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Determining presence or kind of micro-organism; use of selective media

Subclass of:

435 - Chemistry: molecular biology and microbiology

435004000 - MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP

435029000 - Involving viable micro-organism

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435039000 Quantitative determination 121
435036000 Streptococcus; staphylococcus 14
435038000 Enterobacteria 4
20100120086MEDIUM FOR DETECTING AND DIFFERENTIATING VANCOMYCIN-RESISTANT ENTEROCOCCI - A medium for the growth of vancomycin-resistant 05-13-2010
20090280524SOLID CULTURE MEDIUM FOR THE DETECTION AND/OR THE SPECIES DISCRIMINATION OF GLYCOPEPTIDE-RESISTANT ENTEROCOCCI - The present invention relates to a solid culture medium for the detection and/or discrimination, among glycopeptide-resistant enterococci, of the groups of species 11-12-2009
20100255530BACTERIA DETECTION AND/OR IDENTIFICATION MEDIUM - The invention relates to a method for detecting and/or identifying 10-07-2010
20120301917HYDROPHILIC IR TRANSPARENT MEMBRANE, SPECTROSCOPIC SAMPLE HOLDER COMPRISING SAME AND METHOD OF USING SAME - The present invention features hydrophilic IR-transparent porous membranes, particularly hydrophilic IR-transparent porous polyethylene membranes and methods of preparing the hydrophilic membranes by treatment of hydrophobic IR-transparent porous membranes with plasma. The present invention further features spectroscopic sample holders which incorporate the hydrophilic IR-transparent porous membranes and methods of identifying bacteria and other microorganisms in samples by infrared spectroscopy.11-29-2012
435035000 Using radioactive material 2
20100003715Biological agent detection and identification system - A sample under test containing non-live and/or live particulates is subject to optical excitation on a single particle-by-particle basis or as a small group of particulates sufficient to induce a subsequent fluorescence emission that is observed for a selected period of time by a sensor, typically a photomultiplier tube. The output of the sensor is representative of the intensity or amplitude of the fluorescence emission while the decrease in that intensity or amplitude with time is representative of the decay rate of the fluorescence emission. Those particulates exhibiting a decay rate “faster” than a threshold decay rate, which is determined empirically for the class of biological agents of interest, are identified as living while those particulates exhibiting decay rate “slower” than a threshold decay rate, which is also determined empirically for the class of biological agents of interest, are identified as a non-live interferant.01-07-2010
20130130309Radiopharmaceutical Production System and Quality Control System Utilizing High Performance Liquid Chromatography - HPLC-based quality control systems to perform quality control testing on a radiopharmaceutical solution shortly after synthesis. An HPLC-based quality control system makes efficient use of sample volume and is compatible with a variety of radioisotopes and radiopharmaceutical compounds. In several embodiments, the automated nature of an HPLC-based quality control system allows for quality control tests to be conducted quickly and with minimal impact on user workflow. When used as part of an integrated PET biomarker radiopharmaceutical production system, the present general inventive concept permits a manufacturer to produce product and conduct quality control tests with lower per dose costs.05-23-2013
Entries
DocumentTitleDate
20130045502Method for Detection or Identification of Bacteria or Bacterial Spores - A method and apparatus for detecting bacteria and bacterial spores are disclosed in which a sample is assessed for the presence of bacteria and/or bacterial spores by reference to horizontally polarized fluorescent light.02-21-2013
20130045501Detection Device for Detecting Analytes in Liquid Specimen - A detection device for detecting analytes in liquid specimen is provided. The detection device comprises: a specimen chamber for collecting or storing a liquid specimen; a detecting chamber for containing a detecting element; and a through hole for transferring the liquid specimen between the specimen chamber and the detecting chamber. The through hole can be opened or self-sealed. The sealing or opening of the through hole controls whether or not the liquid specimen in the specimen chamber enters the detecting chamber via the through hole. Furthermore, a detection method is provided.02-21-2013
20110207167METHOD OF ISOLATING A CELL FROM URINE - A method of isolating a cell from a urine sample includes dialyzing the urine sample with a dialysate. The dialysate is separated from the urine sample by a dialysis membrane. The dialysis membrane excludes the passage of the cell through the dialysis membrane.08-25-2011
20090239256Detection of Micro-Organisms - The present invention relates to a method of detecting the presence of a viable micro-organism of interest in a sample containing a contaminating micro-organism.09-24-2009
20080293095Polydiacetylene-Containing Solid Colorimetric and/or Fluorescent Detector, Method for Its Preparation and Uses Thereof - A colorimetric and/or fluorescent detector, which comprises a film of polydiacetylenes and lipids deposited on a substrate, wherein said film is coated with one or more layers, wherein at least one of said layers is capable of supporting the growth of microorganisms. Also provided are processes for preparing the novel colorimetric and/or fluorescent detector and uses thereof in the detection of bacteria.11-27-2008
20080261264Culture Medium for Detecting of Clostridium Perfringens - The invention relates to a culture medium for the detection of 10-23-2008
20080268493Identification Method Based On Surface-Enhanced Raman Scattering - This invention is related to a reproducible identification method based on surface-enhanced Raman scattering (SERS), in which bacterial samples are used in identification of bacteria by mixing with the concentrated silver and gold nanoparticles.10-30-2008
20120183992Reagents and Methods for Classifying Leukocytes - A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.07-19-2012
20090191585CELL IMAGE PROCESSING APPARATUS, CELL IMAGE DISPLAYING SYSTEM AND CELL IMAGE DISPLAYING METHOD - A cell image processing apparatus includes a large area image obtainer for obtaining a large area image which is obtained by imaging an area including a white blood cell and a red blood cell, a processing part for obtaining a portion image including the white blood cell from the large area image, and a memory for storing the portion image as an image for monitoring white blood cell and storing the large area image as an image for monitoring red blood cell.07-30-2009
20100120084REACTION MEDIUM FOR IDENTIFYING/DETECTING MICROORGANISMS - The present invention relates to a reaction medium for identifying/detecting microorganisms, that comprises at least one active molecule encapsulated in a cyclodextrin.05-13-2010
20130078668METHOD AND APPARATUS FOR DETECTING THE PRESENCE OF INTRAERYTHROCYTIC PARASITES - An apparatus and method for determining the presence of an intraerythrocytic organism within a sample of liquid whole blood is provided. The method includes the steps of a) creating an image of the sample quiescently residing within a sample chamber; b) evaluating the image for the presence of at least one red blood cell within the image containing at least one first region of decreased hemoglobin content and/or hemoglobin concentration within the red blood cell, which red blood cell has an otherwise homogenous distribution of hemoglobin; and c) determining the presence of an intraerythrocytic organism within the sample using the evaluation of the image.03-28-2013
20130078667METHODS FOR DETECTING AND COLLECTING CIRCULATING TUMOR CELLS - The present invention is directed towards methods of expanding circulating tumor cell populations and methods of detecting circulating tumor cells in a sample.03-28-2013
20100075368Media for recovery of microorganism in the presence of antibiotics - The present invention provides a media and a method of using such as media for the bioburden or sterility testing of antibiotics or environmental testing of antibiotic manufacturing areas. The medium contains one or more divalent or trivalent cation constituents, preferably of magnesium, calcium, aluminum and iron that allow for microorganism growth even in the presence of residual antibiotic.03-25-2010
20120244570METHODS FOR DETERMINING MICROBIAL CONTAMINATION OF ALLOGRAFT PRODUCTS - A method for determining microbial contamination of allograft products comprises providing an extraction vessel having an outer container and a removable inner basket, place the allograft product into the inner basket, providing an abstraction fluid in the outer container of the extraction vessel, positioning the inner basket in the outer container, so that the allograft product and the inner basket is submerged in the extraction fluid and agitating the extraction fluid for a predetermined amount of time and analyzing the extraction fluid from microbial contamination.09-27-2012
20120244569QUANTUM DOT CARRIER PEPTIDE CONJUGATES SUITABLE FOR IMAGING AND DELIVERY APPLICATIONS IN PLANTS - Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.09-27-2012
20130078669Method And Devices For The Cross-Referencing Of Identification Of Tissue Slice Supports For Microtomised Analytical Samples - The invention relates to a method and device for the cross-referencing of identification (03-28-2013
20090317859CALORIMETRIC ASSESSMENT OF MICROORGANISMS AND USE THEREOF - Methods, integrated kits and systems for the rapid detection, identification and/or quantification of microorganisms in samples such as blood samples via calorimetry are disclosed.12-24-2009
20130084598Method for Detecting Clusters of Biological Particles - The invention relates to a method for detecting clusters of biological particles (04-04-2013
20130084597COMPRESSED GAS MICROBIAL TEST UNIT - Provided is a microbial test unit for detecting microbes in a compressed gas. The microbial test unit includes a decompression region and a sampling region in fluid communication with the decompression region and separated from the decompression region by a plate. The decompression region and the sampling region define a flow path. An inlet communicates with the decompression region such that compressed gas entering the decompressing region is incident on the plate for effectuating decompression of the gas prior to the gas passing into the sampling region. The microbial test unit may be used in conjunction with a growth medium substrate to form a microbial test system. The microbial test unit provides the ability to test compressed gas directly input to the unit due to positive pressure from the compressed gas pushing the microbes onto the microbial growth substrate.04-04-2013
20130040337BLOOD ANALYSIS APPARATUS AND BLOOD ANALYSIS METHOD - As a result of acquiring visible absorption spectra of red blood cells and analyses of the spectra, inventors of the present disclosure have discovered the fact that echinocytes show a characteristic spectrum pattern having an absorption peak in a wavelength region of 450 to 490 nm between Soret and Q bands. Then, the present disclosure provides a blood analysis apparatus having an analysis section for detecting the absorption peak of a visible absorption spectrum acquired for blood. In accordance with this blood analysis apparatus, on the basis of the absorption peak, it is possible to detect echinocytes contained in blood.02-14-2013
20130040338DEVICE FOR CAPTURING OBJECT AND METHOD FOR USING THE SAME - Provided is a device for capturing object, wherein a carrier which captures substances to be detected and is made to be dividable into plural portions, is placed with the plural dividable portions arranged in sections. The carrier includes a count analysis carrier and an identification analysis carrier respectively arranged in sections in a first dish half and a second dish half which are obtained by dividing a capturing dish.02-14-2013
20100323391Method of Detecting Microorganisms with a Specimen - This invention concerns a process for preparing reagents intended for a microorganism detection test and notably an infection in humans or animals, wherein the following steps are included: 12-23-2010
20090155839METHOD FOR DETECTING BACTERIAL SPORES - A method of detecting the presence and quantity of bacterial spores, which includes adding an electrophilic alcohol and an acid anhydride to a sample, admixing the sample with a solvent, and analyzing the sample. The sample may be analyzed by injecting the mixture into a gas chromatograph equipped with a mass spectra detector.06-18-2009
20130029373METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING - A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.01-31-2013
20130029372B-CELL ANTIGEN PRESENTING CELL ASSAY - Disclosed herein are methods for diagnosing or predicting B-cell rejection in a subject. In one example, for assessing transplant rejection, the method includes determining an antigen presenting index by comparing uptake of a donor antigen to uptake of a reference antigen in a biological sample obtained from the subject. In another example, for assessing GVHD, the method includes determining an antigen presenting index by comparing uptake of a recipient antigen to uptake of a reference antigen in a biological sample obtained from the subject.01-31-2013
20100136610Methods And Microfluidic Devices For Single Cell Detection Of Escherichia Coli - The present invention features a microfluidic device for detecting 06-03-2010
20100093019SAMPLE HANDLING DEVICE - A device for use in handling a sample, the device including a number of deformable cavities provided on a surface of a substrate, at least one of the cavities being a sample cavity for receiving a sample and a number of fluid channels connecting the cavities such that in use, selective deformation of the cavities causes the sample to be selectively combined with one or more substances.04-15-2010
20120164682METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING - A method for analyzing a blood sample residing within an analysis chamber, which sample has one or more first and second constituents, which constituents are different from one another. The method includes the steps of: a) imaging the sample containing the first and second constituents, which sample is mixed with a colorant that causes the first and second constituents to fluoresce upon exposure to light, and which colorant is also operative to absorb light, the imaging produces image signals representative of fluorescent emissions from the sample and optical density of the sample; b) determining one or more values representative of fluorescent emission from the first and second constituents; c) determining one or more values representative of optical density for the first and second constituents; and d) distinguishing the first constituents from the second constituents using the fluorescent emission representative values and the optical density representative values.06-28-2012
20080286830Analysing Breath Samples for Volatile Organic Compound - The invention describes the use of a biomarker pentylfuran to detect bacterial and/or fungal pathogens. Pentylfuran is released from certain pathogens and detected in the headspace gas of an in vitro culture or in the breath sample of a patient. Pentylfuran is particularly useful in the detection of 11-20-2008
20090123962Chemical Sensing Device - The present application relates to a chemical sensing device for detecting an analyte in a liquid sample containing suspended particles. The device comprises a radiation source adapted to generate electromagnetic radiation, a transducer (05-14-2009
20090042241MICROFLUIDIC DEVICE - Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.02-12-2009
20130089890APPARATUS AND METHOD FOR ANALYZING A CONTAMINATED SURFACE - An apparatus (04-11-2013
20130089889Aggregation Induced Emission Active Cytophilic Fluorescent Bioprobes for Long-Term Cell Tracking - Fluorescent bioprobes comprising fluorogen formed nanoparticles comprising fluorogens with aggregation-induced emission (ATE) properties, which can be used for long-term cell tracking. The fluorogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these ATE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.04-11-2013
20130089888System and Method for Nonlinear Optical Devices - Systems for enhancing the sensitivity of detecting an optical signal using nonlinear optics and method of performing the same. In one embodiment, a single-photon detection system includes an optical amplifier realized in a waveguide, and a photodetector coupled to an output of the optical amplifier. A light detection and ranging system includes the optical amplifier coupled to an optical source and one photodetector. In another embodiment, a photodetection system includes a plurality of optical frequency converters, coupled to an optical source, that sequentially convert a wavelength of photons of the optical source to a final wavelength, and a single-photon photodetector coupled to the optical frequency converters to detect single photons produced by the optical source. In another embodiment, an optical sensor includes an optical pump, and a transducer including an optical ring cavity coupled to the optical pump and configured to utilize optical four-wave mixing to detect an external stimulus.04-11-2013
20130089887Method for Rapid Detection and Identification of micro-colonies using impregnated porous material - This invention describes method for rapid detection and identification of colonies and micro-colonies of microorganisms growing on the surface of certain nutrient agar under certain porous material(s) impregnated by chromogenic and/or flurogenic substrates and supportive substances. Growth in the space between nutrient agar and impregnated porous material allows limiting the negative influence of unfavorable substrates and substances and allows to reveal color or fluorescence spots at early stage of micro-colony growth.04-11-2013
20110003329METHOD FOR NEUTRALIZATION OF ANTIBIOTICS IN A CULTURE MEDIUM - The present invention is directed to a method and means for the neutralization, binding, and/or inactivation of antimicrobials in a test sample. The invention is also directed to a method of detecting the presence of one or more microorganisms in a test sample by culturing the test sample in a culture media comprising one or more primary amine-containing compounds.01-06-2011
