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MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT

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435 - Chemistry: molecular biology and microbiology

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DocumentTitleDate
20090220932DEVICE AND METHOD FOR COMBINED MICROFLUIDIC-MICROMAGNETIC SEPARATION OF MATERIAL IN CONTINUOUS FLOW - A miniaturized, integrated, microfluidic device pulls materials bound to magnetic particles from one laminar flow path to another by applying a local magnetic field gradient. The device removes microbial and mammalian cells from flowing biological fluids without any wash steps. A microfabricated high-gradient magnetic field concentrator (HGMC) is integrated at one side of a microfluidic channel. When magnetic particles are introduced into one flow path, they remain limited to that flow path. When the HGMC is magnetized, the magnetic beads are pulled from the initial flow path into the collection stream, thereby cleansing the fluid. The microdevice allows large numbers of beads and materials to be sorted simultaneously, has no capacity limit, does not lose separation efficiency as particles are removed, and is useful for cell separations from blood and other biological fluids. This on-chip separator allows cell separations to be performed in the field outside of hospitals and laboratories.09-03-2009
20090246750Devices and Methods for Collection, Storage and Transportation of Biological Specimens - The present invention provides methods for collecting, storing or transporting liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released for subsequent analysis by compressing or centrifuging the matrix. Also provided are method of using kits for collecting, storing, transporting and recovering biological specimens containing analytes of interest.10-01-2009
20120183947MULTIPLE LAMINAR FLOW-BASED PARTICLE AND CELLULAR SEPARATION WITH LASER STEERING - The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.07-19-2012
20120183946Fabrication of Microfilters and Nanofilters and Their Applications - Micro- and nanofilters with precision pore sizes and pore layout have applications in many fields including capturing circulating tumor cells and fetal cells in blood, water treatment, pathogen detection in water, etc. Methods to fabricate micro- and nanofilters not using track etching or reactive ion etching are provided, allowing easy fabrication of single layer or stack of films simultaneously, and/or stack of films on rolls. Microfilter can be made using one or more layers of material. Invention enables mass production of microfilters with lithographic quality at low cost. Isolation, enumeration and characterization of circulating tumor cells using microfilters provides (i) guides to cancer treatment selection and personalize dosage, (ii) low cost monitoring for treatment response, disease progression and recurrence, (iii) assessment of pharmacodynamic effects, (iv) information on mechanisms of resistance to therapy, and (v) cancer staging. Microfabrication methods are also applicable to fabrication of any free standing patterned polymeric films.07-19-2012
20090191537MEDIUM AND METHODS FOR THE STORAGE OF PLATELETS - Synthetic storage media are disclosed for use in the processing and the storing of platelets. The storage media includes a platelet storage solution and less than 20 percent plasma to preserve platelet function after at least 7 days of storage.07-30-2009
20100151438METHODS AND KITS FOR ENHANCING SEDIMENTATION AND RECOVERY OF CELLS IN A SAMPLE - The methods and kits provide sedimentation-enhancing agents that significantly increase the efficiency of cell-sedimentation. They also increase the efficiency of blood cell separation methods and thereby increase the recovery of total nucleated cells.06-17-2010
20130078608ZEODRATION METHOD FOR THE PRESERVATION OF BLOOD PLATELETS - The present invention relates to a new method for the preservation of blood platelets. In particular, the present invention provides a method which includes submitting the platelets to zeodration carried out at temperatures between 18° C. and 24° C. The dried platelets thus obtained can be preserved at room temperature for long period of time. The invention also provides kits and compositions for using the preserved platelets.03-28-2013
20130084558SPERM STAINING AND SORTING METHODS - A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.04-04-2013
20130034841SYSTEMS AND METHODS FOR ISOLATING AND CHARACTERIZING TARGET MATERIALS OF A SUSPENSION - Systems and methods for isolating and characterizing various target materials of a suspension are disclosed. A suspension suspected of containing the target materials is added to a tube. A float with a specific gravity corresponding to that of the target material is inserted into the tube. The tube, float, and suspension are centrifuged together causing the various materials suspended in the suspension to separate into different layers along the axial length of the tube according to their specific gravities. The float and/or tube are configured to drive the various target materials to a region of space between the float and inner wall of the tube.02-07-2013
20130040282DEVICE AND METHOD FOR REMOVING ADDITIVES IN THE BLOOD PRODUCTS - A method for removing a photosensitizer from a platelet preservation composition is disclosed. The method comprises passing the platelet preservation composition through a tangential flow filtration device to separate the photosensitizer from the platelets in the platelet preservation composition.02-14-2013
20100099075SEMEN MANIPULATION PROCESS - The present invention refers to a semen manipulation process that aims at separating spermatic cells from their maintenance medium. The process the invention deals with allows for an increase in the final concentration of the obtained sperms and for an increase in the production indexes of in vitro embryos, presenting a great application in the assisted reproduction area of humans and animals overall. The invention describes a filtering system for the separation of spermatic cells from their maintenance medium characterized by the fact that it is made up by a filtering membrane that remains in contact with a primary plate placed on the heating plate and that also keeps the temperature constant throughout the process. The procedure includes filtering by using flush solutions and final maintenance solutions that are selected according to the later application of separated spermatic cells. Sperms withheld in the filtering membrane are resuspended and they may be used for artificial insemination, freezing, in vitro fecundation or sexying of the X and Y sperms in the flow cyto meter.04-22-2010
20090155764WHITE BLOOD CELL DIFFERENTIATION REAGENT AND METHOD - A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolarity below 50 mOsm/kg H06-18-2009
20090155763COMPOSITIONS AND METHODS FOR PROLONGING SURVIVAL OF PLATELETS - The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.06-18-2009
20100105020QUANTITATIVE MEASUREMENT OF GLYCATED HEMOGLOBIN - The invention relates to a method for quantitative measurement of glycated hemoglobin (HbAIc) in a blood sample containing hemoglobin molecules. In this method, the hemoglobin molecules are extracted from the blood sample, adsorbed onto a roughened metal surface and subjected to a Surface Enhanced Raman Scattering (SERS) measurement. The invention provides a universal method for the measurement of the HbAIc concentration in blood.04-29-2010
20100041011Reference Control for Cell by Cell Analysis - A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.02-18-2010
20100041012Rationally designed antibodies - Antibodies or fragments thereof having CDR regions replaced or fused with biologically active peptides are described. Flanking sequences may optionally be attached at one or both the carboxy-terminal and amino-terminal ends of the peptide in covalent association with adjacent framework regions. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.02-18-2010
