaped sample is then rapidly and uniformly heated, thereby altering a secondary structure of the macromolecule while preserving its primary structure. | 10-30-2008 |
20080268496 | Composition for the preparation of histological, autopsical, cytological samples - Composition based on alkyl C | 10-30-2008 |
20080299605 | USEFUL SPECIMEN TRANSPORT APPARATUS WITH INTEGRAL CAPABILITY TO ALLOW THREE DIMENSIONAL X-RAY IMAGES - The present invention provides new and useful features and mechanisms for the localization and transport of biopsy specimens. The invention having a specimen board, an absorbent material in operative engagement and in coplanar alignment with the specimen board, a compression sheet, radio opaque indicia located within the specimen board, and a flexible connection between the compression sheet and the specimen board, and an attachment device which provides for removable engagement of the specimen board and compression sheet. The apparatus further provides for a clear visualization window and operating instructions. The absorbent material is capable of adjustable movement between a first and second position, providing orthogonal positioning relative to the specimen board. As a result, the apparatus may be used to create three dimensional radiographic images allowing tissue analysis resulting in orthogonal views while maintaining original positional reference points. | 12-04-2008 |
20090004691 | Enhanced Fluidic Method and Apparatus for Automated Rapid Immunohistochemistry - A sample processing system that may be configured to achieve rapid sample processing such as rapid histochemical processing may involve a wave element that can use angular microscopic slide movements to cause repeated elimination and reapplication of a fluidic substance perhaps through the action of capillary motion in order to refresh a microenvironment adjacent to a sample such as a biopsy or other such sample. Through such refreshing of a microenvironment, depletion of the microenvironment is avoided and the time necessary for slide processing may be dramatically shortened from a more common 60 to 120 minute to perhaps less than 15 minutes so as to permit use of such a system in an intraoperative or surgical environment such as recommended by the College of American Pathologists intraoperative guidelines or the like. Patients may thus avoid a need to be subjected to an additional surgical procedure when lab results become available to see if tumors or the like were fully removed in a prior procedure. | 01-01-2009 |
20090035809 | Modified Carbocyanine Dyes and Their Conjugates - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens. | 02-05-2009 |
20090035810 | MODIFIED CARBOCYANINE DYES AND THEIR CONJUGATES - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens. | 02-05-2009 |
20090042242 | TWO-STEP GRAM STAINING METHOD - The present invention provides a two-step Gram staining method. The method is conducted by using a clean slide, followed by adding 10˜30 μl primary stain from a reagent kit, adding an equivalent amount of specimens uniformly on said slide without drying and frame fixing, then immediately adding 30˜60 μl counter-stain on said slide for incubating 15 seconds, rinsing with water, and finally examining under a microscope, the result appears color contrast on the center of the slide between the Gram-negative bacteria performing light red color and the Gram-positive bacteria performing deep purple color. | 02-12-2009 |
20090081722 | SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 03-26-2009 |
20090104654 | LOW VOLATILITY HIGH TEMPERATURE TISSUE CONDITIONING CROSS-REFERENCE TO RELATED APPLICATION - Solutions exhibiting little or no evaporative loss at elevated temperatures, i.e., in excess of 100° C., are employed in place of conventional aqueous-based antigen retrieval solutions. | 04-23-2009 |
20090117610 | Methods and Compositions for Identifying a Cell Phenotype - A method of staining or pre-staining at least one cell is provided. The method comprising contacting the at least one cell with a staining agent selected from the group consisting of an extract of a | 05-07-2009 |
20090117611 | DEVICE AND METHOD FOR WETTING OBJECTS - The invention relates to a device and a method for wetting objects with a liquid by means of a system for carrying a specimen slide that is disposed at a distance from a platform. To reduce liquid consumption, the specimen slide is raised or lowered relative to the platform by means of a system. | 05-07-2009 |
20090136992 | SIMPLIFIED TISSUE PROCESSING - Improved systems and methods for tissue processing are described here. The chemical process and the construction of the apparatus are simplified by using only two different solutions in two separate reaction modules. They are compatible with processing of tissue specimens for genetic analysis, histology, in situ antibody binding and hybridization, archival preservation of morphology and nucleic acids, and combinations thereof. | 05-28-2009 |
20090142797 | BLOOD DILUENT AND METHOD OF USE THEREOF - Blood diluent for use in the analysis of blood components may include at least one purine compound or salt thereof. The blood diluent may also include at least one of alkali metal salt and/or at least one organic buffers and/or inorganic buffer. The blood diluent may be utilized to stabilize blood cells during storage and facilitate the analysis thereof. | 06-04-2009 |
20090142798 | METHOD OF SELECTIVELY LYSING NON-VIABLE CELLS IN CELL POPULATION IN SAMPLE - The present invention provides a method of selectively lysing non-viable cells from a cell population within a sample, the method including selectively lysing non-viable cells by mixing a cell-containing sample with a lysis buffer including a non-ionic surfactant and a divalent cation salt. | 06-04-2009 |
20090176270 | ASYMMETRIC CYANINE FLUORESCENT DYES, COMPOSITIONS AND THEIR USE IN STAINING BIOLOGICAL SAMPLES - Asymmetric cyanine fluorescent dyes are represented by general formula I. These kinds of dyes may be used as a staining agent for nucleic acids, with the spectra at 600-900 nm in the near-infrared region and without interference from background fluorescence. These kinds of dyes may be useful with small-type red semiconductor lasers as the light source (such as 633 nm). Compositions comprising these dyes and methods for staining biological samples using these dyes or compositions are also provided. | 07-09-2009 |
20090176271 | Systems for Efficient Staining and Sorting of Populations of Cells - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 07-09-2009 |
20090253163 | ITERATIVE STAINING OF BIOLOGICAL SAMPLES - Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell. | 10-08-2009 |
20090325222 | Tissue Sample Preparation and MALDI MS Imaging Thereof - Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis. | 12-31-2009 |
20090325223 | MAGNETIC IMMUNOHISTOCHEMICAL STAINING DEVICE AND METHODS OF USE - The subject invention concerns a device for use in staining samples or tissues of interests on slides. The subject invention also concerns methods of using the device to stain a specimen on a slide or to detect antibody and proteins of interest of a tissue section. | 12-31-2009 |
20100003716 | ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE - The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers. | 01-07-2010 |
20100047859 | SILICA-BASED FLUORESCENT NANOPARTICLES - A composition including the reaction product of: an organic silane of Formula SiR | 02-25-2010 |
20100068757 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated microscope slide staining system and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments or a pressurizable common chamber for individually and independently processing a plurality of microscope slides. The apparatus preferably features independently movable slide support elements each having an individually operable heating element. | 03-18-2010 |