20090305336DEVICES AND METHODS FOR SAMPLING MICROBIAL FLORA - Methods, devices and kits for sampling for a microorganism or microbial population on the digits of the extremities of an individual are described. In embodiments of the invention, the device comprises a container, such as a cell culture plate, with a growth medium that is delineated into multiple quadrants, which can be visualized through the growth medium, to aid in the placement of a digit within a designated location on the growth medium.12-10-2009
20130071875METHOD AND SYSTEM FOR THE DETECTIONS OF BIOLOGICAL OBJECTS - The present invention provides a method for identifying biological objects on a substrate; the method comprises: (i) illuminating the substrate carrying the biological objects with a light beam; (ii) acquiring at least one optical image of the illuminated substrate; the light beam comprises a wavelength band, the wavelength band is selected to induce at least one optical aberration in the optical image of the illuminated substrate; the optical aberration is predetermined as characterizing the biological objects; and (iii) processing said at least one optical image to provide a value or a combination of values indicative of presence or absence of said at least one optical aberration; said value or combination of values permitting detection or identification of the biological objects on said substrate. The invention also provides a processing unit, a system and a database for use in connection with the method of the invention.03-21-2013
20110014645METHOD FOR DETERMINING A COMPLETE BLOOD COUNT ON A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.01-20-2011
20130065269FINE PARTICLE MEASURING APPARATUS - A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.03-14-2013
20090233328METHOD FOR READING IMAGES, IN PARTICULAR FOR STUDYING THE DEVELOPMENT OF BIOFILM IN A CULTURE MEDIUM - A method for reading images of a base of a plate provided with at least one well containing a culture medium of micro-organisms includes: i) forming a opaquing layer covering a surface of the culture medium to form a reading base; and ii) imaging, at specific time intervals, the base of the well. using an imaging optical device.09-17-2009
20120115184METHOD FOR THE DETECTION OF MASTITIS AND MILK QUALITY AND MASTITIS SENSOR - A mastitis sensor and a method for detection of mastitis and determination of milk quality in real time (on-line). Methods and apparatuses for the rapid non-invasive determination of the concentration of dissolved molecular oxygen in milked milk are disclosed. Mastitis sensors are disclosed that include a fiberoptic, amperometric or potentiometric device for the determination of oxygen concentration, a device for data acquisition and processing, mastitis indicator and a device that generates a signal for the automatic on-line elimination of substandard milk of infected animals to prevent the pollution of bigger quantities of milk.05-10-2012
20120115181SYSTEMS AND METHODS FOR CEREBROSPINAL FLUID COLLECTION - A test tube insert includes a flange having a width greater than a width of a mouth of a larger test tube into which the insert is to be inserted. The flange defines a first central opening. An annulus extends integrally downward from the flange. The annulus includes a fenestrated wall and a floor defining a second central opening. A small test tube body, smaller than the larger test tube, extends integrally downward from the floor of the annulus with the second central opening forming a mouth of the small test tube body. The test tube insert is disposed in the mouth of the larger test tube. Fluid is transferred through the central opening and the fluid fills the small test tube body of the test tube insert and flows through the fenestrated wall once the small test tube body is full into the larger test tube.05-10-2012
20090215110WATER MONITORING SYSTEMS - The invention relates to a continuous water monitoring system, including components thereof, and a method relating thereto for continuously monitoring, in real-time, a water supply in order to detect contaminants therein. The system employs the use of a live culture of bioluminescent bacteria and suitable light detecting means.08-27-2009
20100068754Density-based cell detection system - The invention provides a low-cost method for identifying and/or measuring a subpopulation of cells in a biological sample, such as a subpopulation of CD4-positive cells in a whole blood sample. In accordance with the invention, one or more cell surface markers are identified on a subpopulation of interest, which collectively are present in higher numbers on the cells of the subpopulation than on cells not in the subpopulation of interest. A probe comprising a binding compound and a density label are combined with the sample and the resulting mixture is centrifuged or sedimented through a density medium in a capillary. Labeled cells of interest pass through the density medium and stack at the bottom of the capillary where they may be visually detected and quantified by the height of the stack.03-18-2010
20080311615METHOD FOR CHIRAL SEPARATION OF LACTIC ACID ENANTIOMERS - A method for the separation and simultaneous determination of urinary D- and L-lactic acid enantiomers by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The chiral separation was done using a chirobiotic teicoplanin aglycone column varying mobile phase parameters such as the acetic acid/triethylamine content and the aqueous/organic ratio.12-18-2008
20120237969Method of Preparing Cells Susceptible to Transmissible Spongiform Encephalopathy and the Creation of TSE Persistently Infected Cells - Disclosed is a method for preparation of transmissible spongiform encephalopathy (TSE) susceptible cells, and such cells may be efficiently used in diagnosis of TSE, etiology studies and development of novel therapeutics, etc.09-20-2012
20120237968Detector and Method for Detection of H. Pylori - An inexpensive, portable, hand held, point-of-care, non-invasive breath-analyzer for the detection of 09-20-2012
20130164776METHOD USED IN A HUMAN OR ANIMAL FAECES SAMPLE PROCESSING SYSTEM, AND A SAMPLE PROCESSING SYSTEM - A sample processing system, and a method in relation thereto, adapted to automatically process a sample, e.g. a faecal sample, prior to the analysis of the sample. The system comprises a sample container for collecting a sample, and a control unit comprising a stored processing recipe that includes parameters to be used to automatically perform the processing of the sample. The control unit is adapted to generate robot control signals, obtained from said processing recipe, to be applied to a robot arranged to move the sample container between different units of the system and to generate control signals to a specific unit when the sample container is about to be treated by that unit. The robot is arranged to automatically move the sample container to: a weighing unit (optional) to determine the weight of the sample; a buffer supply unit to add buffer where the buffer amount can be predefmed or depend on the weight of the sample; a homogenization and mixing unit to homogenize and mix the sample in the buffer, preferably by using sonication by ultrasound. In addition the sample container is preferably moved to: a centrifuge to centrifuge the container in order to produce a clear supernatant; a transfer unit in order to transfer a part of the supernatant to a secondary container, the transferred volume is predefined, or related to the weight of the sample and/or related to the amount of buffer previously added; and if needed, the buffer supply unit adds buffer to the secondary container in order to dilute the sample solution to the desired final concentration.06-27-2013
20130164777Magnetic Flow Cytometry for Individual Cell Detection - The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude06-27-2013
20130017569CULTURE MEDIUM, METHOD FOR CULTURING LISTERIA, AND METHOD FOR DETECTING LISTERIA - There are disclosed a culture media and culture supplements for cultivating microorganisms, including 01-17-2013
20100248299Microbial Detection and Quantification - Suitable dyes are used herein to indicate the presence of microbial contamination by spraying them onto surfaces in the form of an aqueous solution. The dye solution may also be allowed to dry, thus producing the dried residue of an aqueous solution. It's believed that these dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye.09-30-2010
20130023007IDENTIFYING AND MEASURING RETICULOCYTES - Methods and systems for identifying reticulocytes in a blood sample deposited on a substrate include: illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and, for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value.01-24-2013
20120107865DETECTION OF BACTERIA AND FUNGI - A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.05-03-2012
20110287474Cultures and protocols for diagnosis of toxigenic clostridium difficile - Disclosed are culture media, protocols and kits for diagnosis of toxigenic 11-24-2011
20110294158Rapid Detection of Bacteria Using Mass Spectrometric Analysis - Methods for the detection or diagnosis of a bacterial infection or colonisation utilising mass spectrometric analysis are provided. The methods involve short-term enrichment of samples followed by mass spectrometric analysis of biomarker profiles. Also provided are methods for preparing short-term enrichment cultures.12-01-2011
20130189728Identification of pathogens in body fluids - Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.07-25-2013
20110217730SYSTEMS AND METHODS FOR ANALYZING AND MANIPULATING BIOLOGICAL SAMPLES - A system for qualifying cells of a cell sample labeled with a magnetic or magnetizable moiety is provided. The system includes a cell sample holder for holding a cell of the cells and a first cell analyzer which includes a magnetic field source for applying a magnetic field to the cell and a sensor for qualifying and/or quantifying an effect of the magnetic field on the cell.09-08-2011
20080311616Hydrophilic IR Transparent Membrane, Spectroscopic Sample Holder Comprising Same and Method of Using Same - The present invention features hydrophilic IR-transparent porous membranes, particularly hydrophilic IR-transparent porous polyethylene membranes and methods of preparing the hydrophilic membranes by treatment of hydrophobic IR-transparent porous membranes with plasma. The present invention further features spectroscopic sample holders which incorporate the hydrophilic IR-transparent porous membranes and methods of identifying bacteria and other microorganisms in samples by infrared spectroscopy.12-18-2008
20100021960Methods and compositions for pathogen detection using fluorescent polymer sensors - Compositions, methods and related apparatus, as can be used for selective pathogen detection and identification.01-28-2010
20110076716BACTERIA ANALYSIS APPARATUS, BACTERIA ANALYSIS METHOD AND COMPUTER PROGRAM PRODUCT - A bacteria analysis apparatus comprising: a specimen preparation section for preparing a first measurement specimen from a sample by using a first enzyme; a detecting section for detecting bacteria included in the first measurement specimen; and an information processing section for outputting information for supporting determination of kind of bacteria included in the sample on the basis of the detection result of the first measurement specimen. A method and a computer program product is also disclosed.03-31-2011
20100120085Method for separation, characterization and/or identification of microorganisms using mass spectrometry - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for interrogation of the separated microorganism sample by mass spectrometry to produce a mass spectrum of the microorganism and characterizing and/or identifying the microorganism in the sample using the mass spectrum obtained.05-13-2010
20100279338CELL CARRIER CODING - There are provided cell carrier devices for use in cell observation systems, enabling determination of the location of the region under observation by virtue of coding information present in the field of view of the viewing device, even at high magnification. Other embodiments are also disclosed.11-04-2010
20100081166Microbial Detection Assembly - A microbial detection assembly is disClosed which includes a tubular body, a pierceable septum, and a plunger. The tubular body defines a longitudinal axis and has a first end and a second end. The pierceable septum is configured to seal the first end of the tubular body. The plunger head is configured to substantially close the second end of the tubular body. Together, the plunger head, the pierceable septum, and the tubular body define a reservoir dimensioned to receive a medium and a sample substance. Additionally, the plunger head is positioned and is movable within the tubular body in response to changes in pressure within the reservoir.04-01-2010
20120107864MASS SPECTROMETRIC RAPID DETECTION OF SALMONELLA - The invention relates to the detection of specified, flagellated bacteria, particularly 05-03-2012
20100124763Method for detection, characterization and/or identification of microorganisms in a sealed container - The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.05-20-2010
20120034646DETECTION OF H. PYLORI UTILIZING UNLABELED UREA - Disclosed is a method and device for detection of 02-09-2012
20090275075METHODS AND SYSTEMS FOR ENSURING THE SECURITY OF FOOD COMMODITIES - Aspects include claims, systems, and methods for testing food commodities, including without limitation bulk food commodity stores to identify the presence of contaminants. Such contaminants tested for may include biological, chemical, or radio nuclear material. Another aspect is marking and/or tracking food commodity stores that have been certified as either being contaminated or safe. Still another aspect is an automated or automatic system for measuring the level of contamination in a given food commodity store, and for marking and tracking food commodity stores tested for contamination.11-05-2009
20100279339APPARATUS AND METHOD FOR DETECTING BACTERIAL GROWTH BENEATH A WOUND DRESSING - An apparatus and method are provided for visually monitoring, detecting, and/or determining the presence, absence, and/or growth of harmful or potentially harmful bacterial microorganisms beneath a wound dressing, in one example used to cover an indwelling central venous catheter or other catheter. A bacteria detection apparatus includes a barrier membrane, a permeable membrane for placement proximate a wound or a catheter insertion site, and an indicator between the barrier membrane and the permeable membrane for indicating the presence of bacteria proximate the permeable membrane. A method of using a bacterial growth detection apparatus is also provided.11-04-2010
20100136609Method for separation, characterization and/or identification of microorganisms using raman spectroscopy - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.06-03-2010
20110201049COMPOSITIONS FOR THE DETECTION OF MICROBIAL CONTAMINANTS - The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.08-18-2011
20100136608Multiple Filter Array Assay - The present invention relates to a method and device for the assay and determination of the concentration of organisms or other filterable constituents of a fluid test sample. The method includes the steps of exposing, processing, and analyzing a liquefied sample in a filter tray apparatus. Each filter element (FE) filters a volume of liquid from a common fluid test sample. Excess unfiltered liquid and filtrate is removed from the filter tray apparatus. Each filter element contained in the filter tray apparatus is exposed to culture medium and/or appropriate testing reagents introduced to the apparatus to generate an indicator of the filterable constituent. Final examination is conducted on the filter tray apparatus for the indication of the presence and/or absence of filtered constituents in the multiple filter elements. The presence/absence information defines the concentration of the filterable constituent in the fluid test sample.06-03-2010
20110207168Fiber-Based Biosensors for Use in Detecting the Presence of a Biologically Active Substance - Disclosed are fibers comprising one or more electrostatically attached substrates that can be used to determine the presence of a biologically active substance. Further disclosed are substrates comprising the fibers, articles of manufacture comprising the fibers and/or substrates, and methods for detecting the presence of a biologically active substance.08-25-2011
20100136607SENSOR DEVICE - The invention relates to a sensor device for detecting CO06-03-2010
20080286831SAMPLE COLLECTOR AND TESTER - Provided are devices and methods for testing solid, semi-solid, or liquid animal or environmental specimens. The multi-part device comprises a breakable seal that separates a specimen treatment chamber from a testing chamber.11-20-2008
20090004690COMPOSITION AND METHODS FOR MAKING 5, 10, 15, 20-TETRAKIS (CARBOXYPHENYL) PORPHINE - Presented is a method to detect precancerous states in mammalian cell and tissue samples comprising incubating a sample with solubilized 5, 10, 15, 20-tetrakis (carboxyphenyl)porphine (TCPP), measuring TCPP fluorescence in the sample, and categorizing the sample as non-cancerous, precancerous or cancerous based on TCPP fluorescence, as correlated with the respective states of the cells. In conjunction with the method a detection, a novel and more efficient method of solubilizing TCPP is presented, as well as a composition comprising TCPP solubilized by this method.01-01-2009
20100143964COMPOSITIONS AND METHODS FOR CONCENTRATING AND DEPLETING MICROORGANISMS - Methods for concentrating microorganisms in a liquid sample or depleting microorganisms therefrom, utilizing polymeric compounds having affinity to microbial cells that are composed of a plurality of positively charged amino acid residues and two or more hydrophobic moieties are disclosed. Also disclosed are devices for concentrating and methods for detection and identification microorganisms in a liquid sample.06-10-2010
20110269178FLUORESCENT CDK INHIBITORS FOR TREATMENT OF CANCER - Disclosed are molecules and their synthesis. The fluorescent moiety also facilitates screening, tracking, and pharmacodynamic studies of the drug in a biological system both in vitro and in vivo.11-03-2011
20110269177ANTIMICROBIAL AGENT AND METHOD FOR THE PRODUCTION THEREOF - Provided are antimicrobial agents produced from 11-03-2011