20100105021STABILISATION OF BIOLOGICAL CELL MARKERS - The invention provides compositions which stabilise the expression of cell surface markers. The invention further provides methods and kits comprising such compositions for use in stabilising the expression of cell surface markers. The invention is especially concerned with compositions for use in stabilising the expression of cell surface markers (such as cell activation markers) present on blood cells, such as platelets. The invention extends to methods, apparatus and kits for detecting platelet activation, and to kits for monitoring the efficacy of anti-thrombotic treatment regimes.04-29-2010
20130029313APPARATUS AND METHOD FOR SEPARATING COMPONENTS - An apparatus for separating components and a method of separating components using the apparatus are provided. The apparatus includes: a main chamber which contains a sample that is separated into a plurality of layers by a centrifugal force; a component separating chamber which is connected to the main chamber, and receives a specific layer including specific components among the plurality of layers; a first channel which connects the component separating chamber to the main chamber; and a first channel valve which is disposed in the first channel to control a liquid flowing through the first channel.01-31-2013
20130029312INTEGRAL SPERM PREPARATION FOR INTRACYTOPLASMIC SPERM INJECTION - Methods, systems and apparatus for preparing sperm for ICSI by combining selective enrichment benefits of sperm gradient preparation, sperm swim-up preparation and hyaluronan-binding for selection of mature sperm into a single processing unit. The single processing unit of the invention reduces operator labor steps and time, is efficient, easy to use, offers quick processing time and provides highly motile, mature and specific individual sperm that retain desired sperm characteristics selected by each individual selection principal that are ready for capture and use in ICSI.01-31-2013
20090123907LYSINE CITRATE FOR PLASMA PROTEIN AND DONOR PROTECTION - An improved anticoagulant or additive is based on a higher level of citric acid than is usual (at least about 1.0% weight by volume). The higher citrate is combined with an amino acid as a counterion. The amino acid prevents cellular damage often caused by elevated citrate levels. The amino acid citrate mixture also serves to preserve platelet concentrates and platelet rich plasma during room incubation. Not only does the amino acid citrate combination enhance platelet integrity, it completely inhibits or kills bacteria such as 05-14-2009
20090123906Reproductive cell system - Compositions or extracts (05-14-2009
20090202979Production of antibody formats and immunological applications of said formats - The invention concerns an in-vitro method for introducing a targeted genome modification into an oocyte or an egg and a method for performing a random insertion in the genome of a host cell.08-13-2009
20100003663Composition For Preserving Reproductive Cells And Method Of Using - Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian or avian reproductive sperm cells. The compositions comprise suitable reproductive cell media and antioxidant bioflavonoids. These may be comprised of Oligomeric Proanthocyanidins (OPC) which include the specific molecules of OPCs, that are namely; (epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)).01-07-2010
20090317790Method for the Production of Frozen Blood or Frozen Blood Cells for Biological Assays - The present invention relates to an improved method for cryo-preservation of human whole blood containing monocytes and lymphocytes at temperatures of between −40° C. and −60° C., preferably at −60° C., whereby the function of monocytes and lymphocytes is preserved. The present invention, particularly relates to a method for cryo-preservation of human whole blood containing monocytes and lymphocytes without the need for an isolation of peripheral blood mononuclear cells (PBMCs), or blood cells.12-24-2009
20090305224Methods for the Cryopreservation of Mammalian Cells - The present invention features novel methods for the cryopreservation of mammalian cell that combine the advantages of the slow-freezing and vitrification approaches while avoiding their shortcomings. Generally, the methods include the use of a capillary tube made of a thermally conductive wall material and a thin wall such that the ratio of the thermal conductivity of the wall material to the wall thickness is at least 1,000-500,000. The solution is then exposed to temperatures equal to or less than −80° C. and the vitrification solution containing the mammalian cells is cooled at a rate equal to or greater than 30,000-100,000,000° C./minute. The exposure of the capillary tube with a thermally conductive and thin wall allows for vitrification of the solution in the absence of ice formation. Cryoprotectants can also be added to the vitrification solution to further prevent ice formation.12-10-2009
20090092958Method of Inactivating Blood Coagulation Factor and Blood Coagulation Factor-Inactivated Sample - A method of inactivating a blood coagulation factor is described. The method comprises a step of contacting a sample containing at least one of factors V and VIII with a compound having an iminodiacetate group, whereby at least one of factors V and VIII in the sample is changed into an inactivated form.04-09-2009
20130059288METHOD FOR ISOLATING TARGET CELLS - The present invention relates to a method for the enrichment and/or isolation of target cells in a sample, which sample comprises red blood cells and/or platelets, comprising (a) filtering the sample through a filter element having a pore or mesh size of between 0.5 and 5 μm, (b) contacting the cells retained by the filter element in step (a) with a separation surface, wherein said separation surface comprises affinity molecules which selectively bind to the target cells, (c) incubating the cells and the separation surface under conditions which allow for the binding of the affinity molecules to the target cells; and (d) separating the separation surface from any unbound cells and material.03-07-2013
20130059287Prolonging Survival of Platelets Using CMP-Sialic Acid, UDP-Galactose or Both - The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.03-07-2013
20120308985Immature Reticulocyte Fraction Reference Control and Related Methods - A composition (and associated methods) including a plurality of treated red blood cells for simulating reticulocytes, and particularly an immature reticulocyte fraction, of whole blood when processed as a sample in an automated analyzer capable of detecting reticulocytes. A method for making the composition or other simulated reticulocyte may include steps of contacting a suspension of a plurality of red blood cells each having a membrane in an initial state that surrounds an interior volume of a cell with an effective amount of a hypertonic permeabilizing solution including dimethyl sulfoxide and a hypotonic loading agent delivery solution including a loading agent, for a sufficient time to form a plurality of pores in the membrane, for permitting the loading agent to enter into the interior volume of the cells.12-06-2012
20090280470EXTRACTION AND DIAGNOSTIC FLUID DEVICES, SYSTEMS AND METHODS OF USE - The present invention provides devices, systems and methods for RNA isolation from biological samples containing white blood cells, such as whole blood. The devices have a device body that includes a first chamber having a first membrane that selectively binds white blood cells, a second chamber having a second membrane that reversibly binds RNA and a plurality of ports that are removably attached to a reagent pack and waste receptacle via a valving system controlled by a control unit.11-12-2009
20120225417Methods, Compositions and Kits for the Evaluation and Preservation of Sperm - The invention concerns methods and kits for evaluating the semen quality. The invention also concerns compounds and compositions for preserving a sperm sample, especially during cryopreservation. The invention is based on a correlation between sperm phosphodiesterase (PDE) activity and sperm fertility and on the use of selective phosphodiesterase inhibitors, more particularly PDE10 inhibitors.09-06-2012
20120225416METHOD FOR ENHANCING RED BLOOD CELL QUALITY AND SURVIVAL DURING STORAGE - The present invention is a method for enhancing the quality and survival of red blood cells during storage by depleting the red blood cells of both carbon dioxide and oxygen and maintaining 2,3-diphosphoglycerate acid levels.09-06-2012
