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Involving hydrolase

Subclass of:

435 - Chemistry: molecular biology and microbiology

435004000 - MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP

Patent class list (only not empty are listed)

Deeper subclasses:

Class / Patent application numberDescriptionNumber of patent applications / Date published
435023000 Involving proteinase 244
435019000 Involving esterase 101
435024000 Involving peptidase 46
435022000 Involving amylase 14
Entries
DocumentTitleDate
20100021950SCREENING METHOD FOR ANTI-DIABETIC COMPOUNDS - The present invention relates to a method of identifying a compound suitable as a lead compound and/or as a medicament for the treatment and/or prevention of diabetes, the method comprising the steps of (a) contacting a test compound with a cell comprising a protein, wherein said protein (i) comprises or consists of the amino acid sequence of any one of SEQ ID NOs: 1 to 29; (ii) is encoded by a nucleic acid molecule comprising or consisting of the sequence of any one of SEQ ID NOs: 59 to 87; (iii) is a fragment of the protein according to (i) or (ii) and exhibits Eph-Receptor-Tyrosine-Kinase activity; or (iv) has a sequence at least 75% identical with the protein according to (i) or (ii) or with the fragment according to (iii) and exhibits Eph-Receptor-Tyrosine-Kinase activity; and (b) determining whether said test compound, upon contacting in step (a) inhibits the Eph receptor tyrosine kinase activity of said protein wherein said inhibition indicates a compound suitable as a lead compound and/or as a medicament for the treatment and/or prevention of diabetes.01-28-2010
20090170145MODIFIED CREATININE AMIDE HYDROLASE HAVING IMPROVED AFFINITY FOR SUBSTRATE, AND REAGENT COMPOSITION FOR DETERMINATION OF CREATININE - [Problems] To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency.07-02-2009
20120183982NON-INVASIVE DIAGNOSTIC METHOD FOR THE EVALUATION OF INTESTINAL LACTASE DEFICIENCY (HYPOLACTASIA) - The test of the invention comprises the measuring the total amount of xylose in urine and/or its concentration in blood following oral administration of 4-O-β-D-galactopyranosyl-D-xylose (4-GX) to the patient. It is a non-invasive test that is based on the direct evaluation of the global enzyme activity in the whole individual, not on measuring the metabolic consequences derived from its deficiency. It does not require specialised equipment, does not cause apparent discomfort in patients with lactase deficiency and is very reliable, thus overcoming the drawbacks of the diagnostic tests currently in use and is a statistically significantly better test in terms of its reliability; consequently it should become the reference or gold standard test for the indication of hypolactasia.07-19-2012
20100075357FRET PROTEASE ASSAYS FOR CLOSTRIDIAL TOXINS - The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.03-25-2010
20100075356ANALYSIS OF PROTEOLYTIC PROCESSING BY MASS SPECTROMETRY - The present invention relates to the simultaneous analysis of samples for determining differential proteolytic processing based on isotopic labelling of N-terminal peptides, wherein the isotopic labelling is achieved by incorporation of 03-25-2010
20100075355ULTRA-SENSITIVE DETECTION OF ENZYMES BY CAPTURE-AND-RELEASE FOLLOWED BY QUANTIFICATION - The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.03-25-2010
20100105094METHOD, DEVICE AND APPARATUS FOR MEASURING THE CONCENTRATION OF CREATININE, AND METHOD, DEVICE AND APPARATUS FOR MEASURING THE AMOUNT OF SALT IN URINE USING THE SAME - A method for measuring the concentration of creatinine includes the steps of: (A) mixing a sample containing creatinine with a creatinine quantitative reagent including 1-methoxy-5-methylphenazinium in the absence of picric acid and any enzyme responsive to creatinine, to cause the creatinine to reduce the 1-methoxy-5-methylphenazinium; (B) electrochemically or optically measuring the amount of the 1-methoxy-5-methylphenazinium reduced in the step (A); and (C) determining the concentration of the creatinine contained in the sample from the amount of the reduced 1-methoxy-5-methylphenazinium measured in the step (B).04-29-2010
20130029365MULTI-DIMENSIONAL CHROMATOGRAPHIC METHODS FOR SEPARATING N-GLYCANS - A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.01-31-2013
20120164672HIGH-THROUGHPUT IN VITRO TRANSLATION (CELL-LYSATE BASED) ASSAY FOR DETECTING QUORUM SENSING SIGNALS - A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., 06-28-2012
20090275066IMMOBILIZATION OF MEMBRANE PORTEINS ONTO SUPPORTS VIA AN AMPHIPHILE - The invention pertains to the field of membrane protein immobilization onto supports. It relates to a product comprising a support and at least one membrane protein attached to the surface thereof, characterized in that said membrane protein is attached to said support using an amphiphilic molecule with which said membrane protein is complexed. It also relates to a process for preparing such product, as well as to various applications in the fields of diagnosis, drug design and biotechnologies. It further relates to a kit, together with a functionalized amphiphilic molecule, for preparing a product according to the invention comprising a support and an amphiphilic molecule, wherein the amphiphilic molecule and the support interact through a hydrophobic bond, an ionic bond, a specific bond or a covalent bond.11-05-2009
20090269792ACETAMINOPHEN ASSAY - In general, the present invention provides a reliable assay for the quantitative determination of p-aminophenol in an aqueous sample. More particularly, the present invention provides a rapid enzyme-based assay for the quantitative determination of acetaminophen in a sample. The assay employs a xylenol chromophore and a catalyst that is preferably a weak oxidizer. The assay provides reliable results in the presence or absence of N-acetylcystiene (NAC) and can therefore be used to monitor acetaminophen levels during NAC treatment. Methods and kits for determining acetaminophen concentration in an aqueous sample are also provided.10-29-2009
20130065263FLUORESCENT SUBSTRATE FOR DETECTION OF ENZYMATIC ACTIVITY OF NITRILE-RELATED ENZYME - The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme.03-14-2013
20090047698Reaction medium for Vibrio bacteria - The present invention relates to a reaction medium for 02-19-2009
20100041086Enzyme Assays for a Droplet Actuator - The invention relates to a microfluidic platform and methods of using the platform for conducting enzyme assays using a droplet actuator. The enzyme assays of the invention are useful for, among other things, identifying and/or characterizing disorders resulting from conditions in which enzymes are defective or are produced in inappropriate amounts. Enzyme assays of the invention may, for example, be used to detect altered activity of a particular enzyme in a sample, which may serve as an indicator of a particular disease. Altered activity may, for example, be caused by conditions which result in the increased or reduced production of a certain enzyme or its substrate and/or conditions which result in defective enzymes and/or substrates exhibiting increased or decreased effectiveness relative to corresponding normal enzymes and/or substrates.02-18-2010
20110045516Kits Pertaining to Analyte Determination - This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.02-24-2011
20100267068ANALYSIS OF DOPING COMPOUNDS - Process for determination of a drug or medicament or their metabolites in a (body) fluid or an extract of (body) tissue or (body) sample comprising the steps: (i) optionally treatment of the fluid or sample extract with at least one enzyme, (ii) contacting at least a first part of the fluid or extract with at least one first solid phase able to adsorb or absorb organic compounds out of the fluid or extract, and/or (iii) contacting at least a second part of the fluid or extract with at least one second phase, able to extract or adsorb organic compounds out of the fluid or extract, and/or (iv) optionally contacting at least a third part of the fluid or extract with a liquid phase able to extract organic compounds out of the fluid or extract, and/or (v) desorbing the compound(s) bound to the first solid phase by applying heat to the solid phase and/or by solvent extraction, optionally followed by evaporating the solvent at least partially, (vi) optionally extracting or desorbing the compound(s) contained in or bound to the second phase by applying heat and/or by solvent extraction, optionally followed by evaporation of the solvent at least partially and (vii) analyzing the desorbed compounds of steps (v) and (vi) and optionally the compounds extracted in step (iv) by multi-dimensional gas chromatography (GC), preferably by comprehensive multi-dimensional GC (GC*GC) coupled preferably to a mass spectrometry.10-21-2010