20090029408Methods for Characterizing Cell Proximity - One aspect of the invention relates to a method of identifying cell adjacency in a coculture of cells. The method includes generating a first set of image data of the coculture of cells, the first set of image data comprising nuclear position data for each imaged cell; generating a second set of image data of at least a subset of the coculture of cells, the second set of image data comprising cell identifier data associated with a first cell type; generating a third set of image data of at least a subset of the coculture of cells, the third set of image data comprising cell identifier data associated with a second cell type; and identifying a group of cells in response to the nuclear position data, wherein each cell having the first cell type in the group is adjacent another cell in the group having the second cell type.01-29-2009
20090162891Effective Monitoring System or Anthrax, Smallpox, or Other Pathogens - A device and method for detecting anthrax or other pathogens are disclosed. Individual self-contained monitoring devices of a monitoring system can be portable or stationary (e.g. installed in air ducts or plumbing of buildings) and can be part of a network of devices. Monitoring devices may be used for the detection of a variety of airborne or surface pathogens, including but not limited to anthrax, smallpox, and 06-25-2009
20090325221Temporary Tattoo Decals for Detecting the Presence of an Analyte - Temporary tattoos to be applied to the skin of a wearer for the detection of an analyte are generally disclosed. The temporary tattoo can be applied to the skin via a temporary tattoo decal. The temporary tattoo can indicate the presence of an analyte by displaying a certain spectral response (e.g., color change) in the presence of a targeted analyte. This change in color can indicate to the wearer and/or caregiver the presence of the targeted analyte in real-time.12-31-2009
20110223631Detection of microorganisms with a fluorescence-based device - A device and method for detecting by fluorescence microbial growth from sample substances are disclosed. For example, a method for the detection of visible-band fluorescence signals generated by at least one fluorescing compound excited by ultraviolet energy, comprising exciting said at least one fluorescing compound with ultraviolet energy emitted from a light-emitting diode comprising wavelengths below 09-15-2011
20110143391METHOD FOR TYPING AND IDENTIFICATION OF MICRO-ORGANISMS - The invention relates to a method for typing or identification of a micro-organism using vibrational spectroscopy, characterised in that, differences in one or more vibrational spectroscopic signal contributions from one or more bleachable components between different spectra obtained of a sample of said micro-organism, and/or between spectra of different samples of said micro-organism, and/or between spectra of samples of different micro-organisms, are substantially eliminated as a source of signal variance in a dataset of said spectra, said bleachable components being molecules possessing vibrational spectral bands that upon exposure to light show a reduction in signal intensity.06-16-2011
20110229929Arsenical Fluorescent Agents and Assays - The invention provides methods and compositions for labeling dithiol-containing analytes.09-22-2011
20090239257METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING - A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.09-24-2009
20130137134METHOD AND SYSTEM FOR DETECTING AND MONITORING HEMATOLOGICAL CANCER - A method for diagnosis of a hematological malignancy of a subject is provided. The method comprises obtaining a second derivative of an infrared (IR) spectrum of a population of mononuclear cells by analyzing the population of mononuclear cells by infrared spectroscopy, and based on the second derivative of the infrared spectrum, generating an output indicative of the presence of a hematological malignancy. Other applications are also described.05-30-2013
20130137135COMPOSITIONS AND METHODS FOR LEUKOCYTE DIFFERENTIAL COUNTING - The present invention provide compositions and methods for classifying leukocytes in a leukocyte population using fluorescence detection. The methods include contacting a leukocyte population in a sample having one or more leukocyte types with a diagnostic composition, exciting the leukocyte population with a light source; and measuring emitted light from each of the one or more leukocyte types to classify the leukocyte population.05-30-2013
20130122537INTEGRATED OPTICAL SENSOR - Optical sensors for use in examining and analyzing a fluid material are described. The optical sensors include a channel configured to transport a fluid material to be examined and analyzed, a first waveguide positioned adjacent to the channel and configured to guide radiation through the channel and a first tunable grating defined on the first waveguide and configured to variably define a cavity for selecting a first wavelength of the radiation.05-16-2013
20130122538Dynamic Light Scattering for in vitro Testing of Bodily Fluids - A method of diagnosing a pathological condition by detecting microparticles in a sample of bodily fluid using dynamic light scattering (DLS) is disclosed. The detection of microparticles in the bodily fluid by DLS may be used as an indicator of existing disease, to evaluate a risk of disease, as well, as to monitor the efficacy of a treatment for disease.05-16-2013
20130122539MICROSIEVE FOR CELLS AND PARTICLES FILTRATION - It is disclosed a microsieve comprising two layers, wherein the first layer is a membrane layer having a plurality of micropores contained therein and a thickness of about 10 μm to about 100 μm, and the second layer is a membrane support layer having a plurality of openings contained therein and a thickness of about 100 μm to about 500 μm, wherein the openings are larger in diameter than the micropores, and wherein at least one of the membrane layer or membrane support layer is formed of a SU-8 photoresist material.05-16-2013
20110177547Particle Sorting Using Fluid Streams - A fluidic device includes an arrangement of channels for introducing a sample containing particles of interest into a processing chamber. The chamber is in fluid communication with collecting channels via low-flow connection channels. Particles in the sample may be observed and diverted from the processing chamber by application of a motive force such as optical trapping into a collection channel. Once in the collection channel, particles can be collected, including by trapping in a porous matrix.07-21-2011
20100184123Gas Exchange Detection Method and Device - The invention relates to a gas exchange detection method and device particularly, but not exclusively, for rapid detection of the presence of micro-organisms, the effect of an additional substance upon a micro-organism and/or the rate at which oxygen is consumed, in solutions, aqueous emulsions and/or suspensions.07-22-2010
20100216183MICROBIAL DETECTION APPARATUS, MICROBIAL DETECTION METHOD, AND SAMPLE CONTAINER USED THEREIN - The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.08-26-2010
20100227360SALMONELLA SELECTIVE ENRICHMENT MEDIUM CONTAINING TETRATHIONATE AND MAGNESIUM SALT - The invention relates to a salmonella selective enrichment medium containing tetrathionate or a salt thereof and at least one type of magnesium salt and to a method for detecting salmonella in a sample by using said selective enrichment medium.09-09-2010
20100227359System and method for detection of substances - A system, a method, and apparatus for determining the presence of an agent in a sample. The system includes a sampler for collecting the sample, and an attachment means for attaching the sampler to the sample. In addition, the system includes a carrier, wherein the sampler with the sample is substantially aligned with a portion of the carrier. The system also includes an analyzing device which is substantially aligned with the carrier and the sample. Finally, the system includes a chemistry for identifying an indicator of the agent in the sample as determined by the analyzing device.09-09-2010
20100240092DETECTION METHOD AND DETECTION APPARATUS - Provided is a method of adjusting detection sensitivity in detecting a sample in a channel. A detection method of optically detecting a sample includes: forming a multilayer flow, in which at least one of layers of the multilayer flow contains the sample; introducing light into at least one of layers of the multilayer flow; and detecting a signal generated from the multilayer flow in response to the introduced light, to detect the sample. The sample includes one of a chemical substance, a molecule, a cell, a particle, and a mixture thereof. At least one of fluids included in the multilayer flow has a refractive index different from a refractive index of another one of the fluids.09-23-2010
20100240091BIOCHEMICAL TEST FOR CONFIRMING THE PRESENCE OF L. MONOCYTOGENES - The invention relates to a biochemical test for confirming the presence of 09-23-2010
20130217064Integrated cultivation and measurement device for label-free detection and classification of cellular alterations, in particular for generation and characterisation of cell-spheroids, components and uses thereof - The invention relates to an integrated cultivation and measurement device for label-free detection and classification of cellular alterations, in particular for generation and characterisation of cell-spheroids and monitoring the condition of the cell-spheroids in real time, comprising a) a mounting device for a cultivation chamber plate, b) an amplifier board linked with the contacts for the microelectrodes in the mounting device, c) a rotary shaker, on which the amplifier board and the mounting device for the cultivation chamber plate are placed, and d) a control unit, that is linked with the amplifier board and the rotary shaker, wherein the control unit allows recording, analyzing of data and controlling of the movement of the rotary shaker. The cultivation chamber plate has several culture reservoirs, wherein the bottom of each culture reservoir forms a microcavity and each microcavity features microelectrodes on the microcavity walls. The mounting device has contacts for the microelectrodes. The invention advantageously allows the automated generation, cultivation and characterisation of spheroids, as well as the cultivation and characterisation of tissue probes.08-22-2013
20100255529DEVICE AND METHOD FOR MICROBIOLOGICAL ANALYSIS OF BIOLOGICAL SAMPLES - The device for microbiological analyses on samples of body fluids comprising: an incubation area for containers containing said samples; an analyzer for analyzing the inner atmosphere of said containers; a sorting system to sort the containers according to the carbon dioxide content detected by said analyzer.10-07-2010
20090258385METHOD OF ANALYZING CELLS BY FLOW CYTOMETRY, USE OF AN ANTIOXIDANT AND/OR FREE RADICAL-SCAVENGING AGENT IN SUCH A METHOD AND INCUBATION BUFFER COMPRISING AN ANTIOXIDANT AND/OR FREE RADICAL-SCAVENGING AGENT - The invention relates to a method of analyzing cells by flow cytometry and the use of an antioxidant and/or free radical-scavenging agent in such a method. It also relates to an incubation buffer comprising an antioxidant and/or free radical-scavenging agent. The method of the invention comprises a step for labeling cells in a labeling solution comprising an incubation buffer and the labeling antibody or antibodies conjugated with at least one fluorochrome which is an APC tandem and an antioxidant and/or free radical-scavenging agent. The invention finds application in the field of the analysis of cells by flow cytometry, in particular.10-15-2009
20090170151FLOW-THROUGH CELL AND METHOD OF USE - There is disclosed a flow-through cell comprising a substrate defining a channel, having an inlet and an outlet, at least a portion of the substrate being light-permeable to allow particles within at least a portion of the channel between the inlet and the outlet to be optically detected through the substrate, wherein the flow-through cell comprises liquid-permeable particle retaining means located downstream of the at least a portion of the channel where particles can be optically detected, for allowing the flow of a liquid sample through the channel from the inlet to the outlet while retaining particles from the liquid sample whose dimensions exceed threshold dimensions within the channel, where they can be optically detected. The flow-through cell is particularly applicable to the detection of micro-organisms in drinking water.07-02-2009
20100304429METHODS AND APPARATUS FOR SORTING CELLS USING AN OPTICAL SWITCH IN A MICROFLUIDIC CHANNEL NETWORK - Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on an optical switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format.12-02-2010
20110111451Gross Hematuria Evaluator and Methods for Making and Using Same - A device for evaluating the blood content in a plurality of media may include a display having a plurality of columns, each column having a plurality of color-block rows, where the colors are created by diluting blood in the respective medium to obtain a color, and then substantially matching that color with a row in the column. Additionally or alternatively, the columns may represent blood diluted in the same sample, but observed at different times. The display may include gaps between columns to accept a catheter in which the sample to be analyzed is placed and a legend identifying the medium and blood dilution for each column and row, as well as the time of aging, if any, for a column.05-12-2011
20110244510Filtration method for detecting microbial contamination - Disclosed is a convenient sample preparation method for a medium suspected of containing contaminants, the method comprising a) passing a known volume of said medium through a filter from an influent side to an effluent side thereby concentrating the contaminants on the influent side of the filter, b) contacting the influent side of the filter with a liquid vehicle containing at least one substrate that through interaction with the contaminants each produces a detectable moiety, c) and allowing the substrate to interact with the contaminants on the influent side of the filter for a period of time, which is sufficient to allow the detectable moiety to be detected in the liquid vehicle. The method may further comprise a detection step, where the amount of detectable is determined in the liquid vehicle, preferably after the liquid vehicle has been separated from the contaminant, e.g. by passing the liquid vehicle through the filter and performing a measurement on the contaminant free liquid vehicle. Also disclosed is a kit for exercising the inventive method.10-06-2011
20100047853Ex Vivo Culture, Proliferation and Expansion of Intestinal Epithelium - Methods are provided for long term culture of mammalian intestinal cells. Cultures are initiated with fragments of mammalian intestinal tissue, which are then maintained embedded in a gel substrate that provides an air-liquid interface. Intestinal epithelium in cultures of the invention can be continuously grown for extended periods of time. Mammalian intestinal cells cultured by the methods of the invention recapitulate features of intestinal growth in vivo.02-25-2010
20100221771Serum-Free Growth Medium for Acholeplasma laidlawii and Methods for Retention Testing Sterilizing Grade Filters - A method for retention testing sterilizing grade filters comprises: a) providing a stock of 09-02-2010
20110076715METHOD OF SINGULATING EMBRYOS - A method of singulating embryos is provided. The method includes providing a plurality of embryos (03-31-2011
20100047851Methods and compositions for the detection of microbial contaminants - The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.02-25-2010
20110086383APPARATUS AND METHODS FOR TREATING ALLOGRAFT PRODUCTS - Apparatus for treating allografts, having a sonication tank configured to transmit ultrasonic energy to the interior of the tank; a treatment canister rotatably positioned in said sonication tank, and configured to receive allografts therein; and a treatment fluid source in fluid communication with said treatment canister. Methods of treating allografts and methods for determining microbial contamination using the apparatus.04-14-2011
20100068755Method and system for detection and/or characterization of a biological particle in a sample - The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.03-18-2010
20110076714Method for the presence detection of microorganisms in a bioreactor, bioreactor and arrangement - The invention relates to a method for the presence detection of microorganisms in a bioreactor (B), to a bioreactor (B), and to an arrangement for performing the detection method. In order to reduce the staffing expense during the detection analysis of the presence of undesirable microorganisms in a bioreactor (B) after the bioreactor (B) has been cleaned, the detection method is carried out for microorganisms present in a bioreactor (B) such that a) a nutrient medium is selectively applied to defined areas of the bioreactor (B) in a controlled manner by a control unit (SE), b) an incubation having a defined time is performed in the bioreactor (B), c) after the incubation has been completed, the nutrient medium is removed from the defined areas in the bioreactor (B) and fed to an analysis unit (AE), in which the presence of microorganisms or decomposition products is determined, d) the analysis result determined in c) is then sent to the control unit (SE), which based on the results of the analysis selects a suitable process step from the predefined process steps for the further operation of the bioreactor (B).03-31-2011
20110081676RAPID DETECTION OF VIABLE BACTERIA SYSTEM AND METHOD - An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample.04-07-2011
20120149057METHOD FOR PROGNOSING RESPONSE TO CANCER THERAPY WITH 5, 10, 15, 20-TETRAKIS (CARBOXYPHENYL) PORPHINE - Presented is a method of prognosing a patient's response to a cancer therapy wherein prior to the therapy contacting a sample of cells from the patient's tissue or organ being treated for the cancer with a solution of TCPP to permit binding of the TCPP to components of the abnormal dysplastic or carcinomic cells, if any are present; detecting TCPP fluorescence in the sample, the presence of TCPP fluorescence being indicative that the sample contains dysplastic or carcinomic cells; at intervals during the therapy and subsequent to the therapy performing steps a-c on another sample of cells from the patient's tissue or organ being treated for the cancer; and determining if the percentage of abnormal pre-cancerous cells in the samples tested during and subsequent to the therapy are reduced as compared with the sample tested prior to the therapy, the reduction being prognostic of the patients response to the cancer therapy.06-14-2012
