Class / Patent application number | Description | Number of patent applications / Date published |
435026000 | Involving dehydrogenase | 67 |
20080248514 | ELECTROCHEMICAL METHOD FOR GLUCOSE QUANTIFICATION, GLUCOSE DEHYDROGENASE COMPOSITION, AND ELECTROCHEMICAL SENSOR FOR GLUCOSE MEASUREMENT - A method of quantifying glucose in a solution characterized in that electric potential measurement is conducted by potentiometry using a glucose dehydrogenase that requires a flavin compound as a coenzyme. It is preferable to carry out the quantification using a glucose dehydrogenase derived from a filamentous fungus, in particular derived from | 10-09-2008 |
20090035800 | Novel Use of Fluorescence Resonance Energy Transfer - A novel use of Fluorescence Resonance Energy Transfer wherein a labelled protein comprising a Fluorescent energy donor label and at least one energy acceptor moiety capable of accepting energy from the donor label by Förster energy transfer is exposed to incident electromagnetic energy to excite the donor moiety and the fluorescence emission of the donor is measured. The or each energy acceptor moiety has a more and less active energy acceptor state and the level of quenching of donor fluorescence is indicative of this state. The energy acceptor moiety may be converted between its states by a redox reaction, optionally involving a partner redox protein. A novel system comprising the labelled protein, a redox partner protein, ‘a light source for imposing incident light at the excitation wavelength for the fluorescent label and a light detector capable of detecting the fluorescence emitted by the label may be used in biosensors and/or to monitor enzymatic turnover. | 02-05-2009 |
20090087873 | SHARPLY RESOLVING LABELED PROTEIN MOLECULAR WEIGHT STANDARDS - Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced. | 04-02-2009 |
20090087874 | GLUCOSE DEHYDROGENASE AND PRODUCTION THEREOF - The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from | 04-02-2009 |
20090181417 | COLORIMETRIC MEASUREMENT OF ENZYMATIC ACTIVITY IN CELL LYSATES - The present invention concerns a method for the calorimetric measurement of enzyme activity comprising the following steps: preparing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction. | 07-16-2009 |
20090186372 | Reagent Compositions For Use In Electrochemical Detection - Biological reagent compositions with improved sensitivity to the concentration of blood glucose in patient samples for use in measuring systems and methods. The reagent compositions comprise a glucose oxidoreductase enzyme, a flavin nucleoside coenzyme and a mediator formulation. The mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex. | 07-23-2009 |
20090221017 | ANTI-AMYLOID BETA ACTIVITY OF INTRAVENOUS IMMUNOGLOBULIN (IVIG) IN VITRO - The present invention relates to a cell-based in vitro screening assay for identifying and selecting therapeutic agents that inhibit amyloid r-induced cytotoxicity. | 09-03-2009 |
20090246818 | COMPOUNDS, METHODS, COMPLEXES, APPARATUSES AND USES RELATING TO STABILE FORMS OF NAD/NADH - The invention concerns stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives, enzyme complexes of these derivatives and their use in biochemical detection methods and reagent matrices. | 10-01-2009 |
20090280516 | CRYSTAL STRUCTURE OF ALDEHYDE DEHYDROGENASE AND METHODS OF USE THEREOF - The present disclosure provides a crystal structure of aldehyde dehydrogenase (ALDH) with a modulator of ALDH bound thereto. The present disclosure provides a computer readable medium comprising atomic coordinates for an ALDH polypeptide and a modulator bound to a site within the polypeptide. A method is also provided. In general terms, the method comprises computationally identifying a compound that binds to an ALDH polypeptide, using the atomic coordinates. | 11-12-2009 |
20100047839 | LATENT FLUORIMETRIC INDICATOR FOR BIOLOGICAL ANALYTES DETERMINATION AND THE PREPARATION METHOD THEREOF - The present invention provides a sensitive fluorimetric indicator for analytes determination in the oxygen-insensitive DT-diaphorase-coupled dehydrogenases assay by omitting NADH, which is generated by reaction in the presence of analytes, which presents to the applicability as a biosensor for future clinical diagnostic. Furthermore, the novel long-wavelength latent fluorimetric indicator is also a user-friendly probe for monitoring DT-diaphorase activity. The fluorescence signal revealed by this process is specific and exhibited in the near red spectrum region. | 02-25-2010 |