20100075372 | METHOD FOR DEPARAFFINIZATION OF PARAFFIN-EMBEDDED SPECIMEN AND METHOD FOR ANALYSIS OF PARAFFIN-EMBEDDED SPECIMEN - The present invention provides a method for deparaffinization useful for mass spectrometric imaging from a paraffin-embedded specimen. A method for deparaffinization of paraffin-embedded specimen comprising the steps of: exposing a biological sample by subjecting a specimen in which the biological sample is embedded in paraffin, to an organic solvent that is compatible with the paraffin under heating condition, to make the paraffin be melted and dissolved in the organic solvent; and removing the paraffin from the specimen by separating the organic solvent dissolving the paraffin, from the exposed biological sample. | 03-25-2010 |
20100075373 | Multi-Spectral Imaging Including At Least One Common Stain - A method including: providing a sample with M components to be labeled, where M>2; labeling the components with N stains, where N03-25-2010 | |
20100093021 | Hardening of ordered films of silica colloids - Sintering self-assemblies of calcined colloidal silica particles results in sintered colloidal crystals that are free of cracks that can be resolved using optical microscopy. The sintered colloidal crystals have significantly improved strength and durability, and withstand aggressive handling. Surface rehydroxylation of the sintered colloidal crystals enables subsequent chemical modification. | 04-15-2010 |
20100105104 | CHIP FOR SAMPLING CELL COMPONENT, SYSTEM FOR ANALYZING CELL COMPONENT AND METHOD OF ANALYZING CELL COMPONENT USING THE SAME - The present invention provides a method, a chip device, and a system for quantitatively measuring intercellularly and intracellularly-localized mRNA and protein without loss of local space information. In the present invention, cell content is trapped atop a substrate or an electrode in the form of a two-dimensional projection of a cell. Accordingly, electrophoretic force is used, or the cellular moisture content is instantaneously evaporated and immobilized. mRNA immobilized to a two-dimensional surface is identified with a labeled probe which hybridizes to the mRNA. | 04-29-2010 |
20100105105 | USE OF A REFERENCE SOURCE WITH ADAPTIVE OPTICS IN BIOLOGICAL MICROSCOPY - Methods of microscopic imaging of biological tissue using adaptive optics technology to improve the image focus and sharpness. Wavefront measurements are taken by using a novel method of seeding biological tissue by using a fluorescent microsphere as a “guide star” as a natural point-source reference. The current methods are capable of improving the Strehl ratio of modern biological microscopes as much as 15 times. | 04-29-2010 |
20100136612 | HORIZONTAL ANTIGEN RETRIEVAL - The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation. | 06-03-2010 |
20100151511 | Biological culture assembly - The invention relates to a resealable or a single use cell culture assembly for use in growing, storing and transporting, cell and tissue cultures. The assembly includes a support frame for receiving a base member insert having an upper surface, such as a microscope slide, and a well frame, preferably partitioned into a plurality of well compartments having sidewalls with upper and lower surfaces, upper and lower edges, and upper and lower well openings. The well frame is adapted to be operatively positioned on the upper surface of the base member, and includes a sealing means positioned on or within the lower edge of the well frame. The sealing means is adapted to operatively create a liquid-impermeable releasable seal or barrier when the lower surface of the well frame is positioned on the upper surface of the base member, such that the sealing means is releasably positioned on the upper surface of the base member for maintaining a liquid-impermeable barrier between well compartments. The assembly may include a cover or lid positioned over the upper opening of the well frame. When the assembly is in a closed position, the well frame can be secured to the support frame by a variety of closure means. | 06-17-2010 |
20100151512 | Haemolysator - A haemolysator having a sonotrode plate and oscillation generating elements acting thereupon, wherein the oscillation generating elements are set into mechanical oscillations by an electrical AC-signal generator. The haemolysater also includes a sample chamber to which the sonotrode plate transmits mechanical oscillations, and has oscillation generating elements that are excitable toward mechanical oscillations in a wide frequency band. | 06-17-2010 |
20100159507 | SHREDDER FOR MECHANICAL DISRUPTION BY GENTLE CONTROLLED COMPRESSIVE ROTATION - The systems and techniques of the present invention can also synergistically utilize mechanical disruption processes with the use of high hydrostatic pressure extraction, such as pressure cycling extraction techniques to achieve high yield of difficult to extract sample constituents without generating high shear stress or high temperatures. | 06-24-2010 |
20100173353 | Lysis Reagent Formulation - Subject matter of the invention is a lysis reagent formulation for binding components of a sample in the form of a tablet comprising a multitude of magnetic particles which are held together with the aid of excipients, a chaotropic salt and an excipient and the use of this lysis reagent formulation in analytical tests. | 07-08-2010 |
20100178667 | PERIODIC ACID-SCHIFF STAINING WITH DETECTION IN THE INFRARED RANGE - Provided is a method of detecting the presence and quantitating the amount of glycogen from a biological sample. This method employs PAS staining with detection in the infrared range. | 07-15-2010 |
20100184125 | BIOMARKERS AND METHODS FOR DETERMINING SENSITIVITY TO INSULIN GROWTH FACTOR-1 RECEPTOR MODULATORS - IGF1R biomarkers useful in a method for identifying and monitoring a mammal that will respond therapeutically to a method of treating cancer comprising administering an IGF1R modulator, wherein the method comprises (a) exposing the mammal to the IGF1R modulator and (b) measuring in the mammal the level of the at least one biomarker, wherein a difference in the level of the at least one biomarker measured in (b) compared to the level of the biomarker in a mammal that has not been exposed to the IGF1R modulator indicates that the mammal will respond therapeutically to the method of treating cancer and (c) wherein the level of the biomarker in a mammal after exposure to a IGF1R modulator indicates that the mammal has responded therapeutically to the method of treating cancer. | 07-22-2010 |
20100184126 | SAMPLING DEVICE - A portable sampling device ( | 07-22-2010 |
20100248301 | Tissue Embedding Apparatus, And Method For Operating A Tissue Embedding Apparatus - The present invention relates to a tissue embedding apparatus ( | 09-30-2010 |
20100255531 | ANALYSIS OF SINGLE BIOLOGICAL CELLS - An analysis of type, state or other distinguishing features of individual cells from body fluids, smears or tissues includes the steps of depositing the cells, with a minimum possible overlap, on a mass spectrometric sample support, determining the coordinates of the cells, coating the sample support with a layer of small crystals of a matrix substance, positioning the cells, inside a mass spectrometer, according to their known coordinates with a movement device into the position of the laser focus, acquiring mass spectra of the individual cells with ionization of the cell components by matrix assisted laser desorption, and using the mass spectra for an analysis of type, state or other distinguishing features of the cells. | 10-07-2010 |
20100255532 | Automated Molecular Pathology Apparatus Having Fixed Slide Platforms - Apparatus and methods for automatically staining or treating multiple tissue samples mounted on slides are provided, in which the slides and reagent bottles are held in fixed position, and the reagent, wash and coverslipping solutions brought to the slides. Alternatively, the slides are held in fixed position, while the reagent, wash and coverslipping solutions brought to the slides. | 10-07-2010 |
