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Involving viable micro-organism

Subclass of:

435 - Chemistry: molecular biology and microbiology

435004000 - MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435034000 Determining presence or kind of micro-organism; use of selective media 431
435032000 Testing for antimicrobial activity of a material 68
435030000 Methods of sampling or inoculating or spreading a sample; methods of physically isolating an intact micro-organism 33
435031000 Testing for sterility condition 10
Entries
DocumentTitleDate
20100035294SYSTEM TO ASSESS THE BIOLOGICAL ACTIVITY OF CHEMOATTRACTANTS - The present invention relates to a system for measuring the biological activity of chemoattractants comprising at least two units separated by a semipermeable carrier wherein biologically active carbohydrate structures, preferably glycosaminoglycan (GAG) structures, are immobilized on the surface of said carrier. According to the invention, this system can be used for fast and economic measurement of the degree of cell mobility and chemotactic activity.02-11-2010
20130045499Compact Multifunctional Ligand to Enhance Colloidal Stability of Nanoparticles - A ligand design allows compact nanoparticle materials, such as quantum dots (QDs), with excellent colloidal stability over a wide range of pH and under high salt concentrations. Self-assembled biomolecular conjugates with QDs can be obtained which are stable in biological environments. Energy transfer with these ligands is maximized by minimizing distances between QDs/nanoparticles and donors/acceptors directly attached to the ligands or assembled on their surfaces.02-21-2013
20130045500CELL EVALUATION METHOD - The present invention provides a method of figuring out an effect of a medicine exerting on the same cell quantitatively over a time. A cell evaluation method includes a step of culturing cells on a cell-culture base material that is capable of culturing spheroids, and a step of measuring a change over a time in the number of the spheroids formed through the former step or a percentage of the spheroids formed through the former step relative to an entire cultured cell.02-21-2013
20110177544METHOD FOR DISTINGUISHING AND SORTING OF CELLS AND DEVICE THEREFOR - A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.07-21-2011
20110201044TUBERCULOSIS DIAGNOSTIC TEST - A method of diagnosing in a host infection by or exposure to a mycobacterium which expresses ESAT-6 comprising (i) contacting a population of T cells from the host with one or more peptides or analogues selected from the peptides represented by SEQ ID NO:1 to 11 and analogues thereof which can bind a T cell receptor which recognises any of the said peptides, and (ii) determining whether the T cells of said T cell population recognise the peptide(s) and/or analogue(s). The method may performed in vivo. Peptides and a kit which enable the method to be carried out are provided.08-18-2011
20090176267IN VITRO METHOD FOR THE IDENTIFICATION AND EARLY IDENTIFICATION AND FOR THE CONCOMITANT MONITORING OF THE THERAPY OF DRUG-AND ADDICTIVE SUBSTANCE-INDUCED LIVER DAMAGE - Disclosed is an in vitro method for the identification and the concomitant monitoring of the therapy and cure of drug-induced or addictive substance-induced liver damage, in which the occurrence of the human enzyme carbamoyl synthase 1 (CPS 1) or its concentration is determined in serum or plasma samples from patients who are being or have been treated with potentially liver-damaging drugs, or from people who take harmful stimulants and addictive substances or are exposed to hepatotoxic substances.07-09-2009
20090208998D/P CREATININE MARKER, METHOD OF DETERMINING D/P CREATININE, AND INTENDED USE THEREOF - A method of indirectly determining D/P creatinine for easily judging a peritoneal function is provided that does not require blood sampling or multiple recoveries of dialysis effluent. The method includes preparing a dialysis effluent obtained by peritoneal dialysis, measuring at least one protein marker selected from the group consisting of prealbumin, haptoglobin, alpha 1-microglobulin, C4, and total protein that are contained in the dialysis effluent, and determining D/P creatinine indirectly from the correlation equation that represents the relationship between the marker concentration and the D/P creatinine value that has been prepared beforehand. Furthermore, the PET category is determined indirectly from the D/P creatinine, which has been determined indirectly, based on the known reference value.08-20-2009
20100081160Method for Diagnosing Multiple Sclerosis - Disclosed is a method for diagnosing multiple sclerosis and more particularly to a method for diagnosing multiple sclerosis by measuring levels of antibodies to glycans in a biological sample.04-01-2010
20100021957FUNCTIONALIZED QUANTUM DOTS AND METHODS FOR PREPARING THEM - A quantum dot (QD) conjugate comprises a QD and a ligand conjugated with the QD, in which the ligand has at least one thiol and at least one other functional group. The QD conjugate may further comprise a bioactive agent covalently coupled to the ligand to form a bioactive agent-tagged QD conjugate. A method for preparing a QD conjugate comprises the steps of: (1) providing a solution comprising a QD encapsulated within a dendrimer; (2) adding into the solution a ligand; and (3) allowing an exchange between the ligand and the dendrimer for the QD to obtain a ligand-QD conjugate, in which the ligand is covalently conjugated to the surface of the QD. The method may further comprise the step of coupling the ligand-QD conjugate to a bioactive agent to obtain a bioactive agent-tagged ligand-QD conjugate.01-28-2010
20080261260USE OF GELSOLIN TO TREAT INFECTIONS - The invention relates to the use of gelsolin to treat infections and to monitor the treatment of infections. The invention also provides methods up-regulating interleukin expression and methods for down-regulating pro-inflammatory cytokine expression.10-23-2008
20100062480DEVICE, SYSTEM AND METHOD FOR STORING AND SORTING CELLULAR SAMPLES - The present invention discloses a sample sorter system adapted to receive and sort samples according to predetermined criteria. The sample sorter system comprises a receptacle that is adapted to receive and retain fluid and samples. The receptacle is operatively coupled with a drive and with a power source such that actuation of the drive causes the rotation of at least one circular component, which in turn causes the development of a flow regime in the receptacle such that the samples suspended in the fluid are conveyable along a closed path to at least one sample handling site that are positioned along said closed path.03-11-2010
20100028934PIPETTE TIP AND A METHOD FOR PIPETTING A CONGEALED BLOOD SAMPLE UTILIZING THE PIPETTE TIP - A pipette tip and a method for pipetting a congealed blood sample utilizing the pipette tip are disclosed. The pipette tip includes an attachment section configured to be attached to a dispensing device, a liquid containing section configured to receive at least part of an aspired liquid sample, and a liquid aspiration section that is connected to the liquid containing section. The liquid aspiration section has a distal end aspiration opening and at least one further aspiration opening in a side wall.02-04-2010
20090053748Cytochrome c protein and assay - The present invention relates to a a cytochrome c-reporter fusion protein construct comprising a modified cytochrome c protein which targets the mitochondria and has a reduced ability to induce apoptosis in a living cell. The invention also relates to nucleic acid constructs encoding such protein fusions and cells stably transfected with such constructs. The stably transfected cells of the invention can be used in assays to detect apoptosis.02-26-2009
20120183990MICROFLUIDIC SYSTEM AND METHOD FOR PRODUCING SAME - A closed microfluidic system is equipped with a carrier plate and a cover plate as well as wall regions arranged therebetween, which form a system of channels and/or cavities with an inner surface. Selected regions of the inner surface are selectively functionalized.07-19-2012
20120183989METHOD FOR CULTURE OF HEPATOCYTES - A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.07-19-2012
20120183987CO-CULTURE BIOREACTOR SYSTEM - Disclosed herein are bioreactor systems and methods of utilizing said systems.07-19-2012
20120183986Fluorescent lactone ion indicators and their applications - Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.07-19-2012
20120183985METHOD FOR THE MANUFACTURE OF MICROTISSUES FOR INDUCING THE GROWTH OF A HAIR FOLLICLE - Disclosed is a method for the manufacture of microtissues, comprising the steps of: providing a biomaterial substrate; simultaneously seeding a plurality of dermal papilla (DP) cells and keratinocytes on the substrate surface with a predetermined ratio and cellular density; co-culturing for a predetermined period; and carrying the keratinocytes to the substrate surface by the dermal papilla cells, aggregating and finally form a plurality of keratinocyte-dermal papilla cell microtissues, wherein the dermal papilla cells are located in a centre of the microtissue and the keratinocytes are sorted to a surface of the microtissue, and the keratinocytes are adult keratinocytes. The method can help to simply and economize the procedures for production of folliculoid microtissues with high-throughput. Once microtissues are transplanted to skin of subject, hair follicles can be regenerated.07-19-2012
20100159499NANOPARTICULATE CELL CULTURE SURFACE - A cell culture article including a substrate having nanoparticles on the substrate surface, the nanoparticle including: 06-24-2010
20100009402METHOD OF EVALUATING BREAST CANCER, BREAST CANCER-EVALUATING APPARATUS, BREAST CANCER-EVALUATING METHOD, BREAST CANCER-EVALUATING SYSTEM, BREAST CANCER-EVALUATING PROGRAM AND RECORDING MEDIUM - According to the method of evaluating breast cancer of the present invention, amino acid concentration data on the concentration value of amino acid in blood collected from a subject to be evaluated is measured, and a breast cancer state in the subject is evaluated based on the concentration value of at least one of Ser, Gln, Val, Cys, Orn, Arg, Ile and ABA contained in the measured amino acid concentration data of the subject.01-14-2010
20110300571Determination of the Liver Toxicity of an Agent - The present invention provides various biomarkers for hepatotoxicity and various methods of using the biomarkers Some of the biomarkers within the scope of this invention are cholate, glycochenodeoxycholate, glycocholate, taurine, 3-hyroxy-2-ethylpropionate, 4-imidazoleacetate, tyramine, anthranilate, 2′-deoxycytidine, N-acetyl aspartate (NAA), beta-hydroxy-hexanoate, and sarcosine (N-methylglycine) The methods of using the biomarkers include exposing a first hepatocyte culture to a test agent and comparing the levels of the one or more biomarkers obtained in the first hepatocyte culture to the levels of the one or more biomarkers obtained in a second hepatocyte culture without the test agent, where differential levels of the one or more biomarkers in the first hepatocyte culture as compared to the levels in the second hepatocyte culture is indicative of the test agent being a hepatotoxicant.12-08-2011
20100129846Isoform specificities of blood serum proteins and their use as differentially expressed protein biomarkers for diagnosis of breast cancer - The present invention discloses twenty two 22 protein biomarkers of breast cancer. More specifically, the present invention discloses the identities, specificities, and uses of up to twenty two (22) protein biomarkers in blood serum for distinguishing between patients with earlier and later stages of breast cancer, patients with benign breast diseases or abnormalities, and normal individuals lacking breast abnormalities. More specifically, the present invention relates to specificities of isoforms of up to 22 protein biomarkers in blood serum for distinguishing between patients with earlier and later stages of breast cancer, patients with benign breast diseases or abnormalities, and normal individuals lacking breast abnormalities.05-27-2010
20090142795DEVICE AND METHOD OF MAINTAINING SPERM MOTILITY IN A CAPILLARY-LOADED CHAMBER - The invention relates, generally, to a method of removing sharp edges from a microscope coverslip comprising grinding down the edges and polishing the edges The invention also relates to a device for determining cell motility comprising a slide, a coverslip, comprising at least one edge that has been smoothed and a chamber, created by the slide and the coverslip and which is tangential to the coverslip, such that motile cells entering the chamber are substantially undamaged. The invention also relates to a method for using the device to determine cell motility.06-04-2009
20090191583CLOSTRIDIAL TOXIN ACTIVITY ASSAYS - Compositions useful for detecting Clostridial toxin activity comprising a cell that contains an exogenous Clostridial toxin substrate comprises a fluorescent member, a membrane targeting domain and a Clostridial toxin recognition sequence comprising a cleavage site, where the cleavage site intervenes between said fluorescent member and said membrane localization domain and methods useful for determining Clostridial toxin activity using such Clostridial toxin substrates.07-30-2009
20110195446METHOD AND KIT FOR SEMEN DIAGNOSIS THROUGH COLOR CHANGES IN METHYLENE BLUE AND SEMEN QUALITY EVALUATION USING SAME - The present invention relates to a method and a kit for diagnosis of semen quality (motility and concentration) through observation of color change visible with the naked eye in a solution combining methylene blue and sperm. The invention comprises a method for semen diagnosis using color coding (with a standard color table) charting the various colors the semen may change into, and a diagnostic it for testing semen using said method. When combined with methylene blue, healthier and more active sperm fades the original blue color more rapidly due to respiration activity by the sperm, which allows for semen quality evaluation. The above principle are adapted to kit from inside a tube which encases a methylene blue solution into which sample semen is introduced for easy evaluation.08-11-2011
20110195445METHODS AND SYSTEMS FOR FLUID EXAMINATION AND REMEDIATION - A system for in situ monitoring within a specified environment includes a housing with an intake inserted into the environment. A plurality of pumps are included in the housing with a number of test beds, each being separately coupled to one of the number of pumps, where each of the number of test beds holds material and where each of the plurality of pumps operate to separately push fluid through a coupled test bed. An effluent storage device is disposed to receive effluent from the number of test beds.08-11-2011
20100081162METHOD OF EVALUATING ANTIWRINKLE SUBSTANCE AND METHOD OF ASSESSING THE SKIN - It is intended to provide a method whereby the skin conditions (for example, loss in the skin elasticity, wrinkle formation possibility, insufficient tightness in dermal collagen fiber bundles, or the like) can be exactly and conveniently assessed. It is also intended to provide a method whereby the wrinkle-improvement effect of a test substance can be exactly and conveniently evaluated. The skin conditions are assessed by using the expression amount of an adhesion factor in skin cells as an indication. The wrinkle reducing effect of a test substance is evaluated by using the expression amount of an adhesion factor in skin cells in the presence of the test substance as an indication.04-01-2010
20100081161REAGENT FOR DILUTING BLOOD SAMPLE AND METHOD FOR MEASURING MEAN CORPUSCULAR VOLUME BY USING THE SAME - The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm/kg, as well as a method for measuring the mean corpuscular volume of a blood sample.04-01-2010
20090035803METHOD FOR STABILIZATION OF PEPTIDES IN A BIOLOGICAL SAMPLE - The present invention provides a simple method for the stabilization of a peptide in a biological sample and a reagent for the stabilization, a simple method for the preservation of a biological sample comprising a peptide and a reagent for the preservation, and a method for the accurate measurement of a peptide in a biological sample and a reagent for the measurement. Addition of a saccharide to a biological sample enables the stabilization of a peptide in the biological sample, the preservation of the biological sample comprising a peptide in a stable condition and the accurate measurement of a peptide in the biological sample. As the present invention can stabilize a peptide in a biological sample collected in the clinical examination, the peptide as a marker in the biological sample can be measured accurately in the clinical examination.02-05-2009
20090311734Laser Illumination System in Fluorescent Microscopy - Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Time Delay Integration imaging (TDI). Incorporation of TDI technology into image cytometry analysis, like CellTracks®, makes it possible to image moving objects with very high sensitivity and signal-to-noise ratios. Implementation of TDI camera technology with dual excitation and multispectral imaging of enriched rare cells provides a rapid system for detection, enumeration, differentiation and characterization of imaged rare cells on the basis of size, morphology and immunophenotype.12-17-2009
20090104642NOVEL UBIQUITIN LIGASES AS THERAPEUTIC TARGETS - The present invention relates to the discovery and characterization of activity of Fbp1, a substrate-targeting ubiquitin ligase subunit. The invention encompasses interactions between Fbp1 and its substrates, including Fbp5, β-Catenin, and IκBα. The invention also encompasses interactions between the Fbp1 isoform β-Trcp2 and its substrates, including Fbp5, b-Catenin, and IκBα. The present invention relates to screening assays that use Fbp1 and/or β-Trcp2 to identify potential therapeutic agents such as small molecules, compounds or derivatives which modulate Fbp1 and/or β-Trcp2 activity for the treatment of proliferative and differentiative disorders, including infertility, cancer, major opportunistic infections, immune disorders, certain cardiovascular diseases, and inflammatory disorders. The invention also encompasses methods to diagnose and treat Fbp1-related infertility disorders. The invention further encompasses therapeutic protocols and pharmaceutical compositions designed to target Fbp1 and its substrates for the treatment of infertility.04-23-2009
20100047842NOVEL TOXICITY ASSAY BASED ON HUMAN BLASTOCYST-DERIVED STEM CELLS AND PROGENITOR CELLS - An in vitro toxicity assay based on human blastocyst-derived stem cells for the detection of toxicity in the human species is provided, which enables novel detection of in vitro human toxicity for a substance and/or more efficiently detects human toxicity compared to non-human assays. Furthermore, the detection of toxicity for substances is enabled, which is known to display inter-species differences and the toxic effect was not detectable by toxicological tests in mice.02-25-2010
20120178122APPARATUS AND METHOD FOR ANALYZING BACTERIA - An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each particle contained in the analytic sample and an analyzing section for detecting Gram-positive bacteria contained on the basis of the detected optical information. A method for analyzing bacteria is also described.07-12-2012
20120178120MICROPLATE - The present invention relates to a microplate, or microtitration plate, having an invagination consisting of a continuous peripheral channel making it possible to add thereto, in a single introduction step, a liquid acting as an “evaporation curtain”. The invention is also directed to a device comprising such a microplate, to a manufacturing method and to use of such a microplate.07-12-2012
20100120077MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS - The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.05-13-2010
20100120081HIGH SENSITIVITY PARAMETERS FOR THE DETECTION OF VITAMIN B12 AND/OR FOLATE DEFICIENCIES AND METHODS OF USE - Described herein are high sensitivity parameters useful for the detection of vitamin B12 and/or folate deficiencies. Methods of determining susceptibility for vitamin B12 and/or folate deficiency in a subject are also provided. Methods of determining the progress and assessment of treatment of these deficiencies are provided.05-13-2010
20100120080CANCER DIAGNOSIS USING KI-67 - The present invention provides methods and compositions for detection, diagnosis, prognosis, monitoring treatment and monitoring progression of cancer by detecting the level of Ki-67 protein in an individual.05-13-2010
20100120078Urine Stabilization System - The present disclosure relates, according to some embodiments, to a method of stabilizing a molecule (e.g., a biomolecule) in a bodily fluid comprising: (1) providing a stabilizing solution comprising: (a) an amount of a divalent metal chelator selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and salts thereof in the range of from about 0.001 M to about 2 M; and (b) an amount of at least one chelator enhancing component selected from the group consisting of lithium chloride, guanidinium chloride, guanidinium thiocyanate, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1 M to about 10 M; and (2) adding the stabilizing solution to the bodily fluid, thus stabilizing the molecule. A biomolecule may be selected from a nitrite, a carbohydrate, a ketone, a globin, a bilirubin, a lipid, and combinations thereof in some embodiments.05-13-2010
20130078666SCAFFOLD-BASED ORGANOTYPIC CULTURE FOR THE LONG-TERM CULTIVATION OF HUMAN EPIDERMAL STEM CELLS - The present invention relates to organotypic cultures of epidermal cells and the use thereof for the screening of pharmaceutical and cosmetic agents. Specifically, means for the improvement of the long-term stability of such cultures are disclosed. Thus, the present invention contemplates a skin equivalent comprising (a) a dermal equivalent comprising a matrix comprising nonwoven viscose fabric and fibroblasts and (b) keratinocytes. Moreover, the present invention contemplates a method for manufacturing the skin equivalent and a method for screening agents capable of influencing skin, such as a therapeutic or cosmetic agent.03-28-2013
20130078665Test strip, a test kit and a method for detection of endotoxin in food - A home test strip for detection of endotoxin in food, the test strip includes an amebocyte lysate component, a chromogenic substrate component, and a carrier matrix. The amebocyte lysate component and the chromogenic substrate component are located in close proximity to each other and further incorporated within the carrier matrix. A home test kit for detection of endotoxin in food is also provided, which includes a sample holding container for collecting and holding a sample, a test strip, a standard colour scale. A method for detection of endotoxin in food is further provided, which includes the steps of preparing a food sample, placing a test strip in the food sample, waiting for an instructed time prior to removing the test strip, observing the formation of any colour, and matching the intensity of colour formed against a standard colour scale for deciding safety of food for consumption.03-28-2013
20100112629CELL BLOCK CASSETTE DEVICE - A tissue cassette assembly includes a housing having a recess formed therein, and a compressible reservoir disposed partially or wholly inside of, or otherwise attached in fluid communication with, the housing recess, the compressible reservoir containing a tissue embedding material. The tissue cassette further includes a port disposed in the housing, the port in fluid communication with the compressible reservoir at one end and terminating in a sample cavity at another end. During operation, the compressible reservoir is compressed or squeezed to release the tissue embedding material into the sample cavity containing the biological sample.05-06-2010
20100112628METHODS AND ASSAYS FOR DETECTING AND QUANTIFYING PURE SUBPOPULATIONS OF WHITE BLOOD CELLS IN IMMUNE SYSTEM DISORDERS - Methods for detecting nonactivated basophils in a whole blood sample obtained from a normal healthy subject, methods for determining a subject's susceptibility to an allergic reaction to an allergen, where the subject has no known allergy to the allergen, methods for measuring a response to challenge with a potential allergen in a whole blood sample obtained from a subject with known allergic reactivity to allergens other than the potential allergen; and an in vitro system for reliable detection or quantification of a specific white blood cell population in a whole blood sample are described.05-06-2010
20100075367Lung cancer detection by optical analysis of body fluids - The method for lung cancer detection by optical analysis of body fluids relates to analyzing samples of blood, urine and sputum by fluorescence spectroscopy in order to detect the presence of naturally occurring molecules in the fluids that serve as biomarkers indicative of cancer in the human body. The analysis can be carried out based on fluorescence emission spectra, fluorescence excitation spectra and synchronous (emission and excitation) spectra of bio-samples. The early detection and diagnosis of lung cancer may be made by comparison of ratios of fluorescence emissions and/or excitation intensities of tryptophan, tyrosine, elastin, collagen, bile pigments, NADPH, flavins and various species of porphyrins.03-25-2010
20100075366Methods for identifying stem cells by detecting fluorescence of cells and syncytia - Methods are disclosed for the characterization of the stage of development or pathology of a tissue sample, and for identifying pluripotent metakaryotic stem cells, comprising detecting fluorescence of cells and syncytia in fixed samples treated with a non-fluorescent Schiff's base reagent in the absence of extraneous or exogenously added fluorescent dyes.03-25-2010
20100075365PERINUCLEOLAR COMPARTMENT AS A CANCER MARKER - The present invention relates to compositions and methods for cancer diagnostics, prognostics and predictions, including but not limited to, cancer markers. In particular, the present invention provides perinucleolar compartments and their resident molecules as cancer markers.03-25-2010
20100075363Recombinant Yeast Strains Expressing Tethered Cellulase Enzymes - Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.03-25-2010
20100075362DEVICE AND METHOD FOR GROWING AND ANALYZING CELLS - A device and method for analyzing cells includes a housing with a chamber, a barrier supported by a frame disposed within the chamber, and a plate arranged at a bottom surface of the housing interior of the chamber. The plate is adapted to receive and sustain cells and the barrier separates the plate into at least two contiguous separate areas. In some embodiments, a thin rubber strip is arranged at the bottom edge of the barrier, which facilitates control of the area in which each cell type is grown, the size of the gap between the cells, and helps prevents over growth of the two cell types on to each other.03-25-2010
20100075360UV BASED CELL VIABILITY AS AN INDICATOR OF SUN PROTECTION FACTOR AND METHODS OF MEASUREMENT THEREOF - Disclosed is a simple method of determining SPF based on the staining pattern of viable cells like mouse fibroblasts and human keratinocytes after exposure to ultraviolet radiations. The method provides results comparable to the standard methods of SPF measurement using human subjects.03-25-2010
20100075364METHOD FOR SELECTING MAMMAL TRANSFORMED CELLS - It is an object of the present invention to provide a method for objectively selecting transformed mammalian cells from a state in which normal mammalian cells coexist with the transformed mammalian cells. The present invention provides a method for selecting transformed mammalian cells, which comprises treating a cell mixture comprising transformed mammalian cells and non-transformed mammalian cells with a solution containing active oxygen, so as to allow the transformed mammalian cells to selectively survive.03-25-2010
20100028928Device for High-Throughput Stimulation, Immunostaining, and Visualizaion of Single Cells - Cell stimulation, staining, and visualization are common techniques in both clinical and research settings. The invention is directed to microfluidic devices for in situ cell stimulation, staining, and/or visualization, and related methods for applying one or more stimuli to the cells, and fixing and staining of cells in situ. The device allows for high-throughput screening of living cells using a minimal quantity of reagents where the fate of individual cells can be followed over time.02-04-2010
20120244568LABEL-FREE RIGID CELL ASSAY METHOD - A label-free cell assay method including: 09-27-2012
20090053757Screening Method For Selecting Plants That Show A Reduced Wound-Induced Surface Discolouration - Provided is a method for screening a population of plants or plant parts for the presence of individuals showing reduced discolouration compared to a control plant or plant part. The method comprises providing a population of plants or plant parts, optionally creating a wound surface, and incubating the plant, plant parts, or wound surfaces created thereon to allow for discolouration. The discolouration is compared to that of control plants, plant parts, or wound surfaces, and plants or plant parts showing no discolouration or reduced discolouration, compared to control plants or plant parts are identified. Suitably, the discolouration is wound-induced discolouration.02-26-2009
20130084592METHODS FOR CORRECTING ASSAY MEASUREMENTS - Methods are disclosed for correcting an assay measurement for determining a concentration of an analyte in a sample suspected of containing the analyte. An assay signal is measured at a first wavelength corresponding to the analyte in the sample and an assay signal is measured at a second wavelength corresponding to background that is multiplied by a correction factor. An assay signal value is determined by subtracting assay signal at the second wavelength times the correction factor from assay signal at the first wavelength. The assay signal value is related to the amount of the analyte in the sample.04-04-2013
20090191584Transcriptional repression of sodium-iodide symporter in thyroid carcinoma - The present disclosure relates to a sodium iodide symporter (NIS)-repressor binding site (NRBS) consisting of a DNA molecule spanning from −645 to −605 nucleotides (SEQ ID NO:4) or from −648 to −620 nucleotides (SEQ ID NO:5) upstream from the translation start site of human NIS gene. The disclosure further relates to a method of restoring iodide transport to a human thyroid carcinoma cell, including: the steps of: i) contacting the cell expressing and forming a NIS repressor protein complex capable of binding to the NRBS of the disclosure with a modulator of said complex, and ii) administering to the cell radiolabeled iodide.07-30-2009
20130084595PLATFORM COMPRISING AN ORGANIC FIELD-EFFECT TRANSISTOR FOR BIOLOGICAL AND MEDICAL APPLICATIONS - The present invention relates to a device comprising an organic field effect transistor (OFET) with charge injecting contacts containing a semiconductor layer formed by a perylene derivative, to uses of said device as a medical sensor and/or as a medical cell stimulator and to methods of stimulating and/or monitoring biological cellular activity by using said device.04-04-2013
20130084594DRUG SUSCEPTIBILITY USING RARE CELL DETECTION SYSTEM - Methods for determining the efficacy of a given drug for a specific patient with cancer in vitro prior to, or after, the initiation of treatment of the patient are disclosed. Blood from the cancer patient is separated into an assay test tube and a control test tube. The blood in the assay test tube is exposed to a cancer drug. The two test tubes are then visually examined and compared to determine the effect of the cancer drug on cancer cells, other rare cells in the blood, or on normal constituents of the blood of a cancer patient.04-04-2013
20130084593RATIONALLY DESIGNED MEDIA FOR CELL CULTURE - This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.04-04-2013
20130084591ANALYTICAL TEST STRIP WITH ISOLATED BODILY FLUID PHASE-SHIFT AND ANALYTE DETERMINATION SAMPLE CHAMBERS - An analytical test strip (“ATS”) for use with a hand-held test meter in the determination of an analyte in a bodily fluid sample includes an electrically insulting substrate, a first patterned conductor layer disposed on the electrically insulating substrate and having a working electrode and a reference electrode. The ATS also includes an enzymatic reagent layer disposed on the working electrode, a first patterned spacer layer disposed over the first patterned conductor layer and defining both a first sample-receiving channel and an analyte determination sample chamber within the ATS, and a second patterned spacer layer disposed over the first patterned spacer layer and defining at least a second sample-receiving channel. The ATS further includes a bodily fluid phase-shift sample chamber in fluidic communication with the second sample-receiving channel. The first sample-receiving channel and analyte determination sample chamber are isolated from the second sample-receiving channel and bodily fluid phase-shift sample chamber.04-04-2013
20130034874AUTOMATIC PROCESS AND AUTOMATED DEVICE FOR PREPARING AND ANALYSING A PLURALITY OF CELL SUSPENSIONS - The present invention relates to a process for preparing and analysing a plurality of cell suspensions (02-07-2013
20090253162METHOD AND APPARATUS FOR ANALYZING SKIN AND HAIR - The present invention includes compositions, methods, and systems for the analysis of skin and hair conditions. The system includes a method and apparatus for analyzing skin and hair samples by taking a sample, identifying desired components of the sample, obtaining an image electronically, storing the image, and analyzing the image utilizing analysis software.10-08-2009
20130040335MULTI-SCALE WRINKLES FOR FUNCTIONAL ALIGNMENT OF STEM CELLS AND CARDIAC DERIVATIVES - Provided are methods of preparing a biocompatible textured surface on a thermoplastic material comprising treating the material with a plasma and subsequently shrinking the substrate to induce formation of textures. The textured surfaces are useful in one aspect, to align cells such as stem cells.02-14-2013
20130040334BIOCHIP ASSEMBLY AND ASSAY METHOD THEREOF - The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.02-14-2013
20130040331LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS - Disclosed are methods to classify human ether-à-go-go related gene (hERG) ion channel modulators using label-free biosensors. Disclosed is the use of label-free resonant waveguide grating (RWG) biosensors to reveal the patterns of the DMR signals of hERG modulators across three types of cell lines (a native cell line endogenously expressing hERG, a native cell line without hERG and its engineered cell line stably expressed hERG), as well as the corresponding modulation index of the modulators molecules against a hERG activator acting on a panel of markers/cells, particularly the known hERG activator mallotoxin DMR signals in the two hERG expressing cell lines.02-14-2013
20130040330METHOD FOR DIFFERENTIATION INDUCTION IN CULTURED STEM CELLS - Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.02-14-2013
20130040332METHOD FOR ASSAYING CELL MOVEMENT - The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.02-14-2013
20130040333ARRANGEMENT FOR DETECTION OF HEMOLYSIS - The present invention relates to a device for visual detection of hemolysis in a whole blood sample, comprising at least one visible detection compartment and a transfer passage connected to said visible detection compartment, said transfer passage being arranged to permit transfer of a volume of plasma from said sample to said detection compartment and wherein said transfer passage further is arranged with a separation device (02-14-2013
20100099132BACTERIAL GROWTH INDUCER - The present invention relates to methods of preparing bacterial growth inducers, and in particular to novel bacterial growth inducers/resuscitators prepared by such methods. The invention extends to various applications of such growth inducers, for example in clinical and environmental diagnostics, in reviving not immediately culturable (NIC) bacteria, and in the analyses of microbial populations in blood, food and soil samples.04-22-2010
20100099129Transcobalamin II assay method - An assay method for determining transcobalamin saturation wherein a transcobalamin containing liquid sample is contacted with a porous substrate with immobilized thereon a transcobalamin immobilizing ligand and with a reporter-labelled transcobalamin binding partner and wherein signals from reporter labels which become immobilized on said substrate are detected, characterised in that one of said ligand or said binding partner comprises a first ligand or binding partner capable of specific binding to holo transcobalamin and a second ligand or binding partner capable of binding to apo transcobalamin or to holo and apo transcobalamin.04-22-2010
20090162889GENE REPORTER ASSAY, KIT, AND CELLS FOR DETERMINING THE PRESENCE AND/OR THE LEVEL OF A MOLECULE THAT ACTIVATES SIGNAL TRANSDUCTION ACTIVITY OF A CELL SURFACE PROTEIN - The present invention relates to a commercializable cell and to a gene reporter assay method and a kit which use this cell to determine the presence and/or the level of a molecule that activates signal transduction activity of a cell surface protein. This cell is treated in such a manner that it will have a sufficiently long shelf life for its intended purpose, whereupon at the end of its useful shelf life or at the end of its use, i.e., in an assay, the cell undergoes cellular death.06-25-2009
20080293088Two-photon probe for real-time monitoring of intracellular magnesium ions, method for preparing the two-photon probe and method for real-time monitoring of intracellular magnesium ions using the two-photon probe - A two-photon probe for real-time monitoring of intracellular magnesium ions is provided. Specifically, the two-photon probe is represented by Formula 1:11-27-2008
20120264161MHC PEPTIDE COMPLEXES AND USES THEREOF IN INFECTIOUS DISEASES - Novel compounds carrying ligands capable of binding to counter receptors on relevant target cells are disclosed. The compounds possess a number of advantageous features, rendering them very suitable for a wide range of applications, including use as detection systems, detection of relevant target cells as well as a number of other methods. In particular, novel MHC complexes comprising one or more MHC molecules are disclosed. The affinity and specificity of the MHC-peptide complexes are surprisingly high. The possibility of presenting to the target cells a plurality of MHC-peptide complexes makes the MHC complexes according to the present invention an extremely powerful tool e.g. in the field of therapy and diagnosis. The invention generally relates to the field of therapy, including therapeutic methods and therapeutic compositions. Also comprised by the present invention is the sample-mounted use of MHC complexes and MHC multimers.10-18-2012
20120264160REVOLVING CELL CULTURE CARTRIDGE AND METHODS OF USE - A cell culture device and system can be used for high throughput biological assays to study the biological effect of a test substance. The device and system can include a revolving cartridge having a body including a center aperture and two or more evenly spaced sample wells that are spaced apart from each adjacent sample well by at least the diameter of each sample well. Each sample well can be positioned radially equidistant from the center aperture. Each sample well can have a fluid permeable membrane base configured to fluidly couple a top surface and a bottom surface of the body.10-18-2012
20100323385NITROGEN INDEPENDENCE IDENTIFIES A HIGHLY MALIGNANT POPULATION OF TUMOR STEM CELLS - The present invention is directed to a method for isolating and establishing Growth Factor-Independent (GF-I) Tumor Stem Cells (TSCs) from tumor biopsies or tumor cell lines consisting in culturing cells in serum-free mitogen-free culture medium. The method discloses cell growth in a culture medium, which does neither comprise serum, nor EGF (Epidermal Growth factor) and FGF-2 (Fibroblast Growth Factor), nor both, nor EGF or FGF-2 derivatives with the same mitogenic characteristics of the parent molecules. According to a preferred embodiment, the method is directed to the isolation of Tumor stem cells (TSCs) from glioblastoma multiforme (GBM) or from other brain tumors or brain tumor cell lines. GF-Independent TSCs can be identified and expanded in vitro providing a homogeneous population of multipotent, self-renewing and highly tumorigenic Growth Factor-Independent TSCs, distinguishable from tumor stem cells derived with other methods, grown in parallel, for the above characteristics. The invention also encompasses therapeutic methods based on Tumor Stem Cells isolated as described.12-23-2010
20100323384LABELING OF HUMAN EPIDERMAL STEM CELLS - The present invention provides skin equivalents comprising human epidermal stem cells which are specifically labeled. The labeling is carried out with a marker capable of labeling slowly proliferating cells, e.g. iododeoxyuridine or PKH26. Particularly, the invention provides skin equivalents comprising labeled epidermal stem cells. The invention also provides corresponding uses and methods of using such cultures, e.g. in the fields of research and medical treatment of skin diseases.12-23-2010
20090155837Two-photon fluorescent probes for acidic vesicles in live cells and tissue and method of imaging acidic vesicles in live cells and tissue using the same - Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by06-18-2009
20090155836METHODS FOR TREATING HIV INFECTED SUBJECTS - Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.06-18-2009
20090155835DIAGNOSTIC METHOD AND PROGNOSTIC TOOL FOR RHEUMATOID ARTHRITIS - The invention relates to a diagnostic method and prognostic tool for rheumatoid arthritis and uses thereof.06-18-2009
20090155834METHODS FOR IDENTIFYING AGENTS WHICH ALTER HISTONE PROTEIN ACETYLATION, DECREASE AGING OR INCREASE LIFESPAN - Methods of identifying agents which alter the NAD-dependent acetylation status and mono-ADP-ribosylation of nuclear proteins are disclosed. The methods further include identifying agents which alter the life span or aging of a cell or an organism by determining the level of NAD-dependent acetylation and/or ADP ribosylation of a nuclear protein. The invention also relates to a mammalian Sir2 protein which acetylates or deacetylates nuclear proteins in a NAD-dependent manner and has mono-ADP-ribosyltransferase activity. Host cells producing the Sir2 protein and antibodies to the Sir2 protein are also provided.06-18-2009
20090155833SCREENING METHOD OF NESFATIN-1-ACTION REGULATING SUBSTANCE OR NESFATIN-1-LIKE ACTION SUBSTANCE WITH THE USE OF RECEPTOR PROTEIN SELECTED FROM THE GROUP CONSISTING OF GPR3, GPR6 AND GPR12 - An object of the present invention is to identify a Nesfatin-1 receptor, and to provide a method for screening or designing a Nesfatin-1-action regulating substance or a Nesfatin-1-like action substance using the Nesfatin-1 receptor.06-18-2009
20090155831CARDIOMYOCYTES AND METHODS OF PRODUCING AND PURIFYING CARDIOMYOCYTES - The invention provides methods for producing a culture of cardiomyocytes and cultures of cardiomyocytes. Exemplary methods of producing and cultures of cardiomyocytes include a population of cells including cells having spontaneous and periodic electrical activity, and/or including nodal, sino-atrial or pacemaker cells; immature cardiomyocytes (cardiomyoblasts); mature contractile cardiomyocytes; or a mixed population of two or more of such cells.06-18-2009
20090155830Composition and a kit for detecting early apoptosis in frozen umbilical cord and a method therefor - There are provided a composition and kit for detecting early apoptosis in cryopreserved umbilical cord blood stem cells, and a method therefor. According to the present invention, when the umbilical cord blood stem cells are cryopreserved and later used for cell therapy, the quality of umbilical cord blood is assessed and early apoptosis in the umbilical cord blood stem cells is detected. The obtained resulting data, which can be used as a quality reference for umbilical cord blood required for transplantation, reflect the engraftment levels after in vivo transplantation of the stem cells, and thus allow prediction of the engraftment levels from the results.06-18-2009
20100105101METHODS FOR THE DIAGNOSIS AND RISK ASSESSMENT OF PLASMALOGEN DEFICIENCY MEDIATED DISEASES OF AGING - The present invention relates to methods for the diagnosis and risk assessment of plasmalogen deficiency mediated diseases of aging. The present invention describes the relationship between plasmalogen biosynthesis dysfunction and the biochemical and clinical manifestations of age related disorders. Specifically the present invention describes an increased prevalence of colon cancer, prostate cancer, lung cancer, breast cancer, ovary cancer, kidney cancer, cognitive impairment and dementia in subjects suffering from adult onset plasmalogen biosynthesis disorder (AO-PBD).04-29-2010
20100105099TRI-FUNCTIONALIZED CRYPTOPHANE, SYNTHESIS AND USES THEREOF - This invention relates to biosensors with improved solubility and affinity to a noble element. Specifically, the invention relates to methods and systems for the detection of target entities using the signal observed in a noble element complexed to the biosensor.04-29-2010
20100105098Methods of Identifying Disease Biomarkers in the Lense of the Eye - The present invention relates to methods for the early diagnosing of an amyloid-related disorder or a predisposition thereto in a subject through the detection or monitoring of a metal-protein complex in the ocular lens, wherein said metal-protein complex comprises at least one amyloid protein.04-29-2010
20100105097TWO-PHOTON PROBE FOR REAL-TIME MONITORING OF INTRACELLULAR CALCIUM IONS, METHOD FOR PREPARING THE PROBE AND METHOD FOR REAL-TIME MONITORING OF INTRACELLULAR CALCIUM IONS USING THE PROBE, - A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20-5 O-fold TPEF enhancement in response to Ca2+, has a dissociation constant (Kdτp) of 0.14±0.02 to 0.25±0.03 μM, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 μm in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.04-29-2010
20100105100MULTIPOTENT/PLURIPOTENT CELLS AND METHODS - Described herein are multipotent stem cells, e.g., human and other mammalian pluripotent stem cells, and related methods.04-29-2010
20100105102DEVICE, METHOD AND APPARATUS FOR ANALYZING SKIN AND HAIR - The present invention includes compositions, methods, and systems for the analysis of skin and hair conditions. The system includes a method and apparatus for analyzing skin and hair samples by taking a sample, identifying desired components of the sample, obtaining an image electronically, storing the image, and analyzing the image using analysis software.04-29-2010
20100041089IMPROVED METHOD FOR DETECTING CHEMICAL SUBSTANCES - The present invention provides an assay method for conveniently and easily detecting existence or a quantity of a chemical substance in the environment without relying on expensive measurement devices used in fluorometry. More specifically, the present invention is characterized in that a transformed cell which is transformed by operably linking a polynucleotide encoding oxidoreductase at a downstream of a promoter gene for monitoring whose promoter activity varies with responding to a chemical substance is used.02-18-2010
20100041092DRUG CONTROLLED MOLECULAR TAGS - The disclosure provides method and compositions for visualizing protein turnover. In particular, the disclosure provides methods and compositions useful for measuring the age of particular proteins or the dynamics of localized protein translation.02-18-2010
20100041090EMBRYO QUALITY ASSESSMENT BASED ON BLASTOMERE DIVISION AND MOVEMENT - The invention concerns a system and method for determining embryo quality comprising monitoring the embryo for a time period, said time period having a length sufficient to comprise at least one cell division period and at least a part of an inter-division period, and determining the length of the at least one cell division period; and/or ii) determining the extent and/or spatial distribution of cellular or organelle movement during the cell division period; and/or iii) determining duration of an inter-division period; and/or iv) determining the extent and/or spatial distribution of cellular or organelle movement during the inter-division period thereby obtaining an embryo quality measure. Thus, the selection of optimal embryos to be implanted after in vitro fertilization (IVF) is facilitated based on the timing, duration, spatial distribution, and extent of observed cell divisions and associated cellular and organelle movement.02-18-2010
20100041093DEVICES AND METHOD FOR ELECTROPHYSICAL CELL ANALYSES - Disclosed is a liquid holder mechanism (02-18-2010
20100041091MICROFLUIDIC EMBEDDED POLYMER NEMS FORCE SENSORS - A method of screening one or more cells is described; the method includes: (i) providing one or more cells to a nanoelectromechanical system (NEMS) force sensor; (ii) applying at least one reagent to the one or more cells; and (iii) observing a response of the one or more cells to the reagent with the force sensor, thereby screening the one or more cells.02-18-2010
20100159498BLOOD ANALYZER WITH A BLOOD CELL SEDIMENTATION CONTROL MECHANISM AND METHOD OF USE - A blood analyzer having a blood cell sedimentation control mechanism is disclosed, which includes a cassette receiving interface including a cassette compartment and a blood sensor operable to detect a presence of blood in a disposable cassette removably disposed within the cassette compartment; a system control electrically connected to the blood sensor, and a blood measurement assembly connected to the system control and adapted to connect with the disposable cassette. The system control includes a time recording mechanism and a predetermined sedimentation time control criterion. Further disclosed is a method of controlling blood cell sedimentation during sample preparation process on the blood analyzer.06-24-2010
20120034641BIOLOGICAL INFORMATION ACQUISITION METHOD - A biological information acquisition method includes: measuring an amount of nicotinamide metabolite in a sample collectable from a living organism in a minimally invasive manner; and acquiring information concerning the living organism based on the measured amount of nicotinamide metabolite.02-09-2012
20090093013Single-Cell Label-Free Assay - The disclosure provides a system and method for characterizing a single live-cell response to a stimulus with a biosensor imaging system having cells immobilized on the biosensor at a resolution level of a single cell, as defined herein.04-09-2009
20100330608MOLECULAR TRANSPORTERS BASED ON SUGAR AND ITS ANALOGUES AND PROCESSES FOR THE PREPARATION THEREOF - The inventive molecular transporter compound shows significantly high permeability through a biological membrane such as a plasma membrane, nuclear membrane and blood-brain barrier, and accordingly, can be effectively used in delivering various biologically active molecules.12-30-2010
20100330604QUANTIFICATION OF CHANGES IN THE DEGREES OF ORDER OF CELLULAR AND VIRAL MEMBRANES AND APPLICATIONS TO DIAGNOSIS, TREATMENT AND DRUG SCREENING - A method for characterizing cell membrane order in a cell. The method includes: staining the cell with di-4-ANEPPDHQ to produce a stained cell; irradiating the stained cell with an excitation light, the excitation light being capable of inducing fluorescence in the di-4-ANEPPDHQ; measuring a fluorescence spectrum of the stained cell; and characterizing the cell membrane order by computing a spectral signature of the stained cell from the fluorescence spectrum, the spectral signature providing a character of the cell membrane order.12-30-2010
20130029369BIMODAL STAR POLYMER ARCHITECTURES AS FLUORESCENT AND MRI IMAGING REAGENTS - Disclosed are star polymers comprising a polymeric body having a core with a site-isolated chromophore and a plurality of polymer chains emanating from the core; and at least one chelating moiety bonded to at least one polymer chain. Also disclosed are bimodal contrast agents derived from star polymers and further comprising at least one metal chelated by the at least one chelating moiety. Also disclosed are methods of making and using same. Also disclosed are imaging methods employing the disclosed star polymers and/or bimodal contrast agents. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.01-31-2013
20130029370System for Purifying Certain Cell Populations in Blood or Bone Marrow by Depleting Others - An apparatus and method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed.01-31-2013
20090011454Fluorescent Sensors for Cellular Amines - The invention provides compounds of formulas I, II, and III, methods of making them, and methods of their use. The compounds of the invention can be used as fluorescent sensors, for example, to detect an amine-containing analyte in a biological sample. The compounds can be selective for one type of amine over others and the amount of fluorescence can be correlated with the concentration of the amine in the sample.01-08-2009
20090269795Mutant alpha4betadelta GABAA receptor and methods of treating anxiety or irritability - The present invention provides methods for treating anxiety or irritability in a subject. The methods comprise administering to the subject an effective amount of an antagonist of allopregnanolone (THP), or a regulator which decreases expression of the alpha 4 subunit of GABA such as gabadoxbol (THIP), or a vector comprising an isolated nucleic acid molecule encoding a mutant alpha 4 subunit GABA10-29-2009
20100068747Generic Assay for Monitoring Endocytosis - A method for monitoring the internalisation of a cell surface molecule of interest is provided utilizing a detectable lectin.03-18-2010
20090123961MICROFLUIDIC DEVICE HAVING STABLE STATIC GRADIENT FOR ANALYZING CHEMOTAXIS - A microfluidic method and device for testing and analyzing chemotaxis by providing a stable, static fluid gradient. The device includes a sink reservoir for receiving biological cellular material and a source reservoir for receiving a chemoattractant. The biological cellular material migrates through a low fluid volume microfluidic gradient channel located between the source and sink reservoirs. The fluid in the gradient channel is static and stable due to a high fluid volume closed circuit bypass microfluidic channel also in fluid communication with the source and sink reservoirs, whereby the bypass channel relieves any pressure differential imparted across the gradient channel.05-14-2009
20090123959MICROORGANISM DISCRIMINATOR AND METHOD - A microorganism discriminator is disclosed, including a housing to incubate a sample in low-light conditions; a illuminator to irradiate the sample with a monochromatic blue light; an injector disposed in the housing, to deliver a viability discriminating dye to the sample; and a base connected to the housing and the illuminator, to transport the sample to the housing and to the illuminator. A method of discriminating viable microorganisms in a sample is disclosed, the method including: applying a sample to a filter; applying a viability discriminating dye to the filter, in a low-light environment; incubating the sample in the low-light environment; illuminating the filter with monochromatic blue light; and performing quantitative polymerase chain reaction (QPCR) on the sample.05-14-2009
20090123958Laboratory Devices, Methods and Systems Employing Acoustic Ejection Devices - A method for performing chemically analyzing samples (e.g. bodily fluids) dispensed with an acoustic ejection device is disclosed. A method preparing a sample for analysis by centrifuging the sample in a sample collection device in fluid communication with an acoustic ejection device is disclosed. A method for laboratory analysis of biological and/or other fluids, wherein a single electromechanical pump including an acoustic ejection device draws and ejects fluids is disclosed. A device for dispensing a fluid, where a ratio between a reservoir of the device and an ejection chamber is between 50 and 4,000 is disclosed. A system including a plurality of acoustic ejection devices in an environmentally enclosed housing is disclosed.05-14-2009
20110008822CASSETTE AND METHOD FOR PLANT SAMPLING - A method and storage medium (01-13-2011
20130089884Protein Modification from the Oxidation of Clickable Polyunsaturated Fatty Acid Analogs - Clickable polyunsaturated fatty acid analogs, methods of using these analogs and kits comprising these analogs.04-11-2013
20100136605SYSTEM FOR AND METHOD OF POSITIONING CELLS AND DETERMINING CELLULAR ACTIVITY THEREOF - A device for positioning at least one cell in at least one addressable position, the device comprising a substrate formed with at least one addressable pore and at least one channel embedded in the substrate and being in fluid communication with the at least one pore. The at least one pore and the at least one channel are designed and constructed such that an under-pressure formed in the at least one channel results in vacuum adherence of the at least one cell onto the at least one pore, such that a single cell is vacuum adhered onto a single pore. In one embodiment, the substrate is a non-conductive substrate and is further formed with one or more electrode structures, where each of the electrode structures is positioned in one of the pores. In an additional embodiment the device is designed locatable onto an organ, such as a brain.06-03-2010
20090317858CELLULAR ASSAYS FOR SIGNALING RECEPTORS - The present invention provides cells and methods related to signaling receptors. The cells of the invention express the signaling receptors (e.g., in a constitutively active state). The cells are useful for analyzing the signaling receptors and their related pathways. The invention further provides methods for studying interactions of the signaling receptors and for small molecule screening, including high throughput methods. The invention further relates to expressing a signaling receptor (e.g., a GPCR) in a constitutively active state, even in the absence of the receptor's ligand. This allows for screening for inhibitors of the activated receptor's pathway without even knowing the ligand that activates the receptor, e.g., an orphan receptor. The invention further provides cell lines for expressing a signaling receptor in a constitutively active state. These cell lines are useful for high throughput screening assays of the invention.12-24-2009
20100267075MICROFLUIDIC LYSIS - The invention provides methods for isolating and identifying nucleated cells from biological samples using a microfluidic device.10-21-2010
20100129845Astaxanthine Biosynthesis in Eukaryotes - The invention relates to a DNA vector comprising (a) a DNA sequence coding for the phytoene desaturase protein that is modified in one position by an amino add exchange providing resistance, and (b) a multiple cloning site into which any DNA sequence to be cloned. The invention also relates to the use of said DNA vector for transforming enkaryotic cells, transformation methods, and transgenic plant cells produced in said manner.05-27-2010
20090042238Method of evaluating cell function, system for evaluating cell function, fluorescent microscope system, phototherapy method and phototherapy system - A proposition is to accurately evaluate a phototoxic property (cell function) concerning a living cell. To this end, an evaluating method of cell function includes the operations of dyeing a specific site of the living cell with a fluorescent dye, irradiating the living cell with light to measure changes in brightness of resulting fluorescence generated at an adjacent site of the specific site, and evaluating the phototoxic property based on the brightness changes. In the event of functional depression in the specific site, the fluorescent dye cannot be retained therein and is extravasated into the adjacent site through the membrane of the specific site. The present embodiment can measure the extent of this extravasation, making it possible to accurately evaluate the phototoxic property.02-12-2009
20090042236Method of identifying hydrogen evolving diazotrophic bacteria - MohC is required for expression of uptake hydrogenase in 02-12-2009
20090042234ULTRA SENSITIVE BIOSENSOR FOR DETECTING BIO-SIGNAL TRANSMITTERS, AND DETECTOR AND DETECTING METHOD USING THE SAME - The present invention relates to a novel use of a transfected cell as a biosensor, wherein the transfected cell expresses a receptor specific to a bio-signal transmitter, and a highly sensitive detector and a highly sensitive detecting method of a bio-signal transmitter using the biosensor. The bio-signal transmitter detecting technique according to the present invention has an advantage of being applied to a sniffer-patch method, and is capable of providing millisecond time resolution for detecting nanomolar levels of the bio-signal transmitter to be detected.02-12-2009
20090042233NOVEL PROTEIN MEMBER OF THE RAS/MAPK PATHWAY, ANTIBODIES THEREOF AND METHODS AND KITS OF USING SAME - A polypeptide comprising a SAM domain, the sequence of said SAM domain being as set forth in SEQ ID NO: 33.02-12-2009
20130089885CD49F PROMOTING PROLIFERATION, MULTIPOTENCY AND REPROGRAMMING OF ADULT STEM CELLS THROUGH PI3K/AKT/GSK3 PATHWAY - The present invention relates to a method for obtaining adult stem cells, which have a surface antigen of CD49f, excellent formation of spheres due to sphere formation and high expression of OCT4 and SOX2, from a cell source including stem cells, and a cell therapeutic agent containing adult stem cells obtained by the method or cells differentiated therefrom as an active ingredient.04-11-2013
20110003326MICROINJECTION APPARATUS AND METHOD - The present invention discloses a microinjection apparatus (01-06-2011
20090305332Method and Apparatus for Determining an Analyte in a Liquid Sample - A method for the determination of an analyte in a liquid sample, especially a body liquid sample, with the aid of an analytical apparatus. The apparatus receives a first measuring signal s12-10-2009
20090305330METHOD AND APPARATUS FOR THE DETECTION OF LIVING PHYTOPLANKTON CELLS IN WATER - The invention related to a method and an apparatus for detecting living phytoplankton cells and/or microorganisms in or out of water, particularly ballast water, bodies of water, sewage, or water in swimming and bathing devices. Said method is characterized by the following steps:—the variable fluorescence (Fv) is calculated by forming the difference between the maximum fluorescence (Fm) and the minimum fluorescence (Fo) in a measuring space or detecting part or all of the dynamic shape of a fluorescence induction curve in a measuring space, particularly measuring; and—calculating the number of living phytoplankton cells and/or microorganisms of a reference species in the measuring space in accordance with the variable fluorescence (Fv).12-10-2009
20090305329INTRACELLULAR TARGETING OF MOLECULES - The present invention provides methods for intracellular and/or nuclear targeting of an agent capable of specifically binding to syndecan-4. The present invention further provides methods for the modification of the intracellular and/or nuclear targeting of said agent, as well methods for identifying compounds capable of modifying the syndecan-4 delivery pathway. The present invention further provides experimental kits to perform the methods according to the invention.12-10-2009
20090305328METHOD OF IMPROVEMENT OF ORGANISMS USING PROFILING THE FLUX SUM OF METABOLITES - The present invention relates to a method for improving an organism through the profiling of flux sum of metabolites, and more particularly to a method for screening key metabolites, the method comprises: plotting a profile between objective functions based on useful substance formation rate as a main function through an algorithm perturbing other functions influencing the production of useful substance; determining the utilization (flux sum (Φ)) of all metabolites from the profile; and screening key metabolites, which show an increase in flux sum (Φ) with an increase in useful substance formation rate. The present invention also relates to a method for improving an organism producing a useful substance, the method comprises introducing and/or amplifying genes associated with the screened key metabolites or introducing the genes from the outside into the organism. According to the disclosed invention, the metabolic utilization (flux sum; Φ) of specific metabolites according to an increase in useful substance formation rate can be predicted, so that key metabolites in increasing the production of a useful substance can be screened. Also, it is possible to increase the production of a useful substance through the method of improving a target organism by introducing and/or amplifying genes associated with the screened metabolites or through the method of supplying the metabolites during the culture of the organism.12-10-2009
20090305326MICROFLUIDIC DEVICE AND METHOD FOR COUPLING DISCRETE MICROCHANNELS AND FOR CO-CULTURE - A microfluidic device and method is provided for coupling discrete channels and for co-culture. The microfluidic device includes first and second bodies. Each body has a bottom surface and defines a channel. The channel in each body includes an inlet and an outlet communicating with the bottom surface. A first fluid, such as a first cell suspension, is provided within the channel of the first body and a second fluid, such a second cell suspension, is provided within the channel of the second body. The first and second bodies are movable between a first position wherein the outlet of the channel of the first body is spaced from the inlet of the channel of the second body and a second position wherein the fluid at the outlet of the channel of the first body communicates with the fluid at the inlet of the channel of the second body.12-10-2009
20090305325Method for Preservation of Human Hematopoietic Stem or Progenitor Cells - Maintenance of quiescent hematopoietic stem and progenitor cells (HSPC) in culture without the addition of exogenous growth factors is an important aspect in clinical hematology. A recent report described the ability of Flt3 receptor-interacting lectin (FRIL) in the maintenance of cord blood (CB) derived progenitors in vitro. Since FRIL is a mannose binding lectin, the effectiveness of four such lectins of well-characterized specificities on the preservation of HSPC of CB origin have been examined. The HSPC preservation activity of lectins was assessed by in vitro colony forming unit (CFU) and long-term culture initiating cell (LTC-IC) assays. It was found that all four lectins had a HSPC preservation activity at least up to 30 days in culture without addition of exogenous growth factors. The results indicate that use of such lectins may provide a cost effective method of HSPC maintenance for clinical use.12-10-2009
20090305322Isotope Detection and Uses Thereof - A method is disclosed for measuring the hydrogen and/or oxygen isotope ration of intracellular water in 12-10-2009
20090305321Assays for Allosteric Modulators of G-Protein Coupled Receptors (GPCRs) - The present invention relates to G protein-coupled receptors (GPCRs) and allosteric modulators thereof. More specifically, the invention relates to allosteric modulators of GPCRs that interact at an intracellular binding site. It also relates to methods for designing or identifying small molecule allosteric modulators, including assays (such as competitive binding assays) and methods employing a homology model for the GPCR intracellular site.12-10-2009
20090305334METHOD FOR MAKING A BIOLOGICAL INDICATOR FOR USE WITH VAPOROUS MICROBIAL DEACTIVATING AGENTS - A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent.12-10-2009
20090305320Method of detecting activation of notch signal transmission system - The purpose of the present invention is to provide detection methods of Notch signaling activation for detecting the activation of the Notch signaling in living cells simply and conveniently. The expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene which is controlled by the wild-type Hes-1 gene promoter has been introduced is compared with the expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene controlled by a mutated Hes-1 gene promoter which is not controlled by an activated Notch protein has been introduced, and a transgenic cell in which a signal by the expression of Venus introduced by the vector having the wild-type Hes-1 promoter is observed and in which a signal by the expression of Venus introduced by the vector having the mutated Hes-1 gene promoter which is not controlled by the activated Notch protein is not observed is identified.12-10-2009
20090305319Apparatus and methods for monitoring the status of a metabolically active cell - An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.12-10-2009
20130071873ARRAY MICROINJECTION APPARATUSES AND METHODS - An array microinjection apparatus comprises a substrate (03-21-2013
20130071872DEVICE AND METHOD FOR SINGLE-USED MATRIX SAMPLING - A single-used matrix sampling device for the single-used, disposable or autoclavble packed-bed bioreactor is provided herein. The present invention includes a tube means and a matrix collecting means. The matrix collecting means configured for collecting the cell culture matrix includes a receptacle and a pulling means connected to the receptacle or the matrix collecting means could be scoop-like in configuration; the tube means is configured for preventing the matrix collecting means from being contaminated while mechanically or pneumatically sampling; and the tube means could be sealed to enclose the sampled matrix collecting means after sampling. A single-used matrix sampling method for a single-used, disposable or autoclavble packed-bed bioreactor is also provided. The present invention could be utilized to aseptically collect the cell culture matrix from the packed-bed bioreactor without requiring to face the risks of contamination.03-21-2013
20090104650Infectious diseases testing of menstrual fluid, endometrial/menstrual cells, amniotic fluid, umbilical cord blood or other samples - Methods are provided for obtaining and testing or analyzing a non-venous and non-arterial puncture human fluid or cell sample or human body fluid or cell sample to detect the presence of at least one infectious disease. The sample may be menstrual fluid, endometrial menstrual cells, umbilical cord blood, or amniotic fluid. Confirmatory testing of a corresponding arterial or venous blood sample for comparison to the test results for the non-venous and non-arterial human fluid or cell sample may be performed. The testing may comprise a screening test or a confirmatory test.04-23-2009
20090104648TRANSFORMED CELL WITH ENHANCED SENSITIVITY TO ANTIFUNGAL COMPOUND AND USE THEREOF - The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like.04-23-2009
20100273200In-Vitro Model of Blood-Brain Barrier, In-Vitro Model of Diseased Blood-Brain Barrier, and Drug Screening Method, Analysis Method for Functions of Diseased Blood-Brain Barrier, and Analysis Method for Pathogenesis Using the Same - It is intended to provide a screening system for a centrally acting drug transported across the blood-brain barrier, a drug acting on the blood-brain barrier itself, or a drug transferred into the brain without being expected to centrally act. Moreover, another object of the present invention is to achieve pathogenesis analysis study or the screening in a diseased state by applying various diseased environments to this screening system. The present invention provides an in-vitro model of blood-brain barrier obtained by using a three-dimensional culture apparatus comprising: a culture solution; a plate holding the culture solution; and a filter immersed in the culture solution and placed in no contact with the inside bottom of the plate, the filter having plural pores of 0.35 to 0.45 μm in diameter, and by comprising: seeding primary cultured brain capillary endothelial cells onto the upper surface of the filter; seeding primary cultured brain pericytes onto the under surface of the filter; seeding primary cultured astrocytes onto the inside surface of the plate; and coculturing these cells in a normal culture solution.10-28-2010
20090093012REACTION CASSETTE FOR MEASURING THE CONCENTRATION OF GLYCATED HEMOGLOBIN AND MEASURING METHOD THEREOF - A reaction cassette for a glycated hemoglobin meter and a measuring method thereof are provided. The reaction cassette for the glycated hemoglobin meter includes: a first zone receiving a first reagent and a blood sample; a second zone receiving a second reagent; a reaction zone in which the blood sample reacts with the first reagent, or through which the second reagent passes to react with a first blood sample mixture obtained by reacting the blood sample with the first reagent; and a measurement zone measuring an amount of total hemoglobin in the first blood sample mixture, or measuring an amount of glycated hemoglobin in a second blood sample mixture obtained by reacting the first blood sample mixture with the second reagent, wherein the blood sample, the first reagent, and the second reagent move between the reaction zone and the measurement zone according to a rotation angle of the reaction cassette when the reaction cassette is rotated. Therefore, since the reaction cassette rotates automatically, it is possible to measure the amount of glycated hemoglobin in a blood sample through simple manipulation and reduce a manufacturing time. Furthermore, since reagents are supplied to the reaction cassette from a separate reagent pack, it is possible to resolve storage and distribution problems of the reaction cassette, which occur when reagents are stored in the reaction cassette.04-09-2009
20130059322CELL CULTURE AND INVASION ASSAY METHOD AND SYSTEM - Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.03-07-2013
20110039296METHOD OF ATTACHING A CELL-OF-INTEREST TO A MICROTUBE - A method of attaching a cell or a membrane-coated particle-of-interest to a microtube is provided. The method comprising: co-electrospinning two polymeric solutions through co-axial capillaries, wherein a first polymeric solution of the two polymeric solutions is for forming a shell of the microtube and a second polymeric solution of the two polymeric solutions is for forming a coat over an internal surface of the shell, the first polymeric solution is selected solidifying faster than the second polymeric solution and a solvent of the second polymeric solution is selected incapable of dissolving the first polymeric solution and wherein the second polymeric solution comprises the cell or the membrane-coated particle-of-interest, thereby attaching the cell or the membrane-coated panicle-of-interest to the microtube. Also provided are microtubes with attached, entrapped or encapsulated cells or membrane-coated particles and methods of using same.02-17-2011
20110039295DISPLAY WITH ICONIC MARKERS FOR A METER - Meters, methods, and computer-readable media for determining the concentration of an analyte in a fluid sample are presented herein. One concept is directed to a meter for determining the concentration of an analyte in a fluid sample. The meter includes a housing configured to receive a test sensor carrying the fluid sample, and a processor configured to determine analyte concentration information from the fluid sample. A memory is coupled to the processor and configured to store the analyte concentration information. A display is coupled to the housing and configured to display the analyte concentration information and one or more iconic markers. Each iconic marker represents a respective state of the user. Each user state has a known affect on the analyte concentration information. An input device is coupled to the processor and configured to receive the user's selection from the iconic markers. The user's selection is stored by the memory.02-17-2011
20110059481METHOD AND APPARATUS FOR DETERMINING RED BLOOD CELL INDICES OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS - A method for the determination of the red blood cell indices including the volume, and hemoglobin content and concentration for individual red blood cells, as well as red blood cell population statistics, including total number of red blood cells present in the sample, and mean values for each of the aforementioned indices within a substantially undiluted blood sample is provided.03-10-2011
20110059479MODULATION OF THE INTEGRIN LINKED KINASE SIGNALING PATHWAY TO PROMOTE CARDIAC CELL PROLIFERATION AND SELF-RENEWAL - The invention relates to a process for identification, amplification and differentiation of cardiac stem cells by utilizing an ILK based protocol. The invention further relates to a process for post myocardial infarction (IVII) remodeling by way of the integrin linked kinase (ILK) signaling pathway. Still further, the invention relates to a process for affecting an ability to control and/or manipulate stem cell frequency, cellular fate, self-renewal, multilineage differentiation, and potential for oncogenesis in cardiac tissue derived from embryonic stem cells, fetal tissue or adult tissue comprising modulation of ILK signaling amplification whereby stem cell renewal and expansion results.03-10-2011
20090286275Two-photon probe for real-time monitoring of intracellular free zinc ions, method for preparing the probe and method for real-time monitoring of intracellular free zinc ions using the probe - A two-photon probe for real-time monitoring of intracellular free zinc ions is provided. The two-photon probe is represented by Formula 1:11-19-2009
20110014642METHOD OF SCREENING FOR COMPOUNDS THAT CAN BE USED FOR THE TREATMENT OF RESPIRATORY CONDITIONS - The present invention relates to the use of a screening method for identifying candidate molecules that can be used for the treatment of respiratory conditions in a mammal, wherein said screening method comprises a step which comprises determining whether the functional activity of a TASK-2 polypeptide in the presence of a test molecule is decreased or eliminated compared with the functional activity of said TASK-2 polypeptide in the absence of said test molecule, the test molecule being considered to be a candidate molecule when it decreases or eliminates said functional activity.01-20-2011
20130059325ISOLATED POPULATIONS OF ADULT RENAL CELLS AND METHODS OF ISOLATING AND USING SAME - A method of generating a nephrospheroid is disclosed. The method comprises culturing human adult kidney cells in a culture medium under non-adherent conditions. Uses thereof and other renal cell populations are also disclosed.03-07-2013
20130059324SYSTEMS AND METHODS FOR MECHANICALLY STRAINED CELL CULTURE - A method for in vitro drug screening can include: providing one or more cells in vitro; introducing a chemical to the one or more cells; introducing a mechanical strain to the one or more cells in the presence of the chemical; and determining whether or not the chemical has bioactivity with respect to the one or more cells under the mechanical strain. The mechanical strain can be implemented with a well plate defining a plurality of cell culture wells and having one or more flexible substrates associated with the well plate so that each cell culture well has a fluid tight flexible cell culture substrate. A dynamic in vitro screening device can include: a top plate defining one or more cell culture wells; one or more flexible cell culture substrates operably coupled with the top plate to form fluid tight cell culture well bottoms; an a pin plate under the top plate with the one or more flexible cell culture substrates therebetween, the pin plate having one or more pin members associated with the one or more cell culture wells.03-07-2013
20130059323Detection of Overtraining Syndrome in an Individual - The onset of or the existence of overtraining syndrome in an individual is detected by a method which comprises a) contacting leucocytes in, or obtained from, a blood sample provided by the individual with a luminescence reagent which emits light on reaction with an oxidant; b) adding an activator to the mixture of leucocytes and the luminescence reagent; c) continuously monitoring and/or measuring light emitted by the luminescence reagent over a predetermined time period commencing before and ending after the addition of the activator; and d) assessing the light emission. The individual may be a human, for example an elite athlete, or a non-human mammal, for example a racehorse. A diagnostic kit, for carrying out the method, comprising a luminescence reagent which emits light on reaction with an oxidant, an activator and a library of standard signature light emission curves is also disclosed.03-07-2013
20100120082Optimization of Process Variables in Oxygen Enriched Fermentors Through Process Controls - Methods and systems are provided for controlling the addition of oxygen in fermentations to achieve a desired oxygen consumption and substrate yield in fermentation cell cultures. In one aspect, the invention provides a method for regulating the addition of oxygen (O05-13-2010
20090269797 STRAINS OF ZYMOMONAS MOBILIS FOR FERMENTATION OF BIOMASS - The present invention relates to methods of obtaining 10-29-2009
20090269796METHODS OF DETECTING AND TREATING MYOCARDIAL ISCHEMIA AND MYOCARDIAL INFARCTION - Methods of detecting and treating myocardial ischemia and myocardial infarction based on the differential expression of metabolic products are described herein.10-29-2009
20090269794SCREENING METHOD FOR COMPOUND INHIBITING DEVELOPMENT OR PROGRESSION OF FAMILIAL AMYOTROPHIC LATERAL SCLEROSIS AND DIAGNOSTIC METHOD FOR FAMILIAL AMYOTROPHIC LATERAL SCLEROSIS - A screening method comprising the following steps is provided. 10-29-2009
20100055734Methods for Diagnosis and Intervention of Hepatic Disorders - The disclosure provides a method for quantification of hepatic function in a subject comprising measuring the clearance of an orally administered isotopically labeled cholic acid in a subject with, or suspected of having or developing, a hepatic disorder, for example, chronic hepatitis C. The disclosure further provides methods and kits for assessment of hepatic function.03-04-2010
20130065267CONJUGATES AND METHODS FOR MEASURING CHAPERONE-MEDIATED AUTOPHAGY - This disclosure relates to methods of detecting chaperone-mediated autophagy. In some embodiments, the disclosure relates to methods of measuring chaperone-mediated autophagy in living cells and in purified lysosomes. In some embodiments, the disclosure relates to methods of detecting, diagnosing, monitoring, and/or treating lysosomal diseases in a subject.03-14-2013
20130065266Disease Diagnosis Method, Marker Screening Method and Marker Using TOF-SIMS - The present invention relates to a disease diagnosis method, a marker screening method, and a marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS), and more particularly, to a large intestine cancer diagnosis method, a large intestine cancer marker screening method, and a large intestine cancer marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS). Specifically, the present invention provides a method diagnosing a disease using a pattern of secondary ion mass (m/z) peaks of biological samples measured using a time-of-flight secondary ion mass spectrometry (TOF-SIMS) as a marker, a marker screening method being a reference judging an existence or non-existence of a disease, and a marker configured of specific secondary ion mass peaks.03-14-2013
20110020856PERIFUSION DEVICE - A perifusion device includes at least one sample container for cells, the sample container having an inlet and an outlet. The container receives test liquid through the inlet and discharges the liquid through the outlet. A manifold having a plurality of liquid inlets, control valves, and liquid outlets can be provided so that the flow of liquids from source containers to the sample containers can be varied and controlled. A receptacle housing has a plurality of receptacles for receiving fluid from the outlet of the sample container. A drive is connected to the receptacle housing for moving the receptacle housing such that liquid samples are collected sequentially from the outlet of the sample containers. A programmable controller can be provided to control movement of the receptacle housing at predetermined times, and to record data identifying liquid samples in the receptacles. The test liquid includes at least one stimuli for the cells, which can be the presence, absence, or concentration of a compound in the liquid, or a physical property of the liquid such as temperature. The liquid collected in the receptacles is analyzed to determine the response of the cells to the stimuli.01-27-2011
20090233325SCREENING METHOD OF NESFATIN-1-ACTION REGULATING SUBSTANCE OR NESFATIN-1-LIKE ACTION SUBSTANCE WITH THE USE OF RECEPTOR PROTEIN SELECTED FROM THE GROUP CONSISTING OF GPR3, GPR6 AND GPR12 - An object of the present invention is to identify a Nesfatin-1 receptor, and to provide a method for screening or designing a Nesfatin-1-action regulating substance or a Nesfatin-1-like action substance using the Nesfatin-1 receptor.09-17-2009
20090233324Methods for Diagnosing Cancer Using Samples Collected From A Central Vein Location or an Arterial Location - The invention encompasses methods for selectively enriching in rare particles from blood samples harvested from the vein jugular vein the femoral vein, the subclavian vein, or an artery. These rare particles can be circulating tumor cells, circulating stem cells, or fragments thereof. Blood samples harvested from different sources can contain higher or lower concentrations of rare particles. The rare particles can be enriched by applying the blood samples to a microfluidic device with a two dimensional array of obstacles.09-17-2009
20090233323METHOD FOR ANALYSIS OF NKT CELL FUNCTION - The present invention provides a superior method of functional analysis of NKT cells, which enables function analysis of low frequency NKT cells, is independent of the function of autologous APCs, and can avoid an influence of secondary factors and the like. More specifically, the present invention provides a method of functional analysis of human NKT cells including (a) cocultivating a mononuclear cell derived from human peripheral blood with a CD1d-expressing antigen presenting cell derived from a heterologous animal, and (b) evaluating the functionality of NKT cells with the number of NKT cells and/or a substance specific to functional NKT cells as an index; a reagent for analysis of human NKT cells containing a CD1d-expressing antigen presenting cell derived from a heterologous animal; a kit containing (a) a CD1d-expressing antigen presenting cell derived from a heterologous animal, and (b) at least one reagent selected from the group consisting of a reagent for selection of human mononuclear cell, a reagent for measurement of the number of human NKT cells and a reagent for measurement of a substance specific to human functional NKT cells; and the like.09-17-2009
20090047702Method for Identifying Modulators of Keah6 Useful for Treating Alzheimer's Disease - Methods for identifying modulators of KEAH6 are described. The methods are particularly useful for identifying analytes that antagonize KEAH6's effect on processing of amyloid precursor protein to Aβ peptide and thus useful for identifying analytes that can be used for treating Alzheimer disease.02-19-2009
20090047700Flourescence Assay for MTP Activity - The present invention is directed to methods for assaying microsomal triglyceride transfer protein (MTP) which are amenable to automation and high throughput screening. The assays may be used to measure MTP activity in cell and tissue homogenates as well as purified MTP. Also provided are methods of measuring levels of lipids transferred by MTP. The methods provided by the present invention have the advantages of ease, rapidity, sensitivity, avoidance of the use of radioactivity, versatility in studying different lipid transfer activities by purified and cellular MTP and the ability to measure inhibitory activity. In addition, methods of identifying compounds that modulate the lipid transfer activity of MTP are provided. Kits for measuring the lipid transfer activity of MTP as well as net transfer of lipid by MTP are provided by the present invention.02-19-2009
20120115180USE OF ANIMAL CELLS FOR SCREENING PROBIOTIC BACTERIA STRAINS - The present invention relates to an in vitro and/or ex vivo method of screening probiotic bacterial strains. The present invention also relates to a method of assessing quality of probiotic culture. In addition, the present invention relates to use of animal cells in screening of a probiotic strain. The present invention also relates to use of animal cells in assessing quality of probiotic culture.05-10-2012
20120115179ARRANGEMENT, SUBSTRATE AND METHOD FOR PREPARING A CELL SAMPLE - A flexible, thin, elongated band is used as a substrate. Similarly to a magnetic tape, the band is unwound from a feed reel and is transported past an outlet opening of a receptacle containing the cells such that the cells are poured onto the band. Subsequently, the band containing the cells applied thereon is wound onto a take-up reel. The take-up reel is fixed on a drive shaft which can be rotated by a drive mechanism. The rotation thus achieved has the effect that the band is unwound, transported past the outlet opening and finally wound up. In addition, spacers are provided at the upper surface of the band in order to prevent the contacting of radially adjacent sections of the band containing the cells in a wound-up state.05-10-2012
20090291467Near Infrared Fluorophore for the Selective Labelling of Membranes in Cells - The present invention relates to anthraquinone derivatives which act as a fluorophore, to a process for producing said anthraquinone derivatives and their use as fluorophores for staining membranes in live or fixed cells.11-26-2009
20090305323LIPIDOMICS APPROACHES FOR CENTRAL NERVOUS SYSTEM DISORDERS - The present invention has utilized the power of lipidomics to profile lipid metabolites and to characterize changes in lipid metabolism as they relate to CNS disorders. Lipidomic signatures can guide the development of diagnostic, prognostic and surrogate markers for CNS disorders; identification of new targets for drug design based on highlighted perturbed pathways; stratify patients with CNS disorders as to which pathways are impaired, and facilitate the determination of which patients with CNS disorders are candidates for a particular therapy, i.e. provide the tools for a personalized approach to therapy; identify which patients are responding or are developing side effects to a treatment; design of modified antipsychotics that have less metabolic side effects and enhanced activity; overcome the lag phase in response to some treatments; and find better combination therapies for CNS disorders that target the pathways that are impaired (e.g., impairments in lipid and/or carbohydrate metabolism).12-10-2009
20090305324CYTOTOXIC T-CELL EPITOPE PEPTIDES THAT SPECIFICALLY ATTACK EPSTEIN-BARR VIRUS-INFECTED CELLS AND USES THEREOF - The present inventors introduced mRNAs for the Epstein-Barr virus proteins LMP1 and EBNA1 into antigen-presenting cells, and as a result, demonstrated that the cells induced Epstein-Barr virus-specific cytotoxic T cells. The present inventors also demonstrated that the cytotoxic T cells recognized epitope peptides presented via HLA-A*0206, HLA-Cw*0303, or HLA-Cw*0304, inhibited the outgrowth of Epstein-Barr virus-infected B cells, and lysed Epstein-Barr virus-infected NK lymphomas and NK cells.12-10-2009
20090317852Engineered Cell Growth on Polymeric Films and Biotechnological Applications Thereof - A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.12-24-2009
20090029406Npy Y4 Agonist as Therapeutic Agent for Disease Accompanied by Intestinal Tract Dysfunction - The present invention has its object to provide an agent capable of ameliorating the intestinal tract dysfunction through a novel mechanism of action. It also has its object to provide a method for the evaluation of a compound, including the screening of the agent. A disease accompanied by intestinal tract dysfunction can be ameliorated or treated by using an NPY Y4 agonist as a therapeutic agent. A compound capable of ameliorating or treating a disease accompanied by intestinal tract dysfunction can be evaluated or screened by evaluating a compound targeting to an NPY Y4 receptor.01-29-2009
20090017484MARKERS FOR ATHEROSCLEROSIS - A method for the diagnosis, prognosis or identification of a predisposition towards atherosclerosis and the susceptibility to the development of atherosclerotic plaques and/or vascular obstruction is described, the method comprising analysing a sample to determine the percentage frequency of multiple sub-populations of T-lymphocytes and comparing the data obtained against comparative and/or control data.01-15-2009
20080299601Adhering Surfaces - The present invention relates, in part, to compositions useful for cell culture having one surface in adherence to another surface, e.g., a cell culture matrix in adherence to a surface. The present invention also relates, in part, to methods of adhering one surface to another surface, e.g., adhering a cell culture matrix to a surface, and compositions relating to such methods. The present invention also provides in part, methods for adhering a cell to a surface. Related methods are also provided for determining the effect of at least one compound on a cell(s).12-04-2008