20100086958 | NOVEL ENZYME - Novel FDH enzymes are provided herein, including novel NADPH-specific and NADH-specific FDH enzymes. Additionally, methods are provided utilizing these novel enzymes in catalytic systems for in situ regeneration of NADPH or NADH. Further aspects of the invention relate to the definition and use of crystal structures of these novel enzymes. | 04-08-2010 |
20100124758 | APPARATUS AND METHOD FOR DETECTING GLYCOL - A method and apparatus are provided for detecting contaminants, such as ethylene glycol and diethylene glycol, in various materials, including household products, and medicines. The contaminants can be detected using enzyme assays that produce measurable changes in light absorption and/or light fluorescence. | 05-20-2010 |
20100159495 | PROSECC AN MEANS FOR ENZYMATIC DETERMINATION OF ACETATE - The invention relates to a method and means for the enzymatic determination of acetate. The method according to the invention facilitates for the first time reflectometric detection by means of a test strip. | 06-24-2010 |
20100173343 | METHOD OF DETERMINING 1,5-ANHYDROGLUCITOL, AND REAGENT COMPOSITION FOR DETERMINING 1,5-ANHYDROGLUCITOL - A method for the determination of 1,5-anhydroglucitol, characterized by using any one of the following proteins (a) to (c): (a) a protein which comprises an amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence having the deletion, substitution and/or addition of one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity; and (c) a protein which comprises an amino acid sequence having at least 60% sequence homology to the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity. | 07-08-2010 |
20100227355 | REAGENT COMPOSITIONS FOR USE IN ELECTROCHEMICAL DETECTION - Biological reagent compositions with improved sensitivity to the concentration of blood glucose in patient samples for use in measuring systems and methods. The reagent compositions comprise a glucose oxidoreductase enzyme, a flavin nucleoside coenzyme and a mediator formulation. The mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex. | 09-09-2010 |
20100233747 | METHOD AND MEANS FOR THE ENZYMATIC DETERMINATION OF ETHANOL - The invention relates to a method and means for the enzymatic determination of ethanol. The method according to the invention prevents ethanol vapours in the ambient air from interfering with the determination. | 09-16-2010 |
20100304417 | MARKERS FOR TRANSFORMED EPITHELIUM AND POTENTIAL TARGETS FOR THERAPY OF CANCER OF THE GINGIVO BUCCAL COMPLEX - Systematic comparisons of samples of adjacent clinically non malignant and tumor tissue from cancer of the gingivo buccal complex obtained during surgical resection of the tumor revealed significant differences in protein expression between the cancer tissue and the adjacent clinically non malignant tissue in each patient. This study has identified a set of new proteins which can differentiate between the epithelial tissue from cancer patients which may be useful for high-through put early detection, prognosis, and potential targets for therapy. | 12-02-2010 |
20110039290 | NOVEL DRY STICK DEVICE CONSTRUCTION AND METHOD FOR DETERMINING AN ANALYTE IN A SAMPLE USING SAID DRY STICK DEVICE - A novel dry stick device construction for the determination of an analyte in a sample is provided. The device comprises: (i) optionally a solid support, (ii) at least one reagent pad comprising a reagent or a combination of reagents capable of reacting with the analyte, a derivative of said analyte or an indicator compound for said analyte to provide a detectable signal when in a moistened state, the at least one reagent pad providing a first environment for said reagent(s), said first environment permitting an improved storage stability of the reagent(s) and dry stick device when in a non-moistened state, and (iii) a regulating pad being in contact with the at least one reagent pad, the regulating pad creating a second environment for said reagent(s), when in a moistened state, said second environment permitting an increased rate of reaction between the analyte and the reagent. | 02-17-2011 |
20110091921 | NOVEL 12 ALPHA-HYDROXYSTEROID DEHYDROGENASES, PRODUCTION AND USE THEREOF - The invention relates to 12 α-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 α-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 α-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12α-hydroxysteroids using said 12α-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalysed cholic acid oxidation using said 12 α-hydroxysteroid dehydrogenases. | 04-21-2011 |