20090258338SIMULATED 5-PART DIFFERENTIAL LEUKOCYTE GRANULES, THE METHOD FOR PREPARING THE SAME AND CONTROL PRODUCTS AND CALIBRATORS COMPRISING THE SAME - A method for preparing five-part differential leukocyte analogs from red blood cells includes selecting appropriate red blood cells; treating the red blood cells with a multi-functional reagent system for maintenance of a structural integrity of the membranes of the red blood cells and synchronous adjustment of cell volume, morphology and inclusions; subjecting the treated red blood cells to an intensified fixation; and washing the treated cells for preservation. The present disclosure also relates to leukocyte analogs prepared by the above method, a reagent system used for preparing the analogs, and a quality control and a calibrator including the analogs for use in a hematology analyzer.10-15-2009
20090053686Particle Switching Systems and Methods Using Acoustic Radiation Pressure - The present invention comprises methods and systems that use acoustic radiation pressure.02-26-2009
20120115124IRRADIATION OF RED BLOOD CELLS AND ANAEROBIC STORAGE - A blood storage system comprising: a collection vessel for red blood cells; an oxygen or oxygen and carbon dioxide depletion device; a storage vessel for red blood cells; tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device and the oxygen or oxygen and carbon dioxide depletion device to the storage vessel; and a gamma or X-ray irradiating device is used to irradiate red blood cells stored in the vessel, storing red blood cells under anaerobic conditions.05-10-2012
20130164732INORGANIC PYROPHOSPHATE AND USES THEREOF - The present invention provides a new and improved sperm stimulating additive comprising a certain amount of inorganic pyrophosphate (PPi). Addition of PPi in the media for human/animal in vitro fertilization (IVF) improves fertilization rate; addition of PPi in the semen extender for farm animal artificial insemination (AI) may improve pregnancy rates; furthermore, mammalian oocytes matured in vitro in a medium including PPi attain improved fertilization and developmental potential, while embryos cultured in medium supplemented with PPi have improved development to blastocyst.06-27-2013
20090191536Rabbit monoclonal antibodies to hepatitis B surface antigens and methods of using the same - Reagents, methods and immunodiagnostic test kits for the accurate detection of hepatitis B virus (HBV) infection are disclosed. The methods and kits employ novel rabbit monoclonal antibodies directed against HBV surface antigens (HBsAg) with mutations in the “a” determinant region of HBsAg.07-30-2009
20090155762Device and Method for Separating and Discharging Plasma - The invention concerns plasma separation on a microliter scale. The method/system according to the invention is able to provide plasma in the range of several microliters within a very short time as it is required, for example, in modern analyses by carrier-bound test elements. Plasma separation and plasma release is carried out in two separate consecutive steps of the method using the device according to the invention such that hemolysis during plasma separation can be avoided despite an accelerated procedure. The device comprises a disposable unit in which the device is characterized by an economical and simple method of production.06-18-2009
20090029342Medium and method for preserving platelets, red blood cells, and other non-nucleus cells and platelets-containing composition - The present invention provides a platelet-containing composition prepared by contacting platelets with a medium for preserving. The medium comprises anticoagulant, cryoprecipitate and thrombin. The present invention also provides a method for long-term preservation of platelets, comprising the steps of: (a) adding an anticoagulant, cryoprecipitate, thrombin in normal saline; (b) adding a platelet-containing medium into the solution formed in step (a); and (c) lyophilizing the platelet-containing solution formed in step (b). Moreover, the present invention yet provides a medium for preserving non-nucleus cells.01-29-2009
20100021879COMPOSITIONS AND METHODS FOR PRESERVING RED BLOOD CELLS - The present invention provides methods, compositions and kits for storing red blood cells for extended periods of time. In particular, the present invention provides methods, compositions and kits for storing red blood cells for extended periods of time while preventing red blood cell storage lesions, retaining red blood cell deformability, and increasing survival of the red blood cells following transfusion.01-28-2010
20100196872Semen extender composition and methods for manufacturing and using - A semen extender composition is provided. The semen extender composition includes a sperm cell protecting amount of phospholipid, an effective amount of surfactant to reduce ice crystal formation during freezing of the composition; a carbohydrate, and a biological buffer to provide a semen extender use solution having a pH of between about 6.9 and about 7.5, and wherein the use solution exhibits an osmolality of about 250 mOsM to about 350 mOsM. Methods for manufacturing and using a semen extender composition are provided.08-05-2010
20100028852METHODS AND REAGENTS FOR PRESERVING RNA IN CELL AND TISSUE SAMPLES - This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue.02-04-2010
20090317789METHOD FOR QUANTIFYING A CELL POPULATION OF INTEREST CONTAINED IN A HUMAN BLOOD SAMPLE - The present invention provides to a method for quantifying a cell population of interest contained in a human blood sample, comprising the steps of: contacting a sample of human blood with a cocktail comprising a first component, comprising at least one non-human antibody, antibody fragment or derivative thereof, capable of specifically binding to an antigen expressed on the surface of a first cell population distinct from a second cell population, said second cell population being the cell population of interest and being devoid of said antigen, complexed to a second component, comprising at least one non-human antibody, antibody fragment or derivative thereof, capable of specifically binding to the surface of human erythrocytes; separating antibody-labelled from unlabelled cells contained in the blood sample; collecting the supernatant comprising the second cell population; concentrating the second cell population contained in the supernatant and resuspending said cell population in a liquid capable of selectively lysing erythrocytes; concentrating the obtained cells, essentially removing all supernatant and resuspending said cells in a defined volume of physiological buffer; and determining the number of the cells of the second cell population contained in the sample. Furthermore, the invention provides to a kit for performing the method of the invention.12-24-2009
20090317788Novel Carbohydrate Profile Compositions From Human Cells and Methods for Analysis and Modification Thereof - The present invention discloses a method of evaluating the status of a stem cell preparation comprising the step of detecting the presence of a glycan structure or a group of glycan structures in said preparation. The detection step can be performed by the use of a lectin specific to a glycan structure of interest.12-24-2009
20130189668BLOOD SEPARATION AND CONCENTRATION SYSTEM - This invention provides an apparatus and methods to consistently separate and concentrate selected blood components. The system includes, e.g., a computerized fluid handling system to transfer blood components between a centrifugal blood separation disc, containers and a concentrator.07-25-2013