20120149056METHOD AND REAGENTS FOR DETECTING THE PRESENCE OR ABSENCE OF A TARGET SUBSTANCE IN A TEST SAMPLE - Reagent mixtures and testing procedures for detecting the presence or absence of various target substances such as aureus; vancomycin-resistant enterrococcus; Group B 06-14-2012
20120034647METHOD AND APPARATUS FOR AUTOMATED WHOLE BLOOD SAMPLE ANALYSES FROM MICROSCOPY IMAGES - A method and apparatus for identifying one or more target constituents (e.g., white blood cells) within a biological sample is provided. The method includes the steps of: a) adding at least one colorant to the sample; b) disposing the sample into a chamber defined by at least one transparent panel; c) creating at least one image of the sample quiescently residing within the chamber; d) identifying target constituents within the sample image; e) quantitatively analyzing at least some of the identified target constituents within the image relative to one or more predetermined quantitatively determinable features; and f) identifying at least one type of target constituent within the identified target constituents using the quantitatively determinable features.02-09-2012
20100297691DEVICES AND PROCESSES FOR COLLECTING AND CONCENTRATING SAMPLES FOR MICROBIOLOGICAL ANALYSIS - The present invention refers to manual devices for the collecting and concentrating liquid samples for microbiological analysis and their respective methods of use. The manual devices comprise a body which contains a sample, a removable support, a microporous membrane, and a plunger. The present invention is also directed to methods of collecting and concentrating of liquid samples onto a microporous membrane for micro-biological analysis.11-25-2010
20100311108System and method for agitation of multiple specimen containers - A system is disclosed for agitating multiple specimen containers each containing a sample and a growth medium. The system includes a plurality of racks. The racks have a plurality of receptacles, each of which is adapted for holding a specimen container. The racks include a first rack oriented in a first direction and a second rack oriented in a second direction different from the first direction. The system also includes a rotating turret having a frame holding the racks. The frame is rotatable about an axis, wherein the rotating turret operates to rotate the racks about the axis to an access position, e.g., where a user may access the specimen containers. The system also includes an agitation assembly coupled to the racks. The agitation assembly rocks the racks back and forth thereby agitating specimen containers held by the racks. In one configuration, the system is incorporated into an automated instrument detecting whether a microbiological agent is present in the specimen container. In another configuration, the system is incorporated into an automated instrument identifying or characterizing the microbiological agent.12-09-2010
20100297690Detection of salmonella lactose+ - The invention relates to a medium for the detection and/or identification of 11-25-2010
20090023179Reagent, Medium, and Method for Detection of Alicyclobacilus Acidoterrestris - To supply the reagent, medium, and method for detection of 01-22-2009
20110151501MODULAR SYSTEM AND METHOD FOR DETECTING MICROORGANISMS - A modular automated system (06-23-2011
20090317860STANDARD MATERIAL FOR A PARTICLE ANALYZER - A standard material that is used for judging an abnormal portion in a particle analyzer is described. The standard material comprises first standard particles to be fluorescence-stained by a fluorescence-staining treatment and second standard particles that have preliminarily contained a fluorescence dye. A method and an analyzer that can judge an abnormal portion in a particle analyzer by using such a standard material are also described.12-24-2009
20090311741ENVIRONMENTAL EVALUATION INSTALLATION AND ENVIRONMENTAL EVALUATION METHOD - An environmental evaluation installation including an evaluation chamber 12-17-2009
20080293094Novel Enzymatic Substrates Derived from Phenoxazinone and Their Use as Developer in Detection of Microorganisms with Peptidase Activity - The invention concerns novel enzymatic substrates of the general formula below:11-27-2008
20110256584CULTURE MEDIUM FOR CULTIVATION AND IDENTIFICATION OF BACTERIA OF GENUS PECTINATUS AND METHOD FOR TAKING SWAB SAMPLES - The present disclosure relates to a culture medium for cultivation and identification of bacteria of genus 10-20-2011
20080254502Method and Apparatus for Detection of Bioaerosols - A method and apparatus for evaluating a bioaerosol sample is provided which includes detecting frequency and/or time resolution factors that allow discriminate between a plurality of signals emitted by the bioaerosol to selectively detect biological materials contained in the bioaerosol sample from materials of non-biological origin and potentially associated with a pathogenic bioaerosol.10-16-2008
20110020860FECAL EXAMINATION METHOD AND COLLECTION CONTAINER - A method for conducting a fecal examination includes providing a collection spoon and a collection container comprising a transparent body having a cap which can be removed to permit fecal matter to be received in the collection container with the spoon to be mixed with a flotation material to aid in separating the fecal matter and ova or eggs of parasites that may be contained in the fecal matter and then dispensed through a filter mounted in the cap of the collection container to a test tube and a cover slip on the test tube for examination of the filtered material for the presence of the ova of parasites.01-27-2011
20110053212BLOOD ANALYZER, BLOOD ANALYSIS METHOD, HEMOLYTIC AGENT AND STAINING AGENT - This blood analyzer includes a sample preparation portion preparing a first measurement sample containing a blood sample and a hemolytic agent and a second measurement sample containing the blood sample, the same hemolytic agent as the hemolytic agent and a staining agent and a control portion classifying white blood cells in the first measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of fluorescent information and two types of scattered light information generated by a light information generation portion and classifying blood cells in the second measurement sample into at least malaria-infected red blood cells and others on the basis of fluorescent information and scattered light information generated by the light information generation portion.03-03-2011
20110053211PROCESS AND DEVICE FOR DETERMINING THE CONDITION OF BIOLOGICAL MATERIAL, IN PARTICULAR FOOD - The invention relates to a process for determining—remotely and without taking samples—the condition of biological material, in particular food, as well as a device for performing this process. Radiation emission is thus induced with coherent beams in the material to be examined and is directly measured, whereby the measured values are compared to a nominal or boundary value. To this end, the device has a radiation source for emitting coherent beams, a detector for determining the induced radiation emission, and a control device, whereby the control device contains a microcomputer unit for comparison of the determined radiation emission with the nominal and boundary values that are stored in the memory.03-03-2011
20110027822SYSTEMIC LUPUS ERYTHEMATOSUS - This document relates to methods and materials involved in diagnosing SLE. For example, this document relates to methods and materials involved in diagnosing SLE, diagnosing severe SLE, and assessing a mammal's susceptibility to develop severe SLE. For example, this document provides nucleic acid arrays that can be used to diagnose SLE in a mammal. Such arrays can allow clinicians to diagnose SLE based on a simultaneous determination of the expression levels of many genes that are differentially expressed in SLE patients as compared to healthy controls. In addition, methods and materials for assessing SLE activity, determining the likelihood of experiencing active SLE, and detecting SLE treatment effectiveness are provided herein.02-03-2011
20100285522Versatile Multichannel Capillary Biosensor System - The multichannel capillary biosensor system includes a selecting valve system for selection of the sample or a reagent. The selecting valve system is connected to a second valve system accommodating a sensing channel and a first bypass channel. The second valve system is connected to a directing valve system having at least two positions, with a first position being connected to a second bypass channel and a second position being connected to a detector for detecting the analyte. The device has a pump connected at one end to the second bypass channel and the detector and at a second end to a waste vessel. The device also has a control system for controlling the device. The analyte can be selected from the group of microorganisms, macromolecules, and small molecules.11-11-2010
20110117595Testing Unit and Method for Testing a Sterilization Packaging Unit - A testing unit and a method of testing a sterilization packaging unit for microbial contamination of sterilized objects after they have been sterilized are disclosed. The testing unit includes a dry matrix for accommodating a nutrient medium and a receptacle containing a liquid. By applying the liquid onto the matrix, the matrix is provided with a nutrient medium. In the method, the testing unit is inserted into the sterilization packaging unit which is sterilized and stored. At a desired point in time, the receptacle is opened in order to produce the nutrient medium in the matrix. After a possible multiplication of microbes on the matrix, an observation of microbes takes place. The invention also relates to a sterilization packaging unit which has a rigid outer casing and is suitable for the application of the method.05-19-2011
20100323392Metering Technique for Lateral Flow Assay Devices - A diagnostic test kit for detecting an analyte residing in a test sample is provided. The kit employs a lateral flow device that contains a membrane. A metering channel is formed in the membrane that is capable of delivering a controlled volume of the test sample to a detection zone upon initiation of the assay. Such a metering channel is particularly effective for embodiments in which the test sample has a relatively low volume, such as less than about 100 microliters, in some embodiments from about 0.5 to about 25 microliters, and in some embodiments, from about 1 to about 10 microliters. For example, whole blood drops obtained from patients with a lancet typically have a volume of less than about 3 microliters. Despite their low volume, the present inventors have discovered that the blood drops may still be accurately analyzed for the presence of an analyte using lateral flow detection techniques.12-23-2010
20100330613METHODS FOR DETERMINING EFFICACY OF CHEMOTHERAPEUTIC AGENTS - An improved system for screening a multiple of candidate therapeutic or chemotherapeutic agents for efficacy as to a specific patient, in which a tissue sample from the patient is harvested, cultured and separately exposed to a plurality of treatments and/or therapeutic agents for the purpose of objectively identifying the best treatment or agent for the particular patient. Specific method innovations such as tissue sample preparation techniques render this method practically as well as theoretically useful. By subjecting uniform samples of cells to a wide variety of active agents (and concentrations thereof), the most promising agent and concentration for treatment of a particular patient can be determined.12-30-2010
20110256583METHOD AND MEDIUM FOR ACCELERATING TARGET ANALYTE GROWTH IN A TEST SAMPLE - A reagent mixture and testing procedure for accelerating mixing of the formulation with a specimen sample being tested by effervescing when hydrolized, thereby causing an internal “stirring” of the reagent and the specimen sample. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, environmental swabs, foodstuffs, and water, to mention a few.10-20-2011
20100159505REAL-TIME MONITORING OF AGE PIGMENTS AND FACTORS RELATING TO TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES AND APPARATUS - A fluorescent spectroscopic method and apparatus for real time direct detection of transmissible spongiform encephalopathies in central nervous system tissue by monitoring the fluorescence of intrinsic markers in the tissue by illuminating the tissue with UV or visible light having an appropriate wavelength, and the resulting emission spectra is detected and examined in the region from 350 to 650 nm. A higher intensity in this region is indicative of infected tissue. The apparatus and method would not interfere with existing slaughterhouse line speeds or procedures, and could be used on live animals.06-24-2010
20100159504Integrated Microbial Collector - A system for real-time sizing of fluid-borne particles is disclosed. The system further determines, in real time, whether the detected particles are biological or non-biological. As the fluid is being tested, it is exposed to a microbe collection filter which is cultured to determine the type of microbes present in the fluid being tested.06-24-2010
20100068756METHOD AND APPARATUS FOR DETECTING AND QUANTIFYING BACTERIAL SPORES ON A SURFACE - A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: bacterial spores are transferred from a place of origin to a test surface, the test surface comprises lanthanide ions. Aromatic molecules are released from the bacterial spores; a complex of the lanthanide ions and aromatic molecules is formed on the test surface, the complex is excited to generate a characteristic luminescence on the test surface; the luminescence on the test surface is detected and quantified.03-18-2010
20090197298METHOD FOR DETECTING MICROORGANISMS IN A BIOLOGICAL SAMPLE - This invention relates to a method for detecting at least one specific microorganism in a biological sample, said method comprising the following steps: a) culturing said biological sample in a culture medium, b) subjecting said culture medium to at least two radiations each presenting a specific wavelength, c) obtaining at least two different images of said culture medium using at least two reading systems, d) combining said at least two different images to produce at least one combined artificial image, and e) analyzing said combined artificial image to detect the presence of said at least one specific microorganism in the biological sample. The invention also relates to a device for application of the detection method according to this invention.08-06-2009
20090176268 Solid Nutrient Media Useful For Isolating And Identifying Alkaliphilic Bacteria - The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. Te media composition consists of 5-15 g of carbon source, 2.5-10 g of peptone, 2.5-10 g of yeast extract, 0.5-1.5 g of dipotassium hydrogen phosphate; 0.1-0.5 g of magnesium sulphate heptahydrate, 30 μl-4 ml of super saturated solution of sodium hydroxide, 5-20 g of potassium chloride and 10-30 g of κ-carrageenan in one litre of distilled water. The potassium salt in combination with κ-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria.07-09-2009
20120309048DIAGNOSTIC APPARATUS - A diagnostic apparatus (12-06-2012
20120309047Color Changing Polymer Films for Detecting Chemical and Biological Targets - A sensor system, and a method of detecting a target analyte, comprises a chemically functionalized block copolymer, and a target analyte. The block copolymer exhibits a color change in the visible spectrum upon exposure to the target analyte.12-06-2012
20110262960MYCOBACTERIA CULTURE MEDIUM AND METHOD INCLUDING MYCOBACTERIA OF MYCOBACTERIUM TUBERCULOSIS COMPLEX - The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the 10-27-2011
20100021959High Throughput Screening Method for Assessing Heterogeneity of Microorganisms - The invention relates to the field of microbiology. Provided is a method which is particularly powerful for High Throughput Screening (HTS) purposes. More specific a high throughput method for determining heterogeneity or interactions of microorganisms is provided.01-28-2010
20080305514METHOD FOR DETECTING MICROBES - The present invention relates to methods for detecting microbes in a sample comprising filtering the sample through a fluid-permeable surface, contacting the surface with a viability stain, scanning the surface for viability stain to form a first scan, contacting the surface with a nucleic acid stain, scanning the surface for nucleic acid stain to form a second scan, and comparing said first scan and said second scan.12-11-2008
20120231492SENSOR FOR DETECTING MICROORGANISMS AND CORRESPONDING PROCESS - Microbial fuel cells generate an electrical signal when microbes enter the cells through a semipermeable membrane. By reading and analyzing the signal from one or more such fuel cells can indicate infection in people or animals, indicate pathogens growing in food or show mold growth. Insofar as different microbes have specific metabolisms, the signal may be used to determine which microbe is present.09-13-2012
20110306087APPARATUS TO ANALYZE A BIOLOGICAL SAMPLE - An apparatus to analyze a biological sample contained in a container, comprising an examining device able to perform an optical measurement on the biological sample, based on light-scattering technology, at least of the bacterial growth in the biological sample. The examining device is provided with emitter means able to emit a first light beam toward the container of the biological sample and sensor means able to detect at least a light beam diffused from the container of the biological sample and to transmit a relative signal, correlated to the light beam diffused, to a control unit which is able to process, directly or indirectly, the signal in order to verify at least the possible bacterial growth in the biological sample. The container is disposed along a determinate lying plane and comprises at least a containing micro-element able to contain at least a part of the biological sample and inside which a liquid culture ground is provided so as to allow bacterial growth in the biological sample. The emitter means and sensor means are able to be located on each occasion in correspondence with the containing micro-element. The sensor means comprise first sensor means disposed on the same side of the emitter means with respect to the lying plane, and second sensor means disposed on the opposite side of the emitter means with respect to the lying plane. The first sensor means are able to detect a back-scattering radiation coming from the determinate containing micro-element. The second sensor means are able to detect a forward-scattering radiation coming from the determinate containing micro-element, from which back-scattering radiation and forward-scattering radiation derive respective first and second signals transmitted to the control unit.12-15-2011