20080199899Method For Screening Modulators of Mitochondrial Functioning and New Modulators Obtained - The invention relates to a method for screening modulators of mitochondrial function comprising adding a compound to be tested in a purified, isolated mitochondria preparation and simultaneously using fluorimetric analysis of mitochondrial morphology, and especially real-time fluorimetric analysis, combining analysis of morphometric parameters (SSC/FSC parameters) with analysis of membrane integrity by dye fluorescence. Application to the obtention of peptides which induce mitochondrial membrane permeabilization.08-21-2008
20090011456Methods for the Diagnosis and Prognosis of Graft Versus Host Disease By Measurement of Peripheral Cd3+Cd4+Cd8Beta+ Cells - Acute graft versus host disease (GvHD) is one of the most significant clinical problems in allogeneic blood and marrow transplantation. Currently, there is no unequivocal diagnostic test for GvHD until the disease is well developed and can be recognized histologically. The invention provides a blood based test for diagnosis and/or prognosis of GvHD, allowing assessment of risk for developing GvHD prior to appearance of clinical symptoms. Using flow cytometry, peripheral blood mononuclear cells are assessed for an increase in proportion and fluctuation of CD301-08-2009
20090011455DISEASE MODEL INCORPORATION INTO AN ARTIFICIAL IMMUNE SYSTEM (AIS) - The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the tissues of the immune system in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates and other materials in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system, coupled with disease models to provide a more complete representation of an immune response.01-08-2009
20090011453Methods for Producing Proteins Using Hamster Igf-1 - A polypeptide selected from the group consisting of: (1) a polypeptide having the amino acid sequence of SEQ ID NO: 1, and (2) a polypeptide having an amino acid sequence in which the third amino acid of the amino acid sequence of SEQ ID NO: 1 is substituted by another amino acid and having insulin-like growth factor 1 (IGF-1) activity; a DNA encoding said polypeptide; a vector containing said DNA; a host cell containing said vector; and a method for preparing a recombinant protein using said polypeptide.01-08-2009
20090011452Method of Prognosis of Mental Diseases, E.G. Autism and Cerebral Palsy - The invention provides a method of prognosis for applied behavioral analysis treatment of mental disease, in particular autism or cerebral palsy, in a human subject. The method includes analyzing a sample of body tissue or fluid from the subject for the presence or absence of a chemical marker indicative of the likelihood of success or failure of applied behavioral analysis treatment of the mental disease, and optionally, based on the analysis, beginning, continuing or ceasing applied behavioral analysis treatment of the subject. The sample is preferably an urine sample, and the preferred prognostic markers are: gluten derivatives, indolyl-3-acryloylglycine (IAG), serotonin uptake stimulator, β-casomorphineamides, gliadinomorphine, β-casomorphines, deltorphins, Demorphine, glutemorphine, gluten exophins, compounds of molecular weight of 687 Daltons.01-08-2009
20090011451MICROFLUIDIC DEVICE AND LEUCOCYTE ANTIGEN MEDIATED MICROFLUIDIC ASSAY - The present invention relates to an leucocyte antigen mediated microfluidic assay and a microfluidic device for analyzing a subjects' body fluids containing leucocytes to determine if the subject has been previously exposed to a predetermined antigen.01-08-2009
20100086960METHODS FOR THE DIAGNOSIS OF OVARIAN CANCER HEALTH STATES AND RISK OF OVARIAN CANCER HEALTH STATES - The present invention describes a method for predicting a health-state indicative of the presence of ovarian cancer (OC). The method measures the intensities of specific small organic molecules, called metabolites, in a blood sample from a patient with an undetermined health-state, and compares these intensities to those observed in a population of healthy individuals and/or to the intensities previously observed in a population of confirmed ovarian cancer-positive individuals. Specifically, the present invention relates to the diagnosis of OC through the measurement of vitamin E isoforms and related metabolites. The method enables a practitioner to determine the probability that a screened patient is positive or at risk for ovarian cancer.04-08-2010
20100086961Small Volume In Vitro Analyte Sensor and Methods of Making - A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.04-08-2010
20090017485Alzheimer's Disease Examination Method - [Problems] To enable convenient and accurate examination of Alzheimer's disease.01-15-2009
20130164773DISPOSABLE CARTRIDGE FOR FLUID ANALYSIS - A disposable blood analysis cartridge for analyzing a blood sample including an optical light scattering measurement channel is described. In use, processed sample may be introduced into a sheath fluid channel at an angle, α, of approximately 90 degrees, relative to the direction of flow of the sheath fluid. In addition, delivering the sample from the side into the sheath fluid may facilitate better positioning of the core within the hydrodynamic focusing channel for measurement.06-27-2013
20080311613ARTIFICIAL SKIN SURFACE FILM LIQUIDS - An artificial skin surface film liquid (SSFL) is disclosed that mimics the chemical composition and biological action of both human sweat and sebum. The artificial sweat formulation contains a comprehensive combination of constituents. When combined with the sebum component it is particularly effective at modeling the effect of the skin surface film liquid on agents in contact with the skin. Also provided are methods of making and using the disclosed artificial skin surface compositions.12-18-2008
20080311608Antagonist of the oncogenic activity of the protein MDM2, and use thereof in the treatment of cancers - The present invention relates to the utilization of a compound capable of antagonizing at least partially the oncogenic activity of the protein Mdm2 for the preparation of a pharmaceutical composition intended more particularly to a treatment of cancers with no p53 context. It further relates to the viral vector comprising a nucleic acid sequence coding for a compound capable of inhibiting at least partially the oncogenic activity of the protein Mdm2, and to a corresponding pharmaceutical composition.12-18-2008
20120237966Light Controlled Protein Dimerization in Cells - Compositions and methods for light controlled protein-protein interactions in a living cell. Two interacting PICL (protein interaction controlled by light) polypeptides are provided. The first polypeptide comprises an LOV (Light, Oxygen or Voltage) domain, which domain is a light sensor that uses flavin mononucleotide (FMN) as a chromophore. The second polypeptide, specifically interacts with the L polypeptide upon light activation of the LOV domain. One or both of the polypeptides are fused to a cellular protein of interest. Upon exposure to light, a targeted interaction between cellular proteins occurs. The ability to regulate protein-protein interactions with subcellular resolution using light is useful for controlling biochemical processes such transcription, receptor activation, protein degradation, synapse formation, etc. in cells and animals.09-20-2012
20120237965SYSTEMS AND METHODS FOR A CONTINUOUS CULTURE BIOSENSOR - A continuous culture system of genetically modified yeast or another biological organism detects contaminants in water through production of a fluorescent chemical continuously produced by the organism after it is contacted with a threshold concentration of a contaminant. Alternatively, a biological organism can detect toxins or extra nutrients by detecting a change in growth rate. Biological organisms can also be measured for mutagenic changes by comparing their genome with a control sample. A network of continuous culture systems may be used as part of a water contamination detection system for real-time water monitoring of contaminants from multiple sources simultaneously.09-20-2012
20120237964LUMINOGEN COMPOUNDS AND THE USE OF THE SAME FOR BIOSENSING AND CELLULAR IMAGING - Provided herein are a luminogen compound of formula (I) including a AIE luminophore moiety conjugated with a maleimide moiety and a use of the same for detecting thiol groups in biomolecules. Also provided is a dye molecule, a biosensor or a bioprobe comprising the luminogen compound of formula (I) in use for detecting thiol groups in biomolecules. The detection method of the present subject matter not only has high thio-selectivity and sensitivity, but also is rapid, convenient and handy.09-20-2012
20120237963APPARATUS AND METHOD FOR ASSESSING COMPOSTABILITY OR BIODEGRADABILITY - An apparatus and method for performing compostability tests that provides significantly more data and reveals trends much more quickly than previously described apparatus. In a first aspect, the invention provides an apparatus, comprising: a flowmeter for metering a flow of oxygen-containing gas to a humidifier; a bioreactor comprising a body, a gas inlet mounted on the body and connected to the humidifier, a dispersive element for distributing the flow of humidified gas throughout the body, and a gas outlet; a first moisture trap connected to the outlet a mass flow meter connected to the first moisture trap for accurately measuring the mass flow from the outlet; and a non-dispersive IR detector for measuring the carbon dioxide in the gas from the outlet. Convenient embodiments include a digitally controlled electronic manifold for sequentially directing gas flows from multiple reactors to the analyzer/detector.09-20-2012
20130164772METHODS FOR THE EXTRACTION AND IDENTIFICATION OF ANTIPERSPIRANT SALT PLUGS - Methods for the extraction and identification of antiperspirant salt plugs are provided. In accordance with an embodiment, a method for the extraction and identification of antiperspirant salt plugs comprises applying to skin an antiperspirant product and applying to the skin a transparent slide having a glue disposed thereon, wherein the glue is placed in contact with an area of the skin having the antiperspirant product applied thereto. The glue is allowed to cure to the area of the skin. The transparent slide and a sample coupled to the transparent slide are removed. The sample comprises the cured glue and first skin layers from the area of the skin. A stain sensitive to aluminum is applied to the sample. The sample is differentiated and the sample is allowed to dry.06-27-2013
20130164774METHODS, COMPOSITIONS AND KITS FOR ASSAYING MITOCHONDRIAL FUNCTION - The invention provides methods, compositions, devices, and kits relating to the use of cholesterol-dependent cytolysins (e.g., PFOs) for measuring intracellular mitochondrial activity.06-27-2013
20130164775MONITORING CELL AND METHOD OF ANALYZING CELL AND TISSUE GROWTH - The invention relates to a monitoring cell for analyzing a cell and tissue growth, in which a carrier object (06-27-2013
20120270259FISH CANCER MODEL - The present invention is directed to fish whose genome has integrated therein an oncogenic nucleic acid operably linked to a promoter. Methods of making the fish and methods for their use are also provided. The fish may advantageously be utilized in methods of screening for drugs or agents that modulate oncogene-mediated neoplastic or hyperplasic transformation, or that modulate sensitivity to chemotherapy or radiation therapy Immortal tumor cells lines, methods of making immortal tumor cells lines and methods of their use are also provided.10-25-2012
20100261223DEVICE FOR DETECTING COMPONENTS IN A FLUID - A device and a method for detecting components in blood, in particular for determining the concentration of components in blood or water is provided. Moreover, a use of a device and/or a method for determining components in blood is provided and a kit including the device and a fluorescence standard.10-14-2010
20100196945DEVICE AND METHOD FOR DETERMINING THE ERYTHROCYTE SEDIMENTATION RATE IN A BLOOD SAMPLE - The invention discloses a blood analyzing device (08-05-2010
20110177545G-PROTEINS07-21-2011
20100297685METHODS FOR CULTURING AND ANALYZING CELLS - The present invention generally relates to methods for culturing and analyzing cells using liquid bridges.11-25-2010
20090291468Method and Device for Monitoring Medication Usage - The present invention provides methods for detecting and quantifying metabolites in a biological sample by measuring the concentration of a test metabolite in the sample and comparing that concentration against the concentration of the reference metabolite; enabling accurate metabolite concentration measurements to determine aberrant drug usage patterns. Also disclosed is an analytical testing device and related computer-assisted products for detecting and quantifying metabolites in a biological sample efficiently and accurately.11-26-2009
20120064562Subterranean Alternating Digester System and Method - An alternating anaerobic and aerobic digestion system and method of forming same includes a subterranean enclosure configured to hold organic matter. The enclosure has a plurality of conduits in a bottom surface of the enclosure. The digestion system further includes an irrigation system configured to dispense a liquid from a top portion of the enclosure and to recover a percolated liquid from a bottom portion of the enclosure, a ventilation system configured to provide air flow to the bottom portion of the enclosure, and a gas-tight membrane cover configured to cover the enclosure.03-15-2012
20120009619UNITARY CARTRIDGE FOR PARTICLE PROCESSING - A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.01-12-2012
20120270258METHODS AND KITS FOR DETECTING HEMOGLOBIN IN TEST SAMPLES - The present invention relates to methods of detecting hemoglobin in a test sample. These methods can be used to diagnose a subject suffering from a genetic disorder relating to hemoglobin metabolism, to determine the eligibility of a subject to be a blood donor, to determine the age of a stored blood sample or to identify a hemolyzed plasma sample. The present invention also relates to kits for use in the above described methods.10-25-2012
20120135449ITERATIVE STAINING OF BIOLOGICAL SAMPLES - Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.05-31-2012
20120077218SICKLE CONFIRM MODIFIED HEMOGLOBIN SOLUBILITY TEST - The present invention provides a method for determining sickle-cell zygosity in a subject, comprising: forming a first solution comprising a sample from the subject, a phosphate buffer, a detergent, and a reducing agent; subjecting the first solution to centrifugation to form a second solution and a supernatant; taking a color reading of the supernatant and of the second solution; optionally filtering the second solution to form a filtrate and a precipitate, and optionally measuring the amount of the precipitation and the absorbance of the filtrate or taking a color reading of the filtrate.03-29-2012
20120183988INTEIN-BASED FLUORESCENT BIO-CIRCUIT FOR VITAMIN D DETECTION - This invention is directed to an intein-based vitamin D fluorescent biosensor that accurately and rapidly measures 1α,25-hydroxyvitamin D3. Measurement at picomolar quantities is noted. Particular reference is made to the use of 07-19-2012
20110229924Fluorescent ion indicators and their applications - Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.09-22-2011
20120276571METHOD OF ANALYZING FORMATION AND PHASE TRANSITION CHARACTERISTIC OF AMORPHOUS CALCIUM CARBONATE - Provided is a method of analyzing a formation and a phase transition characteristic of amorphous calcium carbonate that may adjust a preferred orientation in crystalline calcium carbonate as well as an amorphous state of calcium carbonate using a water-soluble material containing an amino acid in an operation of forming calcium carbonate. It is possible to handle issues of a limit of a sampling and a standard pattern of an analysis scheme in in vitro calcium carbonate crystallization test by adjusting a holding time of amorphous calcium carbonate or a preferred orientation of a crystal calcium carbonate when forming calcium carbonate using a water-soluble material containing an amino acid. Further, it is possible to verify further characteristics of elements that adjust a formation of a biological material, which may be used for a synthesis of a new material in tissue engineering as well as for an biomineralizaton process.11-01-2012
20100062477METHODS OF ISOLATING AND PROPAGATING STEM CELLS FROM BENIGN TUMORS - The present invention describes benign tumor stem cells, a method of isolating the benign tumor stem cells, a method of generating the benign tumor stem cells and a method of using the benign tumor stem cells. Uses of the benign tumor stem cells, such as pituitary stem cells include but are not limited to producing pituitary hormones and identifying drugs to treat pituitary disease conditions or pituitary-related disease conditions.03-11-2010
20100035293Pulsing of Bile Compartments in Sandwich-Cultured Hepatocytes - A method of pulsing cultured hepatocytes, such as sandwich-cultured hepatocytes. The method includes providing a culture of hepatocytes, the culture having at least one bile canaliculus; exposing the culture of hepatocytes to a calcium-free buffer, whereby the contents of the at least one bile canaliculus are released; and removing the calcium-free buffer Pulsing cultured hepatocytes can reduce cholestasis arid can provide an in vitro culture of hepatocytes the more closely reflects in vivo hepatocyte characteristics.02-11-2010
20090181419Method for Quantitatively Determining the Apolipoprotein AI Content in the HDL 2B Subfraction of HDL Cholesterol Subfractions - The invention provides a method (e.g., a computer algorithm) for calculating a number of particles in a HDL subfraction. The method features the steps of: 1) measuring an initial distribution of HDL particles (e.g., a relative mass distribution) from a blood sample; 2) processing the initial distribution of HDL particles with a mathematical model to determine a modified distribution of HDL particles (e.g., a relative particle distribution); 3) determining a total apo-AI content value from a blood sample; and 4) analyzing both the modified distribution of particles and the total apo-AI content value to calculate the apo-AI content value in an HDL subfraction.07-16-2009
20120190056Encoded Microparticles - The present invention relates to an encoded microparticle for labeling an isolated cell or an isolated embryo characterized in that it is made of a biocompatible material and its external shape comprises a code by which it can be identified. The use of an encoded microparticle for labeling and/or tracking isolated biological material. A method of tracking an encoded microparticle in or attached to an isolated cell or embryo using an optical microscope, preferably an inverted optical microscope with an objective substantially between 20×-100×.07-26-2012
20090098595Tumor Marker For Ovarian Cancer Diagnosis - The present invention relates to a tumor marker for diagnosis of ovarian cancer, which is selected from the group consisting of galectin-1, cathepsin B, MHC class I antigen, heat shock protein (HSP) 27, ubiquitin carboxy-termal esterase L1, plasma retinol-binding protein (PRBP), transthyretin, SH3 binding glutamate-rich protein, tubulin-specific chaperone A, RNA binding protein regulatory subunit, γ-actin, tropomyosin and calcium/calmodulin-stimulated cyclic nucleotide phosphatase. The ovarian cancer is diagnosed effectively and efficiently based on detecting the expression levels of the tumor markers in the invention from the ovarian tissue sample of an individual to be diagnosed.04-16-2009
20090098594Methods for diagnosis prognosis and methods of treatment - This invention is directed to methods and compositions for diagnosis, prognosis and for determining methods of treatment. The physiological status of cells present in a sample (e.g. clinical sample) can be used in diagnosis or prognosis of a condition (e.g. Chronic Lymphocytic Leukemia), in patient selection for therapy, to monitor treatment and to modify or optimize therapeutic regimens. The physiological status of a cell can be determined by comparing the intracellular status of one or more activation elements (e.g. the phosphorylation status of a signaling molecule) in a cell (e.g. a cancer cell) to that of another cell (e.g. a normal cell). The physiological status of a cell can be further classified by adding one or more modulators (e.g. an inhibitor or activator) to the cell in question. In some embodiments, the invention is directed to methods of determining a phenotypic profile of a population of cells.04-16-2009
20110281295METHOD AND DEVICE FOR CULTURING ALGAE - According to one aspect, the invention relates to a device for culturing algae with natural light, including an enclosure with a culturing medium and the algae to be cultured, and a substrate arranged to receive solar radiation in order to perform photoconversion of said solar radiation, the substrate including at least one luminescent compound making it possible to reemit radiation having a spectrum adapted to the optimisation of a biological parameter of interest resulting from the photosynthesis of said algae.11-17-2011
20090311735METHOD FOR STEM CELL CULTURE AND CELLS DERIVED THEREFROM - There is described a method of promoting the attachment, survival and/or proliferation of a stem cell in culture, the method comprising culturing a stem cell on a positively-charged support surface. There are also provided a cell composition prepared according to the method of the invention.12-17-2009
20110281292Compositions and methods for identifying antigens which elicit an immune response - This invention relates to an expression vector wherein said expression vector comprises a polynucleotide promoter sequence, a polynucleotide encoding a signal sequence, a polynucleotide encoding an antigen protein or peptide, a polynucleotide encoding a cell binding element, and a polynucleotide polyadenylation sequence all operatively linked. More particularly, it relates to the method of eliciting an immune response directed against an antigen in a mammal comprising the steps of introducing the expression vector into a cell, expressing the vector to produce an antigen under conditions wherein the antigen is secreted from the cell, endocytosing the secreted antigen into the cell, processing the antigen, and presenting fragments to a receptor to elicit a T-cell response. In addition, this invention relates to a vaccine and a method of use. The invention also relates to the method of identifying MHC-II restricted epitopes.11-17-2011
20110281294METHOD AND DEVICE FOR MEASURING ADHESION FORCES - A measurement head for measuring the adhesion force between a surface and a particle attached to the surface, a force measurement system and a method for measuring the adhesion force between a surface and a particle attached to the surface are described.11-17-2011
20110281293PREPARATION AND ITS USE OF DERIVATIZATION REAGENT FOR DETECTING L-CARNITINE OR D-CARNITINE - A preparation method and its use of derivatisation reagent for detecting L-carnitine or D-carnitine are provided. The present reagent is stable. It can be used for detecting L-carnitine or D-carnitine accurately and sensitively. That is to say, the reagent is applied to detecting the amount of synthesized or natural L-carnitine and the amount of mixing D-carnitine. The compound reagent is used for determining the chiral isomers of chemicals, biological reagents, health care reagents, cosmetic, body fluids and various foods, which contain L-carnitine or/and D-carnitine, and optical isomers of other chiral amino acids.11-17-2011
20110281291Identification and/or characterization of a microbial agent using taxonomic hierarchical classification - A method for identification and/or characterization of a microbial agent present in a sample includes a step of analytical test data (e.g., obtaining intrinsic fluorescence values over a range of emission wavelengths) from the microbial agent. The analytical test data is transformed thereby minimizing strain to strain variations within an organism group. With the aid of a programmed computer, a multi-level classification algorithm coded as a set of processing instructions operates on the transformed analytic test data. The multiple levels correspond to different levels in a taxonomic hierarchy for microbial agents suspected of being in the sample.11-17-2011
20100248287DECONTAMINATION OF PRION-CONTAMINATED SURFACES WITH PHENOLS - A method of decontaminating a surface or liquid which is contaminated with prions includes treating the surface with a composition which includes one or more phenol. Phenols which are particularly effective include p-chloro-m-xylanol, thymol, triclosan, 4-chloro, 3-methylphenol, pentachlorophenol, hexachlorophene, 2,2-methyl-bis(4-chlorophenol), and p-phenylphenol.09-30-2010
20100261224DISCOVERY AND VALIDATION OF CANCER BIOMARKERS USING A PROTEIN ANALYSIS METHODOLOGY TO ANALYZE SPECIMENS - Methods are provided for the analysis, including the serial analysis, of very small samples of tissue. The methods utilize a nanofluidic proteomic immunoassay (NIA) to quantify total and low-abundance protein isoforms in a small amount of lysate. NIA detection accurately measure oncoprotein expression and activation in limited clinical specimens, including isoforms that differ in post-translational modifications, such as phosphorylation, and the like. The NIA detection method combines isoelectric protein focusing and antibody detection in a nanofluidic system.10-14-2010
20100003714System and Method for Determining Fill Volume in a Container - A system and method for detecting a pathogen in a sample is provided, the system capable of measuring the volume of a sample in a container through the use of various measurement technologies, thereby ensuring that a user is aware of volumes not meeting specification and/or allowing correction of results to account for the out-of-specification sample.01-07-2010
20090148883BIOMARKER FOR ASSESSING RESPONSE TO FMS TREATMENT - A biomarker that correlates to treatment with drugs that inhibit FMS is disclosed. This biomarker has been shown to have utility in assessing response to the compounds. The plasma level of the biomarker is increased upon treatment with FMS inhibitor compounds, thus indicating that this biomarker is involved in FMS activity.06-11-2009
20090136990METHODS FOR IDENTIFYING SUBSTANCES FOR THE TREATMENT OF ALZHEIMER'S DISEASE - The present invention relates to a method for diagnosing Alzheimer's disease and Parkinson's disease in a subject by analyzing the expression of Semaphorin 3 and downstream effectors. It also provides a method for identifying a substance useful in the prevention or treatment of Alzheimer's disease and Parkinson's disease, and a method of using such substance in the treatment of Alzheimer's disease and Parkinson's disease.05-28-2009
20080286825Methods for Diagnosing and Monitoring the Progression of Cancer - Methods for measuring c-Met levels in urine and blood samples are provided. Methods for diagnosis and prognosis evaluation for cancer are also provided.11-20-2008
20100297687DETECTION AND MEASUREMENT OF MASS CHANGE USING AN ELECTROMECHANICAL RESONATOR - A change in impedance of a electromechanical resonating sensor is utilized to detect and/or measure a change in mass accumulated on the sensor. The impedance is monitored at a fixed frequency. The fixed frequency may be at or near the resonance frequency of the sensor. In various configurations, the sensor comprises a quartz crystal microbalance sensor or a piezoelectric cantilever sensor.11-25-2010
20090286272Biomarkers for niemann-pick C disease and related disorders - Methods for screening or diagnosing subjects for disorders involving accumulation of one or more oxysterols such as cytotoxic oxysterol accumulation, Niemann-Pick C(NPC) disease, lysosomal storage diseases, cholesterol trafficking diseases, and neurodegenerative diseases. Also provided are methods for methods for screening or diagnosing subjects (including infants and neonatal subjects) for NPC disease, methods for monitoring the progression, remission, and clinical status of NPC disease, and methods for evaluating the efficacy of therapeutic treatment of NPC disease.11-19-2009
20100297689PLACENTAL STEM CELL POPULATIONS - The present invention provides placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. The invention also provides methods of differentiating the placental stem cells. The invention further provides methods of using the placental stem cells in assays and for transplanting.11-25-2010
20090233326METHOD FOR INDUCTION OF THE DIFFERENTIATION OF VISCERAL FAT CELL - A culture medium is disclosed for inducing the differentiation of visceral preadipocytes into mature visceral adipocytes; the culture medium contains 0.85 to 100 ng/mL insulin and 50 to 250 ng/mL IGF-1. Also disclosed is a method for using the culture medium to induce the differentiation of visceral preadipocytes into mature visceral adipocytes. Use of the differentiation induction system of the present invention enables a substantial induction of adipocyte differentiation without the addition of synthetic differentiation inducers or high insulin concentrations. The mature adipocytes obtained by the differentiation induction system of the present invention are useful for research into the biochemistry and physiology of adipocytes, for screening drugs effective for the treatment of lifestyle-related diseases such as obesity and type 2 diabetes, and for developing diagnostic reagents.09-17-2009
20090104645Vectors And Methods For Identifying Proteins Amenable To Crystallization - The present invention provides for methods, systems and materials that may be used to identify and select proteins that are amenable to crystallization. While previous methods of screening proteins for crystallization required at least microgram quantities of purified protein, the inventive method can be practiced using nanogram quantities of unpurified protein.04-23-2009
20100143962NANOPARTICULATE POLYCOSANOL FORMULATIONS & NOVEL POLYCOSANOL COMBINATIONS - The invention provides a culture device comprising a plurality of culture units, wherein each unit comprises a culture chamber, an inlet port for liquid supply of the culture and an outlet port for discharging liquid from the unit, wherein the inlet port is in fluid communication with the culture chamber and the culture chamber is in fluid communication with the outlet port for allowing a liquid flow through the culture chamber. The culture device is particularly suitable for testing immune cells and immunofunction in vitro. Aspects of the invention include a culture device and associated methods for cultivating immune cells and an in vitro method of analysing the effect of a test compound on immune cells.06-10-2010
20100015655Methods and Kits for Detecting and Quantifying Damage Caused by a Parasite - The present disclosure relates to assays and kits for detecting and quantifying damage caused by a parasite in a subject and monitoring the progression of parasitic disease in a subject.01-21-2010
20130023006ANALYZER, METHOD FOR MEASURING A SAMPLE, AND NON-TRANSITORY MACHINE-READABLE STORAGE MEDIUM - An analyzer for measuring a sample includes a display, measurement hardware configured to perform a quality control measurement on a vial containing a quality control (QC) sample, and a controller. The controller is in communication with the display and the measurement hardware and is configured to communicate, via the display, instructions to implement a QC measurement on a first vial containing a first QC sample. If a result of the QC measurement is within a pre-determined range the first vial passes the QC measurement. If the result of the QC measurement is not within the pre-determined range the first vial fails the QC measurement. If the first vial fails the QC measurement and if a number of times the first vial fails the QC measurement is less than a predetermined number, the controller is configured to communicate, via the display, instructions to repeat the QC measurement on the first vial.01-24-2013
20110124027PROBE ARRANGEMENT FOR EXCHANGING IN A CONTROLLABLE WAY LIQUIDS WITH MICRO-SIZED SAMPLES OF MATERIAL LIKE BIOLOGICAL CELLS - The invention relates to a probe arrangement (05-26-2011
20100062471Biomarkers for Multiple Sclerosis and Methods of Use Thereof - Biomarkers useful for identifying treatments for and monitoring treatment of patients with multiple sclerosis (MS) are provided, as well as methods for their identification, methods of diagnosing MS, relapse of MS patients and disease progression in MS patients.03-11-2010
20090305333Promoting Axon Regeneration in the Adult CNS through Control of Protein Translation - Survival of, or axon regeneration in a lesioned mature central nervous system (CNS) neuron is promoted by (a) contacting the neuron with a therapeutically effective amount of an exogenous activator of protein translation; and (b) detecting the resultant promotion of the survival of, or axon regeneration in the neuron.12-10-2009
20090029405LARGE STOKE SHIFT NIR DYES - A compound of the following formula:01-29-2009
20100330602Use of Soluble Galectin-3 (Gal-3) for Cancer Treatment - The present invention provides a method for preventing or treating cancer or tumorgenesis disorder comprising administering a prevention or treatment effective amount of a p53 mediated secretome component, such as Gal-3, to a patient in need thereof, thereby preventing or treating cancer or tumorgenesis disorders. Compositions and methods useful for modulating the secretome, including Gal-3, of a cell, comprising increasing extracellular levels of Gal-3, p53 expression, or expression of a downstream effector of p53, in the cell are also provided. Furthermore, methods for identifying tumor targets, diagnostic or prognostic indicators, and therapeutic strategies comprising determining extracellular levels of secreted proteins or secretomes, including Gal-3 are also provided. The present invention provides a novel tumor suppressive mechanism of p53 involving paracrine induction of apoptosis through extracellular Gal-3 levels. The invention also provides evidence that cancer cells are more susceptible to the treatment than normal cells, suggesting augmented expression of the receptor component to Gal3.12-30-2010
20090305327Mass Spectrometric Determination Of Blood Enzyme Activity - The invention relates to the determination of the nature and strength of enzymatic activity in blood using mass spectrometric measurement of a profile of the reaction products. The determination of the enzymatic activity can be used for medical diagnostics, for example, and also to check the effectiveness of medication. The invention provides a method whereby adding probe substances usually not present in blood offers standardized substrates for measuring the enzymatic activity. The probe substances may be added to whole blood, plasma, or serum. The mass spectrometric measurement of the reaction products, after their reversible immobilization on actively binding surfaces of solids, for example, can deliver biomarker patterns of the reaction products which may be indicators for metabolic anomalies or diseases, since these are often accompanied by the formation or activation of characteristic enzymes.12-10-2009
20100136597METHOD FOR MODULARTING ACTIVITY OF T LYMPHOCYTES - The invention relates to methods and compositions which modulate T lymphocyte activity. It has been found that two, T lymphocyte receptors, especially TCR and CD8, are present at a distance from each other on T lymphocyte surfaces. Via use of modulators which change the distance between these receptors, the activity of the T lymphocyte is modulated.06-03-2010
20110300574CELL-COUNTING METHOD FOR BLOOD HEMOLYSIS ANALYSIS IN FRAGILITY MEASUREMENT - A method of using cell counting to determine how much hemolysis occurs when a sample of red blood cells is subjected to stress, for purposes of either assessing stored blood quality and/or obtaining red blood cell fragility profiles. Cell-counting techniques and technologies can be employed to facilitate and enhance fragility measurements of red blood cells. There are many ways such “counting” can be achieved, including by visually counting each cell appearing under a microscope and projecting an estimated concentration based thereupon, or by utilizing a range of automatable technologies of various means. Using sophisticated image analysis algorithms, some such technologies also provide useful information about cell features or properties such as average size, distribution of sizes or volumes, and viability. Using cell counting to analyze post-stress hemolysis also permits conducting fragility assays without requiring centrifugation or other separation.12-08-2011
20110300573METHOD TO CHARACTERIZE BLOOD AND RED BLOOD CELLS VIA ERYTHROCYTE MEMBRANE FRAGILITY QUANTIFICATION - A method for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. The method comprises: a hemolysis step; an optical analysis step; and a computation step. The method is amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions. A vital aspect of this testing method is the accumulation of copious output and other associated data and the mathematical analyses thereof to optimize algorithms by which to characterize each subsequent test output as meaningfully as possible. While the present invention is directed toward applications in blood quality control, the core technology of “quantifying RBC fragility via stress-induced hemolysis and subsequent optical and computational analysis” could have broader application, such as in disease diagnosis.12-08-2011
20110300572IN VITRO METHOD AND APPARATUS FOR DETERMINING EFFICACY AND ACTION MECHANISMS OF A TOPICAL COMPOSITION ON VARIOUS SKIN COLOR TYPES - The present invention relates to in vitro methods for determining efficacy of topical compositions on various skin color types. These methods involve providing an artificial skin apparatus configured to approximate the color and diffuse reflectance characteristics of a predetermined human skin color type. The artificial skin apparatus includes an artificial skin substrate combined with a color background, with the color background correlating to the human skin color type. A topical composition of interest is applied to the artificial skin substrate of the artificial skin apparatus and then pre-irradiated. The pre-irradiated topical composition is then analyzed using relevant experimental techniques or assays for at least one efficacy parameter, including, for example, anti-ageing, photoprotective, sun protective, UVA/UVB protective, UVAI protective, photo stabilizing, and photosensitizing activities and action mechanisms of the topical composition on various skin color types. Also provided is an artificial skin apparatus and kit for use with the in vitro method.12-08-2011
20110300570METHOD AND SYSTEM FOR GENERATING SPATIALLY AND TEMPORALLY CONTROLLABLE CONCENTRATION GRADIENTS - The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. The present invention is directed to methods and systems for generating spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device. The formation of the concentration gradients can be initiated by an induced forward flow and further optimized during an induced backward flow. The forward and backward flows can be either passively induced and/or actively pumped. The centimeter-length gradients along the microfluidic channel can be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. In one example, a stabilized concentration gradient of a cardiac toxin, Alpha-cypermethrin, generated according to the invention was used to test the response of HL-1 cardiac cells in the microfluidic device, which correlated with toxicity data obtained from multi-well plates. The invention can be useful for bio-logical and chemical processes that require rapid generation of concentration gradients in a portable microfluidic device.12-08-2011
20110300569DATA ANALYSIS OF IMPEDANCE-BASED CARDIOMYOCYTE-BEATING SIGNALS AS DETECTED ON REAL-TIME CELL ANALYSIS (RTCA) CARDIO INSTRUMENTS - A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.12-08-2011
20110300568SYSTEMS AND METHODS FOR PROCESSING ALGAE CULTIVATION FLUID - Systems and methods for reducing an amount of unwanted living organisms within an algae cultivation fluid are provided herein. According to some embodiments, methods may include subjecting the algae cultivation fluid to an amount of cavitation, the amount of cavitation being defined by a pressure differential between a downstream pressure and a vapor pressure, the pressure differential divided by half of a product of a fluid density multiplied by a square of a velocity of an apparatus throat.12-08-2011
20120107862FACTORS - A method for determining a prognosis for benefit for a cancer patient receiving immunotherapy treatment involving (a) measuring a level of haematocrit and haemoglobin in a sample from the cancer patient, and (b) comparing the level of haematocrit in the sample to a reference level of platelets and comparing the level of haemoglobin in the sample to a reference level of haemoglobin, wherein a lower level of haematocrit and higher level of haemoglobin in the sample correlates with increased benefit to the patient.05-03-2012
20100233750Human Blood Brain Barrier Model - The present invention relates to an immortalized human brain endothelial cell line that is useful as an in vitro model of the blood brain barrier.09-16-2010
20090280519BIOMARKERS FOR ASSESSING LIVER FUNCTION - A method for assessing liver function in an individual, which method comprises determining the level of methylarginine(s) (such as ADMA and/or SDMA) and the ratio of ischemia modified albumin (IMA):albumin ratio (IMAR) in the individual, thereby to assess liver function in the individual.11-12-2009
20110287469DEVICE FOR INVESTIGATION OF A FLOW CONDUIT - A device for investigation of a flow conduit comprising: a base; and a module formed in the base, the module comprising: a main channel for the flow conduit, the main channel having a loading inlet for loading the flow conduit; a culture chamber in the main channel for at least one of perfusion and superfusion of the flow conduit; at least two fixation lines in communication with the main channel for providing fixation of the flow conduit at at least two fixation locations along the length of the flow conduit.11-24-2011
20110287471System and Method of Producing Volatile Organic Compounds from Fungi - An isolated fungus is described. The isolated fungus produces at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1,4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone. A method for producing at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1,4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone is also described. The method includes culturing a fungus on or within a culturing media in a container under conditions sufficient for producing the at least one compound.11-24-2011
20110287472MODULAR SYSTEM OF FUNCTIONAL UNITS FOR MIXING, PROCESSING AND/OR SEPARATING SAMPLES FOR USE IN BIOLOGICAL/MEDICAL RESEARCH AND FOR DIAGNOSTICS - Mixing, physical and/or chemical reactions and separation are basic method steps in biological research and diagnosis. In research in particular, there is a need for problem-specific laboratory systems, but these are not commercially available because of small batch sizes and because of the specific problem involved. In the absence of these systems, existing vessels, filters, centrifuges, etc., have to be improvised in order to solve the problem.11-24-2011
20110287470CELL CULTURE METHOD TO FORM AGGREGATES - The invention relates to the field of cell and tissue culture. In particular, the invention provides methods for culturing cells to form aggregates, including stem cells and primary cells. A method for culturing cells according to the invention comprises the steps of: (i) incubating a cells in a hanging drop on the underside of a porous membrane to form aggregates of cells; (ii) inverting the membrane so that the aggregates of cells are located on the upperside of the membrane; and (iii) incubating the aggregates of cells on the upperside of the membrane.11-24-2011
20110143389CELL ANALYSIS APPARATUS AND METHODS - Particular embodiments of the inventive technology relate to ‘off-axis detector’ technology that employs a third detector 06-16-2011
20110143388METHOD OF PROVIDING PORTABLE BIOLOGICAL TESTING CAPABILITIES - A method for providing portable biological testing capabilities free from biological contamination from an environment outside the device is provided. The method includes providing components configured to be assembled together to seal a volume against passage of biological materials between the volume and an environment outside the volume. The method further includes sterilizing the components and providing a sterilized culture medium. The method further includes assembling the components together with the sterilized culture medium within the volume. The method further includes sterilizing the assembled components by elevating the temperature. The method further includes flowing gas from within the volume to the environment while at an elevated temperature. The method further includes reducing the temperature to be less than the elevated temperature while preventing gas from flowing from the environment to the volume, thereby creating a pressure within the volume which is less than a pressure outside the volume.06-16-2011
20110287473Novel Fluorescent Boron-Substituted Dipyrromethenes and Use Thereof for Diagnosis - The invention relates to novel fluorescent compounds derived from non-fluorinated dipyrromethene-boron, to a method for preparing same and to the use thereof for the fluorescent marking of biological molecules. The invention also relates to biological molecules marked with said fluorescent compounds, and to the use thereof in detection methods such as medical diagnosis methods. More particularly, the detection methods of the invention are particularly useful for diagnosing neurodegenerative diseases such as Alzheimer's disease.11-24-2011
20100055731KEX2 Cleavage Regions OF Recombinant Fusion Proteins - The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.03-04-2010
20090035802METHOD FOR DETECTION AND ENUMERATION OF CELL SURFACE MARKERS - Disclosed is a method for detecting and counting cell surface markers using a difference in fluorescence intensities. A fluorescent material having a relatively lower fluorescence intensity is conjugated with one marker and another fluorescent material having a relatively higher fluorescence intensity is conjugated with the other marker. The two markers can be sorted and counted individually and simultaneously from the fluorescence intensity difference.02-05-2009
20100209962Tetranor PGDM: A Biomarker of PGD2 Synthesis In Vivo - The present invention relates to a prostaglandin D08-19-2010
20110294157METHOD FOR MODIFYING THE PROPERTIES OF A FLUID BY IRRADIATION, AND SYSTEM FOR IMPLEMENTING SAME - Methods for modifying the physical, chemical and/or biological properties of a fluid by irradiation are provided. In one embodiment, a method comprising supplying a flow of a fluid to an irradiation chamber; focusing the flow of the fluid so as to create at least one fluid layer; and applying radiation to the fluid layer in a defined portion of the irradiation chamber thereby modifying at least one physical, chemical or biological property of the fluid layer is provided. Systems for the implementation of such methods are also provided.12-01-2011
20110294156NOVEL ANTI CXCR4 ANTIBODIES AND THEIR USE FOR THE TREATMENT OF CANCER - The present invention relates to a novel isolated antibody, or the derived compounds or functional fragments of same, capable of binding to CXCR4 but also of inducing conformational changed of the CXCR4 homodimers and/or heterodimers.12-01-2011
20110294155MODULATION OF AN ION CHANNEL OR RECEPTOR - This invention relates to a method of assaying a compound for its ability to modulate an ion channel or receptor type, the method comprising: a) providing a dynamic clamp in electrical contact with a biological cell (or part thereof) in which one or more ion channel or receptor types for providing a waveform are functional and in which one or more ion channel or receptor types for providing a waveform are either not present or not functional; b) causing the dynamic clamp to apply a signal simulating the function of at least one of the one or more ion channel or receptor types that are either not present or not functional in the biological cell (or part thereof) based on modulation of the ion channel or receptor types that are functional in the biological cell (or part thereof) to thereby provide the waveform at the biological cell (or part thereof); c) exposing at least one of the one or more functional ion channel or receptor types to a compound; and d) detecting modulation of the waveform at the biological cell (or part thereof), wherein modulation of the waveform is indicative of a compound that modulates the at least one functional ion channel or receptor types.12-01-2011
20110294154FLOW CHAMBER AND ANALYTE DETECTION METHOD - A flow chamber and method for detecting the presence of one more cell produced analytes under flow conditions. The flow chamber includes two compartments separated by a permeable membrane on which a plurality of cells may be positioned. The permeable membrane shields one or more analyte sensors positioned one compartment from the convective transport forces of a fluid flow within the other compartment to allow reliable and accurate detection of cell-produced analytes and determination of the concentration of cell-produced analytes.12-01-2011
20110294153REAGENT AND METHOD FOR DETECTION OF CARBOXYLIC ACIDS BY MASS SPECTROMETRY - Method and reagent for converting a carboxylic acid to a positively charged amide. The method and reagent facilitate positive ion mass spectral analysis of carboxylic acids.12-01-2011
20110294152CALIBRATION MATERIAL DELIVERY DEVICES AND METHODS - A device is described that includes: a first portion configured to be grasped by the hand of the user, and a second portion defining a reservoir containing a control material, wherein the control material contains a target analyte in a known or predetermined concentration. Related arrangements and methods are also described.12-01-2011
20110294151Fluorescently Labeled Calcium Phosphate Surfaces - Described herein are substrates composed of a base coated with a fluorophore-labeled calcium phosphate coating. The substrates are useful in culturing and studying the activity of a variety of cells. The substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.12-01-2011
20090053756METHOD FOR MONITORING AND PROMOTING THE NUTRITION AND WELL-BEING AS WELL AS THE PRODUCTIVITY OF ANIMALS - The invention concerns a method for determining a characteristic describing the intestinal microbiota for an animal and/or a human being, in which method an animal and/or a human being is fed with a fodder/food; the number of two or more microorganisms in the intestine is determined; and the characteristic describing the intestinal microbiota is calculated as a ratio of the number of microorganisms. The invention also concerns methods for monitoring, development and promoting the intestinal microbiota, the well-being of an animal and/or a human-being, the intestinal health, the nutrition of an animal and/or a human being, the productivity and/or fodder utilization ratio of an animal, as well as the use of the characteristic and the use of the method for developing a fodder, a nutriment, a fodder and nutritional additive, a preparation and a pharmaceutical supporting the well-being, and a nutritional program.02-26-2009
20080293090AUTOMATED SEMI-SOLID MATRIX ASSAY AND LIQUID HANDLER APPARATUS FOR THE SAME - An improved liquid handling machine capable of regulating the temperature of assay compounds in the automated preparation of culture trays for biological assays is disclosed. The machine includes a horizontally movable table positioned beneath a vertically movable head. The table is divided into a plurality of stations holding mixing trays, culture trays and reservoirs of liquid assay compound. The head holds a plurality of pipettes which aspirate and expel liquid to transfer and mix the assay compounds between the reservoir, the mixing trays and the culture trays upon coordinated movement of the head and the table as controlled by a microprocessor. Each station on the table has independent heating, cooling and temperature sensing elements for regulating the temperature of the liquid held in a tray or reservoir at the station. A device for automatically evaluating the results of the assay, such as by fluorescence, spectrophotometric or radioactive techniques is incorporated with the improved liquid handling machine.11-27-2008
20090298115Fluorescent Gold Nanocluster and Method for Forming the Same - The present invention discloses a fluorescent gold nanocluster, comprising: a dihydrolipoic acid ligand (DHLA) on the surface thereof, wherein the fluorescent gold nanocluster generates fluorescence by the interaction between the dihydrolipoic acid ligand and the nanocluster and the particle diameter of the fluorescent gold nanocluster is between 0.5 nm and 3 nm, wherein the wavelength of the emission fluorescence of the fluorescent gold nanocluster is between 400 nm and 1000 nm. In addition, the fluorescent gold nanocluster is used as bioprobes and/or applied in fluorescent biological label, clinical image as contrast medium, clinical detection, clinical trace, and clinical treatment etc.12-03-2009
20090155838Devices, systems and methods for the collection, stimulation, stabilization, and analysis of a biological sample - Devices, systems, methods and kits for the collection, stimulation, stabilization and analysis of biological samples, including blood samples, are disclosed. An embodiment of the invention includes a container having a side wall, a bottom wall and a closure member defining an internal compartment having arranged therein a partition defining and fluidly separating first and second chambers in the internal compartment, the first chamber positioned in association with the closure member to receive the biological sample; in which at least one wall is constructed of an elastically deformable material; in which the first chamber contains at least one stimulating agent; in which the second chamber contains at least one stabilizing agent; and in which the first and second chambers can be placed in fluid communication by a user without opening or otherwise compromising the fluid integrity of the internal compartment.06-18-2009
20100273203METHODS AND COMPOSITIONS FOR DETECTING METABOLITES - The present invention provides a metabolic profile, a database comprising a metabolic profile, a method for determining a metabolic profile, uses for a metabolic profile, and warfarin metabolites.10-28-2010
20100136599METHODS AND PRODUCTS RELATED TO THE IMPROVED ANALYSIS OF CARBOHYDRATES - The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information regarding the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation on protein function. Such methods are also provided. Methods are also provided to assess protein production processes, to assess the purity of proteins produced, and to select proteins with the desired glycosylation.06-03-2010
20100099135METHODS AND ASSAYS FOR ASSESSING THE QUALITY OF EMBRYOS IN ASSISTED REPRODUCTION TECHNOLOGY PROTOCOLS - The present invention provides methods and assays for assessing the presence, viability and/or developmental stage of an embryo. The present invention also provides method and assays for assessing the quality of an Assisted Reproduction Technology protocol.04-22-2010
20100143961Histone deacetylase inhibitors based on alpha-ketoepoxide compounds - Histone deacetylase is a metallo-enzyme with zinc at the active site. Compounds having a zinc-binding moiety, for example, an alpha-ketoepoxide group, such as an alpha-ketothio group, can inhibit histone deacetylase. Histone deacetylase inhibition can repress gene expression, including expression of genes related to tumor suppression. Accordingly, inhibition of histone deacetylase can provide an alternate route for treating cancer, hematological disorders, e.g., hemoglobinopathies, autosomal dominant disorders, e.g. spinal muscular atrophy and Huntington's disease, genetic related metabolic disorders, e.g., cystic fibrosis and adrenoleukodystrophy, or to stimulate hematopoietic cells ex vivo.06-10-2010
20100035297METHODS AND COMPOSITIONS FOR VASCULOGENIC POTENTIAL DETERMINATION - Methods and compositions to evaluate the therapeutic vasculogenic potential of a variety of cells including endothelial cells using a monolayer of pericytic cells are disclosed. Pericytic cells or mural cells including those isolated from adipose tissue, such as for example, adipose stromal cells (ASC) or adipose-derived stromal cells (ADSCs) provide suitable conditions that stimulate endothelial cells from different origins to modulate stable vascular network organization in an in vitro experimental setting. Compositions that contain premixed ASC and EC are suitable for testing a candidate agent's angiogenic or antiangiogenic potential.02-11-2010
20100035296COMPOUNDS AND METHODS FOR DETECTION OF CELLS - The invention relates to compounds comprising an ester group for the detection in vivo of cells undergoing cell death (“dying cells”) such as, for example, cells undergoing apoptosis. These compounds are selectively retained in dying cells relative to normal cells. Thus, the compounds may be used in the detection, diagnosis and treatment of clinical conditions manifested by a cell death process.02-11-2010
20100035295Systems to analyze cellular metabolism and cell and molecular reactions - The present invention relates to systems capable of measuring an electrical signal produced by a cell, for example, by the metabolic activity of a cell. A system of the invention relates to varying the applied voltage and frequency to a culture vessel containing cells to account for the differences in cell metabolism of various and numerous cell types. A system of the invention, in certain embodiments, comprises a measurement board and a microprocessor. In certain other embodiments, the invention relates to methods for analyzing a cell or tissue using a system of the invention.02-11-2010
20110217724METHOD OF PROTECTING CELLS - Provided is a method for protecting stem cells in a clinical graft against destruction induced by the complement system by adding to the graft at least one factor capable of inhibiting the complement. Also provided is a method for protecting stem cells in a clinical graft against destruction induced by the complement system using a factor capable of inhibiting the complement.09-08-2011
20110217726METHOD AND KIT FOR DETECTING BIOLOGICAL SIGNAL OF THREE-DIMENSIONAL CELL CULTURE MATERIAL - Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.09-08-2011
20110217725CELL CULTURE KIT, SCREENING METHOD, AND METHOD OF MANUFACTURING CELL CULTURE KIT - To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (09-08-2011
20110217722PHOTO-DAMAGE APPARATUS AND METHODS FOR SORTING PARTICLES - A system and method for sorting a mixture of stained particles in a fluid flow path, including stained particles from two distinguishable groups. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path.09-08-2011
20110217721WATER SOLUBLE FLUORESCENT QUANTUM CARBON DOTS - The present invention relates to the water soluble self fluorescent quantum carbon dots (C-dots). These C-dots are isolated from carbon soot in one embodiment a wax soot solvent washed and isolated from other larger material by filtration such as by membrane filtration. The C-dots can be varied in their color by change of their size and by the amount of oxidative groups' position on each C-dot.09-08-2011
20090246820COMPOUNDS USEFUL IN CFTR ASSAYS AND METHODS THEREWITH - The present invention relates to compounds useful in CFTR assays. The present invention also relates to compounds useful in monitoring CFTR activity in therapies for CFTR-mediated diseases. The present invention also provides an assay for use in measuring CFTR correction.10-01-2009
20080311609Novel Molecular Probes - The present invention relates to novel molecular probes having the formula (I) (I) useful for the characterization, detection, localization and isolation of the γ-secretase enzyme.12-18-2008
20080305513METHOD OF MEASURING CANCER AGGRESSIVENESS AND DETECTION THEREOF - Erythrocyte sedimentation rate (“ESR”) determinations of a patient's blood that has been combined either with blood serum from pregnant mammals or with fetal embryonic serum yield measurable results correlating well with the presence of an on-going cancer. This cancer detection method is based on differential ESR determinations and uses paired in vitro ESR tests, in which a patient's whole blood is combined with a control serum (in a control vial) comprising serum from non-pregnant mammal and with a test serum (in a test vial) comprising either fetal embryonic serum or serum from a pregnant mammal to measure differential ESR in the tested control vials. Cancer coefficient K is calculated based on the ESR measurements and is compared with a threshold value to determine whether the patient may be identified as possibly having an on-going malignancy. A kit for performing the cancer screening methodology is also provided.12-11-2008
20080311611METHODOLOGY FOR VERIFYING CARBON STORAGE IN SEAWATER - A method for verifying carbon storage in seawater to which a growth stimulant has been supplied for stimulating a bloom of nitrogen fixing organisms for enhancing carbon storage therein, comprises selecting a region of seawater including a surface mixed layer, a euphotic zone extending below the surface mixed layer, and a plurality of deeper zones; determining the effect of growth stimulant on the rate of nitrogen fixation and carbon transport in the region; and determining the amount of carbon stored at different depths and projected duration of carbon storage at each of the depths.12-18-2008
20080311612Functional Expression of Higher Plant Nitrate Transporters in Pichia Pastoris - The present invention relates to a system for functional expression of higher plant nitrate transporter (Nrt) genes in 12-18-2008
20080311610Method for the Identification of Macromolecule Targets of Analytes - There is provided a method for the identification of macromolecule targets of analytes such as drugs in biological samples comprising complex mixtures of macromolecules. A biological sample is contacted with one or more analyte and the mixture is resolved such that the analyte and its target are co-eluted and analyzed to identify analyte-target complexes.12-18-2008
20080311614METHODS FOR MEASURING PH IN A SMALL-SCALE CELL CULTURE SYSTEM AND PREDICTING PERFORMANCE OF CELLS IN A LARGE-SCALE CULTURE SYSTEM - The present invention is directed to methods/systems for measuring the pH of a cell culture medium in a small-scale system utilizing a pH-sensitive dye. The present invention is also directed to methods for predicting the performance of cells in a large-scale culture system.12-18-2008
20090023178Parkin Substrate and Assay - The invention provides in vitro, ex vivo, and in vivo assays for Parkin activity, in which Parkin-mediated ubiquitination of a Sept4 protein is measured. The assays may be used to screen for agents that modulate Parkin protein ligase activity.01-22-2009
20100233748Device with biological component and method of making to achieve a desired transfer function - An improved method for the design and development of high performance hybrid devices having biologically-derived and nonbiological components and the hybrid devices so-designed and developed. A desired transfer function is determined for the biologically-derived component or components. The organism from which the biologically-derived component is derived is subjected to various environmental variables as it is grown. Organisms providing biologically-derived components having the desired transfer function are identified. The biologically-derived component is thereafter developed from organisms force adapted to cause the biologically-derived component transfer function to reach a goal or an acceptable measure. The biological component is used in hybrid constructs that may be nanostructures, given the small size of the biological parts. In one specific embodiment, force-adapted chlorosomes of 09-16-2010
20090162886Screening Method of Glutathione-Increasing Substance - As a means for treating diseases such as neurodegenerative diseases, malignant tumors and infectious diseases, a method for screening a substance increasing glutathione is provided. According to this method, by contacting a test substance with a cell expressing a GTRAP3-18 protein, a substance decreasing the amount of expression of the GTRAP3-18 protein or inhibiting a function of the GTRAP3-18 protein is specified as a target substance.06-25-2009
20090317857Transformation of Algal Cells - Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.12-24-2009
20100035298Hepatocyte Bioreactor System For Long Term Culture of Functional Hepatocyte Spheroids - A rotating wall vessel is used as a culture vessel and bioreactor for the cultivation of hepatocytes in the form of spheroids to generate a culture with many properties of the intact liver. These properties include enzyme activity comparable to fresh cells and long-term maintenance of viability and cellular function for periods on the order of months. The cultures may be used to produce hepatocyte products, evaluate metabolism of an agent, propagate Hepatitis C virus and test agents as inhibitors of this virus. Thus, the culture system disclosed herein makes long term functional cultivation of human hepatocytes feasible.02-11-2010
20100035292MICROFLUIDIC DEVICE FOR HIGH-THROUGHPUT CELLULAR GRADIENT AND DOSE RESPONSE STUDIES - The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.02-11-2010
20090093014Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof - This invention features a method of identifying a compound useful for enhancing efficacy of a therapeutic agent. The method includes incubating a compound in blood cells; separating immune cells from erythrocytic cells; and determining the ratio of the concentration of the compound in the immune cells to the concentration of the compound in the erythrocytic cells; wherein the compound comprises a transportophore and a therapeutic agent, in which the transportophore is covalently bonded to the therapeutic agent via a bond or a linker.04-09-2009
20100112624METHOD FOR OPERATING A TISSUE PROCESSOR, AND TISSUE PROCESSOR - A method for operating a tissue processor and a respective tissue processor for performing this method are described for the processing tissue samples. The tissue processor comprises at least one retort for receiving the tissue samples and at least one container for receiving a process medium. The process medium is transferred at least one of from the container into the retort and from the retort into the container. A value is automatically measured in the course of transferring the process medium, the value representing a characteristic property of the process medium. The process medium is identified based on the value.05-06-2010
20100112626Use of Semenogelin in the Diagnosis, Prognosis and Treatment of Cancer - Method of diagnosing cancer in a mammal comprising assaying a test sample from the mammal for an increased level of semenogelin, wherein the increased level of semenogelin in the test sample is diagnostic for the cancer; methods of prognosticating and assessing the effectiveness of treatment of a cancer in a mammal based on the level of semenogelin in a test sample; a composition comprising (a) an immune-response inducing effective amount of (i) semenogelin or polypeptide fragment thereof or (ii) antibody thereto or (b) a recombinant vector encoding and expressing an immune-response inducing effective amount of (i) or (ii); a method of inducing an immune response to a cancer in a mammal comprising administering to the mammal the foregoing composition.05-06-2010
20100112627System and Method for Displaying Three-Dimensional Object Scattergrams - System and method for displaying three-dimensional object scattergrams of particles are provided. In one embodiment, at least two parameters associated with at least one particle in a biological sample are detected and stored as data. An initial two-dimensional scattergram of the data is created with the two dimensions corresponding to the two parameters, and each data point corresponding to a particle in the biological sample. A data point in the initial two-dimensional scattergram is categorized into a population corresponding to a particle population. A density value of the data point is evaluated. Color data for the data point is calculated based on the evaluated density value and the categorized population. A three-dimensional location is generated based on the location in the initial two-dimensional scattergram and a property of the data point. A geometric shape centered at the generated three-dimensional location is displayed using the calculated color data.05-06-2010
20100196947Polyelectrolyte Multilayer Films At Liquid-Liquid Interfaces - The present invention is directed to methods for providing a polyelectrolyte multilayer film at a liquid-liquid interface. Such methods include steps of sequentially-depositing layers of cationic and anionic polyelectrolytes at a liquid-liquid interface that is formed between immiscible first and second liquids whereby a polyelectrolyte multilayer film is provided at the liquid-liquid interface. In certain preferred embodiments, the first liquid is an aqueous solution and the second liquid is a liquid crystal. In alternative embodiments, the first liquid is an aqueous solution and the second liquid is an oil. The invention further encompasses polyelectrolyte multilayer films provided by the disclosed methods as well as applications utilizing such materials.08-05-2010
20090081720Methods for identifying stem cells based on nuclear morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed.03-26-2009
20110129864METHOD FOR ANALYZING LYMPH NODE ASPIRATE USING MULTI-ANGLE LIGHT SCATTER FLOW CYTOMETER - Disclosed is a method for identifying lymphatic disease and disease states in mammals. The method uses a multi-angle light scatter flow cytometer to diagnose and treat mammals. The method includes collecting lymph node aspirate from a mammal; scanning the lymph node aspirate in a flow cytometer to generate a diagnostic scan; comparing the diagnostic scan to a known normal scan; identifying differences between the diagnostic scan and the known normal scan; and identifying similarities between the diagnostic scan and known disease scans to identify a cause of lymphadenopathy. Graphical representations of leukocyte identification are generated as a result of the scanning process. By using the graphs, a veterinarian or technician is able to diagnose the effectiveness or ineffectiveness of the treatment.06-02-2011
20100028933NUTRIENT MEDIUM UNIT AND METHOD FOR HOLDING A FILTER FROM A FILTRATION DEVICE - The invention relates to a nutrient medium unit for holding a filter of a filtration device, comprising a lower part that is filled with the nutrient medium and a lid, the latter having a fixing edge that protrudes into the lower part and that can be connected to an edge of the filter by means of an adhesive bond in order to remove said filter from the filtration device. The invention also relates to a method for the microbiological analysis of liquid samples, according to which a membrane filter is lifted off a filter support and laid on the surface of a nutrient medium that is situated in a lower part of a nutrient medium unit. The lower part is then covered by a lid, the latter being placed on the membrane filter lying on the filter support in such a way that a fixing edge located in the lid is connected to an edge of the filter by means of an adhesive bond. The lid and the attached filter are lifted off the filter support and placed on the dish-shaped lower part of the nutrient medium unit.02-04-2010
20090148884LAT1 TRANSPORTERS EXPRESSED IN BLOOD BRAIN BARRIER CELLS - LAT1 is consistently expressed at high levels in brain microvessel endothelial cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is actively transported by, the LAT1 transporter, and therefore a candidate substrate for crossing the blood brain barrier. The assays are useful in screening for therapeutic, cytotoxic or imaging compounds used in the treatment or diagnosis of neurological diseases.06-11-2009
20090148882MULTIPLE COAGULATION TEST CARTRIDGE AND METHOD OF USING SAME - Embodiments of the present invention relate to multiple coagulation test cartridges and methods of using such cartridges. In one embodiment the cartridge is a disposable single-use cartridge for use in evaluating blood clotting. The cartridge includes multiple containers, such as tubes, each of which includes one or more coagulation affecting substances. The containers can be pre-filled with substances in amounts suitable for use with a single patient's blood sample. The cartridge may include one or more containers, each of which has multiple sections, or volumes, each section storing a different coagulation affecting substance. In another embodiment the cartridge is used in a method for determining at least one appropriate coagulation affecting substance for modifying a patient's coagulation status using a multiple coagulation test system.06-11-2009
20090148880METHOD AND DEVICES FOR TREATING INDIVIDUAL BIOLOGICAL CELLS - The invention relates to a method for treating a biological cell (06-11-2009
20090047701METHOD FOR SCREENING ANTIFUNGAL AGENTS - The present invention relates to a fungus which produces substantially no functional protein with regulating activity on the transcription of genes which are involved in cell wall stress response. It also relates to a method for screening for antifungal agents, in particular to antifungal agents which target the cell wall. It also relates to a kit for screening for antifungal agents which disturb cell wall biogenesis.02-19-2009
20100267078Selective Detection of Proteins that Contain Two or More Alpha-Helical Transmembrane Domains - Embodiments of the present invention provide a staining solution and of method of using the staining solution for selectively detecting proteins that contain two or more α-helical transmembrane domains. The staining solution comprises a lipophilic dyes and at least about a 30% hydrophobic solvent. The dyes of the present are represented by the general formula A-B-E wherein A is a nitrogen heterocycle, B is a bridge moiety and E is an electron pair accepting moiety that comprises either a carbonyl or nitrogen atom. In one embodiment these lipophilic dyes are merocyanine dye, a cyanine dye, a styryl dye or a carbazolylvinyl dye.10-21-2010
20100267073STEM CELL-BASED CULTURE SYSTEM FOR DRUG DEVELOPMENT - The present invention relates to culture systems comprising differentiated stem cells, that may be used for identifying agents useful in treating degenerative nervous system disorders and are suitable for high-throughput screening applications. It is based, at least in part, on the discovery that co-cultures of (i) astrocytes expressing a mutated SODI gene and (ii) stem-cell derived motor neurons manifested cell death via a Bax-dependent mechanism, and modeled motor neuron death in amyotrophic lateral sclerosis.10-21-2010
20100267076Separation of Co-Cultivated Cell Populations - The invention relates to a method and the use of an apparatus for separating co-cultivated cell populations.10-21-2010
20100267074MEASUREMENT AND USES OF OXIDATIVE STRESS - The present invention provides a method of determining the overall oxidative status of a body fluid or a tissue of a patient by measuring the oxidation-reduction potential (ORP) of the body fluid or tissue. The method has been found to be useful in the diagnosis, evaluation and monitoring of patients who have suffered a trauma (such as a head injury), patients suspected of being critically-ill or who are critically ill, patients who have an infection, and patients suspected of having a myocardial infarction (MI) or who have had an MI. The method has also been found useful in monitoring and evaluating exercise performance in patients. In addition, the method has been found useful in monitoring and evaluating stored blood products and patients who will receive such a product.10-21-2010
20100267072Formation/Elongation of Axon by Inhibiting the Expression or Function of Singar and Application to Nerve Regeneration - Singar is identified as a novel molecule, whose expression is varied before and after the polarization of a nerve cell, and occurs in the tip of an elongating axon, called a growth cone, which is important for the formation or elongation of an axon. Singar is expressed specifically in the brain and the amount of Singar expression is largely increased in an individual during periods where the formation of axons is increased. It is observed that Singar is highly enriched in the growth cone at the tip of an axon. When the expression of Singar is inhibited in nerve cells in culture, the formation of multiple axons is induced. Thus, the inhibition of Singar can induce axon formation. Therefore, by inhibiting the expression or activity of Singar, it becomes possible to induce or promote the formation or elongation of an axon in a nerve cell.10-21-2010
20100055732Cardiac Hypertrophy - The invention provides means and methods for at least in part inducing, counteracting, preventing and/or investigating cardiac hypertrophy.03-04-2010
20110262956CO-CULTURE COMPOSITIONS AND METHODS - Co-culture compositions and methods are described for identifying agents that modulate a cellular phenotype, particularly of neurons or pancreatic beta cells are provided herein, where the methods include co-culturing differentiated cells, wherein at least one of the cell-types are derived from human induced pluripotent stem cells from a subject having or predisposed to a neurodegenerative or metabolic disorder. Co-culture compositions of differentiated cells from two different human subjects are also described.10-27-2011
20120045790MICRODISSECTION METHOD AND INFORMATION PROCESSING SYSTEM - In a first aspect, a method for use in biology, histology, and pathology, comprises: providing a digital first image (02-23-2012
20090155832Apparatus and Method for Improved Optical Detection of Particles in Fluid - A number of fluidic-photonic devices for allowing optical detection, systems employing such devices, and related methods of operation and fabrication of such devices are disclosed herein. In at least some embodiments, the devices can serve as flow cytometry devices and/or employ microfiuidic channels. Also, in at least some embodiments, the devices are fluidic-photonic integrated circuit (FPIC) devices that employ both fluidic channels and one or more waveguides capable of receiving and/or delivering light, and that can be fabricated using polymeric materials. The fluidic-photonic devices in at least some embodiments are capable of functionality such as on-chip excitation, time-of-flight measurement, and can experience enhanced fluorescence detection sensitivity. In at least some embodiments, the devices employ detection waveguides that are joined by way of a waveguide demultiplexer. In additional embodiments, a variety of techniques can be used to process information received via the waveguides, including an iterative cross-correlation process.06-18-2009
20100086959FILTRATION UNIT AND METHOD FOR THE MICROBIOLOGICAL ANALYSIS OF LIQUID SAMPLES - The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane. The cover is lifted off the filter support together with the membrane and placed on the bowl-shaped bottom part such that the bottom of the membrane facing away from the cover rests on the top of the nutrient medium facing the cover.04-08-2010
20120034643FLUORESCENT PROTEIN - An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from 02-09-2012
20120034644IDENTIFYING MATERIAL FROM A BREAST DUCT - Methods and systems for identifying material from a breast duct using one or more markers that can be identified in ductal fluid retrieved from the breast are provided.02-09-2012
20100015654Negative regulation of NK cell functions by EAT-2, a sap-related adaptor expressed in innate immune cells - The present invention relates to the identification EAT-2 and ERT as novel therapeutic targets for the modulation of innate immune cell functions. More particularly the present invention describes novel methods for modulating innate immune cells-mediated immune response, useful in the treatment of cancer, infectious diseases as well as autoimmune diseases. The invention also features EAT-2 deficient and overexpressing transgenic animals, screening assays to identify agents that modulate EAT-2 and ERT activity or expression as well as methods of treatments comprising a modulation of NK cells function01-21-2010
20110027819USE OF SFRP-3 IN THE ASSESSMENT OF HEART FAILURE - Disclosed is a method for assessing heart failure in vitro including the steps of measuring in a sample the concentration of the marker SFRP-3, of optionally measuring in the sample the concentration of one or more other marker(s) of heart failure, and of assessing heart failure by comparing the concentration determined in for SFRP-3 and the concentration(s) determined for the optionally one or more other marker to the concentration of this marker or these markers as established in a reference population. Also disclosed are the use of SFRP-3 as a marker protein in the assessment of heart failure, a marker combination comprising SFRP-3 and a kit for measuring SFRP-3.02-03-2011
20100285519QUINOLINONE-CARBOXAMIDE COMPOUNDS AS 5-HT4 RECEPTOR AGONISTS - The invention provides novel quinolinone-carboxamide 5-HT4 receptor agonist compounds of Formula (I). The invention also provides pharmaceutical compositions comprising such compounds, the use such compounds to treat diseases associated with 5-HT4 receptor activity, and processes and intermediates useful for preparing such compounds. Wherein; R11-11-2010
20110136163Method of Toxicological Assessment - A method of assessing toxicity of a candidate agent to a sample of cells comprises the steps of providing a sample of cells, exposing the cells to the candidate agent for a suitable period of time, assaying the cells to measure data for at least one parameter of cellular function; and correlating the measured data of the at least one parameter of cellular function with toxicity, wherein the step of exposing the cells to the candidate agent is carried out in the presence of a reagent capable of facilitating transport of the candidate agent into the cell. The transport reagent may be an endocytosis, pinocytosis inducing agent, a peptide, or a liposome. The at least one parameter of cellular function may be selected from the group consisting of: cell viability; proliferation rate; membrane integrity; and a metabolic parameter. Also described is a method of generating a toxicity signature for a candidate agent comprising the step of carrying out the method of the invention for a plurality of cellular function parameters, and compiling the measured data for each of the cellular function parameters to provide a toxicity signature.06-09-2011
20090087876METHODS AND COMPOSITIONS USEFUL FOR MODULATING DRUG-INDUCED IMPAIRMENT - The present invention provides isolated nucleic acids, polypeptides, oligonucleotides, vectors, host cells, antibodies, compositions, and kits relating to happyhour. Also provided are methods of screening for agents capable of modulating happyhour activity.04-02-2009
20090087875Alzheimer's related proteins and methods of use - Disclosed is a method for identifying substances that alter the interaction of a presenilin protein with a presenilin-binding protein, including contacting at least the interacting domain of a presenilin protein to a presenilin-binding protein in the presence of a test substance, and measuring the interaction of the presenilin protein and the presenilin-binding protein. Also disclosed is method for identifying substances that modulate the nuclear translocation of an armadillo protein, including providing a culture of cells that express the armadillo protein and a mutant presenilin protein, or a functional fragment thereof that binds an armadillo protein; contacting the culture with a test substance; inducing nuclear translocation of the armadillo protein in the cells; and measuring levels of nuclear armadillo protein as compared to a control as an indication of modulatory activity of the test substance. Further disclosed is method for screening individuals for presenilin alleles associated with Alzheimer's Disease or related disorders, including obtaining cells from an individual to be tested for Alzheimer's Disease or a related disorder; inducing nuclear translocation of an armadillo protein in the cells; and measuring levels of the nuclear armadillo protein as compared to a control as an indication of the presence or absence of presenilin alleles associated with Alzheimer's Disease or a related disorder.04-02-2009
20120142043METHOD FOR AUTOMATIC DETERMINATION OF SAMPLE - A method of determining a kind of a sample, in a method for analyzing a substance by the steps of supplying the sample to be analyzed to a reaction system by a supplying means comprising a transparent region composed of a transparent material, reacting a reagent for detecting the substance with the sample in the reaction system, and analyzing a signal derived from a product obtained by the reaction, characterized by irradiating the transparent region with light in the supplying step, and analyzing an optical intensity of the light.06-07-2012
20100143958Detection of Soluble Adiponectin Receptor Peptides and Use in Diagnostics and Therapeutics - The present invention relates to soluble C-terminal fragments of the adiponectin receptor and their use in the diagnosis and management of disorders.06-10-2010
20100190200Diagnosis of Gynecological Neoplasms By Detecting The Levels of Oviduct-Specific Glycoprotein - The present invention provides a method for detecting cancer in a patient. A sample from the patient is provided, and the level of oviduct-specific glycoprotein (OGP) in the sample is determined and compared to a control sample. Increased levels of OGP in the sample as compared to the control indicates that the patient has cancer. In one aspect, the cancer is a gynecological cancer, such as ovarian cancer. Kits for conducting the methods of the invention are also provided.07-29-2010
20100190202NOVEL POPULATION OF HEPATOCYTES DERIVED VIA DEFINITIVE ENDODERM (DE-HEP) FROM HUMAN BLASTOCYSTS STEM CELLS - The present disclosure relates to a novel hepatocyte-like cell progenitor and/or a novel hepatocyte-like cell derived via definitive endoderm from human blastocyst-derived stem (hBS) cells, to a method for the preparation of such cells and to the potential use of such cells in, e.g., pharmaceutical drug discovery and development, toxicity testing, cell therapy and medical treatment. In particular is presented a definitive endoderm derived hepatocyte-like cell with important liver-expressed marker genes and important metabolizing enzymes, as well as drug transporters.07-29-2010
20090053752Use of an in vitro hemodynamic endothelial/smooth muscle cell co-culture model to identify new therapeutic targets for vascular disease - An in vitro biomechanical model used to applied hemodynamic (i.e., blood flow) patterns modeled after the human circulation to human/animal cells in culture. This model replicates hemodynamic flow patterns that are measured directly from the human circulation using non-invasive magnetic resonance imaging and translated to the motor that controls the rotation of the cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the surface of the cells that are grown on the plate surface. The rotation of the cone transduces momentum on the fluid and creates time-varying shear stresses on the plate or cellular surface. This model most closely mimics the physiological hemodynamic forces imparted on endothelial cells (cell lining blood vessels) in vivo and overcomes previous flow devices limited in applying more simplified nonphysiological flow patterns. Another aspect of this invention is directed to incorporate a transwell co-cultured dish. This permits two to three or more different cell types to be physically separated within the culture dish environment, while the inner cellular surface is exposed to the simulated hemodynamic flow patterns. Other significant modifications include custom in-flow and out-flow tubing to supply media, drugs, etc. separately and independently to both the inner and outer chambers of the coculture model. External components are used to control for physiological temperature and gas concentration. The physical separation of adherent cells by the artificial transwell membrane and the bottom of the Petri dish permits each cell layer, or surface to be separately isolated for an array of biological analyses (i.e., protein, gene, etc.).02-26-2009
20100081163ION CHANNEL ASSAY METHODS - A method of characterizing the biological activity of a candidate compound may include exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.04-01-2010
20090280520FLUORESCENT SEMICONDUCTOR PARTICLES, METHOD OF MANUFACTURING THE SAME, BIOSUBSTANCE FLUORESCENT LABELING AGENT EMPLOYING THE SAME, AND BIOIMAGING METHOD THEREOF - Disclosed is a fluorescent semiconductor particle having a core/shell structure composed of a core particle as a semiconductor particle, and a shell layer by which the core particle is covered, wherein the core particle has a different chemical composition from that of the shell layer; the core particle has an average particle diameter of 1-15 nm and a specific gravity of 1.0-3.0; and the fluorescent semiconductor particle having the core/shell structure has a specific gravity of 0.8-3.211-12-2009
20100081164BIOCHEMICAL MARKERS FOR ACUTE PULMONARY EMBOLISM - The present invention relates to a method of differentiating between a singular and a multiple lung embolism in a subject suspected to suffer from acute lung embolism comprising determining the amount of NT-proBNP in a sample of a subject suspected to suffer from acute lung embolism and comparing the amount to a reference amount. Further, the present invention also relates to a method of differentiating between acute and chronic lung embolism in a subject comprising determining the amount of NT-proANP at a first and a second time point and comparing the determined amounts with each other. The present invention also encompasses devices and kits for carrying out the aforementioned methods.04-01-2010
20120107861METHOD OF DETERMINING RISK OF ARRHYTHMIA - The present invention relates to a method of determining the risk of drug induced arrhythmia using stem cell derived cardiomyocytes in a high-throughput impedance or multi-electrode array assay.05-03-2012
20120107860CELL SORTING APPARATUS, CELL SORTING CHIP, AND CELL SORTING METHOD - Disclosed herein is a cell sorting apparatus, including: a branch portion branching a flow path in which a fluid containing therein cells flows into a first branch flow path, and a second branch flow path; a coupling portion coupling the first branch flow path and the second branch flow path to each other; and a flowing-out portion causing liquids flowing in the first branch flow path and the second branch flow path coupled to each other by the coupling portion, respectively, to flow out to an outside.05-03-2012
20090263856Apparatus and Method for Detecting Live Cells with an Integrated Filter and Growth Detection Device - A device for rapid concentration and detection of live cells in fluids includes a filter to capture a cell sample. The filter includes a first physical barrier with apertures of a first size and a second physical barrier with apertures of a second size smaller than the first size to isolate the cell sample on the filter. Growth detection circuitry associated with the filter electrically measures a cell growth rate associated with the cell sample in less than 2 days. The growth detection circuitry includes a mechanical filter for concentration of cells. The filter and growth detection circuitry are integrally formed within the device, which is sealed.10-22-2009
20090263853High-Throughput Cell-Based CFTR Assay - This invention provides a high throughput screen for measuring the transport of an ion through a cystic fibrosis transmembrane conductance regulator (CFTR) of a cell that endogenously expresses CFTR. The method requires culturing the cell that endogenously expresses CFTR in the presence of an ion-sensitive compound and then contacting the cell with a CFTR activator. After a suitable amount of time after addition of the activator, the test compound is added to the cell culture medium. Thereafter, any change in the ion-sensitive compound is measured by any suitable method that can detect the change in the ion-sensitive compound, i.e. by measuring a change in the light emitted from a fluorescent compound, which can be recorded by an imaging plate reader.10-22-2009
20090263854Integrated assay device and housing - A laminated, integrated, analyte assay device and methods of using the device are described. The assay device is useful as an inexpensive, disposable assay device for detecting the presence and/or amount of a particular analyte in a body fluid or other sample.10-22-2009
20090263849Bioprinting Three-Dimensional Structure Onto Microscale Tissue Analog Devices for Pharmacokinetic Study and Other Uses - A microfluidic system for monitoring or detecting a change in a parameter of an input substance, which includes a microfluidic device having a tissue chamber and a tissue analog placed in the tissue chamber, wherein the tissue analog has a vessel structure mimicking naturally occurring vessel network incorporated in the tissue analog.10-22-2009