20100267081 | METHOD AND DEVICE FOR FIXING/STABILISING A SAMPLE - The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container with a maximum overall height of 10 mm, preferably of 5 mm, and the container filled with the sample is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized. | 10-21-2010 |
20100279340 | Imaging agents for protein misfolding - Charged and neutral small fluorescent molecules based upon the styryl scaffold are useful as imaging agents for misfolded proteins such as amyloid plaque. Charged molecules are prepared using pyrrolidine catalyzed reactions by solution-phase synthesis. Neutral styryl molecules are prepared using acetic anhydride catalyzed reactions, Horner-Emmons reactions or Wittig reactions. | 11-04-2010 |
20100279341 | METHODS AND SYSTEM FOR ANALYZING CELLS - The invention relates to a method for analyzing cells that are present as closed clusters. According to said method, a planar tissue preparation is subjected to an identification staining of the cell nuclei and a target structure staining of cell objects that is different from the identification staining. Digital images are recorded of the stained tissue preparation by means of an electronic image recording device and at least one image of a subsection of the tissue cut is displayed in at least one coloration. According to the inventive method, at least one parameter of the cell nuclei and at least one parameter of the cell objects labeled by target structure staining is restricted to a predetermined range of values. Cell nuclei and cell objects whose parameters correspond to the respective parameter range(s) are detected and optionally displayed using image processing algorithms in the image of said subsection. The image content of at least one image detected for the cell nuclei is correlated with the image content of at least one image detected for the target-structure stained cell objects to detect the individual cells. On the basis of the cell nuclei identified a cell growth or a cell enlargement is induced using a predetermined arithmetic algorithm to reconstruct the individual cells. In doing so it is made sure that neighboring cells do not fuse. The number of reconstructed individual cells is determined and/or the individual cells are divided into populations according to certain parameters. | 11-04-2010 |
20100323395 | Method For Automatically Processing Tissue Samples In A Tissue Processor - In the automatic processing of tissue samples in a tissue processor, the tissue samples are placed in a retort of the tissue processor. The tissue samples are exposed to a fixation reagent (FIX) in the retort. Afterwards, the tissue samples are exposed to a dehydrating reagent (ALK). Thereafter, the retort is cleared with an intermedium (INT) for removing the dehydrating reagent (ALK). Finally, the tissue samples are treated with a carrier material (CAR). Between the clearing of the retort with the intermedium (INT) and the treatment of the tissue samples with the carrier material (CAR), the retort is cleared with a carrier material protecting reagent (CARPRO) in which the intermedium (INT) and the carrier material (CAR) can be mixed. | 12-23-2010 |
20100323396 | PLATELET MEASUREMENT REAGENT, PLATELET MEASUREMENT REAGENT KIT, AND PLATELET MEASUREMENT METHOD - A reagent for measuring platelets comprising Nile Blue hydrogensulfate, or Nile Blue and an acid, a reagent kit for measuring platelets comprising the reagent for measuring platelets, and a method for measuring platelets using the reagent or reagent kit. | 12-23-2010 |
20110014647 | System and Method for Analyzing Samples Labeled with 5, 10, 15, 20 Tetrakis (4-Carboxyphenyl) Porphine (TCPP) - One embodiment of the present invention provides for a method of determining if a sputum sample contains dysplastic or carcinomic cells by obtaining a sputum sample containing cells. The sputum sample is labeled with TCPP to stain cells suspected to be dysplastic or carcinomic. The labeled sputum sample is excited with an excitation wavelength of light of about 475 nm+/−30 nm and emission at about 560 nm+/−30 nm is detected from cells identified to be macrophages. An imager focuses on the plasma membrane of one or more cells suspected to be dysplastic or carcinomic and emission at about 655 nm+/−30 nm, if present, is detected for TCPP labeled cells of the sputum sample after focusing on the plasma membrane of the cells of the sputum sample. Photon flux for each pixel of a sensor is measured to obtain a value for the imaged cell. The measured value is scored to determine if a cell is cancerous or dysplastic. | 01-20-2011 |
20110104747 | Method of Concentrating Beads in a Droplet - Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume. | 05-05-2011 |
20110124040 | FIXATIVE OF POLYMERIZED CARBON NANOTUBES ENCAPSULATING OSMIUM NANOPARTICLES FOR BIOLOGICAL TISSUE - A fixative for biological tissue made up of polymerized carbon nanotubes encapsulating osmium nanoparticles and its method of synthesis are disclosed. Carbon nanotubes are first oxidized. Next, the oxidized carbon nanotubes and monohydrated citric acid are mixed to synthesize carbon nanotubes grafted with poly(citric acid). The carbon nanotubes grafted with poly(citric acid) are then mixed with an osmium source to synthesize carbon nanotubes grafted with poly(citric acid) encapsulating osmium nanoparticles. The nano-fixative of this application has been shown to improve fixation of biological tissue relative to well-known fixatives. | 05-26-2011 |
20110143392 | Biological fixative and method of using the biological fixative - A composition that includes an aldehyde, alcohol, and a ketone, the volumetric ratio of the alcohol to the ketone in the composition ranging from as low as about 0.8:1 to as high as about 4.5:1 and the volumetric ratio of the alcohol to the aldehyde in the composition ranging from as low as about 41.5:1 to as high as about 450:1. | 06-16-2011 |
20110143393 | AUTOMATIC DEVICE FOR CARRYING OUT DETECTION REACTIONS, AND METHOD FOR DOSING REAGENTS ONTO MICROSCOPE SLIDES - The invention relates to an automatic device to meter reagents onto a multitude of slides comprising holding devices for a multitude of slides which contain each a multitude of separated slots which are adapted to absorb cell or tissue samples; a dosing head unit with a multitude of reservoirs which are adapted to absorb liquid reagents and which have a metering valve at the end/side facing the slots to dose and apply the reagents into the slots; a positioning device to position the reservoirs of the dosing head unit above the respective slots of the slides. | 06-16-2011 |
20110151504 | Methods and Systems for Efficient Automatic Slide Staining in Immunohistochemistry Sample Processing - Automated sample processing systems may include onboard efficient high-speed mixing of at least two components with an automatic vertical force fluidic turbulent component mixer of which a mixed component may be aspirated and high-speed dispensed in a mixing vial. Other aspects may include single sweep applying a multi-treatment cleaning cycle to at least one slide. A multi-treatment cleaning cycle may include a washing treatment and a drying treatment. In yet other aspects the present invention may include an automated recovery sample processing system with the capability of detecting at least one immediate condition of a fortuitously terminated automatic sample processing run and perhaps even an automatic terminated sample processing run reconstruction calculator. | 06-23-2011 |
20110183372 | STIMULATED CELL STANDARDS - Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labeled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labeled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g., carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation. | 07-28-2011 |