20090221014Enzymatic Biosensors With Enhanced Activity Retention For Detection Of Organic Compounds - Enzymatic biosensors and methods of producing distal tips for biosensor transducers for use in detecting one or more analytes selected from organic compounds susceptible to dehalogenation, organic compounds susceptible to oxygenation, organic compounds susceptible to deamination, organosulfate compounds susceptible to hydrolysis, and organophosphate compounds susceptible to hydrolysis are disclosed herein, as well as biosensor arrays, methods of detecting and quantifying analytes within a mixture, and devices and methods for delivering reagents to enzymes disposed within the distal tip of a biosensor.09-03-2009
20110300566METHODS FOR SCREENING FOR HISTONE DEACETYLASE ACTIVITY AND FOR IDENTIFYING HISTONE DEACETYLASE INHIBITORS - The present invention relates to novel methods for screening for histone deacetylase enzyme activity in a test sample. The present invention further relates to novel methods for screening potential inhibitors of histone deacetylase enzymes.12-08-2011
20090221013METHOD FOR QUANTIFYING LIVING COLIFORM MICROORGANISMS IN A WATER SAMPLE - The invention relates to a method of rapidly quantifying living coliform microorganisms in a sample of water in which the microorganisms are put into contact with a fluorogenic substrate that is hydrolyzed in methylumbelliferone by an enzyme given off by the microorganisms. Methylumbelliferone is detected by the fluorescence that it emits, with the rate at which this fluorescence varies being measured in order to determine the concentration of coliform microorganisms in the original sample on the basis of the calculated rate of variation and a correlation plot. In characteristic manner, a preliminary treatment step is performed on the sample, in particular pre-filtering, serving to remove at least some of the particles in suspension in the water, thus making it possible to achieve quantification that is entirely comparable with the quantification given by the standardized multi-well panel method. The invention is applicable to analyzing bathing water for detecting 09-03-2009
20110287465CHARACTERIZATION OF O-LINKED GLYCANS - The present disclosure provides methods for analyzing structure and/or composition of glycoproteins and glycans of glycoproteins. Such methods can include subjecting a glycoprotein preparation to a condition that removes at least one O-linked glycan from the glycoprotein. Such methods can include subjecting a glycoprotein preparation to a condition that releases an N-glycan from the glycoprotein, e.g., prior to subjecting the glycoprotein to a condition that releases an O-glycan from the glycoprotein.11-24-2011
20090203057PROTEIN DEMETHYLASES COMPRISING A JMJC DOMAIN - Post-translational modification, including protein methylation, plays an important role in regulating protein function. The present invention provides a novel assay for evaluating demethylase activity and the discovery of a family of protein demethylases comprising a novel demethylase motif.08-13-2009
20110287464Chromogenic plating media for the identification of Enterobacter sakazakii - A plating medium for identification of Enterobacter sakazakii bacteria having a carbohydrate, but Enterobacter sakazakii bacteria being incapable of fermenting any carbohydrate in the medium. The medium also contains a pH indicator dye that changes the color of the medium from a first color to a second color when the pH changes, first and second chromogenic substrates that react to alpha-glucosidase and beta cellobiosidase enzymes, respectively, to produce a third color in the medium, and agar to solidify the mixture. Microorganisms that ferment the carbohydrate but do not produce alpha-glucosidase or beta-cellobiosidase will produce colonies of the second color, microorganisms that produce alpha-glucosidase and/or beta-cellobiosidase including Enterobacter sakazakii bacteria will produce colonies of the third color, and microorganisms that ferment the carbohydrate and produce alpha glucosidase and/or beta-cellobiosidase will produce colonies of a fourth color which is the color that results from mixing the second and third colors.11-24-2011
20110287463RECOMBINANT BETA-GALACTOSIDASE DERIVED FROM STREPTOCOCCUS PNEUMONIAE - The present invention relates to a novel beta-galactosidase derived from 11-24-2011
20100035290ENZYME DETECTION SYSTEM WITH CAGED SUBSTRATES - An enzyme detection method includes forming a caged substrate; releasing an uncaged substrate by cleaving a caging molecules from the caged substrate; and emitting a light emission from a Bioluminescence Resonance Energy Transfer luminescent nanocrystal conjugate reacting with the uncaged substrate.02-11-2010
20090098588BETA GALACTOSIDASE DONOR FRAGMENTS - Truncated fragments of the small fragment of β-galactosidase are provided that have low affinity for the large fragment of β-galactosidase and provide for robust signals when two fusion proteins are complexed due to the binding of the proteins to which the β-galactosidase fragments are fused. The truncated fragments do not interfere with the complexing of the two proteins and allow for the two proteins to function and be responsive to candidate compounds that affect complex formation.04-16-2009
20100267069ANALYSIS OF PROTEINS FROM BIOLOGICAL FLUIDS USING MASS SPECTROMETRIC IMMUNOASSAY - Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states.10-21-2010
20120107853Use of Genetically Modified Organisms to Generate Biomass Degrading Enzymes - The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.05-03-2012
20090263843Protein labeling with cyanobenzothiazole conjugates - The invention provides compounds and methods for site-specifically labeling proteins with cyanobenzothiazole derivatives of formula I. For example, the invention provides methods for labeling the N-terminus of a protein that terminates with a cysteine residue. The invention also provides methods for isolating an N-terminally labeled protein and methods for detecting an N-terminally labeled protein.10-22-2009
20090263842METHOD OF ASSESSING THE METASTATIC STATUS OF A PRIMARY TUMOR - The current invention provides a method for determining the metastatic potential, capability, status, or characteristics of a primary tumor from a human cancer patient by determining expression patterns of proteins that initiate, cause, promote, mediate, inflict, or otherwise aid metastatic properties of cells from a primary tumor. The identification of one or more proteins associated with metastasis in a single primary tumor can determine whether a primary tumor is metastatic or has the potential to become metastatic.10-22-2009
20110171674Beta-Glucosidase Variants Having Improved Activity, and Uses Thereof - The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of 07-14-2011
20090275065METHODS AND COMPOUNDS FOR DETECTING BETA-LACTAMASE ACTIVITY - The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z-R11-05-2009
20100279328Fret assays, methods for performing the assays and compounds relevant to the assays - The present invention is generally directed to biological assays. More specifically it is directed to FRET-based assays using particularly effecting FRET pairs, methods for performing such assays and the molecules utilized in the assays. In a composition aspect, the present invention is directed to a FRET-based protease substrate selected from the list of substrates shown in the Detailed Description section above. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET-substrate is selected from the list of substrates shown in the Detailed Description section above. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description section above.11-04-2010
20110171673COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.07-14-2011
20100279330METHOD FOR DETECTING AND/OR IDENTIFYING CLOSTRIDIUM DIFFICILE - The invention relates to a method for detecting and/or identifying 11-04-2010
20130217056QUANTIFICATION OF NON-REDUCING END GLYCAN RESIDUAL COMPOUNDS - Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.08-22-2013
20120295292Detecting Protein Arginine Deiminase (PAD) Activity in Human Tissues and Sera - Methods for diagnosing disease that is characterized by an overabundance of citrullinated proteins such as colitis, rheumatoid arthritis, and/or malignant cancer in a subject are provided. The PAD protein activity level can be measured in a tissue sample (e.g., serum) of the subject and then compared to a control PAD protein activity range of a control group. A finding of an increased PAD protein activity level in the tissue sample of the subject compared to the PAD protein activity level of the control group is indicative of disease characterized by an overabundance of citrullinated proteins in the subject.11-22-2012
20100105093ASSAY METHOD FOR THE DETECTION OF VIABLE MICROBIAL CELLS IN A SAMPLE - The present invention discloses an assay method for the detection of viable microbial cells in a sample, the assay method comprising the steps of: i) adding an ATP degrading enzyme to a sample suspected of containing viable microbial cells to substantially degrade any extracellular ATP in the sample; ii) adding a phosphate containing compound to the sample to substantially halt action of the ATP degrading enzyme; and iii) subjecting the sample to a detection assay to establish the level of undegraded ATP in the sample to provide an indication of the level of viable microbial cells in the sample.04-29-2010