20110306086CELL SEPARATING METHOD - Provided is a cell separating method which suppresses physical damage to cells without using an apparatus having a complicated structure, and enables the cell separation performed by a quantitative analysis and a multi-parametric analysis and the cell separation using Hoechst staining or the like as an index.12-15-2011
20110306085COMPOSITIONS AND METHODS FOR DEGRADING LIGNOCELLULOSIC BIOMASS - The present invention relates to compositions and methods of producing bioenergy products and metabolites of industrial interest from lignocellulosic biomass. More specifically, the invention describes the identification, characterization and isolation of novel bacteria having the remarkable ability to transform lignocellulosic biomass into fermentable sugars and, even more remarkably, into bioenergy products and metabolites. The invention also discloses a method to isolate such bacteria, compositions comprising such bacteria, and their uses for the modification of lignocellulosic biomass, with a view to producing bioenergy.12-15-2011
20100047852SELECTIVE CULTURE MEDIUM - The present invention discloses a selective culture medium for detecting MRSA and selective drug or multidrug resistant microorganisms where the expression of said (multi)drug resistance is by virtue of the production by said microorganisms of β-lactamase enzymes. The medium comprises: (i) a broad spectrum antibiotic capable of selectively inhibiting growth/replication of (a) organisms that do not produce β-lactamase enzymes, (b) organisms that while producing β-lactamase do not exhibit an ESBL or AmpC phenotype and (c) Methicillin-susceptible 02-25-2010
20100093020SOLVENT TOLERANT MICROORGANISMS AND METHODS OF ISOLATION - bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerant 04-15-2010
20100129857Methods for the isolation and identification of microorganisms - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.05-27-2010
20110111452METHOD AND KIT FOR DIAGNOSIS OF MALE FERTILITY - A method and kit are disclosed for the diagnosis of male fertility.05-12-2011
20080220466In Vitro Techniques For Use With Stem Cells - A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g/ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.09-11-2008
20120301916Growth Medium For The Detection Of Microorganisms By Fluorescence Allying A Fluorogenic Substrate And A PH-Sensitive Fluorophore - The invention relates to a growth medium allying a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4-methylumbelliferone (4-MU) and derivatives of fluorescein. This growth medium is used for the detection by fluorescence of microorganisms by coupling the fluorescence measurements relating to the pH-sensitive fluorophor and the fluorescence measurements relating to the activation of the fluorogenic substrate(s) by the microorganisms.11-29-2012
20120064566MULTIPLE PHASE FLOW SYSTEM FOR DETECTING AND ISOLATING SUBSTANCES - A multiple flow system and method for detecting substances in a fluid is provided. More specifically, a first fluid tube containing a first fluid and a second fluid tube containing a second fluid are coupled to a common fluid tube via a connector, such that alternating discrete compartments of the first fluid and the second fluid flow through the common fluid tube. The first and second fluids are immiscible. A substance detector, having a flow chamber with an internal wall, is coupled to the common fluid tube. The alternating discrete compartments of the first and second fluids flow through the flow chamber and are analyzed by the substance detector.03-15-2012
20110020859DEVICE COMPRISING A COLOURED AND BIODEGRADABLE POLYMER LAYER FOR ANALYZING THE AGE AND/OR QUALITY OF A NATURAL PRODUCT (INTEGRATED FRESHNESS INDICATOR) - The present invention relates to the field of analyzing the age and/or quality of certain natural products, for example foods. The invention also relates to devices for analyzing said age and/or quality as well as to methods for preparing such devices, to methods for analyzing natural products and to their use.01-27-2011
20120156716Methods For The Isolation, Accumulation, Characterization And/Or Identification Of Microorganisms Using A Filtration And Sample Transfer Device - The present invention is directed to methods, devises and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. In one embodiment, a filtration and sample transfer device is used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation microorganisms, the isolated and/or accumulated microorganisms can be analyzing to acquire measurements for the characterization and/or identification of said microorganisms.06-21-2012
20110318776Method for identifying germs in a liquid medium - This invention relates to a method for identifying germs in a liquid medium, comprising adding a membrane detergent to the liquid medium containing components of a host infected with germs, i.e. cellular components and proteins from the extracellular environment, so as to release the germs from these components without degrading them, and in that the germs that have grown in the liquid medium are analysed by MALDI-TOF MS.12-29-2011
20120003685DIAGNOSTIC COMPOSITION AND ARTICLE FOR MONITORING INTRAVAGINAL INFECTIONS - The present invention provides a non-toxic diagnostic composition for intravaginal monitoring of vaginal infections. The present invention further provides a diagnostic article for intravaginal monitoring of vaginal infections with the article including an absorbent material for absorbing vaginal secretions and a composition suitable for identification of the pH or the buffer capacities associated with vaginal secretions. The diagnostic composition provides a visible indication of vaginal infections, such as bacterial vaginosis.01-05-2012
20120003686CULTURE PLATE HOLDER - The present disclosure describes holder devices, testing kits that include the holder devices, and methods for using the holder devices to allow a user to dispense an aqueous test sample from a sampling device onto a culture plate device having a cover sheet, whereby the cover sheet is supported in an open position by the holder while the user dispenses the aqueous test sample onto a base member of the culture plate device.01-05-2012
20090104652METHOD AND APPARATUS FOR VIABLE AND NONVIABLE PROKARYOTIC AND EUKARYOTIC CELL QUANTITATION - A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.04-23-2009
20110086384IDENTIFICATION OF BACTERIAL SPECIES AND SUBSPECIES USING LIPIDS - The use of free, extractable lipids found in bacteria for identification of bacterial species and subspecies is described. Bacteria have been found to differ sufficiently in their extracted lipid compositions to effect identification using thin layer chromatographic techniques. 04-14-2011
20120115182MASS SPECTROMETRIC DIAGNOSIS OF SEPTICEMIA - The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from blood-stream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour.05-10-2012
20120115183Detector Arrangement for Blood Culture Bottles with Colorimetric Sensors - A detector arrangement is disclosed for a blood culture bottle incorporating a colorimetric sensor which is subject to change of color due to change in pH or CO05-10-2012
20090246822Composition for cell permeabilization comprising NOG, HMP, rubidium chloride and/or lithium chloride for detecting living cells on a membrane - The present invention relates to a composition for the permeabilization of the walls of microorganisms comprising the combination of octyl-β-D-glucopyranoside (NOG), sodium polyphosphates (HMP), and a salt chosen from lithium chloride or rubidium chloride, and a method for the enumeration and identification of cells on a membrane using said composition.10-01-2009
20090023180Reduction of Shading in Microalgae - Methods provided herein are directed to increasing the efficiency of light utilization of photosynthetic microorganisms. Also provided are screening assays, genetic constructs, and photosynthetic microorganisms for increasing light utilization efficiency and production of molecules such as ATP, oxygen, hydrogen, and recombinant proteins. Methods provided herein can be performed with any photosynthetic microorganism, including prokaryotic and eukaryotic microorganisms.01-22-2009
20090117609USE OF TETRACYSTEINE TAGS IN FLUORESCENCE-ACTIVATED CELL SORTING ANALYSIS OF PROKARYOTIC CELLS PRODUCING PEPTIDES OR PROTEINS - A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.05-07-2009
20090017490DNA Fragment Method for Producing Transformant for Protein Production and Utilization Thereof - An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.01-15-2009
20110070605REVERSIBLE MHC MULTIMER STAINING FOR FUNCTIONAL PURIFICATION OF ANTIGEN-SPECIFIC T CELLS - The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.03-24-2011
20110091932Automated extraction and purification of samples using optical tweezers - An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed; and an output through which unselected particles of the sample are removed. At least one buffer input channel is provided. The input channel may allow sedimentation of the sample into the chamber by gravity. In another arrangement, the system includes an optical trapping system; a first channel through which the sample is introduced; a second channel through which buffer is introduced; wherein the optical tweezers are used to move selected particles of the sample from the first channel to the second channel. The selected particles may be sperm. The optical tweezers preferably utilize holographic optical trapping.04-21-2011
20110091931Methods and Kits for Determining Oxygen Free Radical (OFR) Levels in Animal and Human Tissues as a Prognostic Marker for Cancer and Other Pathophysiologies - Described herein are methods and techniques for the determination of reactive oxygen species in resected animal and human tissues. Also described herein are methods for determining the efficacy of anti-oxidant, oxidative stress modulatory, or anti-inflammatory drug chemoprevention or chemotherapy for the prevention and/or therapy of chronically inflamed and/or progressive cancers and pathophysiologies.04-21-2011
20110008824DEVICE AND METHOD OF BACTERIA DETECTION - A bacteria measurement device and method are provided, which separate bacteria from a target object and attach the bacteria to an antibody with fluorescein isothiocyanate applied thereto, generate light using a light emitting unit, and excite the fluorescein isothiocyanate, and in which the excited fluorescein isothiocyanate secondarily emits light, and a light receiving unit receives a light output signal generated through the secondary emission, converts the light output signal into an electrical signal, and converts an analog signal which is the electrical signal into a digital signal, thus measuring the amount and type of bacteria.01-13-2011
20100028938METHOD OF SCREEING FOR PATHOGENS - Disclosed is a method of screening for the presence of MRSA in a subject, the method comprising the steps of: 02-04-2010
20120252057METHOD AND DEVICE FOR DETECTING TUMOROUS LIVING CELL TISSUE - The invention relates to a method and to an apparatus for recognizing tumorous living cell tissue. It furthermore relates to a method and to an apparatus for recognizing tumorous cell tissue at collected living cell tissue samples. In the method, electromagnetic radiation is emitted with local definition onto cell tissue by a radiation source and, after deactivation of the radiation source, the decay behavior of the inherent fluorescence intensity of the cell tissue excited by the electromagnetic radiation is detected at the cell tissue in a time resolved and spectrally resolved manner at known sampling rate(s) for at least one wavelength using a detector. The difference autocorrelation function C(t) of the intensity decay behavior is determined using the determined measured intensity values, the fractal dimension D10-04-2012
20110104743Dynamic Light Scattering for in vitro Testing of Bodily Fluids - A method of diagnosing a pathological condition by detecting microparticles in a sample of bodily fluid using dynamic light scattering (DLS) is disclosed. The detection of microparticles in the bodily fluid by DLS may be used as an indicator of existing disease, to evaluate a risk of disease, as well as to monitor the efficacy of a treatment for disease.05-05-2011
20120164681Method for Detection or Identification of Bacteria or Bacterial Spores - A method and apparatus for detecting bacteria and bacterial spores are disclosed in which a sample is assessed for the presence of bacteria and/or bacterial spores by reference to horizontally polarized fluorescent light.06-28-2012
20120122149Absorbent bead concentration method that eliminates the need for centrifugation - This invention is a technique that enables the concentration of cells, microorganisms, parasite eggs and cysts using absorbent beads, eliminating the need for centrifugation.05-17-2012
20120315665METHOD FOR DETERMINING A MARKER IN SMALL VOLUME OF A SAMPLE OF A BODILY FLUID - The invention refers to a method for determining a marker in a small volume of a sample of a bodily fluid, the method comprising the steps of: providing a flow test element having a plurality of functional zones (12-13-2012
20100248297PARTICLES AND MANUFACTURING METHODS THEREOF - Particles and manufacturing methods thereof are provided. The manufacturing method of the particle includes providing a precursor solution containing a precursor dissolved in a solution, and irradiating the precursor solution with a high energy and high flux radiation beam to convert the precursor to nano-particles. Particles with desired dispersion, shape, and size are manufactured without adding a stabilizer or surfactant to the precursor solution.09-30-2010
20100248298MASS SPECTROMETRIC IDENTIFICATION OF MICROORGANISMS IN COMPLEX SAMPLES - Microorganisms are identified as present in a complex sample or mixed culture by acquiring a mass spectrum of the sample and comparing it to combination spectra, each of which is formed by combining at least two reference mass spectra of known microorganisms. Microorganisms corresponding to the reference spectra used to form the combination spectrum are identified as present in the sample if that combination spectrum exhibits a better match with the sample mass spectrum than any one of reference mass spectra used to form that combination spectrum. It is also possible to identify microorganisms by forming a difference spectrum by subtracting a reference mass spectrum from the sample mass spectrum and comparing the difference spectrum to the reference mass spectra.09-30-2010
20110183370OPTICAL IMAGING FOR IDENTIFYING CELLS LABELED WITH FLUORESCENT NANOPARTICLES - An optical detection system for identifying target bacterial cells in a food sample, comprising a first fluorescent nanoparticle dye labeling specifically the target cells. A second fluorescent dye reacts non-specifically with all bacterial cells. An imaging unit optically excites the fluorescent dyes and captures images of the sample for each of two different wavelength bands emitted as a result of the excitation of the dyes. A processing unit identifies the target cells from the sample and comprises an image processing unit for processing the images of the sample. A target cell identifier identifies the target cells in the images by detecting individual units present at a same location in both of the processed images. An output unit provides data related to the identified target cells in the sample. A method is also provided.07-28-2011
20120164683BUFFY COAT SEPARATOR FLOAT SYSTEM AND METHOD - A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.06-28-2012
20120258487MICROFLUIDIC PLATFORM FOR SYNTHETIC BIOLOGY APPLICATIONS - This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of said biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to for a sequenced order of droplets carrying different components of said biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of said biological construct to permit said components to react with each other in one or more reactions contributing to the assembly of said biological construct.10-11-2012
20120135457APPARATUS AND METHOD FOR THE DETECTION AND ANALYSIS OF PARTICLES IN FLUIDS - The invention relates to apparatus for the detection of particles and for particle analysis. The apparatus comprises a sample holder comprising a base and a projection extending from the base. The base includes a contact region where, in use, the surface of a fluid sample may contact the projection. The surface of at least the contact region of the projection exhibits properties that allow the surface of the contact region to be substantially wetted by a fluid sample when the apparatus is in use so that the fluid sample forms a meniscus having its apex in contact with the contact region of the projection. The invention also relates to methods for using the apparatus.05-31-2012
20120135456METHOD AND KIT FOR DETECTION OF GUAIACOL-PRODUCING BACTERIUM - Disclosed is a method for detecting guaiacol-producing bacteria in a specimen comprising culturing the specimen or a dilution thereof on a plate of a solid medium for acidophilic bacteria comprising a compound represented by the following formula, wherein R is —H, —OH, —C(O)H, —C(O)CH05-31-2012
20120135455Smart Packaging For Detecting Microorganisms - The present invention relates to novel smart packaging, designed using a novel material comprising a partially polar adsorbent solid base impregnated with a solution of vanillin, which allows the growth of microorganisms in different types of products to be detected visually without having to be in direct contact with the microorganism or with the medium containing same.05-31-2012
20120135454METHODS FOR SEPARATION, CHARACTERIZATION AND/OR IDENTIFICATION OF MICROORGANISMS USING SPECTROSCOPY - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements.05-31-2012
20120077222Method and Device for Determining the Microbiological Contamination in an Environment - The method comprises providing at least one sampling plate and at least one supporting member in said environment with the supporting member being suitable for detachably receiving the sampling plate; and removing the plate from the supporting member after a period of time such that the microbiological contamination in said environment can be determined by analyzing said sampling plate. A device is provided for carrying out said method comprising the supporting member carrying one or more sampling plates, attaching means for detachably fixing the sampling plates therein and releasing means for removing of the sampling plate from the supporting member.03-29-2012