20090263851IN VIVO SELECTION SYSTEM FOR ENZYME ACTIVITY - The present invention provides in vivo systems in which activity of a biological cleavage enzyme, such as a site-specific recombinase, a homing endonuclease, or an intein, is linked to cell viability and therefore can be selected. The invention further provides methods of making cells in which the activity of a biological cleavage enzyme is linked to viability, as well as methods of identifying new biological cleavage enzymes, including enzymes having altered site specificity, using such cells.10-22-2009
20090263848Method for Determining the Viability of Cells in Cell Cultures - The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10-22-2009
20090263847LSR RECEPTOR, ITS ACTIVITY, ITS CLONING AND ITS APPLICATIONS TO THE DIAGNOSIS, PREVENTION AND/OR TREATMENT OF OBESITY AND RELATED RISKS OR COMPLICATIONS - The present invention relates to a new complex receptor polypeptide LSR (Lipolysis Stimulated Receptor), characterized by its functional activities, the cloning of the cDNAs complementary to the messenger RNAs encoding each of the subunits of the multimeric complex, vectors and transformed cells, methods of diagnosis and of selection of compounds which can be used as medicament for the prevention and/or treatment of pathologies and/or of pathogeneses such as obesity and anorexia, hyperlipidemias, atherosclerosis, diabetes, hypertension, and more generally the various pathologies associated with abnormalities in the metabolism of cytokines.10-22-2009
20090263850METHOD AND SYSTEM FOR MEASURING SINGLE CELL MECHANICS USING A MODIFIED SCANNING PROBE MICROSCOPE - One embodiment of the present invention provides a system that measures single cell mechanics using a scanning probe microscope. During operation, the system positions a modified probe of the scanning probe microscope above a cell which is located on a surface, wherein the modified probe is configured with a geometry for compressing the cell. The system then comprises the cell against the surface using the modified probe, thereby causing the cell to deform. Next, the system extracts mechanical properties of the cell from cell deformation behavior and cell response to the compression force.10-22-2009
20090263855Compositions and Methods For Preventing or Treating Inflammatory Bowel Disease - Compositions comprising docosahexaenoic acid (DHA) and optionally one or more fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), arachidonic acid (ARA), linoleic acid (LA), and α-linoleic acid (ALA) are administered to felines for preventing or treating feline inflammatory bowel disease (IBD).10-22-2009
20130217060METHOD FOR THE PRODUCTION OF 1-BUTANOL - A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.08-22-2013
20100124761METHOD AND DEVICE FOR MEASURING EXTRACELLULAR ACIDIFICATION AND OXYGEN CONSUMPTION RATE WITH HIGHER PRECISION - The accuracy and/or precision of measurements of extracellular acidification rate or CO05-20-2010
20100099130METHODS AND DEVICES FOR MONITORING PLATELET FUNCTION - A method of monitoring platelet function comprising: passing blood removed from a mammal through a passageway comprising (i) a shear generating restriction to generate a platelet mass in the passageway and (ii) a platelet aggregate trap; and monitoring the flow or composition of the blood in the passageway to detect formation of the platelet mass, wherein the blood passes through the passageway in one direction and only one time. A method of monitoring platelet function comprising: passing blood removed from a mammal through a passageway comprising (i) a shear generating restriction to generate a platelet mass in the passageway and (ii) a platelet aggregate trap; and monitoring the flow or composition of the blood in the passageway to detect formation of the platelet mass, wherein the blood is recirculated through the passageway and the blood flows only one direction through the passageway.04-22-2010
20100099133METHOD AND APPARATUS FOR THE TREATMENT OF SPECIMENS - A method and an apparatus for the treatment of cytological and histological specimens are described. A treatment program comprising multiple treatment parameters is predetermined. The specimens are introduced in accordance with the predetermined treatment program sequentially into a plurality of treatment stations by means of a transport device. At least one of the process data time and number of specimens subject to simultaneous treatment in one particular treatment station and number of the treatment stations during execution of the treatment program are determined. These process data are evaluated for optimizing the treatment program as to at least one of processing time, number of treatment stations and sequence of treatment stations.04-22-2010
20090203061DEVICE AND METHOD FOR OBSERVING THE ORGANIZATION OF STUDIED CELLS CULTURED IN THE PRESENCE OF A CONCENTRATION GRADIENT OF CHEMOTACTIC MOLECULES - The device comprises a cell culture compartment (08-13-2009
20110201042Inhibitors of Bacterial Nitric Oxide Synthase, and Related Screening Methods - The invention relates in part to compounds that act as highly nitric oxide (NO)-specific turn-on fluorescent probes. The present invention also relates to the use of these fluorescein-based sensors to screen selectively for inhibitors of bacterial nitric oxide synthase (bNOS). Compounds of the present invention readily detect nitric oxide produced in vivo. Therefore they can be used in an assay that measures NO production by bNOS. Using a sensor of the invention one can screen libraries of small molecules for inhibitors of bNOS.08-18-2011
20110201040NON-MCCJ25-RELATED LARIAT-PEPTIDE INHIBITORS OF BACTERIAL RNA POLYMERASE - The invention provides a method of inhibiting a bacterial RNA polymerases. The invention has applications in control of bacterial RNA polymerase activity, control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.08-18-2011
20100323383Methods for in vitro differentiation of Th-17+cells - The present invention is directed to an in vitro method for promoting differentiation and proliferation of human T helper lymphocytes that express IL17 (Th-IL17+ cells).12-23-2010
20100124760Zinc Test Strip and Method for the Detection of Semen - The present invention relates to a forensic test strip and method for the detection of semen. This strip is comprised of a paper element coated with reagents which react to the presence of zinc, a component of semen, and is affixed to a plastic backing to facilitate testing items without touching the highly sensitive coated portion of the strip. This assembly is exposed to a source of semen, such as a suspicious stain on a garment. A positive test is characterized by a bright pink color, which easily can be seen against the light yellow background. A positive test provides presumptive evidence of semen.05-20-2010
20090275070Compounds and Methods of Identifying, Synthesizing, Optimizing and Profiling Protein Modulators - This invention relates to methods of identifying, synthesizing, optimizing and profiling compounds that are inhibitors or activators of proteins, both naturally occurring endogenous proteins as well as certain variant forms of endogenous proteins, and novel methods of identifying such variants. The method accelerates the identification and development of compounds as potential therapeutically effective drugs by simplifying the pharmaceutical discovery and creation process through improvements in hit identification, lead optimization, biological profiling, and rapid elimination of toxic compounds. Implementation results in overall cost, reductions in the drug discovery process resulting from the corresponding increases in efficiency.11-05-2009
20090142793SCREENING ASSAY TO IDENTIFY MODULATORS OF THE SLEEP/WAKE CYCLE - Screening assays for identifying agents that modulate BK channel activity and further modulate the sleep/wake cycle in a subject, circadian regulated locomotor activity in a subject, or both are provided, as are agents identified using such screening assays. Also provided are methods of modulating the sleep/wake cycle in a subject and methods of modulating circadian regulated locomotor activity in a subject by administering an agent that modulates BK channel activity to the subject, for example, an agent identified by a screening assay as disclosed.06-04-2009
20090280521Methods for Drug Discovery, Disease Treatment, and Diagnosis Using Metabolomics - The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.11-12-2009
20110171680BUFFY COAT TUBE AND FLOAT SYSTEM AND METHOD - A tube and float system for use in separation and axial expansion of the buffy coat is provided. The system includes a transparent, or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The sample tube has an elongated sidewall having a first cross-sectional inner diameter. The float consists of a main body portion and one or more support members protruding from the main body portion to engage and support the sidewall of the sample tube. The main body portion and the support members of the float have a cross-sectional diameter less than that of the first cross-sectional inner diameter of the tube when the sample tube is expanded, such as by centrifugation. The main body portion of the float together with an axially aligned portion of the sidewall define an annular volume therebetween. The support members protruding from the main body portion of the float traverse said annular volume to produce one or more analysis areas. During centrifugation, the centrifugal force enlarges the diameter of the tube to permit density-based axial movement of the float in the tube. Thereafter, the centrifugal force is reduced to cause the tube sidewall to return to its first diameter, thereby capturing the float and trapping the buffy coat constituents in the analysis area. The bully coat constituents can then be evaluated or measured.07-14-2011
20110171679L-GLUTAMIC ACID-PRODUCING MICROORGANISM AND A METHOD FOR PRODUCING L-GLUTAMIC ACID - A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.07-14-2011
20110171678MODIFIED CARBOCYANINE DYES AND THEIR CONJUGATES - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.07-14-2011
20110171677FLUORESCENCE DETECTION OF POISON OAK OIL - The invention herein disclosed provides for compositions, methods for synthesizing said compositions, and methods for using said compositions, wherein the compositions and methods may be used to bind to and/or deactivate a poison oak oil, such as urushiol. The compositions and methods can be used to treat and/or reduce an inflammatory reaction and/or hypersensitivity to natural compounds found in poison oak, poison ivy, poison sumac, mango, lac tree, and cashew nut.07-14-2011
20090093011Biosensors for ligand-directed functional selectivity - A system and method for determining ligand-directed functional selectivity of a receptor in a live-cell with a biosensor are disclosed. Also disclosed is a system and method for fragment-based screening with a cell-based, label-free biosensor functional assay.04-09-2009
20110269173Method for Producing Polarized Retinal Progenitor Cells from Pluripotent Stem Cells and their Differentiation into Retinal Pigment Epithelium Cells - The present invention relates to a method and components for producing polarized retinal progenitor cells (RPC) from pluripotent stem cells in high yield and purity. The polarized retinal progenitor cells are preferably further differentiated with high efficiency and speed into retinal pigment epithelium cells (RPE cells). The cells obtained are particularly suitable for use in cell transplantation or in the generation of transplant tissue and are particularly applicable to screening systems for substances that modulate the function of polarized retinal progenitor cells and/or RPE cells.11-03-2011
20080280318Isolation and Culture of a High Purity Population of Cone Photoreceptor Cells by Tissue Dissociation and Pna-Panning, and Biological Applications Thereof - The present invention provides a process and kit for isolating cone photoreceptor cells from retinal tissue with a purity level of at least 80%, typically of about 90%. The isolation process uses a PNA-panning procedure conducted on dissociated retinal tissue. The present invention also provides a culture medium enabling the in vitro survival and development of such isolated cone cells. The means of the invention are applicable to adult mammalian cone cells, and more particularly to adult human cone cells. They have the advantage of being applicable to pathologic or otherwise altered cone cells, and thus give access to the screening of compounds capable of showing neuroprotective activity on adult cone cells.11-13-2008
20090291464Methods for Measuring Affinity Substances in Samples Containing Blood Cell Components - In the methods for measuring affinity substances using pearl chain formation of carrier particles, the use of carrier particles having a particular particle diameter enables carrier particles to be specifically counted even if blood cell components coexist. Whole blood samples can be used as samples to be measured without separating serum and plasma. Therefore, there is no need to separate serum or plasma when analyzing blood cell components.11-26-2009
20100068749OPTICAL SENSOR AND METHOD FOR INDICATING THE AGE OR QUALITY OF A NATURAL PRODUCT - The present invention relates to the field of analyzing the age and/or quality of certain natural products, for example foods. The invention also relates to devices for analyzing said age and/or quality as well as to methods for preparing such devices, to methods for analyzing natural products and to their use.03-18-2010
20080206802Microinjection device and microinjection method - A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.08-28-2008
20080206803Metabolic genes and related methods and compositions - Certain aspects of the invention provide nucleic acid constructs that can be used to cause a cell to be dependent on a particular enzymatic activity or on the presence of a particular small molecule. Certain aspects of the invention also provide methods for cloning genes involved in the synthesis, modification or degradation of a given molecule and for the directed evolution of proteins that perform a specified enzymatic function. Certain methods of the invention can be used to isolate the genes responsible for directing the biosynthesis, modification or degradation of a particular target molecule and to isolate polypeptide variants having new or improved enzymatic activity.08-28-2008
20080206806Method for Evaluating or Screening Hair Growth-Regulating Agent - The present invention provides a method for evaluating or screening a hair growth-regulating agent which utilizes a readily available animal.08-28-2008
20080206805Compounds and Related Methods for Mutant p53 Reactivation - Ketoamine compounds and related methods for reactivation of tumor suppressor protein p53.08-28-2008
20080206801BIOLOGICAL INDICATOR FOR USE WITH VAPOROUS MICROBIAL DEACTIVATING AGENTS AND METHOD FOR MAKING SAME - A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent.08-28-2008
20080206804ARRANGEMENTS AND METHODS FOR MULTIDIMENSIONAL MULTIPLEXED LUMINESCENCE IMAGING AND DIAGNOSIS - Exemplary embodiments of arrangements and methods providing information associated with a sample are described. For example, using such exemplary arrangements and methods, it is possible to receive an unpartitioned electro-magnetic radiation from the sample. Further, first data associated with first luminescent characteristics of at least one first molecule of the sample and second data associated with second luminescent characteristics of at least one second molecule of the sample can be obtained based on the unpartitioned electro-magnetic radiation. At least two of the photo-luminescent properties of the sample may be measured simultaneously as a function of the first and second data. Further, the information regarding the molecules of the sample may be determined as a function of the photo-luminescent properties.08-28-2008
20110171681METHODS AND COMPOSITIONS FOR REGULATION OF STEM CELL SURVIVAL, PROLIFERATION, AND DIFFERENTIATION BY PROTEIN UBIQUITINATION - Compositions and methods for regulating in vitro cell growth are disclosed, and for providing undifferentiated stem cells or embryonic cells that are suitable for transplantation into damaged tissues or organs, or for use in tissue repair. A representative method includes causing the overexpression or underexpression of GalT binding protein (GtBP), also referred to as GalT associated protein (GTAP), in a cell such that ubiquitination of at least one cellular protein associated with cell adhesion and/or cell-to-cell interaction is correspondingly increased or decreased, causing inhibition of cell growth when GTAP is overexpressed and causing enhanced cell growth when GTAP is underexpressed by the cell. As a result, growth of the cell is altered or regulated.07-14-2011
20090104647METHODS FOR SELECTING ACTIVE AGENTS FOR CANCER TREATMENT - The present invention provides methods for individualizing chemotherapy, and particularly methods for individualizing neoadjuvant chemotherapy. The present invention provides methods for predicting a cancer patient's response to neoadjuvant chemotherapy, including assessing the probability of a positive response upon treatment with candidate agents prior to surgery. In various aspects, the invention involves culturing a monolayer of malignant cells from an explant of a patient's biopsy specimen, such as a transcutaneous biopsy-sized specimen, and testing the malignant cells for resistance or sensitivity to one or a plurality of candidate agents for neoadjuvant therapy. In other aspects, the invention provides methods for accurately scoring and interpreting such assays, and discloses in vitro chemoresponse results that are predictive of a patient's pathological complete response (pCR) upon receiving the corresponding treatment regimen.04-23-2009
20090104644GENETICALLY MODIFIED CYCLIC-NUCLEOTIDE CONTROLLED ION CHAN - A genetically modified cyclic-nucleotide controlled ion channels where the subunits thereof are altered in such a manner that they have a higher sensitivity for cAMP in relation to cGMP in comparison with the Wildtype according to Seq ID No. 1 and 2.04-23-2009
20090104641Electrochemical method for detecting hemoglobin or hematocrit and test strip thereof - The present invention provides an electrochemical method and a test strip for detecting hemoglobin in a specimen. The method includes the steps of providing the specimen with a reagent, which has a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by the reaction of the hemoglobin and the electron mediator in a specimen under a potentiostatic condition; and calculating a hemoglobin concentration in the specimen according to the detected electric current. In the method of the present invention, tetrathiafulvalene modified by cyclodextrin is used as an electron mediator, which directly interacts with the hemoglobin in the specimen. Since the hemoglobin concentration is in inverse proportion to the value of electric current or the value of SOC (state-of-charge) integrated by electric current value with time, the hemoglobin concentration in a whole blood specimen or the hematocrit level in a blood specimen is accurately and rapidly obtained according to the detected electric value or the value of SOC.04-23-2009
20090104640Method of Evaluating Biological Material and Bioreactor Therefor - A method of evaluating biological material comprising forming a 3-dimensional scaffold of tubular biological material, delivering a fluid through the tubular biological material, and evaluating the biological material and in particular evaluating the effect of biomolecules, medical devices and medical devices containing biomolecules on biological material. The invention further relates to a bioreactor suitable for evaluating biological material.04-23-2009
20090275071DIAGNOSTIC COMPOSITION AND ARTICLE FOR MONITORING INTRAVAGINAL INFECTIONS - The present invention provides a non-toxic diagnostic composition for intravaginal monitoring of vaginal infections. The present invention further provides a diagnostic article for intravaginal monitoring of vaginal infections with the article including an absorbent material for absorbing vaginal secretions and a composition suitable for identification of the pH or the buffer capacities associated with vaginal secretions. The diagnostic composition provides a visible indication of vaginal infections, such as bacterial vaginosis.11-05-2009
20090280522Method For Rapid Detection And Evaluation Of Cultured Cell Growth - Provided is a method and system for the rapid and accurate detection of growth and metabolism of a cellular microorganism in a population of microorganisms in a non-liquid, culture medium. Further provided is a gelled culture medium containing a non-toxic, water-soluble, phosphorescent compound which measures oxygen content (partial pressure) of a microorganism also contained therein, by oxygen-dependent quenching of phosphorescence; or the gel contains a fluorescent pH indicator that demonstrates growth of the microorganism by pH-dependent intensity change or wavelength shift in the emission spectrum. Further provided is a system and method for killing undesirable microorganisms or colonies in the culture medium without harming the surrounding microorganisms.11-12-2009
20090280517METHODS OF DIAGNOSIS AND PROGNOSIS FOR A MUSCULAR DYSTROPHY - The invention relates to the treatment, diagnosis, and prognosis of a muscular dystrophy or myopathy. The present inventors have found that the quantity of mu-crystallin is increased in a muscular dystrophy. In particular, the inventors have found that mu-crystallin is increased in facioscapulohumeral muscular dystrophy (FSHD). Based on the inventors' findings, the invention provides a novel means for the treatment, diagnosis, and prognosis of a muscular dystrophy or myopathy.11-12-2009
20090280518SYSTEM FOR HIGH THROUGHPUT MEASUREMENT OF MECHANICAL PROPERTIES OF CELLS - A system for measuring a mechanical property of a cell is provided. The system includes a body having a channel therethrough with a first end and a second end, the channel including at least one cell deforming feature configured to deform a cell passing through the channel. A first sensor system is positioned on the first end side of the cell deforming feature and a second sensor system is positioned on the second end side of the cell deforming feature, and the first and second sensor systems are configured to detect information about a cell as the cell travels across the cell deforming feature. A controller communicating with the first and second sensor systems is adapted to receive data from the first and second sensor systems and calculate a mechanical property of the cell.11-12-2009
20090286271Methods of Diagnosing and Treating Complications of Pregnancy - Disclosed herein are methods for treating a pregnancy related hypertensive disorder, such as pre-eclampsia and eclampsia, using combinations of compounds that alter soluble endoglin, endothelial nitric oxide synthase, PGI11-19-2009
20090286274METHOD OF MONITORING LIVE CELLS - The present invention relates to a method of monitoring live cells, comprising the sequential steps of: 11-19-2009
20100279334CYCLODIPEPTIDE SYNTHASES (CDSS) AND THEIR USE IN THE SYNTHESIS OF LINEAR DIPEPTIDES - Use of CDSs in the synthesis of linear dipeptides, and applications thereof for the in vivo and in vitro synthesis of linear dipeptides, in particular Phe-Leu, Leu-Phe, Phe-Phe, Phe-Tyr, Tyr-Phe, Leu-Leu, Leu-Tyr, Tyr-Leu, Phe-Met, Met-Phe, Leu-Met, Met-Leu, Tyr-Met, Met-Tyr, Met-Met, Tyr-Tyr, Ile-Met, Met-Ile, Leu-Ile, Ile-Leu using the corresponding polynucleotides.11-04-2010
20100279336METHOD FOR RAPIDLY MEASURING PREMATURE YEAST FLOCCULATING FACTOR OF MALT AND MEASURING APPARATUS THEREFOR - This invention provides a method for rapidly measuring an premature yeast flocculating factor of malt contained in a brewing material, comprising the following steps of: (1) mixing yeast in the late logarithmic growth phase or thereafter with a water-extracted high molecular fraction prepared from a test material sample in a buffer solution and suspending the mixture in the buffer solution; and (2) irradiating the suspension obtained in the step (1) with visible light, photographing the scattered light with a camera device, image-analyzing the image data thus obtained and determining the degree of whiteness of the suspension to measure the degree of sedimentation of the yeast in the suspension. According to the present invention, the premature yeast flocculating factor of malt contained in the brewing material can be measured in a highly accurate, rapid and simple manner, and, further, even a plurality of analytes can be rapidly measured.11-04-2010
20100136606METHOD OF MEASURING PHYSICAL PROPERTY VALUES OF CELL AND PHYSICAL PROPERTY MEASURING APPARATUS - Firstly, a shape of a cell as an object of a measurement is modeled, and a complex electric permittivity response when an AC electric field is applied to the cell is obtained through a numerical analysis (Step S06-03-2010
20090291466MULTINETWORK NERVE CELL ASSAY PLATFORM WITH PARALLEL RECORDING CAPABILITY - A neuronal network analysis plate having alternating rows of recording wells and amplifying wells. The recording wells contain a neural cell network and a series of electrodes for recording the action potential signals of the neurons. The electrodes are connected to amplifiers in adjacent amplifying wells. The close proximity of these amplifiers ideal because it permits the parallel, non-multiplexed recording of action potential signals from multiple different active nerve cell networks. The amplifiers in the amplifying wells can then be connected to external amplification equipment. The neuronal network analysis plate may be contained within a single commercially available 24 or 96 well plate. The neuronal network analysis plate can be used to detect and quantify pharmacological and toxicological responses of the neural cells to one or more agents in vitro.11-26-2009
20090291465Screening Method for Identifying New Drugs - A screening method for identifying new drugs A screening method for identifying a candidate to drug wherein said method 5 comprises the following steps: a) obtaining an expression vector which comprises a gene sequence codifying a naturally occurring pathogenic non-discriminating tRNA synthetase; b) transforming isolated mammalian cells with the expression vector; c) growing the recombinant cells resulting from (b) in a nutrient medium under conditions which allow the expression of the 10 pathogenic tRNA synthetase, resulting the expression of the pathogenic tRNA synthetase into cell death or a decrease in the rate of cell division; d) providing a substance to be tested; and e) analyzing the resulting cell growth, wherein if there is an increase in cell growth, then the substance selectively inhibits the activity of the pathogenic tRNA synthetase and does 15 not affect to its cellular ortholog, resulting that said substance is a candidate to drug.11-26-2009
20090298113EX VIVO HUMAN SKIN MODEL - The present invention relates to the use of an ex vivo human skin sample as a biological model, particularly for the assessment of pharmacological and cosmetic effects.12-03-2009
20100279332METHOD AND DEVICE FOR CHARACTERIZING BIOLOGICAL TISSUE - A method and a system for jointly determining the content of a first chromophorous and fluorescent compound and a second chromophorous compound in a biological tissue (11-04-2010
20100279337Biomarkers Related To Metabolic Age and Methods Using The Same - Biomarkers relating to metabolic age are provided, as well as methods for using such biomarkers as biomarkers for determining metabolic age. In addition, methods for modulating the metabolic age of a subject are also provided. Also provided are suites of small molecule entities as biomarkers for metabolic age.11-04-2010
20100279335APPARATUS FOR MEASURING BLOOD OXYGEN SATURATION - The invention relates to an apparatus for measuring blood oxygen saturation, comprising a housing (11-04-2010
20130217061Apparatus for Systematic Single Cell Tracking of Distinctive Cellular Events - An apparatus for quantitative identification of distinctive cellular events occurring in a cell population using a non-fluorescence approach. The apparatus comprises an image acquisition unit having a Differential Interference Contrast microscope with a camera, a light source, an environmental chamber allowing carrying out cell culture of at least one cell in a cell population; the image acquisition unit acquiring images of the cell population, at predetermined time points; a cell tracker for individually tracking the at least one cell of the cell population in the images; a distinctive cellular event detector for detecting an occurrence of a distinctive cellular event; a report generator; wherein the distinctive cellular event is selected from the group consisting of: tripolar, tetrapolar, quadpolar cell division, cell fusion, cell death, impaired cell division, cell shape alteration, nuclear shape alteration, inner cellular material accumulation, cell enlargement, engulfing, hyper-mobilization, hypo-mobilization and prolonged doubling time.08-22-2013
20110201043TRANSGENIC MAMMALS AND CELL LINES FOR THE IDENTIFICATION OF GLUTAMATE TRANSPORTER MODULATORS - The invention includes a transgenic, non-human mammal useful for identifying candidate compounds for treating neurological and/or psychiatric disorders. Incorporated into the genome of the transgenic mammal is a transgene comprising a glutamate transporter promoter operatively linked to a reporter gene. The transgenic, non-human mammal and cells isolated therefrom can be used as an in vivo model for the identification of candidate compounds useful for the treatment of neurological and/or psychiatric disorders.08-18-2011
20110201041ENCAPSULATION OF NANO-MATERIALS FOR FLUID PURIFICATION/SEPARATION - Disclosed is an apparatus and method whereby small particle nano materials may be contained in a highly functional package for fluid separation and/or purification applications. The package consists of an aerogel material which uniformly surrounds the nano-particles. The aerogel may be composed of carbon, silicon, or silicon oxide or other suitable materials. The morphological features of the aerogel may be tailored specifically towards fine particle and ultrafine particle containment while maintaining uniform fluid flow in separation and purification processes. The aerogel may be bonded to a suitable rigid housing by chemical or mechanical means.08-18-2011
20120295296Chlorite in the Treatment of Neurodegenerative Disease - The invention features methods of treating a macrophage-associated neurodegenerative disease such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), or multiple sclerosis (MS) in a subject by administering chlorite in an amount effective to decrease blood immune cell activation. The invention also features methods of monitoring therapy by assessing blood immune cell activation before and after therapy.11-22-2012
20120295297ASSAY METHOD USING ENCODED PARTICLE-BASED PLATFORM - Provided is an assay method using an encoded particle-based platform. In the assay method, first, a plurality of encoded particles having codes distinguishable from one another according to kinds of included target materials are prepared. The plurality of encoded particles are provided onto a plate including a plurality of wells by pipetting, and disposed in the plurality of wells by a self-assembly method. An analyte is provided into the plurality of wells. The codes of the plurality of encoded particles disposed in the plurality of wells are decoded. The target materials of the plurality of encoded particles are released to cause a reaction between the target materials and the analyte.11-22-2012
20120295298EX VIVO METHOD FOR DETERMINING POTENTIAL GLP-2 RECEPTOR MODULATORS - Disclosed herein is a method for measuring the contractility of intestinal tissue upon treatment with GLP-2 or a GLP-2 ligand. Also disclosed is an assay which directly measures the activity of GLP-2 or GLP-2 ligands ex vivo and permits the screening of putative GLP-2 ligands in native tissue.11-22-2012
20100136601GLASS-CELL VIAL FOR EXAMINING SLIGHT AMOUNT OF SPECIMEN - A glass-cell vial is provided which can examine specimen without causing any hindrance even when as minimum as possible specimen is used, and prevents mixture of foreign substances from causing examination errors.06-03-2010
20100285518PHOTOACOUSTIC DETECTION OF ANALYTES IN SOLID TISSUE AND DETECTION SYSTEM - A preferred system for detecting an analyte in solid tissue, such as an intact lymph node, in vitro includes a laser arranged to generate a pulsed laser beam into solid tissue, which can be a fully intact lymph node. An acoustic sensor, and preferably at least three acoustic sensors are arranged in different positions to span a three dimensional space, such as in an X, Y and Z coordinate system, to detect photoacoustic signals generated within the lymph node. At least one computer receives signals from the acoustic sensor(s). The computer determines the presence or absence of, and preferably the position of analyte, from the signals and the timing of the signals. A preferred method for detecting an analyte in a lymph node in vitro includes exposing an extracted lymph node to a pulsed laser beam. A photoacoustic signal is sensed. The photoacoustic signal is analyzed to confirm the presence or absence of an analyte in the lymph node. Preferably, multiple photoacoustic signals are sensed from sensors that span a three dimensional space and the position of analyte is also determined.11-11-2010
20090170148Nanog+, OCT-4+ Retinal Pigment Epithelial Stem Cells and Methods for Their Use and Manufacture - Retinal stem cells haying embryonic-like characteristics are disclosed. The retinal stem cells express one or more of the embryonic stem cell markers Nanog and OCT-4. The retinal stem cells may be obtained from retinal pigment epithelium. Methods of making and using retinal stem cells are also disclosed.07-02-2009
20090269800DEVICE AND METHOD FOR PROCESSING CELL SAMPLES - A cell analysis device and method is described which enables the user to efficiently treat cells in a sample. The cells can be treated with reagents at a time and place proximate to collection and may be handled in a automated or semi automated fashion.10-29-2009
20100143960CYANINE DERIVATIVES, FLUORESCENT CONJUGATES CONTAINING SAME AND USE THEREOF - A subject matter of the invention is cyanine derivatives of formula:06-10-2010
20100136596DEVICE FOR RECEIVING, TREATING, AND STORING SMALL VOLUME SAMPLES - A device is provided by means of which biomolecules in samples in a volume range from <1 μl to 500 μl can be received, treated and stored in a quick, reproducible and loss-free manner as easily, effortlessly and practically as possible, and without any risk of damaging the device. The device includes sample vessels that are configured, in terms of size and shape, as dimensionally and positionally stable capillaries that are open at both ends, the longitudinal walls of which are completely or partially made of a semi-permeable membrane.06-03-2010
20110201048SCREENING METHOD OF THERAPEUTIC AND DIAGNOSTIC AGENTS FOR TNF-ALPHA-INDUCED DISEASES USING REACTIVE OXYGEN SPECIES MODULATOR 1 - Disclosed are methods for screening therapeutics, prophylactics or pain alleviators for diseases induced by TNF-α, a main mediator of inflammation and in ROS production and cell death, and for diagnosing the diseases, and a diagnostic kit. For this, a nucleotide sequence and an amino acid sequence of Romo1, which plays an important role in the ROS production and cell death pathway of TNF-α signaling are utilized.08-18-2011
20110201047 NOVEL SORTING TECHNOLOGY THAT ALLOWS FOR HIGHLY EFFICIENT SELECTION OF SPERM WITHOUT CHROMATIN DAMAGE - This invention provides a method to select and enrich healthy sperm for use in assisted reproductive techniques comprising incubating sperm with at least one marking reagent that enters unhealthy sperm but not healthy sperm. The invention also provides a method to sort healthy sperm of high viability from apoptotic, necrotic and dead sperm by using YO-PRO-1 and PI for fluorescence activated cell sorting in flow cytometers. A kit comprising YO-PRO-1 and PI is also included in this invention.08-18-2011
20110201046T1R HETERO-OLIGOMERIC TASTE RECEPTORS - Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors arc described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for identifying putative taste modulating compounds using such hetero-oligomeric complexes also described, as is a novel surface expression facilitating peptide useful for targeting integral plasma membrane proteins to the surface of a cell.08-18-2011
20090004686Measurement and uses of oxidative stress - The present invention provides a method of determining the overall oxidative status of a body fluid or a tissue of a patient by measuring the oxidation-reduction potential (ORP) of the body fluid or tissue. The method has been found to be useful in the diagnosis, evaluation and monitoring of patients who have suffered a trauma (such as a head injury), patients suspected of being critically-ill, patients who have a viral infection, and patients suspected of having a myocardial infarction. The method has also been found useful in monitoring and evaluating exercise performance in patients. In addition, the method has been found useful in monitoring and evaluating stored blood products and patients who will receive such a product.01-01-2009
20080274494METHOD AND APPARATUS FOR CO2 SEQUESTRATION - A method and apparatus for growing algae for sequestering carbon dioxide and then harvesting the algae includes a container for a suspension of algae in a liquid and a bioreactor having a translucent channel in fluid communication with the container to absorb CO11-06-2008
20080274493APPARATUS AND METHODS FOR CONDUCTING ASSAYS AND HIGH THROUGHPUT SCREENING - The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.11-06-2008
20080274492Detection of platinum group metals with fluorophores via allylic oxidative insertion - A method of detecting platinum group metals in a sample is provided. The method comprises the step of contacting the sample with a fluorophore capable of undergoing allylic ether or allylic ester cleavage. The fluorophore has an oxygen-protected moiety, the protecting group having an allylic functionality. A reducing agent and optionally a solubilizer are also added to the sample. Very low levels of platinum group metals such as palladium and platinum can be detected in a sample.11-06-2008
20080274490PD-1, a receptor for B7-4, and uses therefor - Disclosed is a method for modulating an immune response by modulating signaling via PD-1. The immune response may be downregulated by increasing signaling via PD-1, or may be upregulated by decreasing signaling via PD-1. Agents which are useful for stimulating signaling via PD-1 to downregulate an immune response include an activating antibody that recognizes PD-1, a form of B7-4 that binds to an inhibitory receptor, and a small molecule that binds to PD-1. Agents which are useful for inhibiting signaling via PD-1 to upregulate an immune response include a blocking antibody that recognizes PD-1, a non-activating form of B7-4, an antibody that recognizes B7-4, and a soluble form of PD-1. Also disclosed is a method for modulating the interaction of B7-4 with an inhibitory receptor on an immune cell. The method comprises contacting an antigen presenting cell which expresses B7-4 with an agent such as a form of B7-4, a form of PD-1, or an agent that modulates the interaction of B7-4 and PD-1. Also disclosed is a method for inhibiting activation of an immune cell via a non-apoptotic mechanism by increasing the activity or expression of PD-1 in the immune cell. The disclosed methods for modulating an immune response are useful, for instance as therapeutic treatment of a subject with conditions that would benefit from immune response modulation. For instance, a condition such as a tumor, a neurological disease, or an immunosuppressive disease, would be treated by upregulating an immune response, and conditions such as a transplant, an allergy, or an autoimmune disorder would benefit from downregulation of an immune response. Assays for identifying compounds which modulate the activity of, or signaling via, B7-4, or PD-1, especially which modulate the binding of B7-4 or PD-1 to a target molecule are also disclosed. Further disclosed are a vaccine and also other compositions which contain agents that modulate signaling via PD-1.11-06-2008
20080280319GSH adducts and uses thereof - This invention relates to biomarkers of oxidative stress and their use. Specifically, the invention relates to thiadiazabicyclo-4-oxo-2(E)-nonenal-Glutathione adduct as a novel biomarker of oxidative stress and its diagnostic use.11-13-2008
20080286828MATERIALS AND METHODS RELATING TO G-PROTEIN COUPLED RECEPTOR OLIGOMERS - The invention provides materials and methods relating to G-protein coupled receptor (GPCR) oligomers. Complexes of two or more GPCRs associated with G-proteins are provided. Also provided are fusion proteins comprising a GPCR and a G-protein, nucleic acids, expression vectors and host cells. Methods of producing the complexes and fusion proteins of the invention are also provided.11-20-2008
20080286826Luminescent Particle and Method of Detecting a Biological Entity Using a Luminescent Particle - The invention provides a luminescent particle (11-20-2008
20080286824System for Screening Cells for High Expression of a Protein of Interest (Poi) - This invention refers to industrial production of proteins. More particularly, the invention refers to a fusion protein as a novel chimeric selection marker comprising a peptide conferring resistance to an antibiotic, or a fragment, allelic variant, splice variant or mutein thereof, and at least one sequence comprising SEQ ID NO: 1, 2 or 3, preferably for producing a protein of interest (POI). The inventive chimeric selection marker exhibits: (i) a resistance to an antibiotic; and (ii) a fluorescence activity upon binding of a ligand to the sequence comprising SEQ ID NO: 1, 2 or 3. The invention further refers to nucleic acids encoding the inventive fusion protein and to expression vectors, comprising the inventive fusion protein and additionally the protein of interest (POI). Finally, uses of the inventive chimeric selection marker for screening cells for high expression of a protein of interest (POI) are disclosed.11-20-2008
20080286823Apparatus and method to measure platelet contractility - An apparatus and method for measuring blood platelet contractility, hereinafter called a “retractometer” is disclosed. Also disclosed is a system apparatus and method for automatically measuring platelet contractility in a plurality of samples, having an array of retractometer units and an electronic solenoid valve controller to fully automate screening in clinical and research situations and save costs in labor.11-20-2008
20080286822Systems and methods for detecting toxins in a sample - In one aspect the invention provides a system for detecting the presence of toxins in a sample that includes a plurality of chambers for culturing organisms and observing the organism's motility response when introduced into a sample containing a toxin. The toxicity measurement system may include an imaging module to monitor and track the movement of one or more organisms in the sample and identify abnormalities. In other aspects, the invention provides methods of culturing organisms and detecting the presence of toxins in the sample using the motility response of organisms in the sample.11-20-2008
20080286821Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.11-20-2008
20110269166SEALING SURFACE SAMPLING PROBE APPLIED TO BLOOD SPOTS AND TISSUES - A sample assembly includes a stack of a sample and a hydrophobic elastic layer located on the back side surface thereof or a filter layer. The sample may include a biological material or a chemical, and can include an absorptive material. The filter layer includes an absorptive material in which a sample material is embedded. A sealing surface sampling probe including a knife edge lands on an area of interest in the sample or the filter layer. The hydrophobic elastic layer or the substrate in conjunction with the knife edge provides a complete sealing of a confined portion of the sample or the filter layer to prevent inclusion of any other material from outside the confined volume and to enhance the precision and sensitivity of the analysis.11-03-2011
20080293087SULFONYLUREA RECEPTOR SHORT FORMS FROM MITOCHONDRIA AND USES THEREOF - The present invention relates to isolated sulfonylurea receptor polynucleotides and polypeptides, as well as vectors and cells lines containing the polynucleotides and polypeptides. The present invention also relates to methods of using cell lines containing the polynucleotides and polypeptides to identify agents that are useful in ischemic preconditioning.11-27-2008
20080293086REAL TIME MONITORING OF MICROBIAL ENZYMATIC PATHWAYS - This invention provides compositions and methods for monitoring and regulating the production of a target product of a biochemical pathway in an organism, such as butanol. A gene encoding a light-emitting reporter molecule, such as luciferase, is operatively linked with a transcription regulatory nucleotide sequence that regulates transcription of an enzyme in the pathway that signals the rate of production of the target product, such as butanol dehyrogenase. When a microorganism is transfected with such a reporter construct and cultured, the reporter is expressed contemporaneously with the enzyme. The amount of light produced by the reporter indicates amount of enzyme being produced which, in turn, signals the amount of target product being produced. When the reporter is measured in real time, it provides information that can be used to regulate culture conditions and to optimize production of the target product.11-27-2008
20080293092CALCIUM CHANNEL PROTEINS AND USES THEREOF - Described herein are compositions and uses thereof related to Ca11-27-2008
20080293089Assays for Modulators of Parkin Activity Using Novel Substrates - The invention provides in vitro and cell-based assays for parkin activity, in which parkin-mediated ubiquitination of the S5a subunit of the 26S proteasome is measured, or ubiquitination of troponin 1. The assays may be used to screen for agents that modulate parkin protein ligase activity.11-27-2008
20090269799METHOD OF DETERMINING A COMPLETE BLOOD COUNT AND A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.10-29-2009
20100099134METHODS FOR DETECTING SULFHYDRYL-CONTAINING COMPOUNDS IN A BIOLOGICAL TEST SAMPLE - The invention relates to methods for detecting the presence of a compound of formula I in a biological test sample:04-22-2010
20090269793Compounds and Kits for the Detection and the Quantification of Cell Apoptosis - The present invention relates a compound of formula (1) wherein: —R1 represents a linear alkyl group of 4 to 20 carbon atoms; —Y represents a linear alkyl group of 1 to 5 carbon atoms or a group of formula —R—O—R′—, —R—CO—R′— or —R—CO—NH—R′—, in which R represents a linear alkyl group of 1 to 3 carbon atoms, R′ represents a linear alkylene group of 0-3 carbon atoms, Y being linked to the bicycle in position 6 or 7; -Z represents a linear alkyl chain of 3 or 4 carbon atoms; -A represents an oxygen atom, a sulphur atom, or a —NH group, or an amminoalkyl group —NR″ in which R″ represents an alkyl group of 1 to 20 carbon atoms; —Ar represents an aromatic cycle or polycycle consisting of 6 to 14 carbon atoms, or an aromatic heterocycle, said heterocycle containing 4, 5 or 6 carbon atoms and at least one heteroatom selected in the group consisting of N, S, and O, or a condensed aromatic heterobicycle, said heterobicycle consisting of 6 to 9 carbon atoms and at least one heteroatom selected in the group consisting of N, S, and O; —R2 and R3, which are identical or different, each representing a hydrogen atom or an alkyl group of 1 to 4 carbon atoms, R2 and R3 optionally forming a 5- to 7-membered ring with the nitrogen atom; —R4, R5 and R6, identical or different, represent an hydrogen, a linear alkyl group or a linear oxyalkyl group of 1 to 4 carbon atoms.10-29-2009
20080318265Measuring the Thickness of Organic Samples - This invention relates to a quality control method for improving histoprocessing, as well as a device for measuring the thickness of an organic tissue sample suited for use in the method.12-25-2008
20100143963Modular Droplet Actuator Drive - The invention provides a droplet actuator drive. In certain embodiments, the droplet actuator drive may include a detection apparatus for sensing a property of a droplet on a droplet actuator; circuitry for controlling the detection apparatus electronically coupled to the detection apparatus; a droplet actuator cartridge connector; circuitry for controlling a droplet actuator coupled to the droplet actuator connector; and/or a means for coupling the droplet actuator circuitry to a processor. Systems, kits and methods of conducting assays are also provided.06-10-2010
20090004685Interface Device and Method for Using the Same - The present disclosure is related to an interface device for providing access to a network to be monitored. The interface device includes a plurality of elements, the elements being sensors and/or actuators. A selection means is provided for selecting a subset of elements among the plurality of elements, each element of the subset being arranged for outputting and/or receiving a signal. A local memory is provided for storing the subset.01-01-2009
20110207163METHOD FOR AND MATERIAL OF A SHAPE STANDARD - A method is disclosed for the preparation of isomorphic and nonspherical shape standards as isometric as possible and their mixtures for validation of the measurement methods and analyzers for quantification of particle shapes and their characteristics especially in the microscale and nanoscale size range. Furthermore the shape standards also provide quality control and performance qualification can be used at the operator of the corresponding shape measurement devices.08-25-2011
20110269174REGULATORS OF NFAT - Disclosed are methods of identifying an agent that modulates an NFAT regulator protein. One such method comprises contacting at least one test agent with a recombinant cell comprising at least one NFAT regulator protein or fragment or derivative thereof, assessing the effect of the test agent on an activity, interaction, expression, or binding to the NFAT regulator protein or fragment or derivative thereof, and identifying the test agent that has an effect on an activity, interaction, expression, or binding to the NFAT regulator protein or fragment or derivative thereof, whereby the identified test agent is characterized as an agent that modulates an NFAT regulator protein. Methods of identifying an agent that modulates intracellular calcium, methods to screen for an agent that modulates NFAT regulator function, methods to diagnose unexplained immunodeficiency in a subject, and methods for identifying an agent for treating or preventing a disease or disorder associated with a NFAT regulator protein or calcium signaling are also disclosed.11-03-2011
20110269170pH Sensitive Metal Nanoparticle and Preparation Method - The present invention relates to a pH sensitive particle, a method of preparation thereof, and a use thereof. More particularly, the invention provides a pH sensitive metal nanoparticle and its use for medical treatment utilizing cell necrosis during photothermal therapy. The pH sensitive metal nanoparticle based on this invention consists of a pH sensitive ligand compound whose charge changes depending on the pH of the metal nanoparticle. The particle can be collected in cells, such as cancer cells which present an abnormal pH environment. The pH sensitive metal nanoparticle based on this invention can induce cell death through a photothermal procedure after aggregation. Therefore, the invention enables medical treatment using cell necrosis for e.g. cancer treatment.11-03-2011
20100273202CODON-OPTIMIZED NUCLEIC ACID FOR CODING APO-CLYTIN-II AND METHOD FOR USING THE SAME - A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.10-28-2010
20120196311Using cells reprogrammed with oncogenic factors for screening anti-neoplastic agents - Cells reprogrammed with oncogenic factors (CROFs) include incorrectly programmed stem cells such as induced pluripotent stem cells (iPSCs). The present application discloses linkages between iPS reprogramming and its potential roles in neoplastic transformation and thus establishes a foundation for using iPSCs as a class of CROFs for screening anti-neoplastic agents. The screening process involves examining the capability of a single agent or a combination of multiple agents in suppressing a neoplastic process including aerobic glycolysis and the related anabolism and thus inhibiting excessive reproduction of the neoplastic cell. In addition, agents are also screened for their potential in inhibiting invasion and migration of neoplastic cells. Anti-neoplastic agents found through methods disclosed here may represent wide-spectrum anti-neoplastic agents but possess very limited side effect because they target at one or more unique aspects of neoplastic processes, rather than affecting one or more living processes common to all cells including normal cells.08-02-2012
20110269165LACTIC ACID BACTERIA AND THEIR CELLULAR COMPONENTS INDUCING IMMUNOREGULATORY FUNCTION, AND METHOD OF OBTAINING THE SAME - To provide a microorganism and an ingredient thereof that contribute to prevention and treatment of immune diseases including allergy, autoimmune diseases and inflammatory bowel diseases (e.g., large-intestinal ulcer), a method of effectively selecting the microorganism, and a method of efficiently inducing immunoregulatory cells that play an important role on maintaining immunological homeostasis using the microorganism or the ingredient thereof. The present invention provides a 11-03-2011
20110269167MEDICAL APPARATUS FOR EXTRACORPOREAL BLOOD TREATMENT AND METHOD FOR DETERMINING A BLOOD PARAMETER VALUE IN A MEDICAL APPARATUS THEREOF - A medical apparatus for extracorporeal blood treatment and a method for determining blood parameter value have been provided. The medical apparatus has a control unit (11-03-2011
20100273201Acetolactate Synthase (ALS) Selectable Marker from Trichoderma reesei - A nucleic acid encoding an acetolactate synthase (ALS) protein that provides resistance to ALS inhibitors, e.g., sulphonylurea and imidazolinone compounds, is provided. The nucleic acid may be used as a selectable marker for expression of a protein of interest in host cells.10-28-2010
20090181420Inhibiting Smad signaling promotes neuron regeneration - Regeneration of a lesioned CNS axon of a mature neuron, determined to be subject to regeneration inhibition by Smad2/3 signaling, is promoted by contacting the neuron with an inhibitor of Smad2/3 signaling sufficient to promote regeneration of the axon.07-16-2009
20110207162Dynamic visualization of expressed gene networks in living cells - The present invention provides functional annotation of novel genes by detection of interactions of their encoded proteins with known proteins followed by assays to validate that the gene participates in a specific cellular function. The instant invention also provides an experimental strategy that allows for detection of protein interactions and functional assays with a single reporter system. Interactions among network component proteins are detected and probed with stimulators and inhibitors of the network and subcellular location of the interacting proteins is determined. Additionally, applicants' use this strategy to map a signal transduction network that controls the G08-25-2011
20110207164Method for Assessment of Folate Phenotypes, Disease Risk and Response to Therapy - The invention provides a method for measuring the levels of 5-methyltetrahydrofolate (5-MTHF), tetrahydrofolate (THF), and 5,10-MTHF in a biological sample. The method includes employing an isotope dilution liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) methodology.08-25-2011
20110033885METHOD FOR MANUFACTURING A TISSUE-ENGINEERED CONSTRUCT - Method for manufacturing a tissue-engineered construct, such as a heart valve, comprising the steps of providing a-cell-seeded scaffold in a bioreactor chamber which bioreactor chamber is divided by the cell-seeded scaffold into a first compartment and a second compartment, subjecting the cell-seeded scaffold to a flow of nutrient medium within the bioreactor chamber for developing the cell-seeded scaffold to a tissue structure and next to the tissue construct, applying a dynamic pressure difference to the developing tissue structure by the flow of nutrient medium to induce dynamic strain on the tissue structure.02-10-2011
20110269175METHODS FOR SORTING PARTICLES - A method of sorting stained particles in a flow cytometer by generating a mathematical model based upon extracted features of waveform pulses produced in response to the stained particles. Waveform pulses can then be analyzed to determine the probability the stained particles belong in either a first or a second population and a decision boundary can be established. Sorting decisions can be made based upon the extracted features of the waveform pulses relative to the decision boundary in combination with other desirability factors for particles or droplets containing the particles.11-03-2011
20110269171METHODS AND MATERIALS FOR USING NKAP POLYPEPTIDE EXPRESSION LEVELS OR PATTERNS TO DETECT OR ASSESS CANCER - This document relates to methods and materials involved in detecting or assessing cancer (e.g., ovarian cancer). For example, methods and materials involved in using NKAP polypeptide expression levels and patterns to diagnose cancer, assess cancer survival, and assess cancer treatment options are provided.11-03-2011
20090298114APPARATUS AND METHODS FOR IMAGING AND MODIFICATION OF BIOLOGICAL SAMPLES - This invention relates to enrichment of a biological sample comprising a plurality of cells to assist further analysis thereof. It provides a technique comprising the steps of: (a) providing a sample comprising a plurality of cells which include a photosensitive compound that can be induced by light irradiation to inactivate or kill at least part of the respective cell; (b) acquiring an image of at least a portion of the sample; (c) identifying cells of interest in the sample image; (d) selecting cells other than the cells identified in step (c); and (e) irradiating only those cells selected in step (d) with a light beam so as induce the photosensitive compound therein to inactivate or kill at least part of those cells, and thereby enrich the sample with respect to the cells of interest for further analysis.12-03-2009
20090298112MOUSE MODEL AND TREATMENT OF HEREDITARY INCLUSION BODY MYOPATHY - Disclosed herein are methods of treating HIBM in a subject comprising identifying subject in need thereof, and administering to the subject a compound, or a pharmaceutically acceptable salt, ester, amide, glycol, peptidyl, or prodrug thereof, wherein the compound is a compound that is biosynthesized in a wild type individual along a biochemical pathway between glucose and sialic acid, inclusive. Also disclosed herein are vectors comprising a nucleic acid sequence that encodes a polypeptide having at least 80% sequence identity to the sequence set forth in SEQ ID NO:2, recombinant cells comprising these vectors, and recombinant animals comprising the cells. In addition, methods of identifying a compound having therapeutic effect for HIBM are disclosed.12-03-2009
20090162887Particle analysis in an acoustic cytometer - The present invention is a method and apparatus for acoustically manipulating one or more particles.06-25-2009
20090162885Systems and methods for measuring fluid properties - A fluid property measurement system for measuring free stream particulates comprises a fluid movement device positioned within a fluid container which is configured to cause fluid flow within the fluid container along a fluid flow path when a fluid is present. A constricted region along the fluid flow path generates a region of concentrated streamlined flow within the constricted region and mixing of the fluid outside of the constricted region. A property measuring device is positioned with respect to the constricted region to measure fluid properties in the region of streamlined flow.06-25-2009
20090130699METHOD FOR TESTING SUBSTANCES ON BIOMATRICES - The invention relates to a method for testing one or several substances. According to said method, a tissue equivalent is cultivated, the substance/s is/are made to affect the tissue equivalent, and it is determined whether the effect of the substance/s has resulted in a modification of the tissue equivalent and/or the substance/s. The tissue equivalent comprises at least one cell and a porous matrix based on a biologically compatible polymer or polymer mixture. The matrix is provided with pores having a maximum size of 150 μm as well as pores having a minimum size of 300 μm while the degree of porosity is 93 to 98 percent.05-21-2009
20090136987Carbon Nanotube Based Imaging Agents - Compositions and methods related to carbon Nanotubes are provided. More particularly, imaging agents comprising carbon Nanotubes internally loaded with a contrast agent and associated methods are provided. One example of a method may involve a method for imaging comprising: providing an imaging agent comprising a carbon Nanotube loaded with contrast agent; introducing the imaging agent into a cell; and imaging the cell to detect the presence of the imaging agent.05-28-2009
20090136988METHOD AND DEVICE TO FRACTIONATE STEM CELLS - A method and the relevant instrumentation to fractionate living, adherent stem cells, particularly of human origin, from different sources is disclosed, said method is based on the non-equilibrium, dynamic fractionation of cells assisted by the Earth gravity field.05-28-2009
20090136986METHODS AND CELLS FOR CREATING FUNCTIONAL DIVERSITY AND USES THEREOF - The invention provides methods and compositions relating to cells having altered functions, and the nucleic acids that impart those functions. Altered cellular function arises from in vivo directed recombination of genetic elements to yield a recombined nucleic acid. These methods and compositions may utilize altered host cells having altered recombination enzyme profiles and/or altered recombination sites. The invention involves in some aspects methods for assembling nucleic acid molecules, such as genomic DNA. Aspects of the invention also provide kits, compositions, devices, and systems for generating novel recombined nucleic acids and cells having altered cell function.05-28-2009
20090136985Vascular Aging-Predicting Factor and Utilization of the Same - Abstract A predicting factor for vascular aging and an examination method for an early-stage lesion resulting from vascular aging are provided. Specifically, a predicting factor for vascular aging comprising a human apolipoprotein B100 having a glutathionylated thiol group; a method of examining a lesion resulting from vascular aging contain measurement of a human apolipoprotein B100 having a glutathionylated thiol group in the blood sample; an antibody specifically recognizing an apolipoprotein B100 having a glutathionylated thiol group; a diagnostic agent or a diagnostic kit for an early-stage lesion resulting from vascular aging containing an antibody recognizing a human apolipoprotein B100 having a glutathionylated thiol group, are provided.05-28-2009
20090136984METHOD AND MEANS FOR ENRICHMENT REMOVAL AND DETECTION OF LISTERIA - The present invention relates to polypeptide fragments of endolysin Ply511, which recognise and bind 05-28-2009
20090136983TARGET SEQUENCES FOR SYNTHETIC MOLECULES - The invention is based on the discovery that certain biarsenical molecules react with specified target sequences, thereby providing a facile means for labeling polypeptides containing the target sequence. The invention is useful in creating stable mammalian cell lines expressing a certain tetracysteine tagged polypeptides, thereby overcoming toxicity associated with native tetracysteine. In addition, the invention allows for orthogonal labeling of polypeptides, thereby allowing for the observation of protein-protein interactions and conformational changes in proteins, for example.05-28-2009
20090136982CELL SEPARATION USING MICROCHANNEL HAVING PATTERNED POSTS - A micro now device (05-28-2009
20090186375Method for Determining Bioactivity of Molecules - The present invention relates to a method for determining activity of a molecule, wherein the molecule comprises at least two active parts, and wherein the first part is capable of modulating the activity of cells, and the second part can specifically bind to a target, and kits useful for performing the method.07-23-2009
20090186376sVEGFR-2 AND ITS ROLE IN LYMPHANGIOGENESIS MODULATION - Disclosed herein are nucleic acid molecules comprising a nucleotide sequence of sVEGF-2, proteins encoded by those sequences and antibodies that bind to the protein. Also disclosed are methods for inhibiting or enhancing expression or activity of sVEGFR-2 and methods for inhibiting graft rejection, particularly cornea graph rejection. Also described are methods for inhibiting lymphangiogenesis and lymphatic endothelial cell proliferation by administering an effective amount of sVEGFR-2 and methods for treating lymphedema by inhibiting the activity of sVEGFR-2.07-23-2009
20090186373Pyrimidines reacting with O6-Alkylguanine-DNA alkyltransferase - The invention relates to pyrimidines suitable as substrates for O07-23-2009
20090186374APPARATUS FOR MEASURING EFFECT OF TEST COMPOUNDS ON BIOLOGICAL OBJECTS - An apparatus and method for real-time measurement of an effect of different concentrations of a test compound or series of test compounds on living cells, in which a flow of cell suspension is combined with a flow of the test compound and a cellular response of the living cells is repeatedly measured by a detector along a length of a detection zone where the cell suspension-test compound mixture is situated. The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test compounds and generation of three-dimensional dose/response/time profiles within a short timespan.07-23-2009
20090325217METHOD AND APPARATUS FOR SORTING CELLS - A method, apparatus, and system for a sorting flow cytometer include an objective lens having an optical axis coaxially aligned with the flow path at the focal point. A controllable energy source selectively alters an analyte according to a determination of whether the analyte is in a desired sub-population. In various embodiments, one or both of the emission from the controllable energy source and/or the emission from an illumination energy source passes through the objective lens. In some embodiments in which the emission from the controllable energy source passes through the objective lens, the objective lens may focus the emission from the controllable energy source at a different point than the focal point of a signal detected from the analyte and, in particular, at a point closer to the objective lens.12-31-2009
20090325211System and method for dual-detection of a cellular response - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor.12-31-2009
20090325216Process for the Preparation of Multicellular Spheroids - The invention pertains to a process for the preparation of multicellular spheroids from a suspension of single cells, wherein the cells are directly derived from a biological tissue and/or from cell-containing bodily fluid. The invention is further directed to the multicellular spheroids obtained by the process according to the invention as well as to the use of the spheroids for diagnostic, screening and therapeutic purposes.12-31-2009
20090325215CELLOMICS SYSTEM - In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.12-31-2009
20090325210Counting Bacteria and Determining Their Susceptibility to Antibiotics - A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided.12-31-2009
20090023177ASSAYS AND METHODS FOR DETERMINING STIM2 ACTIVITY - The present invention provides assays and methods for determining levels of STIM2 activity, thus providing tools for the characterization and study of the regulation of intracellular calcium levels.01-22-2009
20090023175Device for sampling a fluid and detecting an analyte therein - Provided is a device for detecting an analyte in a fluid comprising a first compartment (01-22-2009
20130217062METHOD FOR EVALUATING THE IMPROVEMENT OF SKIN ELASTICITY USING A MIMETIC DERMIS - The present invention relates to a method for evaluating the improvement of skin elasticity by preparing a mimetic dermis having a structure similar to that of the real dermis, and providing a three-dimensional visualization of the differences in the degree of transformation of the mimetic dermis.08-22-2013
20090081719BIOCHEMICAL MARKERS FOR ACUTE PULMONARY EMBOLISM - The present invention relates to a method of differentiating between a singular and a multiple lung embolism in a subject suspected to suffer from acute lung embolism comprising determining the amount of NT-proBNP in a sample of a subject suspected to suffer from acute lung embolism and comparing the amount to a reference amount. Further, the present invention also relates to a method of differentiating between acute and chronic lung embolism in a subject comprising determining the amount of NT-proANP at a first and a second time point and comparing the determined amounts with each other. The present invention also encompasses devices and kits for carrying out the aforementioned methods.03-26-2009
20110143387HIGHLY WATER-SOLUBLE, CATIONIC LUMINESCENT LABELS - Luminescent labels based on aromatic and heterocyclic compounds, including reactive intermediates used to synthesize these compounds, and methods of synthesizing and using these reporter compounds. These labels combine high photostabilities, large Stokes' shifts and contain a pyrimidinium moiety as a water-soluble group. These luminescent compounds relate generally to the following structure:06-16-2011
20110229927SAMPLE PORT OF A CELL CULTURE SYSTEM - Disclosed herein is a cell culturing system comprising a culturing chamber for culturing a biological cell in a growth medium and a sensor for measuring a signal in the spent growth medium, wherein the culturing chamber is provided in a mesoscale bioreactor platform with an inlet opening for an influent stream of growth medium and a outlet opening for an effluent stream of spent growth medium, said outlet opening, said spent growth medium being in fluid communication with a sample port for releasable adoption of the sensor. Furthermore, a method of measuring an effluent stream of spent growth medium from a culturing chamber in the cell culturing system is disclosed.09-22-2011
20090053755PROBE BASED MOLECULAR SIGNAL DELIVERY FOR PRECISE CONTROL AND MEASUREMENT OF SINGLE CELL RESPONSES - A system for producing and visualizing the response of a cell. The cell has a surface. A probe is provided. The probe is derivitized so that at least one bio-active molecule is covalently linked to the probe. The probe with the at least one bio-active molecule covalently linked to the probe is positioning over the surface of the cell. The at least one bio-active molecule is delivered to the surface of the cell producing a response of the cell. The response of the cell to the at least one bio-active molecule is visualized.02-26-2009
20090053751Chemiluminescent Method and Device for Evaluating the In Vivo Functional State of Phagocytes - A method of assessing the in vivo state of phagocytes in a patient, possibly indicating diagnostically important states such as inflammation or infection, which method utilizes chemiluminescent (CL) light emitted during the reaction in vitro between a CL substrate and the reactive oxygen species (ROS) formed in a fluid sample obtained from the patient. The measurement is performed in two or more portions of the sample, with stimulated phagocytes affected by one or more priming agents which shift the functional state of the phagocytes, providing a plurality of measurements, which are analyzed so as to distinguish intracellular and extracellular contributions to the CL kinetics. The results are compared with a range of control measurements performed with patients suffering from various diagnostic conditions.02-26-2009
20090053750Methods and compositions for identifying a cellular immune response against prostate cancer - The present invention relates to filamin-B peptides, compositions comprising such peptides, and methods of using such peptides to assess an immune response against such peptides. An immune response against the peptides correlates with an immune response, in particular a cellular immune response, against prostate cancer cells which immune response is preferably associated with prophylaxis of prostate cancer, treatment of prostate cancer, and/or amelioration of at least one symptom associated with prostate cancer.02-26-2009
20090053749Method and Apparatus for High Throughput Diagnosis of Diseased Cells With Microchannel Devices - The method and apparatus of the present invention detects changes in cell biomechanics caused by any of a variety of diseases and conditions. In one embodiment, the method and apparatus of the invention detect infection of red blood cells. In one embodiment, the invention is a method and apparatus comprising a microfluidic channel with a constriction, for trapping infected red blood cells while allowing healthy red blood cells to deform and pass through the channel. In another embodiment, the invention comprises a suspended microchannel resonator for detecting and counting red blood cells at the constriction of the microfluidic channel.02-26-2009
20090053753FUNCTIONAL IDENTIFICATION OF PROTEINS UNDERLYING Icrac ACTIVITY IN A CELL - The invention provides methods and compositions for determining the identity of CRACM homologs underlying Icrac activity in cells.02-26-2009
20090142789Surface Preparation Method - A method of covalently immobilising a charged chemical species on or near a sensor surface of a mass-sensitive chemical sensor, the sensor surface bearing functional groups capable of forming covalent bonds with the chemical species, the method involving the application of an electric field between the charged chemical species and the sensor surface such that the electrostatic attraction therebetween is increased.06-04-2009
20080286829APPARATUS AND METHOD FOR DETECTING LIVE CELLS WITH AN INTEGRATED FILTER AND GROWTH DETECTION DEVICE - A device for rapid concentration and detection of live cells in fluids includes a filter to capture a cell sample. The filter includes a first physical barrier with apertures of a first size and a second physical barrier with apertures of a second size smaller than the first size to isolate the cell sample on the filter. Growth detection circuitry associated with the filter electrically measures a cell growth rate associated with the cell sample in less than 2 days. The growth detection circuitry includes a mechanical filter for concentration of cells. The filter and growth detection circuitry are integrally formed within the device, which is sealed.11-20-2008
20090117607Methods For Differential Detection Of Hypoxic Tissue From A Tissue Sample Of A Mammalian Subject - Methods are provided for detecting levels of low oxygen in a tissue of a mammalian subject by administering non-polar halogen compounds to the mammalian subject. Detection may be accomplished using imaging methods involving fluorescence immunohistochemical imaging of the halogen compound.05-07-2009
20090117605Software integrated cytometric assay for quantification of the human polymorphonuclear leukocyte fctri receptor (CD64) - The invention relates a method of quantifying CD64 and CD163 expression in leukocytes and, specifically to a kit for use with a flow cytometer including a suspension of quantitative fluorescent microbead standards, fluorescent labeled antibodies directed to CD64 and CD163, and analytical software. The software is used to take information on the microbead suspension and fluorescent labeled antibodies from a flow cytometer and analyse data, smooth curves, calculate new parameters, provide quality control measures and notify of expiration of the assay system.05-07-2009
20090221019Core-Modified Terpene Trilactones From Ginkgo Biloba Extract and Biological Evaluation Thereof - Lactone-rings of ginkgolides are converted into the corresponding tetrahydrofuran moieties via DIBAL-H reduction followed by deoxygenation of the formed lactols with Et09-03-2009
20090197292SYSTEMS AND METHODS FOR EX VIVO LUNG CARE - Methods and systems of maintaining, evaluating, and providing therapy to a lung ex vivo. The methods and systems involve positioning the lung in an ex vivo perfusion circuit; circulating a perfusion fluid through the lung, the fluid entering the lung through a pulmonary artery interface and leaving the lung through a left atrial interface; and ventilating the lung by flowing a ventilation gas through a tracheal interface. Maintaining the lung for extended periods involves causing the lung to rebreath a captive volume of air, and reaching an equilibrium state between the perfusion fluid and the ventilation gas. Evaluating the gas exchange capability of the lung involves deoxygenating the perfusion fluid and measuring a time taken to reoxygenate the perfusion fluid by ventilating the lung with an oxygenation gas.08-06-2009
20090197294MRD-BASED REACTORS - The present invention depicts an MRD-based reactor. The MRD-based reactor comprises of a means for containing a flowing media and reacting the same (reactor). The reactor is characterized by a continuous wall portion, and is in connection with at least one MRD, adapted for applying localized spectroscopy towards the media. MRD-based reactors, in which the MRD is at least partially inside the reactor or reaction media, and those in which the MRD accommodates the reactor, are also introduced. Lastly, the invention teaches an in situ method for controlling and analyzing of a reaction. The method comprises of providing an MRD-based reactor; applying a magnetic field within the reactor, especially for performing a plurality of localized spectroscopic measurements and either real time or offline analyzing and/or controlling of reactions in the flowing media.08-06-2009
20120142045USE OF AN AMORPHOUS SILICON LAYER AND ANALYSIS METHODS - A method of detecting substances or reactions of substances in a sample, comprising: (i) providing a layer (CS) based on hydrogenated or unhydrogenated amorphous silicon with attached probes, (ii) bringing the layer (CS) in contact with the sample that may contain the substances that bind specifically to or reacts specifically with the probes, under appropriate conditions for the substances to bind to or react with the probes; (iii) optionally removing non-specifically bound or non-specifically reactive substances; and (iv) detecting the presence or amount of the specifically bound or reactive substances in the sample by surface plasmon resonance (SPR) and/or fluorescence, is described.06-07-2012
20090208997GPR18 as a Biomaker for TH1 Mediated Immune Response - The present invention provides the use of GPR18 in diseases or disorders mediated by Th1 cell activation.08-20-2009
20120171713SINGLE CHIP HAVING THE CHEMICAL SENSOR AND ELECTRONICS ON THE SAME DIE - A semiconductor die includes a chemical sensor, a digital to analog converter, and microcontroller formed therein. The chemical sensor detects the presence of a chemical and outputs an analog signal to the digital to analog converter. The analog to digital converter converts the analog signal to a digital signal. The analog to digital converter outputs the digital signal to the microcontroller. Microcontroller calculates a value of the concentration of the selected chemical.07-05-2012
20080261261System/unit and method employing a plurality of magnetoelastic sensor elements for automatically quantifying parameters of whole blood and platelet-rich plasma - A system/analyzer-unit and method/platform—using information obtained from at least one, adapted for a plurality of, magnetoelastic sensor elements in contact with one or more samples comprising blood from a patient—for automatically quantifying one or more parameters of the patient's blood. Information obtained from emissions measured from each of the sensor elements is uniquely processed to determine a quantification about the patient's blood, such as, quantifying platelet aggregation to determine platelet contribution toward clot formation; quantifying fibrin network contribution toward clot formation; quantifying platelet-fibrin clot interactions; quantifying kinetics of thrombin clot generation; quantifying platelet-fibrin clot strength; and so on. Structural aspects of the analyzer-unit include: a cartridge having at least one bay within which a sensor element is positioned; each bay in fluid communication with both (a) an entry port for injecting a first blood sample composed of blood taken from the patient (human or other mammal), and (b) a gas vent through which air displaced by injecting the first blood sample into the bay.10-23-2008
20080261259Culture Media For Expansion and Differentiation of Epidermal Cells and Uses Thereof For In Vitro Growth of Hair Follicles - The invention is directed to a chemically defined animal cell culture media, and methods for preparing such a medium, wherein the media are suitable for culturing epidermal cells, preferably human epidermal cells, including cells of the hair follicle. The invention further provides for methods of culturing epidermal cells, hair follicles, and skin explants in the media as well as uses of the cell cultures and explant cultures in screening assays.10-23-2008
20080261258Immune Cell Biosensors and Methods of Using Same - The present invention relates to immunological cells that are useful in detecting changes in physiological states, which provide for methods of diagnosing diseases or monitoring the course of patient therapy. Also provided are arrays of antigen presenting cell-specific markers for detecting changes in physiological states, and methods of detecting such changes.10-23-2008
20080261257Fluorescent Proteins and Related Methods and Compounds - The invention includes fusion polypeptides including a first fluorescent protein, e.g., a FRET donor protein, a second fluorescent protein, e.g., a FRET acceptor protein, and, linked to at least one of the fluorescent (e.g., FRET donor or FRET acceptor) proteins, an Fc-region of an immunoglobulin. The polypeptide can be immobilized with respect to a surface via the Fc-region even in the absence of antibodies to either the FRET donor protein or FRET acceptor protein, and can be used as a calibration standard for fluorescence resonance energy transfer includes a polypeptide.10-23-2008
20090221020Mass Spectrometry Assays for Acetyltransferase/Deacetylase Activity - Provided are methods for determining the activity of proteins that modulate the acetylation state of a protein substrate. The methods may be used for determining both acetyltransferase activity and deacetylase activity. The methods utilize mass spectrometry for determining the acetylation state of a substrate peptide. The methods may also be used to identify compounds that modulate the activity of a protein having acetyltransferase or deacetylase activity.09-03-2009
20090221021DISTINCTION BETWEEN BACTERIAL MENINGITIS AND VIRAL MENINGITIS - The present invention relates to the field of the distinction between bacterial meningitis and viral meningitis. It relates in particular to an in vitro method for detecting the presence of bacterial meningitis, which comprises determining the concentration of procalcitonin present in a test blood sample and of proteins present in a test cerebrospinal fluid sample, and comparing the concentrations thus determined to the concentration of procalcitonin and of proteins present in a reference sample or to a reference value. It also relates to a kit comprising means for detecting procalcitonin and proteins in the cerebrospinal fluid, and to the use thereof for the production of a diagnostic tool for bacterial meningitis.09-03-2009
20090221022Three Dimensional Cell Culture - A method for culturing preadipocytes isolated ex vivo is described, the method including introducing preadipocytes into a three dimensional support matrix, and allowing the cells to differentiate in vitro into adipocytes within the support matrix. The matrix may be a collagen matrix. The method may be used for investigating the development of stem cells, or for investigating the response of adipocytes to stimuli. The method provides a system whereby adipocytes with biological properties resembling those in vivo can be grown in vitro.09-03-2009
20090221024T CELL ASSAYS - The present invention relates to novel T cell assay methods, in particular where T cell responses to test antigens are increased by removal of regulatory T cells. Novel assays where the timing of incubation with antigens or other samples is varied in order to optimize detection of T cell responses are described. The invention has particular application for measurement of human T cell responses to pharmaceuticals, allergens, irritants or other substances.09-03-2009
20090239253METHODS OF USING QUANTITATIVE LIPID METABOLOME DATA - Described herein in various embodiments are methods for using quantitative and/or comparative lipid metabolite data, particularly for identifying and interpreting individual metabolomic profiles as indicative of metabolic status. The provided methods, for instance, allow analysis of the likelihood or progression of weight gain or weight loss, growth or wasting, obesity, diabetes, and aging in an individual based on measurements of the measurement of the quantity of one or more lipid biomarkers, profiles of such markers, or ratios of such markers.09-24-2009
20090239251Method for Detecting Nanoparticles and the Use Thereof - The invention relates to a method for detecting and/or qualifying nanoparticles whose mean size is less than 60 nm, which exhibit a plasmon resonance and are located on the top surface of a flat solid support. A device for carrying out the inventive method and the use thereof are also disclosed.09-24-2009
20090239249NOVEL THERMOSTABLE GLUCONATE DEHYDRATASE AND USE THEREOF - The present invention relates to a novel thermostable gluconate dehydratase from the thermoacidophilic archaeon 09-24-2009
20090253161FLUORESCENT PROCHELATORS FOR CELLULAR IRON DETECTION - Fluorescent probe compound of Formula Ia or Formula Ib:10-08-2009
20090253160NOVEL METHOD OF PREDICTING PIG LITTER SIZE BY EVALUATING SEMEN - Provided is a method of predicting pig litter size by evaluating semen, and more particularly, a method of predicting pig litter size using an in-vitro sperm penetration assay (SPA).10-08-2009
20090263852NONAPOPTOTIC FORMS OF CELL DEATH AND METHODS OF MODULATION - The invention is directed to methods for identifying molecules that modulate paraptosis, a newly identified nonapoptotic form of programmed cell death. The invention also provides a method of inhibiting paraptosis by preventing association of caspase-9 and an endogenous paraptosis-mediating molecule. In one embodiment, the invention provides a method of inhibiting paraptosis by preventing association of caspase-9 and the Insulin-Like Growth Factor I Receptor (IGFIR). The invention further is directed to therapeutic methods for treatment of pathologies associated with aberrant levels of paraptosis such as neural cell death disorders and neoplastic disorders. Also provided by the present invention are methods of identifying endogenous paraptosis-mediating molecules.10-22-2009
20090275069Pancreatic Cancer Genes - The present invention provides the art with the DNA coding sequences of polynucleotides that are up-or-down-regulated in cancer and dysplasia. These polynucleotides and encoded proteins or polypeptides can be used in the diagnosis or identification of cancer and dysplasia. Inhibitors of the up-regulated polynucleotides and proteins can decrease the abnormality of cancer and dysplasia. Enhancing the expression of down-regulated polynucleotides or introducing down-regulated proteins to cells can decrease the growth and/or abnormal characteristics of cancer and dysplasia.11-05-2009
20100015656Arrangement For On-Line Measurements on Cells - The invention relates to an arrangement (a measurement device) for on-line measurements on cells, in particular for measuring soluble analytes and dissolved gases on samples in a sample area.01-21-2010
20100015657METHODS AND DEVICES FOR ASSESSING BIOLOGICAL FLUIDS - The present invention relates to devices, methods, and kits used to determine the source of an aliquot of a biological fluid, including the presence of additives in the biological sample.01-21-2010
20100178662NON-INVASIVE HUMAN-HEALTH-MEASUREMENT SYSTEM AND METHOD - Methods and hand-held devices non-invasively measure a health parameter in an individual or a plurality of individuals. Plural hand-held devices may be provided to plural individuals. Each device may compute a score based on the assayed health parameter in the individual. A server on a computer network may receive the scores and determine a pattern based on the scores. The hand-held devices may be further configured to receive additional data from the individuals regarding activities that may affect the individuals' scores. The server may receive the additional data from the devices and determine additional patterns.07-15-2010
20100178660METHODS AND KITS FOR DETECTING HEMOGLOBIN IN TEST SAMPLES - The present invention relates to methods of detecting hemoglobin in a test sample. These methods can be used to diagnose a subject suffering from a genetic disorder relating to hemoglobin metabolism, to determine the eligibility of a subject to be a blood donor, to determine the age of a stored blood sample or to identify a hemolyzed plasma sample. The present invention also relates to kits for use in the above described methods.07-15-2010
20090275072In Vitro bio-reactor circuit - Embodiments of the invention provide bio-reactor circuits for in vitro research applications. One embodiment provides a bio-reactor circuit comprising at least one bio-reactor and a pump fluidically coupled to the at-least-one bio-reactor. The bio-reactor comprises a housing having inlet and outlet ports and first and second chambers. The chambers are separated by a porous membrane with the first chamber providing a flow path for a fluid. The membrane includes a coating having a cell binding affinity for the attachment and proliferation of cells to cover the surface of the membrane. The second chamber provides a volume for maintaining the viability of cells disposed in the chamber. The cells can be selected to produce a biochemical compound. The membrane is configured to allow for diffusion of the compound from the second chamber into the flow path as well as allow for diffusion of gases, nutrients and other biochemical compounds.11-05-2009
20100151509REAGENT, KIT AND METHOD FOR DIFFERENTATING AND COUNTING LEUKOCYTES - The present disclosure discloses a reagent for differentiating and counting leukocytes which includes: (1) cationic cyanine compounds selected from those having the following general formulae I and II; (2) cationic surfactants selected from those having the following general formulae III, IV and/or V; (3) at least one nonionic surfactant; and (4) optionally, at least one anionic compound selected from those having one or more carboxyl or sulphonyl groups; and optionally includes alcohol compounds. Also disclosed is a kit comprising the reagent for differentiating and counting leukocytes. Further disclosed is a method for differentiating and counting leukocytes using the reagent and kit. Use of the reagent, kit and method disclosed enables the identification of leukocytes in blood samples into five subtypes in a very short time, or at least achieves the differentiation and counting of four leukocyte groupings. Moreover, immature and abnormal cells can be identified.06-17-2010
20110229926STORING METHOD OF MEASURING APPARATUS OF AIRBORNE FLOATING BACTERIA - [Problem] To present a storing method of a measuring apparatus of airborne floating bacteria capable of preventing contamination of the measuring apparatus by airborne floating bacteria and growth of airborne floating bacteria in the measuring apparatus during storage of the measuring apparatus of airborne floating bacteria.09-22-2011
20130137129METHODS FOR NANO-MECHANOPORATION - Methods for creating a transient nanoscale opening in a cell membrane and methods for transporting a desired species through the nanoscale opening are provided. A nano-sized needle-like tip can be used to mechanically slice the cell membrane to create a transient, localized nanoscale slit. The nanoscale slit may be used for transferring exogenous molecules into a living cell05-30-2013
20130137130METHODS AND SYSTEMS FOR CONVERTING PRECURSOR CELLS INTO INTESTINAL TISSUES THROUGH DIRECTED DIFFERENTIATION - The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal al development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.05-30-2013
20130137131System and Method of Producing Volatile Organic Compounds from Fungi - An isolated fungus is described. The isolated fungus produces at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1, 4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone. A method for producing at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1, 4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone is also described. The method includes culturing a fungus on or within a culturing media in a container under conditions sufficient for producing the at least one compound.05-30-2013
