Entries |
Document | Title | Date |
20080213813 | Histone deacetylase inhibitor and use thereof - To provide a novel apoptosis inducer, and a method of screening an apoptosis inducer. For example, an apoptosis inducer comprising inhibiting HDAC6 such as an antisense oligonucleotide to the gene of histone deacetylase 6 (HDAC6), and anti-cancer agent comprising this apoptosis inducer, and the present invention also provides a method of screening an apoptosis inducer that inhibits HDAC6, and more specifically a method of screening an apoptosis inducer said method comprising the steps of (1) determining whether or not a test substance inhibits histone deacetylase 6 (HDAC6) by using deacetylation of an acetylated substance that can be a substrate for histone deacetylase 6 (HDAC6) or decrease in the expression of histone deacetylase 6 (HDAC6) as an index; and (2) confirming, when inhibition is present, whether it induces apoptosis of the cell in vitro and/or in vivo. | 09-04-2008 |
20080280315 | Swatch for Testing the Washing Performance of an Enzyme - The present invention relates to a swatch comprising a pH-indicator substance and a substrate for an enzyme for testing the washing performance of the enzyme, e.g. an enzyme for use in detergent compositions. | 11-13-2008 |
20090011450 | Dry analytical element for lipase measurement - It is an object of the present invention to provide a dry analytical element for pancreatic lipase analysis having high selectivity with respect to pancreatic lipase, whose multianalyte correlation has been improved. The present invention provides a dry analytical element for measurement of pancreatic lipase contained in a body fluid, which comprises at least one development layer and/or reagent layer containing diglyceride or triglyceride of long-chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, wherein the development layer and/or the reagent layer comprise two or more types of anionic surfactants and at least one type of the anionic surfactant is alkylarylsulfonate. | 01-08-2009 |
20090017481 | Method For Identifying at Least Two Groups of Microorganisms - The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps:
| 01-15-2009 |
20090061470 | Use of Human Hepatocytes for Determining Liver Function and Liver Regeneration - The use of human hepatocytes to determine the liver function and the liver regeneration in a test person, in particular a patient, as well as the use of these human hepatocytes as parameters for prognoses. Also, an in-vitro method to determine the liver function of a test person, in particular a patient using human hepatocytes. | 03-05-2009 |
20090068696 | COMPOSITIONS AND MEANS FOR DIAGNOSING MICROBIAL INFECTIONS - The present invention pertains to the need for novel, reliable, fast and inexpensive approaches to diagnosing, including detecting and characterising microbial infections in humans and animals or methods for detecting and characterising microbial infections in various environments, such as in a food or feed sample. | 03-12-2009 |
20090068697 | Compounds and methods of use thereof for assaying lysophospholipase D activity - Fluorogenic lysophosphatidic acid derivatives which can be used as substrates in a continuous, fluorogenic assay that can be performed in microtiter plates. The assays permit measuring LysoPLD activity levels in normal events such as pregnancy or disease states such as cancer. In addition, the present invention can be adopted to high throughout screening (HTS) for identification of potential inhibitors of lysoPLD activity. | 03-12-2009 |
20090075314 | SINGLE-CELL BIOSENSOR FOR THE MEASUREMENT OF GPCR LIGANDS IN A TEST SAMPLE - The present invention is related to the detection of GPCR ligands in a test sample by using a single cell biosensor expressing a GPCR. Preferably, the test sample is derived from a biological or environmental sample. This invention may be used to detect the presence of a disease or to detect the presence of a harmful agent in the environment. Included in the present invention is an array of biosensors that detect ligands of various GPCRs. | 03-19-2009 |
20090087871 | Method for Identifying PDE5-Modulators - The invention relates to a novel polypeptide containing the GAF | 04-02-2009 |
20090093008 | METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM - According to the following steps, live cells, injured cells, VNC cells and dead cells of a microorganism in a test sample are detected by flow cytometry: | 04-09-2009 |
20090117602 | Plasmodium Diagnostic Assay Device - This invention relates to assays for a | 05-07-2009 |
20090117603 | STERILIZATION INDICATOR - The disclosed technology relates to a sterilization indicator and a process to concentrate signal generated by constraining it to a minimal surface, in a minimal volume and minimal pH and growth buffering or mediating influences. The sterilization indicator may comprise a carrier | 05-07-2009 |
20090136979 | Class IIA Histone Deacetylase (HDAC) Substrate - The present invention relates to the use of trifluoroacetylated lysine (Boc-L-Lys(ε- trifluoroacetyl)-MCA) as a substrate for histone deacetylases, specific for the class Ha subtype. | 05-28-2009 |
20090142786 | SECRETED AND TRANSMEMBRANE POLYPEPTIDES AND NUCLEIC ACIDS ENCODING THE SAME - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. | 06-04-2009 |
20090170146 | Novel Carboxylesterase Nucleic Acid Molecules, Proteins and Uses Thereof - The present invention relates to arthropod esterase proteins; to arthropod esterase nucleic acid molecules, including those that encode such esterase proteins; to antibodies raised against such esterase proteins; and to other compounds that inhibit arthropod esterase activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to protect animals from hematophagous arthropod infestation. | 07-02-2009 |