20120077221DETECTION DEVICE AND METHOD FOR DETECTING ANALYTE - There is provided a detection device that comprises a light source, a light sensor and an at least partially light-transmitting element that supports growth of at least one analyte selected from among fungi and bacteria. The light source, the at least partially light-transmitting element and the light sensor are positioned relative to one another and oriented relative to one another such that when the light source is illuminated, at least some of the light emitted from the light source passes through the at least partially light-transmitting element and is received by the light sensor. There is also provided a method of detecting an analyte, comprising illuminating a light source to emit light, and detecting an intensity of light that passes through an at least partially light-transmitting device that comprises an at least partially light-transmitting element that supports growth of at least one analyte selected from among fungi and bacteria.03-29-2012
20120252059FLUORESCENT DERIVATIVES OF POLYAMINES, METHOD FOR PREPARING SAME AND APPLICATIONS THEREOF AS DIAGNOSIS TOOLS IN THE TREATMENT OF CANCEROUS TUMORS - The invention relates to novel fluorescent derivatives of polyamines having a benzoxadiazole group, to a method for preparing the same and to the use thereof as diagnosis tools for emphasising the polyamine transport system in cancerous cells in order to adapt the treatment thereof, and thus for selecting patients carrying such tumours in order to adapt their treatment. The derivatives are of the formula (I) or a pharmaceutically acceptable salt thereof, in which: R10-04-2012
20100273208MICROORGANISM TESTING APPARATUS - To eliminate the necessity of a dedicated optical system and the flowing of fluorescent microparticles for aligning excitation light with a flat plate-shaped flow cell which internally includes a flow path, a microorganism testing apparatus includes: a first detector that detects fluorescence emitted from microorganisms flowing through a detection flow path when a microorganism detection unit included in a microorganism testing chip is irradiated with excitation light, and converts the fluorescence to an electrical signal; and a second detector that detects scattered light similarly emitted from the microorganisms flowing through the detection flow path, and converts the scattered light to an electrical signal. The alignment of the detection flow path is performed in the direction of the optical axis of the excitation light by controlling and moving a stage having the microorganism testing chip mounted thereon based on the intensity of fluorescence detected by the first detector.10-28-2010
20100273207Methods for Diagnosis of Sepsis and Risk of Death - The present invention is directed to methods for diagnosis of sepsis and high risk of sepsis death. Such methods are directed to subjects presenting to healthcare facilities with symptoms for sepsis. Clinico-metabolomic classifiers are tested and compared in the methods of the present invention.10-28-2010
20090203065CELL CULTURE ARTICLE AND SCREENING - A method for producing a cell culture article having a synthetic polymer layer for incubating with cells includes diluting one or more (meth)acrylate monomers in a solvent and dispersing the diluted monomers on a surface of the cell culture article. Some or substantially all of the solvent is removed and the monomers are then polymerized on the surface of the article to form the synthetic polymer layer attached to the surface of the article.08-13-2009
20120315664ASSEMBLY AND METHOD FOR THE FILTRATION OF A LIQUID AND USE IN MICROSCOPY - An assembly and method are disclosed for the filtration of a liquid and the use thereof, wherein a supporting body is designed in a recess of a carrier and a filter membrane lies flat on the supporting body. The filter membrane and the supporting body are designed to be permeable to liquids and thus serve as filters, in particular for filtering tumor cells from blood. The carrier can having standard shapes of an object carrier for microscopy and the filtration residue on the filter membrane can be easily handled and examined in the microscope. As a result of the filter membrane lying level on the supporting body, the filtration residue can be particularly well examined microscopically.12-13-2012
20100291619Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample - A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.11-18-2010
20100291618Methods for rapid identification and/or characterization of a microbial agent in a sample - A method of testing a specimen sample contained within a sealed specimen container. A test sample is removed from the specimen container with a disposable sampling device. Non-microbial agent components of the test sample are lysed, thereby producing a lysed sample. The lysed sample is delivered to a disposable separation device. A microbial agent present in test sample is concentrated within the disposable separation device. The concentrated microbial agent is interrogated, e.g., spectroscopically.11-18-2010
20100291617RAPID DETECTION OF VOLATILE ORGANIC COMPOUNDS FOR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS IN A SAMPLE - In various embodiments, the invention relates to a method for identifying the presence of particular bacteria in a sample. The method includes collecting a sample that includes or has been exposed to the particular bacteria and detecting, in the sample, at least one volatile organic compound indicative of the presence of the bacteria.11-18-2010
20120225449Method and appraratus utilizing enzyme substrates producing slow diffusing fluorescent product appearances and chromogens, and use in combination with fast diffusing product appearances - A new method of rapid detection of cells, microorganisms, or other items is described using various combinations of indicator enzyme substrates which can yield fluorophoric and chromophoric appearances due to reaction with enzymes. An aspect of the invention is the use of a family of compounds producing both slow diffusing fluorophoric appearances and a family of compounds identified as dual enzyme substrates producing both fluorophoric and chromogenic appearances.09-06-2012
20120258488Systems and Methods for Electrophysiological Activated Cell Sorting and Cytometry - Provided herein are methods and systems for non-genetic, label-free cell purification (i.e., cell cytometry and sorting), which classifies cells based on their spontaneous electrophysiological response or their electrophysiological response to a stimulus. For example, in one embodiment, there is provided a method of cell sorting comprising: stimulating a cell with a stimulus; sensing a response evoked by the cell based on the stimulus; identifying a phenotype of the cell based on the evoked response; and sorting the cell based on its phenotype. In one embodiment, the stimulus may be an electrical stimulus, a mechanical stimulus, an optical stimulus, a thermal stimulus, a chemical stimulus, or any combination thereof. The cell phenotype may be, for example, cardiomyocytes, neurons, smooth muscle cells, or pancreatic beta cells.10-11-2012
20120264164LYMPH NODE SPECIMEN COLLECTION KIT AND METHOD OF PATHOLOGICAL ANALYSIS FOR LUNG CANCER DIAGNOSIS USING SUCH A KIT - A unique system for the correlation between removed lymph nodes for lung cancer diagnosis and pathological analysis thereof is provided. Such a system includes the removal of certain lymph nodes from a suspected or known lung cancer patient with subsequent categorization thereof and placement within a properly divided and labeled specimen collection kit. Through the utilization of such a separation and placement allows and facilitates understanding and non-verbal communication between a surgeon and a pathologist in order to denote the location of the removed lymph nodes in relation to a known or suspected lung cancer tumor or growth. The overall diagnostic method, including the important communicative properties accorded both the particular surgeon and pathologist, is encompassed within this invention, as well as the specific collection specimen kit that permits the surgeon proper distinction of specific removal lymph nodes in relation to their location within the patient's body.10-18-2012
20110124039STAINING PROCESS AND APPARATUS USED IN BACTERIOLOGY - The present invention refers to a staining process used in bacteriology able to standardize and make Gram's staining procedure easier and faster, preventing variations in its process.05-26-2011
20110124038AUTOMATED TRANSFER MECHANISM FOR MICROBIAL DETECTION APPARATUS - The present invention is directed to a method and automated transfer means for transferring a container within an apparatus. The apparatus of the present invention may include a means for automated loading, a means for automated transfer and/or a means for automated unloading of a container (e.g., a specimen container). In one embodiment, the apparatus can be an automated detection apparatus for rapid non-invasive detection of a microbial agent in a test sample. The detection system also including a heated enclosure, a holding means or rack, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system.05-26-2011
20130171687DEVELOPMENT OF A DETECTION MICROSYSTEM - This invention relates to a device for detecting fungal contamination in an interior environment, including: 07-04-2013
20130171688STERILISATION INDICATOR - A sterilization indicator having a compound that is of the heating type when put into contact with atoms of oxygen O and/or nitrogen N; and a thermochromic dye, in thermal contact with the compound.07-04-2013
20130171689CAPTURE OF MICRO-ORGANISMS - Micro-organisms, including fungi, viruses and bacteria such as 07-04-2013
20080299604METHODS AND TOOLS FOR DETECTING COLLAGEN DEGRADATION - Fluorophore-labeled collagen covalently bound to a cell culture vessel can be used to assay collagen degradation by a variety of cell types, including osteoclasts and tumor cells. Such assays provide a high throughput platform for rapid screening of large numbers of potential modulators of, for example, tumor metastasis or osteoclast differentiation and/or function.12-04-2008
20120264163APPARATUS AND METHOD FOR DETECTING BACTERIAL GROWTH BENEATH A WOUND DRESSING - An apparatus and method are provided for visually monitoring, detecting, and/or determining the presence, absence, and/or growth of harmful or potentially harmful bacterial microorganisms beneath a wound dressing, in one example used to cover an indwelling central venous catheter or other catheter. A bacteria detection apparatus includes a barrier membrane, a permeable membrane for placement proximate a wound or a catheter insertion site, and an indicator between the barrier membrane and the permeable membrane for indicating the presence of bacteria proximate the permeable membrane. A method of using a bacterial growth detection apparatus is also provided.10-18-2012
20110003328SYSTEM FOR DETECTING MICROBIAL CONTAMINATION - The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hypobaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to said hypobaric chamber, and (iii) a vacuum pump adapted for being fluidly connected to said hypobaric chamber, said system further comprising a kit for detection of micro-organisms adsorbed to said adsorbent material, wherein said kit is based on enzymatic detection using chromogenic and/or fluorescent substrate analogues.01-06-2011
20110003330MICROFLUIDIC DEVICE - The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard data storage capabilities. In certain other embodiments, the microfluidic devices have onboard anticoagulants. In certain other embodiments, the microfluidic devices have onboard test and control channels. In certain other embodiments, the microfluidic devices have integrated collection media. In certain other embodiments, the microfluidic devices have multiple onboard test channels. In certain other embodiments, the microfluidic devices have localized temperature control. In certain other embodiments, the microfluidic devices have anatomy simulating regions. In certain other embodiments, the microfluidic devices have complete assay capabilities. In certain other embodiments, the microfluidic devices have dissociable sections. In certain other embodiments, the microfluidic devices have means for performing functional assays.01-06-2011
20120237967Method for Microbiological Testing - Costly enrichment medium used for microbiological testing of nuts and other moisture-absorbing food products is conserved by saturating the product with water or other inexpensive liquid prior to contacting the product with enrichment medium.09-20-2012
20110045526SELECTION OF USEFUL FUNGAL STRAINS - The present invention provides methods for screening for a desired filamentous fungus that produces cellulase more efficiently and/or with increased specific activity. It also provides filamentous fungi that produce cellulase more efficiently.02-24-2011
20110045525Method for Flagging a Sample - A hematology analyzer is provided. In certain embodiments, the hematology analyzer comprises: a) a flow cell; b) a light source for directing light to the flow cell; c) a plurality of detectors for detecting a plurality of optical characteristics of a blood cell passing through the flow cell; and d) a data analysis workstation programmed to: i. enumerate test blood cells passing through the flow cell; and ii. flag a blood sample as containing lysis-resistant red blood cells or fragile white blood cells.02-24-2011
20090081721HIGH-THROUGHPUT CELL ASSAYS - This invention provides high-through put methods and systems to identify and/or classify cells present in a sample. In one aspect, the method identifies the cell by determining the amount of thermal energy required to disrupt cell membranes. In another aspect, a method is for determining if an agent such as a drug will inhibit the growth of a cell by monitoring the amount of energy required to maintain a substantially constant temperature in a sample containing the cell grown in the presence of an agent or drug is provided.03-26-2009
20110236924SYSTEM AND METHOD FOR PROCESSING SAMPLES - A system and method for processing samples. The system can include a loading chamber, a detection chamber positioned in fluid communication with the loading chamber, and a fluid path defined at least partially by the loading chamber and the detection chamber. The system can further include a filter positioned such that at least one of its inlet and its outlet is positioned in the fluid path. The method can include positioning a sample in the loading chamber, filtering the sample in the fluid path to form a concentrated sample and a filtrate, removing the filtrate from the fluid path at a location upstream of the detection chamber, moving at least a portion of the concentrated sample in the fluid path to the detection chamber, and analyzing at least a portion of the concentrated sample in the detection chamber for an analyte of interest.09-29-2011
20110236923METHOD FOR STAINING AND SORTING OF A SMALL VOLUME OF SPERM - The present invention relates to a method of sorting of a small volume or low concentration stained sperm sample, the method includes: (a) diluting the stained sperm cells in a ratio of 1:1 or less by volume with a dilution buffer to obtain a diluted volume of said stained sperm sample; (b) placing the diluted volume of the stained sperm sample on a flow cytometer to initiate the sorting process; (c) sorting the stained sperm cells in the diluted volume based upon a preselected sperm cell or stain characteristic; and (d) collecting sorted sperm cells.09-29-2011
20100233756Animal Feed Kibble with Protein-Based Core and Related Methods - Kibble-type animal feeds and pet foods including a vegetable protein-based core matrix and a coating of a fat and at least one additive are described. The coating may include a probiotic enriched coating. Methods of forming the kibble-type animal feeds and pet foods are also described. Probiotic coating for a kibble showing acceptable stability and bioactivity are disclosed and methods for assessing the bioactivity of a probiotic in a food composition are also described.09-16-2010
20120276579ORGANIC COMPOUNDS - The present invention relates to a novel selection system for use in a eukaryotic cell culture process and for expression of a recombinant product of interest. The selection system is based on the introduction of an exogenous functional membrane-bound folate receptor gene together with the polynucleotide or gene encoding the product of interest into a eukaryotic cell and can be widely utilized with eukaryotic cells for which cellular viability is dependent upon folic acid uptake.11-01-2012
20120276581METHOD FOR PRESERVING CANCELLOUS BONE SAMPLES AND PRESERVED CANCELLOUS BONE TISSUE - The claimed subject matter is based on the finding that it is possible to cryopreserve cancellous bone tissue with viable cells (after reconstitution by either thawing or rehydration), the viability after reconstitution being comparable to that of a fresh sample (without freezing) and of beneficial value for use in, for example, transplantation. Thus, provided is a method for cryopreserving a cancellous bone sample. The cryopreserved cancellous bone sample can be in dry form, for example, lyophilized. Also provided is a cryopreserved, in dry form, bone sample and the use of a cryopreserved bone sample; a method for identifying such cryopreserved cancellous bone tissue and uses thereof.11-01-2012
20120276580MICROORGANISM CONCENTRATION PROCESS AND CONCENTRATION AGENT FOR USE THEREIN - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing an adsorption buffer-modified inorganic concentration agent that is prepared by a process comprising (1) contacting at least one inorganic concentration agent with at least one cation-containing salt solution, so as to wet at least a portion of the inorganic concentration agent and (2) drying the resulting at least partially wet inorganic concentration agent; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the adsorption buffer-modified inorganic concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the adsorption buffer-modified inorganic concentration agent.11-01-2012