20100184120APPARATUS AND METHOD TO CHARACTERIZE BLOOD AND RED BLOOD CELLS VIA ERYTHROCYTE MEMBRANE FRAGILITY QUANTIFICATION - The present disclosure describes an apparatus and associated method for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. This apparatus and the methods of its use are amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions. A vital aspect of this testing system is the accumulation of copious output and other associated data and the mathematical analyses thereof to optimize algorithms by which to characterize each subsequent test output as meaningfully as possible. While the present invention is directed toward applications in blood quality control, the core technology of “quantifying RBC fragility via stress-induced hemolysis and subsequent optical and computational analysis” could have broader application, such as in disease diagnosis.07-22-2010
20120196315METHODS OF DEVELOPING TERPENE SYNTHASE VARIANTS - The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.08-02-2012
20120196314THREE-DIMENSIONAL (3D) HYDRODYNAMIC FOCUSING USING A MICROFLUIDIC DEVICE - A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.08-02-2012
20120196318METHOD AND ASSEMBLY FOR DETERMINING CELL VITALITIES - The method includes binding living cells to magnetic particles, adding them to a sensor array, uniformly distributing over the sensor array, magnetically fixing the magnetic particles having the bound cells over the sensor array, and adding substances to maintain and/or improve the cell vitality to the sensory array, and/or adding substances to worsen the cell vitality to the sensor array. The assembly includes a sensor array composed of sensors, which are designed to be in direct fluidic contact with a fluid, and a device for generating a magnetic field over the sensor array. A layer that comprises magnetic particles and living cells is formed on the sensor array.08-02-2012
20130122534Microfluidic Assay - A process for carrying out hematoxylin and eosin staining in a microfluidic device is described.05-16-2013
20130122535METHODS AND COMPOSITIONS FOR LABELING POLYPEPTIDES - Synthesis of many proteins is tightly controlled at the level of translation and plays an essential role in fundamental processes such as cell growth and proliferation, signaling, differentiation or death. Methods that allow imaging and identification of nascent proteins allow for dissecting regulation of translation, both spatially and temporally, including in whole organisms. Described herein are robust chemical methods for imaging and affinity-purifying nascent polypeptides in cells and in animals, based on puromycin analogs. Puromycin analogs of the present invention form covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized and specifically captured by a bioorthogonal reaction (e.g., [3+2]cycloaddition). The methods of the present invention have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo.05-16-2013
20130122536METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELL INTO INTERMEDIATE MESODERM CELL - The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in a medium containing BMP and Wnt or a functional equivalent of Wnt; to a method for producing a metanephric cell from the intermediate mesoderm cell produced by the first method; to a human pluripotent stem cell having a foreign reporter gene in the chromosome wherein the gene is expressed interlocked with the expression of endogenous OSR1; to a method for screening for an inducer for differentiation into intermediate mesoderm using the human pluripotent stem cell; and to a kit for inducing the differentiation into an intermediate mesoderm cell.05-16-2013
20100261219TEST METHOD OF BIOAVAILABILITY AND BIOEQUIVALENCE FOR XENOBIOTICS USING GENETIC PROFILING - Disclosed is a test method of bioavailability or bioequivalence for xenobiotics, comprising selection of test subjects (human or animal) based on the genetic information for metabolic enzymes or transporters that influence pharmacodynamics or pharmacokinetics for xenobiotics, and testing bioavailability or bioequivalence of the same.10-14-2010
20100261222CELL CULTURE MEASURING SYSTEM AND METHOD FOR COMPARATIVE INVESTIGATIONS ON CELL CULTURES - The invention relates to a cell culture measuring system and to a method for comparative investigations on cell cultures. It is the object of the invention to propose possibilities with which comparative investigations can be carried out on cell cultures under the same conditions on cells which are influenced by a test liquid and on cells which are not influenced by test liquid. In a cell culture measuring system in accordance with the invention, cell cultures are cultivated on a cell culture carrier within a flow passage. An inflow and an outflow for a liquid culture medium and at least two sensors are present. In addition, at least one further inflow for a test liquid is arranged in the flow direction of the culture medium between the inflow for the culture medium and the outflow of the flow passage so that the test liquid flowing through the flow passage by displacement of the culture medium only flows around and/or over some of the cells present on the cell culture carrier. In addition, at least one sensor is arranged in the region of the cells not influenced by test liquid and at least one further sensor is arranged in the region of cells influenced by test liquid.10-14-2010
20100173349Cellular Libraries of Peptide Sequences (CLiPS) and Methods of Using the Same - The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.07-08-2010
20100167331ASSESSING RISK OF CARDIAC INTERVENTION IN PATIENTS SUFFERING FROM STABLE CORONARY HEART DISEASE BASED ON GDF-15 - Described is a method for diagnosing whether a percutaneous cardiac intervention (PCI) in a subject suffering from a stable coronary heart disease was successful, the method comprising determining the amount of GDF-15 in a first sample of the subject which has been obtained after PCI and comparing the determined amount of GDF-15 with a reference amount of GDF-15 which is determined in a second sample of the subject suffering from a stable coronary heart disease obtained prior to PCI, whereby it is diagnosed whether PCI was successful. The invention also relates to the use of means for determining the amount of GDF-15 and, preferably, a natriuretic peptide and/or a cardiac troponin for the preparation of a diagnostic composition for diagnosing whether a PCI in a subject suffering from a stable coronary heart disease was successful.07-01-2010
20100261217Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.10-14-2010
20110177543REGULATION OF BACE DEGRADATION - The invention relates to methods and products for diagnosing, preventing, and treating Alzheimer's disease and abnormal production of amyloid β.07-21-2011
20100184122INDICATOR FOR ASSESSING BODY ODOR, PROCESS FOR PRODUCING THE SAME, BODY ODOR ASSESSMENT METHOD, METHOD OF ASSESSING EFFICACIOUSNESS OF DEODORANT AND KIT FOR CONVENIENTLY ASSESSING BODY ODOR - A method of assessing body odor using as an index an indicator material comprising an alcohol compound having a mercapto group at the 3-position represented by the following formula (2) and/or a substance that is a derivative of an alcohol compound having a mercapto group at the 3-position, wherein an atom(s) or an atom group(s) is introduced to a mercapto group and/or a hydroxyl group of an alcohol compound having a mercapto group at the 3-position represented by the formula (2):07-22-2010
20100261218METHODS FOR PRODUCING SECONDARY METABOLITES - The invention relates to methods for producing secondary metabolites using transformed 10-14-2010
20100261220Biological system and assay for identifying modulators of tubulin ligases - is used as a host cell in a biological assay for identification of modulators of tubulin ligases.10-14-2010
20100261221METHOD TO PREDICT OR DIAGNOSE A GASTROINTESTINAL DISORDER OR DISEASE - The disclosure provides methods and compositions useful for identifying a subject's predisposition to a gastrointestinal disease or disorder.10-14-2010
20090305331Kits and methods for determining colon cleanliness - Method and kits for determining a cleanliness level of a colon of a subject are provided. The methods and kits of the present invention determine a parameter associated with colon cleanliness in a sample obtained from the subject and associate the parameter with the cleanliness level of the colon of the subject.12-10-2009
20100190199GPR30 ESTROGEN RECEPTOR IN BREAST AND OVARIAN CANCERS - A method of prognosis for ovarian or breast cancer patients is carried out by detecting GPR30 in a sample of tissue. An elevation in the level of GPR30 compared to a normal control level indicates presentation of late stage disease and indicates responsiveness of the tumor to hormonal therapy.07-29-2010
20100167332INDUCTION OF DENDRITIC CELL DEVELOPMENT WITH MACROPHAGE-COLONY STIMULATING FACTOR (M-CSF) - A method of inducing dendritic cell (DC) development by administering Macrophage-Colony Stimulating Factor (M-CSF) is provided. M-CSF induces DCs to differentiate into Subtypes, for example plasmacytoid DCs and conventional DCs. Said differentiation is independent of Fms-like-Tyrosine- Kinase 3-Ligand (FL) and/or Granulocyte-Macrophage-Colony Stimulating Factor (GM-CSF). Induction with M-CSF can be achieved in vitro from hematopoietic precursors, such as bone marrow cells, or in vivo. In vitro, M-CSF-derived DCs can be used to produce cytokines and to stimulate other immune response cells. M-CSF can also be used to induce precursor cells removed from an animal to develop into DCs. In addition, these isolated DCs can be exposed to antigens to stimulate a specific immune response when reintroduced into the animal. Treatments for Cancers, such as Acute Myeloid Leukemia, and autoimmune diseases such as Systemic Lupus Erythematosus, are also provided in the invention.07-01-2010
20090269798METHOD AND SYSTEM FOR SELECTING CHEMOTHERAPEUTIC AGENTS - Described herein is a method and corresponding system, including reagents and assays, for determining the in vivo efficacy of drugs in the course of chemotherapy. The method can include identifying a patient having a condition, such as leukemia, that is potentially susceptible to one or more candidate drugs having a particular in vivo biochemical activity, such as the generation of reactive oxygen or nitrogen species. An in vitro biochemical assay is provided that corresponds to the biochemical activity, and a tissue sample is tested to determine the responsiveness of the tissue sample to the biochemical activity, for instance, the ability of the tissue sample to degrade or inhibit the active species (in this case, hydrogen peroxide), and thereby prevent its desired effect against the cancerous cells. A course of chemotherapy can be based, at least in part, on the responsiveness of the tissue sample to the biochemical activity.10-29-2009
20090298116CELL CULTURE APPARATUS HAVING DIFFERENT MICRO-WELL TOPOGRAPHY - A cell culture apparatus includes a substrate having formed therein a micro-well array, the micro-well array comprising a plurality of micro-wells. Each micro-well is defined by a curved surface which is concave.12-03-2009
20110129866NOVEL TISSUE CULTURE PLATFORM FOR SCREENING OF POTENTIAL BONE REMODELING AGENTS - The present disclosure provides methods for identifying candidate compounds having bone anti-resorption activity or bone pro-formation activity. The methods involve the use of ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions in the presence of the candidate compound. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system.06-02-2011
20110129869SELECTIVE ENRICHMENT MEDIUM FOR CARBAPENEM-RESISTANT BACTERIA - The present invention relates to a method for direct detection and differentiation of carbapenem-resistant bacteria in a sample comprising (i) inoculation with said sample of a culture medium comprising at least meropenem and/or ertapenem and at least one chromogenic agent, (ii) incubation of said culture medium under conditions allowing the growth of carbapenem-resistant bacteria, and (iii) detection of colonies formed on said culture medium corresponding to carbapenem-resistant bacteria, as well as a culture medium suitable for use in such a method.06-02-2011
20100184116FLUORESCENT PROTEIN - The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 4607-22-2010
20100227357FUNGAL ISOLATES AND THEIR USE TO CONFER SALINITY AND DROUGHT TOLERANCE IN PLANTS - The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance to inoculated plants, including both monocots and dicots. In particular, 09-09-2010
20110059478Orphan Nuclear Receptor - The present invention relates to a novel human orphan nuclear receptor that binds to a cytochrome P-450 monooxygenase (CYP) promoter and that is activated by compounds that induce CYP gene expression. The invention further relates to nucleic acid sequences encoding such a receptor, to methods of making the receptor and to methods of using the receptor and nucleic acid sequences encoding same. The invention also relates to non-human animals transformed to express the human receptor and to methods of using such animals to screen compounds for drug interactions and toxicities.03-10-2011
20110059477FLUORESCENT DYE-LABELED GLUCOSE BIOPROBE, SYNTHESIS METHOD AND USAGE THEREOF - Disclosed are a novel fluorescent glucose analogue, a method for the synthesis thereof and the use thereof. The novel fluorescent glucose analogue is labeled with fluorescent dye by O-1-glycosylation and via various linkers. The fluorescent glucose analogue can be applied to molecular bioimaging and a method for screening curative or preventive drugs for glucose metabolism-related diseases.03-10-2011
20110059476BIOREACTOR FOR QUANTIFICATION OF HEADSPACE VOC CONTENT FROM CULTURES - A bioreactor for sampling the headspace above cells includes a glass reactor vessel having an interior space and a plurality of valves operatively coupled to the interior space of the glass reactor vessel. The bioreactor includes a sample holder configured to reside in the interior space. The interior space includes a charge of air with a known composition of volatile organic gases and, in some instances, doped with carbon dioxide. The sample holder may hold cells, bacteria, or viruses. The headspace may be sampled after incubation to determine the VOC content or content of specific VOC constituents. Examples include acetaldehyde and hexanaldehyde.03-10-2011
20120196317PORTABLE ENRICHMENT, ALIQUOTING, AND TESTING DEVICE OF MICROORGANISMS AND TOXINS - The present invention relates to devices for conducting microorganism or toxin detection. More particularly, the invention relates to portable, pre-packaged devices that are suitable for culturing microorganisms, aliquoting predetermined volumes of testing samples, and conducting microorganism or toxin detection based on immunological reactions using samples of considerable size collected at remote sites away from testing laboratories.08-02-2012
20120196312CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING THE STEM CELLS - The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.08-02-2012
20100221769ELECTROPORATIVE FLOW CYTOMETRY - Novel devices and methods are provided, which include electroporative flow cytometry, where electroporation is combined with flow cytometry. The devices and methods can be used for the detection a variety of cellular features, including protein translocation, and for monitoring biomechanics at single cell level. Using the novel devices methods it is possible to observe the release of proteins such as intracellular kinases out of the cells during electroporation. Using the novel devices methods it is possible to study cytoskeleton dynamics and deformability at a single cell level, and to correlate these to diseases such as cancers.09-02-2010
20100221768CELL CULTURE DISH - An object of the present invention is to provide a culture dish appropriate for automatically identifying cultured cells. The present invention relates to a culture dish for culturing cells that require separate control, which has a bottom wall and a side wall, wherein cell-holding parts having wells are arranged on the bottom wall, 4 or more wells are adjacent to each other, the wall surface of each well has a concave surface that slopes upward from the lowest position to the outer edge of the well, and the pitch between adjacent wells is 1 mm or less.09-02-2010
20100151508COMPOUNDS - Flavonoid-type compounds of the Formula (II); in which R06-17-2010
20100240087METHOD AND SYSTEM FOR THE AUTOMATIC DETECTION AND DIAGNOSIS OF A CANCER STEM CELL - A method for predetermining whether a cancer has a probability to become metastatic or recurrent, having the steps of: obtaining a sample cell population; assaying the sample cell population; detecting the rate of change of the sample cell population's pH over time; and comparing the pH change versus a predetermined pH rate of change. Also provided is a method of treating a patient having the steps of: isolating a cancer stem cell from a patient; culturing the cancer stem cell to produce a pool of descendant cells; culturing cells from the pool of descendant cells in the presence at least one compound among: anti-cancer drugs, myeloablative, chemotherapeutic, and immunotherapeutic agents, and a combination thereof; assaying over time, during the step of culturing, hydrogen ion concentration in the set of cells; and selecting a candidate therapeutic regimen for the patient based on a result of the assaying step.09-23-2010
20100216179Method for detecting cancer and reagents for use therein - A method for detecting cancer includes providing a biomedical sample, performing a first adsorption step, performing a first desorption step, and performing a first discrimination step. The first adsorbing step includes immersing the biomedical sample into a first detection reagent having a first adsorbent that adsorbs onto the biomedical sample. The first desorption step includes immersing the biomedical sample into a first desorbing agent for a first period of time. The first discrimination step includes measuring an amount residual of first adsorbent adsorbed on the biomedical sample for identifying distribution of cancer cells within the biomedical sample. The present invention could be widely applied for detecting various cancers based on the differential physisorption of adsorbent. The present invention can provide a method for rapidly and non-invasively detecting cancers.08-26-2010
20100216181METHODS AND COMPOSITIONS FOR THE DIFFERENTIATION OF STEM CELLS - The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.08-26-2010
20100255525Fluorescent ozone sensor - A selective, fluorescent “turn-on” probe and method for the detection of ozone in biological and atmospheric samples, wherein the method of detecting ozone in a sample comprises the steps of (1) contacting the sample with a fluorophore capable of undergoing allylic ether or allylic ester cleavage and (2) detecting fluorescence in the sample.10-07-2010
20100255526BIOREACTOR PROBE CONNECTION SYSTEM - A system providing a sterile connection between a sensor probe and a fluid processing apparatus (e.g., a bioreactor) includes a first probe receiving element mountable to the fluid processing apparatus and a second probe receiving element having a gas-permeable contaminant barrier material and coupleable to the first probe receiving element. A sensor probe may be mounted to the second probe receiving element, with the combination being sterilized with a sterilant gas such as steam. Following such sterilization, connection between the first and second probe receiving elements is made through matable sterile couplings, and the probe is insertable through the coupled receiving elements to a position in fluid contact with the interior of the fluid processing apparatus.10-07-2010
20100227356METHODS FOR ASSESSING EFFECTS ON SKELETAL GROWTH - A method for assessing effects on growth of skeletal members comprises preparing a culture comprising a skeletal member and a substance whose effect on growth of the skeletal member is to be determined and capturing images of the skeletal member at selected time intervals without terminating growth. The skeletal member may be fully articulated and the substance whose effect on growth is to be determined may be labeled with a marker and tracked over time. The methods of the invention may be used to assess effects on growth through the positioning of the substance in the skeletal member and for drug discovery.09-09-2010
20100159501METHOD FOR QUANTIFICATION OF CELLULAR SPHINGOLIPIDS - A method is provided for quantifying endogenous sphingolipids in a biological system. The method includes preparing one or more isotope labeled amino acids; introducing the isotope labeled amino acids into a biological system; extracting and separating a sphingolipid-containing fraction from the biological system; and quantifying the amount of endogenous sphingolipids in the biological system. The isotope-labeled amino acid may include a non-essential amino acid, and the method may further include adding an amino acid synthesis inhibitor into the biological system. Systems and kits for quantifying endogenous sphingolipids also are disclosed.06-24-2010
20100240086BIOCHIP ASSEMBLY AND ASSAY METHOD THEREOF - The present invention is directed to a biochip assembly comprising a semi-permeable membrane and an assay method using said biochip assembly for carrying out cell based assays.09-23-2010
20100240088Peptide Markers for Diagnosis of Angiogenesis - The present invention relates to a method for detecting physiological or pathological blood vessel formation, preferably glioma activity, comprising determining the expression level of colligin 2 in blood, cerebrospinal fluid or tissue vasculature. The invention further relates to the use of a method for detecting physiological or pathological blood vessel formation wherein said use is for monitoring a disease process; a healing process; or a response to a disease therapy.09-23-2010
20100240089Lead isotope tracer method to determine bone mineral turnover - A method of determining bone mineral turnover in bone of a subject involves determining isotopic ratio of one lead isotope (preferably 09-23-2010
20120142042Selective Delivery Of Molecules Into Cells Or Marking Of Cells In Diseased Tissue Regions Using Environmentally Sensitive Transmembrane Peptide - A polypeptide with a predominantly hydrophobic sequence long enough to span a membrane lipid bilayer as a transmembrane helix (TM) and comprising one or more dissociable groups inserts across a membrane spontaneously in a pH-dependant fashion placing one terminus inside cell. The polypeptide conjugated with various functional moieties delivers and accumulates them at cell membrane with low extracellular pH. The functional moiety conjugated with polypeptide terminus placed inside cell are translocated through the cell membrane in cytosol. The peptide and its variants or non-peptide analogs can be used to deliver therapeutic, prophylactic, diagnostic, imaging, gene regulation, cell regulation, or immunologic agents to or inside of cells in vitro or in vivo in tissue at low extracellular pH.06-07-2012
20130217059FLUORESCENT DYES - The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.08-22-2013
20100255524METHOD FOR STABILISING A BIOLOGICAL SAMPLE - The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula:10-07-2010
20100255523Device and method for determining the concentration of a substance - Described are methods for determining a concentration of a substance in a compartment comprising exciting an endogenous compound, or a functional part, derivative, analogue or precursor thereof, measuring the lifetime of the luminescence and/or transient absorption exhibited by the compound, functional part, derivative, analogue and/or precursor, and correlating the lifetime with the concentration of the substance.10-07-2010
20090104646Antibodies And Methods For Generating Genetically Altered Antibodies With Enhanced Effector Function - Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production. The invention also provides methods for increasing the effector function of monoclonal antibodies and monoclonal antibodies with increased effector function.04-23-2009
20100143959GRATING BASED SENSOR COMBINING LABEL-FREE BINDING DETECTION AND FLUORESNCE AMPLIFICATION AND READOUT SYSTEM FOR SENSOR - A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. Various applications for the biosensors are described, including cell-based assays for assessing the effect of drug compounds, proteins, peptides and other materials on cell function. A biosensor embodiment optimized for a luminescent response at two different wavelengths is also described. Such luminescent response could be produced by fluorescence (either native or from an attached fluorophore), phosphorescence, chemi-luminescence, or other luminescence technology. Two different luminescence technologies could be combined on the same biosensor chip.06-10-2010
20100099136Microfluidic Assay for Selection and Optimization of Drug Delivery Vehicles to Tumors - An apparatus and method for assaying a tumor drug delivery vehicle comprises a synthetic microvascular network of interconnected flow channels in fluid communication through a porous wall with a tissue space containing animal cells and means for quantifying drug delivery from the microvascular network to the animal cells.04-22-2010
20100136604IN VITRO TUMOR ANGIOGENESIS MODEL - Provided is a method of inducing tubulogenesis in normal endothelial cells comprising co-culturing the normal endothelial cells with tumor cells and forming tubules from the normal endothelial cells.06-03-2010
20100136602SOUR TASTE RECEPTOR COMPOSITIONS AND METHODS - The present invention relates to sour taste receptors and compositions and methods thereof. In particular, the present invention provides assays and methods of screening for ligands specific for sour taste receptors. Additionally, the present invention provides methods for screening for accessory proteins and mutations, polymorphisms and other potential sour taste receptor protein mutations that are associated with disease states, and therapeutic agents, ligands, and modulators of such proteins. The present invention also provides compositions and methods for modulating sour taste receptors in vitro and in vivo.06-03-2010
20090042237Aptamer based point-of-care test for glycated albumin - This invention describes a point-of-care or home use device for measuring the ratio of glycated albumin to total albumin in saliva and other body fluids. Diabetics and prediabetics may have elevated levels of glucose in their blood that can react with plasma albumin to form glycated albumin. The amount of glycated albumin formed is directly correlated with the level of plasma glucose that the albumin has been exposed to over a period of time. Saliva albumin is derived from plasma albumin and therefore contains glycated and non-glycated albumin fractions that can be measured. The ratio of glycated albumin to total albumin in saliva will provide an indication of the average amount of protein glycation that occurred over the preceding 2-3 week period.02-12-2009
20090042235PHOTOREGULATED PEPTIDE, AND METHOD FOR REGULATION OF PEPTIDE-PROTEIN COMPLEX FORMATION USING THE PHOTOREGULATED PEPTIDE - The present invention provides a method of photoregulating the formation of a peptide-protein complex by using a novel peptide in which a structural change for recognizing a protein is photoregulated, in the formation of a complex between a peptide capable of recognizing a protein and a protein of interest. A peptide having an intramolecular cross-linkage via a photocleavable cross-linking group, and method for producing the same. Further, a peptide which has an intramolecular cross-linkage via a photocleavable cross-linking group and forms a cyclic structure together with the cross-linking group, containing a peptide having an epitope corresponding to a functional protein or a ligand at a part which forms the cyclic structure, and method for producing the same. A reaction regulation method comprising the steps of: irradiating a peptide which comprises a peptide having the above epitope, with light to cause cleavage of the photocleavable cross-linking group, whereby dissociating the cyclic structure; and initiating a reaction between the peptide in which the cyclic structure has dissociated and the functional protein or the ligand to form a complex.02-12-2009
20120196313DEVICES AND METHODS FOR ENHANCED CELLULAR SAMPLE TRANSFER - Described are embodiments of a biological specimen collection transfer and container system and method for handling and processing specimens of cellular, and related particulate matter, directly into a vial or other like container. The vial, or container, assembly includes a series of ridges, protrusions, or like engagement members, that extend from the inside of the vial or container. Application of an external force between the collection apparatus and the engagement members contained within the vial, or container, results in an increased amount of cells, cellular structures, related material, and other particulates to transfer from the collection apparatus to the vial, or like container, thereby improving the sample yield and processing effectiveness of the sample in order to increase diagnostic utility.08-02-2012
20090075321FIBRE OPTIC SENSOR - A sensor for measuring the concentration of an assay substance, such as oxygen in tissue. The sensor comprises an optical fibre (03-19-2009
20090075322METHOD OF ANALYZING VARIOUS SURFACE CHEMISTRIES FOR CULTURING A GIVEN CELL LINE - In one embodiment of the invention, there is provided a method of analyzing various surface chemistries for culturing a particular cell line, which includes the steps of: (1) providing a plasma polymerized surface having first and second regions, the first region including a first concentration of carboxylic acid groups in the plasma polymerized surface and the second region including a second concentration of carboxylic acid groups in the plasma polymerized surface, wherein the first and second concentrations are different; (2) culturing cells from the cell line on the plasma polymerized surface in each the region; (3) observing activity of the cultured cells in each the region; and (4) analyzing the activity of the cultured cells in each region.03-19-2009
20120142044METHODS FOR IONOPHORICALLY SCREENING PORE FORMING BACTERIAL PROTEIN TOXINS AND RECEPTORS - One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors for insect toxicity. The method includes: a) forming a membrane comprising a lipid and an insect receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane. Also provided are a method and kit for determining the amount of live pore forming bacterial toxin protein in a sample.06-07-2012
20120034645METHOD AND APPARATUSES FOR CONDUCTING ASSAYS - Disclosed are methods for conducting assays of samples, such as whole blood, that may contain cells or other particulate matter. Also disclosed are systems, devices, equipment, kits and reagents for use in such methods. One advantage of certain disclosed methods and systems is the ability to rapidly measure the concentration of an analyte of interest in blood plasma from a whole blood sample without blood separation and hematocrit correction.02-09-2012
20120034642METHOD FOR OBTAINING A SINGULAR CELL MODEL CAPABLE OF REPRODUCING IN VITRO THE METABOLIC IDIOSYNCRASY OF HUMANS - The method is based on the use of expression vectors coding for the sense and anti-sense mRNA of the Phase I and Phase II drug biotransformation enzymes showing a greatest variability in humans for transforming cells expressing reductase activity. Such vectors can modulate (increase or decrease) the individualized expression of an enzyme without affecting the other enzymes. This singular cell model can reproduce in vitro the metabolic idiosyncrasy of humans. It is applicable in the study of development of new drugs, specifically in the study of metabolism, potential idiosyncratic hepatotoxicity, medicament interactions, etc., of new drugs.02-09-2012
20100099131SOLUTION OF MATRIX - A solution of a matrix contains a liquid mixture consists of acetonitrile and water as main solvent component, wherein a volumetric ratio of acetonitrile and water is within a range of from acetonitrile 6.5: water 3.5 to acetonitrile 8: water 2, and wherein sinapinic acid is contained as the matrix.04-22-2010
20100203575In Vitro Beating Heart Model - The invention relates to a method for preparing a heart organotypic slice culture, comprising; a) providing a slice of heart, b) placing the slice of step a) on the upper surface of a semiporous support which is permeable to a culture medium, c) placing the support of step b) onto a culture medium, the slice being fed through the semi-porous support by capillarity.08-12-2010
20120196316ANALYSIS OF OVA OR EMBRYOS WITH DIGITAL HOLOGRAPHIC IMAGING - Method for analyzing a sample comprising at least one ovum or embryo, the method being based on digital holographic imaging, the method comprising the following steps: creating at least one object beam and at least one reference beam of light, where said at least one object beam and said at least one reference beam are mutually coherent; exposing said sample to said at least one object beam; superimposing said at least one object beam that has passed through said sample with said at least one reference beam and thereby creating an interference pattern; detecting said interference pattern, called hologram; reconstructing phase and/or amplitude information of object wavefront from said interference pattern; and constructing at least one ovum or embryo analysis image and determining at least one ovum or embryo quality showing parameter from said phase and/or amplitude information.08-02-2012
20090275074System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors - The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.11-05-2009
20100221770Methods for Detecting and Analyzing N-Glycolylneuraminic Acid (Neu5Gc) In Biological Materials - The present application is in the field of sialic acid chemistry, metabolism and antigenicity. More particularly, the present invention relates to the detection and analysis of the non-human sialic acid, N-glycolylneuraminic acid (Neu5Gc) in biological materials, such as food and clinical specimens. Such detection and analysis is facilitated by the use of Neu5Gc specific antibodies. The present invention also relates to the detection of antiNeu5Gc antibodies in clinical samples, as well as the production of anti-Neu5Gc specific antibodies.09-02-2010
20110129865METHOD FOR MARKING MATERIALS - The invention relates to a marking system for marking objects wherein said system comprises a microparticle comprising a cross-linked polymer and a marker component wherein the release of said marker component is triggered by contact of the microparticle with an external stimulus and wherein said polymer is a carbohydrate or a protein.06-02-2011
20090286276METHODS FOR PREDICTING A PATIENT'S RESPONSE TO EGFR INHIBITORS - The present invention provides methods for individualizing chemotherapy for cancer treatment, and particularly for evaluating a patient's responsiveness to one or more epidermal growth factor receptor (EGFR) inhibitors prior to treatment with such agents. Particularly, the invention provides an in vitro chemoresponse assay for predicting a patient's response to an EGFR inhibitor, such as an EGFR tyrosine kinase inhibitor or a molecule targeting the extracellular domain of EGFR. The method generally comprises culturing malignant cells from a patient's specimen (e.g., biopsy specimen), contacting the cultured cells with an EGFR inhibitor that is a candidate treatment for the patient, and evaluating the cultured cells for a response to the drug. In certain embodiments, monolayer(s) of malignant cells are cultured from explants prepared by mincing tumor tissue, and the cells of the monolayer are suspended and plated for chemosenstivity testing. The in vitro response to the drug as determined by the method of the invention is correlative with the patient's in vivo response upon receiving the EGFR inhibitor during chemotherapeutic treatment (e.g., in combination with other standardized or individualized chemotherapeutic regimen).11-19-2009
20100267077 METHOD OF MEASURING THE ABILITY OF A SAMPLE TO WITHSTAND REACTIVE OXYGEN SPECIES (ROSS) - A method of measuring the ability of a biological sample to withstand reactive oxygen species (ROSs). The method includes at least the steps of putting the sample for testing and a photosensitive agent in a liquid medium into contact so as to form a mixture for testing, subjecting the mixture for testing to a dose of light irradiation so as to give rise, by photochemical reaction, to the production of reactive oxygen species, then after irradiation, adding a compound that reacts colorimetrically in the presence of reactive oxygen species (ROSs) to form a chromogen or fluorescent substance, and measuring the quantity of chromogen or fluorescent substance that is produced, and also subjecting a reference mixture to the same photochemical, colorimetric, and measurement reactions as the mixture for testing.10-21-2010
20100196949SAMPLE WELL STRIP - A multiple cuvette strip comprises a plurality of wells and a reversible interlocking device. The well strips can be reversibly interlocked to other well strips to form a sample holder system. One embodiment of a well strip comprises a flange and a slot to form a reversible interlocking device.08-05-2010
20100196950AUTOMATED SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS - An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. The method of the present invention comprises feeding seeds individually to a sampling station, removing a sample from the seed in the sampling station; conveying the sample to a compartment in a sample tray, and conveying the seed to a corresponding compartment in a seed tray. The samples can be tested, and the seeds can be sorted according to the results of the testing of their corresponding samples.08-05-2010
20100196946DEVICE AND METHOD FOR CONDITIONING BIOLOGICAL CELLS - A conditioning device (08-05-2010
20100196944METHOD OF BIOASSAYING YOKUKANSAN - To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells.08-05-2010
20100196948METHOD OF SCREENING BAFF SUPPRESSOR OR INHIBITOR - A method of screening a novel BAFF suppressor or inhibitor. More specifically speaking, a method which comprises adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell to thereby induce the production of BAFF by the cell; a method of screening a substance capable of suppressing the expression or activity of BAFF which comprises adding a test substance to a BAFF-production system prepared by adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell and measuring the expression amount and/or the activity of BAFF in the BAFF-production system; and a BAFF production inducer for a BAFF-producing cell which contains a combination of TPA with ionomycin and/or an anti-CD3 antibody.08-05-2010
20110027820ENGINEERING ENZYMES THROUGH GENETIC SELECTION - The embodiments of the present disclosure provide a versatile system, and methods of using, that allow for the selection of variant nuclear receptor ligand binding domains, or for the selection of variant enzymes, or combinations thereof, that may have an enhanced ability to synthesize a nuclear receptor ligand or a precursor thereof. The present disclosure provides yeast cells comprising: a yeast transcription modulating system comprising a nucleic acid expression system encoding a nuclear receptor ligand-binding domain operably linked to a DNA-binding domain, a second nucleic acid expression system encoding an adapter polypeptide comprising a coactivator domain operably linked to a yeast transcriptional activator, a heterologous enzyme system for generating a nuclear receptor ligand, and a selective genetic locus expressed in the presence of the recombinant nuclear receptor polypeptide and a nuclear receptor ligand specifically bound to the recombinant nuclear receptor polypeptide. The present disclosure further methods of use of the modified yeast cell system for identifying variant nuclear receptor ligand binding domains or variant enzymes synthesizing a nuclear receptor ligand.02-03-2011
20110027821METHOD OF BIOASSAYING YOKUKANSAN - The invention intends to find out a bioassay system with an in-vitro test capable of ensuring the higher quality of yokukansan, and provides a bioassay method for yokukansan, comprising adding glutamate in an amount sufficient to induce cell death and yokukansan to a medium for culturing cells, and evaluating pharmacological activity value of yokukansan from viability of the cultured cells in the medium.02-03-2011
20090325213DIAGNOSTIC AND THERAPEUTIC APPLICATION OF CTL AND NK FUNCTIONALLY SELECTED CELLS - The invention proposes a novel therapeutic and diagnostic methodology based on the use of effector cells (for example CTL and NK cells) selected for being able to induce lysis in target cells. In particular, the invention teaches how to select strains of effector cells of the immune system according to their lytic properties. It also teaches how diagnostic procedures for checking the activity of therapeutic vaccines can be improved with respect to what shown. Finally, it shows how cell therapies used at present can be improved by using this approach.12-31-2009
20090275073DIAGNOSIS DEVICE AND DIAGNOSIS METHOD - A diagnosis device includes: a pretreating portion configured to suspend tissue to obtain a suspension; and an analyzing portion configured to analyze the suspension.11-05-2009
20100221765MICROTUBE READER DEVICE FOR THE ANALYSIS OF BLOOD SAMPLES - The invention comprises taking colour pictures of back-lighted analysis microtubes and using the colour information in the images to identify areas of the image which are relevant for interpreting the result of other possible artefacts, as well as detecting abnormal samples and/or reactions characterised by changes in the colour properties of the reaction.09-02-2010
20090239250METHOD FOR THE ANALYSIS OF CELLS - The present invention provides an improved method of analysing and obtaining reliable data about the plasma membrane of single cells, using a microfluidic cell analyser. The microfluidic cell analyser of the invention comprises a single cell trap, a manipulator arranged to manipulate the outer surface of a cell in the trap, a detection zone in communication with the single cell trap and a detector.09-24-2009
20090136989Method of Measuring the Activity of G(alpha)i-or G(alpha)o-Coupled Receptors Using Ca2+ Influx in Cells - A method of measuring the activation or deactivation of G(alpha)i- or G(alpha)o-coupled receptors, and methods of identifying agonists or antagonists of such receptors.05-28-2009
20100221766NUCLEAR TRANSFER PROMOTER FOR Cdc42 PROTEIN AND METHOD OF SCREENING THE SAME - A nuclear transfer promoter for Cdc42 protein comprising an isoprenoid synthesis inhibitor and/or a geranylgeranyl transferase inhibitor such as an HMG-CoA synthase inhibitor, an HMG-CoA reductase inhibitor, an AMPK activator or a farnesyl pyrophosphoric acid synthase preparation; utilization thereof; a method therefor; a blood vessel remedy comprising the nuclear transfer promoter for Cdc42 protein as the active ingredient; and a method of screening a blood vessel remedy which comprises assaying the ability of Cdc42 protein to transfer into nucleus.09-02-2010
20090148885Fibroblast Growth Factor-Like Polypeptides - The present invention provides novel Fibroblast Growth Factor-like (FGF-like) polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing FGF-like polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with FGF-like polypeptides.06-11-2009
20090142791Method and Apparatus for Detecting an Analyte - Embodiments of the present technique facilitate the detection of analytes. Detection is generally achieved by binding agents labeled with non-radiative energy transfer acceptors. The binding agents, when bound to a specific analyte, change conformation such that the distance between the respective non-radiative energy transfer acceptors and a respective quantum dot or quantum well changes. Certain embodiments of the present technique also comprise readout circuitry configured to determine rates of non-radiative energy transfer electrically and not optically.06-04-2009
20090111138Means and methods for the differentiation of cardiac and pulmonary causes of shortness of breath - The present invention relates to a method for differentiating in a subject suffering from chronic shortness of breath (dyspnea) between (i) a pulmonary disease, (ii) a cardiovascular complication, (iii) a cardiovascular complication accompanied by a pulmonary disease and (iv) dyspnea without cardiovascular or pulmonary causes. The method comprises the steps of determining an amount of a pulmonary surfactant protein in a sample of a subject, determining an amount of a natriuretic peptide in a sample of said subject, and differentiating between (i) a pulmonary disease, (ii) a cardiovascular complication, (iii) a cardiovascular complication accompanied by a pulmonary disease and (iv) chronic dyspnea without cardiovascular or pulmonary causes by comparing the amount determined in a) and the amount determined in b) with a reference amount for each. The present invention further provides a device and a kit for carrying out the inventive methods.04-30-2009
20090111139Diagnostic technique for determining oncogenic signature indicative of tumorous growth - A priori knowledge is obtained from known tumor samples, indicative of cellular pathways associated with those known tumor samples. Multiple pathways are found for each tumor. This becomes a priori knowledge. Later unknown tumor samples are then analyzed against the a priori knowledge to find the pathways etc within the unknown tumor samples. Multiple pathways are collected to form an oncogenic signature. The oncogenic signature is used to find a cocktail of multiple treatments that treats each of the multiple pathways.04-30-2009
20080280320Formulation of antigen - The present invention regards methods and formulations for diagnosis, prevention and treatment of disease. More particularly, the present invention teaches methods and formulations for diagnosis, prevention and treatment with antigen in autoimmune disease, allergy, rejection of transplants and cancer. Examples illustrate how the methods of formulations of the invention may be used for diagnosis and amelioration of autoimmune diabetes in which the 65kd isotype of glutamic acid decarboxylase (GAD) is a major antigen.11-13-2008
20080274491Modulation of the Integrin Linked Kinase Signaling Pathway to Promote Cardiac Cell Proliferation and Self-Renewal - Modulation of the integrin-linked kinase (ILK) signaling pathway is used to enhance the remodeling process relevant to a wide range of cardiac diseases. More specifically, a process to instigate beneficial human cardiac hypertrophy or for post myocardial infarction (MI) remodeling comprising illiciting an overexpression of ILK, is described. The ILK signaling pathway is also used as a means for cardiac stem cell proliferation and self-renewal11-06-2008
20090111140SYSTEMS AND METHODS FOR CELL MEASUREMENT UTILIZING ULTRASHORT T2* - The present disclosure is directed to a new technique for MR measurement of ultrashort T04-30-2009
20110117594PROBE CONNECTOR ASSEMBLY AND MEHTOD OF USE - A probe assembly includes a tubular sleeve having a passage extending between a first end and an opposing second end. The tubular sleeve is movable between an extended position wherein the first end and the opposing second end are spaced apart and a collapsed position wherein the first end and the opposing second end are moved closer together. A connector is secured to the second end of the tubular sleeve, the connector having an opening extending therethrough that communicates with the passage of the tubular sleeve, a sealing layer removably covering the opening of the connector. An elongated probe has a first end and an opposing second end, the second end of the probe being positioned within the passage of the tubular sleeve, the second end of the probe being configured to pass through the opening of the connector when the sealing layer is removed therefrom.05-19-2011
20110117593CELL DISPERSION METHOD, CELL DISPERSING AGENT AND CELL MEASUREMENT METHOD - A cell dispersion method, a cell dispersing agent and a cell measurement method, each of which can disperse a cell mass while reducing damage to a cell are provided. Upon dispersing a cell mass, a fluororesin particle is used. A cell mass composed of an aggregated of multiple cells is mixed with a fluororesin particle in a liquid medium, thereby separating the cell mass into individual cells to disperse the cells. In addition, the dispersed cells are measured by flow cytometry, thereby carrying out cell measurement.05-19-2011
20110117592Methods for Natural Product Optimization - Methods and compositions for natural product optimization are provided. In particular, methods and compositions for selecting bacterial strains (e.g., predatory bacteria such as myxobacteria) which produce a desired compound (e.g., antibiotic, antifungal, or anticancer agent) are provided.05-19-2011
20110129863METHODS AND SYSTEMS FOR PROCESSING SAMPLES ON POROUS SUBSTRATES - Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.06-02-2011
20100279333METHODS OF TREATMENT WITH ANTIBODIES TO A CHEMOKINE EXPRESSED IN INFLAMED ADENOID - The present invention provides nucleotide and amino acid sequences that identify and encode a novel expressed chemokine (ADEC) from inflamed adenoid tissue. The present invention also provides for antisense molecules to the nucleotide sequences which encode ADEC, expression vectors for the production of purified ADEC, antibodies capable of binding specifically to ADEC, hybridization probes or oligonucleotides for the detection of ADEC-encoding nucleotide sequences, genetically engineered host cells for the expression of ADEC, diagnostic tests for inflammation or disease based on ADEC-encoding nucleic acid molecules or antibodies capable of binding specifically to ADEC.11-04-2010
20110111445Peptide for Determining Actin Structures in Living Cells - The present invention relates to novel peptides capable of binding to action. The peptides are useful in methods for detecting actin in vitro or in living cells.05-12-2011
20130130300METHOD AND APPARATUS FOR THE NON-INVASIVE MONITORING OF GAS EXCHANGE BY BIOLOGICAL MATERIAL - The invention provides an indirect pressure sensing system for non-invasive measurement of primary pressure in a sealed container, which communicates primary pressure changes from within the container, via a flexible diaphragm, to a secondary chamber wherein there is a defined relationship between the primary and secondary pressures, which enables a pressure sensor in the secondary chamber to generate a signal representing primary pressure in the sealed container, but to remain isolated from the contents of the sealed container. The pressure sensor can provide electrical outputs representing the pressure detected, and the outputs are fed to data processing means capable of producing a measurement of primary pressure. The system can have a liquid culture of cellular material (eg. micro organisms, plant tissue cells, animal cells etc) partially filling the container, whereby the metabolism and/or growth of cellular material causes gas exchanges between liquid and headspace, which can result in primary pressure changes.05-23-2013
20100304423CELL MEASURING VESSEL, EXTRACELLULAR POTENTIAL MEASURING METHOD, AND CHEMICAL TESTING METHOD - The cell observation using a conventional well plate takes much costs. Each well 12-02-2010
20100248292SAMPLE PROCESSING APPARATUS AND SAMPLE PROCESSING METHOD - A sample processing apparatus comprising: an aspiration section for aspirating a sample from a sample container; a sample container take-out/returning section for taking out a sample container containing a sample from a sample rack holding a plurality of sample containers, and for returning the sample container, from which the sample has been aspirated, to the sample rack; a sample processing section for processing the aspirated sample; a transport section for transporting the sample rack to a take-out position for taking out the sample container from the sample rack; and a transport controller for controlling the transport section to transport the sample rack to a processing position for performing a predetermined process on another sample container held by the sample rack when one sample container has been taken out from the sample rack by the sample container take-out/returning section is disclosed. A sample processing method is also disclosed.09-30-2010
20100248289REAGENT PREPARING DEVICE, REAGENT PREPARING METHOD AND SPECIMEN PROCESSING SYSTEM - A reagent preparing device for preparing a reagent to be supplied to a measurement section for measuring a specimen; the reagent preparing device comprising: a reagent preparing section for preparing a reagent including a first liquid and a second liquid, different from the first liquid; a reagent storage container, connected to the measurement section, for storing the reagent prepared by the reagent preparing section; and a controller configured for determining whether or not the reagent stored in the reagent storage container is suppliable to the measurement section based on reagent expiration date information related to an expiration date of the reagent stored in the reagent storage container, is disclosed. A reagent preparing method and a specimen processing system are also disclosed.09-30-2010
20100248290METHODS FOR DETECTING LUNG CANCER AND MONITORING TREATMENT RESPONSE - A method is described for detecting lung cancer comprising detecting an elevated level of a CTAP III-related biomarker in a biological sample from a subject at risk for developing lung cancer. Further, a method is described for predicting risk of developing lung cancer in a subject comprising detecting an elevated level of a CTAP III-related biomarker in a biological sample from a subject. Additionally, a method of monitoring the success of lung cancer treatment with curative intent is described comprising detecting levels of a CTAP III-related biomarker in a biological sample from a subject undergoing treatment for lung cancer for comparison with the a previous level obtained from the subject. Multivariate analysis may be incorporated into these methods, evaluating such clinical, or demographic risk factors as age, sex, smoking history, smoking status, smoking family history, education level, COPD, socio-economic status, body mass index and lung function. Kits for conducting such methods are described.09-30-2010
20100248288DIFFERENTIATION OF CAUSES OF RIGHT HEART FAILURE - The present invention relates to the field of diagnostic means and methods. More specifically, the present invention relates to a method of differentiating between pulmonary embolism and pulmonary hypertension as the cause of right heart failure in a subject comprising determining the amounts of a natriuretic peptide, a cardiac troponin, GDF-15 and endoglin in a sample of a subject suffering from right heart failure and comparing the amounts with reference amounts, whereby it is differentiated between pulmonary embolism and pulmonary hypertension as the cause of the right heart failure. Furthermore, the present invention relates to methods of determining whether a subject suffering from right heart failure is susceptible to a therapy for pulmonary hypertension or pulmonary embolism as well as to a diagnostic device and a diagnostic kit adapted for carrying out the method of the present invention.09-30-2010
20110177546METHOD OF DETECTING BIOACTIVE MOLECULES IN A FLUID SAMPLE - A method of detecting the presence of bioactive molecules in a fluid sample, comprising contacting a solution of a microorganism selected from the group consisting of a monocellular algae and a cyanobacteria with the fluid sample so as to obtain a formulation having a microorganism concentration of 200 000-1×1007-21-2011
20100304428CELL HOLDING METHOD, CELL TESTING METHOD AND CELL HANDLING APPARATUS - It is intended to provide a method for individually arranging and holding, with rapidity and few variations, the number of cells required for bioassay. The present invention provides a cell holding method including: preparing a sheet provided with a plurality of through-holes; and bringing a suspension liquid of cell-supported particles into contact with the sheet, wherein each of the holes has a size that permits only one of the particles to be held therein together with a liquid.12-02-2010
20100304427SUBSTRATES FOR ADHERING, CULTURING AND ASSAYING CELLS - The present invention provides substrates useful for culturing cells under low serum or serum free conditions. The substrates are useful for conducting cell-based assays where there is interference from serum proteins. Methods are also provided for using the substrates of the present invention as well as methods for making the substrates of the present invention.12-02-2010
20100304420BIOREACTOR FOR MESOPHILIC AND/OR THERMOPHILIC FERMENTATION - This invention relates to a bioreactor for producing high rates of hydrogen from plant biomass. It also relates to the rapid screening, selection and isolation of biofilm forming mesophilic and/or thermophilic bacteria or bacteria consortia that generate high levels of hydrogen from plant biomass or from soluble hydrolysates derived from the hydrolysis of cellulosic materials including hemicellulose. The reactor comprises a primary reactor vessel having a bed of hydrogen producing bacteria towards its base located within a secondary reactor vessel which functions as a hydrogen gas collector and as a clarifier and separator. The plant biomass may be one or a mixture of insoluble cellulosic material and a hydrolysate derived from hydrolysis of cellulosic material. In one embodiment the bed of the primary reactor vessel is fluidised by recycling hydrogen gas saturated plant biomass effluent from the secondary reactor vessel to the primary reactor vessel.12-02-2010
20100304419METHOD FOR DETERMINING THE PROGNOSIS OF GASTRIC CANCER - The 14-3-3β protein is used herein as a tumor marker for prognosis in gastric cancer. The method comprises steps of providing a biological sample, qualifying the 14-3-3β protein level in the sample, and comparing the 14-3-3β protein level in the sample with a normal sample. Upon the 14-3-3β protein level in the biological sample is higher than in the normal sample, which represents the patient providing the sample has a poor prognosis. Having the higher sensitivity and specificity, 14-3-3β protein can be used as a tumor marker for prognosis of gastric cancer.12-02-2010
20100304426Analytical Methods for Measuring Synthetic Progesterone - Embodiments relating to methods, processes and systems for measuring progesterone are provided. In particular, methods permit measurement and quantification of synthetic and/or endogenous progesterone from a progesterone-containing blood fluid sample by measuring a progesterone carbon isotope ratio by mass spectrometry and calculating the fraction of synthetic progesterone in the sample from the isotope ratio. Also provided are methods of evaluating bioequivalence of a synthetic progesterone composition using any of the methods provided herein. In an embodiment, methods of precise measurements of plasma levels are described for detection of progesterone analytes such as total progesterone, endogenous animal progesterone, and synthetic progesterone. Correcting for fluctuations in endogenous progesterone levels following application of synthetic progesterone allows a significant reduction in the number of test subjects required to evaluate bioequivalence of a synthetic progesterone composition.12-02-2010
20100304425METHOD FOR THE EX VIVO ANALYSIS OF A CELL AND APPARATUS THEREFORE - The invention provides a method for ex vivo analysis of a cell (12-02-2010
20100304424DIAGNOSTIC AND THERAPEUTIC TOOLS FOR DISEASES ALTERING VASCULAR FUNCTION - The invention relates to diagnostic and therapeutic tools and applications, particularly useful in diseases that affect vascular health and in inflammatory diseases. In particular, said diagnostic and therapeutic tools employ suitable detection or modulation of endothelial glycocalyx.12-02-2010
20100317049Stable Cell Lines and Methods for Evaluating Gastrointestinal Absorption of Chemicals - Nucleic acids and vectors for interfering with the expression of membrane efflux transport proteins in cells that express such proteins are provided. Also provided are cells and cell lines comprising such nucleic acids and vectors. Methods for screening chemicals and biomolecules for gastrointestinal absorption in animals, and kits for practicing such methods are also provided.12-16-2010
20100317046Non-Endogenous, Constitutively Activated Versions of Human G Protein Coupled Receptor: FSHR - The invention disclosed in this patent document relates to transmembrane receptors, particularly to a human G protein-coupled receptor, more particularly to a follicle stimulating hormone receptor (FSHR), and most particularly to mutated (non-endogenous) versions of the human FSHR for evidence of constitutive activity.12-16-2010
20110111449IN VITRO DETERMINATION OF ANALYTE LEVELS WITHIN BODY FLUIDS - A reagentless whole-blood analyte detection system that is capable of being deployed near a patient has a source capable of emitting a beam of radiation that includes a spectral band. The whole-blood system also has a detector in an optical path of the beam. The whole-blood system also has a housing that is configured to house the source and the detector. The whole-blood system also has a sample element that is situated in the optical path of the beam. The sample element has a sample cell and a sample cell wall that does not eliminate transmittance of the beam of radiation in the spectral band.05-12-2011
20100173344METHODS FOR ISOLATING AND USING PITUITARY ADENOMA STEM CELLS AND PITUITARY ADENOMA CELLS - The present invention describes pituitary adenoma stem cells, pituitary carcinoma stem cells, a method of obtaining the stem cells, and a method of using the stem cells. Uses of the pituitary stem cells include but are not limited to producing pituitary hormones and identifying drugs to treat pituitary disease conditions or pituitary-related disease conditions.07-08-2010
20090068701SYSTEMS AND METHODS FOR SCREENING PHARMACEUTICAL CHEMICALS - A method for obtaining a response of a tissue model system to an activator includes contacting a bio-artificial tissue model system with an activator and measuring cellular mechanical response thereto of at least one of contractile force and tissue stiffness. A method for obtaining a response of a tissue model system to an activator includes contacting a bio-artificial tissue model system with an activator and measuring cellular mechanical response thereto of at least one of contractile force and hysteresis.03-12-2009
20090068700Device and methods for monitoring the status of at least one cell - A device and methods for monitoring status of at least one cell, wherein the cell has a membrane forming a substantially enclosed structure and defining an intracellular space therein. In one embodiment of the present invention, the device includes a first substrate having a first surface and an opposite second surface, a second substrate supported by the first substrate, the second substrate having a first surface, an opposite second surface, a body portion between the first surface and the second surface, a first side surface and an opposite second side surface, wherein the body portion defines a first passage between the first side surface and the second side surface and an opening on the first surface of the second substrate and in fluid communication with the first passage, and sidewalls positioned above the first surface of the second substrate. In one operation mode, when a first medium is introduced into the first passage, the intracellular space of the cell is in fluid communication with the first passage with the first medium, a sensor measures the response of the cell to the first medium.03-12-2009
20110117590Heavy Metal Biosensor - Compositions and methods are provided for detection of certain heavy metals using bacterial whole cell biosensors.05-19-2011
20120270260METHODS OF DEVELOPING TERPENE SYNTHASE VARIANTS - The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.10-25-2012
20090035806Method for Identification of Age of an Ancient Ware by Using Microbes - This invention is related to a method for identification of age of the ancient wares, particularly a method for identification of age of the ancient wares by using the microbe. The method of this invention includes the following steps: establish database corresponding to the microbe parameters in wares to be measured; take sample from the wares to be measured; measure the microbe parameters value; compare the obtained parameters value with the standard database to obtain the ware production year. This invention has features of correct and reliable identification of age of the ancient wares, and wide application range.02-05-2009
20090035804CONSTITUTIVELY OPEN hERG (Kv11.1) MUTANT CHANNELS - We disclose a cell having double mutations of the HERG gene that lead to charge reversal amino acid substitutions at residues 466 and 534 of the wild type Kv11.1 channel protein. These double charge reversal mutations result in cells having constitutively open Kv11.1 channels. Such cells could be used in a method of testing development-stage drugs and other compounds for Kv11.1 channel block activity.02-05-2009
20110008819Method of Differentiating Stem Cells - There is provided an improved efficient method for differentiating stem cells into pancreatic endoderm cells and pancreatic hormone expressing and secreting cells which express Pdx-1 and C-peptide. The invention further provides screening methods for detecting factors of interest that will affect the differentiation of the stem cells into pancreatic endoderm cells.01-13-2011
20110008818MICROFLUIDIC DEVICE ADAPTED FOR POST-CENTRIFUGATION USE WITH SELECTIVE SAMPLE EXTRACTION AND METHODS FOR ITS USE - The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.01-13-2011
20110008820METHODS OF DETERMINING DOSE OF IL-31 AGONIST TO TREAT PULMONARY INFLAMMATION - Use of IL-31 agonists, including IL-31, are used to treat agonists are used to treat asthma, airway hyper-responsiveness or allergic rhinitis. The method comprise inhibiting, reducing, limiting or minimizing production of proinflammatory cytokines and include administration of the IL-31 agonist during sensitization or challenge resulting in the asthma, airway hyper-responsiveness or allergic rhinitis state.01-13-2011
20110008816SYSTEM AND METHOD OF VERIFICATION OF A PREPARED SAMPLE FOR A FLOW CYTOMETER - A system and method for a flow cytometer system including a prepared sample fluid with reference beads; an interrogation zone that analyzes the prepared sample fluid; a peristaltic pump system that draws the sample fluid through the interrogation zone; and a processor that monitors a measured volume of sample fluid sampled by the peristaltic pump system and an expected sample volume based on data generated by the analysis of the sample fluid. A system and method is additionally described using an alternative volume sensing fluidic system.01-13-2011
20110008821LABELED MOLECULE FOR PROGNOSING TUMOR GRADE OF HEAD AND NECK CANCER AND METHOD FOR THE SAME - The present invention discloses a labeled molecule for prognosing the tumor grade of head and neck cancer and a method for the same, wherein a 78-kDA glucose regulated protein (GRP78) is adopted as a labeled molecule of head and neck cancer. GRP78 is much more overexpressed in head and neck cancer cells than in non-cancer cells. GRP78 also has relation with tumor size, tumor depth and tumor metastasis. Therefore, the present invention uses GRP78 overexpression as a reference for tumor grading of head and neck cancer.01-13-2011
20110008815MEASURING AMOUNT OF BOUND AND COMBINED NITRIC OXIDE IN BLOOD - Amount of combined nitric oxide or nitric oxide present as iron nitrosyls in a blood sample is determined by directing a low power electromagnetic radiation beam at a blood sample to liberate nitric oxide gas, dissolving the liberated nitric oxide gas and electrochemically detecting amount of dissolved nitric oxide gas.01-13-2011
20110008817MICROFLUIDIC DEVICE HAVING A FLOW CHANNEL WITHIN A GAIN MEDIUM - The present disclosure relates to microfluidic devices incorporating a gain medium, such as a laser gain medium, and methods for their use. Certain embodiments make use of mirrors integrated into the microfluidic device substrate. Other embodiments are also disclosed.01-13-2011
20110244509ApoIII and the Treatment and Diagnosis of Diabetes - The present invention provides methods of identifying candidate compounds for the treatment of type I diabetes comprising contacting pancreatic β cells with an amount of apolipoprotein CIII (“apoCIII”) effective to increase intracellular calcium concentration, in the presence of one or more test compounds, and identifying those test compounds that inhibit an apoCIII-induced increase in intracellular calcium concentration in the pancreatic β cells. The present invention also provides methods for treating patients with type I diabetes comprising administering to the patient an amount effective of an inhibitor of apoCIII to reduce apoCIII-induced increase in intracellular calcium concentration in pancreatic β cells.10-06-2011
20110244508Method for investigating the thrombocyte function of the blood - A method for investigating the thrombocyte function of the blood, and particularly of platelet aggregation, wherein the following steps are carried out: a) cross-flowing an aperture with blood or blood components; b) determining the active radius of the aperture depending on time and c) evaluating the time-dependent modification of the radius as a measure for blood cell and/or thrombocyte function.10-06-2011
20110014643Neuronal Cells Cultured On Microparticles and Methods of Using Same - The present invention provides methods for culturing neuronal cells for transplantation into a subject. The methods include culturing neuronal cells with microparticles to provide a microparticle and neuronal cell culture composition, wherein the microparticles are coated with a compound that provides for attachment of neuronal cells. The present invention also provides methods of screening the cultured neuronal cells as well as kits and systems for use in the same.01-20-2011
20110020858METHOD OF SELECTING AND SEPARATING NORMAL CELLS AND SPECIFIC CELLS BY USING ULTRASONIC WAVES - The present invention relates to a method of selecting and separating normal cells and specific cells by using ultrasonic waves. More particularly, the present invention relates to a method of selecting and separating normal cells and cancer cells, or normal cells and stem cells by using a difference in attenuation coefficients, speed of sound and so on in normal cells and specific cells obtained when ultrasonic waves are radiated. The method of the present invention is capable of selecting and separating normal cells and specific cells (cancer cells or stem cells) in a simple and efficient manner.01-27-2011
20110039292BIOASSAY FOR YOKUKANSAN - Disclosed is a bioassay system in an in-vitro test that enables higher quality guarantee of yokukansan. A bioassay for yokukansan comprises the steps of culturing cells in a culture medium with serum, adding yokukansan to the cells in a serum-free medium, applying ER stress to the cells to induce cell death, and determining a pharmacological activity value of yokukansan from cell viability in the cells.02-17-2011
20110039291Bioremediation of Nanomaterials - The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: 02-17-2011
20100178663Apparatus And Methods For The Detection Of Plasma Metanephrines - Embodiments of the present invention feature the determination of normetanephrine, metanephrine and 3-methoxytyraminein raw human plasma with minimal sample pretreatment using a weak cationic extraction media to provide a selective sample clean-up and HILIC chemistries as indicators of pheochromoacytoma.07-15-2010
20100178666SYSTEMS AND METHODS FOR FOCUSING PARTICLES - Provided is a method of focusing particles. The method includes: providing a suspension of the particles in a suspending medium; and flowing the suspension along a channel, such that the flowing suspension occupies a certain volume that has at least one cross-sectional dimension smaller than 100 μm. The suspending medium has such viscoelastic properties, that flowing the suspension in the channel directs at least some of the particles towards a focus region, enclosed in said certain volume.07-15-2010
20100178664OPTICAL MEASUREMENT METHOD FOR DETERMINING THE PH OF A MEDIUM USING AGELADINE A AS A FLUORESCENT PH INDICATOR - An optical measurement method for determining a pH of a medium includes adding a fluorescent pH indicator to the medium. The pH indicator is based on naturally-obtained or synthesized ageladine A. The pH indicator is irradiated with light of at least one wavelength so as to provide fluorescence excitation of the pH indicator. An emitted fluorescence intensity of the pH indicator is detected as a measure for the pH of the medium.07-15-2010
20100178661AUTOMATIC TISSUE MICROARRAY APPARATUS AND METHOD FOR ARRAYING TISSUE USING THE SAME - Disclosed is an automatic tissue microarray apparatus that includes: a sample module being drivable and providing a space for mounting at least one donor block and at least one recipient block; an extract module being drivable in at least two directions and having at least one punching tip for punching a tissue from the at least one donor block and arraying the punched tissue in the at least one recipient block; and a controller for controlling the driving operations of the sample module and the extract module and, in response to an externally applied command, the punching and arraying operations of the extract module. The present invention securely provides convenience in use with improved work efficiency and contributes to precision of punching and arraying operations.07-15-2010
20110244505Method for In Vitro Blood Testing - Method for determining the presence of a malady in a patient by measuring the degree of reaction of individual types of white blood cells (basophils, eosinophils, neutrophils, monocytes and lymphocytes) in the patient's blood. The degree of reaction of each type of white blood cells is determined by comparing the volumetric size distributions of the types of white blood cells before and after exposure to suspected reactants. Positive results of a change in the volumetric size distribution of the individual types of white blood cells in response to exposure to a specific reactant indicates the presence of a specific malady, such as an allergic reaction to the suspected reactant.10-06-2011
20090142792Biomarkers for the diagnosis of autoimmune disease - Compositions and methods are provided for prognostic classification of autoimmune disease patients into subtypes, which subtypes are informative of the patient's probability of developing clinical symptoms or severe disease. The patterns of circulating blood levels of serum cytokines provides for a signature pattern that can discriminate patients that have a high probability of developing disease from those that have a low probability of developing disease. Assessment of this signature pattern in a patient thus allows improved methods of care and intervention. In one embodiment of the invention, the autoimmune disease is rheumatoid arthritis.06-04-2009
20110111448DEVICE COMPRISING A FIELD OF TIPS USED IN BIOTECHNOLOGY APPLICATIONS - A method for manufacturing a device including a field of micrometric tips, including forming a polycrystalline layer on a support; performing an anisotropic plasma etching of all or part of the polycrystalline layer by using a gas mixture including chlorine and helium, whereby tips are formed at the surface of the polycrystalline layer.05-12-2011
20110111446THIOL DETECTION METHOD - It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1):05-12-2011
20090197295MASKS USEFUL FOR MALDI IMAGING OF TISSUE SECTIONS, PROCESSES OF MANUFACTURE AND USES THEREOF - The present invention relates to masks for use in mass spectrometry, in particular MALDI, tissue section analysis, comprising a plate made of or coated by an opaque material and having a thickness of less than 150 μm, said plate comprising regularly spaced openings, wherein in the plate upper plane, the diameter D of the largest circle comprising only one opening is superior to the diameter d of a mass spectrometer, in particular a MALDI analyzer, laser beam divided by sin Θ, wherein Θ is the mass spectrometer, in particular a MALDI analyzer, laser beam incidence angle with respect to the sample plane. The invention also concerns processes of manufacture of the masks according to the invention, the use thereof for mass spectrometry, in particular MALDI, imaging of tissue sections, and a method for MALDI imaging of a tissue section using said masks.08-06-2009
20110129867Compound Screening Using Chondrocytes - This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing Type II collagen or aggrecan, or other products that are characteristic of the chondrocyte lineage. Chondrocytes and chondrocyte precursor cells obtained according to this disclosure are suitable for use in both research and clinical therapy.06-02-2011
20110129868CELL CAPABLE OF REPLICATING NOVEL HCV REPLICON, CELL CAPABLE OF REPLICATING FULL-LENGTH HCV RNA, AND USE OF THOSE CELLS - According to the present invention, an HCV replicon-replicating cell is produced by a production method including a step of introducing RNA containing an HCV replicon sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. Further, a full-length HCV RNA-replicating cell is produced by a production method including a step of introducing RNA containing a full-length HCV genome sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. The use of these cells enables the construction of an HCV life cycle reproduction system that is derived from a cell line other than the HuH-7 cell line and that has capabilities equivalent to those of an HCV life cycle reproduction system derived from the HuH-7 cell line.06-02-2011
20110117591CHEMICAL PROBE COMPOUNDS THAT BECOME FLUORESCENT UPON REDUCTION, AND METHODS FOR THEIR USE - Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.05-19-2011
20110244507USE OF NEUREGULIN- AS AN INDICATOR AND/OR TARGET - The invention relates, inter alia, to the use of neuregulin-β as a target in a screening method for active compounds, in particular for exerting an influence on changes in calcium concentration which are mediated by glutamate receptors.10-06-2011
20110244506EGFR DEPENDENT MODULATION OF CHEMOKINE EXPRESSION AND INFLUENCE ON THERAPY AND DIAGNOSIS OF TUMORS AND SIDE EFFECTS THEREOF - The invention relates to diagnosis and therapy of tumors utilizing the epidermal growth factor (EGFR) by means of chemical inhibitors or monoclonal antibodies. The invention relates also to skin irritations, preferably skin rash, in conjunction and associated with the treatment of tumor cells that utilize EGF receptor with anti-cancer agents. The invention is also directed to methods of predicting the efficiency of a tumor therapy/tumor response in a patient based on the treatment with EGFR inhibitors, especially anti-EGFR antibodies. The invention further relates to a method of determining the optimum dose of an anti-cancer agent in EGFR related tumor therapy.10-06-2011
20110244504BIOMARKERS OF INFLAMMATION IN BRUCH'S MEMBRANE OF THE HUMAN RETINA - A method of diagnosing a patient with age-related macular degeneration (AMD), by detecting biomarkers in the patient's Bruch's membrane, and diagnosing the patient with AMD. A method of detecting the presence of AMD in a patient, by detecting biomarkers in the patient's Bruch's membrane. A method of detecting inflammation in a patient, by detecting biomarkers in the patient's Bruch's membrane. A method of determining the progression of AMD in a patient. A method of determining efficacy of a treatment for AMD in a patient. A method of determining the presence of AMD in an animal model. A kit for detecting the presence of disease in a patient, including an assay for biomarkers 3-nitrotyrosine and nitro-A2E. An assay of the biomarkers.10-06-2011
20110244503System and Method for Anti-Cancer Drug Candidate Evaluation - The disclosure provides a method of evaluating the ability of an anti-cancer drug candidate to induce apoptosis in a known cancer cell line by placing a single-cell suspension of a known cancer cell line in a well of a plate, adding at least one drug candidate to the well in an amount sufficient to achieve a target drug candidate concentration, measuring the optical density at selected time intervals for a selected duration of time, determining a kinetic units value from the optical density and time measurements, and correlating the kinetic units value with an ability of the anti-cancer drug candidate to induce apoptosis in the cancer cell line if the kinetic units value is positive. A similar method may be used to evaluate the ability of an anti-cancer drug candidate to induce apoptosis in a cancer type.10-06-2011
20090029404MODULATION OF AQUEOUS HUMOR OUTFLOW BY TARGETING VASCULAR-ENDOTHELIAL-CADHERIN IN SCHLEMM'S CANAL CELLS - The present invention provides methods of inhibiting cadherins between Schlemm's canal cells, to a patient suffering from glaucoma as well as screening for substances that inhibit cadherins between the Schlemm's canal cells.01-29-2009
20090246821PEROXIREDOXIN DRUGS FOR TREATMENT OF HIV-1 INFECTION AND METHODS OF USE THEREOF - The invention includes compositions comprising substantially purified peroxiredoxin that are useful in methods for the treatment and prevention of HIV-1 infection. The invention also includes methods for the treatment and prevention of HIV-1 infection comprising contacting a composition of the invention with a human patient. Additionally, the invention includes antibodies and kits useful in the treatment and prevention of HIV-1 infection.10-01-2009
20090311736INTEGRATED APPARATUS AND METHOD TO DETECT INFLAMMATORY STATES PRESENT IN A SAMPLE OF WHOLE BLOOD - Integrated apparatus and method for hematological analyses, wherein the apparatus, comprises, arranged substantially in line and integrated substantially in a single machine, a device (12-17-2009
20090311737METHOD AND DEVICE FOR GENERATING DIFFUSIVE GRADIENTS IN A MICROFLUIDIC CHAMBER - A microfluidic device is described, capable of generating multiple spatial chemical gradients simultaneously inside a microfluidic chamber. The chemical gradients are generated by diffusion, without convection, and can either be maintained constant over long time periods, or modified dynamically. A representative device is described with a circular chamber in which diffusion occurs, with three access ports for the delivery and removal of solutes. A gradient typically forms in minutes, and can be maintained constant indefinitely. Gradients overlapping with different spatial location, and a controlled rotation of a gradient formed by diffusion are demonstrated. The device can also be used to evaluate chemotactic responses of bacteria or other microorganisms in the absence of convective flow.12-17-2009
20100221767DIAGNOSTIC METHOD AND PROGNOSTIC TOOL FOR OSTEOARTHRITIS - The invention relates to a diagnostic method and prognostic tool for osteoarthritis and uses thereof.09-02-2010
20110086379Method of Differentiating Stem Cells - The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture medium having an osmolality less than 340 mOsm/kg. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media for use in inducing the three germ layers.04-14-2011
20100112623Bacteria analyzer, method for analyzing bacteria, and a computer program product - A bacteria analyzer comprising: a sample preparing section for preparing a measurement sample from a specimen; a detector for detecting bacteria contained in the measurement sample prepared by the sample preparing section; and a controller including a memory under control of a processor, the memory storing instructions enabling the processor to carry out operations, comprising: obtaining the distribution state of bacteria detected by the detector; setting a bacteria count region according to the obtained bacteria distribution state; and counting the number of bacteria contained in the set bacteria count region is disclosed. Method for analyzing bacteria, and a computer program product for bacteria analyzer are also disclosed.05-06-2010
20100047849DETERMINATION OF TESTOSTERONE BY MASS SPECTROMETRY - Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d02-25-2010
20100047848Colorimetric determination of somatic cell count in milk - A simple calorimetric in-line quantitative test to measure white blood cell counts in milk samples using a liquid reagent system that simplifies quantitative in-line SCC measurements using a reflectance measuring mode, and a new apparatus, which permits in-line colorimetric analysis.02-25-2010
20110086381METHOD FOR EVALUATION OF FUNCTION OF PHAGOCYTE - A novel convenient method for evaluating the function of a phagocyte is provided. The method assays sCD14-ST, which is a humoral factor specifically produced in phagocytosis by the phagocyte and which is stable enough for use in an assay. Also provided is a method for detecting diseases associated with the phagocytosis by the phagocyte.04-14-2011
20110086375DEVICES AND METHODS FOR PRODUCTION OF CELL AGGREGATES - The present application provides methods and devices for the production and recovery of cell aggregates. In one embodiment, the device is a microwell device with a high density of microwells. The application also provides a device for extracting cell aggregates such as stem cells or embryoid bodies from well plates. Such cell aggregates are used for the differentiation of pluripotent stem cells such as embryonic stem cells, in the fields of developmental biology and regenerative medicine/tissue engineering.04-14-2011
20110086377Bead Manipulations on a Droplet Actuator - A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier. Related methods and kits are also provided.04-14-2011
20100068750METHOD FOR FLUOROMETRICALLY DETERMINING PHOTOSYNTHESIS PARAMETERS OF PHOTOAUTOTROPIC ORGANISMS, DEVICE FOR CARRYING OUT SAID METHOD AND A MEASUREMENT CHAMBER - The invention can be used in biology and for environmental studies using fluorimeters. The inventive method consists in producing exiting light pulses having equal amplitude and modifiable duration, in measuring fluorescent chlorophyll characteristics at a constant background illumination simulating the irradiation intensity of an object during studies in the natural conditions and after the adaption thereof in the dark and in determining the state of a photosynthetic apparatus according to the entirety of fluorescent intensity values. The inventive device comprises the even number of measuring light sources, a current stabiliser of the light sources, the outputs of which are connected to electric inputs of the light sources, the input of which is connected to a control unit and a natural irradiation sensor is connected to the current stabiliser of the light sources through said control unit. A measurement chamber comprises the even number of light sources which are arranged by pairs diametrically oppositely to each other in one plane which is perpendicular to the axis of the body.03-18-2010
20110250630Motif-grafted hybrid polypeptides and uses thereof - Provided herein are hybrid polypeptides that specifically bind to a disease-associated isoform of a polypeptide involved in diseases of protein aggregation. The hybrid polypeptides can be used for diagnosis and treatment of such diseases. In a particular embodiment, a hybrid protein that specifically binds to the infectious form of a prion (PrP10-13-2011
20110244502HORMONE RESPONSIVE TISSUE CULTURE SYSTEM AND USES THEREOF - The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate.10-06-2011
20110091926PERFUSABLE BIOREACTOR FOR THE PRODUCTION OF HUMAN OR ANIMAL TISSUES - A perfusable bioreactor is used for the production of human tissues, animal tissues or tissue equivalents. Their production is based on a structure cultivated in the interior, the interior being enclosed by an envelope and containing at least one inlet and one outlet for a liquid nutrient medium. Accordingly, the bioreactor can be connected to a unit for producing a perfusion pressure of the nutrient medium. This tissue replacement is particularly suitable for use in clinical/therapeutical applications.04-21-2011
20090258383OPTO-FLUIDIC ARCHITECTURE FOR PARTICLE MANIPULATION AND SORTING - This invention provides an apparatus for particle sorting, particle patterning, and methods of using the same. The sorting or patterning is opto-fluidics based, in that particles are applied to individual chambers in the device, detection and/or analysis of the particles is carried out, such that a cell or population whose removal or conveyance is desired is defined, and the cell or population is removed or conveyed via application of an optical force and flow-mediated conveyance or removal of the part.10-15-2009
20090311733Enrichment Unit for Biological Components and an Enrichment Method - A biological component enrichment unit for the isolation, purification and/or determination of a biological component using particles (12-17-2009
20100015658Meter Strip and Method for Lateral Flow Assay Devices - A diagnostic method and associated test kit for detecting an analyte residing in a test sample is provided. The kit includes a housing, and a membrane disposed within the housing having a detection region and a collection region. A blood sample meter is provided having a first end for absorption of a blood sample, a filtering section adjacent to the first end that filters red blood cell components from the blood sample, and a storage section adjacent to the filtering section that receives plasma or serum from the filtering section. An opening in the housing is sized for insertion of the sample meter into the housing such that the storage section of the sample meter is disposed in fluid communication with the collection region of the membrane. The plasma or serum is transferred from the storage section of the sample meter to the collection region of the membrane for subsequent migration to the detection region.01-21-2010
20100055730Methods for the Diagnosis and Treatment of Female Infertility Using Molecular Markers - The present invention features methods of evaluating the fertility of a female subject, or the fertilization competence of an oocyte extracted from an ovarian follicle, using measurements of one or more differentially expressed components of follicular fluid. The methods of the invention include the steps of measuring the level of a component of follicular fluid. Examples of components of follicular fluid useful in the methods of the invention include apolipoprotein IA, apolipoprotein A, apolipoprotein B, apolipoprotein E, prothrombin, CD 133 (prominin), alpha-2 macroglobulin, alpha crystallin B chain, ATP synthase alpha chain, neuropilin, heparin, heparin-like molecules, heparin receptors, bile acids, Aid, CS, Cortisol, Ang1, Ang2, cholesterol and its derivatives, cholesterol receptors, phospholipids, HDL, LDL, VLDL5 chylomicrons, retinoids, carotenoids, retinol-binding proteins, retinoic acid receptors, transthyretins, leptin, fibrin, ADPases, metal ions, and cytokines, e.g., IL-1β, IL-4, IL-5, IL-6, IL-8, IL-IO, IL-12p40, IL-12p70, IL-13, VEGF, VEGF receptors, PlGF, INF-γ, Aid, CS, Ang1, Ang2, TNF-α, C-reactive protein, and angiopoetin, and comparing the measured level to a reference range in order to determine whether the subject is likely to be fertile, or whether the oocyte is likely to be fertilization-competent. The invention also features methods of treatment of an infertile subject, wherein the treatment methods utilize measurements of one or more differentially expressed components of follicular fluid to determine optimal conditions for performing assisted reproductive therapy, e.g., in vitro fertilization.03-04-2010
20090035805C3A Serum-Free Clonal Cell Line and Methods of Use - A serum-free C3A clonal cell line and methods for generating the same are provided. The C3A cell line has a reduced doubling time in serum-free medium compared to a corresponding C3A cell line from which it is derived. Methods using the cells of the serum-free C3A clonal cell line for the production, expression and recovery of harvestable polypeptides, screening compounds for metabolic activity, studying enteric disease and for use in a bio-artificial liver device are also provided.02-05-2009
20100062474METHOD FOR THE PURIFICATION OF AT LEAST ONE TARGET SUBSTANCE THAT IS TO BE IDENTIFIED - Disclosed is a method for purifying at least one target substance that is to be identified and is present or is formed in a cell culture medium when cells are cultivated. In said method, magnetic particles, i.e. beads, to the functionalized surface of which the target substance selectively attaches, are added to the cell culture medium, and the particles to which the target substance is attached are selected out of the cell culture medium by applying a magnetic field. The method is characterized by the following steps: a serum substitute is provided that is obtained from a natural serum and is free or virtually free of low-molecular substances having a maximum mass of 60 kDa, particularly a maximum mass of 10 kDa; the serum substitute is admixed to the cell culture medium which already contains the cells or to which the cells are added; the cells are incubated in the cell culture medium enriched with serum substitute; at least some of the cell culture supernatant formed during the incubation is separated; the cell culture supernatant is filtered by means of an ultrafiltering process so as to obtain a retentate; the beads are supplied in such a way that the functionalized surface of the beads comprises a plurality of dendrimers containing up to 10 branches, i.e. ten generations, the terminal points of the last generation of each dendrimer being modified; the beads and the retentate are admixed to a buffer solution so as to obtain a mixture; the target substances contained in the retentate are incubated and are fixed to the beads; and the magnetic beads are magnetically selected out of the mixture.03-11-2010