20110223629 | METHOD FOR DETERMINATION OF DELTA-D VALUES OF NON- EXCHANGEABLE HYDROGEN STABLE ISOTOPES ON ETHANOL' S METHYL GROUP BY MEANS OF IRMS INSTRUMENTAL TECHNIQUE - The present innovation is related with chemical instrumental analysis, i.e. to the domain of analysis of stable isotopes in food products, and it is related to the method for preparation of ethanol samples and mode for determination of isotopic ratio of non-exchangeable hydrogen stable isotopes sited on the methyl site of ethanol by means of instrumental technique CF-TC/EA-IRMS (Continuous Flow-Temperature Conversion/Elemental Analyzer-Isotope Ratio Mass Spectrometry), and for the purpose of establishing authenticity and geographical origin of wine and grape must, beer, alcoholic beverages, fruit juices, honey, vinegars and other food products which contain alcohol and/or fermentable sugars. This method is based on full enzymatic (or organic) transformation of ethanol samples to gain ethanoic acid (acetic acid), controlled neutralization of acetic acid and further concentration, purification of prepared acetate salt and its drying to the constant mass and further determination of δD values in prepared samples by means of CF-TC/EA-IRMS. | 09-15-2011 |
20110236919 | PROCESS FOR RESTRICTING CARBON MONOXIDE DISSOLUTION IN A SYNGAS FERMENTATION - A process to temporarily and selectively control the dissolved concentration of CO in the feed medium portion of a fermentation liquid by addition of a surface tension active compound (STAC), particularly during seed train scale-up operations and during recovery from process upsets, to support improved production of biofuels from a syngas fermentation. The process inoculates the fermentation liquid when the cell density level is from 0.05 to 0.5 OD with the surface tension active compound at a concentration of from 20 to 800 mg per liter of syngas fermentation liquid. The surface tension active compound may be a polyol or glycol. | 09-29-2011 |
20110236920 | NOVEL VARIANTS OF PQQ-DEPENDENT GLUCOSE DEHYDROGENASE HAVING IMPROVED SUBSTRATE SPECIFICITY - The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof. | 09-29-2011 |
20110256570 | METHOD AND KIT FOR POSITIVELY DETECTING TOXIC MATERIAL - A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample. | 10-20-2011 |
20110262950 | REDOX MEDIATORS - The present invention relates to ruthenium and osmium complexes of Formula [M(A) | 10-27-2011 |
20110306076 | Cellobiose Dehydrogenase - The present invention relates to cellobiose dehydrogenases (CDH) having glucose oxidation activity at a pH of 7.4 or above, modifications to modify the pH dependency of the enzymes activity, uses for these CDHs, in particular electrode sensors and electrochemical cells. | 12-15-2011 |
20120028287 | Crystal Structure of Aldehyde Dehydrogenase and Methods of Use Thereof - The present disclosure provides a crystal structure of aldehyde dehydrogenase (ALDH) with a modulator of ALDH bound thereto. The present disclosure provides a computer readable medium comprising atomic coordinates for an ALDH polypeptide and a modulator bound to a site within the polypeptide. A method is also provided. In general terms, the method comprises computationally identifying a compound that binds to an ALDH polypeptide, using the atomic coordinates. | 02-02-2012 |
20120094320 | THERMOSTABLE 1,5-ANHYDROGLUCITOL DEHYDROGENASE, AND METHOD FOR MEASUREMENT OF 1,5-ANHYDROGLUCITOL BY USING THE SAME - A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability. | 04-19-2012 |
20120094321 | METHOD AND KIT FOR MEASUREMENT OF DEHYDROGENASE OR SUBSTRATE FOR THE DEHYDROGENASE - Disclosed is a method for measuring a dehydrogenase or a substrate for the dehydrogenase contained in a sample, wherein both thio-NAD or thio-NADP and NAD or NADP are used as coenzymes. The method enables the measurement on the basis of absorbance values obtained in a visible region, and also enables the accurate measurement of a dehydrogenase or a substrate for the dehydrogenase which is contained in a sample at a high concentration. | 04-19-2012 |
20120129206 | METHOD OF IDENTIFYING PATIENTS SUITABLE FOR HIGH-DOSE CYCLOPHOSPHAMIDE TREATMENT - The present invention provides methods of identifying subjects that are suitable for high-dose cyclophosphamide treatment. | 05-24-2012 |