20120276578Phasor Method to Fluorescence Lifetime Microscopy to Discriminate Metabolic State of Cells in Living Tissue - “A label-free imaging method to monitor stem cell metabolism discriminates different states of stem cell as they differentiate in a living tissues. We use intrinsic fluorescence biomarkers and the phasor approach to Fluorescence Lifetime Imaging Microscopy (FLIM). We identify and map intrinsic fluorophores such as collagen, retinol, retinoic acid, flavins, nicotinamide adenine dinucleotide (NADH) and porphyrin. We measure the phasor values of germ cells in 11-01-2012
20120288888MICROBIAL DETECTION ARTICLE - The disclosure provides an article for the detection of a microorganism in a liquid sample. The article comprises a microporous membrane and a barrier layer to selectively regulate the contact between the sample and a detection reagent. A method of use is also provided.11-15-2012
20130011872STABLE ISOTOPIC BIOMARKER MEASUREMENT FOR THE DETECTION OF CANCER AND THE DETERMINATION OF EFFICACY OF TREATMENT IN DIAGNOSED CANCER PATIENTS - The present invention relates to a method of detecting the presence of cancer cells in an animal such as a human or other mammal, comprising administering a measured volume of air to the subject wherein 01-10-2013
20100129858Method for separation, characterization and/or identification of microorganisms using spectroscopy - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.05-27-2010
20130017570METHOD FOR MONITORING STATE OF DIFFERENTIATION IN STEM CELL - A method for monitoring the differentiation state of a stem cell, includes a step (A-1) of culturing a stem cell into which a fusion gene of a promoter region of a differentiation state detection marker gene and a luminescent protein-coding gene is introduced, a step (A-2) of culturing the stem cell under a differentiation-inducing condition after the step (A-1), and a step (A-3) of capturing images of luminescence emitted by expression of the luminescent protein-coding gene in the stem cell, over at least a given period of the steps (A-1) to (A-2).01-17-2013
20110159536DEVICE FOR CAPTURING OBJECT AND METHOD FOR USING THE SAME - An object of the present invention is to provide an object-capturing device which accurately detects objects such as microorganisms captured at a test site. The object-capturing device of the present invention includes a capturing dish holding a carrier, which captures objects (microorganisms), on a first side of the capturing dish. The capturing dish has a through hole extending from the first side to a second side of the capturing dish. The object-capturing device is used in a way such that, after the objects are captured with the carrier directed upward, the carrier is directed downward and reagents for detecting the objects are contacted with the objects captured on the carrier through the through hole.06-30-2011
20110159535PREPARATION AND USE OF NUCLEATED RED BLOOD CELL SIMULATING PARTICLES AND HEMATOLOGY CONTROL MIXTURES - The present disclosure provides a nucleated red blood cell simulating particle, which may be leukocytes bound to a fluorescent-staining inhibitor capable of stably binding to the nucleus or a nucleic acid in a cell so as to reduce the binding capacity of the particles to a fluorescent dye during their detection. The present disclosure also provides a method for preparing nucleated red blood cell simulating particles, including the following steps: (a) obtaining purified leukocytes; (b) suspending the leukocytes in a cell treatment solution containing a fluorescent-staining inhibitor which stably binds to the nucleus or a nucleic acid in a cell, and (c) washing the obtained product. The present disclosure also provides a hematology control mixture containing the nucleated red blood cell simulating particles. In addition, the present disclosure describes the use of the nucleated red blood cell simulating particles and the hematology control mixtures comprising the same, for the quality control of a blood cell analyzer.06-30-2011
20110159534METHOD AND CULTURE MEDIUM FOR ENHANCED DETECTION OF MYCOBACTERIUM - The present invention relates to an improved culture medium and method for the enhanced growth and detection of 06-30-2011
20130171685METHOD AND APPARATUS FOR CHARACTERIZING BIOLOGICAL OBJECTS - In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object in a contactless fashion. A measurement and a further measurement are performed on the biological object in order to ascertain a response of the biological object to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object and/or capturing data using digital holographic microinterferometry (DHMI). The biological object is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field.07-04-2013
20130171686METHOD OF TREATING MINE-INFLUENCED WATER AND SOIL - The present invention is directed to a method of trans-seasonal bioremedial treating of mine-influenced water and soil (MIWAS) in situ with microorganisms for removal of contaminants containing heavy metals compounds. The method comprises the steps of: a) sampling indigenous microorganisms from the MIWAS site being treated; b) identifying and/or isolating indigenous microorganisms that consume selected compounds to be treated in the MIWAS; c) propagating at least one of the indigenous microorganisms identified or isolated in step b); and d) reintroducing propagated microorganisms either on the soil surface or subsurface at the MIWAS site together with a source of nutrients and a carbon source for the microorganisms; wherein when the microorganisms, nutrients, and carbon source are reintroduced on the soil surface, they are subsequently buried.07-04-2013
20080248518E.coli and salmonella test kit - A method for testing for the presence of bacteria in the field includes using a mixer and frother or a reagent to disrupt bacteria cells. A sample is collected from a source suspected to be contaminated by bacteria. A mixer and frother or similar mixer is used to mix the sample, or a reagent is added to the sample, to form an elute by disrupting bacteria cells present in the sample to expose bacteria enzymes. A reagent pad is immersed in the elute and then withdrawn from the elute, or a drop of the elute is deposited on a reagent pad. After a waiting period, the reagent pad is observed to determine if the bacteria is present in or on the source. The source may be a body of water, produce, prepared food, a food preparation area, or food processing equipment. The reagent is generally a lysis reagent.10-09-2008
20080227142Invasive chemometry - The invention relates to methods and devices for assessing one or more components of a selected tissue in an animal. The present invention permits non-invasive assessment of tissue components in a body structure containing multiple tissue types by assessing multiple regions of the animal's body for an optical characteristic of the tissue of interest and separately assessing one or more optical (e.g., Raman or NIR) characteristics of the tissue component for one or more regions that exhibit the optical characteristic of the tissue of interest.09-18-2008
20080220467Filter Snapper - A device for applying an axial compressive force to a fluid contamination detection system comprising a cup joined by a frangible connection to a filter ring, the force being sufficient to break the frangible connection and collapse a portion of the ring into the cup without damaging the ring. The device includes a stop element and a platform and an actuating mechanism for reciprocally moving the platform relative to the stop element. In a first position, the distance between the platform and the stop element is greater than the axial length of the detection system when the cup and the filter ring of the funnel are connected to each other, and in a second position, the distance between the platform and the stop element is less than the axial length of the detection system when the cup and the filter ring of the funnel are connected to each other.09-11-2008
20130177938COLLECTION BROTHS FOR MICROORGANISMS - A collection broth used for recovering microorganisms on a surface is provided. The collection broth includes a buffering system and a neutralization system. All components of the collection broth are both food-grade and hypoallergenic.07-11-2013
20130177936METHOD OF CANCER DIAGNOSIS USING THE ANALYSIS OF ISOPOPES - Disclosed is a method of diagnosing cancer on the basis of the quantitative analysis of blood or tissue isotopes. The method can accurately diagnose cancer even when it is too small for current conventional technology to diagnose.07-11-2013
20130177937CONTAINER, A CLOSURE FOR A CONTAINER, AND A BASE FOR A CONTAINER - A container, comprising: a body; and, a closure; wherein the closure has a lid hinged to a ring mounted on the body, the lid moveable from an open position to a closed position; wherein the lid has a tab hinged thereto for: inserting into and engaging a slot formed in the ring as the lid is moved to the closed position from the open position to thereby lock the lid in the closed position; or, receiving and engaging a pin head of a pin mounted on the ring in a hole formed in the tab to thereby hold the lid in the closed position, the pin configured to detach from the ring when the tab and lid are returned to the opened position.07-11-2013
20130177939OPTICAL SENSOR AND METHOD FOR INDICATING THE AGE OR QUALITY OF A NATURAL PRODUCT - The present invention relates to the field of analyzing the age and/or quality of certain natural products, for example foods. The invention also relates to devices for analyzing said age and/or quality as well as to methods for preparing such devices, to methods for analyzing natural products and to their use.07-11-2013
20120252061NOVEL YEAST STRAINS FOR THE PRODUCTION OF ALCOHOL - The present invention relates to novel yeast strains intended for alcohol production.10-04-2012
20120252060Self-Renewing Single Human Hematopoietic Stem Cells, an Early Lymphoid Progenitor and Methods of Enriching the Same - This invention relates to human hematopoietic stem cells. Specifically the invention relations to the identification of single human hematopoietic stem cells capable of long-term multilineage engraftment and self-renewal. The invention also relates to an early lymphoid progenitor with monocytic potential, including dendritic cell potential.10-04-2012
20120252058System and Method for the Assessment of Biological Particles in Exhaled Air - A system and method for the deposition and analysis of a sample comprising exhaled particles. The sample may be deposited on a substrate and regions of interest comprising exhaled particles may be targeted using autofluorescence. Regions of interest comprising exhaled particles may then be interrogated to thereby generate a spectroscopic data set. This spectroscopic data set may be compared to a reference data set associated with at least one of the following: a known disease state, a known metabolic state, a known inflammatory state, a known immunologic state, a known infectious state, and combinations thereof.10-04-2012
20110269179COLIFORM DETECTION PROCESS AND KIT FOR USE THEREIN - A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.11-03-2011
20130095520METHOD FOR DETECTION OF UROTHELIAL CANCER - It is to provide a method for detecting urothelial cancer simply and with high accuracy. It is a method for detecting urothelial cancer comprising administering 5-aminolevulinic acid (ALA), a derivative thereof, or a salt of these to a test subject, collecting urine from the test subject, and detecting the presence of fluorescence or amount of fluorescence in the collected urine.04-18-2013
20130095519BIOLOGICAL TISSUE ANALYSIS BY INVERSE SPECTROSCOPIC OPTICAL COHERENCE TOMOGRAPHY - A method and system to measure and image the full optical scattering properties by inverse spectroscopic optical coherence tomography (ISOCT) is disclosed. Tissue is modeled as a medium with continuous refractive index (RI) fluctuation and such a fluctuation is described by the RI correlation functions. By measuring optical quantities of tissue (including the scattering power of the OCT spectrum, the reflection albedo α defined as the ratio of scattering coefficient μ04-18-2013
20130102025METHODS AND COMPOSITIONS FOR IDENTIFYING A CELL PHENOTYPE - The present invention is directed to a method of identifying cancerous or precancerous cells suspended in a liquid carrier, such as, a biological fluid.04-25-2013
20130102026Culture medium and a method for detection of parasites - This invention relates to a culture medium, a kit containing the culture medium and to a method for detection of a parasite such as 04-25-2013
20130102024OPTICAL FLUORESCENCE DUAL SENSORS AND METHODS OF PREPARING AND USING THEM - The present invention relates to an optical fluorescence dual sensor comprising a probe for sensing pH, a probe for sensing oxygen, an intra-reference probe and a matrix. The present invention also relates to methods of preparing an optical fluorescence dual sensor and methods of using them.04-25-2013
20130130308PROCESS FOR DIRECTLY MEASURING MULTIPLE BIODEGRADABILITIES - A method for measuring the biodegradability of organic substrates by the fluorescent and/or colorimetric detection of the microbial activity generated by the addition of organic substrates to a mixture of microorganisms.05-23-2013
20130130306ZN (II) BASED COLORIMETRIC SENSOR AND PROCESS FOR THE PREPARATION THEREOF - A colorimetric chemosensor molecule having a aza-macrocycle Zn (II)-complex (L.Zn) (Scheme 1, Formula 1A) which can recognize selectively and efficiently ATP (Adenosine triphosphate), a biologically significant triphosphate in aqueous medium at pH 7.4 is described. Since ATP is the source of energy in living organisms, L.Zn (Scheme 1, Formula 1A) can also be used as a staining agent in the living cells through binding to ATP, generated in situ during the metabolic process.05-23-2013
20130130307CELL OBSERVATION DEVICE AND CELL OBSERVATION METHOD - A cell observation device is provided with a reflection interference shutter 05-23-2013
20130143258DIAGNOSIS OF CANCER - A method is provided comprising, obtaining an infrared (IR) spectrum of a Peripheral Blood Mononuclear Cells (PBMC) sample by analyzing the sample by infrared spectroscopy; and based on the infrared spectrum, generating an output indicative of the presence of a solid tumor or a pre-malignant condition. Other embodiments are also provided.06-06-2013
20130143259Device For Microbiological Analysis - The device for the microbiological analysis of a support adapted to contain microorganisms marked by a fluorophore agent, said agent being adapted to absorb light energy with an absorption spectrum (06-06-2013
20130143260ALGA IN WHICH PRODUCTION OF PHOTOSYNTHETIC PRODUCTS IS IMPROVED, AND USE FOR SAID ALGA - Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.06-06-2013
20110217729TARGETED CELL SEPARATION AND ENUMERATION DEVICE AND THE USE THEREOF - This disclosure describes a portable device that conveniently applies cancer patient blood sample into a test strip to determine the level of the patient's metastatic circulating tumor cells. Proper use of this device provides end users with the diagnostic and prognostic information of the cancer patient, and any effect of applied therapies. The device can also be used to screen effective drugs for any known metastatic phenotype disruption.09-08-2011
20080199904Apparatus and method for detecting and identifying microorganisms - The present invention is an apparatus for detecting the presence, quantity and identity of one or more microorganisms in a sample and a method for using the same. The apparatus is composed of one or more chambers and a sensing element for sensing microorganisms. In particular embodiments, the sensing element is an array of chemoresponsive dyes deposited on a substrate in a predetermined pattern combination, wherein the combination of the dyes have a distinct and direct spectroscopic, transmission, or reflectance response to distinct analytes produced by the microorganism which is indicative of the presence, quantity and identity of the microorganism.08-21-2008
20130149737SYSTEM AND METHOD FOR SORTING CELLS - Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.06-13-2013
20130149738MICROBIAL DETECTION SYSTEM AND METHODS - The disclosure provides culture devices and methods for a microorganism in a sample. The devices include a base member, a cover sheet, an adhesive layer coupled to the base member or the cover sheet, and a cold water-soluble gelling agent disposed on the base member; wherein the devices are substantially optically transmissive when the gelling agent is hydrated with a clear liquid. Methods of use include detecting or enumerating microorganisms. The methods further provide for detecting a microorganism by detecting the presence or size of an abiogenic gas bubble (06-13-2013
20110275113MASS SPECTROPHOTOMETRIC DETECTION OF MICROBES - A method of detecting specified target microbes in different types of sample uses only one to two cultivation steps for the enrichment of the target microbes from the sample, preferably in selective culture media, combined with a mass spectrometric detection method that identifies the target microbes in mixtures with other microbes even if the target microbes account for only a small proportion of the mixture. The sample may be a food sample, a sample from bodies of water used for bathing, a soil sample, a swabbed sample, a stool sample, an impactor sample with collected aerosol particles, amongst many others. The detection method is several days faster than standard methods and less expensive.11-10-2011
20110275112Microbial growth detector - The disclosure generally relates to a test device that detects microorganism growth by detecting a gas metabolite (e.g., carbon dioxide) produced during the growth of bacteria or other microorganism in a tested sample. The test device can contain a culture growth media separated from a detection area by a gas-permeable membrane. The gas-permeable membrane permits carbon dioxide to permeate into the detection area. The detection area includes a solidified mixture of pH indicators and a gelling agent in the form of a semi-permeable matrix. The optical properties, including the absorbance of light at various wavelengths, of the detection solution change with alterations in carbon dioxide concentration. This test device can then be placed in an incubation and optical detection instrument to monitor changes in optical properties of the detection are induced during microorganism growth in the culture medium.11-10-2011