20090181416 | Enzyme Detection Technique - A diagnostic test kit for detecting the presence or absence of an enzyme (e.g., leukocyte esterase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the enzyme to release a nucleophilic aromatic compound. The kit also comprises a lateral flow device that comprises a chromatographic medium. The chromatographic medium defines a detection zone within which is contained a first reagent (e.g., diazonium ion) that is capable of reacting with the nucleophilic aromatic compound to form a second reagent (e.g., aromatic azo compound). The second reagent exhibits a color that is different than the color of the first reagent. The lateral flow device also includes an absorbent material located adjacent to the chromatographic medium, the absorbent material receiving the test sample after flowing through the chromatographic medium. | 07-16-2009 |
20090208992 | PARAOXONASE 1 ENZYMATIC ACTIVITY, A RISK INDICATOR FOR MAJOR ADVERSE CARDIOVASCULAR EVENTS - Provided herein are methods for assessing the risk a subject or patient without significant evidence of cardiovascular disease has of experiencing a major adverse cardiac event or requiring revascularization near term. Also provided are methods of determining whether a subject who has experienced a major adverse cardiac event is at risk of experiencing a recurrent major adverse cardiac event near term. The present methods comprise determining the levels of paraoxonase 1 enzymatic activity in the blood, serum, plasma, or any combination of said bodily fluids in the subject. | 08-20-2009 |
20090246811 | DRY ANALYTICAL ELEMENT FOR LIPASE MEASUREMENT - The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 μm or less. | 10-01-2009 |
20090246812 | DRY ANALYTICAL ELEMENT FOR LIPASE MEASUREMENT - It is an object of the present invention to provide: a dry analytical element for analyzing pancreatic lipase wherein the triglyceride is not transcribed on the support to contaminate a transportation slip or other analytical elements and wherein an additive solution of the triglyceride neither reaggregates nor precipitate, so that the dry analytical element is stable and is compatible with production. The present invention provides a dry analytical element for measuring pancreatic lipase contained in body fluid, which comprises triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerin measurement reagent, and which comprises a water-impermeable support and at least one spreading or reagent layer, wherein a hydrophilic polymer at a weight ratio of 1.8:1 or greater with respect to the triglyceride is contained. | 10-01-2009 |
20090258380 | Perhydrolase Epitopes - The present invention provides perhydrolase enzyme CD4+ T-cell epitopes, as well as variants that exhibit reduced immunogenic responses, as compared to the parental perhydrolase . The present invention further provides DNA molecules that encode perhydrolase variants, and host cells comprising DNA encoding perhydrolase variants, as well as methods for making perhydrolase enzymes less immunogenic. In addition, the present invention provides various compositions that comprise perhydrolase variants that are less immunogenic than the wild-type perhydrolase. In some specific embodiments, the present invention provides perhydrolase variants with reduced immunogenicity identified and/or characterized using the methods of the present invention. These enzymes find use in cleaning and other applications. In some preferred embodiments, the present invention finds particular use in applications involving cleaning, bleaching and disinfecting. | 10-15-2009 |
20090263844 | METHOD AND KIT FOR QUANTITATIVE DETERMINATION FOR SMALL, DENSE PARTICLE LOW DENSITY LIPOPROTEINS - A rapid and convenient method capable of performing fractional measurement of small, dense LDLs without pretreatment of a specimen, which is adaptable for an autoanalyzer, is provided. A method for quantitatively determining small, dense LDL cholesterol is provided, which comprises adding enzymes for cholesterol measurement to a test sample in the presence of a polyoxyethylene-polyoxypropylene copolymer or a derivative thereof, causing the polyoxyethylene-polyoxypropylene copolymer or the derivative thereof to selectively act on small, dense LDLs among lipoproteins, and then measuring the amount of cholesterol generated. | 10-22-2009 |
20090298108 | Method for Identifying PDE11 Modulators - The invention relates to a novel GAF | 12-03-2009 |
20100028923 | Cross-reactive sensors - The present invention provides a novel cross-reactive sensor system utilizing cross-reactive recognition elements. In the inventive system, each of said one or more cross-reactive recognition elements is capable of interacting with more than one species of liquid analyte of interest, whereby each of said one or more cross-reactive recognition elements reacts in a different manner with each of said one or more species of liquid analytes of interest to produce a detectable agent of each analyte of interest, whereby said detectable agent is analyzed and the information is processed for data acquisition and interpretation. In certain preferred embodiments, the detectable agent and/or change is detected directly, while in certain other preferred embodiments, the detectable agent and/or change is detected with the help of a transducing agent capable of relaying information about each detectable agent generated for each of said species of liquid analyte of interest, whereby said information is processed for data acquisition and interpretation. The present invention also provides method for the analysis of analytes comprising contacting one or more analytes with the inventive system described above. | 02-04-2010 |
20100028924 | Method Of Diagnosing Pon1-Hdl Associated Lipid Disorders - Methods and kits for diagnosing a lipid-related disorder are disclosed. Methods and pharmaceutical compositions for treating lipid-related disorders are also disclosed. | 02-04-2010 |