20090170143Determination of Neutrophil Gelatinase-Associated Lipocalin (NGAL) as a Diagnostic Marker for Renal Disorders - Methods for diagnosing renal disorders by measuring human neutrophil gelatinase-associated lipocalin (NGAL) are provided.07-02-2009
20080274488Covalent tethering of functional groups to proteins and substrates therefor - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.11-06-2008
20090170144DETERMINATION OF VIABLE MICROORGANISMS USING COATED PARAMAGNETIC BEADS - The present invention relates to methods for the detection of microorganisms. In one embodiment, the present invention provides methods for detecting live microorganisms in a culture by capturing and culting the microorganisms on para-tropic-coated paramagnetic beads. This technique is useful for any application in which it is necessary to monitor the biological contamination level, for example drinking water, recreational waters, food processing waters and medical laboratories. In one embodiment, the method for determining the concentration of viable microorganisms in a sample according to the invention further comprises an inducer reagent, wherein the inducer reagent includes an inducer compound that induces the activity of an enzyme unique to the microorganism of interest.07-02-2009
20110008810DIAGNOSTIC METHOD OF MUCOPOLYSACCHARIDOSES - Provision of a method for accurate diagnosis of mucopolysaccharidoses, including determining the level of glycosaminoglycan in a biological sample with high sensitivity and with ease.01-13-2011
20090023172GTP Cyclohydrolase II as a Target for Fungicides - The present invention relates to the identification of fungal GTP cyclohydrolase II as a target for fungicides, to a method for identifying antifungal agents based on fungal GTP cyclohydrolase II, and also to the use of compounds identified as fungicides via the abovementioned method.01-22-2009
20090023170Methods And Kits For The Detection of Biotoxic and Antibiotic Residues - The invention provides a kit for the detection of antibiotic residues comprising inducible bacterial spores which produce an induced enzyme after germination and induction; a germinant that triggers rapid germination of said spores; an inducer that triggers the production of the specific enzyme of interest; a substrate upon which said enzyme acts; and a detector of the activity of said enzyme on said substrate.01-22-2009
20080233604Methods For Identifying Compounds Capable of Modulating the Hydrolase Activity of Clca Protein - Methods for identifying compounds capable of modulating the hydrolase activity of a CLCA protein include screening and computer modelling methods. The compounds, including antibodies, may be useful as therapeutic agents to treat a variety of diseases.09-25-2008
20090053745Methods and Kits for Regulation of Microtubule Assembly and Dendrite Growth and Branching - Fragments of snapin important for interaction with cypin and thus regulating microtubule assembly are provided. Also provided are methods of use of said fragments and kits to facilitate said methods.02-26-2009
20090117600Detection of biological molecules by differential partitioning of enzyme substrates and products - This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.05-07-2009
20110229918Method of Quantifying Transient Interactions Between Proteins - The invention relates to a precursor for producing sintered metallic components, a method for producing the precursor and the production of the components. The object of the invention is to disclose possibilities of being able to produce sintered metallic components, which render possible an increased physical density and a reduced shrinkage on the fully sintered component. With a precursor according to the invention for the production of sintered metallic components, a coating layer is formed on a core, which is formed from respectively one particle of a first metallic powder. The coating layer is formed with a second powder and a binder. The first powder thereby has a particle size d09-22-2011
20090117601METHODS AND COMPOSITIONS FOR THE DETECTION OF BETA-LACTAMASES - Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases. AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.05-07-2009
20100159494Methods for determining cellulolytic enhancing activity of a polypeptide - The present invention relates to methods for determining cellulolytic enhancing activity of a polypeptide, comprising: (a) incubating a cellulosic material with an enzyme composition comprising a cellobiose dehydrogenase and one or more (several) cellulolytic enzymes in the presence and absence of the polypeptide; and (b) measuring the release of sugar from the cellulosic material in the presence and absence of the polypeptide.06-24-2010
20100190194POSTPRANDIAL HYPERGLYCEMIA MARKER, METHOD OF MEASURING THE SAME, AND USAGE THEREOF - A new method of diagnosing postprandial hyperglycemia by indirectly measuring a blood glucose level is provided. Postprandial hyperglycemia is detected by measuring a glycation degree of lysine in hemoglobin, in which a side chain amino group of lysine is glycated (GHbLys %). Measurement of GHbLys % can be performed by cleaving hemoglobin by protease, treating a glycated part of a lysine residue in the obtained cleavage product of hemoglobin with fructosyl amino acid oxidase, and measuring a redox reaction between the glycated part and fructosyl amino acid oxidase.07-29-2010
20100151507Cavity Induced Allosteric Modification Of Intermolecular Interactions And Methods Of Identifying Compounds That Effect The Same - Method of identifying compounds that modulate intermolecular interactions between a target protein and a modifier are disclosed. Pharmaceutical composition comprising compounds that inhibit intermolecular interactions between a target protein and a modifier are disclosed. Methods of treating individual suffering from inflammatory conditions, undesirable immune responses, immunological conditions and bacterial infections are disclosed.06-17-2010
20100143957METHODS OF MEASURING ADAMTS13-MEDIATED IN VIVO CLEAVAGE OF VON WILLEBRAND FACTOR AND USES THEREOF - The invention generally relates to methods of measuring cleaved von Willebrand factor (VWF) fragments. More specifically, the invention relates to methods of measuring the ability of a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) to cleave VWF in vivo. The invention also relates to methods of using various animal models which demonstrate ADAMTS13 activity similar to that of a human. The invention further relates to methods of measuring the cleavage products of rVWF in mammals, particularly in humans and in human plasma.06-10-2010
20100255519Salivary Bioassay for Early Detection of Bone Loss - The present invention is directed to methods to detect and diagnose osteoporosis and periodontal disease using salivary biomarkers.10-07-2010
20120196309Methods and Kits for Detection of Antibiotic Resistance - The present invention relates to a method of detecting antibiotic resistant bacteria in a sample. The method includes the steps of analyzing a sample derived from bacteria via mass spectrometry to produce a data set, and determining from the data set the presence or absence of a covalently modified antibiotic compound in the sample, wherein the presence of a covalently modified antibiotic compound in the sample is indicative that the bacteria are resistant to the antibiotic. The present invention also relates to a kit for determining the presence or absence of antibiotic resistant bacteria in a sample. The kit includes reagents for preparing and performing the assay, and instructions for the set-up, performance, monitoring, and interpretation of the assay.08-02-2012
20100112619LIGAND-CONTAINING MICELLES AND USES THEREOF - Ligand-containing micelles and various compositions, kits and methods for their preparation and use are provided.05-06-2010
20100112620BIORHYTHM INFORMATION ACQUISITION METHOD - A method of acquiring information on a biorhythm on the basis of a variation with time of the quantity of a physiologically active substance in tear and/or saliva is provided.05-06-2010
20110151492METHOD OF DETECTING ARTICULAR CARTILAGE DEGENERATION OR DAMAGE - An object of the present invention is to provide a detection method capable of detecting articular cartilage degeneration or damage in which the abnormality cannot be detected in a radiograph in a simple method and with high accuracy, a method of evaluating the rate of progression of articular cartilage degeneration or damage, and the like.06-23-2011
20110111442Intein-modified enzymes, their production and industrial applications - A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.05-12-2011
20090068695Sir2 products and activities - A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD03-12-2009