20100062475PARTICLE FOR MEDICAL USE, PARTICLE FOR ANLAYSIS AND METHOD OF PRODUCING THE SAME - The object of the present invention is to provide a particle for medical use which has an excellent capability of fixing a biologically active substance and such a chemical/physical stability that the particle is less dissolved or deteriorated in a washing step, and a particle for analysis which has an excellent capability of capturing a biomolecule and such a chemical/physical stability that the particle is less dissolved or deteriorated in a washing step.03-11-2010
20100062478DRUG FOR ANALYSIS OF WATER TRANSPORT FUNCTION OF MEMBRANE PROTEIN IN BIOLOGICAL TISSUE - The present invention is to provide a drug for analysis of a water transport function of a membrane protein in a biological tissue, wherein the abundance of one or both of 03-11-2010
20100062476ASSESSING MAMMALS FOR VASCULAR DISEASES AND VALVULAR DISEASES - This document provides methods and materials related to assessing mammals for vascular disease. This document also provides methods and materials related to assessing mammals for valvular disease. For example, methods and materials for assessing mammals for vascular disease using elevated levels of endothelial progenitor cells expressing a bone-related polypeptide (e.g., an osteocalcin polypeptide) as markers are provided.03-11-2010
20100068751Method and System for Artifact Reduction - A method is presented for obtaining characteristics of a target physical entity by providing an excitation signal to the target physical entity and simultaneously measuring the response of the target physical entity. Analog signal processing is performed on the measured response to eliminate artifacts arising from a signal path outside the target physical entity and determining the characteristics from the signal processed measured response. The excitation signal and the analog signal processing are selected such that after analog signal processing of the measured signal, the analog measured signal contains artifacts which are localized in time.03-18-2010
20100047840Polypeptide markers for the diagnosis of bladder cancer - A method for the diagnosis of bladder cancer (BC) and/or for determining a tumor stage of bladder cancer, comprising the step of determining the presence or absence or amplitude of at least six polypeptide markers in a sample, wherein said polypeptide markers are selected from markers 1 to 836, which are characterized by the values for the molecular masses and migration times (CE time).02-25-2010
20110250632METHODS FOR CONDUCTING CELLULAR ASSAYS - The present invention relates to cryopreserved cell cultures, methods for cryopreserving cells and methods for conducting cellular assays on such cells. A cryopreserved cell culture of the invention comprises a container having at least a surface which is coated with poly-lysine and frozen cells supported on the surface.10-13-2011
20110250629ALCOHOL PRODUCTION PROCESS - The invention relates to the microbial fermentation of gaseous substrates, particularly to methods of mitigating and/or reducing alcohol toxicity effects on a microbial culture at elevated alcohol concentrations during fermentation. The invention relates particularly to microbial fermentation of substrates comprising CO and the effects of alcohol toxicity are reduced or mitigated by maintaining the temperature below the optimum operating temperature by cooling the fermentation broth.10-13-2011
20110177542METHOD FOR ENHANCING CELLOBIOSE UTILIZATION - The present invention relates to methods for improving a host cell's ability to utilize the disaccharide cellobiose. In some embodiments, a transformed cell expresses intracellular β-glucosidase. In other embodiments, a transformed host cell is able to grow on media wherein cellobiose is the sole carbon source. In other embodiments, selection methods are provided which improve a host cell's ability to grow on cellobiose-containing media.07-21-2011
20100068748Fluorescent Ion Indicators for Cadmium and Lanthanide Ion Detection - The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety has the following formula03-18-2010
20090215109APPARATUS AND METHOD FOR MEASURING LUMINESCENCE AND FLUORESCENCE OF TRANSFECTED CELLS OR ORGAN PARTS - An apparatus for measuring photon emissions from marked cells. The apparatus has the following components: a rotatable, at least partly transparent growth chamber/bioreactor (08-27-2009
20090215108Methods of analyzing a biological sample - Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.08-27-2009
20090215107Activator for Transient Receptor Potential Vanilloid 2 Comprising Probenecids - The present invention relates to a method for activation of TRPV2 (transient receptor potential vanilloid 2) using probenecid, more precisely a method for selecting a candidate for TRPV2 blocker using probenecid. Probenecid of the present invention works on TRPV2 specifically so that it facilitates the isolation of sensory neurons expressing TRPV2. Therefore, probenecid of the invention can be effectively used for the studies on TRPV2 mechanisms and the development of a TRPV2 based anodyne.08-27-2009
20090215106METHOD FOR DETECTING INTRACELLULAR INTERACTION BETWEEN BIOMOLECULES - The present invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules, on living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair.08-27-2009
20090215105Tetraazaporphyrin-Based Compounds and Their Uses - Asymmetrically substituted metal-phthalocyanine compounds are disclosed. These compounds and other phthalo-cyanine-derivatives are used in bioimaging, bioanalysis, FRET and quenching techniques, photodynamic therapy, DNA analysis for cells, proteins, tissues and other biological entities, and other applications. Near-infrared fluorescence minimizes matrix effects typically seen in other methods of analyzing biochemical entities in cells, proteins, tissues and other biological entities.08-27-2009
20110097759Gene Expression Markers for Colorectal Cancer Prognosis - A method of predicting clinical outcome in a subject diagnosed with colorectal cancer comprising determining evidence of the expression of one or more predictive RNA transcripts or their expression products in a biological sample of cancer cells obtained from the subject.04-28-2011
20110104739BIOMARKERS FOR AMYOTROPHIC LATERAL SCLEROSIS - The invention provides a method for diagnosing amyotrophic lateral sclerosis (ALS) in a subject, a method for assessing the effectiveness of a drug in treating ALS, and a method for determining the site of onset of ALS in a subject. Each method comprises (a) obtaining a sample from the subject, (b) analyzing the proteins in the sample by mass spectroscopy, and (c) determining a mass spectral profile for the sample. In some embodiments, the method comprises comparing the mass spectral profile of the sample to the mass spectral profile of a positive or a negative standard.05-05-2011
20110250628Hcv replicons containing ns5b from genotype 2b - The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.10-13-2011
20110250631PROTEOGLYCAN DEGRADING MUTANTS FOR TREATMENT OF CNS - The present disclosure relates to the preparation and deletion mutants of chondroitinase proteins and their use in methods for promoting the diffusion of therapeutic composition into tissues and their use for neurological functional recovery after central nervous system (“CNS”) injury or disease.10-13-2011
20110086382ORGAN-ON-A-CHIP-DEVICE - A self-contained organ-on-a-chip device includes at least one medium feed reservoir and at least one organ growth section including at least one organ cavity. The at least one medium feed reservoir is configured to connect to the at least one organ growth section by a microfluidic feed channel and the at least one organ cavity.04-14-2011
20110086378Cartridge For a Biological Sample - An assay device comprising a compartment adapted to receive a sealed removable cartridge, wherein said cartridge is adapted to contain a biologic sample and internally comprises two or more assay locations, wherein said cartridge is adapted to facilitate two or more assays of said biologic sample; and an actuator adapted to interface with said cartridge to transport said biologic sample towards at least one of said assay locations.04-14-2011
20110076710METHOD FOR CHARACTERISING A BIOLOGICALLY ACTIVE BIOCHEMICAL ELEMENT BY ANALASING LOW FREQUENCY ELECTROMAGNETIC SIGNALS - The invention relates to a method for characterising a biologically active biochemical element by analysing low-frequency electromagnetic signals transmitted by a solution prepared from an analysable material sample characterised in that it comprises a pre-filtering stage.03-31-2011
20110081674CONTINUOUS-FLOW DEFORMABILITY-BASED CELL SEPARATION - This invention provides methods utilizing a microfluidic device that can quickly and accurately discern differences in deformability between individual cells and sets of cells and continuously fractionate populations of cells based on their deformability. This information may be important in disease diagnosis and treatment efficacy monitoring. For example such a device may be able to determine the stage of malarial infection by using red blood cell deformability. Additionally, methods of the invention may be used as a tool to screen drugs that can make cells more flexible in diseases such as sickle cell anemia that causes sickle cell crises. The relatively low manufacturing and operation costs of methods of the invention enable this device to be used in resource-limited settings to diagnose and monitor disease.04-07-2011
20110081675Metabolic Biomarkers Of Drug-Induced Cardiotoxicity - The invention provides methods and biomarkers for assessing cardiac metabolic response to pharmaceuticals, environmental agents, chemical compounds and biologic therapies. The invention provides methods for identifying cellular metabolites secreted by primary cardiomyocytes, cardiomyocyte precursor cells, clonal cardiomyocytes derived from adult human heart, immortalized cardiomyocytes, human embryonic stem cell (hESC)-derived cardiomyocytes, human induced pluripotent stem cell (iPS)-derived cardiomyocytes, or any cell displaying cardiomyocyte-specific markers in response to exposure to pharmaceuticals, environmental agents, chemical compounds and biologic therapies that are cardiotoxic. Cardiomyocyte-secreted cellular metabolites provide metabolic signatures of cardiotoxicity, and can be used to screen pharmaceutical agents, lead and candidate drug compounds, biologics, and other therapeutics for cardiotoxic effects.04-07-2011
20110070602Methods and Apparatuses for Measuring Biological Processes Using Mid-Infrared Spectroscopy - This invention relates to methods and apparatuses for measuring biological processes using mid-infrared spectroscopy. The method includes the step of directing a mid-infrared signal into a sample of a biological process during a biologically active phase, and the step of detecting a sample spectrum from the mid-infrared signal to form a sample spectrum. The method includes the step of generating a reference spectrum through a reference media, and the step of combining the sample spectrum and the reference spectrum to form an adjusted sample spectrum.03-24-2011
20110070604ANALYSIS OF EX VIVO CELLS FOR DISEASE STATE DETECTION AND THERAPEUTIC AGENT SELECTION AND MONITORING - Described herein is the analysis of nanomechanical characteristics of cells. In particular, changes in certain local nanomechanical characteristics of ex vivo human cells can correlate with presence of a human disease, such as cancer, as well as a particular stage of progression of the disease. Also, for human patients that are administered with a therapeutic agent, changes in local nanomechanical characteristics of ex vivo cells collected from the patients can correlate with effectiveness of the therapeutic agent in terms of impeding or reversing progression of the disease. By exploiting this correlation, systems and related methods can be advantageously implemented for disease state detection and therapeutic agent selection and monitoring.03-24-2011
20110070603DISC FOR TESTING BLOOD, BLOOD TESTER HAVING THE SAME, AND CONTROL METHOD THEREOF - Disclosed are a blood tester for measurement of temperature of a sample placed in a blood-testing disc using a thermochromic pigment, and a control method thereof. The disclosed blood-testing disc, the blood tester having the blood-testing disc loaded therein, and the control method of the same, are used to measure a light absorbance of the thermochromic pigment in order to determine a temperature of a sample when a biological material such as blood, blood serum, blood plasma, or sputum, is subjected to analysis, so that the test may be executed at a constant temperature. Accordingly, more reproducible and accurate results are obtainable, and temperature measurement of a sample may be simply and effectively attained by an optical analyzer built in the blood tester, without an alternative instrument or sensor for measurement thereof.03-24-2011
20100129849CELL OBSERVATION APPARATUS, CELL OBSERVATION METHOD, AND PROGRAM - The present invention relates to a cell observation apparatus that facilitates the setting of schedules. An observation schedule that has already been registered is presented to a user by a time schedule display unit of a schedule setting screen, and a new observation schedule is acquired in response to an input of set values of photographic conditions by the user. Then determination is made whether the photographing time of this observation schedule and the photographing time of the already registered observation schedule overlap, and when the photographing times are determined to overlap, the photographic conditions including the photographing time of one or both of the observation schedules are changed and the observation schedule is registered. The invention can be applied, for example, to incubators for culturing cells.05-27-2010
20100129847METHOD FOR THE DETECTION OF AMYLOID-B OLIGOMERS IN BODY FLUIDS - The present invention relates to a method for the detection of marker of the Alzheimer's disease, namely the amyloid-β oligomers in human CSF, using a combination of steps including demasking the epitopes responsible for antibody binding on the Aβ peptide oligomers as well as detecting fluorescently marked antibodies binding to said epitopes, preferably by using the FRET technology.05-27-2010
20100129848Rapid Mixing Device for Subsecond Analysis of Cell Surface Kinetics in Flow Cytometry - The present invention provides improved flow cytometry tubes containing ports to allow rapid addition and mixing of reagents with sample during flow cytometry. The present invention further provides rapid mixing cytometry devices and method for their use in rapid reagent mixing during flow cytometry.05-27-2010
20110076711PHOTON REDUCING AGENTS FOR FLUORESCENCE ASSAYS - The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.03-31-2011
20110059480USE OF AN IN VITRO HEMODYNAMIC ENDOTHELIAL/SMOOTH MUSCLE CELL CO-CULTURE MODEL TO IDENTIFY NEW THERAPEUTIC TARGETS FOR VASCULAR DISEASE - Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.03-10-2011
20110151497METABOLOMIC PROFILING OF PROSTATE CANCER - The present invention relates to cancer markers. In particular, the present invention provides metabolites and panels of metabolites that are differentially present in cancer (e.g., prostate or breast cancer).06-23-2011
20110151499APPARATUS AND METHODS FOR CONDUCTING ASSAYS AND HIGH THROUGHPUT SCREENING - The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.06-23-2011
20120149054MEASUREMENT METHOD FOR VIABLE CELL COUNT, AND CULTURE MEDIUM - There are provided a method of identifying the 06-14-2012
20120149049SYSTEM AND METHOD FOR PRESENTING AN AUTOMATED ASSESSMENT OF A SAMPLE'S RESPONSE TO EXTERNAL INFLUENCES - A biological sample is evaluated by an automated apparatus that operates to identify one or more areas of interest within the sample. Each area of interest has a classifiable appearance brought about from the sample's interaction with an external influence under investigation. The apparatus classifies each area of interest by automatically resolving and identifying either the existence of an abnormal feature in that area of interest or a lack of any abnormality in that area of interest. An abnormal feature qualified as differentiable from a control reference unaffected by the external influence by at least one of: shape; area; size; and proximity to other resolved features within the area of interest. The apparatus counts incidences of abnormal features within each area of interest and automatically presents an assessment of the effect of the external influence on the sample based at least in part on the count of abnormal features.06-14-2012
20120149053CARRIER PEPTIDE FRAGMENT AND USE THEREOF - A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including any amino acid sequence selected from SEQ ID Nos. 1, 2, 3, 4, 5, and 6, or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence of the selected sequence identification number, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.06-14-2012
20120149052METHOD FOR OPTICAL MEASURING VARIATIONS OF CELL MEMBRANE CONDUCTANCE - The instant invention refers to an optical method to extrapolate cell membrane conductance by indirect measurement of changes in transmembrane voltage, upon exposure of a cell sample to electric current pulses. The method is advantageously used for evaluating the activity of molecules able to alter, directly or indirectly, membrane permeability. A specific field of application is the screening of candidate compounds putatively acting on ion channel activity. In particular, it is open to the study of all ion channels with no limitations on the mechanisms of activation or to the ion species involved. The method is also advantageously used for evaluating a cell status, namely a differentiative or a pathologic status.06-14-2012
20120149051DEVICES FOR THE PRODUCTION OF CELL CLUSTERS OF DEFINED CELL NUMBERS AND CLUSTER SIZES - Devices for the in vitro aggregation of cells. The devices are characterized by containing special ground cavities allowing cluster formation to take place when a cell suspension is seeded onto the device. Further, the present invention relates to a method for aggregating cells and the use of the devices of the present invention for the aggregation of cells.06-14-2012
20120149050AUTOMATED SYSTEMS AND METHODS FOR PREPARING BIOLOGICAL SPECIMENS FOR EXAMINATION - The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens.06-14-2012
20120202237PARTICLE SORTING APPARATUS AND METHOD - A particle analyzing and/or sorting apparatus and the associated methods. One aspect of the described embodiments relates to an analyzer, or a sorter, having acquisition and sort electronics in the form of a field programmable gate array for processing detected signals. Another aspect relates to a droplet based approach of analyzing and sorting particles and may further include a dynamic element, such a dynamic drop delay. In still another broad aspect, an apparatus and method for dynamically varying other sorting parameters.08-09-2012
20100311103SOLID SUPPORT COATED WITH AT LEAST ONE METAL FILM AND WITH AT LEAST ONE TRANSPARENT CONDUCTIVE OXIDE LAYER FOR DETECTION BY SPR AND/OR BY AN ELECTROCHEMICAL METHOD - One subject of the present invention is a transparent solid support coated with at least one layer of metal and with at least one layer of transparent conductive oxide (TCO), especially tin-doped indium oxide (ITO) in order to form a solid support that can be used at the same time or independently for detection by SPR and by an electrochemical method. The invention comprises a process for producing such supports, especially by cathode sputtering using a device comprising a radiofrequency (RF) generator, this device also being included in the invention. Another subject of the invention is a kit and a method for detection or identification of an organic or mineral compound by surface plasmon resonance (SPR) and/or electrochemical plasmon resonance comprising or using such supports.12-09-2010
20110033886GDF-15 AS BIOMARKER IN TYPE 1 DIABETES - The present invention relates to a method of predicting if a diabetes type 1 patient will suffer from one or more complications selected from cardiovascular complications, terminal renal failure, and death, the method including (a) determining the amount of GDF-15 in a sample of a diabetes type 1 patient; and (b) comparing the amount of GDF-15 determined in step (a) to a reference amount and establishing a prediction. Also encompassed by the present invention are devices and kits for carrying out the aforementioned methods.02-10-2011
20110033884PD-1, A Receptor for B7-4, and Uses Therefor - Disclosed is a method for modulating an immune response by modulating signaling via PD-1. The immune response may be downregulated by increasing signaling via PD-1, or may be upregulated by decreasing signaling via PD-1. Agents which are useful for stimulating signaling via PD-1 to downregulate an immune response include an activating antibody that recognizes PD-1, a form of B7-4 that binds to an inhibitory receptor, and a small molecule that binds to PD-1. Agents which are useful for inhibiting signaling via PD-1 to upregulate an immune response include a blocking antibody that recognizes PD-1, a non-activating form of B7-4, an antibody that recognizes B7-4, and a soluble form of PD-1. Also disclosed is a method for modulating the interaction of B7-4 with an inhibitory receptor on an immune cell. The method comprises contacting an antigen presenting cell which expresses B7-4 with an agent such as a form of B7-4, a form of PD-1, or an agent that modulates the interaction of B7-4 and PD-1. Also disclosed is a method for inhibiting activation of an immune cell via a non-apoptotic mechanism by increasing the activity or expression of PD-1 in the immune cell. The disclosed methods for modulating an immune response are useful, for instance as therapeutic treatment of a subject with conditions that would benefit from immune response modulation. For instance, a condition such as a tumor, a neurological disease, or an immunosuppressive disease, would be treated by upregulating an immune response, and conditions such as a transplant, an allergy, or an autoimmune disorder would benefit from downregulation of an immune response. Assays for identifying compounds which modulate the activity of, or signaling via, B7-4, or PD-1, especially which modulate the binding of B7-4 or PD-1 to a target molecule are also disclosed. Further disclosed are a vaccine and also other compositions which contain agents that modulate signaling via PD-1.02-10-2011
20120034640Isotonic Buffered Composition and Method that Enables Counting of Cells - The present invention discloses multi-purpose metering fluid/rinse reagents for use in automated cellular analyzers that use liquid volumetric metering. The compositions contain a chelating agent, an ionizing salt, optionally a stabilizing ion, a buffer, a non-hemolytic surfactant, and optionally an antimicrobial agent. Advantageously, the compositions produce less than one part-per-million of formaldehyde over the course of one year. Methods for using the compositions are also described.02-09-2012
20100311105METHODS OF DELIVERY OF EXOGENOUS PROTEINS TO THE CYTOSOL AND USES THEREOF - The present invention is directed to a method for delivering exogenous proteins to the cytosol, by binding a target antigen (such as a protein) to a transport factor that contains a fragment of a bipartite protein exotoxin, but not the corresponding protective antigen. Preferably, the target antigen is fused to the transport factor. Preferred transport factors include the protective antigen binding domain of lethal factor (LFn) from 12-09-2010
20100311099USE OF HE4 FOR ASSESSMENT OF BREAST CANCERS - The disclosure relates to use of the HE4 marker to assess breast cancer in a patient. The disclosure also relates to using HE4 and other tumors markers for diagnosis, grading and staging of breast cancers. The disclosure also relates to determining prognosis and treatment effectiveness of a patient who has been diagnosed with breast cancer.12-09-2010
20100311104NOVEL BACTERIA AND METHODS OF USE THEREOF - A novel class of bacteria is described which has improved efficiency in the production of thanol by anaerobic fermentation of substrates containing carbon monoxide.12-09-2010
20100304422In-Vitro Model of the Transformation of Metaplasia to Neoplasia - This invention relates to a novel in-vitro model of transformation of a benign Barrett's cell line to a neoplastic cell line following repeated exposure to acid and bile over the course of about 65 wks, and to the process of using the model to study the transformation.12-02-2010
20100297684ACTIVATIBLE DYES - Novel, activatable dyes, such as photoactivatable dyes, e.g., oxazine dyes, are described. Some of the dyes are targeting dyes that can, e.g., target biomolecules, such as polypeptides, proteins, or nucleic acids. Upon activation, such as by irradiation, the novel dyes rapidly turn on their fluorescence and emit light, such as near-IR light with spatial and temporal precision.11-25-2010
20100297688METHOD FOR DIAGNOSING OR PREDICTING SHORT STATURE IN HUMANS - An improved assay for measuring the release of endogenous growth hormone (GH) is described. The method focuses on determining the presence of an immediate release pool (IRP) of GH, and the extent to which it is rapidly discharged into the circulation of humans, including children. A larger and less labile pool responds continuously to long term stimulation. The method considers that determining the ten minute AUC measurement, i.e., ten minutes after induction of GH release by administering growth hormone releasing hormone (GHRH) will reveal an immediate release pool of GH. As further disclosed herein, the IRP of GH has a higher correlation with peak GH release than the conventional 120 minute time point. Further the AUC11-25-2010
20100297686DEVICES FOR INTRACELLULAR SURFACE-ENHANCED RAMAN SPECTROSCOPY - Provided are surface-enhanced Raman spectroscopy (SERS) devices suitable for intra-subject (e.g., intracellular) observation, which devices may be of nanoscale size. Also provided are related SERS analysis methods.11-25-2010
20110256571PROTEINS - An amino acid sequence is described. The amino acid may comprise a signal sequence that is SEQ ID NO. 1a (or a variant or homologue or derivative or fragment thereof) that is expressed as a fusion protein that comprises a protein of interest. The signal sequence directs secretion of the protein of interest. The protein of interest may be a heterologous protein. Secretion of the fusion protein aids purification.10-20-2011
20100255527IDENTIFICATION OF PATHOGENS IN BODY FLUIDS - Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.10-07-2010
20110159531METHOD AND APPARATUS FOR MEASURING AMOUNT OF SUBSTANCE - Disclosed herein are a method and apparatus for measuring an enzyme amount. The measurement of the enzyme amount is carried out by measuring a transmittance of a reaction mixture containing the enzyme and its substrate over the reaction time period and providing an optical characteristic curve, dividing the optical characteristic curve by a uniform distance in a reaction time axis direction to set a plurality of sections, and selecting one section satisfying preset linear conditions and having a maximum gradient absolute value from the plurality of sections and calculating an enzyme amount from the gradient of the optical characteristic curve in the selected section.06-30-2011
20110212485SYSTEM, METHOD AND DEVICE FOR TISSUE-BASED DIAGNOSIS - The current invention provides devices, methods and systems involving application of energy and/or a liquefaction promoting medium to a tissue of interest to generate a liquefied sample comprising tissue constituents so as to provide for rapid tissue sampling, tissue decontamination as well as qualitative and/or quantitative detection of analytes that may be part of tissue constituents (e.g., several types of biomolecules, drugs, and microbes). In addition, the current invention provides specific compositions of the said liquefaction promoting medium so as to facilitate liquefaction, preserve liquefied tissue constituents, and enable delivery of molecules into tissues. Determination of tissue composition in the liquefied tissue sample can be used in a variety of applications, including diagnosis or prognosis of local as well as systemic diseases, evaluating bioavailability of therapeutics in different tissues following drug administration, forensic detection of drugs-of-abuse, evaluating changes in the tissue microenvironment following exposure to a harmful agent, and various other applications. The methods, devices and systems are used to deliver one or more drugs through or into the site of the tissue to be liquefied.09-01-2011
20110212480USE OF PRIMARY HUMAN CARDIOMYOCYTES - The present invention provides for the use of primary human cardiomyocytes for the testing of agents that have cardiotoxic effects and other modulatory effects on the heart.09-01-2011
20100086962HEMATOLOGY CONTROLS AND METHODS - An integrated hematology control and methods for making the same, including a first cellular component derived from a plurality of processed animal red blood cells other than human blood cells and a second cellular component derived from a plurality of processed animal red blood cells other than human blood cells and a plurality of human blood cells, wherein the control simulates erythroblasts and white blood cells of a human blood sample on an automated blood analyzer.04-08-2010
20100003712BIOMARKER FOR ASSESSING RESPONSE TO CHYMASE TREATMENT - A biomarker that correlates to treatment with drugs that inhibit chymase is disclosed. This biomarker has been shown to have utility in assessing response to chymase inhibitor compounds. The immunoreactivity or levels of the biomarker is increased upon treatment with chymase inhibitor compounds, thus indicating that this biomarker is involved in chymase activity.01-07-2010
20100003713Methods of Determination of Iron Deficiency and Hemochromatosis - New diagnostic parameters or indexes for detection of absolute iron deficiency, latent iron deficiency, functional iron deficiency, or latent functional iron deficiency have been disclosed. The parameters include a RBC size factor, RSf01-07-2010
20110151500Blood-Platelet Test Method and Blood-Platelet Test Device - A method for testing platelet function, wherein platelet function is tested by allowing anticoagulated blood, to which a weak platelet-activating reagent has been mixed, to pass through a capillary having a platelet-adhesive surface on at least a part of its inner surface, and observing or measuring the behavior of the blood in the capillary.06-23-2011
20110151498APPARATUS AND METHODS FOR CONDUCTING ASSAYS AND HIGH THROUGHPUT SCREENING - The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.06-23-2011
20090317855RECEPTOR-MEDIATED DELIVERY: COMPOSITIONS AND METHODS - Compositions and methods for delivering an agent to a cell comprising a prolactin receptor are provided. Also provided is a method of inhibiting a breast, ovarian or prostate cancer cell, where the method includes a step of contacting the cell with a complex comprising a prolactin receptor ligand linked to at least one of an RNAi-inducing agent, a polynucleotide sequence encoding a polypeptide, an miRNA, a cytotoxic moiety, a chemotherapeutic moiety, a radioactive moiety or a nanoparticle. Methods of detecting a cancer cell expressing a prolactin receptor are also provided.12-24-2009
20090317853METHOD FOR PRODUCING A LAYER ON A MOLDED ARTICLE AND USE THEREOF - A method for producing a layer on a molded article. The method includes providing a formable film. Galvanically catalytically active nuclei are anchored to at least one region of the formable film provided for the layer. The formable film is shaped so as to form the molded article. A galvanic deposition is performed on a surface of the molded article so as to bond the nuclei to form the layer.12-24-2009
20090298111METHOD AND COMPOSITION FOR A PROTEIN TRANSDUCTION TECHNOLOGY AND ITS APPLICATIONS - A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.12-03-2009
20090286273USE OF NEUREGULIN-BETA AS AN INDICATOR AND/OR TARGET - The invention relates, inter alia, to the use of neuregulin-β as a target in a screening method for active compounds, in particular for exerting an influence on changes in calcium concentration which are mediated by glutamate receptors.11-19-2009
20090239255Odontogenic ameloblast-associated protein as a tumor biomarker - Odontogenic ameloblast-associated (ODAM) protein may be used as an effective biomarker in humans for various cancers, especially those of epithelial origin, such as breast, lung, and stomach. The ODAM protein may be detected in cancerous cells. Additionally, the blood of patients with such cancers contains high titers of anti-ODAM antibodies.09-24-2009
20090221023CELL TRAY - A cell tray has a multi-dimensional array of cells in precise, equally spaced wells (cubicles or silos) containing medium of interest. The ordered cell array enables automated processing as well as simultaneous monitoring and analyzing of a large matrix of cells, biological fluids, chemicals and/or solid samples. The invention is an integrated device and is fabricated into substrates similar to microscope slides. The ordered array of cells in precise locations helps in parallel analysis and processing of cells simultaneously. Each cell cubicle or silo in the array is located equidistant from its nearest neighbors in an orthogonal direction. The location of each well can be precisely measured and recorded in an automated processing system. Included in the bottom of each cell well is an optional micro-lens. An array of probes provides parallel cell processing and monitoring capabilities, including microinjection and microscope analysis.09-03-2009
20090215104Bioreactor Device, and Method and System for Fabricating Tissues in the Bioreactor Device - A bioreactor device, and a method and system for fabricating tissues and growing cells and tissues in the bioreactor device, accommodates less than about 1 mL (or less than about 200 μL) of local medium volume but sample sizes of about 100 μL or greater. The bioreactor device includes a bioreactor chamber for containing a sample, where sample growth in response to mechanical, electrical, and biofactor stimulation is monitored through one or more optical ports. Embedded sensors are provided for measuring fluid pressure, pH, temperature, and oxygen tension. The bioreactor device can receive different types of mechanical loadings, including fluid shear, hydrostatic pressure, matrix compression, and clinorotation.08-27-2009
20110256573METHOD AND APPARATUS FOR DETERMINING AT LEAST ONE HEMOGLOBIN RELATED PARAMETER OF A WHOLE BLOOD SAMPLE - A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.10-20-2011
20110256580LC-MFR-MS-Based Method and Apparatus for Screening a New Drug Candidate - The present invention relates to a method for screening a new drug candidate using a liquid chromatography/microfluidic device/mass spectrometry system, and to a liquid chromatography/microfluidic device/mass spectrometry system. The present invention involves the detection of an interaction between molecules on a real-time basis through adjustment of a microreaction between traces of natural material or synthesized new drug candidates and a target material (protein or cell, etc.), thus developing materials for new drug candidates at a lower cost and with high efficiency, while improving quality of life and reducing medical costs. The present invention can be valuably used in increasing new scientific technology through the convergence of nanotechnology, biotechnology, and analytical chemistry technology.10-20-2011
20080293091APPARATUS AND METHODS FOR AUTOMATED DIFFUSION FILTRATION, CULTURING AND PHOTOMETRIC DETECTION AND ENUMERATION OF MICROBIOLOGICAL PARAMETERS IN FLUID SAMPLES - A system providing non-intrusive, automated culturing and photometric detection for analyzing microbiological parameters is described. The system includes a sealed non-intrusive sample cuvette, a housing with an enclosable cover, systems for incubation and photometric detection mounted within the housing. The sample cuvette consists of a clear graduated optically transmitive container with two chambers—a culture chamber and a detection chamber, separated by a permeable membrane wall. The cuvette has an upper part and a lower part of different dimensions. The upper part is bigger in size than the lower part. The sealed top of the cuvette has two fluid inlet/outlet ports for the introduction of the sample into one chamber while when connected to a suitable vacuum device, the second chamber receives the filtered sample through the permeable membrane. The housing has a cuvette holder that is shaped to provide a very snug fit for the cuvette. When placed inside the cuvette holder the bottom of the upper part of the cuvette rests on top of the holder while the bottom part snugly fits inside the holder cavity. The housing with the enclosable cover provides a thermal chamber during simultaneous incubation and photometric enumeration of microbiological parameters. The cuvette holder accommodates a heating element and a temperature sensor. Photometric detection components comprising LEDs and detectors are placed strategically within the holder.11-27-2008
20090075320Method for screening an enhancer or a masker of salty taste - The invention relates to a nucleic acid molecule being represented by a nucleic acid sequence comprising at least three nucleic acid subsequences, each nucleic acid subsequence coding for one of the α, β, and γ subunits of the epithelial sodium channel, and each nucleic acid subsequence being arranged in a head to tail configuration with respect to another. The invention further relates to a cell comprising the nucleic acid sequence of the invention, which is able to express the subunits and to its use in a method for screening a potential modulator compound of the subunits of the epithelial sodium channel.03-19-2009
20110212483Methods and Kits for the Diagnosis of RyR1-Related Diseases - The present invention encompasses methods for diagnosis of calcium channel related diseases (e.g., RyR1-related diseases) comprising contacting lymphocytes isolated from a subject with a calcium channel agonist (e.g., an RyR1 agonist), measuring the adenosine and inosine produced by the lymphocytes; and comparing the measured levels in the sample to the adenosine and inosine levels in a normal control, whereby an increase in the adenosine and inosine levels relative to the control is indicative of a calcium channel related disease (e.g., RyR1-related disease). The invention also encompasses kits for diagnosis of calcium channel-related diseases.09-01-2011
20110212482METHODS AND DEVICES FOR SAMPLE COLLECTION, TREATMENT AND DISPENSING - A device for collecting, treating and dispensing a biological sample is described. Use of the device permits integration of sample collection, sample treatment, and sample dispensing.09-01-2011
20090053754Assay for Low Molecular Weight Heparin - A prothrombin time reagent for determination of low molecular weight heparin in fresh whole blood and in anti-coagulant treated blood is provided. The reagent is composed of recombinant animal tissue factor, and a mixture of synthetic phospholipids, which mixture includes a phosphatidylalcohol. A formulation buffer which includes a sensitivity adjuster is used in formulating the reagent. The recombinant animal tissue factor includes rabbit brain. The synthetic phospholipids of the mixture include palmitoyloleoylphosphatidylcholine (POPC), palmitoyloleoyl-phosphatidylserine (POPS), and a phosphatidylalcohol. The phosphatidyl alcohol includes dioleoylphosphatidylethanol, dioleoylphosphatidylmethanol, dioleoylphosphatidylpropanol, dioleoylphosphatidylbutanol, and dioleoylphosphatidylinositol. The sensitivity adjuster included in the formulation buffer is γ-Cyclodextrin. The formulated reagent is air-dried and remains stable for at least 3 weeks at 37° C.02-26-2009
20110212481ASSAYS AND METHODS FOR FUSING CELL AGGREGATES TO FORM PROTO-TISSUES - Provided are assays and methods for creating proto-tissues from aggregates of cells. The invention concerns assays and methods useful in tissue engineering and reconstruction techniques, specifically in the formation of macrotissues from microtissues using microtissue pre-culture time as a controlling parameter.09-01-2011
20090029402Assessment of Biological or Chemical Samples - A method for monitoring consumption or release of a gaseous analyte such as oxygen by a liquid sample under investigation includes providing a cuvette (01-29-2009
20110256579Method for Verifying and/or Identifying Hormonally Effective Substances - The invention relates to a method for verifying and/or identifying hormonally effective substances using the pheromone system of yeast, to yeast cells for carrying out the method, and to the use thereof. The method according to the invention comprises the following steps: a) adding yeast culture medium or buffer to a sample which presumably contains a hormonally effective substance, b) thereafter contacting the sample with haploid yeast cells that possess features 1 through 3: 1. the DNA sequence coded for a heterologous hormone receptor is placed under the control of a constitutive promoter, 2. the DNA sequence coded for a yeast pheromone is placed under the control of a promoter that can be regulated by the hormone receptor, 3. a gene that is coded for a receptor for the yeast pheromone is constitutively expressed, wherein the activation of the hormone receptor by the hormonally effective substance leads to the expression and secretion of the yeast pheromone, and c) the physiological and/or morphological changes of haploid yeast cells are detected.10-20-2011
20110256578Detection method using nanoaggregate-embedded beads and system thereof - The invention discloses a detection method using nanoaggregate-embedded beads and system thereof, which are characterized in that the nanoaggregate of Raman dye and metal nanoparticles is coated by an inorganic oxide to form a nanoaggregate-embedded bead, and which is then conjugated with a probe molecule to form a sensor bead. The Raman spectra of the product formed by binding of the sensor bead and an analyte in a sample is detected for determining whether the analyte exists in the sample. In embodiment, the pH of the solution of metal nanoparticles is controlled to keep at 10, and the concentration of the Raman dye is controlled to keep between 1×1010-20-2011
20110256577METHOD FOR SENSING A BIOCHEMICAL AND/OR BIOMECHANICAL PROCESS OF A BIOLOGICAL MATERIAL AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS - A method for sensing a biochemical and/or biomechanical process of a biological material, comprising the steps of: disposing at least a part of a microresonator into the biological material; and before, during, or after disposing the part of the microresonator into the biological material, sensing the process of the biological material by analysis of one or more optical cavity modes of the microresonator.10-20-2011
20110256575DIGITAL SAMPLING APPARATUS AND METHODS FOR SORTING PARTICLES - A system and method for sorting a mixture of stained particles including a digital signal processor for analyzing and classifying the digital information generated from the particles and providing a sorting signal to a sorting system as a function of the analyzed and classified digital information.10-20-2011
20110256572BIOLOGICAL SAMPLE DISCRIMINATION APPARATUS, BIOLOGICAL SAMPLE DISCRIMINATION METHOD, AND BIOLOGICAL SAMPLE DISCRIMINATION PLATE - A plate on which a channel pattern is formed, which channel pattern includes a first channel into which a buffer agent is injected, and a second channel having, in a portion thereof, a quantification part that has a portion common to the first channel and holds a predetermined amount of a biological sample, the biological sample being injected into the channel including the quantification part, and the plate is rotated at a high speed by a filling unit to fill the buffer agent in the first channel, and thereafter, the second channel is pressurized by a discrimination unit to fill the biological sample in the second channel, and simultaneously, a predetermined amount of the biological sample is added into the buffer agent. Therefore, when performing discrimination of the biological sample, a discrimination result can be obtained accurately in a short time without the necessity of complicated preparation works.10-20-2011
20080268490METHODS FOR IN VITRO GROWTH OF HAIR FOLLICLES - The invention is directed to a chemically defined animal cell culture media, and methods for preparing such a medium, wherein the media are suitable for culturing epidermal cells, preferably human epidermal cells, including cells of the hair follicle. The invention further provides for methods of culturing epidermal cells, hair follicles, and skin explants in the media as well as uses of the cell cultures and explant cultures in screening assays.10-30-2008
20080268492Methods for Determining Optimal Techniques for Vitrification of Isolated Cells - A method to optimize a vitrification procedure for suspended cells uses factors such as the physical properties of solutions, the cell permeability to water and permeable cryoprotectants, and the osmotic tolerance of the cells to identify a method to minimize several stresses associated with vitrification procedures.10-30-2008
20080268491INSTRUMENTS FOR DIRECT DETECTION OF FREE METALS IN FLUIDS AND METHODS TO DIAGNOSE METAL-RELATED DISEASES AND DETERMINE PHARMACOLOGIC DOSING REGIMENS - A method and apparatus for measuring the level of metal in a biological sample can employ a current measuring device. It preferably includes a display for displaying the level of metal, and preferably free metal, in the sample. It can use a test strip interfaced to a potentiostat. The test strip preferably includes layers that separate a part of the sample which contains the free metal. Electrodes enable measurement of free metal in the separated part of the sample.10-30-2008
20080268489Calcium Ion Leakage Inhibitors - A polypeptide exhibiting the effect of inhibiting Ca10-30-2008
20120064565System Setup For Biological Methane Potential Test - A system setup is disclosed for carrying out a biological methane potential test on a biosample and a system setup is disclosed for measuring biomethane gas in a biogas flow. In at least one embodiment, the system setups include at least one container for chemical irreversible fixation of other gases than biomethane gas, the other gases including at least CO03-15-2012
20120064561ACTIVITY OF FE-S CLUSTER REQUIRING PROTEINS - The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.03-15-2012
20080213817Enhancement of Th2-Dependent and Inflammatory Response - The present invention relates to altering the levels of Th2 cytokine production, and in particular, biasing the cytokine expression profile towards Th2 cytokine production through mitogen-activated protein kinase/ERK kinase kinase 1 (MEKK1), the screening of agents that increase Th2 cytokine production, and the treatment of Th1 associated autoimmune diseases in vivo. In one embodiment, the present invention relates to agents including but not limited to reducing the activity of MEKK1, leading to increased levels of Th2 cytokine production.09-04-2008
20120231488FLUID SAMPLE COLLECTION DEVICE - Disclosed herein is a sample collection device for an aqueous fluid, such as whole blood, serum, plasma and urine. The device comprises a cartridge body defining an elongate sample collection passage that has open ends. The passage is arranged to draw the fluid into the passage by capillary action. The passage is provided along a portion of its length with a sample metering stop in the form of a hydrophobic coating arranged to prevent flow of the fluid by capillary action thereacross. A sample receiving portion of the passage extending between a collection end and the metering stop is non-linear, and preferably defines a pair of straight limbs connected by a bend. By providing a non-linear passage in this way, the maximum gravitational force which can act on the collected sample is reduced as compared to a conventional linear passage, thereby reducing the tendency of the sample to leak from the device and potentially avoiding the need for one or both ends of the passage to be sealed. The sample receiving portion of the passage may be provided with a hydrophilic coating to enhance the capillary action. A particularly suitable hydrophilic coating for a whole blood sample collection device is heparin, which may also serve as an anticoagulant for the blood.09-13-2012
20100136598NOVEL MESENCHYMAL PROGENITOR CELLS DERIVED FROM HUMAN BLASTOCYST-DERIVED STEM CELLS - Described herein is a mesenchymal human progenitor (hBS-MP) cell population derived from human blastocyst-derived stem (hBS) cells and a method to obtain the progenitor cell population in which is eliminated the need of co-culture steps, cell sorting, manual selection, and transfections. Also, the use of the hBS-MP cells in drug discovery and specifically for toxicity testings as well as for therapeutic use is made possible.06-03-2010
20120202240Method for Predicting the likelihood of an Onset of an Inflammation Associated Organ Failure - The present invention relates to a reliable and statistically significant method for predicting the likelihood of an onset of an inflammation associated organ failure from a biological sample of a mammalian subject in vitro, by means of a subject's quantitative metabolomics profile comprising a plurality of endogenous metabolites, and comparing it with a quantitative reference metabolomics profile of a plurality of endogenous organ failure predictive target metabolites in order to predict whether the subject is likely or unlikely to develop an organ failure. Furthermore, the invention relates to the usefulness of endogenous organ failure predictive target metabolites in such a method.08-09-2012
20120202239MATERIALS, MONITORING, AND CONTROLLING TISSUE GROWTH USING MAGNETIC NANOPARTICLES - Systems and method for releasing a biological factor in a tissue or organ are disclosed. The system includes one or more nanoparticles distributed in the tissue or organ, the nanoparticles including the biological factor; and a magnetic field generator configured to generate a magnetic field at a first frequency and to apply to the tissue or organ the magnetic field at the first frequency thereby causing at least some of the biological factor to be released from each of the nanoparticles into the tissue or organ.08-09-2012
20120202238DETERMINING CONDITIONS IN CENTRIFUGED BLOOD USING MEASURED PRESSURE - A method for determining a condition in a blood sample includes: providing a sample of blood; providing a metering probe having a pump for aspirating and dispensing; inserting the metering probe a selected distance into the blood sample; measuring the pressure between the sample and pump during sample aspiration or sample dispense; comparing the measured pressure with a reference value; and signaling the presence or absence of the condition. A method for confirming or detecting the presence of a selected layer of blood component in a centrifuged blood sample includes: measuring a pressure of a suspected selected layer in a metering probe during aspiration or dispense; comparing the measured pressure with a reference value, wherein if the measured pressure and the reference value are substantially identical then the selected layer of the blood component is confirmed. In a preferred embodiment the reference value is a pre-selected pressure range.08-09-2012
20110020857Metabonomic Methods to Assess Health of Skin - The present invention relates to methods of assessing the health of skin. Biomarkers are used to evaluate skin samples. Using metabonomics approaches, samples taken from different skin sites or at different times during a treatment are used to diagnose skin conditions or to appraise various skin treatments for efficacy.01-27-2011
20110020855Method and apparatus for performing cytometry - Embodiments of the present disclosure generally include a method and apparatus for performing cytometry. Specifically, embodiments of the invention comprise apparatus for providing a sample fluid to a cytometry system comprising a cytometry chip and a holder. The cytometry chip is for channeling a sample fluid from a sample fluid port to an output channel. The holder is configured to retain the cytometry chip within the holder. The holder comprises an interface between the cytometry chip and at least one of a source of the sample fluid, a source of a sheath fluid or a source of electric charge. Embodiments of a method comprise using the apparatus to provide a sample fluid to a cytometry system.01-27-2011
20110053208BIOLOGICAL SAMPLE IDENTIFICATION SYSTEM - The present invention contemplates a handling device for a biological specimen. The handling device includes a substrate with at least one crease that divides the substrate into a handle portion and a receiving portion that includes an aperture having a perimeter that is configured so that it receives a container having a cover that includes a biological specimen for testing, while resisting pull-through of the container relative to the substrate.03-03-2011
20110053207SYSTEM AND METHOD FOR IN VITRO BLOOD VESSEL MODELING - The present invention provides an in vitro blood vessel model for investigation of drug induced vascular injury and other vascular pathologies. The in vitro blood vessel model provides two channels separated by a porous membrane that is coated on one side by an endothelial cell layer and is coated on the other side by a smooth muscle cell layer, wherein said model is susceptible to the extravasation of red blood cells across said porous membrane due to drug induced vascular injury.03-03-2011
20110053206METHOD OF USING LIGAND-FREE LYSING AGENT IN HEMOGLOBIN ANALYSIS - Lysing agents that are free of ligands, including cyanide, for binding hemoglobin for hematology analyzers. The ligand-free lysing agents achieve accurate quantification of hemoglobin parameters, thereby replacing existing lysing agents for analysis of hemoglobin.03-03-2011
20110053209USE OF AN IMMUNOREGULATORY NK CELL POPULATION FOR MONITORING THE EFFICACY OF ANTI-IL-2R ANTIBODIES IN MULTIPLE SCLEROSIS PATIENTS - The use of CD56bright NK cell counts as a biomarker for the efficacy of anti-IL-2R antibody treatment in patients diagnosed with multiple sclerosis.03-03-2011
20110053205Composition and Methods for Expressing Reporter Molecules in Mammalian Cells - Disclosed herein is a novel system and methods for expressing exogenous genes, such as genes encoding fluorescent proteins, in mammalian cells. In one embodiment of this system and methods, a gene essential for viral infectivity or replication in cell culture is deleted or inactivated in the genome of a non-mammalian DNA virus. The exogenous gene operably linked to a mammalian promoter is then inserted into the non-replicative non-mammalian DNA virus. The non-replicative virus is propagated in a host cell that expresses in trans the deleted or inactivated gene or a functional homolog.03-03-2011
20110053204USE OF AN ADAPTIVE CHEMICALLY REACTIVE PLASMA FOR PRODUCTION OF MICROBIAL DERIVED MATERIALS - A relatively lower value carbonaceous feedstock can be converted into a relatively higher value chemical product, by introducing the relatively lower value carbonaceous feedstock into an inductively coupled plasma (ICP) torch under conditions selected to generate a synthetic gas mixture having a tailored composition, and then introducing the synthetic gas mixture into a microbial digester configured to convert the synthetic gas mixture into the product. Significantly, the composition of the synthetic gas mixture produced by the ICP torch can be quickly modified to correspond to an optimal quality and quantity required by the digester, such that if the quantity of composition of the synthetic gas mixture being provided does not meet the needs of the digester, the quantity and stoichiometric ratio can be quickly varied. This enables greater efficiency to be achieved as compared to systems where the quality and quantity of the synthetic gas mixture cannot be easily changed.03-03-2011
20110053203COPOLYMER ASSAY - The present invention provides methods and compositions for evaluating one or more properties of an amino acid copolymer.03-03-2011
20110053202ANALYTICAL SYSTEM, ANALYTICAL METHOD AND FLOW-PATH STRUCTURE - An analytical system for performing centrifugal analysis on a working fluid with different components includes a uniform-dividing unit with a reduced cross section to divide the working fluid, a separating unit connected to the uniform-dividing unit, and a detecting unit. The detecting unit includes a detection compartment and a constant-quantity region connected to the separating unit through a separation channel. When rotating the uniform-dividing unit, the working fluid located at the uniform-dividing unit is transmitted to and separated by the separating unit, thereby causing one component of the working fluid to be separated from the other component and transmitted to the detection compartment through the constant-quantity region. With constant-quantity region, the detection compartment can be prevented from flushing by the excess of the separated component, and thus yield of product detection can be increased and different assays detection is carried out with a small sample volume at the same time.03-03-2011
20110027818APPARATUS AND METHOD FOR DETECTING ZINC IONS - The present invention provides apparatuses and methods for determining zinc ion concentration in a fluid sample. Some apparatuses of the invention include (i) a light source designed to emit an excitatory light at a first known wavelength that activates a fluorescent emission having a second and different wavelength by at least one selected fluorescent reporter probe that generates a different fluorescent response when bound to zinc ion; (ii) at least one sample-holding component adapted to hold a liquid sample in a pathway of the excitatory light; (iii) a photodetector for measuring fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion within the liquid sample; and (iv) electronic means for creating a data signal that correlates with fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion in the liquid sample to the free zinc ion concentration. Such apparatuses and methods can be used to determine the presence of or the risk of having prostate cancer in a subject.02-03-2011
20100285516CALCIUM FLUX AS A PHARMACOEFFICACY BIOMARKER FOR INHIBITORS OF HISTONE DEACETYLASE - Described herein are methods for using calcium flux as a biomarker to select and predict patients likely to respond to an apoptotic agent as therapy. Further described herein is a method of using calcium flux as a clinical biomarker to determine whether a tumor is sensitive to an HDAC inhibitor.11-11-2010
20100285515FLUORESCENT PROBE - A compound represented by the following general formula (I):11-11-2010
20110256574Microfluidic Continuous Flow Device - A microfluidic continuous flow device comprising a channel which comprises a first and a second area wherein the first area of the channel is a compartment which is defined by partitioning elements and the second area of the channel is a space outside the compartment; wherein through passages which are formed between the partitioning elements are dimensioned such as to retain a biological material and optionally a sustained release composition which can be comprised in the compartment within the compartment; wherein the channel has a first inlet to the compartment through which biological material can be introduced into the compartment; a second inlet for introducing a cultivation medium into a space of the channel arranged outside of the compartment, and an outlet. The present invention further refers to methods of using the devices of the present invention and kits comprising the microfluidic continuous flow devices of the present invention.10-20-2011
20110076712LUBRICIOUS MICROFLUDIC FLOW PATH SYSTEM - A flow cytometer having a nozzle assembly which provides a lubricious flow path through which a plurality of particles pass to be entrained in a flow of liquid to provide a faster method for sorting with increased cell quality.03-31-2011
20110136164CARTRIDGE DEVICE FOR BLOOD ANALYSIS - A cartridge device having a receiving portion for receiving a blood sample and a jack portion for receiving a plug; a stirring device for circulating the blood sample within the receiving portion; and an electrode holder having at least one incorporated electrode wire pair; wherein the electrode holder is attachable to the cell such that one end of the at least one electrode wire pair forms a sensor unit for measuring the electrical impedance between the two electrode wires of the at least one electrode wire pair within the blood sample and that the opposite end of the at least one electrode wire pair forms a plug portion being connectable directly to the plug for an electrical connection of the sensor unit to an analyser.06-09-2011
20110136162Compositions and Methods for Functionalized Patterning of Tissue Engineering Substrates Including Bioprinting Cell-Laden Constructs for Multicompartment Tissue Chambers - The present invention relates to microfluidic systems and methods for monitoring or detecting a change in a characteristic of an input substance. Specifically, the invention relates to a model for in vitro pharmacokinetic study and other pharmaceutical applications, as well as other uses including computing, sensing, filtration, detoxification, production of chemicals and biomolecules, testing cell/tissue behavior, toxicology, drug metabolism, drug screening, drug discovery, and implantation into a subject. The present invention also relates to systems and methods of a microplasm functionalized surface patterning of a substrate. The present invention represents an improvement over existing plasma systems used to modify the surface of a substrate, as the present invention creates surface patterning without the use of a mask, stamp or a chemical treatment.06-09-2011
20100323389FLUORESCENT IMAGING WITH SUBSTITUTED CYANINE DYES - Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared spectral range. The cyanine compounds of Formula I are presented:12-23-2010
20100311101Method to Predict Toxicity Using the Analysis of Dynamic Organelle Behaviour - The present invention provides an in vitro method to predict the effect of an exogenous element on an animal or human organism. This method is based on the analysis of variations of an organelle behaviour represented by at least two characteristics in isolated cells induced by the contact with the exogenous element and the determination of the cluster membership of the exogenous element which is representative of the effect of said element. Analyzed characteristics are selected from the group consisting of the motility, the morphology, the relationship with the cell cytoskeleton and the membrane permeability of said organelle. This method may be used to predict the efficacy or the toxicity of an element, such as a drug.12-09-2010
20100311100APPLANATION SYSTEM FOR EVALUATION OF CELL PRESSURE DEPENDENT FIRMNESS ON LEAVES AND SOFT ORGANS FLAT FACE SEGMENTS - The applanation system on air permeated screen (12-09-2010
20100323387Optimization of Response to Light - Various aspects provide for exposing a substance to light. Certain aspects include exposing a suspension of photosynthetic organisms to sunlight, and may include optimizing exposure to improve photosynthesis conditions. Certain embodiments include controlling an opacity or opacity profile of a suspension of algae and/or diatoms. Optimizing exposure may include maximizing growth rate, maximizing photosynthesis efficiency, maximizing lipid production, minimizing damage, minimizing predator growth, maximizing a capacity to grow in suboptimal media (e.g., polluted water, brackish water, or water having a pH outside of a preferable range), minimizing requirements for nutrients, and other features.12-23-2010
20100323386Method and apparatus to indicate combustor performance for processing chemical/biological contaminated waste - Methods and apparatus are used for monitoring the effectiveness of a heat treatment to inactivate a contaminant in or on common building materials. The temperature is monitored or evaluated by using an internal control having a biological, chemical or electronic sensor. The sensor is bundled in common building materials to provide insulating properties so as to mimic bundles of contaminated building materials being bundled for incineration.12-23-2010
20100323390Method for the Identification of a Metabolic Pathway Family by Means of Positive Selection - The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.12-23-2010
20100330612BIOCHIP FOR ELECTROPHYSIOLOGICAL MEASUREMENTS - The present invention relates to a substrate for measuring the electrophysiological properties of ion-channels located in cell membranes. The substrate is typically used in a screening device providing high-throughput industrialized measurements for studying ionic currents, particularly useful in the screening of drugs acting on the ion-channels found in cell membranes, by providing many parallel simultaneous and independent measurements. The substrate has one or a plurality of individually addressable electrode sites, each comprising one or more individual elongated nanosize electrodes, capable of penetrating the cell membranes during application of cells directly on the substrate, thus providing one or more low resistance contacts to the interior of the cells. This allows for an easy and effective low-cost solution to automated patch clamp measurements in the whole-cell configuration, using both single as well as multi-electrode contacts to each cell. The invention also makes ensemble measurements on multiple cells in parallel possible.12-30-2010
20100330605TEST DEVICE FOR PLATELET AGGREGATION DETECTION - The invention relates to the test device for platelet aggregation detection comprising:—an element (12-30-2010
20100330611Multimodal Spectroscopic Systems and Methods for Classifying Biological Tissue - Multimodal optical spectroscopy systems and methods produce a spectroscopic event to obtain spectroscopic response data from biological tissue and compare the response data with an empirical equation configured to correlate the measured response data and the most probable attributes of the tissue, thus facilitating classification of the tissue based on those attributes for subsequent biopsy or remedial measures as necessary.12-30-2010
20100330609ANALYSIS APPARATUS AND ANALYSIS METHOD - This analysis apparatus includes a transporter transporting the specimens to the first measurement unit and the second measurement unit, and a control portion so controlling the transporter as to transport a first specimen container, stored in the rack, storing a first specimen to the first measurement unit and as to transport a second specimen container, stored in the rack along with the first specimen container, storing a second specimen to the second measurement unit.12-30-2010
20100330606COMPOSITIONS AND METHODS FOR OPTIMIZING DRUG HYDROPHOBICITY AND DRUG DELIVERY TO CELLS - Methods to determine drug hydrophobicity and to quantify changes in drug hydrophobicity that optimize drug function by means of differential scanning calorimetry of an endothermic phase transition of a base protein-based polymer, specifically of an elastic-contractile model protein, to which is attached the drug to be evaluated for its hydrophobicity in terms of the change in Gibbs free energy for hydrophobic association, ΔG12-30-2010
20100330601CONTROL SYSTEM FOR UV LAMPS, AND CHECK SYSTEM FOR DETERMINING THE VIABILITY OF MICROORGANISMS - The invention relates to a control system for a method for controlling at least one UV lamp for treating a liquid such as water, wherein a biosensor is used. In addition, the invention relates to the use of biosensors for detecting or monitoring viable cells. The invention uses one or more viability parameters.12-30-2010
20100330607PHOTOSWITCH-ENABLED ION CHANNEL ASSAY SYSTEM - The present invention provides a system for assaying ion-channels. In some embodiments, the ion-channel assay provides a reversible change to the membrane potential of a target, e.g., a cell, upon exposure to light. In some embodiments, the membrane potential readout is fed back through a control circuit to regulate the excitation intensity of the illumination sources that induce, respectively, hyperpolarizing and depolarizing currents, thereby effecting closed-loop control of the membrane potential.12-30-2010
20100330603PORTABLE MICROBIOLOGICAL TESTING DEVICE FOR GASES - A method and apparatus for microbiological testing of biogas and other gaseous streams in which a liquid bacteria growth medium disposed within a lower region of a portable sampling vessel is contacted with a gas sample of interest. The gas sample is passed through a hydrophobic filter element which retains microorganisms in the gas sample. The sampling vessel is then inverted, thereby submerging the hydrophobic filter element in the liquid bacteria growth medium. The sampling vessel is incubated for a predetermined period of time at a predetermined temperature following which a presence or absence of microorganisms is determined based on turbidity and/or color of the liquid bacteria growth medium.12-30-2010
20110256576RECOVERY AND DETECTION OF MICROORGANISMS FROM MIXED CELLULOSE ESTER FILTRATION SUPPORTS BY SEQUENTIAL TREATMENT WITH METHANOL AND ACETONE - The present invention is directed to the recovery of bacteria and microparasites, particularly 10-20-2011
20110256581CELL SEPARATION TECHNIQUE - In a first aspect, the present invention provides a method of removing non-viable cells from a cell population, said method comprising contacting a cell population with a compound under conditions suitable to permit binding between the compound and any non-viable cells present in the cell culture and removing at least some of the compound from said cell population. The invention further provides compositions suitable for use in these methods, further uses of such compositions and various other systems and kits.10-20-2011
20090311738METHOD AND SYSTEM FOR DETERMINING PROPERTIES OF BIOLOGICAL CELLS12-17-2009
20110086376In-Vivo Platelet Function Test By Online Bleeding Volume Measurement - A method for remotely determining a patient's excessive bleeding tendency and a patient's resistance to blood thinning medication is disclosed. An incision is made in the patient's forearm. Blood oozing out of the incision is absorbed into a blotter paper until the bleeding stops. Blotches of blood formed on the blotter paper are captured as an image and sent to a service provider who calculates a value associated with the bleeding volume of the patient. The service provider retransmits a value associated with the bleeding volume back to the medical professional. To determine the resistance to blood thinning medication, one incision is made in the patient prior to administration of blood thinning medication. Blood oozing out of the incision is collected on blotter paper until the patient stops bleeding. A second incision is made in the patient. A second set of blotter paper is used to collect the blood oozing out of the incision until the bleeding stops. Both sets of blotter paper are sent to a service provider to calculate a value associated with the difference in bleeding volume. The service provider then retransmits the value associated with the difference in bleeding volume to the medical professional.04-14-2011
20100112625METHOD AND APPARATUS FOR PROCESSING TISSUE SAMPLES USING A SENSOR - An apparatus and a method for processing tissue samples are described. The apparatus comprises: at least one retort for accommodating tissue samples; at least one container for storing alcohol or xylene; a valve adapted to connect the at least one retort with the at least one container depending on an operating position of the valve; and at least a first sensor that is arranged in flow direction between the container and the retort for measuring a value of a parameter that represents a purity level of the alcohol or xylene; wherein the first sensor and the valve are configured to replace the alcohol or xylene depending on the value of the parameter that represents the purity level. The method comprises for the alcohol or xylene conducting between the container and the retort; automatically measuring the purity level and replacing depending on the purity level.05-06-2010
20100184118BLOOD COLLECTOR DEVICE AND BLOOD ANALYSIS PROCEDURE - The present invention provides an improved arterialized earlobe blood collection device, and an improved process for blood analysis. The device and the process of the invention can be used in many unsual and/or risky situations, including collecting arterialized blood in space missions under microgravity environment and within ambulances or the like. The process of the present invention enables easier and faster blood analysis, since the blood collector device of the invention is coupled with an analyzing apparatus so as no blood or needle manipulation is required.07-22-2010
20100184117METHOD AND SYSTEM FOR CHARACTERIZING A PIGMENTED BIOLOGICAL TISSUE - Method for determining the content of a non-fluorescent chromophorous first compound, in a biological tissue including a fluorescent chromophorous second compound, the method includes at least one iteration of the following operations: 07-22-2010
20100184115Cell-Impedance Sensors - Methods and apparatus for designing and measuring a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. The method of designing a cell-electrode impedance sensor comprises: determining a cell free cell-electrode impedance and a cell-covered cell-electrode impedance; obtaining a sensor sensitivity of the cell-electrode impedance measurement system; and choosing one or more design parameters of the cell-electrode impedance sensor to maximize the sensor sensitivity. When the frequency of AC signal between electrodes ranges from 10 kHz to 40 kHz, the sensitivity of the sensor is maximized.07-22-2010
20100184119LASER MEDIATED SECTIONING AND TRANSFER OF CELL COLONIES - Disclosed herein are methods and devices for sectioning cell colonies. Also disclosed are methods of purifying cell colonies. A method of sectioning cell colonies can include providing a cell colony on a culture plate comprising a known thickness; positioning a bottom of the culture plate using automated focus technology; and sectioning the cell colony into one or more pieces using a pattern of laser cutting lines. Devices for performing the method are also disclosed.07-22-2010
20100184114METHOD FOR SCREENING ALLERGIC DISEASE-RELATED MOLECULE AND CELL LINES TO BE USED IN THE METHOD - An allergic disease-related substance is screened from candidate substances based on the reactivity to a plurality of cell lines, which are basophil-like or mast cell-like cell lines derived from the same spleen tissue and have different sensitivities to allergic cytokines. Further, an allergic disease-related substance is screened from candidate substances using at least one basophil-like or mast cell-like cell lines selected from the group consisting of R cell (Deposit No. FERM BP-10918), N 62.5 cell (Deposit No. FERM BP-10919) and RCCM cell (Deposit No. FERM BP-10920).07-22-2010
20100184113CONTROLLING OSTEOGENESIS BY INHIBITION OF OSTEOGENIC SUPPRESSORS - The present invention provides methods of screening for agents that inhibit the activity or expression of one or more polypeptides that contribute to the suppression of osteogenesis. The invention also provides methods of inducing osteogenesis in a cell by administering to the cell an agent that inhibits one or more polypeptides that contribute to the suppression of osteogenesis.07-22-2010
20110111447ONE TO ONE IDENTIFICATION SYSTEM - The present invention relates to a system arranged for assisting secure handling of cells in order to minimize the risk of handling mistakes resulting in mix-up of cell samples. The system allows for one to one identification of cells during a whole culturing period. This is obtained by providing a culturing system with loading/exit control means which ensures that only one device is capable of being loaded/removed at a time.05-12-2011
20100216182Cyanine-Based Probe\Tag-Peptide Pair Fluorescence Protein Imaging and Fluorescence Protein Imaging Methods - A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl08-26-2010
20100062472BIOMARKERS FOR DIAGNOSING MULTIPLE SCLEROSIS, AND METHODS THEREOF - The present invention describes methods for the diagnosis and differential diagnosis of the different forms of multiple sclerosis The methods measure the intensities of specific small molecules called metabolites in samples from patients with clinically diagnosed relapsmg-remittmg or primary-progressive forms of multiple sclerosis and compare these intensities to the intensities observed in a population of healthy individuals, thus identifying markers of multiple sclerosis A method is also provided for the differential diagnosis of subjects afflicted with relapsing-renitting multiple sclerosis from secondary-progressive multiple sclerosis.03-11-2010
20100159497Binned Micro-Vessel Density Methods and Apparatus - In one aspect, a method of obtaining micro-vessel density (MVD) measurements from an image of biological vasculature containing a plurality of vessels is provided. The method comprises acts of analyzing a region of interest of the image for each of a plurality of bins, each of the plurality of bins associated with a predetermined range of vessel sizes, the act of analyzing the region of interest including determining which of the plurality of bins that portions of any vessel subject matter identified in the region of interest belong based on a size associated with the respective portions of the vessel subject matter, and associating each portion of the vessel subject matter with the corresponding one of the plurality of bins to which the portion belongs, and computing at least one measurement for each of the plurality of bins, the at least one measurement related to the MVD of the portions of vessel subject matter associated with the respective bin.06-24-2010
20100159503ENHANCED PROCESSES FOR DRUG TESTING AND SCREENING USING TISSUE SAMPLES - A method for testing human tissue in a testing system is more effective than conventional cell culture systems and functions by treating the human tissue slice system's samples with at least one compound and observing the effect on the human tissue slices resident therein, or cells, tissue samples or other derivatives from the testing process.06-24-2010
20100159502METHOD AND APPARATUS FOR ENVIRONMENTAL MONITORING AND BIOPROSPECTING - A method for environmental monitoring and bioprospecting includes the steps of: (a) utilizing a testing device having: (i) a container having a fluid inlet and outlet, (ii) a plurality of capillary microcosms situated within the container, each of these capillaries having an inlet and outlet that are configured so as to allow for fluid flow through the capillaries, each of these capillaries further having a means for covering its inlet and outlet so as to prevent flow through the capillary, (iii) a pump connected to the container outlet, the pump being configured so as to draw fluid from the surrounding environment into the container's inlet and through the capillaries, (iv) connected to the outlet of the container, a means for collecting the flow through the container, and (v) a check valve connected downstream of the container to prevent the backflow of fluid into the container, (b) adding specified test substances to the device's capillaries, wherein these substances are to be analyzed for their ability to accelerate a specified biotransformation process in the subject environment, (c) locating this device in this environment and opening the capillary covering means so as to allow fluid from the surrounding environment to flow though the container and capillaries, (d) leaving the device in situ for a temporal duration sufficient to incubate phenomena occurring within the capillary microcosms, (e) retrieving the testing device, and (f) analyzing phenomena occurring with the capillary microcosms using automated analysis schemes and commercially available robotics.06-24-2010
20100159496EVALUATION METHOD FOR PHOTOSYNTHESIS SAMPLE, EVALUATION SYSTEM FOR PHOTOSYNTHESIS SAMPLE, AND EVALUATION PROGRAM FOR PHOTOSYNTHESIS SAMPLE - An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample.06-24-2010
20100159500Method for determining the haemolysis of a blood sample and device - The invention relates to a method for determining the haemolysis of a blood sample during which the haemolysis progress is determined, wherein the method comprises the steps of irradiating measuring light which is radiated from a measuring light source on the blood sample during haemolysis; detecting measuring light values at several measuring points of time for measuring light transmitted through and/or reflected by the blood sample by a detector device; comparing several of the measuring light values for different measuring points of time and determining a measure for the haemolysis progress by an evaluation device by forming a time-dependent course for the measuring light values from the detected measuring light values, and determining for the time-dependent course at least section-wise a gradient in an assigned measuring curve as a comparative measure for the comparison of the measuring light values at the different measuring points of time; and determining the conclusion of the haemolysis when the gradient within a selectable measurement accuracy, after a measuring period in which the gradient is different from zero, falls to a minimum going down to zero. Another aspect of the invention relates to a device for the determination of the haemolysis of a blood sample.06-24-2010
20100120076METHOD FOR ANTENATAL ESTIMATION OF RISK OF ANEUPLOIDY - The present invention relates to a system and a method for evaluating the risk of carrying a fetus with genetic anomalies such as aneuploidy and in particular, to such a system and method where a screening system and method is provided to identify fetus' having trisomy-21 (Down's syndrome) with the use of biochemical marker concentrations evaluated from the maternal blood serum.05-13-2010
20100120079Trans-Differentiation And Re-Differentiation Of Somatic Cells And Production Of Cells For Cell Therapies - The invention provides a method for effecting the trans-differentiation of a somatic cell, i.e., the conversion of a somatic cell of one cell type into a somatic cell of a different cell type. The method is practiced by culturing a somatic cell in the presence of at least one agent selected from the group consisting of (a) cytoskeletal inhibitors and (b) inhibitors of acetylation, and (c) inhibitors of methylation, and also culturing the cell in the presence of agents or conditions that induce differentiation to a different cell type. The method is useful for producing histocompatible cells for cell therapy.05-13-2010
20120208227APPARATUS AND METHOD FOR PROCESSING CELL CULTURE DATA - One embodiment of the invention provides a method of processing cell culture data. The data comprises results from a large number of samples, the results being obtained by performing multiple stages of cell culture in succession on each sample. Each stage represents a cell culture treatment having a particular set of conditions, such that each sample follows a protocol specified by the identity and order of the treatments applied to the cell culture. The method includes specifying a subset of the samples that yielded a desired cell culture outcome. The method further includes performing a computer-implemented analysis of the results from the samples in the subset to produce an ordering or grouping for the results. The ordering or grouping helps to identify one or more protocols that are effective for obtaining the desired cell culture outcome. The analysis for producing the ordering or grouping utilises information on similarities between different protocols.08-16-2012
20120208228Pipelining Assembly for a Blood Analyzing Instrument - A pipelining assembly for use in a blood analyzing instrument, methods for performing parallel pipelining functions, and methods for processing a plurality of prepared blood samples through a blood analyzing instrument. The pipelining assembly presented generally includes a first sample preparation chamber, a first queuing chamber in fluid communication with the first sample preparation chamber, and a first control valve between the first sample preparation chamber and the first queuing chamber. The pipelining assembly further includes a second sample preparation chamber, a second queuing chamber in fluid communication with the second sample preparation chamber, and a second control valve between the second sample preparation chamber and the second queuing chamber. An analysis chamber is provided to receive first and second prepared blood samples from the in first and second queuing chambers. The presented methods include steps for repeated processing of prepared blood samples through the blood analyzing instrument.08-16-2012
20110045523Optical Nanosensors Comprising Photoluminescent Nanostructures - Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described. Generally, the nanosensors comprise a photoluminescent nanostructure and a polymer that interacts with the photoluminescent nanostructure. In some cases, the interaction between the polymer and the nanostructure can be non-covalent (e.g., via van der Waals interactions). The nanosensors comprising a polymer and a photoluminescent nanostructure may be particularly useful in determining the presence and/or concentration of relatively small molecules, in some embodiments. In addition, in some instances the nanosensors may be capable of determining relatively low concentrations of analytes, in some cases determining as little as a single molecule. In some embodiments, the interaction between the analyte and the nanosensor (e.g., between the analyte and the photoluminescent nanostructure) can be reversible, which may allow, for example, for the reuse of a nanosensor after it has been exposed to an analyte.02-24-2011
20090203064METHOD AND DEVICE FOR MONITORING PLATELET FUNCTION - The invention provides a method of monitoring platelet function in a mammal by passing blood removed from the body of the mammal through a passageway to contact an obstruction or irregularity in the passageway to generate a platelet mass in the passageway, and monitoring the flow or composition of the blood in the passageway to detect the platelet mass. The flow and composition change in response to the formation of a platelet mass in the passageway. Devices, articles, and kits for performing the methods are also disclosed.08-13-2009
20090203063Droplet-based cell culture and cell assays using digital microfluidics - We introduce a new method for implementing cell-based assays and long-term cell culture. The method is based on digital microfluidics (DMF) which is used to actuate nanoliter droplets of reagents and cells on a planar array of electrodes. DMF method is sutable for assaying and culturing both cells in suspension and cells grown on surface (adherent cells). This method is advantageous for cell culture and assays due to the automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays and cell culture on the microscale.08-13-2009
20090203062Method For Identifying And/Or Quantifying Anti-Infective Compounds - The present invention relates to a method for identifying and/or quantifying an anti-infective compound. Furthermore it relates to a method for identifying and/or quantifying a mutation in a virulence factor of a pathogen of a host organism that reduces the virulence of the pathogen to a unicellular test organism.08-13-2009
20090197296Optical Indicator for Detecting Bacterial Pathogens - A clinical testing assay device that can differentiate bacterial from viral infections is described. The assay device has a sample contact zone with an absorbent pad on which a test sample is deposited and a detection zone with a colorant indicator that is sensitive to bacteria cells. The colorant indicator changes color when exposed to a bacteria sample. The color change signal can manifest relatively quickly, usually within a few minutes, and with an intensity correlative to the concentration of bacteria in a test sample. A method of use is also provided.08-06-2009
20090197297Molecularly Imprinted Polymer Sensor Device - A molecularly imprinted polymer sensor device for detecting the presence of a taggant molecular structure in a fluid is disclosed. The molecularly imprinted polymer sensor device comprises a molecularly imprinted crosslinked polymer having a crosslinked core and a plurality of polymer arms attached to the core, wherein the core has molecular sized cavities adapted to selectively receive and bind displacement molecules having the taggant molecular structure and a colorimetric indicator, said displacement molecule being selectively removed from the molecularly imprinted crosslinked polymer and replaced with the taggant molecular structure upon exposure to the fluid containing the taggant molecular structure therein, thereby indicating the presence of the taggant molecular structure in the fluid.08-06-2009
20090176266FUNCTIONAL METHOD TO IDENTIFY TASTANTS - Provided are functional methods using the T1R2 monomer of the T1R2/T1R3 sweet receptor to identify agonists and modulators of the sweet taste response.07-09-2009
20090176265METHOD OF ASSESSING BIOLOGICAL TEST SPECIMEN - A method of assessing a biological test specimen includes the steps of detecting an acoustic emission produced by the biological test specimen and comparing the detected acoustic emission to a compilation of acoustic emissions produced by known biological sources. The method further includes the monitoring of various attributes of the test specimen.07-09-2009
20110189722METHOD FOR PREPARING CELL STANDARD - The present invention relates to a method for preparing cell samples for histological examination and, more particularly, a method for preparing a cell sample as a standard, or control, for use in the fields of cytology and histology.08-04-2011
20100190201Methods - The invention relates to a method of detecting an altered behaviour in a population of cells, said method comprising determining at least one of the following characteristics of the population of cells; (i) the proportion of stem cells, proliferating cells and differentiated cells in said cell population; or (ii) the size of stem cell clusters in said cell population; or (iii) the separation of stem cell clusters in said cell population; and comparing said at least one characteristic to a reference value, wherein a difference between the determined value and the reference value indicates an altered behaviour in said population of cells. Preferably the cells are mammalian, more preferably human epithelial cells, more preferably human epidermal cells.07-29-2010
20100190197NESTED PERMEABLE SUPPORT DEVICE AND METHOD FOR USING THE NESTED PERMEABLE SUPPORT DEVICE - A nested permeable support device is described herein that can be used to perform various experiments to test new therapeutic compounds (new chemical entities). In one application, the nested permeable support device is used to perform a first pass assay to determine the bioavailability of a new chemical entity following absorption through the digestive tract and metabolism by the liver.07-29-2010
20100190203CHEMICAL AMENDMENTS FOR THE STIMULATION OF BIOGENIC GAS GENERATION IN DEPOSITS OF CARBONACEOUS MATERIAL - Methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content are described. The methods may include accessing a consortium of microorganisms in a geologic formation that includes a carbonaceous material. They may also include providing hydrogen and one or more phosphorous compounds to the microorganisms. The combination of the hydrogen and phosphorous compounds stimulates the consortium to metabolize the carbonaceous material into the metabolic product with enhanced hydrogen content. Also, methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content by providing a carboxylate compound, such as acetate, to a consortium of microorganisms is described. The carboxylate compound stimulates the consortium to metabolize carbonaceous material in the formation into the metabolic product with enhanced hydrogen content.07-29-2010
20100190198ASYMMETRIC SYSTEMS - Among other things, a combination comprises interaction with a system that has a perturbation. In such perturbed system, a non-directional input is applied to a first variable of the system. Based on an asymmetry of the perturbed system, a directional effect is achieved in a second variable of the system, the first and second variables comprising a conjugate pair of variables. At least one of the following pertains: the interaction occurs other than by an apparatus and other than in a way that actually achieves the directional effect, or the conjugate pair is other than position and momentum, or the input or the asymmetry are in a dimension other than spatial coordinates, or the directional effect is other than translational motion and other than rotary motion.07-29-2010