20100047836 | LIPOLYTIC ENZYME VARIANTS - Molecular dynamics (MD) simulation on the three-dimensional structure of | 02-25-2010 |
20100093009 | ALTERNATIVE CRYSTAL FORM OF MONOACYLGLYCEROL LIPASE (MGLL) - A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized forms of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed. The engineered constructs of MGLL permit the expression and purification of protein suitable for crystallography or high-throughput screening and identification of ligands, which can function as active agents to MGLL. The X-ray diffraction patterns allow the three dimensional structure of MGLL to be determined at atomic resolution so that ligand binding sites on MGLL can be identified and the interactions of ligands with MGLL amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MGLL. | 04-15-2010 |
20100184108 | METHODS AND COMPOSITIONS RELATING TO ENZYME ASSAYS - Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent. | 07-22-2010 |
20100209953 | METHOD OF DETERMINING CARBONIC ANHYDRASE I ACTIVITY - A method for determining hydrolase activity of carbonic anhydrase I (CAI) in a sample which employs, combination of a substrate and an inhibitor. The substrate is a substrate having higher reactivity with CAI than with CAII selected from 2-hydroxy-5-nitro-α-toluenesulfonic acid sultone, a o-nitrophenyl ester, a p-nitropheylthio ester, and a β-naphthyl ester or a substrate having reactivity with both CAI and CAII selected from the group consisting of a p-nitrophenyl ester and a α-naphthyl ester. The substrate having higher reactivity with CAI than with CAII is a substrate that reacts with CAI in an amount, per amount of enzyme protein, twice or more the amount of substrate reacting with CAII, under identical substrate concentrations and reaction times and that specifically binds to CAI and serves as a substrate for hydrolase activity. The inhibitor is an inhibitor inhibiting a hydrolase other than CA, a CA inhibitor inhibiting both CAI and CAII, or a CA inhibitor inhibiting CAI more potently than CAII. | 08-19-2010 |
20100248281 | RAPID DETECTION OF REPLICATING CELLS - The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance. | 09-30-2010 |
20100273196 | System and Method for Evaluating Blood-Neural Barrier Permeability - A system and method for evaluating blood-neural barrier permeability. Phospholipid liposomes are labeled with a fluorescent phospholipase A | 10-28-2010 |
20110039287 | METHOD FOR QUANTIFICATION OF PEPTIDE AND PROTEIN - A determination method quantifies a specific protein or peptide contained in a trace amount with high accuracy and in a simple manner without the need of using any expensive reagent. A protein or peptide of interest can be quantified by LC/MS/MS by using, as an internal standard, a protein or peptide including an amino acid sequence having the reshuffling the binding order of amino acid residues in the amino acid sequence for the protein or peptide of interest. | 02-17-2011 |
20110070601 | CLASSIFICATION OF INDIVIDUALS SUFFERING FROM CARDIOVASCULAR DISEASES ACCORDING TO SURVIVAL PROGNOSES AS FOUND BY MEASURING THE LEVELS OF BIOMARKER YKL-40 - The present invention relates to the method of measuring the YKL-40 level and using this measurement as a prognosis for survival of an individual suffering from heart disease caused by atherosclerosis. The method may be used for classification of individuals in order to optimize treatment or monitoring the individuals during the course of or prior to or after treatment. The individual may suffer from any type of cardiovascular disease or disorder. The method also detects and determines whether diagnostically or prognostically significant levels of YKL-40 molecules are present in a biological sample. Furthermore the level of YKL-40 may be used to predict disease relapse. | 03-24-2011 |
20110086374 | Novel Cell-Based Phosphodiesterase Assays - The present invention relates to improved cell-based assays for the in vivo assessment of phosphodiesterase (PDE) activity using cyclic nucleotide-gated channels as cyclic nucleotide sensors, and for the assessment of the effect of PDE modulating compounds. | 04-14-2011 |
20110097756 | APEX AS A MARKER FOR LUNG CANCER - The present invention relates to the assessment of lung cancer. It discloses the use of protein AP endonuclease (APEX) in the assessment of lung cancer. It also relates to a method for assessing lung cancer in vitro using a liquid sample derived from an individual by measuring APEX in the sample. Measurement of APEX can, e.g., be used in the early detection or in the follow-up of patients with lung cancer. | 04-28-2011 |
20110136159 | METHOD AND APPARATUS FOR VIABLE AND NONVIABLE PROKARYOTIC AND EUKARYOTIC CELL QUANTITATION - A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed. | 06-09-2011 |
20110165606 | METHODS FOR MODIFYING, ISOLATING, DETECTING, VISUALIZING, AND QUANTIFYING CITRULLINATED AND/OR HOMOCITRULLINATED PEPTIDES, POLYPEPTIDES AND PROTEINS - The present invention relates to methods and compositions for modifying, isolating, detecting, visualizing, and quantifying citrulline and/or homocitrulline-containing peptides, polypeptides, and proteins using mono- and disubstituted glyoxal derivatives. The invention also provides kits for modifying, isolating, detecting, visualizing, and quantifying the relative amounts of citrulline and/or homocitrulline-containing peptides, polypeptides, or proteins in solutions or samples. | 07-07-2011 |
20110195440 | SYSTEMS AND METHODS FOR IDENTIFYING ALLERGIES - Systems and methods for identifying allergies. In at least one embodiment of a system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for a non-food allergy in a body fluid comprising the diagnostic marker and a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