20110244499METHODS OF ENZYMATIC HYDROLYSIS - In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass with acid and heat under conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Next, at least a portion of the cellulose of step (a) is enzymatically hydrolyzed under conditions sufficient to form a composition comprising glucose. The glucose is then fermented. Advantageously, one or more reaction conditions are more efficient because they are selected by first measuring an initial hydrolysis rate of said biomass and then selecting one or more appropriate reaction conditions based upon said initial hydrolysis rate.10-06-2011
20100081154IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES - A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.04-01-2010
20110250626Visual Assays for Coatings Incorporating Bioactive Enzymes for Catalytic Functions - Disclosed herein are materials such as a coating, comprising a lipolytic enzyme or organophosphrous compound degrading enzyme. Also disclosed herein are methods of visually detecting enzyme activity in a coating by contacting the coating with a substrate of an enzyme and a visual indicator that changes appearance upon production of a product of enzyme activity on a tack-free coating surface.10-13-2011
20100055726ASPARAGINASES - The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.03-04-2010
20100062468METHODS AND PRODUCTS RELATED TO LOW MOLECULAR WEIGHT HEPARIN - The invention relates to methods and products for characterizing and using polysaccharides. Low molecular weight heparin products and methods of use are described. Methods for characterizing purity and activity of polysaccharide preparations including glycosaminoglycans such as heparin are also described.03-11-2010
20100062465Detection of Histone Deacetylase Inhibition - Provided are compositions and methods for intracellular detection of enzyme activity. One example of a composition is a histone deacetylase substrate comprising a compound of the following formula (I): One example of a method is a method for detecting histone deacetylase activity comprising introducing a compound according to formula (1) to a plurality of cells and monitoring the cells with magnetic resonance spectroscopy.03-11-2010
20100062466MEDIUM FOR THE DETECTION AND/OR IDENTIFICATION OF BACTERIA - A detection medium including 03-11-2010
20100068744Method for the purification of heat shock proteins - Recombinant purified DnaK—having a ATPase activity without the addition of an other chaperone protein—essentially free of T-cell stimulating impurities.03-18-2010
20100068745PROCESS AND KIT FOR MEASURING THE CONDITION OF THE FIBRINOLITIC SYSTEM - The invention relates to an assay and a kit for the determination of the status of the fibrinolytic system. In particular the invention relates to a method for the determination of the status of the fibrinolytic system from tear fluid samples. Additionally the invention relates to a sterilized, calibrated capillary equipped with a plastic balloon, suitable for tear fluid sampling, and a kit for the assessment of the status of the fibrinolytic system, or for the determination of its balance, comprising a sampling capillary, reaction sheets, color scale to help the evaluation, and reagents03-18-2010
20090215100Process for producing sugar chain derivative, structure analysis method, and sugar chain derivative - A process for preparing an oligosaccharide derivative from an oligosaccharide mixture, the process being characterized in that the process comprises the steps of (a) introducing a lipophilic group into oligosaccharides of the mixture to obtain a mixture of oligosaccharide derivatives, and (b) treating the oligosaccharide derivative mixture by serotonin affinity column chromatography.08-27-2009
20110033881SYSTEM FOR DETECTING MICROBIAL CONTAMINATION - The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hyperbaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to said hyperbaric chamber, and (iii) a means for pressurizing said hyperbaric chamber, said system further comprising a kit for detection of micro-organisms adsorbed to said adsorbent material, wherein said kit is based on enzymatic detection using chromogenic and/or fluorescent substrate analogues.02-10-2011
20100311094Allosteric Modulation Of Ship Polypeptides And Uses Thereof - This invention provides a method for identifying allosteric modulators of a SHIP polypeptide, wherein said SHIP polypeptide is contacted with a test compound, and wherein said SHIP polypeptide comprises an allosteric site selected from the group consisting of a SHIP C2 domain and a SHIP PH domain.12-09-2010
20100297682METHOD OF DIAGNOSTIC RHEUMATOID ARTHRITIS BY SUGAR CHAIN ANALYSIS - Provided is a novel method of diagnosing rheumatoid arthritis. Based on the quantitative expression profile of sugar chain expression amount in the serum (whole serum, HAP or LAP), the relevancy thereof to rheumatoid arthritis is analyzed. As a result, a sugar chain and a glycoprotein showing a change depending on the onset of rheumatoid arthritis are found out and thus a serum sugar chain and a glycoprotein usable as a novel biomarker are provided.11-25-2010
20080268487Method For Identifying Agents Which Modulate GtpPase Activity Involved in Insulin-Stimulated Glut4 Translocation - The present invention is a method for identifying agents that modulate the GTPase activity of AS160. In the instant assay, AS160 or the GAP domain thereof is contacted with a test agent, in the presence of GTP-bound Rab (2A, 8A, 8B, 10, or 14), and the AS160 GAP domain-mediated hydrolysis of GTP to GDP is monitored.10-30-2008
20110165604REACTION MEDIUM FOR METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) BACTERIA - A reaction medium for detecting and/or identifying Methicillin-resistant 07-07-2011
20120064557METHOD OF IDENTIFYING METALLO-BETA-LACTAMASE-PRODUCING BACTERIA - An object of the present invention is to provide a method of identifying metallo-β-lactamase-producing bacteria, particularly, a method of conveniently identifying an IMP or VIM type. According to the present invention, there is provided a method of detecting metallo-β-lactamase-producing bacteria, comprising spotting a compound (I) onto a surface of a solid medium coated with the bacteria to be tested, spotting 3 types of β-lactam agents at 3 respective positions different from the spot of the compound (I), culturing the solid medium, and then detecting the metallo-β-lactamase-producing bacteria based on the shape of an inhibition zone formed around the spot of each of the β-lactam agents. There is further provided a method of identifying an IMP or VIM type of the metallo-β-lactamase-producing bacteria.03-15-2012
20110008809METHOD AND SYSTEM FOR ENZYMATIC DETECTION OF MELAMINE - Melamine is a common industrial chemical contaminant which should be absent from food and feed supplies due to melamine's toxicity. Provided is a method to assess the presence of melamine in samples prepared from compositions. The method may include using a microbial enzyme called melamine deaminase which hydrolyzes melamine to ammeline and ammonia. The method may include assessing the presence of any ammonia produced from an enzymatic reaction between the sample and the enzyme.01-13-2011
20110053195CATALYTICALLY INACTIVE PROTEINS AND METHOD FOR RECOVERY OF ENZYMES FROM PLANT-DERIVED MATERIALS - An inactive xylanase molecule for the recovery of xylanase activity in plant-derived material containing active xylanase enzyme(s) and xylanase inhibitors. The inactive xylanase molecule of binds to xylanase inhibitors in the plant-derived material, thereby allowing accurate measurement of xylanase enzyme activity of the enzyme contained in the plant-derived material. The invention further includes amino acid molecules depicted by SEQ ID NOS. 4 through 112, wherein the catalytically active sites of each of the amino acids have been modified resulting in inactive xylanase molecules. A method of production of the inactive xylanase molecules includes expression of the inactive xylanase molecule in microbial or eukaryal (e.g., yeast including 03-03-2011
20110027815METHOD OF DETECTING ISOLEVUGLANDIN PHOSPHOLIPID ADDUCTS - A method of measuring isolevuglandin and/or levuglandin adducts associated with oxidative injury in a subject includes obtaining a bodily sample from a subject suspected of including isolevuglandin and/or levuglandin phospholipid adducts, hydrolyzing isolevuglandin and/or levuglandin phospholipid adducts from the sample with an enzyme that forms isolevuglandin and/or levuglandin phospholipid derivatives, and determining the amount of isolevuglandin and/or levuglandin phospholipid derivatives by mass spectrometry.02-03-2011
20100285513Methods of Analyzing Peptide Mixtures - The present invention provides for a method of characterizing and classifying a sample of peptide or polypeptide mixtures or a biomolecule comprising a polypeptide component by using mass spectrometry and statistic methods for analyzing the mass spectrometry results.11-11-2010