20120142041 | Methods And Kits For Measuring Toxicity And Oxidative Stress In Live Cells - Compositions and methods for the measuring toxicity, antioxidant capacity, and oxidative stress are provided. | 06-07-2012 |
20120295295 | DETECTING ISOMERS USING DIFFERENTIAL DERIVATIZATION MASS SPECTROMETRY - Methods of evaluating molecular isomers of branched-chain amino acids are featured. The methods can include: derivatizing one or more molecular isomers of branched-chain amino acids in a sample comprising a branched-chain amino acid labeled with one or more heavy atoms as a first standard; adding, to the sample, after derivatization, a nonderivatized or derivatized branched chain amino acid that is labeled with one or more heavy atoms, as a second standard; evaluating the sample using tandem mass spectrometry; and detecting peaks indicative of derivatized and nonderivatized forms of one or more branched-chain amino acids in the sample. | 11-22-2012 |
20130011871 | STERILIZABLE CHEMISTRY FOR TEST ELEMENTS - In one non-limiting aspect, sterilizable reagent materials for diagnostic elements are provided. In other aspects, sterilized diagnostic elements and techniques for the production of the same are disclosed. In one embodiment, a sterilized diagnostic element includes a chemical detection reagent including at least one component that is sensitive to ionizing radiation. The sterilized diagnostic element is also mediator-free and the at least one component sensitive to ionizing radiation is present in a functional form in a proportion of ≧ 80% based on the total amount of the respective component in the diagnostic element before sterilization. In certain aspects, the at least one component sensitive to ionizing radiation includes one or both of an enzyme and a coenzyme. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving sterilizable reagent materials or sterilized diagnostic elements. | 01-10-2013 |
20130029368 | METABOLIC MATURATION IN STEM CELL-DERIVED TISSUE CELLS - The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example. | 01-31-2013 |
20130052678 | URACIL-SPECIFIC FLUORESCENCE DETECTION REACTION AND METHOD FOR EXAMINING DIHYDROPYRIMIDINE DEHYDROGENASE DEFICIENCY - The invention provides a method of detecting uracil. The method comprises reacting uracil with a compound represented by the formula (I) in the presence of an oxidant and a base to produce a fluorescent compound represented by the formula (II). | 02-28-2013 |
20130071871 | ENZYMATIC DETERMINATION OF LITHIUM IONS USING PHOSPHOGLUCOMUTASE - Kits and methods for measuring lithium ions using phosphoglucomutase are disclosed. | 03-21-2013 |
20130137128 | METHOD OF ASSESSING CHEMICALS IN PRODUCED FLUIDS - A method of monitoring water-soluble treatment chemicals in a fluid that is immiscible with water and which may or may not contain some aqueous fluid, the method using at least one reagent that produces an optically detectable product, the detection step can take place without separation of the aqueous phase containing the treatment chemicals from the fluid immiscible with water. | 05-30-2013 |
20130149730 | EMULSION-DERIVED PARTICLES - An emulsion-derived particle comprises a lattice of polymeric strands cross-linked by means of a cross-linking agent, and interstitial openings adjacent and around the strands. Functional groups are provided on the lattice and proteins and/or modified proteins can react with these, thereby to be bonded to the lattice and hence immobilised. | 06-13-2013 |
20130164771 | MEASURING DEVICE, MEASURING APPARATUS AND METHOD OF MEASURING - The measuring device of the invention includes: a first container and a second container for holding a sample; and an optical measurement part for carrying out an optical measurement. The first container has a first sample supply inlet for supplying a sample containing an analyte to the first container and at least one electrode. The second container has a second sample supply inlet for supplying the sample to the second container and a reagent holding part for holding a reagent for the optical measurement. | 06-27-2013 |
20130171678 | SYSTEM AND METHOD FOR ANALYZING RISK OR PRESENCE OF ORGAN FAILURE - The present invention relates to a testing system for analyzing a risk of developing organ failure or presence of organ failure, said system comprising a disposable testing device arranged to receive a biological sample, which testing system is further arranged with means for measuring within said sample the total amount of at least a first biological marker in the form of an intracellular enzyme, and comprising means for analyzing the measured value of said intracellular enzyme, and based on the results thereof being arranged to communicate the risk of developing organ failure or presence of organ failure to an operator of said testing system. The present invention also relates to a method for analyzing a risk of developing organ failure, or analyzing presence of organ failure. | 07-04-2013 |