20120309045Composite Probes and Use Thereof in Super Resolution Methods - Composite probes for super resolution optical techniques using super resolution via transiently activated quenchers (STAQ) include a donor moiety and an acceptor moiety joined by a linker, wherein the acceptor moiety, when excited by incident radiation, is excited to a state which, for example, absorbs in the donor emission region, such that the acceptor moiety in its excited state quenches at least a portion of the donor moiety emission. Other transiently activated quenching mechanisms and moieties could accomplish the same task by reducing donor population. Also disclosed are methods for irradiating a selected region of a target material including the composite probe, wherein the composite probe enables improved resolution by point spread function modification and/or nanoscale chemical reactions.12-06-2012
20120309046METHOD AND DEVICE FOR DETERMINING THE VIABILITY OF A PLANT SAMPLE - A method for determining the viability of a plant sample includes providing a viability detection device containing a solid or semisolid culture medium suitable for the nutritional requirements of a plant sample, wherein the culture medium has a starch supplement; growing the plant tissue in the viability detection device from the previous step; removing the plant tissue sample from the viability detection device; and revealing the viability detection device.12-06-2012
20120309044METHOD AND SYSTEM FOR USE OF TREATMENT LIQUIDS IN AN APPARATUS FOR STAINING OF TISSUE SPECIMENS ON MICROSCOPE SLIDES - A method and a system for use of treatment liquids in an apparatus for staining of tissue specimens on microscope slides, wherein the apparatus comprises a plurality of vessels (12-06-2012
20110189720AUTOMATED BIOLOGICAL SAMPLE COLLECTION SYSTEM AND METHOD - An automated biological sample collection system and method are disclosed. The system comprises an incubator, an applicator and a sampler. The incubator is configured to control the environment of a plurality of eggs, the applicator is configured to deliver exogenous material to the plurality of eggs and at least one sampler is configured to collect samples from the plurality of eggs, invasively or non-invasively.08-04-2011
20110189725Microbiological Analysis Assembly And Method - The assembly comprises a filtration unit (08-04-2011
20110189724System for testing biological and chemical agents using two or more cartridges simulating systems in the body - The present invention relates to joining two or more cartridges containing cell or tissue cultures from different organs found in the human body in fluid connection. It includes a vitro method and system for testing chemicals and agents as they pass through organs other than the brain or combinations of body systems including the brain. It greatly enhances the ability for researchers to test a proposed chemical or agent on human cells and tissue prior to testing it in vivo. Often, it is found that chemicals or agents have different reactions when tested on a single organ in vitro or in animals, than it does in the human body.08-04-2011
20110189723CELL DETECTION METHOD, AND MICROARRAY CHIP FOR USE IN THE METHOD - Disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell. Specifically disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell, which comprises the following steps (1) and (2): (1) retaining cells contained in the sample on a microarray chip which comprises multiple microchambers, wherein each of the microchambers can contain multiple cells; and (2) confirming the presence or absence of the specific cell in the cells retained on the microarray chip.08-04-2011
20110189721INTERACTIVE TRANSPARENT INDIVIDUAL CELLS BIOCHIP PROCESSOR - An Interactive Transparent Individual Cells Biochip Processor (ITICBP) device is described, which is useful for assessing a single, individual living cell at identifiable location or assessing group of cells each at identifiable location.08-04-2011
20110189719METHODS OF GENERATING PATTERNED SOFT SUBSTRATES AND USES THEREOF - The present invention provides methods of generating and devices of patterned soft substrates, on which cells may be seeded, as well as methods of using these substrates. Devices containing these patterned soft substrates are also provided.08-04-2011
20110189718Systems And Methods For Segregating Mixed Material Streams - The invention relates to methods and systems of detecting useful material in a mixed solid, liquid and/or gaseous material stream. The methods include defining a value range requirement for at least one parameter of interest of useful material to be selected from the material stream, passing the material stream through at least one detector adapted to measure the parameter of interest of the material stream, and separating the material stream into useful material and residue based on the measured parameter. The systems comprise at least one detector adapted to measure a parameter of interest of the material stream passing therethrough; and at least one separator for separating the material stream into useful material and residue based on the measured parameter after passing through the detector. The system may further comprise treaters, processors, and controllers.08-04-2011
20110189717MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY - The invention relates to recombinant expression of a taxadiene synthase enzyme and a geranylgeranyl diphosphate synthase (GGPPS) enzyme in cells and the production of terpenoids.08-04-2011
20110189715MEASURING MULTI-ANALYTE SAMPLES USING AN IN-LINE FLOW CELL - Methods and systems for analyzing ratios of analytes within a flowing sample are provided. The flowing sample can be processed in real-time to determine a time interval over which a predetermined amount of a group of analytes passes by a fixed point in a flow cell. The predetermined amount can be routed to a sample container for future processing. The sample can comprise diluted blood and the analytes can comprise a component of hemoglobin, such as A1c, and the total amount of hemoglobin, of which the predetermined amount is metered.08-04-2011
20110189714MICROFLUIDIC CELL SORTER AND METHOD - A biological particle sorting device and method of its use. The device includes structure arranged to urge biological particles into substantially single file travel through an interrogation zone. Operable alignment structure nonexclusively include sheathed fluid flow, capillary tubes, an orifice, and fluid microchannels. One or more detector, selected from a plurality of operable such structures, may be employed to sense the presence of a biologic particle in the interrogation zone. Certain exemplary detectors may operate on the Coulter principle, or may detect a Stokes' shift, or side-scatter radiation. Discrimination structure is generally provided to categorize particles as being in one or another sub-population of a mix of biological particles that may be carried in a fluid sample, such as by cell type, size, or the like. Killing structure, such as a laser, is disposed to neutralize all particles in any undesired sub-population while leaving undamaged the desired sub-population(s). The device may be operated to essentially purify (in a living or viable sense) a sample including biological particles that are carried in a fluid diluent.08-04-2011
20110262953Phosphate Limited Inducible Promoter and A Bacillus Expression System - An evolvable production strain of 10-27-2011
20110262955METHODS FOR DETERMINING SODIUM-PROTON-EXCHANGER LIGAND EFFICIENCY - The present invention relates to methods for determining the efficiency of an ion channel ligand comprising (a) contacting a cell expressing the ion channel with (i) plasma of an animal and (ii) the ion channel ligand and (b) determining the effect of the ion channel ligand on the cell.10-27-2011
20110262954SHUTTLE VECTOR BASED TRANSFORMATION SYSTEM FOR PYROCOCCUS FURIOSUS - The present invention relates to vectors for transforming archaea and to transformed archaea, and in particular to shuttle vector systems for transformation of members of the genus 10-27-2011
20110189716METHOD FOR ANALYZING COLLAGENOUS TISSUES FOR THE DETECTION AND DIAGNOSIS OF BONE DISEASE - Methods and systems for diagnosing a bone disease related to collagen pathology in a subject are provided. These include providing a bone sample from the subject and determining a quantitative collagen morphology value of the bone sample. A reference value is provided from a non-affected control subject where the reference value is a quantitative collagen morphology value from the same type of bone sample obtained from a population of non-affected control subjects. The quantitative collagen morphology value of the subject's bone sample is compared to the reference value. If the collagen morphology value is altered versus the reference value, the subject is diagnosed as having a collagen related bone disease. The collagen morphology value can include mean fibril spacings and distributions of the fibril spacings taken from a subject's bone sample.08-04-2011
20110189713Device for the Preparation and Fractioned Dispensing of Fluid Samples, Dispensing System Including Such Device and Related Method - The present invention relates to a device, to a system, and to a method for the preparation and fractioned dispensing of samples of a fluid. The device of the invention comprises a body having formed therein guide means suitable for receiving a sample-taker member and for guiding it in translation through the device, and at least one preparation chamber enabling an aliquot of a fluid sample dispensed into the chamber by a said sample-taker member to be prepared in a stream of a suitable reagent. The guide means pass through the preparation chamber and communicate therewith to enable an aliquot of fluid to be dispensed into the chamber in a determined position of the sample-taker member in the guide means. The preparation chamber has an introduction orifice for introducing at least one reagent into the chamber for mixing the reagent with an aliquot, and at least one dispensing orifice for dispensing the mixture formed by said aliquot and said reagent to recovery and/or analysis means.08-04-2011
20100028932GENERAL PROGNOSTIC PARAMETERS FOR TUMOUR PATIENTS - Described is a method for identifying the prognosis for improved clinical benefit of an individual suffering from a tumor comprising providing a blood sample of said individual, (a1) determining the number of lymphocytes in the blood of said individual, and/or (a2) determining the number of neutrophils in the blood of said individual, and (b1) identifying the individual as having a good prognosis for improved clinical benefit, if the number of lymphocytes is above a lymphocyte baseline level of 1.4 to 1.8×1002-04-2010
20100028937TEST STRIP FOR DETECTING GASTRIC PROBLEMS AND DETECTING METHOD THEREOF - The present invention provides a test strip and a detecting method thereof. The test strip comprises a first sheet, a second sheet a first filter and a second filter. The first sheet comprises a bulge portion deposited substantially in the center thereof. The second sheet comprises a depression in alignment with the bulge portion to form a sealing space. The first filter is disk-shaped and deposited in the sealing space. The first filter is used to receive a biological sample. The second filter is ring shaped and disposed in the sealing space and under the first filter to receive the biological sample coming from the first filter. The first filter comprises sodium azide, sodium dihydrogen phosphate, and urea, and the second filter comprises sodium azide, phenol red, and urea.02-04-2010
20100028936DETECTION OF CANCER CELLS IN VITRO USING SIGMA-2 RECEPTOR LIGANDS AS RADIOTRACERS - Methods for detection of cancer cells and for determining the proliferative status of cells in a tissue sample in vitro are disclosed. These methods comprise contacting a tissue sample with a radiolabeled compound or salt thereof of Formula02-04-2010
20100028929NUCLEIC ACID MOLECULES ENCODING GPR84 - The present invention is an isolated nucleic acid molecule encoding CD36, G02-04-2010
20100028931Neurodegenerative diseases and methods of modeling - Disclosed are embryonic stem cells and motor neurons derived from mice carrying transgenic alleles of the normal or mutant human SOD1 gene. Also disclosed are in vitro systems employing such SOD1 transgenic motor neurons for the study of neural degenerative disease.02-04-2010
20100028930HEMATOPOIETIC CELL PHENOTYPING USING CIRCULATING CELL-FREE MARKERS - The present invention provides methods of classifying cluster of differentiation (CD) marker phenotype for hematopoietic cancer cells using multiple circulating cell-free CD markers in bodily fluid. In other aspects, treatment and disease progression of particular hematopoietic cancers can be monitored by measuring the levels of CD and other markers i bodily fluids of a patient.02-04-2010
20100021956Fatty Acid Pattern Analysis for Predicting Acute Coronary Syndrome - The present invention provides blood based methods for predicting risk of acute coronary syndrome in a subject.01-28-2010
20100021955MODELLING IN YEAST OF THE MITOCHONDRIAL ATP6 GENE MUTATIONS RESPONSIBLE FOR NARP SYNDROME IN HUMANS AND USES THEREOF FOR SCREENING FOR MEDICAMENTS - Modified yeast cells comprising at least one mutation of the tryptophan 136 (W01-28-2010
20100021958FLUORESCENT pH DETECTOR SYSTEM AND RELATED METHODS - Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.01-28-2010
20100021954High capacity nanoparticulate immobilization surface - Disclosed are modified substrates having polyglutaraldehyde nanoparticulates for use, for example, in binding biomolecules, and methods of making and using the modified substrates.01-28-2010
20100021953 Method for Identifying an Agent that Modulates Arginine Transport in a Chondrocyte - The present invention relates to an assay method for identifying an agent that modulates arginine transport in a chondrocyte comprising the steps of: (a) identifying an agent that modulates the activity and/or expression of CAT-2; and (b) measuring arginine transport in the chondrocyte in the presence or absence of said agent, wherein a difference between: (a) arginine transport in the absence of the agent; and (b) arginine transport in the presence of the agent is indicative that the agent can modulate arginine transport in a chondrocyte. Therapeutic agents that modulate the expression or activity of CAT-2B could be beneficial for the treatment of inflammatory diseases, particularly osteoarthritis. For example, a CAT-2B antagonist may be useful for the treatment of osteoarthritis.01-28-2010
20090317856Multimodal Spectroscopic Systems and Methods for Classifying Biological Tissue - Multimodal optical spectroscopy systems and methods produce a spectroscopic event to obtain spectroscopic response data from biological tissue and compare the response data with preset criteria configured to correlate the measured response data and the most probable attributes of the tissue, thus facilitating classification of the tissue based on those attributes for subsequent biopsy or remedial measures as necessary.12-24-2009
20100248286BIOSENSORS BASED ON MICROALGAE FOR THE DETECTION OF ENVIRONMENTAL POLLUTANTS - The present invention provides biosensors based on microalgae for the determination of the presence of toxic compounds in a sample, characterized by the high sensitivity and specificity thereof with respect to the target toxic substance. The biosensors are based on the measurement of the photosynthetic activity of algae by means of monitoring the molecular oxygen production by the microalgae by using a luminescent compound the emission of which depends on the amount of oxygen in the medium.09-30-2010
20090123960Method for Removing Antibiotics From Blood Culture Samples - Methods for removing inhibitors of microbial growth, including antibiotics, from a biological sample suspected of containing one or more microorganisms are provided. The methods include contacting a sample, or a culture growth medium containing the sample, with reversed-phase adsorbent media, which remove the inhibitors of microbial growth, but allow the microorganisms of interest to remain in the sample or culture growth medium.05-14-2009
20090148886METHODS OF IDENTIFYING COMPOUNDS THAT DECREASE INTRAOCULAR PRESSURE - This disclosure concerns methods for identifying Best2 modulators, for example methods of screening for Best2 inhibitors. In some examples, the methods include identifying compounds that alter intracellular calcium concentration, intracellular pH, or transepithelial potential in cells expressing Best2. In certain embodiments, the disclosure concerns methods of identifying compounds that decrease intraocular pressure.06-11-2009
20080254498FLUORESCENT ION INDICATORS AND THEIR APPLICATIONS - Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.10-16-2008
20090047703ERG-1 Peptides and Polynucleotides and Their Use in the Treatment and Diagnosis of Disease - The present invention relates to peptide and polynucleotide fragments of ERG-1, and in particular, human ERG-1a (HERG-1a) and its isoforms, and their use in the treatment and diagnosis of disease, especially cardiac diseases, such as arrhythmias, and cancer.02-19-2009
20120040393Directed Differentiation of Primate Pluripotent Stem Cells Into Functional Basal Forebrain Cholinergic Neurons (BFCNs) and Medium Spiny Gabaergic Projection Neurons - Methods of efficiently converting primate pluripotent stem cells to GABA neurons or cholinergic neurons, as well as applications thereof, are disclosed.02-16-2012
20120040392DOSIMETRY USING SIGMA SINGLET OXYGEN SPECTROSCOPY - A method includes measuring a photoluminescence of sigma singlet state oxygen decaying to triplet state oxygen. A dosage of delta singlet state oxygen is determined based on the photoluminescence.02-16-2012
20120040390Method for Introducing Protein and/or Peptide Into Cell - The present invention provides a method of intracellular introduction by which a protein and/or peptide can be efficiently introduced into living cells and which makes it possible to effectively analyze the behavior and function of the protein and/or peptide introduced and can be suitably utilized/applied, for example in the technology “live cell imaging” which realizes the immunohistochemical visualization in living cells under physiological conditions.02-16-2012
20100184121Removal of by-products from crosslinkable preparations - The present invention relates to the use of enzymes and/or whole-cell catalysts for the removal of undesired by-products from crosslinkable preparations.07-22-2010
20100081159Profiling reactive oxygen, nitrogen and halogen species - This invention provides methods, kits and systems which permit simultaneous profiling of multiple reactive oxygen species (ROS), reactive nitrogen species (RNS) and/or reactive halogen species (RHS) including reactive chlorine species (RCS) and/or reactive bromine species (RBS) through multiplexed fluorescence detection of three or more indicator probes in live cells or subcellular organelles.04-01-2010
20090035807ANALYSIS OF POLYPEPTIDE PRODUCTION - The present invention is directed to methods of evaluating a process to produce a polypeptide preparation or evaluating an element of a process to produce a polypeptide preparation. The methods comprise providing a sample comprising the polypeptide, wherein the sample has not been subjected to affinity chromatography, and subjecting the sample to liquid chromatography. The sample may be a process bioreactor sample. The methods of the invention may be used to determine a value of a characteristic of the sample. The methods of the invention provide a real time or near real-time information regarding the process to produce a polypeptide.02-05-2009
20090017487BIOGAS PRODUCTION FROM BMR PLANTS - Methods and compositions to produce biogas and fertilizer from plant material that have reduced lignin content relative to a wild-type plant material, for example, a brown midrib (BMR) plant, are disclosed. Methane yields from BMR plants including BMR corn are significantly higher, thereby increasing the efficiency of methane production. Anaerobic digestion of plant material derived from BMR plants results in an increased methane yield.01-15-2009
20080213818CELLULAR IMMUNITY TEST WITH PEPTIDES ATTACHED TO A SOLID SUPPORT - The invention concerns a method for detecting cellular immunity (with respect to an antigen), using a solid support whereon is fixed an assortment of peptides constituting T cell epitopes of the antigen to be tested, kits for implementing said method.09-04-2008
20100267079Identification and isolation of transitional cell carcinoma stem cells - Transitional cell carcinoma stem cells (TCCSC) are identified. The cells can be prospectively isolated or identified from primary tumor samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.10-21-2010
20080248515Optimizing culture medium for CD34<+> hematopoietic cell expansion - The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the deepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.10-09-2008
20100178665Monitoring and Manipulating Cellular Transmembrane Potentials using Nanostructures - The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed. Nanoparticles having phospholipid coatings were found to display improved responses relative to nanoparticles having other coatings that do not promote localization or attraction to membranes.07-15-2010
20100255528Methods of monitoring angiogenesis and metastasis in three dimensional co-cultures - This disclosure relates to fluorescent cell lines and to the use of such cell lines in monitoring cellular activity, such as angiogenesis. This disclosure further relates to the use of such cell lines in a three-dimensional cell culture to monitor angiogenic and metastatic potential of tumor cells and selecting personalized therapeutics for treatment of cancer.10-07-2010
20100173346METHODS FOR DETECTING CORONARY ARTERY DISEASE - Markers are provided for detecting coronary artery disease. Levels of these markers are indicative of a patient being at risk of having or developing coronary artery disease.07-08-2010
20090239252RAPID DETECTION OF VOLATILE ORGANIC COMPOUNDS FOR IDENTIFICATION OF BACTERIA IN A SAMPLE - In various embodiments, the invention relates to a method for identifying the presence of particular bacteria in a sample. The method includes collecting a sample that includes or has been exposed to the particular bacteria and detecting, in the sample, at least one volatile organic compound indicative of the presence of the bacteria.09-24-2009
20100248291METHODS FOR IDENTIFYING, PURIFYING AND ENRICHING IMMATURE OR STEM CANCER-INITIATING CELLS FROM TUMORS AND USE THEREOF - The present invention is related to methods for the preparation of cell compositions, isolated compositions obtainable therefrom, related isolated cell compositions, kits and use thereof. More specifically, the present invention provides a method for identifying, purifying and enriching immature or stem cancer-initiating cells in a 5 sample. The cell compositions, related methods and uses according to the present invention are useful in the treatment of cancers and/or the detection of enriching immature or stem cancer-initiating cells, notably cancers of the central and peripheral nervous system, metastasis to the brain.09-30-2010
20100285517TRIMETHYLAMINE COMPOUNDS AS RISK PREDICTORS OF CARDIOVASCULAR DISEASE - Methods of characterizing a test subject's risk of having or developing cardiovascular disease are provided. The methods include using an analytic device to determine levels of choline-related trimethylamine-containing compounds such as trimethylamine N-oxide, choline, or betaine in a biological sample obtained from the subject and comparing the levels of the choline-related trimethylamine-containing compound in the subject's biological sample to a control value. The test subject's risk of having cardiovascular disease is then characterized as higher if the levels of the choline-related trimethylamine-containing compound are higher than the control value. Also provided are methods of identifying a subject at risk of experiencing a complication of atherosclerotic cardiovascular disease, and methods of evaluating the efficacy of a cardiovascular therapeutic agent in a subject with cardiovascular disease using levels of choline-related trimethylamine-containing compounds.11-11-2010
20090104643BIOLUMINESCENCE-BASED SENSOR WITH CENTRIFUGAL SEPARATION AND ENHANCED LIGHT COLLECTION - In general, embodiments of the present invention relate to a bioluminescence-based point of care device that is made up of at least one reaction well (04-23-2009
20090068699USE OF GLUTAMINYL CYCLASE INHIBITORS - An inhibitor of a glutaminyl peptide cyclotransferase, and use thereof for the treatment and/or prevention of a disease or disorder selected from the group consisting of inflammatory diseases selected from 03-12-2009
20110306081Microfluidic Device - The invention relates to a microfluidic device including a chamber having a fluid inlet, a fluid outlet and a sealable port. In some embodiments, the fluid inlet and the fluid outlet may be positioned to direct fluid flowing from the fluid inlet to the fluid outlet through the chamber. Various embodiments may include a sealable port which may be aligned with the chamber to allow material to be placed directly into, or removed from, the chamber from the exterior of the device when the sealable port is open, and to inhibit and/or prevent fluid escaping through the sealable port when the port is sealed.12-15-2011
20110306082"BENZOXAZOLE-BASED FLUORESCENT METAL ION INDICATORS" - Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.12-15-2011
20110306084Tritiated Planar Carbon Forms - Tritiated planar carbon forms and their production are provided. Methods are provided for the stoichiometrically controlled labeling of planar carbon forms capitalizing on normal flaws of carboxylic acids ubiquitously present in commercial preparations of these planar carbon forms. Alternative methods include generation of a metallated intermediate whereby a metal is substituted for hydrogen on the carbon backbone of a planar carbon form. The metalized intermediate is then reacted with a tritium donor to covalently label the planar carbon form. The tritiated planar carbon forms produced are useful, for example, for determination of a biological property or environmental fate of planar carbon forms.12-15-2011
20110306083SALT SELECTION OF MICROBIAL MUTANTS TO INCREASE BIOPRODUCT TOLERANCE, TITER, OR OSMOTIC SHOCK TOLERANCE - Methods are provided for selecting microbial strains with improved properties for fermentation and/or bioproduct production. A salt selection is employed to identify mutants with improved bioproduct tolerance, titer, or osmotic tolerance relative to a microbial strain from which they were derived.12-15-2011
20110306080DETERMINATION OF LAMOTRIGINE BY MASS SPECTROMETRY - The present invention relates to compositions and methods for analyzing analytes of interest in liquid samples by mass spectrometry, and preferably in patient samples. Preferred analytes of interest include sirolimus (rapamycin), corticosteroids, bile acids and lamotrigine (lamictal). In one embodiment, by careful selection of target ions, a number of corticosteroids can be analyzed simultaneously and without interference from closely related molecules. In another embodiment, the present methods combine high turbulence liquid chromatography with mass spectrometry performed in positive and negative mode in a single assay to enable the detection and quantification of the composition of bile acid pools. By combining mass spectrometry and high-throughput chromatography, the methods and compositions described herein can provide a rapid, sensitive, and accurate assay for use in large clinical laboratories.12-15-2011
20110306078Method for Quantitative Transfer of Analytes - A method for transferring a quantity of analytes comprising at least stages of: predisposing a substantially homogeneous mixture of a predetermined initial quantity of at least an analyte and a liquid, obtaining a known concentration value or known amount of the analyte in the mixture; introducing into the mixture at least a collecting portion (12-15-2011
20110306077Methods for the detection of alternative cellular energy (ACE) pigments and for monitoring of the ACE pathway in the diagnosis and therapy of diseases - Ultraviolet light reactive alternative cellular energy pigments (ACE-pigments) are produced in response to certain chronic viral infections, metabolic disorders and degenerative illnesses. The pigments will display differing responsiveness to direct ultraviolet (UV) light illumination in the presence and in the absence of certain triggering dyes, including neutral red. The type of response appears to reflect varying energy levels of the ACE pigments from being essentially uncharged (fluorescent but only if triggering dye is added); to partially charged (fluorescent even in the absence of a triggering dye) to more fully charged (non-fluorescent either in the presence or absence of the triggering dye). Methods are described for assessing the status of ACE pigments within localized skin and deeper lesions, and within the overall body of a human or animal subject. The methods can be used to help monitor the efficacy of therapeutic measures aimed at enhancing the ACE pathway in those in whom a deficiency in the pathway can be shown to exist.12-15-2011
20100291612Peptide Markers for Diagnosis of Preeclampsia - The present invention relates to a method for detecting preeclampsia, comprising determining the expression level of calcyclin in chorionic villi. The invention further relates to a marker for detecting preeclampsia wherein said marker is calcyclin.11-18-2010
20090148881GEOBACILLUS THERMODENITRIFICANS AS WELL AS THE SCREENING METHOD AND THE USES THEREOF - The invention provides a strain of 06-11-2009
20090117606Measuring Nanoparticle Concentrations in Tissue Using Diffuse Optical Spectroscopy - A non-invasive method to measure metal nanoparticle concentrations in bulk tissue is provided. A simple diagnostic assay to detect nanoparticle concentration in bulk tissue has been developed herein. One such provided method comprises: applying diffuse optical spectroscopy to a tissue having nanoparticles disposed therein; and applying an inverse algorithm to the light reflected from the nanoparticles. Another such method comprises: exposing tissue that comprises nanoparticles to a light source; collecting light from the tissue using an optical fiber probe; and measuring the concentration of nanoparticles in the tissue. A system is provided comprising: a tissue comprising nanoparticles; a light source arranged to illuminate a portion of the tissue; an optical fiber probe to collect light reflected from the tissue; and a spectrometer to measure the light reflected from the nanoparticles and operably connected to a computer having one or more processors and a memory.05-07-2009
20110306079WATER-DISPERSABLE NANOPARTICLES - Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.12-15-2011
20090035801Twelve (12) protein biomarkers for diagnosis and early detection of breast cancer - The invention relates to 12 identified protein biomarkers for diagnosis, determination of disease severity, and therapeutic response monitoring of patients with breast cancer. The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of up to 12 protein biomarkers, and statistical analysis of the concentration of the protein biomarkers.02-05-2009
20090325212DATA STANDARD FOR BIOMATERIALS - Provided are systems and/or methods that facilitate sensing, detecting, logging, or treatment of a condition or need of a living body using a genetically engineered organism and a data standard.12-31-2009
20110065142SEBOCYTES, SEBOCYTE-CELL LINE AND USES THEREOF - Adipose cells (sebocytes) are described, The invention especially relates to sebaceous gland cells and to a sebaceous gland cell line with the property of being continuously grown over many sub-cultures. The sebocytes are excellently suited for useful applications.03-17-2011
20100129855BLOOD CELL ANALYZER, BLOOD CELL ANALYZING METHOD, AND COMPUTER PROGRAM PRODUCT - A blood cell analyzer comprising: a detector for detecting predetermined component of leukocytes contained in a specimen; and a controller, including a memory under the control of a processor, the memory storing: correlation information relating to the correlation between leukocyte distribution data and either stab neutrophil or segmented leukocyte classification data; and instructions enabling the processor to carry out operations, comprising: (a) obtaining leukocyte distribution data of a subject based on the detection results of the detector; (b) obtaining the stab neutrophil or segmented leukocyte classification data based on the correlation information and the leukocyte distribution data of the subject; and (c) outputting the obtained stab neutrophil or segmented leukocyte classification data. A blood cell analyzing method and a computer program product is also disclosed.05-27-2010
20090098596PHOTOSTABLE FRET-COMPETENT BIARSENICAL-TETRACYSTEINE PROBES BASED ON FLUORINATED FLUORESCEINS - A fluorogenic dye having the formula04-16-2009
20100323388BIOCHIP FOR HIGH-THROUGHPUT SCREENING OF CIRCULATING TUMOR CELLS - Embodiments in accordance with the present invention relate to the use of effusive filtration to segregate tumor cells from a sample of bodily fluid. In one embodiment, fluid containing a cell is flowed down a channel having a filtration medium present along at least one side wall. The tumor cell is captured when the fluid passes through the filtration medium. Accumulated pressure on the captured tumor cell is reduced by allowing the fluid that has passed through the filtration medium to re-enter the channel. In a particular embodiment, the filtration medium may comprise side wall apertures having a width smaller than that of the cell, with downstream apertures allowing re-entry of the fluid into the channel.12-23-2010
20100240090METHODS AND PLATFORMS FOR DRUG DISCOVERY - The present invention involves methods for identifying an agent that corrects a phenotype associated with a health condition or a predisposition for a health condition. The invention also involves methods for identifying a diagnostic cellular phenotype, determining the risk of a health condition in a subject, methods for reducing the risk of drug toxicity in a human subject, and methods for identifying a candidate gene that contributes to a human disease. The invention also discloses human induced pluripotent stem cell lines.09-23-2010
20100203578APPARATUS AND METHODS FOR SEPARATING AND ANALYZING COMPONENTS IN FLUIDS - Provided are methods and devices for separating particulate analytes or aggregates of analytes from a fluid, after the separation medium of the device is saturated with the fluid. The endpoint indicating completion of the separation is determined by saturation; therefore, no precise metering of the fluid sample is necessary. The separated analyte of interest can be detected, quantitated or its migration measured in the separation medium. The measured property of the analyte can then be correlated with a parameter of interest. In some embodiments, the device can be marked to directly read the value of the parameter of interest. In one embodiment, the fluid is blood and the device includes a volumetric capillary reservoir for collecting the blood, a separation paper or indicator strip, and graduations for correlating the migration of red blood cells with hematocrit or hemoglobin concentration. The interface of red blood cells and plasma creates a readable marking that corresponds to percent hematocrit and can be read from the graduations.08-12-2010
20110111450Diagnostic Device and Method - A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force.05-12-2011
20090023176Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumetric myograph - The present invention discloses devices, systems and methods for the isovolumic measurement of vasoactivity in a blood vessel (01-22-2009
20090142790Label Free Biosensors and Cells - Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed.06-04-2009
20120040391Sepsis diagnostic test - The invention relates to a method for the extracorporeal qualitative or semi-quantitative determination of the amount of indicators for the systemic inflammatory response system (SIRS) or sepsis in the blood, blood serum, blood plasma, other body fluids or lavages or their constituents of human or animal subjects. In order to provide a test which is better than that of the prior art, with which the presence and/or the severity of SIRS or sepsis can be rapidly, economically, reliably and reproducibly determined in a sample, such as blood serum of a patient. To this end, the inventive method has the following steps in which: a cell line is prepared in a culture; in at least one first preparation, cells of the cell line are brought into contact with blood, blood serum or blood plasma, other body fluids or lavages or their constituents of a human or animal subject to be examined, and into contact with a reagent that reacts to reactive oxygen intermediates (ROI), and enters into a color, light or other measurable reaction; in at least one other preparation, cells of the cell line are brought into contact with blood, blood serum or blood plasma, other body fluids or lavages or their constituents of a human or animal control subject that is not ill with SIRS or sepsis, and into contact with a reagent that reacts to reactive oxygen intermediates (ROI), and enters into a color, light or other measurable reaction; the color, light or other measurable reactions are measured in the preparations, and; the measured values of the subject to be examined are compared with those of the control subject.02-16-2012
20120045789Method for Screening of Antiviral Agents - A method for screening for an antiviral agent capable of blocking a viral viroporin by determining whether a test agent can rescue expression of a fragment of a viral viroporin in a Single Protein Production system of 02-23-2012
20120301915SYSTEM AND AUTOMATED DEVICE FOR ANALYSING A CELL SUSPENSION - A system for analyzing a cell suspension includes at least one well for decanting (11-29-2012
20120301914High Resolution Label-Free Sensor - An optical sensor for label-independent detection, having improved spatial resolution and reduced angular sensitivity, the sensor including: 11-29-2012
20120301913Microplate Assemblies for Flux Determination by Gradient Monitoring and Methods for Using Same - Microplate assemblies and methods for using same are provided. In accordance with some embodiments, microplate assemblies are provided, the assemblies comprising a microplate well portion that forms a microplate well and that includes at least one sensor in the microplate well that responds to analyte in the well. In accordance with some embodiments, methods for determining a cell consumption rate of an analyte are provided, the methods comprising: positioning a cell in a microplate well having at least one sensor located within the well; providing analyte to the microplate well at a controlled rate; detecting a response of the sensor to the analyte in the microplate well; and determining a cell consumption rate of the analyte based on the response of the sensor to the analyte.11-29-2012
20120301912METHODS, COMPOSITIONS AND KITS FOR ASSAYING MITOCHONDRIAL FUNCTION - The invention provides methods, compositions, devices, and kits relating to the use of cholesterol-dependent cytolysins (e.g., PFOs) for measuring intracellular mitochondrial activity.11-29-2012
20120064564MICROPLATE-READER WITH A CONTROLLED GAS ATMOSPHERE, CORRESPONDING METHOD AND USE OF SAME - A microplate reader and method has at least one measuring device and a holding device for accommodating at least one microplate and for positioning samples-containing wells of microplates in relation to the measuring device. The at least one measuring device is used for detecting light emitted by samples in wells of a microplate and/or which is influenced by samples transilluminated by light in the wells. The microplate reader has a control unit for controlling the composition of a gas atmosphere surrounding the wells containing the samples. A respective use is characterized particularly in that living cells are measured in a controlled gas atmosphere, wherein the living cells are chosen from microaerophilic, optionally anaerobic and obligatorily anaerobic microorganisms as well as fungi and eukaryotic cells.03-15-2012
20110033883IMAGING DYES AND USE THEREOF - Use of a metal complex having the formula: [M(L02-10-2011
20120064563CELL-BASED SENSING SYSTEMS AND METHODS - The present disclosure describes cell-based sensors. Cell-based sensors can comprise cells coupled with a sensor for sensing change of configuration and/or movement of the cells. Such changes of configuration and/or movement of the cells can be sensed through changes to one or more parameters such as electrical, mechanical and/or optical parameters. By way of example, the sensors can be magnetic based sensors or electrochemical sensors.03-15-2012
20120064560FOLDING OF RECOMBINANT PROTEINS VIA CO-EXPRESSION OF ARCHAEAL CHAPERONES - The present invention relates to recombinant protein production, and more specifically, to methods for recovery of properly folder bioactive proteins by expressing chaperone genes from extremophilic Archaea, during recombinant protein synthesis in a host cell thereby significantly improving recovery of properly folded bioactive proteins.03-15-2012
20100003711Methods to identify therapeutic agents - As illustrated herein, cholesterol is oxidized when it is present in atherosclerotic plaques. This reaction generates cholesterol oxidation or ozonation products that can act as chemotactic attractants of macrophages, can promote differentiation of monocytes into macrophages and can increase expression of E-selectin and Class A scavenger receptor (SR-A). The present application is directed to methods of using such cholesterol ozonoation products to identify agents that can be used to treat atherosclerosis and other inflammatory artery diseases.01-07-2010
20120156713METHOD FOR ANALYZING AND/OR TREATING A FERTILIZED EGG, AND CORRESPONDING SYSTEM - The present invention relates to a method for analyzing and/or treating a fertilized egg and a system able to implement said method. According to the invention, the method comprises a step of puncturing and/or injecting substance into the albumin sac of the egg, said egg having between 5 and 19 days of incubation and said step of puncturing and/or injecting being achieved by piercing a hole in the shell next to the pointed end of the egg and passing a hollow needle in said hole such as to lead the distal end of said needle into the albumin sac.06-21-2012
20090239254Functional pigmented skin equivalent - An in vitro skin equivalent includes at least one epidermis equivalent and at least one dermis equivalent, and further includes melanocytes constitutively producing melanin and fibroblasts.09-24-2009
20120309043EMBRYO QUALITY ASSESSMENT BASED ON BLASTOMERE DIVISION AND MOVEMENT - The invention concerns a system and method for determining embryo quality comprising monitoring the embryo for a time period, said time period having a length sufficient to comprise at least one cell division period and at least a part of an inter-division period, and determining the length of the at least one cell division period; and/or ii) determining the extent and/or spatial distribution of cellular or organelle movement during the cell division period; and/or iii) determining duration of an inter-division period; and/or iv) determining the extent and/or spatial distribution of cellular or organelle movement during the inter-division period thereby obtaining an embryo quality measure. Thus, the selection of optimal embryos to be implanted after in vitro fertilization (IVF) is facilitated based on the timing, duration, spatial distribution, and extent of observed cell divisions and associated cellular and organelle movement.12-06-2012
20110318774DUAL SAMPLE CARTRIDGE AND METHOD FOR CHARACTERIZING PARTICLES IN LIQUID - The present invention relates to an apparatus for characterizing particles suspended in a liquid, especially a self-contained disposable cartridge for single-use analysis, such as for single-use analysis of a small quantity of whole blood. Furthermore, the present invention relates to a method for characterizing particles in liquid and a device for sampling a small and accurate volume of liquid. The apparatus comprises a housing having a mixing chamber and a collection chamber separated by a wall containing an opening, a first bore in the outer surface of the housing for entrance of a liquid sample, a first cavity for receiving and holding a first liquid sample, and a second cavity for receiving and holding a second liquid sample.12-29-2011
20110318773METHOD TO USE EKTACYTOMETRY TO IMPROVE STORED RED BLOOD CELL OR WHOLE BLOOD PRODUCT UTILIZATION - A method for assessing the quality degradation of an RBC or whole blood unit, the method comprising: 1) using an ektacytometer to generate erythrocyte deformability data for the RBC or whole blood unit; 2) analyzing the erythrocyte deformability data to assess the quality degradation of the RBC or whole blood unit; 3) providing the erythrocyte deformability data to medical personnel; 4) correlating the erythrocyte deformability data with a clinical outcome of a patient that has used the RBC or whole blood unit; and 5) incorporating the erythrocyte deformability data into subsequent assessments of quality degradation of RBC and whole blood units. This method would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying the approach throughout the supply chain will improve distribution, planning, and inventory control decisions.12-29-2011
20110318772REACTIVE COUMARIN DERIVATIVES AND THEIR USE IN CELLULAR ANALYSES - Chemically reactive 7-hydroxycoumarin derivatives and their application for analyzing cell function, for example in combination with additional fluorescent labels. The coumarin derivatives exhibit a strong absorption at 405 nm and high fluorescence quantum yields.12-29-2011
20090170149PHOTO-ACOUSTIC DETECTION DEVICE AND METHOD - An example system for detecting an analyte in a sample of a bodily fluid comprises a test chamber having at least one sidewall and configured to contain at least a portion of a bodily fluid sample, an excitation electromagnetic energy source configured to direct an energy source into the test chamber through the at least one sidewall and to induce a thermoelastic expansion in the one or more analytes, and a sensor configured to detect said thermoelastic expansion in the bodily fluid sample in the test chamber, the sensor configured to measure changes in optical reflectance that result from the thermoelastic expansion.07-02-2009
20100136600URINE BASED DETECTION OF A DISEASE STATE CAUSED BY A PNEUMOCOCCAL INFECTION - There is provided a method of diagnosing a disease state in a subject. The disease state is caused or effected by pneumococcal infection. The method includes obtaining a biological test sample from the subject. The biological sample includes at least one metabolite selected from the group consisting of citrate, trigonelline, acetylcarnitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine. A respective concentration of each one of the at least one metabolite is compared with a predetermined concentration value associated with a corresponding one of the at least one metabolite. The predetermined concentration value is indicative of the disease state. For example, the biological sample includes any one of citrate, trigonelline, acetylcamitine, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine. As a further example, the biological samples include any combination of citrate, trigonelline, acetylcamitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine. As a further example, the biological sample includes each one of citrate, trigonelline, acetylcamitine, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine.06-03-2010
20120045786OPTO-FLUIDIC MICROSCOPE DIAGNOSTIC SYSTEM - An image sensor integrated circuit may contain image sensor pixels. Channels containing a fluid with cells or other material may be formed on top of the image sensor. The image sensor pixels may form imagers. Each imager may be located in a respective one of the channels. Reactant chambers may be used to expose the particles in the fluid to reactant. The imagers may gather images of the cells or other particles as the fluid passes over the imagers following exposure to the reactant. Spent sample chambers at the ends of the channels may be used to collect the fluid after the fluid has passed over the imagers. Image data from the imagers may be processed by control circuitry on the image sensor integrated circuit and external equipment.02-23-2012
20120045791METHOD AND A BLOOD OXYGEN TESTER FOR DETECTING THE ARTERIAL OR VENOUS BLOOD - A method and a blood oxygen tester for determining whether a blood sample is arterial or venous blood are disclosed. A blood oxygen tester for determining whether a blood sample is arterial or venous blood includes a housing and a blood sample receptacle defined by the housing. A blood oxygen sensor is in communication with the blood sample receptacle and a test result indicator is in communication with the blood oxygen sensor. The indicator is responsive to the blood oxygen sensor for indicating whether a tested blood sample is arterial blood or venous blood.02-23-2012
20120045787FLEXIBLE MICRO-CARRIER SYSTEM - Micro-carrier systems may be used to carry and identify sample materials through an analysis system. Analysis systems may include an image sensor integrated circuit containing image sensor pixels. A channel containing a fluid with particles such as cells may be formed on top of the image sensor. Micro-carriers may be used to carry the cells in the fluid. Micro-carriers may have identifier regions and active regions. Identifier regions may include coded information identifying cells, fluid samples, or other materials carried in the active region. Active regions may carry reagents, trapping agents, cells or other sample materials. Active regions may be formed on a surface of a micro-carrier or may be formed in a cavity inside the micro-carrier. Micro-carriers may include magnetic control structures that can be used to guide, rotate, accelerate or position micro-carriers.02-23-2012
20100304421OPTICAL MONITORING METHOD - A mixture of dyes each having an optical property (e.g. fluorescence intensity) which is sensitive to an external factor is caused to interact with a system that is to be monitored, such that the system provides the external factor and therefore influences the optical properties of the dyes. The optical properties of the individual dyes are individually measurable (e.g. being spectral intensities at different wavelengths). Their values are subjected to pattern analysis, e.g. using an artificial neural network analysis, leading to an output that characterises the system or its state.12-02-2010
20120171712 Process for Realising a Device for Collecting and Transferring Samples for Molecular Biology - A process for realising a device (07-05-2012
20090286278MULTI-LAYER CELL ENCAPSULATION FOR TISSUE ENGINEERING - A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.11-19-2009
20120003684Method and Means for Detecting the Activity of Osteoclasts - The invention relates to a method and means for detecting the resorption activity of osteoclasts, in particular for use in medicine and in bioscience and pharmaceutical research. Previous methods for measuring the resorption activity of osteoclasts in vitro are difficult to quantify, are partially inflexible when used with different donor organisms and require special measuring devices for data acquisition. The method and kit according to the invention advantageously use a biomineralized matrix which contains calcium phosphate and was obtained in vitro by depositing calcium phosphate by means of osteoblasts. Osteoclasts are incubated on this matrix and the non-resorbed calcium phosphate is then quantified. The method according to the invention advantageously functions with osteoclasts of different organisms and cell types and can be easily quantified.01-05-2012
20120003680ASSEMBLY OF ABSOLUTELY QUANTIFIED PEPTIDE AND PHOSPHOPEPTIDE SOLUTIONS VIA ELEMENT MASS SPECTROMETRY - The present invention relates generally to the fields of analytics of proteins and peptides. More particularly, the invention concerns generation of an absolutely quantified reference peptide or protein solution via element mass spectrometry. Furthermore, the invention relates to the absolute quantification of phosphopeptides and peptides by the use of an absolutely quantified peptide solution.01-05-2012
20120003683CELLS FOR INDICATING THE PRESERVATION OF BIOLOGICAL SAMPLES - Methods of characterizing the extent to which a biological sample preserved at a very low temperature has been exposed to stress using indicator cells is provided.01-05-2012
20120003682MICROFABRICATED APPARATUS FOR CELL BASED ASSAYS - Apparatus and methods are provided for performing cell growth and cell based assays in a liquid medium. The apparatus comprises a base plate supporting a plurality of micro-channel elements, each micro-channel element comprising a cell growth chamber, an inlet channel and an outlet channel, a cover plate positioned over the base plate to define the chambers and connecting channels. Means are incorporated in the cell growth chambers, for cell attachment and cell growth. In particular, the invention provides a rotatable disc microfabricated for performing cell growth and cell based assays. The apparatus and method can be used for the growth of cells and the detection and measurement of variety of biochemical processes and products using non-invasive techniques, that is techniques which do not compromise the integrity or viability of cells.01-05-2012
20120003681EX VIVO METHOD FOR DETERMINING POTENTIAL GLP-2 RECEPTOR MODULATIONS - Disclosed herein is a method for measuring the contractility of intestinal tissue upon treatment with GLP-2 or a GLP-2 ligand. Also disclosed is an assay which directly measures the activity of GLP-2 or GLP-2 ligands ex vivo and permits the screening of putative GLP-2 ligands in native tissue.01-05-2012
20120003679SENSOR MODULE, TISSUE PROCESSOR, AND METHOD FOR OPERATING A TISSUE PROCESSOR - A sensor module (01-05-2012
20120115178Method of Diagnosing Gastric Cancer by Using Human Neutrophil Peptide 1-3 - Human neutrophil peptide (HNP) 1-3, used as diagnostic and therapeutic molecular probes, are found in clinical tissues of gastric cancer patients. In the analytical process according to the present disclosure, pairs of gastric cancer tissues are used to seek the putative biomarkers by proteomic strategy based on matrix assisted laser desorption ionization-imaging mass spectrometry (MALDI-IMS). Then, three differential biomarkers, including HNP-1, -2 and -3, are identified (P<0.001) and overexpressed in gastric cancer. At last, western blotting and immunohistochemistry are used to validate the protein expression in gastric cancer tissues. In conclusion, the use of the up-regulated proteins, HNP1-3, helps diagnosis and therapy in clinical for gastric cancer after validating the sensitivity and specificity.05-10-2012
20110027817TAU PROTEIN SCREENING ASSAY - This invention is directed to methods for determining if a test compound can ameliorate tau protein induced reduction of long term potentiation in a neural structure. The invention is also directed to methods for determining if a test compound can re-establish or rescue synaptic function in a neural structure following damage by tau proteins. Also encompassed by disclosures in this invention are methods to determine if a test compound can increase synaptic function in a neural structure contacted with tau proteins and methods for determining if a test compound is capable of treating Alzheimer's disease or other tauopathies in a subject.02-03-2011
20120045788Device for sample preparation - A pipette tip or tube containing chromatographic media contained and held in place in said tube by using low melting point porous polymer particles. Such pipette tips or tubes can be used for sample preparation, filtration and synthesis of small molecules and biomolecules.02-23-2012
20110053201METHOD OF CELL SEPARATION - A method for the cell separation from blood or from blood products, having the following steps: 03-03-2011
20120009616DETECTION OF OLIGOSACCHARIDES - Provided herein are processes for detecting oligosaccharides in a biological sample. In specific instances, the biological sample is provided from an individual suffering from a disorder associated with abnormal glycosaminoglycan accumulation.01-12-2012
20120009618ENDOTHELIAL CELL PRODUCTION BY PROGRAMMING - The invention generally regards methods for providing endothelial cells and precursors of endothelial cells from a variety of cell sources, such as pluripotent stem cells. Also provided are therapeutic compositions including the provided endothelial cells, and methods of using them for the treatment of subjects.01-12-2012
20120009615DIPYRROMETHENE-BORON HYDROPHILIC FLUORESCENT COMPOUNDS - Dipyrromethene-boron hydrophilic fluorescent compounds01-12-2012
20120009617ZINC SENSORS FOR CELLULAR IMAGING - This present invention provides new compounds for use as zinc ligand sensors in cellular imaging. These compounds are highly sensitive imagining agents that can be used with techniques such a laser scanning microscopy in intact cells in culture or in tissue preparations.01-12-2012
20090221018Procine circovirus type 2 vaccines - The present invention is based on the ORF3 gene of Porcine Circovirus Type 2 (PCV2) and the identification of tis apoptotic role. This discovery has led to the development of an attenuated live vaccine against PCV2.09-03-2009
20090181418PEPTIDE ABLE TO BREAK THE M-P53/P63, M-P53/P73 AND M-P53/RESPECTIVE ISOFORM PROTEINS COMPLEX FORMED IN THE TUMOR CELLS AND USES THEREOF IN THE PHARMACOLOGICAL FIELD - The object of the present invention is the identification of a group of peptides able to break the interaction between the mutated protein p53 (hereinafter m-p53) and the proteins p63, p73 and the relative isoform proteins (hereinafter p63, p73 and p-isoforms) in the m-p53/p63, m-p53/p73 and m-p53/p-isoforms proteinic complex that has formed in the nucleus of tumor cells. Furthermore, the present invention relates to a method for causing the breakage of said proteinic complexes existing in the tumor cell lines in vitro. The present invention further relates to the use of said peptides for the preparation of a pharmaceutical composition for treating human tumors.07-16-2009
20120015396COMPOSITIONS AND METHODS FOR MODULATING THE ACID-SENSING ION CHANNEL (ASIC) - Novel compositions for modulating acid-sensing ion channels (ASIC) function comprising ASICα, ASICβ, and BNC1 and derivatives thereof; methods for modulating ASIC function and methods for treating cognitive disorders and for memory enhancement using the novel compositions of the invention; and a method for increasing synaptic plasticity are described.01-19-2012
20120015391BIOCHEMICAL REACTOR - A biochemical reactor involves an online cell examination microscope (01-19-2012
20120015394Transfer Unit and Method for Receiving a Medium From a Processing Device - The invention relates to a transfer unit for receiving in particular a porous disc-shaped medium from a first treatment device, having an upper part, wherein the upper part comprises a fixing edge that can be connected to an edge of the medium for removing the medium from the first treatment device, wherein the upper part comprises an opening closed by a removable cover, by means of which successive treatments in a further treatment device can be performed. The invention further relates to a method wherein an upper part of a transfer unit is placed on a disc-shaped medium disposed in a first lower part of a first treatment device and exposed to the liquid sample and is connected to an edge of the medium, wherein the upper part is lifted off from the first lower part with the disc-shaped medium connected thereto, and is placed on a further lower part of a corresponding treatment device, and is further processed through an opening in the upper part.01-19-2012
20120015392Cell Counting Slide With Lateral Reservoir For Promoting Uniform Cell Distribution - Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber.01-19-2012
20090162890IDENTIFYING THERAPEUTIC COMPOUNDS BASED ON THEIR PHYSICAL-CHEMICAL PROPERTIES - The present invention is directed to rapid and efficient methods of identifying therapeutic compounds by allowing only the most favorable molecules initially selected based on their physical-chemical profile falling within a range predefined by the physical-chemical/biological relationship of a previously tested small subset of compounds of same core structure to be assayed; and to the therapeutic compositions identified by said methods.06-25-2009
20090162888SAMPLE CONTROL FOR CORRECTION OF SAMPLE MATRIX EFFECTS IN ANALYTICAL DETECTION METHODS - Methods and systems are described suitable to determine the effects of sample matrix on the detection of a label so as to allow correction for these sample matrix effects when using the label in an analytical detection technique. The method is particularly advantageous for use in a disposable molecular diagnosis cartridge.06-25-2009
20110165611DUAL MODALITY DETECTION OF APOPTOSIS - To image apoptosis in vivo, small, membrane-permeable probes comprising a caspase 3 substrate, a fluorogenic dye and a radionuclide is provided. This dual-modality probe can be cleaved by caspase upon exposure to apoptotic cells, allowing imaging of caspase 3 and 7 activities using both optical and nuclear imaging techniques. The combined use of these methods provides the opportunity for a direct correlation between in vitro and in vivo biological activities and a viable method to treat disease07-07-2011
20110165612NOVEL YEAST STRAINS FOR PRODUCING ALCOHOL - The present invention relates to novel stable yeast strains, to a novel method for obtaining such strains, and to a novel method for evaluating the stability of a yeast strain. The present invention also relates to the yeasts obtained from these novel stable yeast strains, and to the use thereof for producing alcohol.07-07-2011
20110165609DEVICE, A SYSTEM AND A METHOD FOR MONITORING AND/OR CULTIVATION OF MICROSCOPIC OBJECTS - The present invention relates to a device, a system and a method for performing monitoring and/or cultivation of microscopic objects. Microscopic objects are in particular microscopic organisms like bacteria and cell cultures, such as cultivation objects like tissue samples and embryos, providing optimal and safe cultivation conditions for incubation during embryo development and for facilitating the selection of optimal embryos to be used in in vitro fertilization (IVF) by facilitating embryo handling for automated digital imaging and time-lapse microscopy.07-07-2011
20110165610COMPOSITIONS, SYSTEMS, AND METHODS FOR PRESERVATION AND/OR STABILIZATION OF A CELL AND/OR MACROMOLECULE - The present disclosure relates to compositions, systems, and methods for preserving and/or stabilizing a cell (e.g., a whole cell). A cell and/or macromolecule stabilizing composition may include a chelator, a chelator enhancing component, and optionally a base (e.g., a purine base or a pyrimidine base). A cell stabilizing method may include contacting a cell with a cell and/or macromolecule stabilizing composition. A cell stabilizing system may include a container suitable for receiving a sample containing a cell and a cell and/or macromolecule stabilizing composition. A cell may be preserved and/or stabilized under ambient conditions (e.g., without refrigeration). A cell may include a protein, a nucleic acid, and/or another biomolecule marker of cell preservation and/or stabilization. A composition may be configured to preserve and/or stabilize one or more cells for analysis by flow cytometry and simultaneously preserve and/or stabilize one or more intracellular nucleic acids for molecular analysis.07-07-2011
20090098593Laboratory plate thermal vault - A portable temperature transfer device for transferring thermal energy to and/or from a laboratory plate is provided as well as its methods of use. The temperature transfer device comprises a base and a raised stage that comprises a thermal conductive material and an encasement that interfaces with the base to create a cavity that encloses a laboratory plate. The raised stage allows direct contact between individual wells of the laboratory plate and the temperature transfer device. The encasement completes the thermally conductive environment for the laboratory plate.04-16-2009
20120058506MICRONEEDLE - The application relates to microfluidic needles and their manufacturing methods. The microfluidic needle comprises an interface portion containing at least one fluid communication channel, an elongated needle portion (03-08-2012
20090004687PREDICTIVE MARKERS FOR OVARIAN CANCER - Methods are provided for predicting the presence, subtype and stage of ovarian cancer, as well as for assessing the therapeutic efficacy of a cancer treatment and determining whether a subject potentially is developing cancer. Associated test kits, computer and analytical systems as well as software and diagnostic models are also provided.01-01-2009
20120058504METHODS AND APPARATUS FOR DIELECTROPHORETIC SHUTTLING AND MEASUREMENT OF SINGLE CELLS OR OTHER PARTICLES IN MICROFLUIDIC CHIPS - The invention relates to a microfluidic device. The microfluidic device comprises a fluid chamber comprising a particle retention region for retaining at least one particle, such as a cell. The microfluidic device also comprises a plurality of electrodes extending into the particle retention region for applying a dielectrophoretic (DEP) force to controllably move the particle within the particle retention region. The invention also relates to methods of using the microfluidic device to controllably move the particle within the microfluidic device and to monitor, observe, or measure a parameter of the particle. The particle movement may be caused by a DEP force and/or a fluidic force.03-08-2012
20120058505Cytosolic Delivery of Materials with Endosome-Disrupting Colloids - A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.03-08-2012
20100093014SCREENING FOR BONE ANABOLIC FACTORS - A method for screening for modulators of the secretion by an osteoclast of a wnt or a wnt signal enhancer comprises exposing osteoclasts in culture to a compound to be screened, exposing a wnt sensitive detection system to conditioned medium from said osteoclast culture, and determining whether a wnt signal is present in said medium by assaying for wnt mediated activation of bone formation by osteoblasts or by wnt mediated activation of LRP5 and or LPR6 signalling in a cell by detection of β catenin or detection of translocation of disheveled, axin, or Frat1 to the cell membrane of said cell.04-15-2010
20120156712SYSTEM AND METHOD FOR IDENTIFICATION OF BIOLOGICAL TISSUES - The present invention provides for a system, method, and device for analyzing, localizing and/or identifying tissue types. The method includes analyzing, localizing and/or identifying one or more tissue samples, characterized in that the method comprises: (a) generating gaseous tissue particles from a site in the one or more tissue samples, (b) transporting the gaseous tissue particles from the site to an analyser, (c) using the analyser for generating tissue-related data based on the gaseous tissue particles, and (d) analyzing, localizing and/or identifying the one or more tissue samples based on the tissue-related data. The invention can either be used in close conjunction with a surgical procedure, when one or more surgical tools are an integrated part of ionization, or as a separate mass spectrometric probe for the analysis of one or more tissue parts.06-21-2012
20120156711System and method that allows the joined performance of optoelectric and respirometric sensors for instant and accurate ascertainment of biochemical oxygen demand (BOD) in liquid industrial wastes - The present invention provides a system and method that combines the rapidity of the optoelectric signal and the simplicity of the respirometric cartridge wherein the greater accuracy of the respirometric biosensor is added to the rapidity of the optoelectric sensor, with the purpose of improving the accuracy of the measurements and the velocity of the integrated monitoring process and online control of the environmental variable of interest, removing the operative restrictions of work ranges.06-21-2012
20100028935CONVEX BOTTOM MICROWELL - Convex-bottom microwells for laboratory use in optical analyses of colorimetric or enzymological type useful for avoiding interferences with the luminous beam of the detecting instrument by corpuscular elements present in the sample to be analysed.02-04-2010
20120208229DEVICE FOR EXAMINING CELLS HAVING AN ELASTOMER, AND USE OF THE DEVICE - A device for examining cells comprising an elastomer and the use thereof. The elastomer comprises a bottom and a thicker edge region; and the bottom is provided with regular microstructures. Such elastomers are suitable for stretch experiments, notably of the uniaxial type.08-16-2012
20120070857ELECTROPHYSIOLOGICAL ASSAYS USING OOCYTES THAT EXPRESS HUMAN ENaC AND THE USE OF PHENAMIL TO IMPROVE THE EFFECT OF ENaC ENHANCERS IN ASSAYS USING MEMBRANE POTENTIAL REPORTING DYES - In one aspect, the present invention relates to a mammalian cell-based high-throughput assay for the profiling and screening of human epithelial sodium channel (hENaC) cloned from a human kidney c-DNA library and is also expressed in other tissues including human taste tissue. The present invention further relates to amphibian oocyte-based medium-throughput electrophysiological assays for identifying human ENaC modulators, preferably ENaC enhancers. Compounds that modulate ENaC function in a cell-based ENaC assay are expected to affect salty taste in humans.03-22-2012
20090317854Recombinant adenylate cyclase of BORDETELLA SP. for diagnostic and immunomonitoring uses, method of diagnosing or immunomonitoring using said recombinant adenylate cyclase, and kit for diagnosing or immunomonitoring comprising said recombinant adenylate cyclase - Diagnostic testing and immunomonitoring that uses genetically detoxified 12-24-2009
20110091929MEANS AND METHODS FOR ASSESSING LIVER ENZYME INDUCTION - The present invention pertains to the field of toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing the pro-pathological effect of compounds which are inducing liver enzymes. It also relates to a method of determining whether a compound is capable of exhibiting pro-pathological effects on the liver by enzyme induction in a subject and to a method of identifying a drug for treating the pro-pathological effect of liver enzyme induction. Furthermore, the present invention relates to a data collection comprising characteristic values of at least five analytes, a data storage medium comprising said data collection, and a system and a device for diagnosing the pro-pathological effect of liver enzyme induction. Finally, the present invention pertains to the use of a group of analytes or means for the determination thereof for the manufacture of a diagnostic device or composition for diagnosing the pro-pathological effect of liver enzyme induction in a subject. For each sex, a different metabolome pattern, i.e. a different set of analytes is disclosed. The liver enzyme induction markers are mainly selected from free fatty acids, but also include various phosphatidylcholines, galactose, 3- and 5-Methoxysphingosine, Cholesterol, Threonic acid, 1,2-Dioleoyl-sn-glycero-3-phosphatidyl-L-serine, Glycerol, Glycerophosphate, Dodecanol, myo-Inositol-2-monophosphate.04-21-2011
20110091928Agent for Recruitment of Bone-Marrow-Derived Pluripotent Stem Cell Into Peripheral Circulation - The present invention for the first time demonstrated that:04-21-2011
20110091927MOTOR NEURONS DEVELOPED FROM STEM CELLS - The present invention provides a method for directing differentiation of neural progenitors with a caudal and ventral specification into motor neurons comprising culturing neural progenitors in a culturing medium comprising a basic medium supplemented by at least one inhibitor of the Notch signaling pathway whereby said neural progenitors differentiate into postmitotic motor neurons. The resulting motor neurons may be used for drug development, as carriers, e.g. for gene therapy of protein delivery as well as for transplantation for the purpose of treating a motor neuron disease.04-21-2011
20110091925Processing of nanoparticles - Ligand-capped nanoparticles are dispersed in an organic solvent. There is then phase transfer of the nanoparticles introducing into the organic solvent an aqueous solution of polymer surfactant dissolved in water. The organic solvent and the aqueous solution are then mixed until the polymer forms micelles which encapsulate the nanoparticles in assemblies. The resultant nanoparticle assemblies in an aqueous phase may be used for any of a range of desired applications. It has been found that the assembly size can be tuned by control of any or a combination of method parameters such as concentration of polymer surfactant, and/or temperature of the phase change reaction, and/or rate of mixing, such as rotational rate of stirring. The nanoparticle assemblies find particular application as fluorescent biomarkers.04-21-2011
20110091924Method of evaluating cancer type - According to the method of evaluating cancer type of the present invention, amino acid concentration data on concentration values of amino acids in blood collected from a subject to be evaluated is measured, and the cancer type in the subject is evaluated based on the concentration value of at least one of Glu, ABA, Val, Met, Pro, Phe, Thr, Ile, Leu, and His contained in the measured amino acid concentration data of the subject.04-21-2011
20110091923Compact Optical Reader System - A method and optical reader system for label-independent detection as defined herein. The reader system includes: a launch beam; a first lens; a receptacle for receiving at least one optical biosensor article, the article having a mask on one face, and the mask having at least one aperture there through for receiving and transmitting radiation from the collimated launch beam; an angular separator; and an imager to record the image of the optical biosensor article.04-21-2011
20110091922Bio-Matrix Stretcher - Embodiments provide techniques for measuring and characterizing the dynamics of cell traction forces. Tunable elastic gel substrates can be disposed in multi-well plates. The gels can be of a uniform predetermined thickness. A multi-well plate can be loaded with gels of different shear moduli. An array of punch indenters can be attached to a loading platen such that the each indenter is aligned to a gel substrate. The indenters can apply tensile or compressive strains to the gel substrates. The magnitude, duration, and frequency of the strain can be controlled by a motor assembly coupled to a control system. The apparatus can be disposed in an incubator for long term cell culture experiments. The cell culture can be observed while a strain is applied. A ring-shaped indenter can be mounted on a microscope, coaxial to the objective lens, and lowered by a calibrated amount onto the underlying gel.04-21-2011
20120156714INTEGRATED CYTOMETRIC SENSOR SYSTEM AND METHOD - The invention provides a flow cytometric system comprising a first sensor positioned axially to a light source; a channel comprising means for receiving a sample target and interposed between said first sensor and light source; and a second sensor placed at an angle to said first sensor adapted to sense side scattering and/or fluorescent components and said first sensor is adapted to sense a forward scattering component in response to light illuminating the sample target in said channel. In another embodiment the invention provides for a wide dynamic range sensor comprising a plurality of photodiode pixels; wherein at least one or more of said photodiode pixels are voltage biased in one or more of the following modes: photon counting, normal, linear avalanche or Geiger modes, for wide dynamic sensor range operation. By altering the reverse bias voltage, thus putting each photodiode into one of normal, avalanche or Geiger mode, the dynamic range of incident scattering and fluorescent power to which the filter cell array is sensitive to is greatly increased, thus increasing the operational sensitivity and specificity of the cytometric instrument.06-21-2012
20110065144METHOD OF MEASURING EFFECTS OF COMPONENTS ON CELL REACTIVE OXYGEN SPECIES PRODUCTION - A method of determining the effects of a test component on intracellular ROS levels. The method can be used in marketing or in screening for useful components in reducing ROS levels in cells adversely affected by increased ROS generation.03-17-2011
20110065143Multiphoton Scanning Flow Cytometer for Multicellular Aggregates - A flow cytometry system suitable for characterizing multicellular aggregates during culture and before implantation combines a low shear flow channel with a multiphoton laser scanning microscope, the latter permitting the characterization of interior and exterior cells in optical isolation from other cells for a representative sampling of fluorescent activity. Imaging capabilities permit sophisticated statistical measurements reflecting individual cell characteristics.03-17-2011
20110104738BLOOD VISCOSITY ANALYSIS - A blood analyzing device (05-05-2011
20110104735HUMAN OMENTAL MESOTHELIAL CELLS, METHODS OF ISOLATION AND USES THEREOF - The present invention discloses novel methods and omental, myocardial, liver, lung, renal, peritoneal, intestinal and pancreatic mesothelial cells which are useful for a number of procedures including drug discovery, co-culturing, cell therapy and bioassay. The invention provides a method for isolating these cells that improves upon the methods previously used and provides cells isolated in quantity. The present invention provides a list of secreted proteins from omentum mesothelial cells that can be utilized in the described cell based assays.05-05-2011
20110104732SURFACE-STRUCTURED DEVICE FOR LIFE-SCIENCE APPLICATIONS - Embodiments of the invention relate a surface-structured device for life-sciences and a life-science method applying the surface-structured device. The surface-structured device has a substrate with a frontside surface corresponding to a first surface; and a plurality of protrusions arranged on the frontside surface. A shortest dimension of the protrusions at the junction from the protrusion to the front-side surface is smaller than 250 nm and at least a first group of the plurality of protrusions is arranged on a first planar area of the frontside surface in a first regular pattern in a plane of the first planar area of the frontside surface. Further embodiments relate to a stamper which may be used in the manufacturing method of the surface-structured device and a manufacturing method for surface-structured device.05-05-2011
20110104731REACTION CASSETTE, ASSAY DEVICE, AND ASSAY METHOD - A reaction cassette for biochemical assay, a biochemical assay device including the reaction cassette, and a biochemical assay method performed by using the biochemical assay device are provided. The reaction cassette includes a first space, a second space, a third space, and an inner wall. The first space is configured to accommodate liquid and includes a first opening facing upward. The second space includes a second opening whose direction is perpendicular to the direction of the first opening. The first space and the second space are disposed such that when the reaction cassette is rotated, liquid in the first space can flow into the second space. The third space is located under the first space and includes a third opening whose direction is the same as the direction of the first opening. The inner wall connects the second opening and the third opening, which serves as a liquid flow channel between the second space and the third space.05-05-2011
20110104737Photometric measuring method for a sample liquid, a photometric measuring device, and a mixing container for a photometric measuring device - The invention relates to a mixing container (05-05-2011
20110104741Screening Method for Inhibitors of Cancer Cell Invasion and Screening System Thereof - This invention relates to a screening method for an inhibitor to cancer cell invasion, comprising the steps of: (a) co-culturing cancer cells and a carcinoma-associated fibroblasts (CAFs) in a multi-chamber containing a upper-chamber, a lower-chamber and a porous filter separating the upper-chamber from the lower-chamber; in which each cancer cells and CAFs is inoculated into the upper-chamber and the lower-chamber of the multi-chamber, and then a candidate is added to the upper-chamber; and (b) measuring the number of cancer cells passing the porous filter. According to the screening system and screening method using the same, the inhibitor to cancer cell invasion is able to be screened in a high-throughput manner.05-05-2011
20110104733Method for Increasing the Biomass and the Metabolic Activity of Microorganisms by the Combined Adjustment of the Oxidation-Reduction Potential and of the Oxygen Dissolved During the Fermentation Process - The invention relates to a method for cultivating microorganisms, particularly of the type that comprises the step of seeding a culture medium with one or more microorganism strains, and the step of cultivating the medium thus seeded, characterized in that it comprises, during the entirety or a portion of the cultivation, the two following and simultaneous adjustments: adjusting the amount of oxygen dissolved in the medium to a given dissolved-oxygen setpoint; adjusting the value of the redox potential Eh of the medium to a given setpoint value Eh.05-05-2011
20110104740FLUORESCENT PROTEIN AND CHROMOPROTEIN - It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from 05-05-2011
20110104736SELECTION OF ORGANISMS CAPABLE OF FERMENTING MIXED SUBSTRATES - The present invention relates to a method for selecting a strain of an organism capable of improved consumption of a mixed substrate comprising two or more carbon sources as compared to a reference strain of the organism, which method comprises: growing a population of the reference strain of the organism in the presence of the two or more carbon sources, wherein the number of generations of growth of the said population on each of the said carbon sources is at least about 50% of the number of generations of growth on the carbon source most preferred by the organism; and selecting the resulting strain of the organism, thereby to select a strain of the organism capable of improved consumption of a mixed substrate comprising the two or more carbon sources as compared to the reference strain of the organism. The invention also relates to strains of organisms selected using such a method. Strains of organisms identified using the selection method may be used in fermentation processes in which a mixed substrate is used.05-05-2011
20110104730MESOSCALE BIOREACTOR PLATFORM FOR PERFUSION - Disclosed is a mesoscale bioreactor platform including two or more liquid reservoirs in fluid communication with a culture chamber which chamber is in fluid communication with an exit. The platform allows the chamber to be perfused with a flow of liquid from one or more of the liquid reservoirs. The integrated reservoirs for liquids remove the need for external supplies of liquid to the culture chamber during cell culture experiments requiring perfusion of liquids. Moreover, with two or more reservoir it is possible to supply the culture chamber with different types of liquids.05-05-2011
20110104729CELL CULTURE SYSTEM, CELL CULTURE METHOD, CELL CULTURE VESSEL AND METHOD FOR MANUFACTURING CELL CULTURE VESSEL - A cell culture system comprising a light source for emitting light, a light intensity regulator for regulating the light intensity of the light emitted by the light source, a cell activity-measuring device for measuring the activity of cells cultured on a photocatalytic film irradiated with the light, and an association device for associating the light intensity with the cell activity.05-05-2011
20120122138CONSUMABLE COMPONENT KIT - According to the invention there is provided a consumable component kit for use in a system which utilises a liquid culture of a microbiological material, the kit including: a sealed and aseptic culturing vessel; at least one sealed and aseptic chamber for storing a liquid nutrient medium; a sample of the microbiological material; a sample of the nutrient medium which is suitable for use in a feedstock for the microbiological material; optionally, a sample of salts and other components of the liquid nutrient medium; and a plurality of aseptic conduits for connecting the chamber to the culturing vessel, and for connecting the culturing vessel to the remainder of the system to enable a liquid supply of the microbiological material, or a product or extract thereof, to be utilised by the system.05-17-2012
20120122140ULTRASONIC METHOD FOR MONITORING CELL CULTURES - A method for monitoring a cell culture, where the method includes measuring pulse-echo ultrasonic waveforms from the cell culture, and analyzing the pulse-echo ultrasonic waveforms to monitor the cell culture.05-17-2012
20100093016MICROFLUIDIC APPARATUS AND METHOD FOR PREPARING CYTOLOGICAL SPECIMENS - An apparatus for processing a specimen from a fluid sample includes a first set of one or more microfluidic channels configured to deliver the sample fluid to a filter disposed on an inflatable bladder configured to transfer the specimen from the filter to a slide. The apparatus is configured to collect an approximate monolayer of particles and includes a second set of one or more microfluidic channels configured to remove fluid flowing through the filter disposed on the inflatable bladder. The apparatus also includes a pressure source, a sample container connected to the pressure source and the first set of one or more microfluidic channels, a fluid flow gauge configured to measure a fluid flow rate through the filter, and a stain source connected to the first set of one or more microfluidic channels.04-15-2010
20100093017Metabolically Competent Cell Lines - The present invention provides cell lines that have been transfected with adenovirus expression vectors so that they express at least one metabolically competent or functional cytochrome P450 enzyme. The invention also includes methods of their use, especially in toxicology screens.04-15-2010
20100093018CELLS EXPRESSING CHIMERIC PROTEINS AND ASSAYS USING SUCH CELLS - Cells expressing chimeric hepatitis C virus NS4A protein are disclosed, as are vectors and methods for preparing such cells and methods of using the cells and the chimeric proteins therein in screening assays for detecting compounds that alter the associative characteristics of NS4A proteins. Disclosed screening assays are suitable for use in high throughput screening of compound libraries to identify compounds with a exhibiting specific anti-viral activity against hepatitis C virus.04-15-2010
20100093015Spectroscopic systems and methods for classifying and pharmaceutically treating cells - A system and method to distinguish normal cells from cells having undergone a biochemical change. A pre-determined vector space is selected where the vector space mathematically describes a first plurality of reference spectral data sets for normal cells and a second plurality of reference spectral data sets for cells having undergone a biochemical change. A sample is irradiated to generate a target spectral data set based on photons absorbed, reflected, emitted, or scattered by the sample. The target spectral data set is transformed into a pre-determined vector space. A distribution of transformed data is analyzed in the pre-determined vector space. Based on the analysis, the sample is classified as containing normal cells, cells having undergone a biochemical change, and combinations thereof. The method includes treating the sample with a pharmaceutical agent prior to irradiating the sample and using the classification to assess the efficiency of the pharmaceutical agent.04-15-2010
20120122146NANOPARTICLE SCREENING METHODS EMPLOYING INSECTS WITH BLOOD BRAIN BARRIER - There is provided an insect model for determining the penetration of nanoparticles through the BBB. The model is also directed to the use of insects for studying the environmental safety of nanoparticles. The particles are administered into the hemolymf of an insect having a BBB, such as the locust, and the uptake into the brain is analysed. Thus, in this model it is possible to investigate the potential up-take into the brain of nanoparticles circulating in the blood.05-17-2012
20120122145INSECT-BASED EX VIVO MODEL FOR TESTING BLOOD-BRAIN BARRIER PENETRATION AND METHOD FOR EXPOSING INSECT BRAIN TO CHEMICAL COMPOUNDS - There is provided an ex-vivo insect screening model to accurately determine blood-brain barrier penetration of different chemical compounds in order to improve the compound screening procedures/processes in the early drug discovery process. This object offers many advantages relative to prior technologies since insect models are more reliable tools for the decision-making process than the existing in vitro models, and will speed up the drug screening process and reduce the late phase attrition rate. Moreover, it will reduce the number of mammals sacrificed during the drug discovery phase.05-17-2012
20120122144METHODS EMPLOYING INSECT MODELS FOR DETERMINING INTESTINAL ABSORPTION OF CHEMICAL COMPOUNDS - There is provided insect screening models to determine gastrointestinal absorption of different chemical compounds in vertebrates, and in particular humans, in order to improve the compound screening procedures/processes in the early drug discovery process. This offers many advantages relative to prior technologies since insect models are more reliable tools for the decision-making process than the existing in vitro models, and will speed up the drug screening process and reduce the late phase attrition rate. Moreover, it will reduce the number of mammals sacrificed during the drug discovery phase.05-17-2012
20120122142CELL DEPOSITION SYSTEM - A system for depositing cells on an analysis plate, the cells being contained in a cell suspension including a fixing agent and the cells, included, for receiving the suspension, a chamber (05-17-2012
20120122141Point Source Diffusion Cell Activity Assay Apparatus - Apparatuses and methods for determining whether a test compound solution induces cell activity, an embodiment of the method of the present invention comprising placing a test compound solution in contact with a cell suspension media containing cells, diffusing the test compound solution into the cell suspension from a point source, and detecting activity in the cells with respect to their distance from the point source. Detecting activity in the cells can involve detecting activity in a first group of the cells proximate to the point source, and detecting activity in a second group of the cells farther from the point source than the first group.05-17-2012
20120122139MICROFLUIDIC DEVICE AND HEMOGLOBIN MEASUREMENT METHOD USING THE SAME - A microfluidic device and a method for measurement of biomaterials using the same. The microfluidic device includes a microfluidic structure including: a sample chamber which receives and accommodates blood; a reagent chamber which contains a luminescent reactant; a first detection chamber which contains a first material that is positively charged; a second detection chamber which is connected to the first detection chamber, and contains a second material having a boronate moiety; and at least one channel which connects the sample chamber, the reagent chamber and the first and second detection chambers.05-17-2012
20120315663Device For Separating A Membrane From A Support12-13-2012
20120122143TECHNIQUE FOR DETERMINING MATURITY OF A CELL AGGREGATION, IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING THE TECHNIQUE, AND METHOD FOR PRODUCING A CELL AGGREGATION - An image processing program (GP) is configured to comprise a step (A05-17-2012
20090130703Methods of Detection of Changes in Cells - Methods are provided to detect changes in cells without the use of detection labels.05-21-2009
20090130702Methods and systems for providing a nutraceutical program specific to an individual animal - The present invention relates to methods and systems for providing an animal with a nutraceutical program that is specifically tailored to address the deficiencies and/or needs of the particular animal. Blood is drawn from the animal and analyzed at a lab to obtain blood test results. The blood test results are scored to obtain at least one blood test score for at least one corresponding blood parameter. If the at least one blood test score falls within a normal range but outside of an optimal range, one or more nutraceuticals needed to bring the animal within the optimal range for the at least one corresponding blood parameter are identified. A prescribed dosage amount is then calculated for at least one of the one or more identified nutraceuticals for the animal. The calculation of the prescribed dosage amount is based on at least the blood test score for the corresponding blood parameter and a deviation of the blood test score from the optimal range.05-21-2009