20100159493METHODS OF QUANTIFYING METHOTREXATE METABOLITES - The present invention provides a method for efficiently converting methotrexate polyglutamates to methotrexate in a cellular extract by contacting the cellular extract with gamma glutamyl hydrolase under conditions suitable for efficient conversion of methotrexate polyglutamates to methotrexate.06-24-2010
20100196939Diagnosis of Conditions Associated with Decreased Arginine Bioavailability - The invention features methods and compositions for diagnosis, including prognosis, of conditions associated with decreased arginine bioavailability (which can result from dysregulated arginine metabolism, e.g., due to increased arginase activity) by assessing in a sample from a subject the ratio of arginine to one or more, usually two or more, modulators of arginine bioavailability. In one embodiment, the ratio of arginine to (ornithine+citrulline) is assessed to aid in diagnosis.08-05-2010
20090203055Compositions and methods for RNA interference with sialidase expression and uses thereof - The present invention provides RNAi agents targeted to sialidase. The RNAi agents include siRNA, shRNA, and expression vectors that comprise a template for transcription of an siRNA or shRNA. The invention further provides cells and cell lines that comprise an RNAi agent targeted to sialidase. The cells and cell lines exhibit reduced sialidase activity relative to control cells that do not comprise an RNAi agent targeted to sialidase. Certain of the cell lines stably express the RNAi agent. The invention further provides methods of producing the cells and cell lines. The invention further provides methods for producing a glycoprotein in cells that comprise an RNAi agent targeted to sialidase. The glycoproteins exhibit an improved sialic acid profile relative to glycoproteins produced by cells that do not comprise an RNAi agent targeted to sialidase. The invention further provides glycoproteins, e.g., therapeutic glycoproteins, produced in the cells.08-13-2009
20090203056KIT FOR DIAGNOSIS OF CANCER - The present invention relates to a kit for assaying the activity of the human arylsulphatase or its isozymes, including 4-MUS and 4-MU. The kit can be used for initial screening, therapeutic effect monitoring and recurrence inspecting of human cancers.08-13-2009
20110189711PAAD DOMAIN-CONTAINING POLYPEPTIDES, ENCODING NUCLEIC ACIDS, AND METHODS OF USE - The invention provides isolated nucleic acid molecules encoding PAAD-domain containing polypeptides and functional fragments thereof, including fragments containing PAAD domains, NACHT domains and ARED domains, encoded polypeptides, and antibodies. Also provided are methods of identifying polypeptides and agents that associate with a PAAD-domain containing polypeptide or fragment thereof, or that alter an association of a PAAD domain-containing polypeptides. Further provided are methods of identifying agents that modulate PAAD domain-mediated inhibition of NFκB activity, or modulate an activity of a NACHT domain of a PAAD domain-containing polypeptide. Also provided are methods of modulating NFκB transcriptional activity in a cell, and methods of altering expression of a PAAD domain-containing polypeptide in a cell.08-04-2011
20110189710TREATMENT OF POMPE DISEASE WITH SPECIFIC PHARMACOLOGICAL CHAPERONES AND MONITORING TREATMENT USING SURROGATE MARkERS - Provided is a method of monitoring the treatment of Pompe disease with specific pharmacological chaperones using systemic and/or cellular surrogate markers.08-04-2011
20120040388CELLULASE-CONTAINING DISH DETERGENTS - The present invention provides cellulase-containing dishwashing detergent compositions. The present invention also provides methods for the production of and use of such detergents. In addition, a spaghetti mix soil suitable for testing dishwashing detergents is also provided.02-16-2012
20080248512Methods for detection of lysosomal storage disease - The present invention provides compositions for performing assays of enzyme activity associated with lysosomal storage diseases. The invention further provides methods for determining enzyme activity, and methods for the screening for lysosomal storage disease in an individual.10-09-2008
20100062467Medium For Detecting And/Or Identifying Bacteria - The invention relates to a method for detecting and/or identifying 03-11-2010
20100279331Novel Transporter Protein in Mammal and Utilization of the Same - The present invention provides a lipid membrane that contains a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, or 22. Use of the present invention enables screening for a chemical which regulates excretion of a chemical and/or a waste. Furthermore, use of the present invention enables an arbitrary chemical to be tested for nephrotoxicity and/or hepatotoxicity.11-04-2010
20100279329METHOD FOR THE STABILISATION OF PURIFIED P-GLYCOPROTEIN - There is provided a method for producing purified P-glycoprotein (P-gp) in a stabilised form such that, after being reconstituted into proteoliposomes, it can be freeze dried and stored for prolonged periods of time without loss of biological activity The method comprises the steps of: i) solubilising a mixture of P-gp and other proteins obtained from cell membranes; ii) removing insoluble material by centrifugation; iii) placing the mixture of P-gp and other proteins in a purification column; and iv) running through the purification column at least one wash buffer so as to remove unwanted proteins, and then at least one elution buffer so as to recover the P-gp; wherein one or more of the buffers contains a disaccharide. Trehalose is a particularly preferred disaccharide.11-04-2010
20110306075METHODS RELATED TO MODIFIED GLYCANS - Among other things, the present disclosure provides methods for enriching, identifying, and/or quantifying unusually modified glycans (e.g., phosphorylated glycans, sulfated glycans, and/or multi-acetylated glycans). In many embodiments, methods comprise providing a glycan preparation from which sialic acids have been released; subjecting the sialidase-treated glycan preparation to a separation technique that separates glycans based on charge-to-mass ratio; and quantifying the charged products using at least one quantification standard.12-15-2011
20090023173Luminescence-based methods and probes for measuring cytochrome P450 activity - The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.01-22-2009
20090023171Method for determining specific groups constituting heparins or low molecular weight heparins - The invention provides methods for analysing heparins, low-molecular-weight heparins, ultralow-molecular-weight heparins, and oligosaccharides by high performance liquid chromatography a stationary phase dynamically coated with a quaternary ammonium salt. The methods of the invention may be used to analyse samples without pre-treatment or to analyse samples that have been partially or exhaustively depolymerised and, optionally, reduced. Specific saccharides can be detected.01-22-2009
20100173340USE OF HEMATOPOIETIC GROWTH FACTOR INDUCIBLE NEUROKININ-1 (HGFIN) AS A NOVEL BIOMARKER - The invention provides, in certain embodiments, a method of detecting an indicator of renal injury or renal disease. The method entails assaying a urine sample for hematopoietic growth factor inducible neurokinin-1 (HGFIN), wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of renal injury or renal disease, and/or the rate of loss of renal function. In other embodiments, the invention provides a method of detecting an indicator of systemic inflammation. This method entails assaying a biological sample for HGFIN, wherein the presence of HGFIN at an elevated level indicates the presence and/or degree of systemic inflammation. Also provided, are methods of determining progression of these conditions, as well as methods of determining subjects' response to treatment.07-08-2010
20120045785Hybrid Prokaryotic-Eukaryotic Tubulins and Use Thereof - The present invention embraces hybrid 02-23-2012
20120064556LIPOPROTEIN LIPASE ASSAY - The present invention is directed to a method and kit for the measurement of LPL. The method comprises the following steps; administering a dosage of an anti-coagulant to the subject, gathering the biological sample, adding the biological sample to a well of a well plate, adding a first buffer to the well plate, adding a second buffer to the well plate, adding a substrate to the well plate, incubating the well plate, measuring the fluorescence of the material within the well plate, and correlating the measured fluorescence to a level of LPL activity. The kit comprises the following elements; an anti-coagulant, a well plate, a first buffer, a second buffer, a substrate, and a correlator.03-15-2012
20120045784BIOTINIDASE ASSAY - The present technology discloses biotinidase assay, biotinidase substrates (I) and a kit wherein the biotinidase substrate includes a label molecule separated from the biotin carbamoyl group by a linker X longer than about 4 Å but shorter than about 27 Å.02-23-2012