20110212486 | Microscope System, Specimen Observation Method, and Computer Program Product - A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye. | 09-01-2011 |
20110250634 | Tissue Container for Molecular and Histology Diagnostics Incorporating a Breakable Membrane - A container for storing a biological sample for molecular diagnostic testing and/or histological testing is provided. The container includes a first chamber for receiving a sample holder therein, a second chamber, and a closure for enclosing the container. A breakable membrane, such as a piercable foil, extends within the container and separates the two chambers. When the breakable membrane is broken, fluid can pass between the first and second chambers. The membrane may be broken through an activator on the closure, such as a depressible member or a rotatable carrier, causing the sample holder to break through the membrane. | 10-13-2011 |
20110287475 | Tissue Fixative Having Increased Viscosity - A gel fixative composition having increased, viscosity which fixes tissues as well as or better than standard liquid fixative solutions. The gel fixative compositions are less prone to leakage and are less likely to splash or spill from containers when they are mishandled. Because of their viscosity, they are less likely to penetrate surfaces and are more easily cleaned up if spilled. A method of making mixing biological fixative by mixing a solution of a biological fixative with a with a high-shear mixer at about 400 to 600 rpm; dispersing uniformly over time about 0.2 to about 2.0 weight percent of thickener while mixing for 2 to 10 minutes; increasing the mixing speed to about 1000 to 1500 rpm; and then, mixing for about 90 to 120 minutes so that a gel fixative composition is formed having a viscosity between about 1,000 cp and 200,000 cp. | 11-24-2011 |
20120009620 | Method for Reducing Entrainment in a Staining Device - During operation of a staining device ( | 01-12-2012 |
20120015399 | FLUORESCENT SOLVATOCHROMIC PIGMENT - The present invention presents a novel fluorescent solvatochromic dye that (1) has an ionic terminal that makes it easier to use in a hydrophilic surface or in polar solvents, (2) can be efficiently excited by commonly used Argon lasers (488 nm), (3) shifts the wavelength of emitted light according to the change of polarity, and (4) can effectively stain living tissues such as cells and the like. | 01-19-2012 |
20120045792 | DRIED BLOOD SPOTTING PAPER DEVICE AND METHOD - A dried blood-spotting device includes a carrier card having a window therethrough along with a punchable glass fiber paper disposed in the window for a uniform absorption of a blood droplet sample into a homogeneous circular sport. The glass fiber paper is configured for enabling improved analysis for small molecules by a liquid chemotography/mass spectroscopy of punched specimens of the sample absorbed glass fiber paper. | 02-23-2012 |
20120122150 | STABILIZATION METHOD FOR BIOLOGICAL SAMPLES BY COMBINATION OF HEATING AND CHEMICAL FIXATION - The present invention provides methods for stabilizing a biological sample for analysis. The invention more particularly provides methods combining heat treatment and chemical fixation of biological samples in order to maintain protein primary structure and post-translational modifications, such as protein phosphorylations. | 05-17-2012 |
20120122151 | CYTOLOGICAL OR HISTOLOGICAL BINDING COMPOSITION AND STAINING METHODS - A histological and cytological fixing composition, includes at least alcohol, ethylene glycol, dimethyl sulfoxide, water, and sodium chloride. Also, process for preparing this fixer, as well as to its use in particular in processes for staining cells or cellular structures are described. | 05-17-2012 |
20120129213 | Multi-Spectral Imaging Including At Least One Common Stain - A method including: providing a sample with M components to be labeled, where M>2; labeling the components with N stains, where N05-24-2012 | |
20120135458 | CLOSED LOOP MONITORING OF AUTOMATED MOLECULAR PATHOLOGY SYSTEM - A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided. | 05-31-2012 |
20120135459 | NOVEL FLUORINATED RHODAMINES AS PHOTOSTABLE FLUORESCENT DYES FOR LABELLING AND IMAGING TECHNIQUES - The present invention relates to novel fluorinated | 05-31-2012 |
20120214194 | PROCESS FOR SYNTHESIZING EPICCONONE ANALOGS - The present invention relates to a process for the total synthesis of epicocconone analogs of formula (I): | 08-23-2012 |
20120219986 | Universal Fixative - This invention provides compositions and methods for fixing biological samples, particularly fecal samples used in the diagnosis of parasitic infection. The fixative composition of the present invention includes a zinc salt, an organic acid and water and permits staining of biological samples without use of toxic compounds, such as formaldehyde and mercury-containing compounds and without the use of additives such as polyvinyl alcohol. The fixative composition and methods arc compatible with many diagnostic assays, including trichrome stains, iron hematoxlin, ELISA, fluorescent assays, and lateral flow assays. | 08-30-2012 |
20120219987 | DEVICE FOR ELECTROPORATION AND LYSIS - There is provided a membrane disruption device including a sample container and an electrode assembly. The sample container includes a sample-containing space defined by a containing surface and configured for containing a cellular-material comprising fluid, wherein the sample-containing space includes at least one membrane disruption space. The electrode assembly including a first electrode portion spaced apart from a second electrode portion, for generating an electric field within the membrane disruption space and effecting membrane disruption of a cell of a cellular material-comprising fluid disposed within the membrane disruption space. | 08-30-2012 |
20120264165 | METHOD OF DETECTING TUMOR CELLS BY FLUORESCENCE SIGNALS - The invention relates to a method of detecting dividing cells, or cells having the potential of dividing, in a cytological specimen stained using a Papanicolaou staining process, by the detection of a fluorescence signal coming from the membranes of these cells. | 10-18-2012 |
20120270262 | METHODS FOR MOBILE ZINC MEASUREMENT - This invention relates to a method for using a zinc sensor compound to detect a disease associated with the disruption of zinc homeostasis, such as prostate cancer. The zinc sensor compound comprises an optical reporter having two or more recognition units where each of the recognition units is capable of associating with at least one zinc ion. | 10-25-2012 |
20120322099 | FIXATIVE AND STAINING SOLUTIONS - The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. | 12-20-2012 |
20120329088 | PROCESSING SYSTEM FOR PROCESSING SPECIMENS USING ACOUSTIC ENERGY - A method for fixing a biological sample includes delivering energy through a biological sample that has been removed from a subject, while fixing the biological sample. A change in speed of the energy traveling through the biological sample is evaluated to monitor the progress of the fixation. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A computing device can evaluate the speed of the energy based on signals from the receiver. | 12-27-2012 |
20130023008 | HISTOLOGICAL METHOD - The invention relates to the technical field of the histological preparation of biological tissue comprising a method and means for preparing transparent biological specimens for examination under a light microscope. | 01-24-2013 |
20130029375 | BIOLOGICAL FIXATIVE AND METHOD OF USING THE BIOLOGICAL FIXATIVE - A composition that includes an aldehyde, alcohol, and a ketone, the volumetric ratio of the alcohol to the ketone in the composition ranging from as low as about 0.8:1 to as high as about 4.5:1 and the volumetric ratio of the alcohol to the aldehyde in the composition ranging from as low as about 41.5:1 to as high as about 450:1. | 01-31-2013 |