20090130700Device and Method for Determining the Concentration of a Substance - The invention provides a method for determining a concentration of a substance in a compartment comprising exciting an endogenous compound, or a functional part, derivative, analogue or precursor thereof, measuring the lifetime of the luminescence and/or transient absorption exhibited by said compound, functional part, derivative, analogue and/or precursor, and correlating said lifetime with the concentration of said substance.05-21-2009
20100248293SAMPLE ANALYZER AND SAMPLE ANALYZING METHOD - The present invention is to present a sample analyzer comprising: a first measurement unit; a second measurement unit; a transport unit for transporting a sample container to the first measurement unit and the second measurement unit; and a controller configured to perform operations comprising: obtaining measurement item information indicating a measurement item of the sample contained in the sample container; and controlling the transport unit so as to transport the sample container to the second measurement unit when a first measurement item and a second measurement item different from the first measurement item are indicated in the measurement item information, and controlling the transport unit so as to transport the sample container to the first measurement unit or the second measurement unit when the first measurement item is indicated and the second measurement item is not indicated in the measurement item information.09-30-2010
20100248285Method And Apparatus For The Processing And/Or Analysis And/Or Selection Of Particles, In Particular Biological Particles - Methods and apparatus are described for the processing (for example washing, incubation, etc.) of particles in which the particles suspended in a first fluid are introduced under laminar flow conditions into at least one first microchamber or first region of the same, in which a second fluid is introduced under laminar flow conditions into at least one second region of the microchamber or of a second microchamber, in such a way as not to mix with the first fluid, and in which at least one field of force acting on the particles is activated in the microchamber(s), to provoke a shift of the particles alone in a predetermined direction and to transfer the same in suspension into the second fluid; an apparatus is preferably used including at least three microchambers n microchambers arranged in sequence with each other in one direction and each connected with the microchamber immediately before it and after it with two orifices offset from each other in a direction perpendicular to the direction of sequence of the microchambers.09-30-2010
20100248284BIOSENSOR - A biosensor which comprises a substrate (09-30-2010
20100248283Portable surface plasmon resonance biosensor - A surface plasmon resonance biosensor device and system are provided. The simplicity of SPR biosensor design allows easy integration with both QCM and electrochemistry techniques, not found in current SPR biosensor devices. In some embodiments, the surface plasmon resonance biosensor device has a dual SPR/QCM sample holder which allows simultaneous detection by both surface plasmon resonance and also quartz crystal microbalance (QCM) techniques. In additional embodiments, the surface plasmon resonance biosensor device and/or the dual SPR/QCM technique can be integrated with electrochemistry techniques by incorporate reference and counter electrodes in the SPR or SPR/QCM sample holder. Methods of using the device and system to determine whether an analyte of interest is present in a sample are also provided.09-30-2010
20120231489IRIDESCENT SURFACES AND APPARATUS FOR REAL TIME MEASUREMENT OF LIQUID AND CELLULAR ADHESION - Described is a method and apparatus for determining the adhesion of an object to an iridescent surface based on the detected scattered light scattered by the interface region for the iridescent surface and the object.09-13-2012
20120231487Method for preparing an invasive test of an egg and for determining a gender of an embryo in an egg - The present invention relates to a method for preparing an invasive test on an egg, such as determining the gender of an embryo in an egg, comprising the step of: providing a passage to the interior of an egg. The present invention also relates to a method for determining the gender of an embryo in an egg.09-13-2012
20120129210Method for Assaying Inositol Hexaphosphate (IHP) - The invention relates to a method for assaying inositol hexaphosphate (IHP) in a product that can be injected in humans or animals or in a fraction of this product, in which a metal compound is added to a sample or a fraction of this product and the complexation of said metal compound with the IHP present is subsequently detected, by virtue of which the IHP present in the product or fraction thereof is assayed. The invention makes it possible to assay the IHP in a suspension or a solution, and in particular in the various compartments of a suspension of red blood cells.05-24-2012
20120129209ALIGNING CELLS ON WRINKLED SURFACE - A method is provided for preparing an aligned cell population comprising the culturing one or more cells on a surface having a texture, which texture has an average height of from about 100 nanometers to about 5 micrometers, thereby forming an aligned cell population on the textured surface. Also provided is a method to prepare the surface which method comprises the steps of: a) depositing a metal onto an unstressed or pre-stressed thermoplastic material; b) reducing the surface area of the receptive material by at least about 60%; and c) preparing the surface via lithography.05-24-2012
20120129207COMPOSITIONS AND METHODS OF FUNCTIONALLY ENHANCED IN VITRO CELL CULTURE SYSTEM - Compositions and methods described herein provide a cell culture system in which cells are in high metabolic states from the onset of the culture. Combinations of various cell culture components disclosed and employed herein allow cells to be in high metabolic states useful for drug testing immediately after the start of cell culture.05-24-2012
20110183367DEVICE AND METHOD FOR NON-INVASIVE MEASUREMENT OF THE INDIVIDUAL METABOLIC RATE OF A SUBSTANTIALLY SPHERICAL METABOLIZING PARTICLE - The present invention relates to methods and devices for non-invasive and non-disturbing measurements of metabolizing rates of substantially spherical metabolizing particles, such as an embryo, and to a method and device for controlling oxygen partial pressure at the level of the embryo. Furthermore, the invention relates to a method for regulating supply of metabolites to a substantially spherical metabolizing particle, as well as a method for selecting substantially spherical metabolizing particles of a predetermined quality. The invention is carried out in a device capable of establishing a diffusion gradient of metabolites between the substantially spherical metabolizing particle inside a compartment in the device and the environment outside the compartment. The metabolizing rate is determined based on information of the metabolite diffusion gradient.07-28-2011
20110183369PLANT INFESTING SYSTEMS AND METHODS - The present disclosure provides systems and methods for infesting the roots of a plant with larval insects. In various embodiments, an exemplary method includes injecting an egg solution into a root zone of the plant, wherein the root zone is disposed within a planting media of the plant.07-28-2011
20110183368METHOD AND APPARATUS FOR USING LIGHT EMITTING DIODES IN A GREENHOUSE SETTING - There is provided a modular LED system comprising a frame (07-28-2011
20090253159ION CHANNEL ASSAY METHODS - A method of characterizing the biological activity of a candidate compound may include exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.10-08-2009
20120164679BIOLOGICAL MICROFLUIDICS CHIP AND RELATED METHODS - A biological microfluidics chip (06-28-2012
20120164678MICROBIAL PRODUCTION OF NATURAL SWEETENERS, DITERPENOID STEVIOL GLYCOSIDES - The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.06-28-2012
20120164677METHOD AND APPARATUS FOR VIABLE AND NONVIABLE PROKARYOTIC AND EUKARYOTIC CELL QUANTITATION - A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.06-28-2012
20120164676USE OF DIAZOLIDINYL UREA FOR ANTI-CLUMPING OF BIOLOGICAL SAMPLES - The present invention provides methods for preventing clumping of cells in microfluidic devices by addition of diazolidinyl urea (DU). DU can be added to samples at the time of collection or can be added to samples post-collection. DU can also be pre-added to sample collection devices.06-28-2012
20100227358MINERALIZED THREE-DIMENSIONAL BONE CONSTRUCTS - The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.09-09-2010
20100209960METHOD FOR MEASURING MITOCHONDRIAL MEMBRANE POTENTIAL IN VERTEBRATE CELLS - The present invention relates to homogenous fluorescence-based assays for measuring mitochondrial membrane potential in vertebrate cells. The assays use an inner filter such as Brilliant Black BN to quench non-specific fluorescence. The assays are particularly suited for ultra high-throughput screening for activators of mitochondrial uncoupling proteins, chemical uncouplers, compounds with mitochondrial toxicity, and compounds which stimulate mitochondrial biogenesis.08-19-2010
20100209963PHOTO-ELECTRIC DEVICE AND METHOD FOR HIGH THROUGHPUT ACTIVATION, GUIDANCE AND PORATION OF TARGETED CELLS WITH HIGH SPATIAL RESOLUTION - The method includes the steps of generating a spatially and/or temporally localized electric field generated on the photoconductive surface, and selectively activating, guiding or porating targeted (excitable) cells at high throughput with high spatial resolution, applied for example to neurons, cardiac and muscle cells. The spatially and/or temporally localized electric field can be established using spatially and/or temporally patterning light with a diffractive element to generate the spatially localized electric field on the photoconductive surface which is sandwiched between two conductive surfaces and applying a selected voltage difference between the two conductive surfaces. The intensity of the light beam can be varied for different processes of activation, guidance or poration without causing cellular damage.08-19-2010
20100209964METHOD AND APPARATUS FOR ANALYZING SAMPLE - A method for analyzing a sample includes the step of irradiating a reaction portion of a sample BL and a reagent 08-19-2010
20100209959TUMOUR-CELL-FIXING CELLS - The present invention relates to an isolated cell capable of fixing tumour cells, expressing the CD10 protein and expressing at least one MDR protein, and to the use of this cell for screening for anti-tumour compounds.08-19-2010
20100209958METHOD OF TREATING ARTHRITIS AND PROMOTING GROWTH OF ARTICULAR CHONDROCYTES - This invention provides a new therapeutic or prophylactic agent for arthritis such as osteoarthritis. Specifically, it provides a therapeutic or prophylactic agent for arthritis such as osteoarthritis, or an agent for promoting the growth of articular chondrocyte, comprising a guanyl cyclase B (GC-B) activator as an active ingredient; or a method for inhibiting arthritis or for promoting the growth of articular chondrocyte by activating GC-B; or a method for screening an agent for promoting the growth of articular chondrocyte or an agent capable of treating arthritis using the GC-B activity as an indication.08-19-2010
20100209961MICROORGANISM CONCENTRATION PROCESS AND AGENT - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.08-19-2010
20120315662METHOD FOR THE EARLY DETECTION OF HIGH-GRADE PELVIC SEROUS CANCER - A method for early detection of high-grade pelvic serous cancers, comprising acquiring fallopian tube cells in vivo by exfoliative cytology, and examining the acquired cells for precursors of high-grade pelvic serous cancer.12-13-2012
20120315661Derivatives of 1,2-dihydro-7-hydroxyquinolines Containing Fused Rings - The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.12-13-2012
20120214191Methods and Systems for Efficient Processing of Biological Samples - Systems and methods of sample (08-23-2012
20120214192Method for Producing Antigen-specific B Cell Population - The present invention provides a method for producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen, the method comprising: culturing IgG-positive B cells together with the specific antigen in the presence of IL-21, while conferring stimulation to the IgG-positive B cells via CD40, a BAFF receptor and Fas; and screening for antigen-specific B cells specific to the specific antigen to obtain an antigen-specific B cell population comprising the IgG-positive B cells specific to the specific antigen.08-23-2012
20120214190System and method based on blood components for estimating human physiological parameters - A system and method based on blood components for estimating human general physiological parameters comprises determining the predetermined value of general physiological parameters indicative of illness and disease to monitor health status at different time points on the basis of basic physiological parameters indicated as the percentage of a particular cytokine-producing cells in a type of blood cells in a human peripheral blood sample without in vitro culture and with two-stage in vitro cultures in the absence and presence of microbial stimulation after being taken from the subject.08-23-2012
20120214189Devices and Methods for Pharmacokinetic-Based Cell Culture System - Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.08-23-2012
20120214188LIGHT-ACTIVATED ION CHANNEL MOLECULES AND USES THEREOF - The invention, in some aspects relates to compositions and methods for altering cell activity and function and the use of light-activated ion channels (LAICs).08-23-2012
20120135446Microfluidic Device and Related Methods - A combinatorial microenvironment generator is configured for the generation of arbitrary, user-defined, steady-state, concentration gradients with negligible to no flow through the growth medium to perturb diffusion gradients or cellular growth. More importantly, the absolute concentrations and/or gradients can be dynamically altered upon request both spatially and temporally to impose tailored concentration fields for in-situ stimulus studies. Here, diffusion occurs via an array of ports, each of which can be an independently controlled source/sink. Together, the array of ports establishes a user-defined, 3D concentration profile. Useful methods related to this device are also provided.05-31-2012
20120135452MICROFLUIDIC DEVICE FOR PHARMACOKINETIC-PHARMACODYNAMIC STUDY OF DRUGS AND USES THEREOF - A microfluidic device for culturing cells, termed a microscale cell culture analog (μCCA), is provided. The microfluidic device allows multiple cell or tissue types to be cultured in a physiologically relevant environment, facilitates high-throughput operation and can be used for drug discovery. The microfluidic device uses gravity-induced fluidic flow, eliminating the need for a pump and preventing formation of air bubbles. Reciprocating motion between a pair of connected reservoirs is used to effect the gravity-induced flow in microfluidic channels. Bacterial contamination is reduced and high throughput enabled by eliminating a pump. The microfluidic device integrates a pharmacokinetic-pharmacodynamic (PK-PD) model to enable PK-PD analyses on-chip. This combined in vitro/in silico system enables prediction of drug toxicity in a more realistic manner than conventional in vitro systems.05-31-2012
20120135451Method and Apparatus for Evaluation of Toxicity - The present invention pertains to a method for evaluation of the toxicity of compounds comprising the use of spermatozoa. The inventions also relates to a high throughput system for the toxicity evaluation.05-31-2012
20120135450SUBSTRATES, DEVICES, AND METHODS FOR QUANTITATIVE LIQUID CRYSTAL ASSAYS - The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.05-31-2012
20120135448METHODS AND DEVICES FOR THE FABRICATION OF 3D POLYMERIC FIBERS - The present invention provides methods and devices for the fabrication of 3D polymeric fibers having micron, sub-micron, and nanometer dimensions, as well as methods of use of these polymeric fibers.05-31-2012
20100136603 KIT AND A METHOD FOR EVALUATING TOXICITY USING SPORE RELEASE BY THE GREEN ALGA ULVA - The present invention relates to a kit and a method for evaluating toxicity of a waterbody sample by a spore release of a green alga 06-03-2010
20120135447NUCLEIC ACIDS ENCODING T2R, A NOVEL FAMILY OF TASTE RECEPTORS - The invention provides isolated nucleic acid and amino acid sequences of taste cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste cell specific G-protein coupled receptors.05-31-2012
20120135445CELL TRANSPORT SYSTEM - The present invention relates to a system for cell transport Said system allows the transport of cells, assuring their integrity and viability during the entire transport process. It consists of a system suitable for a wide variety of formats which allows a broad range of technical applications of the system The system of the invention allows providing ready-to-use cells, without the cells having to be manipulated before they are used by technical experts in cell biology The invention particularly relates to an agarose plus agarase mixture covering or enveloping, depending on the format of the selected transport system, the cell culture, protecting it during the transport process, as well as to the methodology of cell recovery of the cells transported in the system.05-31-2012
20100173348Method of evaluating visceral fat accumulation, visceral fat accumulation-evaluating apparatus, visceral fat accumulation-evaluating method, visceral fat accumulation-evaluating system, visceral fat accumulation-evaluating program, recording medium, and method of searching for prophylactic/ameliorating substance for visceral fat accumulation - According to the method of evaluating visceral fat accumulation of the present invention, amino acid concentration data on concentration values of amino acids in blood collected from a subject to be evaluated is measured, and a visceral fat accumulation condition in the subject is evaluated based on the measured amino acid concentration data of the subject.07-08-2010
20100173350Method for discriminating between benign and malignant prostate tumors - The method for discriminating between benign and malignant prostate tumors relates to analyzing samples of blood, urine and tissue by fluorescence spectroscopy in order to detect the presence of naturally occurring molecules in the fluids and tissue that serve as biomarkers indicative of cancer in the human body. The analysis can be carried out based on fluorescence emission spectra, fluorescence excitation spectra and synchronous (emission and excitation) spectra of bio-samples. The detection, diagnosis, and follow-up and also discrimination between malignant and benign prostate tumors may be made by comparison of ratios of fluorescence emissions and/or excitation intensities of tryptophan, tyrosine, elastin, collagen, bile pigments, NADH, flavins and various species of porphyrins.07-08-2010
20100173352METHOD FOR PREPARING CELLS FOR ENGRAFTMENT - The present invention relates to an in vitro method for preparing progenitor cells having an increased adhesivity, wherein progenitor cells are contacted with an agonist of the CD47/IAP receptor thereby yielding progenitor cells presenting an increased adhesivity.07-08-2010
20100173351CARDIAC REENTRY MODEL CHIP AND APPARATUS AND METHOD FOR EVALUATING DRUG USING THE CARDIAC REENTRY MODEL CHIP - A chip has been developed that can accurately measure cell potential and cell morphology on a single cell basis. The chip also constitutes a cardiac model that comprises a closed loop whereupon cardiomyocytes and fibroblasts are suitably dispersed and arranged, and that can evaluate the effects of a drug thereon. An in vitro cardiac reentry model chip is fabricated by constructing a closed loop comprising cardiomyocytes and fibroblasts arrayed on transparent electrodes formed on a transparent substrate by using a constitution where single cells are enclosed in a specific spatial configuration. A pulse wave of a random cardiomyocyte or a specific cardiomyocyte is propagated on both sides of the loop, and the pulsation status of the cells in the loop is detected electrically. A drug is applied to this cardiac reentry model chip, and the benefit or toxicity of the drug to cardiomyocytes is evaluated by measuring the cell potentials of individual cells.07-08-2010
20100173347STABILIZED GOLD NANOPARTICLES AND METHODS OF MAKING THE SAME - The present disclosure relates to water-soluble stable gold nanoparticles (AuNPs) and methods for making the same. The present disclosure also includes the use of AuNPs, for example, in biological, medical and environmental assays for the detection of analytes, as well as biological and medical imaging.07-08-2010
20100173345DETECTION OF BLOOD PLASMA AMYGDALIN OF DISSIPATING BLOOD STASIS BOTANICAL - A detection method of blood plasma amygdalin of dissipating blood stasis botanical is disclosed. The method includes: (1) adding 2-5 mol/L phosphoric acid to plasma of mammalian administered dissipating blood stasis botanical and mixing the solution of plasma-phosphoric acid (1:2-3, v/v), applying the solution to small column of Waters Oasis HLB activated by methanol and water; after leaching by water and 80-100% methanol and eluting by 0.2-1% ammonia-methanol, drying and enriching the eluent at 25-30° C.; and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C07-08-2010
20120252051TLR8 TRANSGENIC ANIMALS - Provided herein are human Toll-like receptor 8 (TLR8)-expressing transgenic animals and methods of use thereof.10-04-2012
20120171715SEMICONDUCTOR BIOSENSORS - The present application relates to semiconductor devices, in particular to a device for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time, comprising (a) at least one discrete area comprising a high electron mobility transistor (HEMT); and (b) non-excitable cells attached to said HEMT (HEMT element) for example, fibroblasts, HEK, CHO cell lines, keratinocytes, etc. Preferably, the HEMT is an AlGaN/GaN FET. Accordingly, the device can be applied in uses and methods for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time. Likewise, the device can be applied for screening compounds that reverse, protect from and/or shield cells from external stimuli which cause damage to cells. Also, kits comprising the device are disclosed.07-05-2012
20120171714METHOD AND PARTICLE ANALYZER FOR MEASURING A LOW CONCENTRATION PARTICLE SAMPLE - A method and particle analyzer for measuring a low concentration particles sample disclosed.07-05-2012
20120171716Measurement of Mitochondrial Membrane Potential to Assess Organ Dysfunction - The present invention relates to the field of organ transplantation. More specifically, the present invention provides methods for predicting organ function after transplantation. In certain embodiments, the method comprises measuring mitochondrial membrane potential from a biopsy sample from the donor organ. The present invention is also applicable to organ dysfunction in general. More specifically, the methods of the present invention may be useful in formulating prognoses for patients with acute or chronic organ dysfunction due to ischemia, infection, drug injury or age. In this rapid procedure, only small samples of tissue are required, enabling the clinical application of mitochondrial function previously thought impractical.07-05-2012
20120077220HARVESTED SAMPLE PREPARATION PERSONAL BOX AND SYSTEM AND METHOD OF HARVESTED SAMPLE PREPARATION - A harvested sample preparation system includes an operation isolator 03-29-2012
20120077219VIBRATING MICROPLATE BIOSENSING FOR CHARACTERISING PROPERTIES OR BEHAVIOUR OF BIOLOGICAL CELLS - There is described a method of characterising a property or behaviour of at least one biological cell. The method comprises the steps of: providing a microplate; submerging at least one surface of the microplate in a cell culture medium such that at least one biological cell to be characterised is in contact with the microplate; vibrating the microplate; providing a plurality of mutually spaced sensors coupled to the microplate; obtaining a respective sensory data time series from each sensor during vibration of the microplate, the microplate and the sensors being arranged such that the obtained sensory data time series are not independent from one another; and processing the sensory data time series so as to characterise a property or behaviour of the at least one biological cell. A corresponding system for characterising a property or behaviour of at least one biological cell is also described.03-29-2012
20120077217BUFFY COAT TUBE AND FLOAT SYSTEM AND METHOD - A system for separating and axially expanding the buffy coat is provided that includes a flexible sample tube and a rigid separator float. The sample tube has a sidewall with a first cross-sectional inner diameter. The float includes a main body portion and one or more support members protruding from the main body portion. The float has a cross-sectional diameter less than that of the first cross-sectional inner diameter when the sample tube is expanded. The main body portion of the float and the sidewall define an annular volume therebetween. The support members traverse said annular volume to produce one or more analysis areas. During centrifugation, centrifugal force enlarges the diameter of the tube to permit density-based axial movement of the float. The centrifugal force is reduced to return the tube to its first diameter, thereby capturing the float and trapping the buffy coat constituents in the analysis area.03-29-2012
20120252056IMAGING METHOD AND SYSTEM USING SUBSTRATE FUNCTIONALIZATION - An imaging system for analyzing fluorescent molecules in a sample, including a confocal microscope device, has a support in contact with at least a portion of the sample. In the system, the support surface in contact with the sample is functionalized so as to reduce the observation volume of the microscope on the surface in the axial direction. The present disclosure also relates to various uses of such a system as well as to a method for analyzing fluorescent molecules in a sample, the method being implemented by such a system.10-04-2012
20120252055ARRANGEMENT AND METHOD USING MICROSENSORS FOR MEASURING CELL VITALITIES - An arrangement and a method measures cell vitalities with a sensor array. The sensor array is formed on a surface of a semiconductor chip. The semiconductor chip has integrated circuits and an integrated circuit is associated with each sensor of the sensor array, for processing the measurement signals of the respective sensor. The integrated circuits are formed in the semiconductor chip spatially in each case below the associated sensor and neighboring sensors of the sensor array have a centre-to-centre in the range of micrometers. The pH and/or pO10-04-2012
20110201045METHOD AND APPARATUS FOR PERFORMING HEMATOLOGIC ANALYSIS USING AN ARRAY-IMAGING SYSTEM FOR IMAGING AND ANALYSIS OF A CENTRIFUGED ANALYSIS TUBE - A method and device for analyzing a hematologic sample centrifuged within a capillary tube is provided. The device includes a tube holder, a sample imaging device, a processor, and a sample data display. The sample imaging device is operable to create a digital image of the sample within a region of the tube. The region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube in the region where the float resides after centrifugation. The sample imaging device is operable to produce signals representative of the image. The processor is adapted to produce information relating to bands of interest within the image based on the signals from the sample imaging device. The sample data display is adapted to display the results therefrom and/or a digital image of the sample within the region.08-18-2011
20110207165SMALL SCALE SHAKER FLASK CULTIVATION - The present invention relates to a method for the cultivation of a mammalian cell in a cultivation medium of a cultivation vessel comprising adjusting the rate of a rotational movement of the cultivation vessel depending on the viable cell density in the cultivation medium.08-25-2011
20100062473IMMUNITY EVALUATION METHOD, IMMUNITY EVALUATION APPARATUS, IMMUNITY EVALUATION PROGRAM AND DATA RECORDING MEDIUM HAVING THE IMMUNITY EVALUATION PROGRAM STORED THEREIN - An immunity evaluation method for evaluating immunity by using immune cell markers for immune cells contained in sampled blood comprises the step of determining an evaluation value for each of two or more selected kinds of immune cell markers based on the individual immune cell markers contained in the sampled blood, the step of adding the evaluation values so obtained for the at least two selected kinds of immune cell markers, and the step of evaluating the immunity from the results of the adding.03-11-2010
20100062479METHOD AND DEVICE - A method of providing suspended cells (03-11-2010
20100055729SENSOR DEVICE - Sensor devices and compositions can be used for detecting the presence of a target substance in a medium. The sensor devices and compositions can be configured to include at least one nanosensor through a high concentration of nanosensors that interact with the target substance to provide a detectable signal as an indication of such an interaction. The interaction is evidence that the medium includes the target substance. Various types of target substances can be detected with the sensor device, including inorganic molecules, organic molecules, atoms, ions, polypeptides, polynucleotides, cells, derivatives thereof, and combinations thereof.03-04-2010
20120214193METHOD FOR MEASUREMENT OF FLUORESCENCE INTENSITY OF VOLTAGE-SENSITIVE FLUORESCENT DYE - An object of the present invention is to provide a method for increasing the change in the fluorescent intensity as emitted from potential-sensitive fluorochromes depending on a potential or ionic strength change. Another object of the present invention is to measure the changes in the activity potentials of ES cell- or iPS cell-derived cardiomyocytes that have heretofore been impossible to measure.08-23-2012
20120315660METHODS AND APPARATUS FOR IMPROVING IN VITRO MEASUREMENTS USING BOYDEN CHAMBERS - Apparatus and methods to improve the Boyden chamber used in cellular biological measurements, allowing quantitative optical microscopy of biological cells in situ without using fluorescent probes or optical staining. In the preferred embodiment, a thin porous membrane separating top and bottom reservoirs includes an array of precisely positioned micropores pores manufactured using a laser-based photo-machining (ablation) process. The membrane may be composed of polyethylene terephthalate (PET), polycarbonate, polyimide, polyether ether ketone (PEEK) or other appropriate material. The pores formed in the membrane may have diameters in the range of 1 to 15 microns and spaced apart at a distance ranging from 10 to 200 microns. A plurality of upper and lower reservoirs may be provided to form a multi-well plate. The invention finds application in a wide range of potential biological applications where Boyden chamber geometries are currently used including co-culture studies, tissue remodeling studies, cell polarity determinations, endocrine signaling, cell transport, cell permeability, cell invasion and chemotaxis assays.12-13-2012
20100047846ENDOGENOUS AND NON-ENDOGENOUS VERSIONS OF HUMAN G PROTEIN-COUPLED RECEPTORS - The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor for which the endogenous ligand is unknown (“orphan GPCR receptors”), and most particularly to mutated (non-endogenous) versions of the human GPCRs for evidence of constitutive activity.02-25-2010
20100047845METHOD OF REDUCING CURVATURE IN A MENISCUS OF LIQUID MEDIUM - The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.02-25-2010
20100047844DIAGNOSTIC MARKER FOR FABRY DISEASE - The present invention is in the field of Fabry disease and concerns a pathogenic factor allowing diagnosis of Fabry disease. In particular lyso-ceramide trihexosamide (lyso-CTH) has been found to function as a diagnostic marker for Fabry disease.02-25-2010
20100047843COMPOSITIONS AND METHODS FOR ENHANCING THE GROWTH OF MOUSE EMBRYONIC STEM CELLS - Compositions and methods are provided which improve the growth rate, self-renewal potential and capacity of germ line transmission of mouse embryonic stem cells.02-25-2010
20100047847Methods for diagnosing ovarian cancer - The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying ovarian cancer status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as ovarian cancer, ovarian cancer of low malignant potential, benign ovarian disease or other malignant condition. The biomarkers can be detected by SELDI mass spectrometry, immunoassay, or other means.02-25-2010
20120219984METHOD FOR THE ANAEROBIC TREATMENT OF A WASTEWATER AND ASSOCIATED DEVICE - The invention relates to a device and a method for anaerobic treatment of wastewater in a biological reactor (08-30-2012
20120219981MECHANICAL STRETCHING DEVICE - The present invention is directed to a mechanical stretching device, comprising a stretchable substrate comprising at least one stretching material area within which a stretching material is placeable, and two engagement areas being located at opposite ends of the stretchable substrate, respectively, two movable elements, each of which comprising an engagement portion, wherein each of the engagement portions is capable of engaging with one of the engagement areas, and two motors, each of which being configured to drive one of the movable elements, wherein the movable elements are movable by the motors such that the engagement portions cause, after having engaged with the engagement areas, either one end or both ends of the stretchable substrate to be stretched, wherein the ends of the stretchable substrate are to be stretched along opposite directions with respect to each other.08-30-2012
20120219980SYSTEM AND METHOD FOR PIPETTING GUIDANCE - Embodiments are described wherein systems and methods for assisting with pipette-based substance transport between two or more trays is disclosed. A controller may be operatively coupled to each of a first matrix of lights and a second matrix of lights and configured to selectively and discretely illuminate one or more of a first matrix of wells and a second matrix of wells subject to a predetermined instruction set contained on a memory device operatively coupled to the controller. The predetermined instruction set may be configured to direct a pipette operator, by discretely illuminating groupings of wells in the first and second matrices of wells, to sample substances from various wells of the first matrix of wells and dispose of them in various wells of the second matrix of wells.08-30-2012
20100273206DIAGNOSTIC IN VITRO METHOD FOR ASSESSING VON WILLEBRAND DISEASE AND INCREASED BLEEDING RISK ASSOCIATED WITH VON WILLEBRAND DISEASE AND ACQUIRED OR CONGENITAL DISORDERS OF PLATELET FUNCTION - The invention relates to an in-vitro method for diagnosing Von Willebrand Disease (VWD) and an increased bleeding risk associated with Von Willebrand Disease and/or acquired or congenital platelet function defects that reduce the interactions of Von Willebrand Factor (VWF) with platelets. The in-vitro method of the invention may also be used to diagnose further bleeding risks. The test is suitable for use as a screening test based on whole blood and has the additional benefit of being suitable as a point of care test. The method involves the incubation of a sample containing platelets and hemostasis factors with an activator of platelet aggregation and the measurement of the viscoelastic change after inducing coagulation, e.g., by means of thromboelastography (TEG).10-28-2010
20120219983 METHOD FOR OBTAINING STRUCTURAL AND FUNCTIONAL INFORMATION ON PROTEINS, BASED ON POLARIZATION FLUORESCENCE MICROSCOPY, AND A DEVICE IMPLEMENTING SAID METHOD - The invention pertains to a method of obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, which comprises subjecting a protein tagged with a fluorophore to two- or multi-photon fluorescence microscopy, whereas the observed protein is irradiated with a laser beam with light of at least two different polarizations, which excites the fluorescence of the fluorophore, and wherein information on localization, intensities and polarizations of the fluorescence excited by the different polarizations of the excitation laser beam is used to identify, localize and quantify anisotropy of absorption and/or fluorescence, which information is then used to infer structural and functional properties of proteins. An example of a device for obtaining structural and functional information on proteins, based on polarization fluorescence microscopy, comprises a modulator (P) for rapid modulation of the excitation beam (08-30-2012
20120219982METHODS FOR SELECTING ACTIVE AGENTS FOR CANCER TREATMENT - The present invention provides methods for individualizing chemotherapy, and particularly methods for individualizing neoadjuvant chemotherapy. The present invention provides methods for predicting a cancer patient's response to neoadjuvant chemotherapy, including assessing the probability of a positive response upon treatment with candidate agents prior to surgery. In various aspects, the invention involves culturing a monolayer of malignant cells from an explant of a patient's biopsy specimen, such as a transcutaneous biopsy-sized specimen, and testing the malignant cells for resistance or sensitivity to one or a plurality of candidate agents for neoadjuvant therapy. In other aspects, the invention provides methods for accurately scoring and interpreting such assays, and discloses in vitro chemoresponse results that are predictive of a patient's pathological complete response (pCR) upon receiving the corresponding treatment regimen.08-30-2012
20120178119METHOD FOR MEASURING AUTOPHAGY - This invention relates to a method for measuring autophagy in cells, comprising using, as a probe reagent, a single fluorescent protein, to measure a change in fluorescence properties of the fluorescent probe reagent depending on pH changes associated with autophagy, thereby determining the presence or activity of autophagy, wherein the single fluorescent protein is resistant to degrading enzyme activity in the lysosome or vacuole of the cell, it is not denatured or inactivated under acidic to neutral pH environment, and it is capable of changing excitation spectra or fluorescence spectra when located under the environments of acidic region and neutral region.07-12-2012
20120178121METHOD TO CHARACTERIZE BLOOD AND RED BLOOD CELLS VIA ERYTHROCYTE MEMBRANE FRAGILITY QUANTIFICATION - An apparatus and method for quantifying RBC fragility via stress-induced hemolysis and subsequent optical and computational analysis. While directed toward applications in blood quality control, the technology could have application in diagnosis. The apparatus comprises: a hemolysis unit; an optical analysis unit; and a computation unit. Similarly, the associated process comprises: a hemolysis step; an optical analysis step; and a computation step.07-12-2012
20110104734NOVEL STRATEGY TO REDUCE LACTIC ACID PRODUCTION AND CONTROL PH IN ANIMAL CELL CULTURE - The present disclosure provides a method for culturing cells in exogenous lactic acid. Certain aspects of the present disclosure include the production of recombinant proteins, such as antibodies and fragments thereof. Certain aspects of the present disclosure also relate to methods of controlling lactic acid production, pH stability and osmolality in cell culture.05-05-2011
20100273205Magnetic resonance imaging contrast agents - This invention relates to novel magnetic resonance imaging contrast agents, and methods of making and use thereof.10-28-2010
20100273204METHODS FOR MONITORING THE EFFICACY OF ANTI-IL-2R ANTIBODIES IN MULTIPLE SCLEROSIS PATIENTS - The use of HLA-DR10-28-2010
20100009399Novel population of multipotent cardiac precursor cells derived from human blastocysts derived stem cells - A novel population of multipotent cardiac precursor (MCP) cells derived from human blastocysts derived stem cells is disclosed, methods for the preparation thereof and use of the cells for in vitro testing. Basement cells derived from hBS cells are also disclosed and method for the preparation of MCP cells from basement cells. The MCP cells have the following characteristics 01-14-2010
20100009401METHOD OF EVALUATING COLORECTAL CANCER, COLORECTAL CANCER-EVALUATING APPARATUS, COLORECTAL CANCER-EVALUATING METHOD, COLORECTAL CANCER-EVALUATING SYSTEM, COLORECTAL CANCER-EVALUATING PROGRAM AND RECORDING MEDIUM - According to the method of evaluating colorectal cancer of the present invention, amino acid concentration data on the concentration value of amino acid in blood collected from a subject to be evaluated is measured, and a colorectal cancer state in the subject is evaluated based on the concentration value of at least one of Arg, Cys, Orn, Trp, Glu, ABA, Val, Phe, Leu, Gln, Ile and His contained in the measured amino acid concentration data of the subject.01-14-2010
20100009400METHODS OF STUDYING A BIOMARKER, AND METHODS OF DETECTING A BIOMARKER - Embodiments of the present disclosure provide for methods of studying (e.g., detecting, localizing, and/or quantifying) biomarker(s) and the like.01-14-2010
20090061475Methods for Identifying Compounds for the Treatment of Type 1 Diabetes - The present invention provides methods of identifying candidate compounds for the treatment of type I diabetes comprising contacting pancreatic β cells with an amount of apolipoprotein CIII (“apoCIII”) effective to increase intracellular calcium concentration, in the presence of one or more test compounds, and identifying those test compounds that inhibit an apoCIII-induced increase in intracellular calcium concentration in the pancreatic β cells. The present invention also provides methods for treating patients with type I diabetes comprising administering to the patient an amount effective of an inhibitor of apoCIII to reduce apoCIII-induced increase in intracellular calcium concentration in pancreatic β cells.03-05-2009
20090061473Measurement of Carbonaceous Particles in Biological Samples - Disclosed is a method of quantitative estimation of carbonaceous particles in biological samples such as biological cells and tissues.03-05-2009
20090061474CELLS AND METHODS FOR ESTIMATION OF EFFECTS ON NEUROLOGICAL DYSFUNCTION - The invention provides neuron-derived cells obtained by transfecting a receptor-expressing nucleic acid having an aryl hydrocarbon receptor gene, wherein outgrowth of neurites is not observed without adding a substance for the aryl hydrocarbon receptor, and outgrowth of neurites is observed by adding the substance for the aryl hydrocarbon receptor. The invention also provides a method for determining the presence of neurotoxicity of a test substance, a method for acquiring a marker for determining the presence of neurotoxicity of the test substance, a method for acquiring a marker for neurological dysfunction, and a method for determining the effect of the test substance on neurological dysfunction using such cells.03-05-2009
20090325214NUCLEAR MATRIX PROTEIN ALTERATIONS ASSOCIATED WITH COLON CANCER AND THEIR USE AS MARKERS FOR COLORECTAL CANCER - CCSA-2 levels in serum samples from individuals suffering from colorectal cancer can be used gauging the progression of a colorectal cancer condition. In particular, the progression of a colorectal cancer condition is gauged by comparing the level of serum CCSA-2 from individuals having colorectal cancer to predetermined CCSA-2 levels that correlate to the state of advancement of a cancer condition. Additionally, serum CCSA-2 levels can be used by a clinician to gauge the efficacy of a colorectal cancer treatment.12-31-2009
20120225445SEBOCYTES, SEBOCYTE CELL LINES AND APPLICATIONS THEREOF - Adipose cells (sebocytes) are described, The invention especially relates to sebaceous gland cells and to a sebaceous gland cell line with the property of being continuously grown over many sub-cultures. The sebocytes are excellently suited for useful applications.09-06-2012
20100291614IN VITRO METHOD FOR DETERMINING THE RISK OF AND DIAGNOSIS OF AN ARTERIAL VASCULAR DISORDER - An in vitro method is disclosed for (i) determining the risk to an individual of developing an arterial vascular disorder, or (ii) diagnosing an arterial vascular disorder. In at least one embodiment, the method includes at least the following: (a) determining at least one biomarker characteristic of a vascular disorder and/or an early stage of an inflammation in a biological sample of an individual, (b) determining the state of a vessel displayed using an imaging method, wherein the information obtained in (a) and (b) is correlated with (i) or (ii).11-18-2010
20100291613Method of Positioning an Organic, Biological and/or Medical Specimen - The invention relates to a method of positioning an organic, biological and/or medical specimen in a desired partial region of a specimen carrier, comprising the steps arrangement of a gel in a partial region of the specimen carrier, polymerisation or gelification of the gel, by means of which a polymerised gel is obtained, whereby the polymerised gel at least partially, in particular horizontally, delimits the desired partial region and introduction of the specimen into the specimen carrier, in particular into the desired partial region of the specimen carrier.11-18-2010
20100291611Assays - A device includes a substrate that defines, at least in part, a microfluidic network including an inlet in communication with a first detection zone and with a second detection zone. A cobalt reagent and a nickel reagent are disposed within the microfluidic network. First electrodes are in communication with the first detection zone and second electrodes arc in communication with the second detection zone. The device is configured to receive a blood derived sample introduced to the inlet, partition the blood sample into first and second blood sample portions, form a first mixture including at least some of the first blood sample portion and at least some of the cobalt reagent, and form a second mixture including at least some of the second blood sample portion, at least some of the cobalt reagent and at least some of the nickel reagent.11-18-2010
20100291610Regulating Stem Cells - A composition of matter is provided, comprising a population of cultured cells that comprises a sub-population of cells that both stain as CD31Bright and demonstrate uptake of Ac-LDL+, and secrete IL-8. A method is also provided, comprising stimulating in vitro an initiating cell population (ICP) of at least 5 million cells that have a density of less than 1.072 g/ml, wherein at least 1% of the cells of the ICP is CD34+CD45−/Dim, and at least 25% of the cells of the ICP are CD31Bright, to differentiate into a progenitor/precursor cell population (PCP). Other embodiments are also described.11-18-2010
20100291616NOVEL GENES, COMPOSITIONS, KITS, AND METHODS FOR IDENTIFICATION, ASSESSMENT, PREVENTION AND THERAPY OF CERVICAL CANCER - The invention relates to newly discovered nucleic acid molecules and proteins associated with cervical cancer including pre-malignant conditions such as dysplasia. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human cervical cancers are provided.11-18-2010
20100291615System and method for automatically venting and sampling a culture specimen container - Apparatus for automated venting and/or sampling of a specimen container having a closure sealing the interior of the specimen container from the environment is disclosed. The apparatus includes a rack holding the specimen container; a venting and/or sampling device having a needle, a chamber in fluid communication with the needle and a port in fluid communication with the chamber; a robotic transfer mechanism moveable relative to the rack; a sample removal apparatus attached to the robotic transfer mechanism having gripping features for gripping the venting device. The sample removal apparatus and robotic transfer mechanism are moveable relative to the specimen container so as to automatically insert the needle of the venting device through the closure of the specimen container to thereby vent the interior of the specimen container and obtain equilibrium between the interior of the specimen container and the atmosphere and withdraw a portion of the sample from the specimen container into the venting and/or sampling device.11-18-2010
20120264159SCREENING SYSTEM FOR MODULATORS OF HER2 MEDIATED TRANSCRIPTION AND HER2 MODULATORS IDENTIFED THEREBY - This invention pertains to the development of a screening system to identify (screen for) HER2 promoter silencing agents. Such agents are expected to be of therapeutic value in the treatment of cancers characterized by HER2 amplification/upregulation. In addition, this invention pertains to the discovery that histone deacetylase (HDAC) inhibitors like sodium butyrate and trichostatin A (TSA), in a time and dose dependent fashion can silence genomically integrated and/or amplified/overexpressing promoters, such as that driving the HER2/ErbB2/neu oncogene, resulting in inhibition of gene products including transcripts and protein, and subsequent production of tumor/cell growth inhibition, apoptosis and/or differentiation. In another embodiment, this invention provides novel SNPs associated with the coding region of the ERbB2 proto-oncogene. The SNPs are indicators for altered risk, for developing ErbB2-positive cancer in a mammal.10-18-2012
20120225448Substrate with Photo-Controllable Cell Adhesion Property, Method for Analyzing and Fractionating Cells, and Device for Analysis and Fractionation of Cells - When cells are analyzed, fractionated, and incubated while keeping the cells alive, real-time operations can be performed more easily and the cells can be incubated while removing unnecessary cells from the incubated cells to purify the cells being incubated. Furthermore, desired cells are separated through analysis from the incubated cells, and the purity, recovery, and viability of the cells are heightened. Use is made of a substrate having photo-controllable cell adhesion properties, the substrate comprising a transparent base and, formed thereon, a film of a material which has photo-controllable cell adhesion properties and has been obtained by bonding a cell-adhesive material to a cell-non-adhesive material through photo-dissociable groups. Cell images are detected and analyzed to obtain information about the location of desired cells. On the basis of the information, a space is formed between cells and the material having photo-controllable cell adhesion properties is cut, by means of second light irradiation. Meanwhile, by means of first light irradiation, the surface of the substrate is changed from a cell-adhesive surface to a cell-non-adhesive surface, thereby separating the cell(s) from the substrate. Thus, cells can be analyzed and fractionated while keeping the cells alive.09-06-2012
20120225447COMPOSITIONS AND METHODS FOR QUANTITATIVELY MONITORING LIPIDS - Provided herein are fluorescent lipid binding proteins (FLBPs). The FLBPs comprise a lipid binding domain linked to a fluorophore, whrereby the fluorophore's fluorescence emission undergoes a spectral change upon lipid binding. Said fluorophore is selected from the group consisting of 2-dimethylamino-6-acyl-naphthalene (DAN) and RED fluorophore and said lipid binding protein is selected from the group consisting of ENTH domain of epsin 1, C2 domain of bovine lactadherin, C1B domain of protein kinase C-gamma, C2 domain of cytosolic phospholipase A2-beta, and PH domain of Bruton's tyrosine kinase PH.09-06-2012
20120225446PREPARATION OF THIN LAYERS OF A FLUID CONTAINING CELLS FOR ANALYSIS - Apparatus for producing thin layers of a fluid sample for analysis, has a two dimensional array of analysis chambers (09-06-2012
20120258486CALCIUM-BINDING PHOTOPROTEIN, GENE ENCODING THE SAME, AND USE THEREOF - A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.10-11-2012
20120190058METHOD FOR EVALUATING CELL POPULATIONS - The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.07-26-2012
20120190055MOLECULE BIOMARKERS OF AUTISM - This invention provides methods and biomarkers for diagnosing autism by identifying cellular metabolites differentially produced in autistic patient samples versus non-autistic controls. Methods for identifying a unique profile of metabolites present of secreted in brain tissue, cerebrospinal fluid, plasma, or biofluids of autistic samples are described herein. The individual metabolites or a pattern of secreted metabolites provide metabolic signatures of autism, which can be used to provide a diagnosis thereof.07-26-2012
20120190059METHODS FOR OBTAINING HEPATOCYTES, HEPATIC ENDODERM CELLS AND HEPATIC PROGENITOR CELLS BY INDUCED DIFFERENTIATION - The present invention discloses a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells (iPS cells) into hepatocytes, a method for inducing the differentiation of embryonic stem cells or induced pluripotent stem cells into hepatic endoderm cells, and a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells into hepatic progenitor cells. The present invention also provides the hepatocytes, hepatic endoderm cells and hepatic progenitor cells obtained by above methods, and the uses of these cells.07-26-2012
20120190057Method for the qualitative and/or quantitative analysis of tumour cells - A method for qualitative and/or quantitative analysis of tumor cells, including the following steps: a) generating a dye complex from an aminocoumarin and cyclodextrin; b) mixing the tumor cells with the dye complex prepared in step a); c) incubating the cells with the dye complex prepared in step a); d) analyzing the tumor cells by means of fluorescence microscopy and/or fluorescence spectrometric analysis.07-26-2012
20120329085FLUORESCENT PROBES FOR REACTIVE SULFUR SPECIES - The invention provides reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells.12-27-2012
20090017488Methods for Identifying Modulators of Ion Channels - The invention provides methods for identifying modulators of ion channels without the use of recombinant cell lines over-expressing the ion channel proteins or the use of detection labels.01-15-2009
20090017486METHOD AND TEST KIT FOR THE IDENTIFICATION OF COMPOUNDS SPECIFICALLY MODULATING THE ACTION OF CORTICAL AXO-AXONIC CELLS - The present invention provides a method for identifying an agent capable of specifically modulating or eliminating the action of axo-axonic cells, an experimental kit for performing the said method and compounds identifiable by the said method, as well as methods for the treatment or prevention different neurological symptoms by modulating the activity of axo-axonic cells.01-15-2009
20090017483Analytical instrument having improved arrangement of reagent section and analytical method - The present invention relates to an analytical instrument (01-15-2009
20110124036METHOD FOR MEASUREMENT OF PHYSIOLOGICALLY ACTIVE SUBSTANCE DERIVED FROM ORGANISM AND MEASUREMENT APPARATUS - Disclosed is a measurement method which can largely reduce the time required for the detection of a physiologically active substance derived from an organism (e.g., an endotoxin, β-D-glucan) or the determination of the concentration of the physiologically active substance by utilizing the reaction between the physiologically active substance and LAL. Also disclosed is a measurement apparatus utilizing the measurement method. An incident light from a light source is focused onto a sample and delivered to the sample to cause the bombardment with coagulin which is a final product of a protease cascade (i.e., a coagulin monomer) and an extremely fine aggregate which is produced by the aggregation of the coagulin monomers (i.e. a coagulin aggregate), thereby generating a scattered light. The scattered light is detected by a light-receiving element. The concentration of the endotoxin can be determined based on the initial rate of increase in the scattered light detected.05-26-2011
20110124035DEVICE FOR EXPOSING A SENSOR TO A CELL CULTURE POPULATION IN A BIOREACTOR VESSEL - Devices and methods for exposing a sensor to a cell culture or microbial population are disclosed. In one embodiment, a sensor well for use with a bioreactor vessel includes a sheath; a sensing element disposed on or in a portion of the sheath; a signal transmitter disposed within at least a portion of the sheath and configured to provide signals to and/or receive signals from the sensing element and provide signals to and/or receive signals from a sensor controller; a connector configured to attach the sensor well to a portion of a bioreactor vessel, the connector including an aperture through which the sheath can be deployed into the bioreactor vessel; and a collapsible bellows which houses the sheath when in an undeployed position, the bellows coupled to one end of the sheath, the bellows, the connector, and the sheath configured to form at least a portion of a hermetically sealable and sterilizable enclosure.05-26-2011
20110124034ENRICHMENT OF PROCESS FEEDSTOCK - The subject invention relates to novel methods for treating microbial biomass and uses thereof. In particular, this invention provides methods for production of lipids using ionic liquid solutions, and subsequent uses of biomass components in food, biofuels, and as chemical precursors. Further, this invention provides methods for recovering the ionic liquids using an antisolvent, thus enables subsequent reuse of the ionic liquids In addition, this invention provides practical methods for determining the lipid content of a biomass and screening potential lipid-producing microbial classes. This invention also provides a method of chemical dewatering the lipid-rich algae cells by ionic liquids with its subsequent reuse.05-26-2011
20110124033FLUORESCENCE BASED ASSAY TO DETECT SODIUM/CALCIUM EXCHANGER (NCX ) "REVERSE MODE" MODULATING COMPOUNDS - Transporters are an emerging target family with enormous potential, offering scientific and economic opportunities The sodium/calcium exchanger is an important mechanism for removing Ca05-26-2011
20110124032Methods and Compositions for Treating Carcinoma Stem Cells - Cancer stem cells (CSCs) have been prospectively isolated or identified from primary tumor samples, and shown to possess the unique properties of self-renewal and differentiation, and can form unique histological microdomains useful in cancer diagnosis. Such cancer stem cells are shown herein to have the phenotype of containing decreased levels of reactive oxygen species (ROS) relative to non-tumorigenic (non-stem cell) cancer cells, as well as expression of other protective pathways. The CSCs are further shown to be more resistant to ionizing radiation (IR) and certain chemotherapies and to express high levels of ROS genes.05-26-2011
20110124031Marker Detection for Characterizing the Risk of Cardiovascular Disease or Complications thereof - The present invention provides methods, systems, devices, and software for determining values for one or more markers in order to characterize a subject's risk of developing cardiovascular disease or experiencing a complication thereof (e.g., within the ensuing one to three years). In certain embodiments, the markers are those derived from a blood sample using a hematology analyzer operably linked to a software application that is configured to compute a risk score for a subject based on the values for the markers detected in the blood sample.05-26-2011
20110124030AUTOMATED LOADING MECHANISM FOR MICROBIAL DETECTION APPARATUS - The present invention is directed to a method and automated loading mechanism for loading an apparatus. The apparatus of the present invention may include a means for automated loading, a means for automated transfer and/or a means for automated unloading of a container (e.g., a specimen container). In one embodiment, the apparatus can be an automated detection apparatus for rapid non-invasive detection of a microbial agent in a test sample. The detection system also including a heated enclosure, a holding means or rack, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system.05-26-2011
20110124029AUTOMATED LOADING MECHANISM FOR MICROBIAL DETECTION APPARATUS - The present invention is directed to a method and automated loading mechanism for loading an apparatus. The apparatus of the present invention may include a means for automated loading, a means for automated transfer and/or a means for automated unloading of a container (e.g., a specimen container). In one embodiment, the apparatus can be an automated detection apparatus for rapid non-invasive detection of a microbial agent in a test sample. The detection system also including a heated enclosure, a holding means or rack, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system05-26-2011
20110124028AUTOMATED MICROBIAL DETECTION APPARATUS - A method and automated apparatus for rapid non-invasive detection of a microbial agent in a test sample is described herein. The apparatus may include one or more means for automated loading, automated transfer and/or automated unloading of a specimen container. The apparatus also includes a detection system for receiving a detection container, e.g., container or vial, containing a biological sample and culture media. The detection system may also include one or more heated sources, holding structures or racks, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent therein. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system.05-26-2011
20110124026MULTI-PURPOSE SUBSTRATES USEFUL FOR CELL CULTURE AND METHODS FOR MAKING AND USING THE SAME - Described herein are multi-purpose substrates composed of (1) a base coated with a calcium phosphate coating and (2) a fluorophore-labeled collagen adsorbed on the calcium phosphate coating. The multi-purpose substrates are useful in culturing and studying the activity of a variety of cells. The multi-purpose substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.05-26-2011
20110124025Cell Collecting Devices and Methods for Collecting Cells - A cell collecting device having a housing with an inlet for receiving cells, a cell attractant cavity have a cell attractant, a cell collection channel running from the inlet to the cell attractant cavity, and a plurality of electrodes positioned to detect the presence of cells is disclosed. The cell attractant cavity may include a porous medium, such as, a hydrogel, containing the cell attractant, such as, epidermal growth factor. The channel may include a plurality of restrictions and expansions to assist in maintaining the porous medium while permitting the passage of attractant and cells. The device may be implanted into a patient for an extended period of time, and then removed and examined. A method for collecting cells, an implantable attractant dispersing device, and a porous medium for controlled releasing of a compound are also disclosed.05-26-2011
20110039294SYSTEM AND METHOD FOR MONITORING CARDIOMYOCYTE BEATING, VIABILITY AND MORPHOLOGY AND FOR SCREENING FOR PHARMACOLOGICAL AGENTS WHICH MAY INDUCE CARDIOTOXICITY OR MODULATE CARDIOMYOCYTE FUNCTION - Devices and methods for performing extracellular recording of cells, such as excitable cells, cardiomyocytes, and cardiomyocyte precursor cells is provided. An exemplary device includes a nonconductive substrate forming or provided as a base of one or more wells; a recording electrode positioned on the substrate within the well, wherein the recording electrode is accessible to cells when a cell sample is added to the device; and a reference electrode positioned within the well in a cell-free zone, the cell-free zone characterized as free from contact with cells when the cell sample is added to the device, thereby preventing contact between cells and the reference electrode.02-17-2011
20100311102Electrophysiology Methods for Identification of Protein Structures in Membranes Associated to Neurodegenerative Diseases - This invention corresponds to an in vitro method that employs an electrophysiology technique; in particular, an embodiment of the patch-clamp technique in its perforated type, which will allow us evaluating the neurotoxic capacity of protein structures associated to the generation of neurodegenerative diseases. It addition, it allows evaluating potential pharmacologic capacities of candidate molecules (drugs) in order to prevent, treat, or cure the said diseases. The invention involves the use of peptides that cause neurodegenerative diseases in order to form a spontaneous perforated recording that only occurs with peptides in the patch clamp glass pipette.12-09-2010
20120231490Method Of Differentiation From Stem Cells To Hepatocytes - Disclosed are: a gene transduction method for use in the induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes effectively; stem cells into each of which a gene useful for the induction of the differentiation into hepatocytes is introduced; and hepatocytes produced from stem cells each having the gene introduced therein. A specific gene can be introduced into stem cells such as ES cells or iPS cells using an adenovirus vector. The effective induction of the differentiation into hepatocytes can be achieved by introducing the gene. Specifically, the effective induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes can be achieved by introducing at least one gene selected from HEX gene, HNF4A gene, HNF6 gene and SOX17 gene into the stem cells.09-13-2012
20080318266FLUORESCENCE POLARIZATION INSTRUMENTS AND METHODS FOR DETECTION OF EXPOSURE TO BIOLOGICAL MATERIALS BY FLUORESCENCE POLARIZATION IMMUNOASSAY OF SALIVA, ORAL OR BODILY FLUIDS - The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.12-25-2008
20100330610METHODS OF PROCESSING A BIOLOGICAL GROWTH PLATE IN A BIOLOGICAL GROWTH PLATE SCANNER - Methods are provided to process a biological growth plate using a biological growth plate scanner. The scanner includes an imaging device, a processor, and an image processing profile memory. The scanner scans a plate type indicator associated with the biological growth plate. In some embodiments, the processor uses the plate type indicator to verify the suitability of the biological growth plate for use in the scanner. In some embodiments, the processor uses the plate type indicator to unlock the scanner for operation.12-30-2010
20080299599FLUORESCENT PROTEINS FOR MONITORING INTRACELLULAR SUPEROXIDE PRODUCTION - Protein probes and methods for measuring real-time changes in intracellular superoxide formation are provided. The probes include superoxide sensitive variants of yellow fluorescent and green fluorescent proteins. The probes, or nucleic acids encoding the probes, may be delivered to cells or organisms. Changes in the fluorescence of the probes may then be detected using standard real-time fluoroscopy techniques.12-04-2008
20080299603Predictive assay for immune response - The present invention relates to an in vitro method for determining the ability of a vaccine composition which comprises one or more antigens or a nucleic acid molecule which encodes one or more antigens to stimulate a T cell response. In one embodiment, the method comprises the steps of: (1) contacting antigen presenting cells in culture with a vaccine composition selected from among the group of vaccine compositions, thereby, if one or more of the antigens or nucleic acid molecules can be taken up and processed by the antigen presenting cells, producing one or more processed antigens; (2) contacting the antigen presenting cells with T cells under conditions sufficient for the T cells to respond to one or more of the processed antigens; (3) determining whether the T cells respond to one or more of the processed antigens; whereby if the T cells respond to one or more of the processed antigens, then the vaccine composition stimulates a T cell response; and (4) repeating steps (1), (2) and (3) with each vaccine composition in the group, thereby identifying vaccine compositions which stimulate a T cell response; and, if one or more of the vaccine compositions stimulates a T cell response, (5) selecting at least one vaccine composition which stimulates a T cell response for assessment in one or more animals and/or human subjects.12-04-2008
20080299602Methods of Employing Vascular Leakage to Diagnose a Capillary Leak Disorder - A method of diagnosing a capillary leak disorder in a patient includes monitoring the marinobufagenin level in blood and/or urine as indicators of vascular permeability and, if a substantial elevation in marinobufagenin exists with respect to that of a normal person, concluding that a capillary leak disorder exists. The method may be employed to diagnose preeclampsia, as well as illnesses or abnormal conditions selected from the group consisting of acute respiratory distress syndrome, hemorrhagic shock, septic, endotoxemia, septicemia, burns.12-04-2008
20080299600Apparatus Assembly and Method for Detecting an Analyte - An apparatus assembly for detecting an analyte in a sample of material includes a generally self-contained housing and an indicator cap. The housing is moveable between a sample preparation orientation and a testing orientation and is configured to interchangeably receive a sample collection device and the indicator cap having a testing device for detecting the analyte.12-04-2008
20080299598Detector Comprising a Membrane Perturbation - Detecting Polymer and Functional Membrane Fragments - A construct comprising functional membrane fragments and one or more perturbation-detecting polymers associated therewith, wherein said construct responds to perturbations of said membrane fragments by means of a detectable change in one or more physical or chemical properties associated with said construct.12-04-2008
20110086380DEVICE FOR STORING A BIOLOGICAL SAMPLE AND FOR PREPARING THE BIOLOGICAL SAMPLE - A device for storing a biological sample (04-14-2011
20110003327METHODS FOR PRODUCTION OF ATRIAL PROGENITORS AND THEIR DIFFERENTIATION INTO SMOOTH MUSCLE CELLS AND CARDIOMYOCYTES - The present invention generally relates to methods to identify and isolate atrial progenitors, and in some embodiments to the atrial progenitors are positive for both Islet 1 (Isl1) and sarcolipin (SLN). One aspect of the present invention relates to methods to differentiate progenitors into Isl1+/SLN+ atrial progenitors. Another aspect of the invention relates to methods to differentiate Isl101-06-2011
20110003325MICROFLUIDIC DEVICE - The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard sterilization capabilities. In other embodiments, microfluidic devices have integral collection bags and methods for keeping the microfluidic channels clean.01-06-2011
20110003324MICROFLUIDIC DEVICE HAVING ONBOARD TISSUE OR CELL SAMPLE HANDLING CAPABILITY - The present disclosure is generally directed to systems for the storage and preservation of an original tissue or cell sample onboard a microfluidic device, such as a cytometry chip. In some embodiments, the sample may be disassociated while onboard the microfluidic device.01-06-2011
20110003323Bioreactor systems and associated methods of processing bioreactor vessels - A bioreactor processing unit (01-06-2011
20090286277PROGNOSTIC METHOD - In one aspect a method of determining the functional activity of the MRP2 and/or MRP3 efflux pathway of a human or animal subject, comprises: 11-19-2009
20110039293Effervescent Solid Pharmaceutical Composition Comprising Dextrose and Process for its Preparation - The present invention is related to the medicine field. Specifically, the present Invention is related with an effervescent solid pharmaceutical composition of dextrose having as purpose determining the metabolized glucose levels in a patient with diabetes or glucose metabolism related conditions, in a simple and effective manner with respect to the conventional products of this kind. Accordingly, the present invention provides a novel product allowing the medical professional or physician of a clinic laboratory to determine in a quantitative, economic, efficient, fast and in a single manner, the clinical conditions of a patient regarding the glucose level and the pancreas ability to eliminate the unnecessary sugars in terms of time. Furthermore, the present invention provides a novel process for preparing the novel effervescent solid pharmaceutical formulation of dextrose.02-17-2011
20120322096ANIMAL STROKE MODEL - An animal stroke model is provided. The model is useful in the study of brain ischemia and/or reperfusron injury and in identification and testing of compounds and interventions useful in the treatment of stroke. The method is carried out in a non-human mammal, such as rat. The method of inducing ischemia and/or reperfusion injury involves exposing a portion of the middle cerebral artery (MCA) and temporarily occluding it at one or more distinct locations, preferably three distinct locations. The model results in highly reproducible and focal infarct sizes with low rate of mortality during the experimental procedure.12-20-2012
20110045524TRANSFECTION READY EUKARYOTIC CELLS - Described herein are frozen populations of transfection ready competent eukaryotic cells, transfection kits comprising the frozen populations of transfection ready competent cells, and methods of using the same.02-24-2011
20110045522METHODS FOR DIAGNOSING DIABETES AND DETERMINING EFFECTIVENESS OF TREATMENTS - The invention generally relates to novel methods of measuring the effectiveness of a drug for the treatment of diabetes and methods of diagnosing diabetes. In some embodiments of the invention, IL-1Ra is used as a biomarker to measure the effectiveness of a drug for the treatment of diabetes.02-24-2011
20110045521SAMPLE PROCESSING APPARATUS AND SAMPLE PROCESSING METHOD - According to an embodiment, a sample processing apparatus includes, a processing unit configured to subject a sample container or a sample contained in the sample container to processing, and a label removal unit configured to remove at least part of a label affixed to a side part of the sample container prior to the processing.02-24-2011
20110045520FATTY ACID MARKERS FOR THE DIAGNOSIS, PROGNOSIS AND MANAGEMENT OF CARDIOVASCULAR DISEASE - Methods of detecting myocardial infarction are disclosed based on elevated levels of one or more free fatty acids. The methods may comprise detection of elevated levels of total free fatty acids in a sample relative to average total free fatty acid levels in a control subject without myocardial infarction. Also disclosed are methods to detect myocardial infarction comprising detection of elevated levels of individual free fatty acids in a sample relative to those levels in a control subject and methods comprising determining whether the molar ratio of total free fatty acids to HSA is indicative of myocardial infarction.02-24-2011
20110045519DIFFERENTIATED IMMORTALISED CELL LINES CAPABLE OF PRODUCING ALBUMIN AND BLOOD COAGULATION FACTORS, METHODS OF PREPARING THEREOF FROM A LEUKAEMIA CELL LINE AND USES THEREOF - The invention relates to cell lines from differentiated cells with hepatocytic phenotypes capable of producing albumin and blood coagulation factors, said cells being derived from a human leukaemia cell line, preferably the human THP1 cell line, and preserving the characteristics of immortality. Among the cell lines of the invention, the cell lines known as PSC-THP1-EP, PSC-THP1-EP-FAST, PSC-THP1-HEP and PSC-THP1-EPEP are preferred. The invention also relates to methods for obtaining the cell lines of the invention and the uses of said cell lines, particularly for the production of albumin and/or blood coagulation factors.02-24-2011
20120270256Two-Photon Endoscopic Scanning Assembly for Inflammatory Disease Detection - An endscopic imaging device is described that achieves longitudinal axis (z-axis) scanning into a tissue or sample, using a piezoelectric microactuator. In some configurations, additional lateral (xy-plane) scanning is also achieved, to allow for the creation of full three-dimensional imaging, ex vivo or in vivo. The techniques may be used to image and diagnosis allergic rhinitis and eosinophilic esophagitis in tissue.10-25-2012
20120270257Observation Cell Arrangement - An observation cell arrangement for flow perfusion of a sample to be examined, the arrangement comprising a flow cell (10-25-2012
20090130701NOVEL SEQUENCE VARIANTS OF MULTI-DRUG RESISTANCE GENES, MDR1 AND MRP1, AND RECOMBINANT CELLS EXPRESSING MRP1 AND MDR1 FOR ASSESSMENT OF DRUG PENETRATION AND DISPOSITION - Provided are compositions relating to novel MDR1 polymorphisms, including nucleic acids, polypeptides, and recombinant cells, as well as methods for detection of MDR1 polymorphisms in biological samples and elucidation of the influence of MDR1 polymorphisms on MDR1 protein function. Also provided are a rat MRP1 cDNA and protein, stable cell lines expressing the rat MRP1 protein, and methods of assessing drug penetration or disposition in a cell line expressing a recombinant mammalian MRP1 or MDR1 protein, or a homolog thereof.05-21-2009
20110212479MEASUREMENT OF SEQUESTERED CARBON - Disclosed herein are methods and systems for determining an amount of carbon dioxide that can he sequestered from the atmosphere by a portion of an ocean. The methods or systems involve the following steps (a) determining which chemical element or elements would, when added to a photic zone of the portion, cause growth of a phytoplankton population in the portion; (b) determining a quantity of the chemical element or elements to be added to the photic zone whereby substantially all of the element or substantially all of at least one of the elements added would be consumed by the additional phytoplankton grown: and (c) calculating the amount of carbon dioxide that can be sequestered from the atmosphere based on the determined quantity of the chemical element or elements that would be added to the portion.09-01-2011
20110229925ASSAYS FOR PERFORMANCE OF ORGANISMS IN PHENOTRONS - A phenotron assay can be used for determining an adverse effect on health by: identifying an exposure condition to be studied for an adverse effect on the health of an organism; providing a phenotron having one or more sensors each configured to sense a signal; introducing one or more test founder organisms into the phenotron, said test founder organisms each having a signal emitting tag that is sensed by the one or more sensors and each being exposed to the exposure condition; introducing one or more control founder organisms into the phenotron, said control founder organisms each having a signal emitting tag that is sensed by the one or more sensors and none of said control founder organisms being exposed to the exposure condition; collecting, from the one or more sensors, signals from the tags to obtain data indicative of a parameter of the health of the one or more test founder organisms compared to the one or more control founder organisms; and determining from the data whether the exposure condition has an adverse effect on the health of the one or more test founder organisms.09-22-2011
20100203580Methods and Apparatus for the Location and Concentration of Polar Analytes Using an Alternating Electric Field - A method is disclosed for effecting the concentration of a polar analyte in an alternating electric field. In the method, a relative translation of the polar analyte and an alternating electric field along a translation path is effected. A portion of the polar analyte is then trapped and concentrated in a concentration zone formed by the intersection of the translation path and the alternating electric field. Also disclosed are various devices for carrying out the forgoing method.08-12-2010
20100203579METHOD OF MEASURING AND COMPARING LEVELS OF SUBSTANCES IN AN INDIVIDUAL'S BODY - A method for measuring the homeostatic relationship between various substances in the body. The measured levels of substances in the body use the interrelationships of the various substances in order to establish guidelines for treating individuals.08-12-2010
20100203574METHOD AND APPARATUS FOR CULTIVATING LIVING CELLS - The invention relates to a method for cultivating living cells 08-12-2010
20100203577METHODS OF USING GPR119 TO IDENTIFY COMPOUNDS USEFUL FOR INCREASING BONE MASS IN AN INDIVIDUAL - The present invention relates to methods of using GPR119 receptor to identify compounds useful for increasing bone mass in an individual. Agonists of GPR119 receptor are useful as therapeutic agents for treating or preventing a condition characterized by low bone mass, such as osteoporosis, and for increasing bone mass in an individual. Agonists of GPR119 receptor promote bone formation in an individual.08-12-2010
20100203576PROCESS FOR DETERMINATION OF MICROORGANISMS' RESISTANCE TO ANTIBIOTICS - A process for determination of microorganisms' projected resistance to antibiotics that includes analyzing a first microorganism by mass spectrometry so as to obtain a first mass spectrum of the first microorganism, whereby the process is characterized in that an analytical device carries out the following stages: 08-12-2010
20100203573AUTOMATED INSTRUMENTATION AND METHOD FOR MEASUREMENTS OF SAMPLES - One object of the invention is to provide instrumentation and a method for efficient and reliable assaying and measuring samples. The teachings include an automated self-contained instrument for assaying and measuring samples, wherein the samples are located on wells of sample plates, and the instrument comprises a plurality of units for processing or storing sample plates. The instrument according to the teaching may comprise at least one dispensing unit for dispensing reagents or other assay components to the sample wells, at least two units for simultaneously processing or storing a plurality of sample plates, at least one unit for removing substance from the sample wells, and one or several measurement units, the measurement unit(s) providing a capability for the instrument to optically measure samples in at least two measurement modes. Further, the instrument comprises a manipulator having a capability to move the sample plates in three orthogonal directions or combinations thereof and to rotate the sample plates in relation to a vertical axis for transferring the sample plates to the units.08-12-2010
20100203572METHOD FOR CARRYING OUT AND EVALUATING MIX & MEASURE ASSAYS FOR THE MEASUREMENT OF REACTION KINETICS, CONCENTRATIONS AND AFFINITIES OF ANALYTES IN MULTIPLEX FORMAT - The invention relates to a method comprising the following steps: a) use of a support which has at least two different microparticle populations immobilized thereon; b) measuring the fluorescence of the support from step a) with an optical resolution 1, said resolution 1 permitting differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers and determination of the localized position of individual immobilized microparticles; c) contacting the support from step a) with the sample to be analyzed; d) performing at least one additional measurement of the fluorescence of the support during or after contacting in accordance with step c) with a resolution 2; e) assigning the fluorescence values measured with resolution 2 to the individual microparticle singlets, doublets, triplets, multiplets and monolayers locally identified on the support in accordance with step b) and assigned to a particular acceptor molecule population; f) determining the change in fluorescence. The method is used to determine reaction kinetics, concentrations and affinities of analytes in samples.08-12-2010
20100203571BIOCHEMICAL MARKERS FOR ACUTE PULMONARY EMBOLISM - The present invention relates to a method of differentiating between a singular and a multiple lung embolism in a subject suspected to suffer from acute lung embolism comprising determining the amount of NT-proBNP in a sample of a subject suspected to suffer from acute lung embolism and comparing the amount to a reference amount. Further, the present invention also relates to a method of differentiating between acute and chronic lung embolism in a subject comprising determining the amount of NT-proANP at a first and a second time point and comparing the determined amounts with each other. The present invention also encompasses devices and kits for carrying out the aforementioned methods.08-12-2010
20100203570GENE SPECIFICALLY EXPRESSED IN POSTMITOTIC DOPAMINERGIC NEURON PRECURSOR CELLS - A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.08-12-2010
20100203569Method for Evaluating Risk in Multiple Sclerosis - The invention relates to methods and reagents for diagnosing and assessing the prognosis of multiple sclerosis. The present invention is based, in part, upon the discovery that anti-glycan antibodies are useful in evaluating the risk of whether clinically isolated syndrome (CIS) patients suggestive of Multiple sclerosis (MS) will have a clinical relapse within, e.g., 24 months. The invention is also based upon the discovery that anti-glycan antibodies are useful for evaluating the risk of CIS patients suggestive of MS to have a rapid disease progression and accumulate disabilities, e.g., permanent disability, within a certain time frame, e.g., 5 years.08-12-2010
20100203568Assay Method For Peptide Specific T-Cells - A method of assaying for peptide-specific T-cells comprises adding peptide to a fluid sample of fresh peripheral blood mononuclear cells, and detecting a cytokine such as interferon-γ produced by T-cells that have been pre-sensitised to the peptide. The assay method is quick and cheap and is expected to be useful for the study of various disease states including Hepatitis B, Hepatitis C, tuberculosis, malaria, HIV and influenza.08-12-2010
20110236921CELL SYSTEM - The present invention relates to cellular systems for testing drug candidates and for evaluating the function of mitochondrial proteins. The invention is particularly useful for evaluating drug candidates for cancer and for conducting studies on drug resistance.09-29-2011
20120129208HONEYCOMB SHRINK WELLS FOR STEM CELL CULTURE - This invention provides a microwell array having a plurality of microwells on a hydrophobic surface wherein the microwells each is substantially proximate to each of its adjacent microwells, as well as methods to prepare arrays. Also provided is a plate that comprises at least one microarray, at least one input channel, at least one output channel, and a channel connecting the input and output channel.05-24-2012
20120276573NANOTUBE STRUCTURES, METHODS OF MAKING NANOTUBE STRUCTURES, AND METHODS OF ACCESSING INTRACELLULAR SPACE - In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.11-01-2012
20100233755PDX1-EXPRESSING DORSAL AND VENTRAL FOREGUT ENDODERM - Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1-positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1-positive foregut endoderm cells, are also disclosed.09-16-2010
20100233754Vessel Transporting Apparatus and Method - This disclosure relates to an apparatus for transporting a vessel and to a sorting apparatus. The apparatus has at least one support device adapted to support the vessel in a reclined orientation, and a pushing mechanism adapted to transport the vessel along the support device. Advantageously, the apparatus is relatively simple and does not require vessels to contact an endless conveyor. Thus, vessels can be more readily rotated, weighed, etc. This disclosure also relates to a method of sorting vessels. One application of the apparatus and method is in the sorting of test tubes containing blood samples for testing.09-16-2010
20100233753OPTICAL MEASURING DEVICE AND METHOD THEREFOR - The light measurement apparatus of the invention, in which the light is irradiated to the sample dispersed in the liquid flowing through the flow passage, is used for measuring optical information of the sample. The apparatus includes a light source portion 09-16-2010
20100233752METHOD FOR DIAGNOSIS AND MONITORING OF DISEASE ACTIVITY AND RESPONSE TO TREATMENT IN SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) AND OTHER AUTOIMMUNE DISEASES - The present invention provides methods of diagnosing and monitoring systemic lupus erythematosus and drug-induced lupus erythematosus by measuring cell-based complement activation products in a subject's blood. In particular, the invention describes a diagnostic method employing the measurement of multiple complement activation products, such as C309-16-2010
20100233751APPARATUS AND METHOD FOR MONITORING CULTURES - Disclosed is a bubble excluder device (09-16-2010
20100233749Device with biological component and method of making to achieve a desired figure of merit - An improved method for the design and development of high performance hybrid devices having biologically-derived and nonbiological components and the hybrid devices so-designed and developed. A desired figure of merit is determined for the biologically-derived component or components. The organism from which the biologically-derived component is derived is subjected to various environmental variables as it is grown. Organisms providing biologically-derived components having the desired figure of merit are identified. The biologically-derived component is thereafter developed from organisms force adapted to cause the biologically-derived component figure of merit to reach a goal or an acceptable measure. The biological component is used in hybrid constructs that may be nanostructures, given the small size of the biological parts. In one specific embodiment, force-adapted chlorosomes of 09-16-2010
20120276574MICROFLUIDIC MIXER - Provided is a microfluidic or nanofluidic apparatus which comprises:11-01-2012
20120276575Unit and Device for the Preparation of Cells and/or Particles in a Liquid and Method for Microscopic Analysis11-01-2012
20120276576POROUS POLYMER MONOLITHS, PROCESSES FOR PREPARATION AND USE THEREOF - The present invention generally relates to porous polymer monoliths. The present invention also relates to processes for the preparation of porous polymer monoliths, storage mediums formed from porous polymer monoliths and use thereof in the drying and storage of body fluids including blood and blood plasma samples.11-01-2012
20080311607Methods and Compositions for Regulation of Stem Cell Survival, Proliferation, and Differentiation by Protein Ubiquitination - Compositions and methods for regulating in vitro cell growth are disclosed, and for providing undifferentiated stem cells or embryonic cells that are suitable for transplantation into damaged tissues or organs, or for use in tissue repair. A representative method includes causing the overexpression or underexpression of GalT binding protein (GtBP), also referred to as GalT associated protein (GTAP), in a cell such that ubiquitination of at least one cellular protein associated with cell adhesion and/or cell-to-cell interaction is correspondingly increased or decreased, causing inhibition of cell growth when GTAP is overexpressed and causing enhanced cell growth when GTAP is underexpressed by the cell. As a result, growth of the cell is altered or regulated.12-18-2008
20110262958DEVICE FOR EXAMINING MYOCARDIAL TOXICITY, CHIP FOR EXAMINING MYOCARDIAL TOXICITY AND METHOD FOR EXAMINING MYOCARDIAL TOXICITY - [Problem] To provide a device and a method for examining myocardial toxicity, which can be realized in vitro in an equivalent manner as those conventionally carried out in vivo.10-27-2011
20110262951COLLECTION DEVICE AND MATERIAL - Swabs, and materials of the present disclosure, and methods of making same, include randomly arranged sea-island bicomponent fibers.10-27-2011
20120088263Sample Test Cards - The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprise one or more fluid over-flow reservoirs, wherein the over-flow reservoirs are operatively connected to a distribution channel by a fluid over-flow channel. In another embodiment, the sample test cards may comprise a plurality of flow reservoirs operable to trap air thereby reducing and/or preventing well-to-well contamination. The test card of this invention may comprise from 80 to 140 individual sample wells, for example, in a test card sample test cards of the present invention have a generally rectangular shape sample test card having dimensions of from about 90 to about 95 mm in width, from about 55 to about 60 mm in height and from about 4 to about 5 mm in thickness.04-12-2012
20120088262CYANINE COMPOUNDS, CONJUGATES AND METHOD OF USE - Cyanine compounds having the general formula I, conjugates, complexes, and compositions comprising the cyanine compounds are provided. Fluorescence resonance energy transfer (FRET) dye pairs and viability dyes are also provided.04-12-2012
20120088261CELL LABELING AND IMAGING USING MULTIFUNCTIONAL PERFLUOROCARBON NANOEMULSION - The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In particular, the perfluorocarbon nanoemulsion containing optical nanoparticle is provided with both optical characteristics and 04-12-2012
20090142794Mutated anthrax toxin protective antigen proteins that specifically target cells containing high amounts of cell-surface metalloproteinases or plasminogen activator receptors - The present invention provides methods of specifically targeting compounds to cells overexpressing matrix metalloproteinases, plasminogen activators, or plasminogen activator receptors, by administering a compound and a mutant protective antigen protein comprising a matrix metalloproteinase or a plasminogen activator-recognized cleavage site in place of the native protective antigen furin-recognized cleavage site, wherein the mutant protective antigen is cleaved by a matrix metalloproteinase or a plasminogen activator overexpressed by the cell, thereby translocating into the cell a compound comprising a lethal factor polypeptide comprising a protective antigen binding site.06-04-2009
20100216180METHODS OF USING HALOGENATED PEPTIDES AS INTERNAL STANDARDS FOR LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY - Methods of using halogenated peptides as internal standards for liquid chromatography-mass spectrometry, and novel halogenated peptides useful for the same, are disclosed. In particular, methods of using halogenated peptides as internal standards in proteomic analyses, as well as methods of using halogenated peptides to conduct quality control assessments of and/or to calibrate liquid chromatography-mass spectrometry systems are disclosed.08-26-2010