20090325206Method for assaying the activity of lysosomal enzymes - A method, and associated kit, for assaying the activity of lysosomal enzymes present in dried bodily fluids and cell tissue samples, such as α-L-iduronidase, β-D-galactosidase, β-D-glucosidase, chitotriosidase, total α-D-galactosidase and α-D-galactosidase A, hexosaminidase A and B, α-D-mannosidase, β-D-mannosidase, α-L-fucosidase, N-acetyl-α-galactosaminidase, arylsulfatases, sphingomyelinase, β-galactocerebrosidase, iduronate-12-31-2009
20110165605METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME - An object is to provide a novel method of improving an enzyme capable of deamidating a protein. A mutant enzyme is designed by the following steps: (1) specifying one or more amino acids selected from the following group, namely, consisting of an amino acid corresponding to the amino acid at position 35, an amino acid corresponding to the amino acid at position 38, an amino acid corresponding to the amino acid at position 39, an amino acid corresponding to the amino acid at position 40, an amino acid corresponding to the amino acid at position 41, an amino acid corresponding to the amino acid at position 42, an amino acid corresponding to the amino acid at position 43, an amino acid corresponding to the amino acid at position 45, an amino acid corresponding to the amino acid at position 46, an amino acid corresponding to the amino acid at position 49, an amino acid corresponding to the amino acid at position 79, an amino acid corresponding to the amino acid at position 80, an amino acid corresponding to the amino acid at position 81, an amino acid corresponding to the amino acid at position 82, an amino acid corresponding to the amino acid at position 83, an amino acid corresponding to the amino acid at position 84, an amino acid corresponding to the amino acid at position 103, an amino acid corresponding to the amino acid at position 104, an amino acid corresponding to the amino acid at position 105, an amino acid corresponding to the amino acid at position 106, an amino acid corresponding to the amino acid at position 117, an amino acid corresponding to the amino acid at position 142, an amino acid corresponding to the amino acid at position 143, an amino acid corresponding to the amino acid at position 146, an amino acid corresponding to the amino acid at position 166, and an amino acid corresponding to the amino acid at position 185 in an amino acid sequence set forth in SEQ ID NO: 2, in a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having substitution of the amino acid(s) specified in the step (1) by another amino acid(s) or having deletion of the amino acid(s) specified in the step (1) using the amino acid sequence for an enzyme to be mutated as a base sequence.07-07-2011
20090298107METHODS FOR DIAGNOSING CHRONIC DIARRHEA THROUGH PROTEIN SECRETION ANALYSIS - Methods for diagnosing chronic diarrhea and other gastrointestinal conditions. In the methods, a sample of gastrointestinal secretions is obtained from a control group; or a group who has been diagnosed with either healthy gastrointestinal tracts or with a gastrointestinal condition, like chronic diarrhea. The control group samples are analyzed in any suitable manner to determine the levels of gastrointestinal secretions, including one or more cytokeratin I subtypes, cytokeratin II subtypes, antimicrobial proteins, mitochondria, and digestive enzymes. The results of the sample analysis is used to create a database containing profiles of normal and abnormal gastrointestinal secretions. As the database is created and specific secretion level abnormalities are identified, patients may be diagnosed with these abnormalities and be treated by adjusting the levels of specific secretions. Gastrointestinal samples from subsequent patients may be analyzed and compared with the database to determine which of the patients' secretion levels, if any, are abnormal.12-03-2009
20110091920METHOD OF DIAGNOSING A CLINICAL CONDITION BY DETECTION OF A PAPP-A/proMBP COMPLEX - The present invention provides a method of diagnosing Down's syndrome, acute coronary syndrome or pre-eclampsia, or a predisposition to any of them, by a method comprising measuring the level of the human PAPP-A/proMBP complex in a body fluid sample.04-21-2011
20120252049METHODS OF DETECTING MICROORGANISMS AND KITS THEREFORE - A method of detecting microorganisms in a test sample is provided. The method includes the steps of: a) incubating the test sample with a growth media to form an incubated sample, wherein the growth media includes an enzyme substrate and the enzyme substrate includes an enzymatically hydrolysable group and a fluorescent group, wherein microorganisms present in the test sample include an enzyme that hydrolyzes the hydrolysable group from the fluorescent group to form a fluorescently detectable product, wherein the fluorescently detectable product has both an acidic and basic species; b) exciting the fluorescently detectable product with light having a wavelength of Exλiso for a time sufficient for the fluorescently detectable product to emit light, wherein Exλiso is the absorbance isosbestic point of the fluorescently detectable product; and c) detecting light emitted at a wavelength of Emλ1.10-04-2012
20120252048COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A CONTRAST AGENT AND METHODS OF USE - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.10-04-2012
20110104727ASSAYS FOR DIAGNOSING AND EVALUATING TREATMENT OPTIONS FOR FABRY DISEASE - Provided are in vitro and in vivo methods for determining whether a patient with Fabry disease will respond to treatment with a specific pharmacological chaperone.05-05-2011
20120122132Protein Structural Biomarkers to Guide Targeted Chemotherapies - A rapid, infrared spectroscopic method has been developed to assess the efficacy of targeted chemotherapeutics against the structure of the polypeptide target, based on the effect of natural polymorphic sequence variation on the conformation of the protein. This method has an advantage over the current genomics-based screening, as the new method provides a direct readout of the structural, and hence functional, outcome of polymorphisms to the protein region targeted by drugs. It allows rapid measurement of a protein's susceptibility to therapeutic targeted agents, prior to using the drug as treatment in the patient. This method can be used to identify biomarkers for a response for a protein to a drug which can be readily tested, interpreted, and used in a clinical setting.05-17-2012
20120122133NOVEL DEVICES FOR THE DETECTION OF THE PRESENCE AND/OR ACTIVITY OF PROTEASES IN BIOLOGICAL SAMPLES - The subject invention provides novel devices and methods for the detection of the presence and/or activity of proteases in biological samples05-17-2012
20090130698CUSTOMIZABLE AND RENEWABLE NANOSTRUCTURED INTERFACE FOR BIOELECTRONIC APPLICATIONS - A chemical composite useful for preparing a bioelectronic device includes a biologically active compound, such as an enzyme, that is bound directly or indirectly to a polyelectrolye, which can be reversibly coupled to a chemically treated electrically conductive substrate by electrostatic forces to provide biomimetic sensors, catalyst systems, and other devices having an electrode that can be regenerated and reused. Required or desired cofactors, mediators or the like may be incorporated into the devices, typically by bonding them to the treated substrate and/or the polyelectrolyte.05-21-2009
20090130697Biological systems input-output response system and plant sentinels - A eukaryotic input circuit: computationally designed receptors, synthetic eukaryotic signal transduction pathways, and a synthetic signal sensitive promoter that allow highly specific transcriptional induction in response to an externally provided ligand is disclosed. The input circuit is able to specifically bind a targeted substance and transmit a signal to the nucleus where transcription of a gene is activated. An output circuit serves as a simple readout system of the substance detected by the input circuit. The readout circuit exemplified here is a degreening circuit which causes plants to turn white. Activation of the degreening circuit can be detected by eye, or remotely with a variety of machines (hand-held, aircraft or satellite based) and is also resettable. When linked the input circuit if operably linked to the output circuit, produces a functional plant detector.05-21-2009
20100209952Methods For Diagnosis And Monitoring Of Neurological Disease By Detection Of An Excephalotoxin - Encephalotoxin produced by activated mononuclear phagocytes is present in individuals having neurological disease including neurodegenerative and neuro-inflammatory diseases, such as Alzheimer's disease (AD), HIV-1-associated dementia (HAD), Creutzfeldt-Jakob disease, Mild Cognitive Impairment, prion disease, minor cognitive/motor dysfunction, acute stroke, acute trauma, or neuro-AIDS. Biochemical detection of encephalotoxin according to the methods of the invention will allow diagnosis of neurological disease in early, presymptomatic stages, thereby allowing early intervention in disease progression as well as identification of subjects or populations at risk for developing neurodegenerative disease. The methods of the invention also provide a mechanism for monitoring progression and treatment of neurological disease.08-19-2010