20130189721 | Method for Producing Dry Reagent, Dry Reagent, and Analysis Tool Using Same - Provided is a dry reagent that allows measuring with good precision, in accordance with a transmission method that utilizes light of the ultraviolet region, increases or decreases of a nicotinamide coenzyme, in order to quantify a component contained in a liquid sample. | 07-25-2013 |
20130196359 | SYSTEMS AND METHODS FOR BIOTRANSFORMATION OF CARBON DIOXIDE INTO HIGHER CARBON COMPOUNDS - Systems, compounds and methods for the conversion of C1 carbon compounds to higher carbon compounds useful for the generation of commodity compounds. | 08-01-2013 |
20130224781 | DIAGNOSTIC APPARATUS AND DIAGNOSTIC METHOD USING THE SAME - Provided is a diagnostic apparatus. The diagnostic apparatus includes a microfluidic chip including first and second measurement parts for respectively measuring an amount of hemoglobin and an active degree of an enzyme within a blood sample. The second measurement part of the microfluidic chip analyzes the active degree of the enzyme within the blood sample using voltammetry. | 08-29-2013 |
20130244266 | BIOSENSING SYSTEMS FOR MEASUREMENT OF LACTOSE - Herein disclosed are biosensing systems that measure lactose concentration in a solution. | 09-19-2013 |
20130260407 | METHOD FOR THE PURIFICATION AND STABILISATION OF ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3), ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3), AND THE USE OF ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3) - Process of purification and stabilization of the enzyme Gluconate Dehydrogenase (GADH, EC 1.1.99.3) either recombinant or not, from several microorganisms such as: | 10-03-2013 |
20130280745 | HIGH-THROUGHPUT, SENSITIVE DETECTION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE - Provided herein are methods for determining an amount of glucose-6-phosphate dehydrogenase (“G6PDH”) in a biological sample. Also provided herein are methods for detecting G6PDH deficiency, as well as diagnosing G6PDH-associated disorders, such as acute hemolytic anemia and pre-eclampsia. | 10-24-2013 |
20130288284 | METHODS AND COMPOSITIONS FOR CELL-PROLIFERATION-RELATED DISORDERS - Methods of treating and evaluating subjects having neoactive mutants of IDH (e.g., IDH1 or IDH2). | 10-31-2013 |
20130288285 | METHODS FOR PREDICTING CARDIAC TOXICITY - Methods are disclosed for determining whether organ toxicity, particularly cardiotoxicity, will occur in a patient selected for treatment with various kinase inhibitors, such as tyrosine kinase inhibitors, more particularly erbB inhibitors such as Herceptin. In addition, methods are disclosed for determining whether a potential drug is likely to produce a cardiotoxic effect. The methods involve analyzing lipid levels or the expression fatty acid oxidation enzymes, pAMP activated protein kinase, glucose uptake, to determine whether a fatty acid oxidation disorder is present. The identification of a fatty acid oxidation disorder can be used as a predictor of toxicity, especially cardiac toxicity, and as an indication that organ function should be carefully monitored if a drug such as a tyrosine kinase inhibitor is administered. Methods are also disclosed for protecting organs from metabolic stress and for the treatment of cells, such as adipocytes, to reduce their lipid content. | 10-31-2013 |
20130302840 | BLOOD ALCOHOL TEST APPARATUS AND METHOD - The subject matter consists of a small, hand held, portable, battery run device similar to a current diabetic monitor. The device will be carried on a person in a custom protective case that can be attached to a belt, in a pocket or on the waist of a shirt or pants. The monitor test strip will be impregnated with a substance that will be able to detect, with only a small amount of blood (e.g. a drop or a few drops) the BAC of an individual within a relatively short amount of time (e.g. seconds to minutes). | 11-14-2013 |
20130316385 | METHODS AND COMPOSITIONS FOR CELL-PROLIFERATION-RELATED DISORDERS - Methods and compositions for treating and evaluating subjects having a neoactive mutation at residue 97 of IDH1 or 137 of IDH2. | 11-28-2013 |