20110207158 | Method for the non-invasive detection of microorganisms in a closed container - The present invention relates to a method for detecting a contamination with a microorganism in a closed container, in which an extracellular enzyme of the microorganism is detected. The method can very suitably be carried out by providing a substrate for this enzyme to the contents of the container and detecting the conversion of this substrate by this enzyme. An embodiment of an apparatus according to the invention is shown in, which shows a container ( | 08-25-2011 |
20110207159 | SCREENING METHOD FOR EFFICIENTLY OBTAINING USEFUL MICROBIAL STRAINS FROM MICROBIAL SAMPLE OBTAINED FROM NATURAL ENVIRONMENT AND REAGENT AND SCREENING KIT TO BE USED THEREFOR - A screening method for efficiently obtaining useful microbial strains from a sample comprises a first step of inoculating a culture medium with the sample potentially containing multiple microbial cells to culture the sample in the presence of a cell enlarger; a second step of introducing an esterase substrate fluorescent stain into the culture medium; a third step of isolating enlarged microbial cells stained in the second step according to a micromanipulation method to produce multiple culture media for establishment each inoculated with a single microbial cell; a fourth step of culturing the microbial cell in each of the culture media for establishment; and a fifth step of selecting culture media for establishment, in which cell proliferation has been confirmed, among the multiple culture media for establishment cultured in the fourth step. | 08-25-2011 |
20110207160 | METHOD FOR MEASURING LOW DENSITY LIPOPROTEIN CHOLESTEROL - It is an object of the present invention to provide a method capable of measuring low density lipoprotein cholesterol (LDL) in a body fluid with high selectivity. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises treating the body fluid with a polymer compound having an allylamine or diallylamine unit, and measuring the low density lipoprotein cholesterol (LDL-C) using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase. | 08-25-2011 |
20110212477 | LIPOPROTEIN-ASSOCIATED MARKERS FOR CARDIOVASCULAR DISEASE - The invention provides methods of screening a mammalian subject to determine if the subject is at risk to develop, or is suffering from, cardiovascular disease. The methods comprise detecting an amount of at least one biomarker in a biological sample, or HDL subfraction thereof, from the subject, and comparing the detected amount of the biomarker to a predetermined value, where a difference between the detected amount and the predetermined value is indicative of the presence or risk of cardiovascular disease in the subject. In some embodiments, the biomarker comprises at least one of ApoC-IV, Paraoxonase 1, C3, C4, ApoA-IV, ApoE, ApoL1, C4B1, Histone H2A, ApoC-II, ApoM, Vitronectin, Haptoglobin-related protein, and Clusterin, or combinations thereof. | 09-01-2011 |
20110229919 | COMBINATION OF SPLA2 ACTIVITY AND OXPL/APOB CARDIOVASCULAR RISK FACTORS FOR THE DIAGNOSIS/PROGNOSIS OF A CARDIOVASCULAR DISEASE/EVENT. - A method of identifying a subject having or at risk of having or developing a cardiovascular disease and/or a cardiovascular event, includes:
| 09-22-2011 |
20110256566 | Determination of biological material ingredients - The invention relates to a method for analysing ingredients, in particular lipids and/or vitamins and biological materials with a concentration of the biological material ingredients, to uses of relevant organic solvents or organic solvent mixtures, and to a spectrophotometer for measuring biological material ingredients. It is proposed to treat the biological materials with at least one organic solvent which extracts the ingredients, to convert the biological materials to a solidified form during the extraction, with the result that a solidified sediment and a liquid organic phase as the supernatant are formed, and to examine the extracted ingredients in the supernatant. | 10-20-2011 |
20110312008 | Enzymatic Assay for the Quantitative Determination of Phospholipase A1 or A2 Activity in a Sample - The invention relates to a new method for measuring phospholipase A1 or A2 activity in a sample, using a solid phase coated with a fluorochrome-labelled phospholipase A1 or A2 substrate, wherein the molecular coverage is in the range from 8 to 30 fluorochrome-labelled phospholipase A1 or A2 substrate molecules/nm2, and kit for carrying out said method. | 12-22-2011 |
20120009613 | PHARMACEUTICAL COMPOSITION FOR MODULATING THE ACTIVITY OF A NOVEL TRIGLYCERIDE HYDROLASE - Use of an inhibitor or activator of the triglyceride hydrolyse activity of a protein comprising a polypeptide strand encoded by the DNA sequence according to SEQ No. 1 for the preparation of a pharmaceutical composition for the treatment of medical disorders where it is desirable to modulate the activity of a protein encoded by the DNA sequence according to SEQ No. 1. | 01-12-2012 |
20120034637 | DETECTION OF TRICHOMONAS AND CANDIDA - The invention relates to a method and a kit for the detection of | 02-09-2012 |
20120142038 | KITS FOR ASSAYING ENZYME-MEDIATED OXIDATIVE DEMETHYLATION - Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent. | 06-07-2012 |
20120171709 | Method for Identifying Bacteria from the Bacillus Cereus Group - The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: | 07-05-2012 |
20120178117 | METHOD OF LIPID ASSAY AND REAGENT FOR USE THEREIN - A method of lipid assay characterized by assaying the lipids contained in a blood component in the presence of an organic silicon compound. The method can cause specific conditions for direct methods while satisfying requirements such as no influence on precision of assay, no burden on assay apparatus, and easy availability. | 07-12-2012 |