20130045503 | CLEARING REAGENT FOR BIOLOGICAL MATERIAL, AND USE THEREOF - A clearing reagent according to this invention for making a biological material transparent is a solution containing, as an active component, at least one compound selected from the group consisting of urea and urea derivatives, in order to provide a novel clearing reagent for making a biological material transparent. | 02-21-2013 |
20130059330 | FIXING SOLUTION FOR BIOLOGICAL CELLS - This fixing solution is intended to preserve in vitro a cytological sample, comprising nucleated cells and erythrocytes and comprises alcohol for fixing the cells. It comprises physiological saline in order to avoid osmotic shocks at the wall of the cells, formalin and polyethylene glycol for retaining the size and the integrity of the nucleated cells and erythrocytes, which are fixed in said solution. | 03-07-2013 |
20130102027 | METHOD FOR DETECTING CANCER CELLS USING VERTICALLY CARBON NANOTUBES - The various embodiments herein provide a method for detecting the cancerous cells using the carbon nanotubes. The method comprises preparing a solution of the tissue cells. The prepared solution of the tissue cells is poured on a fabricated substrate to carry out an entrapment of the tissue cells on the substrate. The substrate is dried after the entrapment in an air ambient and observed under a scanning electron microscope. The cancer cell is detected based on the biomechanical properties such as softness, deformability and an elasticity of the cancer cells. The cancer cell is detected based on the deflection of the substrate due to the entrapment of the cancer cells. | 04-25-2013 |
20130115652 | TISSUE ARRAY PRODUCTION METHOD - A tissue array production method which enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. | 05-09-2013 |
20130137136 | METHOD AND SYSTEM FOR AUTOMATED DEPARAFFINIZATION AND NON-IMMUNOHISTOCHEMICAL SPECIAL STAINING OF TISSUE SAMPLES - Disclosed are methods and systems for automated deparaffinization and histochemical staining of tissue samples. Samples are automatically deparaffinized and stained without the use of harsh chemicals and without the use of extreme temperatures. | 05-30-2013 |
20130189729 | FIXATIVE AND STAINING SOLUTIONS - The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. | 07-25-2013 |
20130196370 | FIXATIVE AND STAINING SOLUTIONS - The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. | 08-01-2013 |
20130203109 | Methods and Compositions for Hematoxylin and Eosin Staining - The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over—such as carried-over into hematoxylin—will not significantly influence the pH of the next staining reagent. | 08-08-2013 |
20130210070 | MICROFLUIDIC DEVICE AND METHOD FOR CONTROLLING INTERACTION BETWEEN LIQUIDS - A microfiuidic device comprises a valve having electrically controllable wetting properties. The valve comprises a valve surface arranged in a closed valve space defined by at least the valve surface, a first liquid opening for leading a first liquid to the valve and a second liquid opening for leading a second liquid to the valve. The valve surface, in a first state, is sufficiently hydrophobic to prevent contact between the first liquid and the second liquid. The valve surface, in a second state, is sufficiently hydrophilic to allow contact between the first liquid and the second liquid. A ventilation outlet is provided for allowing gas to escape from the valve space. | 08-15-2013 |
20130217065 | METHOD IN THE PREPARATION OF SAMPLES FOR MICROSCOPIC EXAMINATION, AND APPARATUS FOR CHECKING THE COVERSLIPPING QUALITY OF SAMPLES - The invention relates to a method in the preparation of samples for microscopic examination onto which a coverslip is applied. The method is notable for the fact that the coverslipping quality is checked automatically and at least partly optically. The invention further relates to an apparatus for carrying out the method, and to an apparatus for checking the coverslipping quality of samples onto which a coverslip is applied. | 08-22-2013 |
20130252278 | PROCESS OF APPLICATION PROCEDURES THROUGH CELL BIOLOGY FOR ANIMAL USE - Process of application procedures through cell biology for animal use, the invention is applied to the field of cell biology, specifically the isolation, in vitro culture, cryopreservation, cell differentiation and application of mesenchymal stem cells derived from adipose tissue of animals for autologous cell therapy purposes. Strategically seeks to establish, from the adipose tissue collection, all events resulting in schematic form, in order to obtain a homogeneous extract of mesenchymal stem cells. In this invention, it is proposed a dynamic which allows the use of mesenchymal stem cells in veterinary cell therapy found in centers distant from the cell culture laboratory, since the transportation time of tissue and mesenchymal stem cells does not exceeds 78 hours after the their processing. | 09-26-2013 |
20130252279 | FIXATIVE AND STAINING SOLUTIONS - The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. | 09-26-2013 |
20130260418 | CELL-BASED ANTIOXIDANT PROTECTION ASSAY - Methods are provided herein for determining antioxidant activity of a test sample in intact cells. The method includes determining the antioxidant capacity of a test sample in intact red blood cells, wherein the test sample is added to intact red blood cells and oxidative damage is measured by alteration of fluorescence intensity of an oxidation-sensitive fluorescent indicator dye. | 10-03-2013 |
20130302851 | SYSTEM AND APPARATUS FOR ANALYSIS OF A GUAYULE PLANT IN SITU - A system and method for in-field near infrared spectroscopy (NIRS) analysis of rubber and resin concentrations a guayule plant is provided. The system includes a wagon or other vehicle with the NIRS device mounted on the wagon. A computer or processor electrically coupled to the NIRS device is also housed within an area or extension of the wagon. During measurement of a guayule plant in the field, a guayule plant covering is placed over the guayule plant and a light shield coupled to the NIRS device is inserted into an opening on the guayule plant covering. The NIRS device is configured to perform a reading of the guayule plant within the plant covering and communicate results of the reading to the computer. A calibration equation is then preferably applied to the guayule plant readings to produce the rubber and resin concentrations of the guayule plant. | 11-14-2013 |
20130309715 | PENTAMETHINE CYANINE FLUORESCENT DYES, PREPARATION METHODS AND USES THEREOF - A pentamethine cyanine fluorescent dye having the general formula I, a synthetic method and a use thereof are disclosed. In the general formula, X is CHO or CHCR | 11-21-2013 |
20130337502 | ANALYSIS OF MICROBES FROM MICROCOLONIES BY MALDI MASS SPECTROMETRY - The invention relates to the cell disruption of microbes and the preparation of the microbe proteins for mass spectrometric analysis. The cells of microbes from microcolonies are disrupted by physical or chemical means directly on the nutrient medium. The released proteins are then transferred to sample supports by direct contact with their contact surfaces; electrophoresis can be used for assistance. Once the the proteins are firmly adsorbed on the contact surfaces, they can be washed with water in order to remove substances which interfere with the ionization process. For analysis by matrix-assisted laser desorption (MALDI), the proteins are prepared on the contact surfaces of the sample supports with matrix substances to form MALDI samples; the sample supports are then introduced into a MALDI mass spectrometer for the acquisition of the mass spectra. The microbes are identified by similarity comparisons between the mass spectra of the microbe proteins and similarly obtained reference spectra. | 12-19-2013 |
20140004561 | AUTOMATED SYSTEMS AND METHODS FOR PREPARING BIOLOGICAL SPECIMENS FOR EXAMINATION | 01-02-2014 |