20130330760 | COMPOSITIONS AND METHODS FOR MONITORING OXALATE - The present invention features compositions and methods for the detection or measurement of oxalate in a sample. Such compositions include test devices that provide for the rapid and accurate detection of oxalate in a sample from a biological fluid. Advantageously, the compositions can be used to monitor the oxalate levels of a patient at a point of care (e.g., at the patient's home, clinic, physician's office, or other clinical setting). | 12-12-2013 |
20130337485 | ENZYMATIC SYNTHESIS OF ACTIVE PHARMACEUTICAL INGREDIENT AND INTERMEDIATES THEREOF - The present invention discloses a process for preparing an active pharmaceutical ingredient (API) or intermediates thereof, notably particular step in the synthesis of an intermediate useful for example in the preparation of statins, by using an enzyme capable of catalyzing oxidation or dehydrogenation. The invention further provides an expression system effectively translating said enzyme. In addition, the invention relates to a specific use of such enzyme for preparing API or intermediate thereof, and in particular for preparing statin or intermediate thereof. | 12-19-2013 |
20140093901 | GENOME-SCALE METABOLIC NETWORK MODEL RECONSTRUCTION OF KLUYVEROMYCES MARXIANUS AND STRATEGIES FOR ENGINEERING NON-NATIVE PATHWAYS FOR 3-HYDROXYPROPIONATE PRODUCTION IN KLUYVEROMYCES MARXIANUS - Use of a metabolic network model for analyzing metabolic characteristics of microorganisms for producing a metabolic product, such as 3HP enabling estimation of productivity and cell growth speed of microorganisms, optimizing new metabolic pathway, and providing transformed microorganisms that may produce a specific metabolic product with high efficiency. | 04-03-2014 |
20140099656 | RAPID DETECTION OF HISTAMINE IN FOOD AND BEVERAGES - Described herein is a rapid, semi-quantitative sampling device which may rapidly detect histamine in food, beverage and feed samples. The device uses an enzyme (impregnated in a sampling pad) to specifically convert histamine present in samples to a visual colorimetric signal. The color change of the pad is proportional to the amount of histamine present in the sample. | 04-10-2014 |
20140106388 | AUTOMATED CTC DETECTION - An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane. | 04-17-2014 |
20140212907 | Methods and Kits for Measuring Toxicity and Oxidative Stress in Live Cells - Compositions and methods for the measuring toxicity, antioxidant capacity, and oxidative stress are provided. | 07-31-2014 |
20140273054 | COUPLED ENZYME-BASED METHOD FOR ELECTRONIC MONITORING OF BIOLOGICAL INDICATOR - A sterilization indicator system and method of using the system to determine efficacy of a sterilization process. The system includes a vial having a first compartment containing spores of one or more species of microorganism; a second compartment containing a growth medium with a disaccharide, an oligosaccharide or a polysaccharide in which the vial is free of monosaccharide; an enzyme, capable of acting upon the monosaccharide to yield reaction products and electron transfer, disposed on two or more electrodes adapted to carry an electrical signal resulting from the electron transfer, the pair of electrodes positioned to contact the combined contents of the first compartment and the second compartment during incubation; and an apparatus linked or linkable to the electrodes and adapted to detect and measure the electrical signal resulting from electron transfer when the enzyme acts upon the monosaccharide. | 09-18-2014 |
20140308695 | DRUG SCREENING AND POTENCY ASSAYS - The present invention concerns bioactive renal cell populations, in particular a B2 cell population comprising an enriched population of tubular cells and wherein the renal cell population is depleted of a B1 cell population, renal cell constructs, and methods of screening test agents using the bioactive renal cell populations. | 10-16-2014 |
20150010936 | Modified Leucine Dehydrogenase - The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme. | 01-08-2015 |