20120190054 | Method of Analyzing Cellulose Decay in Cellulosic Material Hydrolysis - Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed. | 07-26-2012 |
20120208221 | MANUFACTURE OF ACTIVE HIGHLY PHOSPHORYLATED HUMAN LYSOSOMAL SULFATASE ENZYMES AND USES THEREOF - This invention provides compositions of active highly phosphorylated lysosomal sulfatase enzymes, their pharmaceutical compositions, methods of producing and purifying such lysosomal sulfatase enzymes and compositions and their use in the diagnosis, prophylaxis, or treatment of diseases and conditions, including particularly lysosomal storage diseases that are caused by, or associated with, a deficiency in the lysosomal sulfatase enzyme. | 08-16-2012 |
20120219979 | PON1 AS A MARKER FOR HEART FAILURE - Provided herein are methods for assessing the risk a test subject with heart failure has of experiencing a major adverse cardiac event, requiring revascularization, requiring a heart transplant, requiring unscheduled hospitalization for heart failure, progression of heart failure status, or any combination thereof. Also provided herein are methods for assessing the risk a test subject has of developing heart failure. The present methods comprise determining the levels of paraoxonase 1 activity in the serum, non-chelated plasma, or both in the test subject and comparing the level of PON1 activity in the test subject's sample with a control or baseline value based on levels of PON1 activity in serum, non-chelated plasma, or both samples from a population of control subjects. Also provided herein are kits useful in assessing such risks. | 08-30-2012 |
20120264153 | METHOD OF DETECTING PHYTASE ACTIVITY OR PROTEASE ACTIVITY - A method of detecting a phytase activity or a protease activity is described. The method comprises the steps of: (a) providing a composition comprising a phytate/protein complex in a liquid or a gel; wherein the phytate/protein complex provides a detectable property to the composition; (b) providing a sample that comprises or is suspected of comprising phytase activity and/or protease activity, wherein the phytase and/or protease activity is capable of causing a change in the detectable property of the composition; (c) contacting the composition with the sample; and (d) determining if there is a detectable change in detectable property of the composition. | 10-18-2012 |
20120276569 | METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY - This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor. | 11-01-2012 |
20120301910 | DETECTION OF LYSOPHOSPHATIDYLCHOLINE FOR PROGNOSIS OR DIAGNOSIS OF A SYSTEMIC INFLAMMATORY CONDITION - The present invention provides methods and compositions useful for the diagnosis or prognosis of a systemic inflammatory condition such as sepsis. | 11-29-2012 |
20120309036 | Test Arrangement - The invention relates to an arrangement, comprising a solid carrier and a matrix arranged on the solid carrier, said matrix comprising at least one enzymatically convertible or modifiable molecule and comprising at least one enzyme that can be released by the conversion or modification of the molecule, said enzyme being capable of converting at least one color-changing substrate located in the matrix and/or on the solid carrier. | 12-06-2012 |
20130034873 | METHOD FOR THE EXTRACTION AND DETECTION OF FAT-SOLUBLE COMPONENTS FROM BIOLOGICAL MATERIALS - The present invention relates to a method for the analysis of fat-soluble components, in particular dyes, from biological materials, in particular lipid rich foodstuffs, having an enrichment of the components and subsequent analysis. The method comprises a combination of extraction and separation steps and a subsequent analysis step. The invention further relates to an analytical kit and analytical equipment for carrying out the method. The method according to the invention is composed of a plurality of steps. The critical steps which are essential and characterize the invention are: 1. Pre-treatment of the sample to remove lipids. 2. Extracting the dyes into an extraction mixture by a specific solvent or solvent mixture. 3. Destruction of the oxidative sensible ingredients such as carotenoids prior to the final detection and quantification by eye or by optical enhancement. | 02-07-2013 |
20130040328 | ASSAY FOR PHYTOL-FREE CHLOROPHYLL DERIVATIVES - The present invention provides a method for detecting a phytol-free chlorophyll derivative in a sample, comprising a step of detecting a fluorescent signal associated with the phytol-free chlorophyll derivative, wherein a fluorescent signal associated with chlorophyll or a phytol-containing chlorophyll derivative in the sample is quenched. The method may be used for quantifying activity of chlorophyllases and related enzymes in a sample without solvent fractionation of substrate and product. | 02-14-2013 |
20130059320 | Selective growth media for campylobacter bacteria and plating media with said growth media - A selective growth media for | 03-07-2013 |
20130065264 | METHOD AND MEDIUM FOR DETECTING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI BACTERIA - The present invention relates to a method for specific and direct detection of Shiga toxin-producing | 03-14-2013 |
20130089883 | QUICK METHOD FOR DETECTING ENYZMES AND MICROORANISMS - The present invention concerns an in vitro method for detecting an enzyme or a microorganism in a biological sample, comprising the steps of:
| 04-11-2013 |
20130130293 | Method for Reducing Star Activity in Restriction Endonucleases - Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated. | 05-23-2013 |
20130143250 | CYCLIC NUCLEOTIDE-SPECIFIC PHOSPHODIESTERASES FROM LEISHMANIA AND USES THEREOF - The present invention relates to novel amino acid and nucleic acid sequences of yclic nucleotide-specific phosphodiesterases from the parasite | 06-06-2013 |