20140004562 | REVERSIBLY WATER-SOLUBLE NANOCRYSTALS | 01-02-2014 |
20140038232 | Enhanced Scheduling Sample Processing System and Methods of Biological Slide Processing - A sample processing system | 02-06-2014 |
20140051118 | Two Phase Immiscible System for the Pretreatment of Embedded Biological Samples - The present application provides a two phase immiscible system for the pretreatment of embedded biological samples comprising placing at least one support having an embedded biological sample on its surface into a pretreatment container, adding to the pretreatment container at least one reagent forming a layer, adding a carrier composition to the pretreatment container, such that reagent forming layer is formed on the top of the carrier composition, and in an amount such that the at least one reagent forming layer contacts at least a portion of the embedded biological sample. Pretreatment of the embedded biological samples can include removal of embedding medium from embedded biological, target retrieval and enzyme blocking samples before staining histochemical analysis or other processes. The system also includes an apparatus and processes of automation of the pretreatment methods. | 02-20-2014 |
20140073003 | METHOD FOR STAINING A HISTOLOGICAL SAMPLE, AND AUTOMATED STAINER - The invention relates inter alia to an automated stainer for staining, in particular for hemalum-eosin (HE) staining, of a histological sample, the automated stainer exposing the sample to the action of at least one stain using at least one staining parameter. The automated stainer is notable for the fact that the automated stainer comprises an input means with which a staining parameter is definable, in particular is inputtable or is selectable from a plurality of possible staining parameters; and that a control apparatus is present which, upon application of the defined staining parameter, ascertains the prospectively expectable outcome of an action and displays it to the user with a display apparatus, before the automated stainer actually stains the sample. | 03-13-2014 |
20140087419 | METHOD FOR MAKING BIOLOGICAL MATERIAL TRANSPARENT AND USE THEREOF - A method for making a biological material transparent according to the present invention includes: a first permeation step of causing a first solution to permeate into a biological material, the first solution containing at least one compound selected from the group consisting of urea and urea derivatives at a predetermined concentration; and then a second permeation step of causing a second solution to permeate into the biological material, the second solution containing at least one compound selected from the group consisting of urea and urea derivatives at a concentration higher than the concentration of the at least one compound contained in the first solution. | 03-27-2014 |
20140147883 | FILTER SUPPORT WITH A PHASE-CHANGING MEDIUM - The present invention relates to a method for processing isolated cells, in particular rare cells such as circulating tumor cells, to render them suitable for optical imaging, comprising the steps of (a) depositing filter material comprising captured cells on a phase-change medium; (b) melting said phase-change medium until the medium is spread out below said filter material; and (c) lowering the temperature of said phase-change medium until the medium solidifies, resulting in an optically flat filter comprising said cells at a fixed position. The method may additionally comprise an initial step of capturing a cell on a filter material. The phase-change medium, preferably paraffin wax, may comprise a porous, or mesh-like structure allowing the passage of a fluid through the medium. The phase-change medium may further be mounted on a carrier such as a glass carrier or a polymer material carrier. The present invention further relates to processed filter material comprising isolated cells suitable for optical imaging, which is obtainable by the method; the use of such filter material for diagnostic, histological, microbiological, biochemical, oncologic, or hematologic analysis, as well as a device for isolating cells or processing cells to render them suitable for optical imaging. | 05-29-2014 |
20140154736 | METHODS FOR SAMPLE STORAGE AND DEVICE THEREOF - A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C. | 06-05-2014 |
20140162311 | CYTOBLOCK PREPARATION SYSTEM AND METHODS OF USE - An apparatus and method that may be used for collecting target cells or tissue and preparing a cell block are disclosed. | 06-12-2014 |
20140178927 | CLARIFYING REAGENT FOR BIOLOGICAL MATERIALS AND USE THEREOF - A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %. | 06-26-2014 |
20140212918 | IN VITRO MODEL OF MACROSTEATOTIC (FATTY) LIVER - The present invention relates to a system and methods for identifying a compound for de-fatting and functional recovery of macrosteatotic hepatocytes. | 07-31-2014 |
20140248658 | CYTOPLASMIC STAIN COMPOSITION - A composition of two cytoplasm stains for use in differentiation of cell nuclei from cell cytoplasmic components and differentiation of specific cytoplasm components by tinctorial contrast. Specifically, the invention relates to two stain compositions utilized as a counter stain in the hematoxylin and eosin procedure for use in histological and cytological microscopic evaluation of tissue and cells. In one embodiment, the composition includes eosin-Y as the sole dye in the composition, and a propylene glycol solvent and an organic buffer. In another embodiment, the composition includes a mixture of eosin-Y and phloxine-B as the sole dye, also in a propylene glycol solvent and an organic buffer. | 09-04-2014 |
20140273078 | APPARATUS FOR WORKING ON HISTOLOGICAL SAMPLES - The invention relates to an apparatus for treatment of histological samples. The apparatus includes at least a working station, in which the sample gets into contact with a liquid reagent. Further, the apparatus includes a purification device, which blows off at least a part of the reagent adhering to the sample and/or the sample holding arrangement by means of a gas flow, or which sucks off at least a part of the reagent adhering to the sample and/or a sample holding arrangement. | 09-18-2014 |
20140273079 | BIOLOGICAL SPECIMEN HANDLING APPARATUS AND METHOD - In accordance with one aspect of the invention, a mold is provided for receiving a tissue specimen and molten embedding material that improves the ease of withdrawing the embedded tissue specimen. The mold has a barrier coating intimately bonded to one or more surfaces of the mold that repels the attraction of the embedding material to the one or more mold surfaces and produces a positive meniscus of the embedding material in the mold. The barrier coating permits the mold to be re-used without needing to manually re-apply a release agent to the mold before using the mold. Further, the barrier coating permits the use of more aggressive draft angles than conventional molds by reducing the frictional engagement between the embedding material and the mold cavity surfaces. | 09-18-2014 |
20140273080 | METHOD FOR METABOLOMIC SAMPLE PREPARATION BASED ON IONIC LIQUID DISPERSIVE LIQUID-LIQUID MICROEXTRACTION - Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided. | 09-18-2014 |
20140273081 | METHOD AND COMPOSITION FOR STAINING AND SAMPLE PROCESSING - The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde. | 09-18-2014 |
20140273082 | METHOD AND COMPOSITION FOR STAINING AND PROCESSING A URINE SAMPLE - The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300. | 09-18-2014 |