20100041010Method to treat collagenous connective tissue for implant remodeled by host cells into living tissue - The invention relates to a method of treatment of collagenous connective tissue removed from a donor for implant into a recipient which is re-habited or re-colonized by host cells without an immune rejection and inflammatory reaction. After removal from the donor the tissue is trimmed and thereafter soaked in a cold stabilizing solution having a temperature range of 4 to 10 degrees centigrade. The tissue is then soaked at a predetermined temperature in a polyglycol, salt, hydrogen peroxide, and phosphate buffer first solution of predetermined quantities and concentrations and of sufficient ionic strength to permit ground substances to dissociate such that the collagen fibers remain stable. The tissue is then soaked in an alcohol and water solution at a predetermined temperature for a sufficient period of time to remove the residue of the first solution. Following the removal of the residue, the tissue is soaked at a predetermined temperature in a third solution of an anti-inflammatory agent, an anti-thrombic agent, alcohol, and water or sequentially in an anti-inflammatory agent, alcohol, and water solution, and then in an anti-thrombic agent, alcohol and water solution and thereafter stored. 02-18-2010
20100099074COLLECTION DEVICE AND METHOD FOR STIMULATING AND STABILIZING A BIOLOGICAL SAMPLE - A collection device and methods for stimulating and stabilizing a biological sample are provided. Aspects of the device include an evacuated sample collection container having stimulating and stabilizing additives contained in separate internal chambers of the container. The internal chambers are arranged to facilitate stimulation upon collection of a sample, followed by stabilization of the sample after a desired period of time in the same sealed container. Also provided are methods for use of the subject device. The device and methods find use in a variety of different applications, including the collection and analysis of clinically relevant samples, such as whole blood and other body fluids, obtained directly from a patient.04-22-2010
20110027771METHODS AND COMPOSITIONS FOR CELL STABILIZATION - Fragile cells have value for use in diagnosing many types of conditions. There is a need for compositions that stabilize fragile cells. The stabilization compositions of the provided invention allow for the stabilization, enrichment, and analysis of fragile cells, including fetal cells, circulating tumor cells, and stem cells.02-03-2011
20130011824Polymeric Sorbent for Removal of Impurities From Whole Blood and Blood Products - The invention concerns methods of treating blood, blood products or physiologic fluid to maximize shelf life and/or minimizing transfusion related complications such as non-hemolytic transfusion reactions such as fever, transfusion-related acute lung injury (TRALI), transfusion associated dyspnea (TAD), and allergic reactions by removing undesirable molecules in the blood, blood product or physiologic fluid milieu through use of a sorbent.01-10-2013
20120141974CRYOPRESERVATION OF HUMAN RED BLOOD CELLS - A red blood cell storage composition includes a composition of red blood cells and biochemistry altering reagents, the biochemistry altering reagents being present at a concentration so as to reduce the percent hemolysis of the red blood cells during the freeze-thaw cycle below that of the percent hemolysis of the red blood cells in the absence the biochemistry altering reagents. The red blood cell storage composition preferably includes reagents selected from: modifiers of glycolytic/metabolic components, modifiers of antioxidant potential, effectors of intracellular ionic distribution, modifiers of membrane fluidity, modifiers of cytoskeletal structure, effectors of the cyclooxygenase second messenger pathway, effectors of the lipoxygenase second messenger pathway, effectors of the hexose monophosphate second messenger pathway, effectors of the phosphorylation second messenger pathway, modifiers of specific messenger molecules, and combinations thereof.06-07-2012
20090298045Method For Selectively Expanding, Selecting And Enriching Stem/Progenitor Cell Populations - A method of producing stem/progenitor cells from human or animal origin. A population, from an embryonic, fetal or adult source, preferably from bone marrow, blood, fat, muscle, heart, intestine, kidney, liver, lung, pancreas, skin or neural tissues, that includes stem/progenitor cells, is treated with one or more first cytostatic or cytotoxic agents to which the stem/progenitor cells are less sensitive than the other cells of the population. Preferably, the agent(s) selectively deplete(s) from the population cells that are negative with respect to expressing a transporter gene of the first agent(s) while sparing cells that are positive with respect to expressing that gene. Preferably, the population also is treated with one or more cytokines and/or growth factors.12-03-2009
20100081121MEDIA AND METHOD FOR CELL SEPARATION - The present invention relates to a separation media and a method for separation of cells from different cell sources, such as umbilical cord blood, using the separation media to obtain the desired cells, such as stem cells. The separation media has beads with a diameter of 200-500 μm and is provided with cell separation ligands. The cell specific ligands are preferably CD3 and CD19 for depletion of B and T cells and production of a stem cell rich product.04-01-2010
20120107791COMPOSITIONS AND METHODS FOR PROLONGING SURVIVAL OF PLATELETS - The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.05-03-2012
20090263781Composition for Preserving Platelets and Method of Using the Same - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state.10-22-2009
20120295247PORCINE SEMINAL MATERIAL COMPOSITION FOR ARTIFICIAL INSEMINATION OF SOWS - A porcine seminal material composition intended for artificial insemination of sows includes capsules containing a suspension made of porcine seminal material coated with a bi-valent or tri-valent ion alginate film, wherein the porcine spermatozoon concentration within the porcine seminal material suspension present in the capsules is between 900 and 1500 million spermatozoa per milliliter.11-22-2012
20080206736FLAVIN N-OXIDES: NEW ANTI-CANCER AGENTS AND PATHOGEN ERADICATION AGENTS - Compounds comprising flavin N-oxides for treatments of solid tumors, non-solid tumor masses, leukemias, and non-small cell lung cancers and for eradicating contaminants in blood products. Methods of treating patients having solid type cancers comprising administering a therapeutically effective amount of a flavin N-oxide to a subject in need of treatment and exposing the flavin N-oxide to an activator such that activation of the flavin N-oxide results in damage to the DNA in the cancer cells without substantial damage to the DNA of normal cells are also provided. Methods of using a flavin N-oxide as part of a combination therapy with chemotherapy, radiation therapy, or both are also provided. Methods of reducing pathogenic bacterial or viral contamination in a composition comprising a) mixing the composition with an efficacious amount of a flavin N-oxide and b) exposing the mixture of the composition and the flavin N-oxide to an activator for a period of time sufficient to activate the flavin N-oxide such that the flavin N-oxide reduces the contamination in the composition are also provided. Preferably, the composition is a blood product selected from plasma, platelets, and red blood cells and the activator is an enzyme.08-28-2008
20090291427INTEGRATION OF SAMPLE STORAGE AND SAMPLE MANAGEMENT FOR LIFE SCIENCE - Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dissolvable dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.11-26-2009
20120295246BLOOD CELL SORTING METHODS AND SYSTEMS - The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.11-22-2012
20110269113METHOD OF INHIBITING BIOMATERIAL-INDUCED PROCOAGULANT ACTIVITY USING COMPLEMENT INHIBITORS - Methods for reducing or eliminating biomaterial-induced procoagulant activity in blood subjected to extracorporeal treatment that exposes the blood to the biomaterial are disclosed. The methods involve treatment of the blood, or the extracorporeal biomaterial, or both, with a complement inhibitor to inhibit C5a/C5aR-mediated tissue factor formation in the blood.11-03-2011