20130183701 | Fluorogenic Sensors for Phospholipase C Isozymes - The present invention provides fluorogenic substrates and methods of use in detecting and analyzing phospholipase C isozyme (PLC) activity. | 07-18-2013 |
20130224778 | Methods for Determination of Ethanol Consumption - The present invention discloses a method for assessment of previous ethanol exposure comprising the steps of: (i) obtaining a sample from the body of a subject; (ii) quantitatively determining the level of one or several bio-precursors of PEth (Formula I) and the level of the corresponding one or several PEth-homologues (Formula II) in the sample; and (iii) obtaining a ratio between the level of one or several bio-precursors of PEth and the level of the corresponding one or several PEth-homologues; wherein the subject is a human or animal. Additional related methods are also disclosed. | 08-29-2013 |
20130230876 | METHODS AND COMPOSITIONS TO DETECT A BIOLOGICAL ACTIVITY - Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, β-naphthylamine, β-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided. | 09-05-2013 |
20130236920 | POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA - The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. | 09-12-2013 |
20130236921 | Manufacture of Active Highly Phosphorylated Human Lysosomal Sulfatase Enzymes and Uses Thereof - This invention provides compositions of active highly phosphorylated lysosomal sulfatase enzymes, their pharmaceutical compositions, methods of producing and purifying such lysosomal sulfatase enzymes and compositions and their use in the diagnosis, prophylaxis, or treatment of diseases and conditions, including particularly lysosomal storage diseases that are caused by, or associated with, a deficiency in the lysosomal sulfatase enzyme. | 09-12-2013 |
20130252268 | Enzyme substrates for visualizing acidic organelles - The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues. | 09-26-2013 |
20130330756 | DETECTION OF BACTERIA EXHIBITING ENZYMATIC RESISTANCE TO CARBAPENEMS - A process for detecting and/or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems by producing carbapenemase, includes: a) contacting said sample with a reaction medium including at least one carbapenem-class antibiotic, and cloxacillin; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting enzymatic resistance to carbapenems. Advantageously, the medium employed in step a) also contains phenylalanine-arginine-beta-naphthylamide (PAbetaN). | 12-12-2013 |
20130337483 | SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT - Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample. | 12-19-2013 |
20130344522 | DETECTION OF BACTERIA EXHIBITING A RESISTANCE TO CARBAPENEMS - Disclosed is a process for detecting and/or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems, including: a) contacting said sample with a reaction medium including at least one chromogenic agent and faropenem and/or doripenem; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting a resistance to carbapenems. The medium employed in step a) also contains cloxacillin and/or a combination of cloxacillin and PAbetaN. | 12-26-2013 |
20140087407 | Methods of Measuring Protein and/or Fat Digestibility and Uses Thereof - The present invention provides methods for continuous measurement of protein and/or triglyceride digestibility during a meal. Stable isotope of | 03-27-2014 |
20140093896 | Point-Of-Care, Medical Condition Screening Kit - A point-of-care, screening kit for use by a heath care worker to create custom test strips for screening the bodily fluids of an individual for various, medical conditions includes: (a) a plurality of reagents ( | 04-03-2014 |
20140127732 | INDICATOR DEVICE - The present invention provides a test device for indicating the presence, concentration and effectiveness of chemical and biological agents. The test device interacts with the target agent producing a detectable signal indicating the presence, concentration and effectiveness of the agent. The test device includes a first carrier which changes color consequent to interaction with the target agent in proportion to the concentration of the target agent. The test device includes a second carrier which remains unreacted and unchanged in color in the presence of the target agent. The second carrier serves as an integrated reference to which the color change of the first carrier is compared. | 05-08-2014 |
20140162300 | SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT - Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide. | 06-12-2014 |
20140170689 | Modified Diguanylate Cyclase-Phosphodiesterase and Method for Enzymatic Production of Cyclic-diGMP - The present invention provides a recombinant polypeptide comprising a first portion and a second portion, wherein the sequence of the first portion is fully identical to amino acids 1 to 248 of the sequence set forth as SEQ ID NO:1 and the sequence of the second portion is other than amino acids 249 to 511 of the sequence set forth as SEQ ID NO:1. | 06-19-2014 |
20140178914 | FLUORESCENT AND COLORED PROTEINS AND METHODS FOR USING THEM - The field of this invention relates to methods for combining genetic elements such that the activity of one of the elements provides a means for identifying, enriching, selecting for, or enhancing the activity of a second element. The invention also includes specific elements and combinations of elements. | 06-26-2014 |
20140186868 | METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME - The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme. | 07-03-2014 |