20140349336 | SAMPLE VIAL FOR DIGITAL HOLOGRAPHIC ANALYSIS OF A LIQUID CELL SAMPLE - The current invention concerns a sample vial for receiving a liquid cell sample, to be used in conjunction with a digital holographic microscope (DHM), said sample vial comprises at least two compartments in fluid connection with one another, said compartments comprising at least one pair of screening surfaces, said screening surfaces are essentially flat; and characterized in that the distance between the pair of screening surfaces of the second compartment is smaller than the distance between the pair of screening surfaces of the first compartment. In a second and third aspect, the current invention pertains to a method and system for analyzing a liquid cell sample by DHM, employing the sample vial of the current invention. | 11-27-2014 |
20140349337 | RAMAN SPECTROSCOPY FOR DETECTION OF GLYCATED ANALYTES - The present invention relates to the optical measurement of blood analytes, such as glycated hemoglobin (HbA1c) and serum albumin as a functional metric of mean blood glucose in the diagnosis of diabetic patients. Non-enhanced Raman spectroscopy is employed as the analytical method for quantitative detection of blood analytes. Using processing techniques, non-enzymatic glycosylation (glycation) of the analytes results in measurable and highly reproducible changes in the acquired spectral data, which enable the accurate measurements and classification of glycated and unglycated analytes. | 11-27-2014 |
20140363843 | SYSTEMS AND METHODS FOR ACOUSTICALLY PROCESSING TISSUE SAMPLES - Systems and methods for processing tissue samples using acoustic energy. The tissue sample may be collected and placed on a substrate (e.g., microscope slide), or other sample holder. A transfer substrate may be positioned on the other side of the sample opposite the substrate. A microscope incorporated with the acoustic treatment system may be used to view, identify and select a portion of the sample to be transferred from the initial substrate to the transfer substrate. The selected portion of the sample is exposed to focused acoustic energy while disposed between the two substrates. The focused acoustic energy has characteristics (e.g., high frequency, small focal zone) that may be effective to transfer the selected portion of the sample from the initial substrate to the transfer substrate. Such transfer may occur with little to no damage to the sample, for example, at low temperature isothermal conditions and/or little to no cavitation of or around the sample. | 12-11-2014 |
20150037836 | TEMPERATURE CONTROLLED DISSECTION AND OBSERVATION STAGE - A temperature controlled tissue dissection and observation stage comprises a central working area and a first temperature controlled station positioned within the central working area. The first temperature controlled station comprises a first temperature controlling element configured to adjust the temperature of the first temperature controlled station. The stage further comprises a user input device in communication with the first temperature controlling element. In one embodiment, the stage comprises four temperature controlled stations. Three of these stations are designed to house petri dishes filled with solutions required for tissue restoration and reconstruction surgeries, and the fourth station is configured to function as a flat cutting surface for the user. In one embodiment, thermostatic control over the system is achieved through the integration of thermoelectric coolers, a microcontroller, and feedback temperature sensors. | 02-05-2015 |
20150087018 | CASSETTE - A histology processing cassette comprising a box comprising a compartment for holding a biological tissue sample, the box having a bottom face comprising at least in part a sample support surface and being transmissible to radiation, an open top face, and two side walls, a back wall and a front wall, the box having dimensions greater than a standard size histology processing cassette and comprising a recess in the front wall adapted to receive a standard size cassette which has a front wall comprising a unique identifier for the biological tissue sample such that upon insertion of the standard cassette in the recess the unique identifier on the front wall of the standard cassette is readable. | 03-26-2015 |
20150104826 | DEVICES AND CARTRIDGES FOR EXTRACTING BIO-SAMPLE REGIONS AND MOLECULES OF INTEREST - Methods, devices, and systems for integrating extraction and purification of bio-sample regions and materials with patient analysis, diagnosis, follow up, and treatment. The invention provides a means to insert disclosed substrates, cartridges, and cartridge-processing instrument or instruments into a standard clinic or pathology laboratory workflow. Specifically, we disclose methods, devices, and systems for inserting standard pathology slides into disclosed cartridges and cartridge-processing instruments, either manually, semi-automatically, automatically, or by robotic means. | 04-16-2015 |
20150132797 | COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING - Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups. | 05-14-2015 |
20150132798 | INTEGRATED SEQUENTIAL SAMPLE PREPARATION SYSTEM - The invention provides an integrated sequential sample preparation system using a sequential centrifuge for preparing samples for analysis. Methods of more efficiently preparing discrete samples sequentially for subsequent analysis are also provided. The apparatus and methods for sequentially preparing discrete samples provide improved operating efficiencies over conventional preparation processes that use batch centrifugation systems. Such advantages include reducing dwell time, increasing system throughput, reducing sample preparation system footprint, and improving precision of the analytical process. The integrated sequential preparation system with the integrated sequential centrifuge further provides the capability of handling critical or STAT samples without compromising the operating efficiencies achieved by preparing discrete samples in a sequential manner. | 05-14-2015 |
20150140601 | FIXING TISSUE SAMPLES USING NITROGEN-CONTAINING COMPOUNDS THAT RELEASE ALDEHYDES | 05-21-2015 |
20150147778 | CRYO-PREPARATION SYSTEMS AND METHODS FOR NEAR-INSTANTANEOUS VITRIFICATION OF BIOLOGICAL SAMPLES - A sample vitrification system includes a capsule structure configured for carrying a biological sample within a compartment while the sample is subjected to ultra-rapid freezing by way of a cryogenic coolant jet, and while the sample is exposed to pulsed microwave radiation in a manner that disrupts water molecule pentamer formation and which disrupts initial ice crystal nucleation events within the compartment within tens of microseconds to provide a vitrification depth within the compartment of tens of microns or more. The sample can reside between a very high thermal conductivity substrate and a cover. The cryogenic coolant jet is applied to the substrate from beneath the sample. The cover can carry a set of microwave excitation elements configured for providing microwave radiation to internal portions of the compartment. Portions of the capsule structure's interior can be imaged during microwave assisted jet freezing, such as by way of an optical microscope. | 05-28-2015 |
20150147779 | Clearing Agent and Mounting Medium for Microscopy - A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and/or a stain. The solution can also comprise derivatives and/or analogs of 2,2,2-trichloroethanol and/or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting. The solution can be used with specimens or tissues/cells/parts originating from animals, poultry, livestock, humans, higher plants, yeasts, molds, microorganisms, insects, mites, or reptiles. | 05-28-2015 |
20150292990 | Accelerated Wright-Giemsa and May-Grunwald Staining Methods - The present disclosure provides methods for carrying out Romanowsky-type stains, specifically Wright-Giemsa and May-Grünwald stains, quickly and efficiently. The methods greatly reduce the overall amount of time required to complete a Wright-Giemsa stain or a May-Grünwald stain of sufficient quality on a biological sample. The subject methods can be applied to both manual and automated staining procedures. | 10-15-2015 |