20100209951METHODS FOR ASSAYING ALPHA-L-IDURONIDASE ENZYMATIC ACTIVITY - Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.08-19-2010
20120135438DETECTING A MICROORGANISM STRAIN IN A LIQUID SAMPLE - The invention concerns a medium for detecting, identifying and differentiating a microorganism strain in a liquid medium by contacting said liquid sample with a combination of chromogens substrates of enzymes expressed or not by the strain to be detected, the final coloration of the mixture being detectable in the wavelengths of the visible light.05-31-2012
20120135437BIOSENSORS UTILIZING INK JET-PRINTED BIOMOLECULE COMPATIBLE SOL GEL INKS AND USES THEREOF - Novel solid-phase biosensors that utilize ink jet printing of biocompatible sol-gel based inks to create sensor strips are reported herein. Biomolecules and other reagents useful in bioassays to detect, for example, pathogenic microorganisms or toxic substances, are immobilized on a substrate, which can be paper based, by layering these substances between two layers of biomolecule compatible sol gel. The sol gel precursor solutions and solutions of the assay reagents are printed from separate nozzles in a layered approach which avoids clogging of the nozzles by the pre-mature gelling of the sol gel precursor solution. In certain embodiments of the application, a capture agent is used to concentrate a compound to be detected in specific areas on the substrate to facilitate detection.05-31-2012
20120135439METHOD FOR PRODUCTION OF ACCUMULATED PRODUCT OF NANO-SUBSTANCE, ACCUMULATED PRODUCT OF NANO-SUBSTANCE, DEVICE COMPRISING THE ACCUMULATED PRODUCT, AND METHOD FOR ANALYSIS OF STRUCTURE OF NANO-SUBSTANCE - A method for producing an accumulated product of a nano-substance that enables the accumulated product of the nano-substance to be produced at low cost, by a simple process that requires few conditions to be controlled and requires minimal energy, and with good reproducibility. Specifically, a method for producing an accumulated product of a nano-substance, the method including crystallizing a protein in a state where the protein and the nano-substance co-exist within a solvent, thereby accumulating the nano-substance within pores of the protein crystals to obtain the accumulated product of the nano-substance.05-31-2012
20120077216Beta-Glucosidase Variants - The invention relates to recombinantly produced β-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant β-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target β-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.03-29-2012
20120077215METHODS AND COMPOSITIONS FOR THE DETECTION OF BETA-LACTAMASES - Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.03-29-2012
20100285512FUNCTIONALIZED BIOCHIPS FOR SPR-MS COUPLING - The invention relates to a method for coupling in-line the analysis of molecular interactions by surface plasmon resonance (SPR) with a structural identification by mass spectrometry using the same functionalized support for both types of analysis.11-11-2010
20100003708LIPOLYTIC ENZYME VARIANTS - Lipolytic enzyme variants with increased specificity for short-chain fatty acids can be designed on the basis of a three-dimensional model of a lipolytic enzyme such as 01-07-2010
20120083010METHOD FOR DIAGNOSING POMPE DISEASE - Provided is a method for diagnosing Pompe disease in a patient by measuring acid α-glucosidase activity in a sample from the patient. The invention also provides a method for monitoring the treatment of Pompe disease with specific pharmacological chaperones by measuring acid α-glucosidase activity in a sample from the patient.04-05-2012
20080299595Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins - The present disclosure relates to tailored glycoproteomic methods, and more particularly to methods for the sequencing, mapping and identification of cellular glycoproteins using saccharide-selective bioorthogonal probes. A method is disclosed for saccharide-selective glycoprotein identification (ID) and glycan mapping (GIDmap) that generates glycoproteins tailored with bioorthogonally tagged alkynyl saccharides that can be selectively isolated, allowing for glycoprotein ID and glycan mapping via mass spectromic proteomics, including liquid chromatography-tandmen mass spectroscopy (LC-MS12-04-2008
20120270252MEDIUM FOR THE SPECIFIC DETECTION OF RESISTANT MICROORGANISMS - A method for distinguishing among a first group of microorganisms belonging to a first taxon of Gram negative bacteria, the first group of bacteria exhibiting a mechanism of resistance to a treatment; a second group of microorganisms belonging to a second taxon of Gram negative bacteria, the second taxon of bacteria being different than said first taxon, and exhibiting a mechanism of resistance to a treatment identical to the mechanism of the first group; and a third group of Gram negative bacteria that is not resistant to the treatment.10-25-2012
20100203564Fluorescent Phospholipase A2 Indicators - Compositions, methods of synthesis and applications of phospholipase A08-12-2010
20110236916METHODS AND MATERIALS FOR DETECTING COLORECTAL NEOPLASM - The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals having a colorectal neoplasm by detecting the presence of exfoliated epithelial markers (e.g., human DNA, tumor associated gene alterations, tumor associated proteins) and blood markers (e.g., homoglobin, serum proteins) in a stool sample obtained from the mammal.09-29-2011
20100233744Enzyme substrates for visualizing acidic organelles - The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.09-16-2010
20120276568DRY TEST STRIP AND METHOD FOR MEASURING CREATININE - A dry test strip for measuring creatinine comprises: a support; a reagent layer that is disposed on the support; a reagent holding layer that is disposed on the reagent layer; and a connection layer that is composed of an adhesive which adhesively bonds the reagent layer to the reagent holding layer and is formed in spot form, wherein the reagent layer contains creatininase and 4-aminoantipyrine; the reagent holding layer contains creatinase, sarcosine oxidase, peroxidase, and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline; and the connection layer delays arrival of a liquid sample spot-deposited on the reagent holding layer at the reagent layer.11-01-2012
20100129843CHARACTERIZATION OF N-GLYCANS USING EXOGLYCOSIDASES - The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.05-27-2010
20100129842ISOBARICALLY LABELED ANALYTES AND FRAGMENT IONS DERIVED THEREFROM - This invention pertains to isobarically labeled analytes and fragment ions thereof.05-27-2010
20130017565METHODS AND PRODUCTS RELATED TO LOW MOLECULAR WEIGHT HEPARIN - The invention relates to methods and products for characterizing and using polysaccharides. Low molecular weight heparin products and methods of use are described. Methods for characterizing purity and activity of polysaccharide preparations including glycosaminoglycans such as heparin are also described.01-17-2013
20080248513Methods for detection of lysosomal storage disease - The present invention provides compositions for performing assays of enzyme activity associated with lysosomal storage diseases. The invention further provides methods for determining enzyme activity, and methods for the screening for lysosomal storage disease in an individual.10-09-2008
20080227133KpnI Restriction Endonucleases with Reduced Star Activity - Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.09-18-2008
20080227132Hydrolase Enzymes and Their Use in Kinetic Resolution - The invention relates to hydrolases and to polypeptides having hydrolase activity. In addition, the invention relates to the use of these hydrolase enzymes in kinetic resolution.09-18-2008
20130130292Method for detecting replication or colonization of a biological therapeutic - Methods for detecting replication in or colonization of a host by a biological therapeutic, such as an oncolytic virus, cells administered for cell therapy and gene therapy vectors, are provided. In the methods, a product produced by the biological therapeutic is detected in a sample of tissue or body fluid distinct from the administered therapy or locus thereof, thereby permitting assessment of the therapy and/or monitoring its progress.05-23-2013