20140255963 | Degradable Detergents - Methods and materials relate to degradable detergents. The degradable detergents have degradable linkages that are cleaved when subjected to elevated temperature and/or reduced pressure. The detergents are compatible with spectrometric analysis, such as mass spectrometry. The surfactant comprises at least one fluorinated alkyl moiety and at least one cleavable moiety, wherein the surfactant degrades into a plurality of volatile degradation products when injected into a mass spectrometer. | 09-11-2014 |
20140295474 | METHOD FOR DISSOLUTION TESTING OF SOLID COMPOSITIONS CONTAINING DIGESTIVE ENZYMES - The invention is directed to a process for measuring the amount of digestive enzymes released from a solid composition in a dissolution medium by fluorescence spectroscopy. The invention is also directed to a combined method for measuring both the dissolution and gastroresistance of a solid compositions comprising pancrelipase. | 10-02-2014 |
20140342387 | ENZYMES FOR DEGRADING ORGANOPHOSPHATES - The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from | 11-20-2014 |
20140349326 | APPARATUS AND METHODS FOR ANALYZING A MEDICAL CONDITION - Apparatus and methods are provided for analyzing a medical condition of a user. The apparatus may include a user interface configured to receive user identification information inputted by the user, an analyzer, and a processor all disposed within a common housing. The analyzer is configured to receive a biological specimen from the user and to analyze the biological specimen to generate analysis information. The processor is configured to store and forward the analysis information and to receive prescription information. The apparatus may include a communication unit configured to transmit the user identification information and the analysis information to a doctor at a remote location for review and to receive the prescription information from the doctor. The apparatus then may dispense the prescribed medication or print a medication prescription. | 11-27-2014 |
20150010934 | METHODS FOR DETECTING RISK OF FATAL PROSTATE CANCER USING SERUM CALCIUM - A method of screening for increased risk of fatal prostate cancer in a subject comprises providing a blood sample collected from the subject, and then detecting the presence or absence of an increased level of serum calcium in the sample. An increased level of serum calcium indicates the subject is at increased risk of fatal prostate cancer. | 01-08-2015 |
20150017671 | METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY - This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor. | 01-15-2015 |
20150044714 | sPLA2 MONITORING STRIP - A device and method for determining the presence or absence, or the level of, sPLA2 activity in a fluid sample. The device includes an absorbent matrix that defines a flow path for a fluid sample, a first region of the absorbent matrix for applying a fluid sample, where one of the components selected from a bioactive sPLA2 substrate and a label is dried onto or within the first region of the absorbent matrix, a second region of the absorbent matrix downstream of, and in fluid communication with, the first region for detecting an aggregated reaction product, where the other component not present in the first region is dried onto or within the second region of the absorbent matrix. | 02-12-2015 |
20150044715 | METHOD FOR ANALYZING FORMYL GLYCINE RESIDUE - Disclosed is a method which enables semiquantitative or quantitative determination of the ratio between cysteine and formylglycine residues in a protein. The method includes (a) a step of labeling the protein (i) with a halogen-substituted carboxylic acid, (ii) with a halogen-substituted carboxylic acid amide, and (iii) with a halogen-substituted carboxylic acid and then with hydrazine, or with a halogen-substituted carboxylic acid and then by oximation, (b) a step of digesting each labeled protein to provide a corresponding mixture of peptide fragments, (c) a step of subjecting each mixture to reverse phase chromatography to separate the peptide fragments from each other to produce a chromatogram, (d) a step of comparing the produced chromatograms with each other to identify the peak corresponding to the peptide fragment that contained a cysteine residue and the peak corresponding to the peptide fragment that contained a formylglycine residue. | 02-12-2015 |
20150050680 | Measuring Method of Enzyme Activity - Providing is a new enzyme assay method for enzymes having a water-insoluble or substantially water-insoluble substrate. In the method for measuring enzymatic activity, a prescribed amount of an enzyme is disposed on a part of the surface of a gel comprising dispersoids, at least some of which are the substrate of the enzyme. Recesses formed in the surface of the gel by the action of the enzyme are measured, and the enzyme activity is calculated on the basis of the measurement results and the amount of the enzyme. The measurement of the recesses formed in the surface of the gel is performed using a method for measuring the shape and the volume of the recesses, a method for measuring changes in the optical transmittance of the gel due to the formation of the recesses, or a method for measuring changes in the optical reflectance of the gel surface due to the formation of the recesses. | 02-19-2015 |
20150072366 | Methods for Measuring Fat Digestibility and Uses Thereof - The present invention provides methods for continuous measurement of triglyceride digestibility during a meal. A stable isotope labeled triglyceride and a free fatty acid tracer are added to the meal. The ratio between a ratio of isotope labeled fatty acid produced after digestion to free fatty acid tracer represents the percentage of digestion of triglycerides by lipase from the pancreas. | 03-12-2015 |
20150140588 | Lipase Variants and Polynucleotides Encoding Same - The present invention relates to variants with improved activity in an amide-bond reaction. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 05-21-2015 |