20080233553Method and Apparatus for Separating and Harvesting Cells From a Whole Blood Sample - An automated method and apparatus for separating and harvesting cells of interest, e.g., mononuclear cells, in a whole blood sample. The method of the invention uses the aspirating/dispensing probe of an automated sample preparation instrument to underlay a density gradient medium beneath a whole blood sample in a centrifugation tube, and the same probe is used to harvest cells of interest from a cell layer formed in the tube as a result of a centrifugation step. In harvesting cells, the probe is advanced inside the tube by a fixed, predetermined distance at which the probe tip (i.e., its aspiration port) is known to be located at, or within a predetermined distance below, the bottom of the cell layer. A predetermined volume of liquid is then aspirated through the probe tip, whereby most cells of interest (and more than 90% of those cells that can be harvested by a flawless manual method) are removed from the cell layer and collected for analysis. Preferably, the probe is caused to move laterally with respect to the tube during the aspiration of the cell layer, whereby cells offset from the center of the container are readily harvested.09-25-2008
20080280281METHOD OF ALTERING THE BINDING SPECIFICITY OF PLASMA PROTEINS BY OXIDATION-REDUCTION REACTIONS - The binding specificity of at least one plasma protein suspended or dissolved in a liquid medium is altered by exposing the protein to an oxidizing agent or an electric current sufficient to alter its binding specificity. A masked protein such as an autoantibody can be recovered from blood or blood products or extracts by oxidizing the protein to change its binding specificity.11-13-2008
20080268420Isolating Human Antibodies - The invention is drawn to a method of isolating human antibodies.10-30-2008
20080213744Method of Determining Hypoxia - A method of determining hypoxia in fetal scalp blood sampled during labor comprises the determination of total lactate dehydrogenase (LDH) in plasma obtained from the sample. The method can comprise the additional determination of K, Mg, Ca, AST, ALT, lactate in the plasma and/or blood. Increased values of one or more of LDH, Mg, Ca, AST, ALT, lactate are indicative of hypoxia in the fetus. Also disclosed is the use of a plasma separation apparatus in the method.09-04-2008
20080293035Nucleic Acid Isolation - The present invention relates to a method of isolating nucleic acid from a blood sample, said method comprising: (a) selectively isolating leucocytes from said sample by binding said leucocytes to a solid support by means of a binding partner specific for leucocytes; (b) lysing said isolated leucocytes; and (c) binding nucleic acid released from said lysed cells to said solid support. Kits for isolating nucleic acid from samples form further embodiments of the invention.11-27-2008
20080286748BLOOD COLLECTION TUBE WITH SURFACTANT - Improvements in blood collection and testing. In one aspect, an improved method of manufacturing a blood collection tube, particularly illustrated for use in sedimentation rate testing, including providing an elongated glass tube with an open first end for receiving a venipuncture blood sample of at least 1 ml, formed with a closed end opposite the first end; and applying to a substantial portion of the receptacle a containment barrier. The improvements also pertain to resulting blood collection tubes, additives for blood collection tubes that permit reliable sedimentation test data after 8 hours from the blood draw, and methods of administering health care using the aforenoted tubes, additives or both.11-20-2008
20080268421Cholesterol Sensor - A method for the determination of the amount of cholesterol in a sample is provided. The method typically provides a breakdown of the HDL and LDL cholesterol contents of the sample.10-30-2008
20090162830REAGENTS AND METHODS FOR CLASSIFYING LEUKOCYTES - A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.06-25-2009
20090130646BLOOD COLLECTION DEVICE, METHOD, AND SYSTEM FOR USING THE SAME - Disclosed is a fluid collection device wherein multiple, individual samples of fluid can be collected simultaneously. The device includes a chamber and an adapter which substantially and simultaneously distributes the blood to individual chambers with chamber specific additives. Also included is a system for using the blood collection device, preferably within a diagnostic testing laboratory.05-21-2009
20090130647METHOD AND APPARATUS FOR SORTING PARTICLES - A method and an apparatus using acridine orange for sorting particles to precisely carry out the measurement of cells in the blood are provided. The cells in the blood is sorted by the steps of staining cells in the blood with acridine orange, irradiating light on the cells in the blood, determining staining behavior using platelets as a staining indicator, and sorting the cells in the blood.05-21-2009
20090186334Non-Embryonic Totipotent Blastomere-Like Stem Cells And Methods Therefor - Human non-embryonic adult totipotent and pluripotent stem cells are isolated in a simplified serum-free and feeder cell-free process. Most remarkably, certain stem cells, and especially BLSCs, are extremely small, fail to exclude trypan blue, but are nevertheless able to proliferate from even high dilutions. Therefore, so obtained stem cells can be used to prepare true monoclonal stem cell populations, which are useful in numerous uses, including therapeutic, prophylactic, diagnostic, and research uses.07-23-2009
20090325143BLOOD SEPARATION MEDIA - Disclosed is a separation media, comprising an upstream layer, comprising fibers, wherein the upstream layer has a mean flow pore size of 8 microns or less; and a downstream layer, comprising fibers. The separation media is designed to separate red blood cells from liquid specimens such as blood and allow a filtrate, such as blood plasma, to flow from said downstream layer. Also provided is a diagnostic test device incorporating the separation media. Further disclosed is a method for separating red blood cells from a liquid specimen.12-31-2009
20090325144SWINE SPERM DILUTION MEDIA CONTAINING MILK PROTEIN - The present invention discloses the application of a protein fraction from milk in dilution media for swine sperm. Compositions that contain this protein fraction show an improved and more economical performance. The present invention relates primarily to dilution media for swine sperm aimed at maintaining and/or improving the vitality of swine sperm cells and to be applied in the artificial insemination of swine. However, it is anticipated that this invention will be useful in reproductive techniques or processes such as artificial insemination and in vitro fertilization of other species.12-31-2009
20110223579Composition for Preserving Platelets and Method of Using the Same - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state.09-15-2011
20090081632Method for Enriching Rare Cell Subpopulations From Blood - An antigen-dependent negative selection blood cell separation method is described. Rare circulating epithelial cells can be separated from blood by depleting erythrocytes from a blood sample. Erythrocytes are depleted by agglutination. The new method comprises the use of an agglutinating agent, such as an anti-glycophorin A or glycophorin B antibody, as glycophorin A or B are present on erythrocytes and not on the desired epithelial cells. With regular mixing, desired rare circulating epithelial cells do not become entrapped in the red cell agglutinate.03-26-2009
20110229870DISINTEGRATION OF CELLULAR COMPONENTS IN BODY FLUIDS - The present invention refers to a method of processing a biological fluid which comprises cellular components by a freezing/thawing treatment. The method is particularly useful for preparing biological samples for analyte detection.09-22-2011
20090202982BLOOD-VISCOSITY REDUCING AGENT - A blood-viscosity reducing agent contains a protein derived from 08-13-2009
20090220930Time Varying Electromagnetic Force Sleeve for the Expansion of Cells and Method of Using the Same - A time varying electromagnetic force sleeve wherein the time varying electromagnetic force sleeve comprises a time varying electromagnetic force source operatively connected to an electrically conductive coil that can removably receive a culture container. The present invention also relates to a method for cell expansion comprising providing a time varying electromagnetic force sleeve that is introduced to a culture container and that in use delivers a time varying electromagnetic force to cells contained within the culture container for cell expansion.09-03-2009