20150044716 | MEANS AND METHODS FOR THE DETERMINATION OF (D)-2-HYDROXYGLUTARATE (D2HG) OR (D)-2-HYDROXYADIPIC ACID - The present invention relates to a method for detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid in a sample, the method comprising the steps of: a) contacting a sample with a reagent mixture, wherein said reagent mixture comprises: (i) a solvent, (ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state, (iii) an electron transfer agent, (iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and (v) a cofactor; and b) detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid by measuring the production of the reduced state of the dye. The invention further pertains to a method for diagnosing and/or monitoring a (D)-2-hydroxy-glutarate-associated disease in a subject. Encompassed by the invention is also a method for diagnosing a mutation in an isocitrate dehydrogenase (IDH) gene or in a (D)-2-hydroxyglutarate (D2HG) dehydrogenase enzyme gene in a subject. In addition, the invention provides for a kit comprising (i) a solvent, (ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state, (iii) an electron transfer agent, (iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and (v) a cofactor. | 02-12-2015 |
20160010141 | HIGH EFFICIENCY METHODS OF PRODUCING BLOOD GLUCOSE TEST ELEMENTS, AS WELL METHODS OF USING THE SAME | 01-14-2016 |
20160040125 | A HUMAN BLOOD-BRAIN BARRIER MODEL DERIVED FROM STEM CELLS - The present disclosure relates to a method for obtaining human brain-like endothelial cells by contacting a population of cells isolated from stem cells with a differentiation medium to obtain endothelial cells and co-culturing said endothelial cells with pericytes, with cells of the neurovascular unit or with a pericytes conditioned medium, to obtain brain-like endothelial cells. The present disclosure also relates to the use of the brain-like endothelial cells as an in vitro model of human blood-brain barrier and a kit for measuring blood-brain barrier permeability of a substance, comprising in vitro human endothelial cells. | 02-11-2016 |
20160061694 | CUSTOMIZED QUALITY CONTROLS FOR ANALYTICAL ASSAYS - Solid beads each containing a selected quantity of analyte are combined and a liquid base matrix that contains attributes of a biological fluid that is to be assayed, together constitute a kit from which a laboratory technician can, at the point of use, prepare a liquid control for a particular analyte, and preferably a series of such controls at different levels of the same analyte customized for a particular assay. | 03-03-2016 |
20160084845 | COMPOSITIONS AND METHODS FOR DETECTING S-NITROSYLATION AND S-SULFINYLATION - The present invention relates to methods for detecting protein S-sulfinylation and S-sulfinylation within thiol groups in proteins, metabolites, or materials. | 03-24-2016 |
20160108391 | REAGENT COMPOSITIONS HAVING PYRIDINE-CARBOXYLIC ACID-STABILIZED ENZYMES, AS WELL AS METHODS OF MAKING AND USING THE SAME - Dry reagent compositions are disclosed that include one or more of an enzyme such as a dehydrogenase, a redox cofactor, an agent capable of eliciting at least one measurable change in a property of an indicator reagent in the presence of redox equivalents, an indicator reagent, and a pyridine-carboxylic acid, derivative or salt thereof. Also provided are diagnostic test elements including the reagent compositions for use in body fluid analysis. Further provided are methods of making test elements, as well as methods of body fluid analysis with such test elements. In the compositions, the pyridine-carboxylic acid, derivative or salt thereof attenuates, reduces or prevents a decrease of enzymatic activity of the at least one dehydrogenase under dry and/or humid conditions. | 04-21-2016 |
20160168613 | DEVICE AND METHODS OF USING DEVICE FOR DETECTION OF AMINOACIDOPATHIES | 06-16-2016 |
20160376631 | MATERIALS AND METHODS FOR RAPID VISUALIZATION OF NAD(P)H - The subject invention provides materials and methods for detecting the presence of an electron donor. In a specific embodiment, the device detects the presence of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric change output by the sensing device. In another specific embodiment, the presence of an enzyme capable of catalyzing, or an agent capable of inhibiting, the production of NADH can also be detected by a colorimetric readout using the same device. In some embodiments, dihydronicotinamide adenine dinucleotide phosphate (NADPH) can also be detected using the device provided herein. Advantageously, preferred embodiments of the subject invention provide a low-cost, sensitive device for monitoring the presence of critical biological analytes in a variety of applications. | 12-29-2016 |
20180023113 | Acyl-CoA Dehydrogenases Micro/Nano Enzyme Assay | 01-25-2018 |