20150291998 | METHOD FOR EVALUATION OF DRUG EFFICACY OF A MEDICINE HAVING A THERAPEUTIC OR PREVENTIVE EFFECT AGAINST A DISEASE RELATED TO EL ACTIVITY AND A METHOD FOR SCREENING AN INHIBITOR OF EL ACTIVITY - The present invention is related to a method for evaluation of drug efficacy of a medicine having a therapeutic or preventive effect against a disease related to EL activity wherein phosphatidylinositol or lysophosphatidylinositol is used as an indicator. The present invention is also related to a method for screening an inhibitor of EL activity using phosphatidylinositol and a kit for use in the method. | 10-15-2015 |
20150353987 | Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same - A device and method for the rapid on-site detection of inhibitors of enzymes, such as, acetylcholinesterase is described where the device contains 2 reaction zones containing a reporter enzyme substrate. One reaction zone is for the test sample while the other is for an onboard negative control. Sample and control fluids are preincubated with the enzyme in separate reaction containers, then an aliquot of each reaction mixture is added to designated reaction zones on the test device. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter. | 12-10-2015 |
20160002695 | ELECTROCHEMILUMINESCENCE (ECL) DETECTION REAGENTS AND RELATED METHODS FOR MEASURING ENZYME ACTIVITY - Disclosed are methods of measuring enzyme activity in a sample. The methods use disulfide-containing detection reagents with an electrochemiluminescent functional group. The present disclosure relates generally to the development of detection reagents and electrochemiluminescence (ECL)-based assays, and reagent test kits for the detection and the quantitative measurement of enzymes in a biological sample. In particular, the present disclosure relates to methods of detecting and measuring enzyme activity in a sample using disulfide-containing detection reagents with an electrochemiluminescent functional group. | 01-07-2016 |
20160018409 | IMPROVED METHOD OF MAPPING GLYCANS OF GLYCOPROTEINS - The use of anthranilic acid (2-AA) to label N-glycans prior to separation using a reversed-phase liquid chromatography (RP-LC) column under acidic conditions using formic acid is described herein. Negatively charged 2-AA offers stronger retention on the reversed phase column than 2-aminobenzamide (2-AB) in RP-LC and allows efficient ionization and detection of 2-AA labeled N-glycans. The acidic conditions used for the RP-LC leads to an efficient separation of acidic 2-AA N-glycans carrying terminal sialylation without the need for an ion-pairing reagent. The method and compositions described herein may be used with RP-nano-LC-MS and a 96-well plate sample preparation, which allows attomolar sensitivity and high throughput. | 01-21-2016 |
20160024553 | MEASUREMENT METHOD FOR HUMAN PANCREATIC LIPASE ACTIVITY - A measurement method for human pancreatic lipase activity in a sample, includes a contact step of bringing a bile acid that makes a pH for giving a maximum value of human pancreatic lipase activity to be lower than 7.7, a diglyceride and a colipase into contact with the sample at pH 7.4 or lower; and a detection step of detecting a signal amount varying in accordance with the human pancreatic lipase activity in the sample, and the bile acid is a bile acid containing: one of or two or more of a-type bile acids selected from the group consisting of GDCA, GCDCA, TDCA, TCDCA and salts thereof; and/or a combination of one of or two or more of b-1-type bile acids selected from the group consisting of GCA, GUDCA, TCA, TUDCA and salts thereof, and one of or two or more of b-2-type bile acids selected from the group consisting of DCA, CDCA and salts thereof. | 01-28-2016 |
20160032353 | IN VITRO ASSAY BUFFER FOR CAS9 - Provided herein is a reaction mixture comprising Cas9 and a non-ionic surfactant, e.g., a polyoxyethylene surfactant. In certain embodiments, the reaction mixture may comprise a Cas9 protein, a guide RNA, a salt, a buffering agent, a nucleic acid target and a non-ionic surfactant. Kits are also provided. In certain embodiments, a kit may comprise: a Cas9 protein; and a concentrated reaction buffer comprising salt, a buffering agent and a non-ionic surfactant. | 02-04-2016 |
20160032354 | METHOD FOR MEASURING INDOXYL SULFURIC ACID - It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods. | 02-04-2016 |
20160083702 | NOVEL PHOSPHOTRIESTERASE ENZYMES, METHODS AND COMPOSITIONS RELATED THERETO - The instant invention provides methods and related compositions for identifying polypeptides with improved stability and/or enzymatic activity in comparison to native forms, wherein the identified polypeptides comprise one or more non-natural amino acids. In certain embodiments, the present invention relates to novel phosphotriesterase enzymes comprising one or more non-natural amino acids. In a particular embodiment, the instant invention provides novel phosphotriesterase enzymes with greater stability and/or enhanced activity in comparison to native forms of the enzyme. The present invention also relates to compositions comprising novel phophotriesterase enzymes, such as prophylactics, decontaminants, animal feedstocks, and assay kits. | 03-24-2016 |
20160083769 | MASS-SPECTROMETRIC RESISTANCE DETERMINATION BY GROWTH MEASUREMENT - The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic. | 03-24-2016 |
20160083770 | ARTICLES AND METHOD FOR DETECTING LISTERIA MONOCYTOGENES - A method of detecting | 03-24-2016 |
20160151756 | COMPOSITIONS AND METHODS FOR ARRANGING COLLOID PHASES | 06-02-2016 |
20160177370 | METHOD FOR DETECTING STREPTOCOCCUS AGALACTIAE USING ESTERASE ACTIVITY | 06-23-2016 |
20160194682 | LEUKOCYTE ESTERASE DETECTION FROM THROAT SWAB | 07-07-2016 |