20090117532Pre-clinical method for monitoring serial changes in circulating breast cancer cells in mice - The CellTracks® System provides a system to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Pre-clinical studies of circulating tumor cells (CTC's) have been limited by the inability to repetitively monitor CTC's in animal models. The present invention provides a method to enumerate CTC's in blood samples obtained from living mice, using a protocol similar to an in vitro diagnostic system for quantifying CTC's in patients. Accordingly, this technology can be adapted for serial monitoring of CTC's in mouse xenograft tumor models of human breast cancer.05-07-2009
20090208919PARTICLE MATRIX FOR STORAGE OF BIOMOLECULES - Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.08-20-2009
20090208920ASSAY DEVICE AND METHOD - There is provided an assay device comprising a lid and a base, said base comprising, a sample addition zone, a reaction zone and an absorbing zone, said components being in fluid connection and being part of a fluid passage leading from the sample addition zone to the absorbing zone, wherein: (a) a sample addition well is integrated in the lid, (b) the absorbing zone consists of an area on an non-porous substrate, having substantially perpendicular projections, said projections defining a volume, which together with the volume of the fluid passage defines the sample volume subjected to the assay, and (c) at least one filter is between the sample addition well and the sample addition zone. There is further provided methods for handling samples.08-20-2009
20120141975BLOOD COMPONENT SEPARATION SYSTEM AND SEPARATION MATERIAL - The present invention relates to a separation system and a separation material for easily and rapidly separating a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid containing these blood cell components without the necessity of performing centrifugation. The separation of a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid can be accomplished by capturing white blood cells and platelets on a blood cell separation material by contacting the biological fluid with the blood cell separation material, thereby providing a red blood cell-rich fraction; and recovering a white blood cell-rich fraction by separating white blood cells captured from the resulting blood cell separation material using a separation solution.06-07-2012
20090220933DETECTION OF FETAL CELLS FROM MATERNAL BLOOD - The present application relates to methods for identification of foetal cells and generation and isolation of binding members recognising foetal cells. Said methods may further be used for other purposes relating to characterisation of biological samples and biological antigens. The methods are characterised by the applicability in situations where the interesting objects are present in a limited amount, or where the interesting objects are intermixed with other material, thus the methods are suitable for use in situations where the ratio of the interesting material compared to other material is low. The application discloses methods for use of detecting foetal cells and method of generating/isolating binding members towards antigenic material of low abundancy.09-03-2009
20090220931FUNCTIONAL IN VITRO IMMUNOASSAY - The invention relates to a method for the in vitro investigation of the effect of substances in in vivo processes and an in vitro detection method for the identification of immunomodulating compounds and/or the detection of the effect of immunomodulating compounds and the identification of apoptosis-inducing and/or necrosis-inducing compounds mediated by the immune system in in vivo processes. The methods according to the invention are particularly suitable for investigating effects of substances on cells, which are mediated by the immune system. Furthermore, the method according to the invention is suitable for in vitro monitoring of the in vivo effects before, during and/or after the administration of immunomodulating compounds and of apoptosis-inducing and/or necrosis-inducing compounds.09-03-2009
20090253115COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING THE SAME - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state.10-08-2009
20090130645METHOD FOR ASSESSING THE FIBRINOGEN CONTRIBUTION IN COAGULATION - The present invention is directed to a diagnostic method for the determination of a coagulopathy in a patient, in particular, for calculating the individual need of blood components, preferably blood platelets and/or fibrinogen and/or Factor XIII, which has to be substituted in a patient.05-21-2009
20080318203Novel Microfluidic System and Method for Capturing Cells - The invention concerns a microfluidic device and its uses for capturing subset of cells in a biological fluid. Said device comprises a chamber (12-25-2008
20100184012METHODS AND DEVICES FOR IMAGING AND MANIPULATING BIOLOGICAL SAMPLES - Methods of imaging biological samples are provided. Aspects of embodiments of the methods include freezing, thawing and imaging a biological sample, one or more times, in a manner sufficient to image the biological sample while maintaining the viability and/or structural integrity of the sample. Also provided are devices and systems for use in practicing the methods.07-22-2010
20100216111Methods and Apparatus for Reducing Protein Content in Sperm Cell Extenders - The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.08-26-2010
20100227307ERGOTHIONEINE AND/OR ITS DERIVATIVES AS A CELL PRESERVATIVE - The present invention relates to cell, tissue or derivatives thereof, preserving compositions for cells in culture, storage, or lyophilization. According to the invention ergothioneine is added as a supplement to such extender or preservative compositions. Sperm cells so treated showed increased motility when thawed compared to cells that were not treated with ergothioneine.09-09-2010
20090286221Device Comprising a Container System for a Bodily Fluid - The invention relates to a device for separating a bodily fluid, in particular blood platelet concentrate, into fractions under centrifugal force. The device comprises a flexible container system which comprises at least a first container for the fluid to be separated and a second container for a separated fraction, wherein the containers are mutually connected using a connecting conduit for a fluid, wherein a widened portion is arranged in the connecting conduit between the first and second container, wherein during use a detectable separating layer is created in the widened portion. The invention also relates to a flexible container system intended for the device, a method for separating blood platelet concentrate into fractions under centrifugal force, and a blood platelet concentrate with low leukocyte content.11-19-2009
20100221697COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING AND STORING THE SAME - The present invention relates to a method for preserving and storing platelets. The method includes the steps of admixing inactivated, functional platelets with a preservative composition comprising an effective amount of one or more pharmaceutically acceptable inhibitors of platelet activation to form preserved platelets, storing the preserved platelets at low temperature, and removing the one or more inhibitors of platelet activation from the preserved platelets by diafiltration prior to transfusion.09-02-2010
20090111087Separation method of Nucleated Cells Derived from Bone Marrow for Bone Formation - The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bone marrow; and mixing the nucleated cells with a maintenance buffer for bone formation. According to the invention, bone marrow-derived nucleated cells, which can be grafted into a site in need of bone formation or bone defect treatment, can be separated in a rapid and convenient manner. As a result, bone formation in emergency patients or patients, who require repeated surgical operations, can be effectively achieved by separating the nucleated cells for bone formation in surgical locations in a convenient and rapid manner and injecting the separated cells into the patients.04-30-2009