20120329084HIGH THROUGHPUT SCREENING OF LACTIC ACID-PRODUCING MICROORGANISM - A high throughput screening system and method of a lactic acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of a lactic acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.12-27-2012
20120288886INHIBITING BINDING OF FGF23 TO THE BINARY FGFR-KLOTHO COMPLEX FOR THE TREATMENT OF HYPOPHOSPHATEMIA - The present invention is directed to a method of treating hypophosphatemia in a subject. The present invention is also directed to a method of screening for compounds suitable for treatment of hypophosphatemia associated with elevated or normal FGF23. This method involves providing FGF23, FGFR-Klotho complex, and one or more candidate compounds. The FGF23, the FGFR-Klotho complex, and the candidate compounds are combined under conditions effective for the FGF23 and the binary FGFR-Klotho complex to form a ternary complex if present by themselves. This method also involves identifying the candidate compounds, which prevent formation of the complex as being potentially suitable in treating hypophosphatemic conditions associated with elevated or normal FGF23. A method of screening the specificity of compounds which prevent formation of the FGF23-Klotho-FGFR complex is also disclosed.11-15-2012
20120288887BLOOD CELL AGGLUTINATION IMAGE DETERMINING METHOD AND BLOOD CELL AGGLUTINATION IMAGE DETERMINING APPARATUS - The present invention provides a blood cell agglutination image determining method and a blood cell agglutination image determining apparatus capable of processing a blood sample in a short time and obtaining a reproducible determination result. Provided are a blood cell agglutination image determining method and a blood cell agglutination image determining apparatus for determining a blood sample to be positive or negative based on a blood cell agglutination image of a reaction between a blood sample and a reagent in a reaction container. The apparatus comprises: a rotation mechanism R for rotating a reaction container so that a bottom wall of the reaction container will turn outwards by centrifugal force; and an inclining apparatus 11-15-2012
20120100570Site-Specific Incorporation of Redox Active Amino Acids into Proteins - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.04-26-2012
20120100569SUPERCHARGED PROTEINS FOR CELL PENETRATION - Compositions, preparations, systems, and related methods for delivering a supercharged protein, or a complex of a supercharged protein and an agent (e.g., nucleic acids, peptides, proteins, small molecules) to cells are provided. Such systems and methods include the use of supercharged proteins. For example, superpositively charged proteins may be associated with nucleic acids (which typically have a net negative charge) via electrostatic interactions. In some embodiments, such systems and methods involve altering the primary sequence of a protein in order to “supercharge” the protein (e.g., to generate a superpositively-charged protein). In some embodiments, complexes comprising supercharged proteins and one or more agents to be delivered are useful as therapeutic agents. In some embodiments, complexes and/or pharmaceutical compositions thereof are administered to a subject in need thereof. The inventive complexes or pharmaceutical compositions thereof may be used to treat proliferative diseases, infectious diseases, cardiovascular diseases, inborn errors in metabolism, genetic diseases, etc.04-26-2012
20120100568SERUM-FREE MEDIUM FOR INDUCING PLURIPOTENT STEM CELLS QUICKLY WITH HIGH EFFICIENCY AND METHOD USING THEREOF - A serum-free medium for inducing and reprogramming somatic cells into induced pluripotent stem cells (iPS) quickly with high efficiency, and the method using thereof for inducing and reprogramming somatic cells without feeder are provided, wherein the rate and efficiency of whole process of inducing and reprogramming are greatly improved. The uses of the medium in inducing pluripotent stem cells, and the uses in the method for screening compounds, especially in the method for high throughput screening compounds are further provided.04-26-2012
20100055733MANUFACTURE AND USES OF REACTIVE MICROCONTACT PRINTING OF BIOMOLECULES ON SOFT HYDROGELS - Embodiments of the present disclosure encompass microfabrication methods (“reactive microcontact printing of soft matter”) for hydrated soft polymer materials and surfaces for culture platforms suitable for the culturing of isolated single primary mammalian cells in an environment approximating the natural niches of the cells. Such culture platforms may comprise arrays of microwells, or other microscopically textured features, in which individual features can comprise desired proteins or mixtures of proteins. The microfabrication methods of the disclosure allow spatial control of surface biochemistry and topography at the micrometer scale on these hydrated soft gels. The hydrogels and methods of manufacture and use of the disclosure allow the isolation of a single stem cell and the characterizing of its interaction with cytokines and morphogens, especially with regard to modulation of the proliferative capacity of the stem cell when implanted in a recipient host. The systems for isolating or culturing a eukaryotic cell comprise a hydrogel film comprising a cross-linked polymeric composition having the characteristic of hydrating to form a hydrogel and having a topographical feature or a plurality of topographical features that may have a surface capable of receiving and immobilizing at least one biomolecule species thereon.03-04-2010
20100167337DEVICE FOR CELL SEPARATION AND ANALYSIS AND METHOD OF USING - A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.07-01-2010
20100167334SYSTEMS AND METHODS FOR PROCESSING TISSUE SAMPLES FOR HISTOPATHOLOGY - A system for processing tissue biopsy samples during a histopathology process, including a tissue carrier constructed to carry a tissue biopsy sample during at least one step of the histopathology process, a database storing information associated with the tissue biopsy sample and associated with a tissue processing procedure, a machine-readable indicator physically associated with the tissue carrier, the machine-readable indicator including a machine-readable reference identified with the tissue biopsy sample, and an electronic control operative to read the reference, access the information in the database using the reference, and implement at least a portion of the tissue processing procedure in accordance with the accessed information.07-01-2010
20100167329DEVICE FOR DRUG SCREENING - The present invention discloses a device and an optical liquid sensor for drug screening, and a method for using these. More particularly a device, an optical liquid sensor, and a method, for repeatable measurements of the effect of a test drug on a biological tissue sample are described.07-01-2010
20100167336PARAMETER FOR X- AND Y- CHROMOSOME BEARING SPERM SORTING WITH HIGH DEGREE OF PURITY - Current invention is about the sex-specific separation of sperm in high purity. For more details, this invention is about the methods for improving the separation efficiency of sperm by removing factors deteriorating separation efficiency by utilizing several parameters and adjusting few conditions for the purpose of separating sperm into X-chromosome bearing and Y-chromosome bearing groups in high purity. Current invention, for the purpose of separating X-chromosome bearing and Y-chromosome bearing sperm based on physiological characteristics, utilize the difference of sperm's nucleus width as a parameter to recognize the difference in DNA content between X and Y chromosome. Current invention is about the sex-specific separation of sperm in high purity. This invention, unlike any known conventional methods, requires neither special conditions nor special treatments, can be conducted in a more realistic environment and, as a results, sex-specific sorting of sperm can be conducted without losing viability or motility. Therefore, the limitation conferred on sorting efficiency by the motility can be overcome by our invention.07-01-2010
20100167335METHODS FOR DIAGNOSING AND TREATING ALLERGIES - The present invention relates to methods of screening actives for the treatment of allergic reactions and providing treatment therefor. In particular, the invention relates to the screening of various antibacterial actives for treatment of asthma and other related symptoms.07-01-2010
20100167330MICROMECHANICAL DEVICES FOR CONTROL OF CELL-CELL INTERACTION, AND METHODS OF USE THEREOF - The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.07-01-2010
20100167333Heavy Metal Binding Compounds and Their Method of Use - The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula07-01-2010
20100068752Novel fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest - The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties.03-18-2010
20100129852Integrated Bioanalyzer - The present invention relates to an in vitro assay method and device that provides for detection and measurement of entities in a fluid sample that can be captured and concentrated in a unitized self-contained enclosed filter apparatus that is analyzed in an optical detection instrument for indications of the entity. It provides for analysis of biological material including cells, their enzymes, or other constituents thereof, that can be identified based on an indicator-generating means. The analyses provided for include detection of the presence of the entity, and changes in the entity over time, such as associated with growth and increasing metabolic activity with an expanding population of cells, or decreasing metabolic activity, for example, due to presence of inhibitory or toxic agents.05-27-2010
20100129854LIVER CELL TOXICITY ASSAY - The disclosure provides methods for characterizing the toxicity of a candidate molecule to liver cells as defined herein; methods of culturing metabolically active liver cells on a biosensor as defined herein; and biosensor liver culture systems as defined herein.05-27-2010
20100129856Methods and Devices for Cell Signaling Under Pulse Stimulation - The disclosure provides a device for measuring cellular responses under controlled environments. The disclosure also provides an array of devices for measuring cellular responses under controlled environments. The disclosure also provides methods to study cell signaling using the devices. By using microfluidics, the disclosure enables study of cell signaling under well-defined environment conditions. Such capability can be used for differentiating long-acting ligands from short-acting ligands, for determining the kinetics of receptor resensitization and functional recovery of receptor signaling, and for differentiating the sensitivity of a cell type to laminar flow-induced stress force. Through combination with conventional static label-free cell assays, the full spectrum of a drug compound can be studied in details, thus creating a complete representation of its pharmacology acting on living cells.05-27-2010
20100129851METHODS OF DIAGNOSING MUSCLE DAMAGE - A method for assessing muscle damage in a biological sample obtained from a subject is disclosed. The method involves obtaining a biological sample from a subject being assessed for muscle damage, and evaluating the sample for the presence or absence of a myofilament protein modification product. Preferably, the myofilament protein modification product is a chemical adduct of a myofilament protein. The method can also be used to assess the extent and/or type of muscle damage in a subject by studying the profile of myofilament protein modification products detected in the sample taken from the subject. The invention further provides a method for screening for an agent which modulates the level of a myofilament protein modification product present in a biological sample or for a calcium sensitizing agent. The invention is applicable to cardiac muscle and skeletal muscle.05-27-2010
20100129853DISEASE MARKERS - The present invention relates to biomarkers for autoimmune disease, and in particular to a method for determining a status of an autoimmune disease in a test subject, comprising measuring production of IL-5 and IL-13, as well as to associated uses and kits.05-27-2010
20100129850METHOD FOR ANALYZING THE EFFECT OF A GASEOUS MEDIUM ON A BIOLOGICAL TEST SYSTEM USING AN EXTRACELLULAR METABOLIZATION SYSTEM AND A DEVICE FOR CARRYING OUT THE METHOD - The invention relates to a method for analyzing the effect of a gaseous medium on a biological test system using an extracellular metabolization system. The method consists of the following steps: a biological test sample is cultivated on a permeable carrier, the gaseous medium is guided over the surface of the biological test system in order to form an exposition atmosphere over the biological test system, the extracellular metabolization system is added to a conservation medium and the permeable carrier is brought into contact with a conservation medium that comprises the extracellular metabolization system below the permeable carrier, in such a manner that the extracellular metabolization system only passes through the permeable carrier and that the biological test system is not submerged by the conservation medium containing the extracellular metabolization system.05-27-2010
20100124762FLUIDIZED BED DETECTOR FOR CONTINUOUS, ULTRA-SENSITIVE DETECTION OF BIOLOGICAL AND CHEMICAL MATERIALS - The present invention is generally directed to a fluidized bed detector for continuous detection of biological and chemical materials comprising a fluidized bed of detecting elements suspended in a continuous flow system wherein the detecting elements remain in the system when a first force trying to move the detecting elements to the bottom of the system is balanced with a second opposing force of a flowing gas or liquid trying to move detecting elements to the top of the system and wherein the presence of a target molecule in the flowing gas or liquid disrupts the balance of the first and second forces causing the detecting element to exit the system. The release of the detecting element indicates the presence of the target molecule and may be captured, concentrated, or both for further evaluation by other assays or other means. Also disclosed is the related method of detecting biological and chemical materials using a fluidized bed detector.05-20-2010
20130017567ZINC OXIDE-BASED NANOSTRUCTURE MODIFIED QCM FOR DYNAMIC MONITORING OF CELL ADHESION AND PROLIFERATION - A dynamic and noninvasive method of monitoring the adhesion and proliferation of biological cells through multimode operation (acoustic and optical) using a ZnO nanostructure-modified quartz crystal microbalance (ZnO01-17-2013
20130017566METHOD FOR ASSESSING THE CONDITION OF SKIN AND/OR SCALPAANM KOSAGA; MasaruAACI SingaporeAACO SGAAGP KOSAGA; Masaru Singapore SGAANM HE; ShanAACI BeijingAACO CNAAGP HE; Shan Beijing CNAANM GONG; TianGuiAACI GuangzhouAACO CNAAGP GONG; TianGui Guangzhou CN - Method for assessing condition of skin and/or scalp by resulted color from the interaction of protein detecting composition with skin and/or scalp sample. Also disclosed is a method for comparing condition of different skin and/or scalp using the above method. Such conditions include, for example, skin healthiness and dandruff condition of scalp.01-17-2013
20110159533BIOPARTICLE CAPTURE DEVICE, AND USE THEREOF - A device for capturing suspended bioparticles in a liquid medium, includes: 06-30-2011
20110159532GEF-H1b: BIOMARKERS, COMPLEXES, ASSAYS AND THERAPEUTIC USES THEREOF - The present invention relates to diagnosing abnormal cell proliferation in biological samples and screening for drugs which inhibit, reduce or abolish cell growth, especially tumorigenic cell growth, by detecting a phosphovariant isoform of a guanine nucleotide exchange factor biomarker, such as the novel GEF-H1S.06-30-2011
20080254499Multiphoton in Vivo Flow Cytometry Method and Device - The invention relates to a method for diagnosing a disease state mediated by pathogenic cells, said method comprising the steps of administering to a patient a composition comprising a conjugate or complex of the general formula10-16-2008
20080254497Response Predictors for Erbb Pathway-Specific Drugs - The invention provides a method of determining whether tumor cells or tissue is responsive to treatment with an ErbB pathway-specific drug. In accordance with the invention, measurements are made on such cells or tissues to determine values for total ErbB receptors of one or more types, ErbB receptor dimers of one or more types and their phosphorylation states, and/or one or more ErbB signaling pathway effector proteins and their phosphorylation states. These quantities, or a response index based on them, are positively or negatively correlated with cell or tissue responsiveness to treatment with an ErbB pathway-specific drug. In one aspect, such correlations are determined from a model of the mechanism of action of a ErbB pathway-specific drug on an ErbB pathway. Preferably, methods of the invention are implemented by using sets of binding compounds having releasable molecular tags that are specific for multiple components of one or more complexes formed in ErbB pathway activation. After binding, molecular tags are released and separated from the assay mixture for analysis.10-16-2008
20130171679Micro-Incubation Systems for Microfluidic Cell Culture and Methods - A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems.07-04-2013
20130171682Cell Culture and Gradient Migration Assay Methods and Devices - A number of novel improved microfluidic configurations and systems and methods of manufacture and operation for a microfluidic invasion assay system.07-04-2013
20080248516Method for Using Division Arrested Cells in Screening Assays - Division arrested cells are used in screening assays to determine the effect of a substance of interest on the cells. The division arrested cells can be used in drug screening assays, signal transduction assays, and are especially useful in large scale, high throughput assays.10-09-2008
20090042239Particle Fusing Systems and Methods Using Acoustic Radiation Pressure - The present invention comprises methods and systems that use acoustic radiation pressure.02-12-2009
20080241874Method and device for manipulating individual small objects - A method for individually moving small objects, such as cells, from one location to another as well as a device for implementing the method is disclosed. A small object such as a cell is isolated at some initial location and moved to some destination location by the movement of the small object through a succession of intermediate locations until the small object arrives at the destination location. Also disclosed are methods of manipulating cells made possible by the method and device of the present invention.10-02-2008
20080241873MULTI-USE MULTIMODAL IMAGING CHELATES - Cyclen-based chelates can be used as contrast agents for multi-modal imaging of tissue cells. The cyclen-based chelates are preferably polyazamacrocyclic molecules formed from 1,4,7,10 tetraazacyclododecane (“cyclen”) having varying chelating ions, phosphoester chains, and light harvesting moieties. By changing the chelating ion, phosphoester chain length and/or the light harvesting moiety different imaging techniques, such as MRI, CT, fluorescence and absorption, x-ray and NIR, may be employed to image the tissue cells. Additionally, the cyclen-based chelates may be conjugated to provide for site-specific delivery of the cyclen-based chelate to the desired tissue cells. The cyclen-based chelates may also be delivered to the tissue cells by attaching the cyclen-based to a polymeric delivery vehicle. Although these cyclen-based chelates have a wide variety of application, the preferred use is for imaging of cancer cells, such as brain cancer, for improving resection of a cancerous tissue.10-02-2008
20080241872METHODS OF MODULATING COLD SENSORY PERCEPTION - The present invention relates to regulation of cold sensation and pain. More particularly, the present invention is directed to nucleic acids encoding a member of the transient regulatory protein family, CMR1, which is involved in modulation of the perception of cold sensations and pain. The invention further relates to methods for identifying and using agents that modulate cold responses and pain responses stimulated by cold via modulation of CMR1 and CMR1-related signal transduction.10-02-2008
20080241871Luminescence measuring apparatus - The present invention provides a luminescence measuring method that can be accurately and quickly carried out while inhibiting a possible background associated with viable bacteria adhering to a nozzle or Adenosine Tri Phosphate remaining in the nozzle, and an apparatus for the method. The present invention uses a washing apparatus characterized by including a nozzle, a lysys solution, a luminescence reagent solution, and a detection section, as well as a relevant washing method and a relevant luminescence measuring method. To remove viable bacteria adhering to the nozzle, the nozzle is immersed in the lysys solution and then in the luminescence reagent solution. The detection section monitors luminescence occurring during a washing process.10-02-2008
20080241870Composition For Creating an Artificial Bone Marrow Like Environment and Use Thereof - The present invention is in the domain of cell biology and medicine and relates to composition and in vitro methods for creation of artificial bone-marrow like environment and uses thereof.10-02-2008
20080233610SOMATIC CELL REPROGRAMMING - The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.09-25-2008
20080233609Spirolactam Targeting Compounds and Related Compounds - Spirolactam targeting compounds, related compounds, uses of such compounds, and methods of making such compounds are disclosed.09-25-2008
20080233608GENES AND PROTEINS INVOLVED IN LIPID REMODELING OF GPI-ANCHORED PROTEINS AND THE USE THEREOF - This invention provides proteins and genes thereof involved in the GPI lipid remodeling process and, thereby and constructing a system for screening for useful substances such as anticancer agents and a system for detecting abnormalities in the GPI lipid remodeling process.09-25-2008
20080233607Cell Culture Device - The invention provides cell culture devices comprising a channel, the channel comprising one or more inlets and one or more outlets, and a cell retention chamber defined by an internal surface of the channel and a plurality of projections extending therefrom. The invention further provides methods of use relating to such cell culture devices.09-25-2008
20090029403Sesnsor for detecting a toxic or hazardous gas mixture and operating method - A sensor for detecting a gas mixture which substantially comprises air and contains one or a plurality of gases that exhibit a disadvantageous effect on living organisms, comprising: a sensor chip composed of silicon for reading out at least one signal which is generated at a sensitive substance given the presence of one or a plurality of target gases in the measurement gas, a sensitive substance applied on the sensor chip, comprising living cells, which respond to target gas, a signal processing unit for evaluating the signals of the Si chip.01-29-2009
20080227140TRP/HIS Exchange and Kynurenine Induced TRP Transport - The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.09-18-2008
20080227139SYSTEM, METHOD AND APPLICATIONS INVOLVING IDENTIFICATION OF BIOLOGICAL CIRCUITS SUCH AS NEUROLOGICAL CHARACTERISTICS - Various aspects are directed to systems and methods for assessing neural activity of a neural region having multiple subfields. In certain embodiments, a method includes evoking a cellular electrical response in at least one subfield due to neural activity in the neural region, capturing image data of the electrical response at a level sufficiently detailed in space and time to differentiate between polarization-based events of two respective portions of the subfield, and then assessing neural activity by correlating space and time information, from the captured data, for the two respective portions of the sub-field. Other more specific aspects of the invention involve different preparation and neural stimulation approaches which can vary depending on the application.09-18-2008
20080227138Methods to Increase or Decrease Bone Density - The SOST gene gives rise to sclerostin, a protein that leads to apoptosis of bone progenitor cells. The invention provides antagonists to the sclerostin protein, and methods for identifying new sclerostin antagonists. The invention also provides molecules that can depress expression of the SOST gene, as well as methods for identifying such molecules. Such molecules and antagonists are useful for increasing bone mineralization in mammals, for example, in the treatment of osteoporosis.09-18-2008
20080227137METHOD OF IN VITRO DIFFERENTIATION OF NEURAL STEM CELLS, MOTOR NEURONS AND DOPAMINE NEURONS FROM PRIMATE EMBRYONIC STEM CELLS - A method of differentiating embryonic stem cells into ventral spinal progenitor cells is disclosed. In one embodiment, the invention comprises culturing a population of cells comprising a majority of cells that are characterized by an early rosette morphology and are Sox109-18-2008
20080227136Cell culture improvements - The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.09-18-2008
20110262959METHOD FOR EVALUATION OF DIFFERENTIATION ABILITY OF STEM CELL - The present invention provides a method of evaluating the possibility of a stem cell being able to differentiate into a cell that constitutes a desired tissue in a living organism, comprising the following steps of: 10-27-2011
20110262957COMPOSITIONS FOR THE DETECTION OF INTRACELLULAR BACTERIAL TARGETS AND OTHER INTRACELLULAR MICORORGANISM TARGETS - Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in added form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).10-27-2011
20110262952COLLECTION DEVICE AND MATERIAL - Swabs, and materials of the present disclosure, and methods of making same, include randomly arranged sea-island bicomponent fibers which have randomly splayed terminal ends.10-27-2011
20080241869Compositions and methods for ameliorating hyperlipidemia - The invention provides compositions and methods for treating hyperlipidemia by administering a Microsomal Triglyceride Transfer Protein (MTP) inhibitor in combination with a Liver Fatty Acid-Binding Protein (L-FABP) inhibitor, methods of preventing the development of hepatic steatosis, methods of identifying an agent useful for treating hyperlipidemia, and methods of screening for inhibitors of MTP and L-FABP activity. Also provided are pharmaceutical compositions comprising a MTP inhibitor and a L-FABP inhibitor.10-02-2008
20080220464BLOOD TEST INSTRUMENT USING A DISPOSABLE CARTRIDGE - A blood test instrument using a disposable cartridge and a method of measuring a blood sample using the instrument are disclosed. The instrument includes a cell counting station for counting blood cells by electrical resistance measurement, a pressure actuating component adapted to apply a pressure alternately on two flexible receptacles of a disposable cartridge removably placed in the instrument to cause flowing of a mixture of a blood sample and a liquid agent between the two receptacles to obtain proper mixing, and a conduit adapted to deliver the mixture to the cell counting station for counting. After measuring the blood sample, the instrument withdraws a washing liquid contained in another receptacle of the disposable cartridge and uses the washing liquid to clean the instrument and to deliver the mixture back to the cartridge for disposal.09-11-2008
20080220463METHOD OF CELL CHROMATOGRAPHY - A method is provided of conducting cell chromatography with a group of cells. Each cell has a fluorescence intensity. The method includes the step of depositing the group of cells into a first chamber for a first predetermined time period such that a first portion of cells of the group of cells attaches to a first surface. The cells unattached to the first surface are removed from the first chamber and deposited into a second chamber for a second predetermined time period. A second portion of cells of the unattached cells attach to a second surface. The cells unattached to the second surface are removed from the second chamber. Thereafter, the fluorescence intensities of the cells attached to the first and second surfaces are compared to a standard.09-11-2008
20090233327Surface Modification in a Manipulation Chamber - A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.09-17-2009
20130143254CO-CULTURE DEVICE ASSEMBLY - An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.06-06-2013
20130143255ILLUMINATION SIMULATOR FOR ALGAE GROWTH - Systems and methods are provided for using a growth vessel to simulate algae growth and/or productivity in a reference environment, such as an open pond, a closed photobioreactor, or a hybrid system. Based on predicted algae sample trajectories in the reference environment, an illumination profile is developed. An algae sample in the growth vessel can then be exposed to the illumination profile under controlled conditions. Properties of algae in the reference environment can then be characterized based on the sample exposed to the illumination profile.06-06-2013
20130143256LIQUID-BASED METHOD FOR PRODUCING PLANT EMBRYOS - The present invention relates to methods for developing embryos and producing germination-competent embryos using a liquid embryo development media.06-06-2013
20130143257Pressure Monitoring Of Whole Blood Aspirations To Determine Completeness Of Whole Blood Mixing - Methods for use with chemical analyzers aspirate a sample portion from one location to dispense it at a second location, aspirate another sample portion at that second location and dispense it at the first location, and measure the pressure values experienced inside a probe performing the aspirations and dispenses. By comparing the pressure values (or other values indicative of the viscosity or other relevant properties of the sample), the chemical analyzer can determine if the sample is sufficiently mixed or if the sample components remain separated and the method should be repeated.06-06-2013
20130171680CHIMERIC AVIAN-BASED SCREENING SYSTEM CONTAINING MAMMALIAN GRAFTS - The present invention relates to animal model systems comprising a chimera between an avian embryo and a mammalian organism. Specifically, chimeric model systems comprising normal, diseased or genetically transformed mammalian cells and tissues transplanted into avian embryos, and uses thereof for in vivo testing of drugs and therapeutic modalities are disclosed.07-04-2013
20130171681BLOOD CELL ANALYZER, BLOOD CELL ANALYZING METHOD, AND NON-TRANSITORY STORAGE MEDIUM - The present invention is a blood cell analyzer. When only measuring one or more measurement items included in one group, a first sample supplying operation is performed which includes aspirating a blood sample by a first amount. When measuring one or more measurement items included in the one group and another measurement item not included in the one group, a second sample supplying operation and a third sample supplying operation are performed. The second sample supplying operation includes aspirating the blood sample by the first amount for the measurement items included in the one group, and the third sample supplying operation includes aspirating the blood sample by a second amount for the another measurement item.07-04-2013
20120252054SYSTEM FOR TRANSFORMATION OF THE CHLOROPLAST GENOME OF SCENEDESMUS SP. AND DUNALIELLA SP. - The present disclosure relates to methods of transforming various species of algae, for example, algae from the genus 10-04-2012
20120252053REAGENTS AND METHODS AND SYSTEMS USING THEM - Certain embodiments described herein are directed to a reagent that includes an effective amount of an adsorber to remove interfering species present during heavy metal level measurement in a fluid sample. In some examples, the reagent can include an effective amount of an adsorber to remove a suitable amount of glutathione from the fluid sample such that the glutathione does not interfere with measurements of lead levels in the fluid sample.10-04-2012
20120252052METHOD FOR MULTIPLE QUANTIFICATION OF AMINO GROUP-CONTAINING NON-PEPTIDIC COMPOUND WITH HIGH EFFICIENCY AND HIGH SENSITIVITY AND KIT THEREFOR - A method of quantifying a target non-peptidic compound having an amino group contained in one or more biological samples, which comprises a step of producing a difference in the mass of the target non-peptidic compound between samples, by using a combination of two or more kinds of stable isotopes of a compound represented by the formula (I):10-04-2012
20120252050METHODS FOR ASSESSING RISK OF BONE FRACTURE - The invention generally relates to methods for assessing risk of bone fracture. In certain embodiments, methods of the invention involve a) conducting an assay to determine a characteristic of keratinized tissue obtained from a mammal, b) analyzing at least one bone-fracture risk factor associated with the mammal, and c) correlating results from steps (a) and (b), thereby assessing the risk of bone fracture of a bone of the mammal.10-04-2012
20110269172Micro-Electrode Grid Array for Top and Bottom Recording from Samples - A mixed micro-fluidic multi-electrode grid array (MEGA) device (11-03-2011
20110269169PSEUDOMONAS ALCALIPHILA MBR AND ITS APPLICATION IN BIOREDUCTION AND BIOSORPTION - The present invention relates to microbiology technology field. More particularly, it relates to 11-03-2011
20110269168Processes and kits for determining multi-drug resistance of cells - This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and provided.11-03-2011
20130177934Aminated Mesoporous Silica Nanoparticles, Methods of Making Same, and Uses Thereof - A mesoporous silica particle having 10 mole % to 65 mole % amine groups present in the silica of the particle and on the silica surface of the particle. The particle has Pm 07-11-2013
20130177935METHOD AND DEVICE FOR HIGH THROUGHPUT CELL DEFORMABILITY MEASUREMENTS - A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.07-11-2013
20130115648METHOD OF IDENTIFYING PREBIOTICS AND COMPOSITIONS CONTAINING THE SAME - A high-throughput, tiered screening method of indentifying test agents that exhibit prebiotic activity on human skin commensal microorganisms and cosmetic compositions that include such agents. The method includes determining the metabolite level of a culture and comparing the metabolite level to a control value. The assay further comprises performing a plate count using the culture when the metabolite level of the culture is greater than its corresponding control value. The plate count results are compared to another control value, and the test agent is identified as a prebiotic agent when the number of colonies present in the plate count assay of the culture is greater than the second control value.05-09-2013
20130115649Methods and Reagents for Metabolomics and Histology in a Biological Sample and a Kit for the Same - A method of extracting and measuring one or more biochemicals from a biological sample, comprises immersing the biological sample in an organic solvent, whereby one or more biochemicals present in the biological sample are extracted into the organic solvent; separating the biological sample from the free organic solvent; and measuring the level(s) of the one or more biochemicals extracted into the organic solvent, wherein the biological sample remains analyzable by histological analysis.05-09-2013
20130171683FLOW CYTOMETER APPARATUS AND METHOD - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.07-04-2013
20130095516Light Refraction Imaging to Measure Liquid Volume - A method to measure the volume of fluid present that relies on the pattern of light refraction as it passes though an airway surface liquid (ASL) meniscus. The method comprises allowing epithelial cells to grow on a membrane in a well so as to form a fluid meniscus about the perimeter of the well. The well is then illuminated by a light source. The illuminated cells in the well are then optically imaged by a scanner, a flat bed optical scanner, a camera, or any device capable of imaging. Microscopy may be used but is not needed, and high powered microscopy is certainly not needed. Then the imaging information from the cells in the well is used to determine a property of the meniscus in the well. Specifically, the imaging information can be analyzed to determine the dimensions, shape, and/or volume of the meniscus and the fluid in the well.04-18-2013
20130115651FLUORESCENT DYES - The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties.05-09-2013
20130102020Detection and Characterization of Protein Interactions, Protein Interaction Modulation and Protein Interaction Modulators - Protein interactions, protein interaction modulation, and protein interaction modulators can be detected and characterized through assessment of differential angular mobility and/or differential polarity exhibited by protein interaction reactants and products.04-25-2013
20130102021FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE - A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:04-25-2013
20130102023METHODS AND COMPOSITIONS FOR GROWTH OF CELLS AND EMBRYONIC TISSUE ON A SYNTHETIC POLYMER MATRIX - The present invention provides methods and compositions for establishing and maintaining growth of cells and embryonic tissue on a synthetic polymer matrix. For example, the present invention provides synthetic growth matrices for stem cells, gametes, mature differentiated cells, and embryonic tissue (e.g., blastomeres, embryos, and embryoid bodies). In certain embodiments, the cells are capable of going through multiple passages while remaining in an undifferentiated state as a result of the synthetic polymer matrix.04-25-2013
20130102022PLANTS WITH ALTERED CELL WALL BIOSYNTHESIS AND METHODS OF USE - Provided herein are plants having altered expression of a GAUT polypeptide. Such plants have phenotypes that may include decreased recalcitrance, increased growth, decreased lignin content, or a combination thereof. Also provided herein are methods of making and using such plants.04-25-2013
20130115650Isotopic Biomarkers for Rapid Assessment of Bone Mineral Balance in Biomedical Applications - The present invention relates to the use of natural isotopes 05-09-2013
20130130299ELECTROCHEMICAL FLOW CYTOMETRY - A method for triggering cellular exocytosis events and measuring quantal release of electroactive chemicals is disclosed. A method can include flowing a cell through a single cell channel having a pair of electrodes oriented to direct current through the cell at a stimulation location along the single cell channel. The pair of electrodes include a working electrode, a counter electrode and an optional reference electrode. The single cell channel has dimensions which provide direct contact of the cell with walls of the single cell channel sufficient to substantially reduce or eliminate shunt paths around the cell at the stimulation location. The cell can be exposed to an electric field at the stimulation location using the pair of electrodes sufficient to trigger exocytosis. Changes in current due to exocytosis processes appear as spikes that can be correlated with a quanta of the electroactive chemicals which are released during exocytosis.05-23-2013
20130130298BLOOD PRODUCT MANAGEMENT METHOD USING RBC DEFORMABILITY-BASED METRICS - A method for using red blood cell deformability testing to improve management of blood product comprising RBC, the method comprising: generating deformability data for red blood cells corresponding to a respective unit of blood product; correlating the deformability data with red blood cell viability or efficacy based on any available direct or indirect in vivo performance data; obtaining a representation of quality for the respective unit of blood product; and based on the representation of quality assigning a relative rank to and/or timing a transfer of the respective unit in an inventory of stored blood product units.05-23-2013
20130130296Modular Functional Peptides for Delivery of Nanoparticles - A peptide directs nanoparticles (such as quantum dots) to the plasma membrane of mammalian cells. A method of delivery of a nanoparticle to a plasma membrane of a cell includes providing to the cell a nanoparticle attached to a peptide configured to direct the nanoparticle the plasma membrane, and allowing the cell to take up the nanoparticle. The nanoparticle can be a FRET donor to an organic dye.05-23-2013
20130130295CARBON NANOTUBE BASED IMAGING AGENTS - Compositions and methods related to carbon nanotubes are provided. More particularly, imaging agents comprising carbon nanotubes internally loaded with a contrast agent and associated methods are provided. One example of a method may involve a method for imaging comprising: providing an imaging agent comprising a carbon nanotube loaded with contrast agent; introducing the imaging agent into a cell; and imaging the cell to detect the presence of the imaging agent.05-23-2013
20130130297INDUCTION OF A MATURE HEPATOCYTE PHENOTYPE - The present invention relates to a method for producing cells having a mature hepatocyte phenotype, comprising (a) providing a cell population comprising precursor cells of mature hepatocytes, wherein said cell population is obtained from a subject or derived from a cell line; and (b) introducing into said precursor cells a group of differentiation factors and/or the nucleic acid sequences encoding said differentiation factors, said group consisting of (i) one or more member(s) of the Foxa subfamily, (ii) HNF-4α and (iii) C/EBPα, thereby differentiating said precursor cells into cells having a mature hepatocyte phenotype.05-23-2013
20110217727METHOD FOR PATTERNING MAGNETIC MATERIALS IN LIVE CELL, METHOD FOR IMAGING PATTERN OF MAGNETIC MATERIALS, AND APPARATUS USED FOR SAME - The present invention provides a method for imaging a pattern of magnetic materials in a live cell comprising: preparing a plurality of magnetic materials magnetized in a direction of a line of magnetic force by applying a magnetic field, at least one of the magnetic materials being configured into a nanoparticle and the surface of at least one of the magnetic materials being modified; introducing a plurality of the magnetic materials into each live cell; providing the live cell with a label capable of binding to the magnetic material and imaging a pattern of the magnetic materials in a direction of a line of magnetic force; allowing a bundle of the lines of magnetic force to pass through the live cell in a direction by applying a focused magnetic field to the live cell; aligning a plurality of the magnetic materials in the live cell with the direction of the line of magnetic force by the applied magnetic field; and identifying an imaged pattern of the label capable of imaging the aligned pattern of the magnetic materials.09-08-2011
20110217723EPI-ILLUMINATION APPARATUS AND METHODS FOR SORTING PARTICLES - A method and apparatus for sorting stained particles in a fluid stream, each of the method and apparatus including an epi-illumination optics system with a focusing lens. The optics system being operable to direct a beam of electromagnetic radiation through the focusing lens in a forward direction along a beam axis intersecting particles in the fluid stream at said first location so that said particles pass through the beam, resulting in the fluorescence emissions from the particles being directed along said beam axis in a rearward direction.09-08-2011
20100047841SYNTHESIS OF DESACETOXYTUBULYSIN H AND ANALOGS THEREOF - Compounds of formula I, XVI and XXI possess potent cell growth inhibitory activity. These compounds are described have therapeutic utility, particularly in the treatment of cancer as well as conditions and disorders related to uncontrolled cell growth:02-25-2010
20110212484In Vitro Model of Focal Ischemia - A method of modeling in vitro focal ischemia comprising: perfusing a tissue slice in vitro with an oxygenated medium; and, applying a focal insult to a targeted portion of the tissue slice. The method is particularly useful for brain tissue.09-01-2011
20080199902Biosensor For Determining The Biochemical Oxygen Demand (Bod) By Respirometry - A removable cartridge that contains an adjustable number of capsules inside of which there are adjustable masses of immobilized microorganisms, allowing the design of a simple, portable apparatus and the use of a reactor with less priming time. A circulating pump injects maintenance water into the reactor, and the used maintenance water is pumped out to a disposal location or device by the same pump. An air pump injects air into the maintenance liquid and the excess air is released through an airflow outlet. A dissolved oxygen sensor is used to take several measurements of the dissolved oxygen content of the sample water, and various formulas are used to analyze the results. Reactors of this type can be stored in cold allowing the replacement of the operating reactor easily.08-21-2008
20080199901METHOD OF MONITORING MICROBIOLOGICAL ACTIVITY IN PROCESS STREAMS - An apparatus and method for monitoring microbiological activity in a process stream by measuring dissolved oxygen is disclosed. Bulk microbiological activity and surface associated biological activity are measured using this apparatus and method.08-21-2008
20080199900Disposable Device For One Or More Introductions, Treatment And Sampling Of Biological Material From At Least One Of The Separation Phases Present Within The Device, Under Sterility Conditions and Constant Pressure - A disposable device (08-21-2008
20110223630METHOD FOR SCREENING FOR COMPOUNDS THAT INHIBIT NEURODEGENERATION - Methods for screening for compounds that inhibit neurodegeneration are presented. Shedding of APP can be a useful marker for neurodegeneration and compounds that inhibit shedding of APP are useful as inhibitors of neurodegeneration. Such compounds may be useful in treatment and/or prevention of various neurological diseases, disorders and neuronal damage and may enhance growth, regeneration or survival of mammalian neuronal cells or tissue.09-15-2011
20130203101METHOD AND SYSTEM FOR PURIFYING AND QUANTITATING PROTEINS USING HEME FUSION TAGS - A method is provided for purifying a protein comprising the steps of providing a heme tag with an open coordination site and tagging the recombinant protein of interest with the heme tag. A resin framework is used, wherein a base that binds to heme is immobilized to the resin, and the open coordination site of the heme tag is capable of reversibly binding to the base immobilized to the resin. The tagged protein is reversibly bound to the resin, then eluted from the resin and quantified. The method enables the tagged protein to be tracked during protein expression or purification, and can be used to identify secretion of a protein to the periplasm or to tag proteins in the cytoplasm.08-08-2013
20130203102Device and Method for Transporting and Preparing Cellular Material - Embodiments of the invention relate to a device for transporting and preparing cellular material in a fluid for subsequent diagnostic purposes, comprising a cylinder having a fluid-tight screw-on cap and a fluid-tight base part that can be screwed off of the cylinder. The cap can be screwed onto the base part in a fluid-tight matter. The device provides for introducing cellular material to be diagnosed into a fluid in the cylinder, closing the cylinder by a cap for transport, removing the cap, introducing a filter device and concentrating the cellular material on a filter surface, after which the fluid level is at that part of the base part below the connection to the cylinder, disposing of the filtrate, removing the base part, pressing the filter surface onto an object carrier for a cell analysis, and closing the base part by the cap for further transport.08-08-2013
20130203103INFORMATION NOTIFICATION SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING - A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing.08-08-2013
20130203104FLUORESCENT PROTEINS WITH INCREASED ACTIVITY IN CELLS - The present invention relates to fluorescent proteins, in particular green fluorescent proteins (GFPs), with increased activity in cells, and thus increased signal strength. A further aspect of the present invention relates to the use of peptides for increasing the expression and/or stability of a protein in a cell.08-08-2013
20130203105METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method.08-08-2013
20110229928DEVICE AND METHOD FOR MICROWAVE ASSISTED CRYO-SAMPLE PROCESSING - Embodiments are provided that provide for devices and methods for microwave-assisted cryo-sample processing. In some embodiments, a system for microwave-assisted cryo-sample processing of a sample includes a chamber adapted to receive microwave radiation and a device disposed in the chamber that is configured to maintain a sample under cryo conditions during irradiation of the sample with microwave radiation.09-22-2011
20130137132CARDIOMYOCYTE CONTAINING DEVICE, MANUFACTURING METHOD AND MEASURING METHOD - Disclosed is a device (05-30-2013
20110244501CANCER STEM CELLS - Cancer stem cell populations characterized by expression of CD4410-06-2011
20130149731APPARATUS FOR CULTURING CELL AND ANALYZING CELL REACTION, AND METHOD OF ANALYZING CELL REACTION USING THE SAME - Provided is an apparatus for culturing a cell and analyzing a cell reaction. The apparatus may include a plurality of cell culturing rooms, cell injection ports connected to the cell culturing rooms through cell injection channels, respectively, and used for injecting different cells from each other into the cell culturing rooms, the number of the cell injection ports being the same as that of the cell culturing rooms, and a reagent injection port to inject a reagent into the cell culturing rooms.06-13-2013
20130149732METHOD OF LABELING SULFENIC ACID-CONTAINING PROTEINS AND PEPTIDES - A method of labeling a sulfenic acid (—SOH) group of a cysteine residue in a protein; or peptide, comprises contacting said protein or peptide with a beta-ketoester to covalently couple said beta-ketoester to said cysteine residue and form a beta-ketoester-labeled cysteine residue in said protein or peptide.06-13-2013
20130149733ESTABLISHMENT OF RHESUS MONKEY MODEL OF AUTOIMMUNITY TYPE 1 DIABETES - Use of low dose streptozocin in the preparation of an animal model for screening drugs for treatment of antoimmune type 1 diabetes is disclosed, in which streptozocin is administrated intravenously at a dose of 15-30 mg/kg per time for 5 days and administrated again on the 706-13-2013
20130149734Multi-photon Tissue Imaging - A multimodal method for imaging tissue comprising: aligning an excitation light source with at least a portion of the tissue; selecting at least two modalities of image acquisition; imaging the tissue portion with each of the modalities of image acquisition; and constructing a dual mode image using images from each of the modalities of image acquisition. A multimodal system for imaging tissue comprising: an excitation light source or light sources; an optical and alignment system for directing the excitation beam or beams to a sample and receiving an emission beam from the sample; at least one detector for receiving the emission beam from the sample; and a spectral filtering or dispersing device for providing at least two imaging modalities at the at least one detector; and a processor for analyzing the detected emission beam and constructing a dual mode image using images from each of the modalities of image acquisition.06-13-2013
20130149735MICROFLUIDIC ASSAY IN IDEALIZED MICROVASCULAR NETWORK FOR CHARACTERIZATION OF LEUKOCYTE ADHESION CASCADE - Methods of assaying the leukocyte adhesion cascade (LAC) and monitoring leukocyte rolling, adhesion, and/or migration can be implemented with an apparatus that includes an idealized microvascular network (IMN) of one or more interconnected idealized flow channels in fluid communication through a porous wall with a tissue space (e.g., idealized tissue space). The methods of assaying the LAC can be implemented with means for quantifying modulation of the leukocyte adhesion cascade. Methods of assaying the LAC can be implemented with the device and one or more active agents to monitor leukocyte rolling, adhesion, and/or migration in the presence of absence of the active agent. Migration can be through the idealized flow channels, through the porous wall, and/or into the tissue space.06-13-2013
20110236922METHOD TO DETERMINE STATE OF A CELL EXCHANGING METABOLITES WITH A FLUID MEDIUM BY ANALYZING THE METABOLITES IN THE FLUID MEDIUM - The present invention relates to a method for determining the ideal time for and outcome of reproductive health procedures including in vitro fertilization by establishing a correlation between the successful outcome of said procedure and the spectra of a body fluid obtained using a chosen analytical modality for a population of patients, acquiring for a patient a spectrum of the body fluid of the patient using said chosen modality.09-29-2011
20120276572METHODS AND COMPOSITIONS FOR IDENTIFYING AND VALIDATING MODULATORS OF CELL FATE - The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.11-01-2012
20110256582METHOD OF CLASSIFYING HUMAN SUBJECTS HAVING ADOLESCENT IDIOPATHIC SCOLIOSIS (AIS) AND METHOD FOR SCREENING FOR A COMPOUND USEFUL IN THE TREATMENT OF AIS AND RELATED SYNDROMES CAUSING SPINAL DEFORMITIES - A method of classifying a human subject having adolescent idiopathic scoliosis (AIS) comprising: providing a cell sample isolated from the subject; detecting an impairment in melatonin-signaling pathway in the sample in the presence and in the absence of a known melatonin-signaling pathway agonist, whereby the results of the detecting step enables the classification of the subject having AIS in one AIS subgroup; and a method of screening for a compound useful in the treatment of a disease characterized by a dysfunctional melatonin-signaling pathway, said method comprising the steps of contacting a candidate compound with at least one cell expressing at least one melatonin-signaling pathway impairment, wherein the candidate compound is selected if said melatonin-signaling pathway impairment is reduced in the presence of the candidate compound as compared to that in the absence thereof.10-20-2011
20130149736METHODS FOR SORTING PARTICLES - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.06-13-2013
20110275111MICROFLUIDIC DEVICE FOR FULL BLOOD COUNT - The present invention provides a microfluidic device (11-10-2011
20110275110METHOD FOR ISOLATING OR COUNTING MICROORGANISMS ON AN AGAR CULTURE MEDIUM - The invention relates to a method for isolating microorganisms on an agar culture medium, including the steps of: 11-10-2011
20110275109Immunity Evaluation Method, Apparatus and Program - An immunity evaluation method, apparatus, and program are provided that can evaluate comprehensive immunity with high precision. An immunity evaluation method for evaluating immunity from collected blood includes measuring a number of specific T cells that are CD8 positive and CD28 positive or negative in the collected blood, and determining a T lymphocyte age based on a regression equation on the basis of a correlation between a specific parameter that is dependent on the number of the specific T cells and age, and the number of specific T cells thus measured.11-10-2011
20110275108METHOD FOR RELEASING REDUCING GLYCAN BY AMMONIUM SALT - An objective of the present invention is to provide a glycan releasing method which can be applied to construction of a system for automation of glycan analysis, and particularly a glycan releasing method capable of analyzing an O-linked glycan. The objective could be achieved as a result of finding that the pH is lowered by using an ammonium salt or ammonium ion in the absence of concentrated aqueous ammonia, not using concentrated aqueous ammonia, thus drastically avoiding an undesired side reaction such as a peeling reaction or the like. Therefore, the present invention provides a method of producing an O-linked glycan from a glycan-binding substance having the O-linked glycan, the method includes the steps of (A) bringing an ammonium salt or ammonium ion into contact with the glycan-binding substance in the absence of concentrated aqueous ammonia (for example, under the conditions of the pH of about 7 or higher and lower than about 11); (B) neutralizing the reaction solution obtained in step (A); and (C) collecting the released glycan.11-10-2011
20110275107LONG WAVELENGTH ENGINEERED FLUORESCENT PROTEINS - Engineered fluorescent proteins, nucleic acids encoding them and methods of use.11-10-2011
20110275106DEVICE FOR MEASURING ACTIVITY OF CULTURED CELLS, MICROCHAMBER AND METHOD OF MEASURING ACTIVITY OF CULTURED CELLS - A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.11-10-2011
20110275105Methods of inducing pluripotency - Methods are provided for inducing non-pluripotent cells to become pluripotent. Methods also include identifying and isolating induced pluripotent (iPS) cells and uses thereof. Compositions and kits for carrying out the subject methods are also provided.11-10-2011
20100311106QUANTITATION OF LOBULAR INVOLUTION FOR BREAST CANCER RISK PREDICTION - Methods for determining risk of developing breast cancer are described.12-09-2010
20130183710Reference sample for quality control in molecular diagnosis - The present invention relates to a reference sample for quality control purposes in molecular diagnosis of biological samples, which reference sample comprises a mixture of cells from at least one reference cell line which exhibits a particular gene expression profile, and at least one type of lymphocytes. Furthermore, the invention relates to productions methods for such references samples as well as to methods of their use.07-18-2013
20100317050POLYPEPTIDES FOR MICROBIAL DETECTION - Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions.12-16-2010
20100317048NOVEL SYNTHETIC BLOCKING REAGENTS - The present invention concerns novel synthetic blocking reagents for the reduction of non-specific bindings in solid phase assays that rely on biological and specific recognition, e.g., in enzyme-linked immunosorbent assays (ELISAs). The invention provides the use of compounds as blocking reagents as well as kits comprising these compounds. The compounds comprise one or more poly(ethylene glycol) moieties, one or more alkyl- or aminoalkyl groups and another unit bridging the aforementioned groups, wherein the compound comprises at least one amino group.12-16-2010
20100317047Biomarkers of Hemorrhagic Shock - Methods for the use of claudin-3 as a biomarker for diagnosis and prognosis, and for monitoring the efficacy of treatment, in hemorrhagic shock (HS).12-16-2010
20100317045COMPOUNDS USED AS DYES COMPERABLE TO ALEXA FLUOR 350 DYES - Compounds used as dyes comperable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity.12-16-2010
20130157302METHOD FOR DETERMINING CAPABILITY OF A COMPOUND FOR INHIBITING A RECEPTOR USING CODON-OPTIMIZED NUCLEIC ACID - A method for determining a capability of a compound for inhibiting a receptor through a change of intracellular calcium ion when the receptor is activated is provided. Contacting a host cell genetically engineered to express an apo-clytin-II protein generated from a codon-optimized nucleic acid with a test compound, and then determining an amount of light generated. The intracellular calcium ion can be detected much more easily.06-20-2013
20130157303SULFATED POLYSACCHARIDE CAPABLE OF BINDING TO GROWTH FACTOR AND USE THEREOF - A biological substance which can enhance the proliferation and differentiation of cells; a method for evaluating the proliferation and differentiation of cells, which targets the biological substance; a composition for detecting the proliferation and differentiation of cells, which contains a molecule capable of binding to the biological substance; and a composition for enhancing the proliferation and differentiation of cells, which contains the biological substance. A part of a sulfated polysaccharide secreted from cells binds to a growth factor during the course of the proliferation and differentiation of the cells, that the amount of the sulfated polysaccharide capable of the above-mentioned binding and secreted from the cells correlates strongly with the progression of the proliferation and differentiation of the cells, and that the proliferation and differentiation of cells can be evaluated and the proliferation and differentiation of cells can be enhanced utilizing the sulfated polysaccharide.06-20-2013
20120282648METHOD AND SYSTEM FOR DETECTING AND GRADING PROSTATE CANCER - A method of analyzing a plurality of biopsy cores extracted from a plurality of respective biopsy locations in a prostate is disclosed. The method comprises: measuring a level of a chemical element in each of the biopsy cores, and generating a chemical element map of at least a portion of the prostate based on the levels and the respective biopsy locations. In some embodiments, the method determines at least one additional biopsy location for a future biopsy in the prostate.11-08-2012
20120282644MULTIPLE-ELECTRODE AND METAL-COATED PROBES - Provided are probes featuring multiple electrodes, which probes have diameters in the nanometer range and may be inserted into cells or other subjects so as to monitor an electrical characteristic of the subject. The probes may also include a conductive coating on at least one probe element to improve the probes' performance. The probes may also be used to inject a fluid or other agent into the subject and simultaneously monitor changes in the subject's electrical characteristics in response to the injection. Related methods of fabricating and of using the inventive probes are also provided.11-08-2012
20120282643CYAN AND YELLOW FLUORESCENT COLOR VARIANTS OF SPLIT GFP - Disclosed herein are Split-Fluorescent proteins (SFPs) including Split-Yellow Fluorescent Proteins and Split-Cyan Fluorescent proteins. Further disclosed are methods of using SFPs. For example, methods of identifying the subcellular localization of a protein and methods of identifying the membrane topology of a membrane protein are disclosed herein.11-08-2012
20120282647ASSAY FOR QUANTIFYING CLOSTRIDIAL NEUROTOXIN - Method of measuring an effect induced to a muscle tissue by a clostridial neurotoxin, comprising: 11-08-2012
20120282646METHOD FOR ACTIVATING A NATURAL KILLER CELL BY ADJUSTING THE EXPRESSION OF THE SOCS2 GENE - The present invention relates to a method for activating natural killer cells (NK cells), and more particularly, to a method for enhancing the cytotoxicity of natural killer cells by inducing the overexpression of suppressor of cytokine signaling 2 (SOCS2) which is a protein involved in cell-signaling pathways in natural killer cells. The inventors of the present invention observed that when natural killer cells were treated with IL-15, a cytokine involved in natural killer cell differentiation, the expression of SOCS2 increased and the expression of proline-rich tyrosine kinase 2 (Pyk2) was inhibited by the SOCS2, the expression of which increased. In addition, when Pyk2 was overexpressed, the ability to produce IFN-γ and the ability to kill tumor cells of natural killer cells decreased. Therefore, SOCS2 can be used for activating natural killer cells and the natural killer cells activated by the method can be used for the prevention or treatment of cancer.11-08-2012
20120282645NANOPARTICLES - Nanoparticles, compositions comprising the nanoparticles, and methods for manufacture and uses thereof are provided. In at least one specific embodiment, the nanoparticle can include at least one isotopically enriched metal oxide. The isotopically enriched metal oxide can be copper or zinc.11-08-2012
20130183709MATERIAL FOR SCREENING FOR COMPOUND ACTING ON ION CHANNEL AND USE THEREOF - An object of the present invention is to provide a screening system that targets ion channels and has superior efficiency. The present invention provides a material for screening for compounds that act on a target ion channel, comprising cells which retain at least one first DNA encoding a voltage-dependent Na ion channel that has been inhibited from being inactivated, and in which a K ion channel has been activated so that a resting membrane potential becomes deeper in a negative direction.07-18-2013
20130183708APPARATUS FOR EXECUTION OF TREATMENT OPERATIONS IN CONNECTION WITH COLOURING OF TISSUE SPECIMENS ON OBJECT GLASSES - An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus (07-18-2013
20130183707STEM CELL BIOINFORMATICS - Ex vivo cell culture, especially the culture of stem cells, is a valuable and widely used technique. The appearance of unlabeled cultured cells contains significant information about the cell's identity, including its differentiation status and lineage. However, mere visual inspection of cells is a subjective process subject to inconsistencies between microscopists. This disclosure provides methods of quantifying cells' appearance, validating identity with known biomarkers, allowing automated classification of cells as well as automated segmentation and delineation of the borders of a cell colony. Also provided are systems and methods for comparing and standardizing cells cultured by different scientists using different cell culture methods.07-18-2013
20130183706Method for Culturing Adipocytes - The present invention relates to a method for culturing adipocytes, in which a homogeneous population of mature adipocytes isolated from adipose tissue is cultured on a three-dimensional culture substrate.07-18-2013
20130183705CONVERSION OF ALGAE TO LIQUID METHANE, AND ASSOCIATED SYSTEMS AND METHODS - Systems and methods for converting algae to liquid methane are disclosed. The system in accordance with a particular embodiment includes an algae cultivator, an anaerobic digester operatively coupled to the algae cultivator to receive algae and produce biogas, and a biogas converter coupled to the anaerobic digester to receive the biogas and produce liquefied methane and thermal energy, at least a portion of the thermal energy resulting from a methane liquefaction process. The system can further include a thermal path between the biogas converter and at least one of the algae cultivator and the anaerobic digester. The system can still further include a controller coupled to the biogas converter and at least one of the algae cultivator and the anaerobic digester. The controller can be programmed with instructions that, when executed (e.g., based on measured variables of the system), direct the portion of thermal energy between the biogas converter and the algae cultivator and/or anaerobic digester.07-18-2013
20090197293NOVEL USE OF AN INDICATOR CELL LINE FOR BIOASSAY - The present invention relates to methods of bioassay for detecting the effective concentration of Granulocyte Colony Stimulating Factor (G-CSF), and estimating ED08-06-2009
20090197291Assays Using Nanoparticles - A method for quantitatively and qualitatively determining the presence of a macromolecule comprises providing nanoparticles in a buffered solution, adding a test sample to the buffered nanoparticle solution, and measuring the difference between the buffered nanoparticles in the presence and absence of the test sample. The nanoparticles are preferably less than 100 nm in size.08-06-2009
20090104649MARKERS FOR PREECLAMPSIA - This document provides methods and materials related to determining whether or not a pregnant mammal (e.g., a pregnant human) has preeclampsia. For example, methods and materials related to the use of urinary podocytes to determine whether or not a pregnant human has preeclampsia are provided.04-23-2009
20110312018Blood-Brain Barrier Model - A method of creating a multicellular blood-brain barrier model is disclosed. In one embodiment, the method comprises culturing primary brain microvascular endothelial cells or embryonic stem cell-derived endothelial cells upon a permeable support in the presence of neural progenitor cells.12-22-2011
20110312017DIAGNOSIS OF ENTRY OF GASTROINTESTINAL CONTENTS INTO RESPIRATORY TRACT OF HUMANS AND ANIMALS - A method of diagnosing respiratory fluid in a patient suffering from gastro esophageal reflux disease (GERD) is disclosed. The method comprises (1) orally administering to a subject suspected of suffering from gastro esophageal reflux disease (GERD), a formulation comprised of a plurality of particles comprised of a biocompatible polymer (e.g. carnauba wax) and a detectable, non-radioactive label (e.g. a fluorescent label), (2) allowing the formulation to remain in the subject over a period of time during which the subject would be expected to aspirate the formulation, (3) collecting respiratory fluid from the subject, and (4) analyzing the respiratory fluid to determine if the fluid contains the detectable label, and thereby determining if the subject regurgitates fluid into the respiratory tract. Alternatively, or additionally, a subject swallows a label that is not destroyed in the respiratory tract, or in the gastrointestinal tract, which further has the property that it is absorbed from the respiratory tract but not from the gastrointestinal tract. The presence of the label is then analyzed in blood, or, if the substance is excreted from blood to urine, in urine samples. The qualitative analysis can be also expanded into quantitative analysis, enabling the estimation of either the concentration, or the amount, or both, of the gastrointestinal contents that entered the respiratory tract.12-22-2011
20110312016Method for Evaluating Immunosuppression - Provided is a method for determining immunosuppression in an individual. The method entails obtaining a sample of blood from an individual, contacting cells in the blood sample with an activating agent to obtain activated cells, measuring the amount of nuclear NFkB in the activated cells, and comparing the amount of nuclear NFkB in the activated cells to a control. An amount of nuclear NFkB that is higher than the control is considered to be indicative of insufficient immunosuppression in the individual. An amount of nuclear NFkB that is lower than the control is considered to he indicative of excessive immunosuppression in the individual. An amount of nuclear NFkB that is the same as the control is considered to be indicative of an appropriate amount of immunosuppression in the individual.12-22-2011
20110312015RAPID THROMBOCHEK TEST KIT BASED ON WHOLE BLOOD SCREENING TEST TO DETECT PLATELET HYPERAGGREGATION AT A TEMPERATURE OF 37 C IN THE CLINICAL LABORATORY - The new RAPID THROMBOCHEK TEST KIT is based on thrombochek screening test for measurement of hyperaggregable platelets in whole blood, which may be present either in form of circulating platelet aggregates, spontaneously aggregating platelets or reacting to weak agonist of platelet aggregation in low concentration—all together, in order to investigate thrombotic tendencies and to assess the efficacy of antithrombotic therapy all measured with help of hematology cell counter and magnetic stirrer at 37° C. of temperature. This is compared to the other existing microscopic method and shows 0.97% correlation. RAPID THROMBOCHEK TEST KIT based on the above methodology is to be used with the aid of an automated hematology analyser and is made available for routine clinical and office laboratory use.12-22-2011
20110312014PROBE FOR DETECTION AND QUANTIFICATION OF NITRIC OXIDE, AND METHOD FOR DETECTING AND QUANTIFYING NITRIC OXIDE USING THE SAME12-22-2011
20110312013METHOD AND KIT FOR EXAMINATION OF CELLS USING N-(9-ACRIDINYL) MALEIMIDE (NAM) OR USING 7-DIETHYLAMINO-3-((4'-IODACETYL)AMINO)PHENYL)-4-METHYLKCOUMARIN (CPI) - The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI). The invention further relates to compositions used in methods for analyzing cells.12-22-2011
20110312012METHOD AND KIT FOR ASSESSING VIABLE CELLS - The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).12-22-2011
20130189722ISOLATION OF PELLET-FORMING MICROORGANISMS - The present disclosure provides technologies for identifying, characterizing and/or sorting pellet-forming microorganisms such as bacteria and/or fungi (e.g., yeast) in liquid culture, and particularly under fermentation conditions. In some embodiments, the pellet-forming microorganisms produce one or more commercial products. For example, in some embodiments the pellet-forming microorganisms produce one or more organic acids, carotenoid compounds, essential fatty acids, industrial enzymes, active pharmaceuticals, extracellular carbohydrates, and insecticidal compounds, etc. In many embodiments, the organisms are sorted while in their pellet form.07-25-2013
20130189723AUTOMATED CELL CULTURE SYSTEM AND PROCESS - The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and/or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and/or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrier manufacturing method, a monitoring method, a cell culture method, and an analytical method. Either a single bioreactor or a plurality of bioreactors are used in the automated system to enable cell culture and analysis with a minimum of human intervention.07-25-2013
20130189724USE OF AN ADAPTIVE CHEMICALLY REACTIVE PLASMA FOR PRODUCTION OF MICROBIAL DERIVED MATERIALS - A carbonaceous feedstock can be converted into a chemical product having a higher commercial value, by introducing the carbonaceous feedstock into a plasma torch under conditions selected to generate a synthetic gas mixture having a tailored composition, and then introducing the synthetic gas mixture into a microbial digester configured to convert the synthetic gas mixture into the chemical product. The composition of the synthetic gas mixture produced by the plasma torch can be quickly modified to yield an optimal quality and quantity required by the microbial digester, and the quantity and stoichiometric ratio can be quickly varied if the quantity or composition of the synthetic gas mixture being provided is not appropriate for the microbial digester, enabling greater efficiency to be achieved as compared to systems where the quality and quantity of the synthetic gas mixture cannot be easily changed.07-25-2013
20130189725HUMAN IN VITRO MODEL OF THE BLOOD CEREBROSPINAL FLUID BARRIER - The present invention relates to a human in vitro model system of the blood cerebrospinal fluid barrier (BCSFB), to a method for producing said model system, as well as to uses thereof.07-25-2013
20130189726METHOD FOR PREPARING AND/OR PROCESSING A BIOLOGICAL SAMPLE USING A MALODOUR COUNTERACTANT - The present invention pertains to a biotechnological method for preparing and/or processing a biological sample, in particular for isolating at least one target biomolecule therefrom, which characterised in that at least one malodour counteractant is used for preventing, reducing, masking and/or suppressing malodour and/or malodour formation during the preparation and/or processing of said biological sample.07-25-2013
20130189727Methods And Compositions For Cellular Imaging And Cancer Cell Detection Using Light Harvesting Conjugated Polymer-Biomolecular Conjugates - The invention is a compound represented by any one of Structural Formulas (I)-(IV), or a salt thereof, wherein the values and alternative values for the variables are as defined in the Detailed Description of the Invention. Methods using a compound of Structural Formula (I)-(IV), or a salt thereof, are also presented.07-25-2013
20130115647Method to Increase the Number of Detectable Photons During the Imaging of a Biological Marker - The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence; b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals and metalloporphyrins. This method is charcterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon(s) are of a longer wavelength than each of the at least one first photon(s).05-09-2013
20120015395Human Blood-Brain Barrier Endothelial Cells Derived From Pluripotent Stem Cells and Blood-Brain Barrier Model Thereof - A model blood brain barrier obtained from hPSCs is disclosed.01-19-2012
20120015393ENDOGENOUS AUTO-FLUORESCENT BIOLOGICAL MARKERS FOR ASSESSING A BIOLOGICAL PARAMETER OF A CELL - The present application relates to the use of endogenous fluorescent biological markers to determine a biological parameter of a cell in a liquid. The methods provided herein provide accurate results in a relatively short amount of time and can be used to monitor and optimize cell culture online as well as determining the presence of a cellular contamination in a cell suspension.01-19-2012
20120021452Belt Agitation System For Culture Bottles - A specimen culture container agitation assembly includes an endless belt having a multitude of container-receiving clips attached thereto, each of which is adapted for retaining a specimen culture container to the belt. The belt is oriented vertically. The assembly further includes upper and lower pulleys over which the belt is driven. A drive motor drives the belt around the pulleys. Agitation of the specimen culture container is achieved by rotation of the belt over the pulleys and the retaining of the specimen culture container by the clip.01-26-2012
20120021451SAMPLE ANALYSIS APPARATUS AND SAMPLE ANALYSIS METHOD - An analysis apparatus includes a plurality of separation channels, a detector, and a control unit. In each separation channel, a particular component contained in a sample is separated from other components. The detector detects the separated particular component. The control unit controls an analysis step and a preprocessing step. The analysis step includes a separation step of performing the separation and a detection step of performing the detection in each separation channel. The preprocessing step is performed to put each separation channel into a state in which it can perform the analysis step. The control unit simultaneously carries out at least parts of the preprocessing steps of at least two of the plurality of separation channels.01-26-2012
20120021450METHODS FOR MAGNETIC RESONANCE IMAGING - In certain aspects the present invention provides methods and compositions related to contrast agents for magnetic resonance imaging. In certain variations, contrast agents provided herein are generated in situ via genetic instructions and become potent upon sequestering available metal atoms. Exemplary contrast agents include metal-binding proteins.01-26-2012
20120021449DEVICE FOR AUTOMATICALLY ANALYZING MICROORGANISMS AND METHOD FOR AUTOMATICALLY ANALYZING MICROORGANISMS - After culturing blood, a culture liquid determined as positive is transplanted into a plate medium and a bacterial cell suspension that is directly usable for identifying and testing antibiotics-sensitivity is prepared without forming colonies. Provided are a device for automatically analyzing microorganisms and a method therefor whereby blood culture and an identification and antibiotics-sensitivity test can be continuously operated. A device for automatically analyzing microorganisms which is configured so that a blood culture test and an identification and antibiotics-sensitivity test can be automatically and continuously conducted, wherein means for pretreating a cultured blood sample comprises a mechanism for removing culture liquid components in the course of the blood culture and a mechanism for controlling the microbial concentration (bacterial cell count) to a constant level after the blood culture.01-26-2012
20120021448METHOD FOR EVALUATING STATE OF CELLS - Provided are a novel marker of the state of cells and the differentiation level of the same which is usable as a substitute for a gene or a protein; a means for quantitatively understanding this marker; and a means for understanding the state of specific cells and the differentiation level of the same using this marker. A method for determining a sugar chain category to be used for evaluating the state of cells. The quantitative profile of N-linked sugar chains of cells before a change in the state thereof is obtained. Next, the quantitative profile of N-linked sugar chains contained in the cells after the change in the state thereof is obtained. Sugar chains showing variations in the contents thereof between these two quantitative profiles are extracted and the individual sugar chains contained in the extracted sugar chains are classified into the following categories based on sugar chain type: either the high-mannose type or the non-high-mannose type; (1) the presence/absence of a bisect; (2) the number fucose residues (0, 1, or 2 or more); (3) the presence/absence of sialic acid; and (4) the degree of branching (2 or 3 or more). From these five categories, a sugar chain category appropriate for evaluating the state of cells is determined. A method for evaluating the state of cells and the differentiation of cells using the thus determined sugar chain category.01-26-2012
20120028293MIXING DEVICE AND MIXING METHOD FOR MIXING SMALL AMOUNTS OF LIQUID - The invention relates to a mixing method for mixing at least one small quantity of liquid, in which a quantity of liquid is applied, in a reaction region and at least one surface sound wave is reacted with the quantity of liquid. The invention relates further to a mixing device for mixing at least one quantity of liquid for performing the method of the present invention, a use of the device, and a method of analysis for bond strengths on surfaces.02-02-2012
20120028292METHODS FOR DIAGNOSING KIDNEY DAMAGE ASSOCIATED WITH HEART FAILURE - Disclosed is a method for diagnosing kidney damage in a subject suffering from heart failure including the steps of a) determining the amounts of liver-type fatty acid binding protein (L-FABP) and kidney injury molecule 1 (KIM-1) and optionally a natriuretic peptide in a sample of a subject, b) forming the L-FABP/KIM-1 ratio, c) comparing the amounts determined in step a) with reference amounts, and diagnosing the kidney damage. Also disclosed are a device and a kit for carrying out the method.02-02-2012
20120028291CHARGED-BALANCED IMAGING AGENTS - The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.02-02-2012
20120028290Compositions and Methods for Labeling and Imaging Phospholipids - The present invention provides a method to label phospholipids in vivo based on the metabolic incorporation of an alkynyl- or azido-labeled metabolic precursor into phospholipids. The resulting phospholipids have alkynyl or azido moieties, which, upon reaction with a labeled azide or alkyne, respectively, form labeled compounds that can be visualized using optical or electron microscopy with high sensitivity and spatial resolution in cells or tissue. The present method provides a valuable tool for imaging phospholipid synthesis, turnover and subcellular localization in cultured cells as well as in animals.02-02-2012
20120028289METHOD OF IDENTIFYING ENDOGENOUS FLUORESCENT BIOLOGICAL MARKERS FOR MONITORING CELLS - The present application is related to methods for the identification of endogenous fluorescent biological markers that generate information on cell culture parameters. Because the techniques provided herein provide accurate results in a relatively short amount of time, the methods described herein can be used to identify numerous endogenous fluorescent biological cell markers and monitor various cell culture conditions.02-02-2012
20120028288Method for Measuring Cell Motility and System Therefor - The present invention is intended to provide a technique whereby quantitative data of cellular chemotactic activity can be obtained. Namely, it relates to a method of measuring cell motility whereby the motility state of cells is measured. Living cells are placed in an observation space (02-02-2012
20130196362DIARYLAMINE-BASED FLUOROGENIC PROBES FOR DETECTION OF PEROXYNITRITE - Provided herein are improved fluorogenic compounds and probes that can be used as reagents for measuring, detecting and/or screening peroxynitrite. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. Also provided herein are fluorogenic compounds for selectively staining peroxynitrite in the mitochondria of living cells. Provided also herein are methods that can be used to measure, directly or indirectly, the presence and/or amount of peroxynitrite in chemical samples and biological samples such as cells and tissues in living organisms. Also provided are high-throughput screening methods for detecting or screening peroxynitrite or compounds that can increase or decrease the level of peroxynitrite in chemical and biological samples.08-01-2013
20130196363COLLECTION DEVICE AND MATERIAL - Swabs, and materials of the present disclosure, and methods of making same, include randomly arranged sea-island bicomponent fibers.08-01-2013
20130196361SPLIT SENSOR AND HOUSING ASSEMBLY FOR FLEXIBLE WALL - Disclosed is a sensor and housing assembly for a single-use bioreactor system, including a device for operatively associating a sensor with a flexible wall, the device comprising an internal housing portion that is removable and variably positionable on an internal surface of the flexible wall, the internal housing portion comprising a chemical detector sensor, which may be an optical chemical detector sensor.08-01-2013
20080286827BIOMARKERS - The invention relates to biomarkers for use in diagnosing cancer and in classifying tumors.11-20-2008
20100075361METHODS OF GENERATING FLORESCENCE RESONANCE ENERGY TRANSFER (FRET) BETWEEN SEMICONDUCTOR QUANTUM DOTS AND FLUORESCENT DYES/PROTEINS VIA MULTI-PHOTON EXCITATION, ACHIEVING ZERO BACKGROUND OR DIRECT EXCITATION CONTRIBUTIONS TO THE FRET SIGNATURE - A system and method of sensing physiological conditions in biological applications includes a laser source for optically exciting a plurality of luminescent quantum dots and a plurality of biomolecules in a nanoscale sensing system having a nanocrystal structure, where the plurality of biomolecules is stained with dye. In a multi-photon excitation process, a laser system optically excites, the plurality of luminescent quantum dots and the plurality of biomolecules in the nanoscale sensing system, where fluorescence resonance energy transfer (FRET) occurs between the plurality of quantum dots and the plurality of biomolecules. Stability of self assembly of quantum dot peptide conjugates within the plurality of biomolecules is investigated. Physiological conditions at the cellular level are determined, using a spectrometer to sense fluorosence spectra. The sensing of physiological conditions includes transducing signals into immunoassays, clinical diagnostics and cellular imaging to provide treatment to biological subjects including human patients.03-25-2010
20090075323Static Diffusion Cell for Diffusion Sampling Systems - A new design for a static diffusion cell for use in a diffusion sampling apparatus to be used in conjunction with automated or manual sampling is disclosed. The diffusion cell of present invention provides to an improved and efficient diffusion assay system.03-19-2009
20120070858METHOD FOR ISOLATING EXOSOMES FROM BIOLOGICAL SOLUTIONS USING IRON OXIDE NANOPARTICLES - A method for isolating exosomes from blood platelets using superparamagnetic nanoparticles of iron oxide (Fe03-22-2012
20130095517METHODS AND SYSTEMS FOR FLUID EXAMINATION AND REMEDIATION - A method for in situ monitoring within a specified environment. The method includes locating a housing in a well, wherein a set of pumps and a plurality of test beds are inserted. Each of the set of pumps are controlled by signals from the control system to push water from each pump into one of the plurality of separate test beds where, after flowing through each of the test beds, effluent flows into an effluent storage device.04-18-2013
20130095518METHOD FOR PRODUCING TUMOR CELLS FROM NORMAL MAMMARY EPITHELIAL CELLS - An object of the present invention is to provide a method for producing tumor cells from cells derived from normal cells without using hTERT. 04-18-2013
20120094322DEVICE FOR DIAGNOSIS OF PHYSIOLOGIC STATUS AND/OR SELECTION OF THE BEST SPERMATOZOA OF A SEMEN SAMPLE BASED ON CHEMOTAXIS, AND PROCEDURE OF USE THEREOF - A device for diagnosis of physiologic status and/or selection of best spermatozoa of a semen sample based on chemotaxis, and the procedure of thereof, enabling by a simple and inexpensive device the diagnosis and selection of the best spermatozoa in only one step. Only needed are: the present device, a regular light microscope, and personnel with elementary knowledge of laboratory management. The device is of the type having two communicated compartments (04-19-2012
20130210054Amino Acid Copolymer Assay - The present disclosure relates to polarized type 2 T helper (Th2) cells and methods for using such cells in the assessment of antigenic compositions.08-15-2013
20130210053MECHANOCHROMIC LUMINESCENT DIFLUOROBORON BETA-DIKETONATES - The invention provides luminescent solid-state compositions comprising difluoroboron beta-diketonates wherein the compositions can exhibit mechanochromic luminescence. The mechanochromic effect on the luminescence can be reversible, such as thermally reversible. Various solid-state forms of the invention can have emission spectra that differ from the properties of the respective difluoroboron beta-diketonate in solution. The mechanochromic effect can be stimulated by pressure such as hand-writing, and can be reversed over a period of minutes to hours at room temperature. The invention also provides methods of making and methods of using the solid-state compositions, such as for sensors and for information displays for use in biological sensing, and in art, design, and consumer products. Compositions of the invention, such as compositions in nanoparticulate form, or contained within a matrix material, can be used in conjunction with fluorescence microscopy to provide information concerning pressures and tensions within and external to living cells, tissues, or organisms.08-15-2013
20130210055SYSTEM AND METHOD FOR STUDYING EPIDERMIS SAMPLES EX VIVO - An epidermal sample is placed into a sample holder formed as a sandwich assembly. The sample holder is placed in an upper well within a lower well to be exposed to media in both wells. Properties of the sample can be studied, such as paracellular flux, transepidermal electric resistance, reaction to compounds, and epidermal barrier recovery.08-15-2013
20130210056APPARATUS FOR PRODUCING HIGH PURITY X-CHROMOSOME BEARING AND Y-CHROMOSOME BEARING POPULATIONS OF SPERMATOZOA - Isolated non-naturally occurring populations of spermatozoa (08-15-2013
20130210057USE OF LABEL-FREE BIOSENSORS TO UNDERSTAND AND IDENTIFY TREATMENT FOR CANCER - The disclosure relates to methods of using dynamic mass redistribution data obtained from cancer cells cultured on waveguide grating biosensors in the presence of agonists and in the presence of chemotherapeutic agents, for predicting effective chemotherapies for the treatment of cancer.08-15-2013
20130210058SYSTEM FOR NONINVASIVE DETERMINATION OF WATER IN TISSUE - An apparatus and method for non-invasive determination of hydration, hydration state, total body water, or water concentration by quantitative spectroscopy. The system includes subsystems optimized to contend with the complexities of the tissue spectroscopy, high signal-to-noise ratio and photometric accuracy requirements, tissue sampling errors, calibration maintenance, and calibration transfer. The subsystems include an illumination subsystem, a tissue sampling subsystem, a spectrometer subsystem, a data acquisition subsystem, a computing subsystem, and a calibration subsystem.08-15-2013
20130210059Method for Selecting Mevalonate Synthesis Modulators Using Cells Derived From Human Pluripotent Cells - A method for selecting pharmaceutical compounds affecting mevalonate or cholesterol, comprising a step for putting into contact with the pharmaceutical compounds to be tested, cells of the MSC type obtained by a method for producing cells of the MSC type from human pluripotent cells or from induced stem cells, comprising a step for cultivating human pluripotent cells or induced stem cells in a culture medium comprising: 08-15-2013
20130210060METHOD OF PRODUCING PANCREATIC HORMONE-PRODUCING CELLS - Disclosed is a production method of pancreatic hormone-producing cells in a form that mimics the pancreatogenesis, the method comprising subjecting stem cells to the following steps: (1) cultivating stem cells in a medium containing a Rho kinase inhibitor, (2) cultivating the cells obtained in (1) in a medium containing a GSK3 inhibitor, (3) cultivating the cells obtained in (2) in a medium containing GSK3 inhibitor and an activator of activin receptor-like kinase-4,7, (4) forming a cell mass from the cells obtained in (3), and cultivating the cell mass in a suspension state in a medium, (5) cultivating the cells obtained in (4) in a medium containing a retinoic acid receptor agonist, an inhibitor of AMP-activated protein kinase and/or activin receptor-like kinase-2,3,6, an inhibitor of activin receptor-like kinase-4,5,7 and a cell growth factor, and (6) cultivating the cells obtained in (5).08-15-2013
20130210061Method of Screening Damp-Dry Malodor Inhibitor and Method of Evaluating Damp-Dry Malodor Inhibitor - A method of screening a damp-dry malodor inhibitor, containing the steps of: bringing microorganisms having a 4-methyl-3-hexenoic acid production capacity into contact with a test substance in the presence of a sebaceous dirt component, detecting the production of a damp-dry malodor-causing substance by the microorganisms, and thereby selecting a test substance having a damp-dry malodor inhibitory function; and a method of evaluating a damp-dry malodor inhibitor, containing the steps of: bringing microorganisms having a 4-methyl-3-hexenoic acid production capacity into contact with a test substance in the presence of a sebaceous dirt component, detecting the production of a damp-dry malodor-causing substance by the microorganisms, and thereby evaluating the damp-dry malodor inhibitory function of the test substance.08-15-2013