20100285521ADIPOSE TISSUE COLLECTION AND PRE-PROCESSING DEVICES FOR USE IN LIPOSUCTION PROCEDURE - Devices and methods are described for pre-processing tissue samples containing cells of interest for various therapeutic procedures. Fibrous material may be extracted from the tissue sample through a shredding and separating device, resulting in a non-fibrous sample containing the cells of interest, which may then be washed and centrifuged to remove fluids, oil, and contaminants. The sample may then be digested in a large-volume container, and a piston within the container may push the resulting liberated cell suspension through a cell concentrator such as a hollow-fiber separation module.11-11-2010
20100317052PROCESS FOR DETECTING HELICOBACTER PYLORI USING ALIPHATIC AMIDES - The present invention provides a process for detecting 12-16-2010
20130157305METHOD AND SYSTEM FOR EVALUATION OF GRAFTS - A method and system for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation are provided. In one form, the method includes determining multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation.06-20-2013
20120282649FLUORESCENCE DETECTING APPARATUS, FLUORESCENCE DETECTING METHOD, AND FLUORESCENCE SIGNAL PROCESSING METHOD - Disclosed herein is a fluorescence detecting apparatus. The fluorescence detecting apparatus includes a light-receiving element that receives fluorescence emitted from an object to be measured irradiated with laser light modulated at a predetermined frequency and outputs a fluorescence signal at an adjusted output level; a signal processing unit that mixes the outputted fluorescence signal and a modulation signal with the frequency to generate fluorescence data including information about phase and intensity; and an analyzing device that calculates a first phase shift of the fluorescence emitted from the object to be measured with respect to the modulation signal, calculates a second phase shift by correcting the calculated first phase shift depending on conditions for adjusting the output level, and calculates a fluorescence relaxation time of the fluorescence emitted from the object to be measured using the calculated second phase shift.11-08-2012
20130183711LIQUID CRYSTALS WITH SWITCHABLE WETTABILITY FOR CELL SORTING - Disclosed are methods and apparatuses for identifying and sorting cells based on the cells' response to an external stimulus. Cellular adherence to liquid crystals with tunable wettability is measured before and after an induced change in the liquid crystal wettability. The cell-based liquid crystal reorientation can be measured and used for monitoring and sorting of cells in a label-free manner, and thus provides a positive method for selecting cells, such as stem cells, for use in tissue engineering applications.07-18-2013
20110312021METHOD AND APPARATUS FOR THE NON-INVASIVE MONITORING OF GAS EXCHANGE BY BIOLOGICAL MATERIAL - The invention provides an indirect pressure sensing system for non-invasive measurement of primary pressure in a sealed container, which communicates primary pressure changes from within the container, via a flexible diaphragm, to a secondary chamber wherein there is a defined relationship between the primary and secondary pressures, which enables a pressure sensor in the secondary chamber to generate a signal representing primary pressure in the sealed container, but to remain isolated from the contents of the sealed container. The pressure sensor can provide electrical outputs representing the pressure detected, and the outputs are fed to data processing means capable of producing a measurement of primary pressure. The system can have a liquid culture of cellular material (eg. micro organisms, plant tissue cells, animal cells etc) partially filling the container, whereby the metabolism and/or growth of cellular material causes gas exchanges between liquid and headspace, which can result in primary pressure changes.12-22-2011
20120021456METHOD AND APPARATUS FOR DETECTING THE PRESENCE OF ANISOTROPIC CRYSTALS AND HEMOZOIN PRODUCING PARASITES IN LIQUID BLOOD - An apparatus and method for detecting the presence of anisotropic crystals within a biologic fluid sample is provided. The method includes the steps of: a) disposing the sample within a sample chamber in a sample layer having a height riot greater than about fifteen microns (15μ); b) disposing the sample layer within the sample chamber between a polarizing filter and an analyzing filter; a) disposing the polarizing filter, sample chamber, and analyzing filter in a configuration relative to a light source such that polarized light passes through the sample, and subsequently impinges on the analyzing filter; and d) wherein polar orientations of the polarizing filter and the analyzing filter are such that the polarized light will not pass through the analyzing filter, and light passing through an anisotropic crystal disposed within the sample will pass through the analyzing filter and appear as a point of light.01-26-2012
20120021455Detector Arrangement for Blood Culture Bottles With Colorimetric Sensors - A detector arrangement for a blood culture bottle incorporating a colorimetric sensor which is subject to change of color due to change in pH or CO01-26-2012
20120021454RAPID FRET-BASED DIAGNOSIS OF BACTERIAL PATHOGENS - The invention comprises a substrate for detection of micro-organisms, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeieties, to a di-, or tripeptide consisting of amino acids X1 and X2, or X1, X2 and X3, in which one of them, for example X1, is a D-amino acid and the others, for example X2 and X3, may be any D- or L-amino acid. Said substrate preferably is used for the detection of 01-26-2012
20120021453LABEL-FREE CELL SORTING USING NEAR INFRARED EMISSION - Disclosed are methods and systems for identifying and sorting cells based on a near-infrared emission pattern of the cell in response to excitation at 630±nm. The NIR emission pattern can be used for monitoring and sorting of cells in a label-free manner, and thus provides a positive method for selecting cells, such as stem cells, for use in therapy.01-26-2012
20120028296FECAL SAMPLE COLLECTION AND PROCESSING APPARATUS AND METHOD - A method for collecting and processing biological samples (e.g., fecal samples) is disclosed. The method may include obtaining a system comprising a container, a collector, and a lid. Using the collector, a user may collect a sample. The sample may be inserted within the container. The lid may be secured and the container and sample contained therewithin may be transported to a testing facility. At the testing facility, the container may be used throughout the processing of the sample. Accordingly, the risk of cross-contamination may be reduced.02-02-2012
20120028295SYNERGISTIC COMPOSITION USEFUL AS MICROBIOLOGICAL GROWTH MEDIUM FOR RAPID SCREENING OF PHOSPHATE ACCUMULATING MICROORGANISMS - Screening isolates for phosphate accumulation by quantitative methods requires investment of time, labour and chemicals. There has been paucity of a medium and method for screening phosphate-accumulating microorganisms (PAOs) from the environment. Therefore, a new screening strategy is needed towards isolating effective PAOs in environments with diverse microbial populations and limited organic resources, where it is possible that PAOs are often out-competed by organisms capable of utilising available nutrients more rapidly. In view of these problems, a medium optimal for an efficient screening of P-accumulating microorganisms was developed, which is rapid and allows many PAOs to be screened. Thus, the present invention utilizes Toluidine Blue-O (TBO) a blue coloured dye, which decolorizes due drop in the concentration of phosphate in the medium, as an indicator to quickly evaluate the P-accumulating microorganisms based upon visual observations. Fast decolorization of the dye is an indicator of a superior phosphate accumulating microbe. Therefore, colorimetric reaction using PAM-TBO of the present work was further applied in a novel, extremely high-throughput manner to detect individual microbial isolates, growing on a PAM Petri plate. Adaptation of PAM-TBO assay in screening PAOs in environmental samples for microtitre plate assay enhanced the effectiveness of the assay system efficiently and effectively.02-02-2012
20120028294HOME TEST KIT FOR DETECTING FECAL BLOOD - A home test kit for detecting fecal blood includes a scoop device for collecting a fecal sample, a luminescent for mixing with the sample, a hollow container for containing the sample, water and the luminescent, or luminol during use of the kit. A base may be provided for supporting the container during testing, and the container may include an integrally formed lid. Detection of the blood in the sample is possible because the luminescent undergoes a light-producing reaction that involves, as a reactant or catalyst, blood or blood components or products. The blood is thus visible, or glows in darkened surroundings. The kit is compact, easy to use, and formed from biodegradable material for easy disposal.02-02-2012
20130196366Method and Culture Medium for Enhanced Detection of Mycobacterium - The present invention relates to an improved culture medium and method for the enhanced growth and detection of 08-01-2013
20130196367MODULATORS OF CANDIDA HYPHAL MORPHOGENESIS AND USES THEREOF - The invention relates to modulation of fungal morphology between yeast-to-hyphal growth transition by controlling muramyl-L-alanine concentration and uses thereof.08-01-2013
20130196368Electrophysiological Recording System and Methods of Using Same - Electrophysiological recording systems for analysis of cultured cells include an recording device and a housing for supporting the electrophysiological recording device. The device has a porous membrane with top and bottom surfaces and a plurality of pores extending between the top and bottom surfaces. The porous membrane defines a first cell culture region disposed on the top surface and an opposed second cell culture region disposed on the bottom surface. The electrophysiological recording device has a plurality of electrodes positioned on the porous membrane in between a first insulation layer and a second insulation layer. The plurality of electrodes extend to the first cell culture region of the porous membrane. Recording ends of the electrodes measure electrical properties of cells cultured within the first and second cell culture regions, while the contact ends of the electrodes are positioned in electrical communication with data acquisition equipment.08-01-2013
20120295299Method and apparatus for rapidly analyzing microorganisms using petri plates - A growth plate or Petri plate and a method for detection and identification of microorganisms by specific or non-specific staining of colonies or microcolonies is described. Analytical substances (chromogenic or fluorogenic substrates, biochemical dyes, dyes indicators of pH, antibiotics and other biological active substances) are applied to the lower surface of a nutrient agar layer. The substances diffuse in the agar, reach cells and react with them. Because colonies or microcolonies are not removed from the media, they retain their shape, color, size and other useful characteristics for analytical purposes.11-22-2012
20120058508DETECTOR FOR CHEMICAL COMPOUNDS - Systems and methods to analyze contaminants including a plurality of stages configured to detect contaminants in a sample, wherein the plurality of stages are configured to detect a plurality of contaminants at substantially the same time.03-08-2012
20130203107METHOD FOR MEASURING THE AREA OF A SAMPLE DISPOSED WITHIN AN ANALYSIS CHAMBER - A method for determining the area of an analysis chamber covered by a biologic fluid sample quiescently residing within the chamber is provided. The chamber has a first panel with an interior surface, and a second panel with an interior surface, both of which panels are transparent. The method includes the steps of: a) illuminating the sample residing within the analysis chamber at one or more wavelengths operable to highlight interfaces between the sample and air, and to highlight a constituent within the sample; b) imaging the sample along the one or more wavelengths, and producing image signals representative of the interaction of the one or more wavelengths with the sample; c) determining a location of at least one interface between the sample and air, using the image signals; d) determining a location of one or more constituents within the sample relative to the at least one sample-air interface using the image signals; and e) determining an area of the chamber containing the sample, using the location of the one or more constituents and the at least one sample-air interface.08-08-2013
20130203108METHOD FOR PRODUCING HIGH PURITY X-CHROMOSOME BEARING AND Y-CHROMOSOME BEARING POPULATIONS OF SPERMATOZOA - Isolated non-naturally occurring populations of spermatozoa (08-08-2013
20090311740COLORIMETRIC METHOD AND RELATIVE DEVICE FOR BACTERIAL LOAD DETECTION - A method for detecting a bacterial load comprising adding a sample for analysis to an analysis reagent in a sterilized reaction container, thermostatting the reaction container at a temperature of between 25 and 45° C., and verifying the change in colouring of the analysis reagent. The analysis reagent is an aqueous solution comprising amino acids chosen from the group consisting of meat peptones, vegetable peptones, casein hydrolysates, tryptose, tryptones and yeast extract; glucides chosen from monometric or oligomeric glucides metabolisable by micro-organisms; a buffer system suitable for maintaining the pH between 5.5 and 8.5; a redox indicator with potential between −250 and +250 mV and/or a pH indicator with colour change interval between pH 4.0 and pH 9.0; and a water-immiscible organic liquid compound having a lower density than water and suitable for separating the aqueous phase from a gaseous phase existing prior to the analysis or formed during the reaction.12-17-2009
20120088266BIOREACTOR PROCESS FOR PRODUCTION OF HYDROGEN FROM BIOMASS - This invention relates to bioreactor processes, particularly bioreactor processes for the production of hydrogen gas from biomass, more particularly to bioreactor processes for the production of hydrogen gas employing a mixed anaerobic thermophilic bacterial consortium during the anaerobic fermentation of biomass.04-12-2012
20120088265Mold Pathogen Detection Apparatus - The present invention is a mold pathogen detection apparatus in which an agar strip is exposed to the environment being tested for a period of time and then exposed to a non-UV light to determine the amount of light passing through the agar strip.04-12-2012
20120094326IMAGING AND EVALUATING EMBRYOS, OOCYTES, AND STEM CELLS - Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.04-19-2012
20120094325Microfluidic Cell Motility Assay - Certain isolated motile cells spontaneously migrate unidirectionally through a mechanically confined space, such as a microcapillary channel, in the absence of an external gradient (e.g., a chemical gradient). Assays and methods for detecting motile cells, and identifying chemical agents that inhibit cell migration, can include detecting the movement of motile cancer cells through a microcapillary channel.04-19-2012
20120094324SYSTEM AND METHOD FOR HIGH THROUGHPUT CELL ANALYSIS AND SORTING - A sorting methodology in which bulk analysis of samples that have a low probability of containing a rare particle is performed first. Only those samples in which the bulk analysis has detected one or more rare particles are subjected to a flow cytometry process to isolate the rare particle(s). In one embodiment, a microwell plate provides for high throughput sorting in an enclosed environment. The microwell plate comprises a plurality of wells and a microfluidic layer adapted for flow cytometry wherein particles from a given well are interrogated and sorted into other wells. The microfluidic layer advantageously includes fluid switches to permit bi-directional flow cytometry.04-19-2012
20130210067METHOD OF DETECTING A BIOLOGICAL ACTIVITY - The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.08-15-2013
20130210064MAGNETOTACTIC ALGAE AND METHODS OF USE - Disclosed herein are magnetotactic algae, such as algae cells that include magnetic nanoparticles. In some examples the magnetotactic algae express a nucleic acid molecule encoding a bacterial MagA ferrous transporter, a nucleic acid molecule encoding a bacterial Mms6 magnetite binding protein, or both. Also disclosed herein are methods for producing magnetotactic algae and methods of producing biofuel or magnetic nanoparticles utilizing magnetotactic algae. Further disclosed herein are methods of enriching a population of magnetotactic algae cells (for example, increasing the number of magnetotactic algae cells in a population of algae cells). In further embodiments, disclosed herein are methods of selecting a transformed algae cell.08-15-2013
20130210065TETRAZOLIUM COMPOUND FOR DETECTING MICROORGANISMS, REAGENT FOR DETECTING MICROORGANISMS AND METHOD FOR DETECTING MICROORGANISMS - The object of the present invention is to provide a compound used in a reagent for detecting microorganisms, by which microorganisms can be rapidly and reliably detected without requiring any special instrument, the reagent for detecting microorganisms, and a method for detecting microorganisms. The object can be achieved by a tetrazolium compound represented by general formula (I) below:08-15-2013
20130210066APPARATUS AND METHOD FOR EARLY DIAGNOSIS OF CELL DEATH - An apparatus for measuring through optical means temporally resolved, optical properties, and/or phenotypes, linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to assess cell viability or early cell death through rapid diagnostic.08-15-2013
20130210068SUBSTRATE USED FOR CELL MIGRATION ASSAYS AND METHOD FOR CELL MIGRATION ASSAYS - This invention provides a means for cell migration assays by regulating the cells adhered to a substrate so that they migrate to a given region, which is a substrate used for cell culture comprising: a base material comprising a conductive region and an insulating region provided thereon and cell-adhesive regions and non-cell-adhesive regions provided in the conductive region and the insulating region, respectively, wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region, and a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the insulating region.08-15-2013
20130210069BIOLOGICAL STERILIZATION INDICATOR SYSTEM AND METHOD - A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.08-15-2013

Patent applications in class Determining presence or kind of micro-organism; use of selective media

Patent applications in all subclasses Determining presence or kind of micro-organism; use of selective media