20080280282Method for early detection of various cancers and gastrointestinal disease and monitoring of transplanted organs - A method for the early diagnosis of breast, lung, pancreatic and colon growths and cancers as well as conditions associated with donor and recipient organ transplants, both before and after transplantation to identify and allow treatment of possible transplanted organ rejection and other disease conditions related and unrelated to the transplantation, compares the gene expression patterns from a patient's peripheral blood monocytes-lymphocyte's gene system with either the similar gene expression patterns of a normal person, or with the similar gene expression patterns of a person known to have the condition being screened for. Differences between the patient's gene expression patterns for particular genes and the normal patterns indicates the presence of the condition with the number of differences indicating the probability of the condition. Similarities between the patient's gene expression patterns for those particular genes and the patterns of a person known to have the condition indicates the presence of the condition with the number of similarities indicating the probability of the condition. Portions of the method may be performed with the use of a microfluidic machine.11-13-2008
20090035744Apparatus and Method for Separating and Storing Human Reproductive Material in a Cryotank - Apparatus and method for separating individual human reproductive material from a fertilization dish and storing the material in a cryotank for future fertilization and implantation. The apparatus includes an elongate cryostraw having a first open end and second heat-sealable open end, a channel extending between said ends, and an internal, calibrated volume VC. A displacement bulb is connected to the first end of the cryostraw for admitting and emitting the material into said cryostraw. The bulb has a total volume VB02-05-2009
20110008764HUMAN GONADAL STEM CELLS - Adult human gonadal stem cells that are capable of differentiating into cells of the mesodermal lineage and ectodermal lineage are described.01-13-2011
20100233672GRAPE POLYPHENOLICS FOR PLATELET AND BACTERIAL CONTROL - Special extracts of grape berries and Goji berries can be prepared by exposing fruit juices or preparations to an insoluble binding resin which is then extracted with soluble polyvinylpyrollidone. Grape and Goji extracts made in this way can be used to inhibit or control platelet aggregation. Grape extract has exceptional antibacterial properties and can be used to control oral bacteria and to control MRSA (Methicillin-resistant 09-16-2010
20100021878METHOD FOR CLASSIFYING AND COUNTING BACTERIA IN BODY FLUIDS - A method for distinguishing erythroblasts from bacteria by automated hematology analyzers, such as, for example, the CELL-DYN® 4000 automated hematology analyzer and the CELL-DYN® Sapphire™ automated hematology analyzer. Bacterial cells scatter light and fluoresce differently than do red blood cells, white blood cells, erythroblast nuclei, and platelets. Signals generated by bacteria are distinguishable from those of erythroblasts because the signals generated by erythroblast nuclei are sufficiently unique that erythroblast nuclei can be distinguished from signals generated by bacteria. Signals generated by platelets, lysed red blood cell ghosts, and other cell debris are blocked by the triple-trigger circuitry of the hematology analyzer, because all of the signals generated by noise are below the AND/OR thresholds. Algorithm(s) in the software of the system detect and count signals generated by bacteria by means of the location and the shape of the signals generated by bacteria and calculate the concentration of bacteria per unit of body fluid. In addition, certain body fluids, such as, for example, synovial fluid, can be pretreated with a viscosity reducing agent for a short period of time to reduce the viscosity of the body fluid prior to analyzing a sample of the body fluid by an automated hematology analyzer.01-28-2010
20100015593METHOD FOR PRODUCING A COMPOSITION FOR PROMOTING SURVIVAL OF TRANSPLANTED HEMATOPOIETIC STEM CELL - HLA matched activated lymphocytes in mononuclear cells separated from peripheral blood or umbilical cord blood are proliferated and activated. After separating and collecting, the HLA matched activated lymphocytes are employed as the main component of a composition for promoting survival of transplanted hematopoietic stem cells. The obtained composition is widely usable in, for instance, prevention of survival failure of transplanted hematopoietic stem cells and therapy for promoting the survival thereof. Although the dose of the composition varies depending on the age, conditions, etc. of a patient, a humanized antibody is administered in a dose of from 0.2 to 20 ml/kg/day to mammals including humans. The composition is administered by intravenous injection either once a day (single administration or continuous administration) or intermittently once to 3 times in a week or once in 2 or 3 weeks.01-21-2010
20090035743Assay System - The present invention provides an agglutination based assay system for determining the presence and/or amount of analyte in a sample comprising a test device having one or more capillary pathways comprising detection regions adapted for non-visual detection of a sample which is releasably engageable with a reader which comprises detection means for detecting the sample at the detection regions in each of said capillary pathways and electronic means for indicating the presence and/or amount of analyte. Also provided are the individual test device and reader components, and an assay method using the system.02-05-2009
20120244519SYSTEM AND METHOD FOR TIME-RELATED MICROSCOPY OF BIOLOGICAL ORGANISMS - The invention relates to a system and a method for determination of a value for at least one parameter describing microbial activity of individual biological organisms in a liquid sample. Images, wherein individual biological organisms may be identified, are combined to provide optical sectionings of the biological organisms and the optical sectionings are analysed to determine the value for said at least one parameter describing microbial activity of said individual biological organisms in each sample container.09-27-2012
20130157249System, Method, and Device For Preserving Blood or its Components in Gas Medium Under Pressure - A system, method, and device for preserving blood and its components is described. The system and method generally include a device having a body defining a chamber, the chamber being configured to receive at least one bag containing blood or its components, the at least one bag being permeable to gas, for example, xenon. A cover is hermetically sealable to the body. An inlet is in fluid communication with the chamber. A pressure indicator is configured to indicate pressure in the chamber, the pressure indicator including a conduit containing a liquid. A portion of the conduit is transparent such that the liquid is visible. A source of pressurized gas, such as xenon, is provided to provide the pressurized gas to the chamber.06-20-2013
20100062412METHOD OF REMOVING ABNORMAL PRION PROTEIN FROM BLOOD PRODUCTS - Provided are a method of conveniently and efficiently removing abnormal prion protein from a blood product and a method of removing leukocyte simultaneously with abnormal prion protein. The method of removing an abnormal prion protein from a blood product includes filtering the blood product through a filter filled with a carrier coated with a polymer, which is composed of three units including 20 mol % or more and 40 mol % or less of a unit originating from a hydrophobic polymerizable monomer, 5 mol % or more and 13 mol % or less of a unit originating from a polymerizable monomer containing a basic nitrogen-containing part, and a unit originating from a polymerizable monomer containing a protonic neutral hydrophilic part as the balance, and then recovering the filtered blood product.03-11-2010
20110250581SPERM CRYOPROTECTIVE MEDIA - Described herein is a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and a free radical scavenger (e.g., a reducing agent, an antioxidant).10-13-2011
20110076667Methods for Improving Fertility and Selectivity For Desired Offspring Sex in Artificial Insemination - A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.03-31-2011