20130143249METHOD FOR DETERMINING PECTIN CONTENT IN PLANT SAMPLE - Disclosed is a method for determining pectin content in a plant sample, comprising the following steps: 1) adding an acidic alcohol solution to the plant sample, then heating the resulting mixture to reflux in a water bath, followed by a first filtration; 2) soaking the filtered residue obtained from the first filtration with an acidic solution, then heating the resulting mixture to reflux in a water bath, followed by a second filtration, then bringing to volume after cooling, obtaining filtrate for later use; 3) adding an acetic acid/sodium acetate buffer solution to treat the filtered residue obtained from the second filtration, then adding a pectinase solution and heating the resulting mixture under vibration in a water bath, followed by a third filtration to obtain a filtrate for later use; 4) sequentially adding an acetic acid/sodium acetate buffer solution and a pectinase solution to the filtrate obtained in step 2) and heating the resulting mixture under vibration in a water bath to obtain an enzymatic hydrolysate, then adding the filtrate obtained in step 3) to the enzymatic hydrolysate followed by bringing to volume, obtaining a test solution; and 5) drawing the test solution into a continuous flow analyzer to perform analysis. The method disclosed has the advantage of providing accurate analysis results.06-06-2013
20130143248Force-Clamp Spectrometer And Methods Of Use - The disclosed subject matter relates to a force-clamp spectrometer that enables operation in constant force mode and allows for automated data acquisition and analysis, using feedback electronics and software. The disclosed subject matter also relates to methods of using the force-clamp spectrometer for the measurement of the dynamics of chemical reactions. The methods may include, but are not limited to, the measurement of the dynamics of substrate folding and unfolding, as well as bond cleavage and bond formation.06-06-2013
20130095511MASS SPECTROMETRIC MEASUREMENT OF B-LACTAMASE RESISTANCES - The invention relates to the determination of resistances of microorganisms which produce β-lactamases, in particular “extended spectrum β-lactamases” (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced β-lactamases on β-lactam antibiotics, which consists in a hydrolytic cleavage of the β-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a β-lactam antibiotic or a customized β-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the β-lactamases.04-18-2013
20130115643Colorimetric Device for Detecting, in an Aqueous Solution of Interest, Hydrolytic Enzymatic Activity with Regard to at Least One Polymer of Interest - This invention concerns a colorimetric device for detecting, in an aqueous solution of interest, a hydrolytic enzymatic activity with regard to at least one polymer of interest.05-09-2013
20110212476GENETICALLY ENGINEERED G-ALPHA PROTEINS AND USES THEREOF - The present invention relates to novel engineered Ga proteins and assay methods of using such proteins to advance drug discovery. Engineered Ga proteins described by the invention contain alterations of at least one and preferably two or more amino acid residues that are highly conserved among all four subfamilies of Ga proteins. A preferred engineered protein disclosed here is a double mutant, Gαπ R178M A326S. This specific combination of mutations yields an unexpectedly amplified effect on Ga function both in terms of GTPase activity (GTP hydrolysis) and GDP dissociation. This synergistic effect may have a profound influence on the way GPCR signaling pathways are examined for the development of new pharmacotherapies, particularly in the field of central nervous system disorders such as Parkinson's disease.09-01-2011
20080199896Diagnosis and Treatment of Diseases Arising from Defects in the Tuberous Sclerosis Pathway - The present invention relates to compositions and methods for identifying abnormalities in TSC signaling pathways. In particular, the present invention relates to methods of diagnosing and treating disorders such as tuberous sclerosis, which are caused by mutations in the TSC genes. The present invention further relates to methods and compositions for treating cancers mediated by TSC signaling disorders.08-21-2008
20080199895Highly purified and stabilized Na,K-ATPase isoforms and methods of producing same - A composition of matter comprising active Na,K-ATPase is disclosed, each Na,K-ATPase comprising an α and β subunit, the Na,K-ATPase being at least 85% purified. Methods of purifying same and uses thereof are also disclosed.08-21-2008
20130137126USE OF A BETA-GLUCOSIDASE ACTIVATOR FOR THE DETECTION AND/OR IDENTIFICATION OF C. DIFFICILE - The present invention relates to a reaction medium comprising at least one beta-glucosidase substrate and a compound of the general formula Ar-beta-D-glucoside where Ar- designates an aromatic compound, different from said substrate. According to the invention, such a medium can be employed in a 05-30-2013
20090093007METHOD FOR PRODUCING DRY ANALYTICAL ELEMENT FOR PANCREATIC LIPASE MEASUREMENT - It is an object of the present invention to provide a method for producing a dry analytical element for pancreatic lipase analysis in a stable manner with good accuracy, such element having high specificity with respect to pancreatic lipase and being available for convenient use. The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid, said element comprising at least one development layer or reagent layer containing diglyceride or triglyceride of long-chain alkyl fatty acid having 14 to 20 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent which comprises a step of adding glyceride dissolved in lower alcohol or acetone to the development layer or reagent layer and drying the glyceride.04-09-2009
20100291608NOVEL GTP CYCLOHYDROLASE TYPE IB - This invention relates to a novel, bacterial GTP Cyclohydrolase Type IB enzyme, and the crystal structure thereof.11-18-2010
20130102017FLUORESCENT CARBAPENEMS - Chromogenic or fluorescent carbapenems according to formula I, wherein Ar is a mono or disubstituted carbocyclic aromatic group or an optionally mono or disubstituted heterocyclic aromatic group, are useful compounds for the detection of bacterial carbapenemase.04-25-2013
20100317043MASS SPECTROMETRY ASSAY FOR eIF4E AND eIF4E REGULON ACTIVITY - Provided is a highly sensitive high throughput mass spectrometry-based quantitative assay for 4E/4E regulon pathway proteins has been developed which provides for single sample multiplexed analysis, as well as the analysis of protein phosphorylation states. It may be adapted for use as the first single sample analytical method of the 4E/4E regulon biological pathway.12-16-2010
20100317042PROTEINS ACTIVATING PRO-PHENOLOXIDASE SYSTEM AND GENES ENCODING THE SAME - The present invention provides novel proteins activating pro-phenoloxidase (pro-PO) system of 12-16-2010
20130183700SULFONATED COUMARINS, SYNTHESIS THEREOF, FLUOROGENIC SUBSTRATES RESULTING FROM GRAFTING SAID COUMARINS ONTO SUGARS, METHOD FOR PREPARING SAID SUBSTRATES, AND USES THEREOF - Substituted sulfonated coumarins are expressed in the general formula (I), where: R07-18-2013
20110312007Detection of Histone Deacetylase Inhibition - Provided are compositions and methods for intracellular detection of enzyme activity. One example of a composition is a histone deacetylase substrate comprising a compound of the following formula (I):12-22-2011
20110312006Enzymatic Assay for the Quantitative Determination of Phospholipase A1 or A2 Activity in a Sample - The invention relates to a new method for measuring a calcium-dependent phospholipase A1 or A2 enzymatic activity in a sample, comprising the following steps: a) contacting said sample with a solid phase coated with a fluorochrome-labelled phospholipase A1 or A2 substrate, wherein the molecular coverage is in the range 8 to 30 fluorochrome-labelled phospholipase A1 or A2 substrate molecules/nm2 in conditions where calcium-dependent phospholipase A1 or A2 enzymatic activity is blocked. b) reading the fluorescence emission overtime c) adding a solution to initiate calcium-dependent phospholipase A1 or A2 enzymatic activity d) reading the fluorescence emission overtime, and kits for carrying out said method.12-22-2011
20130210047SILICA NANOPARTICLES WITH AGGREGATION INDUCED EMISSION CHARACTERISTICS AS FLUORESCENT BIOPROBE FOR INTRACELLULAR IMAGING AND PROTEIN CARRIER - Provided herein are magnetic silica fluorescent nanoparticles and fluorescent silica nanoparticles comprising an aggregation induced emission luminogen and magnetite nanoparticles and use of the same as a fluorescent bioprobe for intracellular imaging and a protein carrier. Also provided are processes for preparing and fabricating the same.08-15-2013
20130210048METHOD OF DETECTING A BIOLOGICAL ACTIVITY - A method of detecting a biological activity is provided. The method includes contacting; in a liquid medium selected to facilitate a predetermined biological activity; a sample, an indicator reagent, and a substrate that receives and concentrates a biological derivative of the indicator reagent. The method further includes observing a portion of the substrate to detect the biological derivative. The method can be used to detect the presence or absence of a target cell.08-15-2013

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