20150300930 | Tissue Sample Preparation, and MALDI MS Imaging Thereof - Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis. | 10-22-2015 |
20150343445 | METHOD OF PRODUCING A REAGENT ON-BOARD AN INSTRUMENT - The present invention relates broadly to production of a reagent on-board an instrument. The instrument is provided with one or more mixing wells | 12-03-2015 |
20160003718 | AID FOR FILLING LIQUID, AND METHOD FOR FILLING LIQUID - A liquid filling aid that is placed on a plate-shaped member and defines a reaction chamber to be filled with a liquid, the aid includes a main body, a storage section that is formed in the main body and stores the liquid, a reaction section that is a recess formed at a bottom of the main body, a communication aperture for fluid communication between the storage section and the reaction section, and an air vent for communication between the reaction section and outside air, wherein the reaction section and an upper surface of the plate-shaped member define the reaction chamber and a liquid filling method including a step of placing the liquid filling aid on the plate-shaped member, and a step of discharging the liquid in an amount equal to or larger than the volume of the reaction chamber for supply to the storage section. | 01-07-2016 |
20160011175 | Methods for Identifying Stem Cells by Detecting Fluorescence of Cells and Syncytia | 01-14-2016 |
20160018300 | STAINING AGENT FOR STAINING TISSUE, PRODUCTION METHOD FOR STAINING AGENT FOR STAINING TISSUE AND TISSUE STAINING KIT INCLUDING STAINING AGENT FOR STAINING TISSUE - An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less. | 01-21-2016 |
20160018301 | Automated Staining and Decolorization of Biological Material - An improved method and apparatus for staining samples of biological material for accurate analysis of the sample. Biological material is applied to a substrate, such as a microscope slide. The biological specimen is then stained with a selected staining composition, which may be gentian violet for a Gram's Stain analysis. The stained biological material is at least partially decolorized and the level of decolorization is analyzed optically. If necessary, the decolorizing step and the optical analysis steps are repeated until a selected level of decolorization is obtained. | 01-21-2016 |
20160033373 | BIOLOGICAL SAMPLE COLLECTION AND PRESERVATION - An embodiment of the claimed invention is directed to a method that greatly streamlines and reduces costs for tissue preparation, preservation, long-term storage and sample retrieval for molecular analysis using a method based on dried blood spot (DBS) technology. In this method, a small needle punch sample of freshly excised tissue will be homogenized in stabilizing reagent and inserted into a device containing absorbent material and drying agent. This device is suitable for long-term sample storage at ambient temperature and allows for easy removal of sections for biomarker analysis. | 02-04-2016 |
20160069809 | Cervical Sample Preparation For Reduced Variability In Raman Spectroscopy - A method of performing a spectroscopy process on a fixed biological sample is described. The method comprises the steps of: (i) treating the fixed biological material to cause oxidation of haemoglobin present in the fixed biological material, and (ii) performing spectroscopy on the treated fixed biological sample. The method is particularly suitable for cervical sample preparation for reduced variability in Raman spectroscopy. | 03-10-2016 |
20160109337 | METHOD AND SYSTEM FOR INDICATING TIME ON A REAGENT CONTAINER - An apparatus, system and method for monitoring fixation of a biological sample within a container. The apparatus system or method including a container having a fixation medium for fixation of a biological sample placed within the container and a label attached to the container, the label having a time indicator operable to automatically indicate a processing time of a biological sample placed within the fixation medium. | 04-21-2016 |
20160139010 | System and Method for Processing Biological Specimens - A system for processing biological specimens mounted on microscope slides by adding and removing processing fluids from microscope slides by means of capillary action using a slide holder capable of holding multiple microscope slides, and a spacer positioned in between two slides of a slide pair to create a capillary gap. A capillary gap adjuster can be used to pinch and release one end of the slide pair to create a pulsatile action to mix the reagent within the capillary gap. The system may further include a reagent holder, an absorbent pad, a series of reagent baths, and an incubator for holding one or more slide holders. | 05-19-2016 |
20160157837 | Container and Method for Collecting, Transporting and Storing Biological Tissue Samples - The invention refers to a container ( | 06-09-2016 |
20160169776 | METHOD FOR RENDERING BIOLOGICAL MATERIAL TRANSPARENT AND PROCESSING KIT FOR RENDERING BIOLOGICAL MATERIAL TRANSPARENT | 06-16-2016 |
20160377514 | Clearing Agent and Mounting Medium for Microscopy - A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and/or a stain. The solution can also comprise derivatives and/or analogs of 2,2,2-trichloroethanol and/or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting. The solution can be used with specimens or tissues/cells/parts originating from animals, poultry, livestock, humans, higher plants, yeasts, molds, microorganisms, insects, mites, or reptiles. | 12-29-2016 |
20160377515 | METHOD FOR STAINING A HISTOLOGICAL SAMPLE, AND AUTOMATED STAINER - The invention relates inter alia to an automated stainer for staining, in particular for hemalum-eosin (HE) staining, of a histological sample, the automated stainer exposing the sample to the action of at least one stain using at least one staining parameter. The automated stainer is notable for the fact that the automated stainer comprises an input means with which a staining parameter is definable, in particular is inputtable or is selectable from a plurality of possible staining parameters; and that a control apparatus is present which, upon application of the defined staining parameter, ascertains the prospectively expectable outcome of an action and displays it to the user with a display apparatus, before the automated stainer actually stains the sample. | 12-29-2016 |
20190145868 | METHODS FOR REVERSIBLE AND TUNABLE TISSUE MAGNIFICATION | 05-16-2019 |
20220136937 | SILVER NANOPARTICLE SURFACE ENABLED SELF-ASSEMBLY OF ORGANIC DYE MOLECULES - Fluorescence titration of methylene blue, rhodamine B and rhodamine 6G (R6G) by silver nanoparticle (AgNP) all resulted in an initial steep quenching curve followed with a sharp turn and a much flatter quenching curve. At the turn, there are about 200,000 dye molecules per a single AgNP, signifying self-assembly of approximately 36 layers of dye molecules on the surface of the AgNP to form a micelle-like structure. These fluorescence-quenching curves fit to a mathematical model with an exponential term due to molecular self-assembly on a AgNP surface, or “self-assembly shielding effect”, and a Stern-Volmer term (nanoparticle surface enhanced quenching). Such a “super-quenching” by AgNP can only be attributed to “pre-concentration” of the dye molecules on the nanoparticle surface that yields the formation of micelle-like self-assembly, resulting in great fluorescence quenching. Overall, the fluorescence quenching titration reveals three different types of interactions of dye molecules on AgNP surface: 1) self-assembly (methylene blue, rhodamine B and R6G), 2) absorption/tight interaction (tryptamine and fluorescein), and 3) loose interaction (eosin Y). We attribute the formation of micelle-like self-assembly of these three dye molecules on AgNP to their positive charge, possession of nitrogen atoms, and with relatively large and flat aromatic moieties. | 05-05-2022 |