20110076668BLOOD SAMPLE PROCESSING APPARATUS AND BLOOD SAMPLE PROCESSING METHOD - A blood sample processing apparatus including: a container holder securing a sample container that contains a blood sample, the sample container having a lid, the container holder coupled to a rotation driver that longitudinally rotates the sample container; and a controller that commands the rotation driver to repeatedly perform an inclining-stirring operation that includes a first process and a second process, wherein in the first process, the sample container is initially held in an upright position by the container holder and then rotated to an inclined position, and in the second process, the inclined sample container is returned to the upright position, and wherein in a final inclining-stirring operation, the second process is carried out for a longer time than previous second processes.03-31-2011
20110076669METHODS AND COMPOSITIONS FOR PRESERVING PLATELETS - The present invention relates to methods for preserving platelet. In one embodiment, the method includes the steps of mixing platelets with a platelet preservation composition to form a platelet preparation, storing the platelet preparation for a desired period of time, and removing the antiplatelet agent and the anticoagulant from the platelet preparation by diafiltration prior to transfusion of the platelets. The platelet preservation composition comprises an effective amount of an antiplatelet agent and an effective amount of an anticoagulant.03-31-2011
20110151428METHODS AND COMPOUNDS FOR INCREASING RED BLOOD CELL SURVIVAL - Methods and compositions for increasing red blood cell survival and/or inhibiting hemolysis using indole-comprising compounds are described. Such methods and compositions may be useful for blood and blood product storage and preservation applications.06-23-2011
20090023130Prevention of Transfusion Related Acute Lung Injury Using Riboflavin and Light - This invention is directed toward a method of preventing the formation of bioactive substances in a pathogen inactivated blood component. The steps include illuminating the blood component with light at a sufficient energy so that an alloxazine photosensitizer present in the blood component may be photolyzed to inactivate any pathogens which may be present; preventing the formation of bioactive substances in the pathogen inactivated blood component; and storing the pathogen inactivated blood component.01-22-2009
20120171658DEVICE AND PROCEDURE FOR THE MANUFACTURE OF BLOOD PRODUCTS - The present invention relates to a device and a procedure for the withdrawal, manufacture, storage and transportation of blood products in the widest sense, in particular a closed blood removal, processing and storages system, as well as a system suitable for the administration of medicinal products which are obtained from blood or blood products, and a corresponding procedure for the legally conformant and GMP-compliant preparation, manufacture, storage and transportation of blood products or blood components or blood constituents or blood preparations (within the meaning of the Medical Products Act AMG). The present invention in particular allows the manufacture, storage, transportation and administration of completely aliquoted autologous serum eye drops for direct application to the patient or other drugs manufactured from blood or blood products without the need for clean room laboratories.07-05-2012
20080213743Method for adjusting sedimentation rates - One or more chemicals are added to a physiological fluid to alter the density and/or shape of cells in the fluid to provide a desired sedimentation rate of the cells. In a preferred embodiment, the chemical to be added is an anticoagulant, preferably ACD.09-04-2008
20090186335METHOD OF PREPARING UNILAMELLAR VESICLES FOR THE CRYOPRESERVATION AND CULTURE OF GERM CELLS AND EMBRYOS - In order to prepare the unilamellar low-density lipid vesicles, a presolution of vegetable lipids is first prepared; a suitable quantity of lipids, between 4 and 12 mg/ml, mixed with pure water, heated and stirred for about 30 minutes; the suspension obtained is kept at 50-60° C. for one hour and stirred for 2 minutes every 20 minutes. The high molecular weight hyaluronan is added to the lipidic suspension until a final concentration of 1 mg/ml is obtained, and the suspension of lipids and hyaluronan is emulsified by sonication, pumped using a homogenising valve and immediately made to go through a polycarbonate membrane, to obtain a final suspension with 70% of the particles in suspension having a size between 5 and 15 nm, which makes it possible to use the monolayer vesicles of HA and LDL for the cryopreservation and culture of different mammalian cells, specially germ cells.07-23-2009
20110256522ARGININE-CONTAINING COMPOSITIONS AND METHODS FOR TREATING RED BLOOD CELLS - Blood storage and/or rejuvenating compositions that include D-ribose and an arginine (e.g. L-arginine, D-arginine, or a combination thereof) are disclosed herein. Such compositions can be useful in methods for treating (e.g., storing and/or rejuvenating) red blood cells.10-20-2011
20100311036Methods for Augmentation of Cell Cryopreservation - In accordance with certain embodiments of the present disclosure, a method for cryopreserving a cell is described. The method includes encapsulating a cell in a microcapsule, the microcapsule having a diameter of less than about 100 μM. The method further includes vitrifying the encapsulated cell in a vitrifying solution comprising a cryoprotectant, wherein the cell is cooled at a rate of equal to or greater than 30,000° C./min and the cryoprotectant is present at a concentration of less than or equal to 1.5 M.12-09-2010
20090239208Red Blood Cell Storage Medium For Extended Storage - Synthetic aqueous storage solutions are disclosed for use in the processing and the storing of red blood cells prepared from whole blood including cells derived from whole blood held for an extended period at room temperature.09-24-2009
20100143878METHODS AND KITS FOR ISOLATING CELLS - Disclosed are methods for differential extraction of a target component from a sample which is predominantly composed of other types of non-target cells that can be lysed using methods that do not lyse the target cells, so that the target material can be purified away from the lysed non-target material. One exemplary method is directed to isolating sperm cells from an aqueous sample and kits for performing same.06-10-2010
20100196873Systems and Methods for Cryopreservation of Cells - A fluid sample vessel includes inlet and vent tube fittings formed at one end of a container with an opposite open end closed by a needle septum. A support cap is removably engaged to the container to support the container and protect terminal ends of inlet and vent tubular branches coupled to the fittings. The support cap includes a pair of opposite legs with outwardly directed tabs for mounting within a centrifuge while supporting the cryopreservation container.08-05-2010
20090202981Triple spin, double pool and revolumization process for concentrating platelets and derivative platelet concentrate - The present invention comprises an improved method of manufacturing platelet concentrate comprising the steps of: performing a first centrifugation of anticoagulated blood, pooling a first platelet rich sample, performing a second centrifugation on the first platelet rich sample whereby the volume of the first platelet rich sample placed in each subsequent centrifuge container is greater than the extracted volume from each previously utilized container, pooling a second platelet rich sample, performing a third centrifugation on the second platelet rich sample, whereby the volume of the second platelet rich sample placed in each subsequent centrifuge container is greater than the previous extracted volume from each previously utilized container, decanting a portion of supernatant overlying a third platelet rich sample, and suspending the third platelet rich sample such as to form a highly concentrated platelet rich formulation at concentrations greater than 8 times baseline and up to 60 times baseline.08-13-2009
20110189649Laboratory Apparatus for a Controlled Environment - A controlled environment system for use in IVF, ICSI or other biological procedures has a main chamber containing one or more manipulator devices and a microscopy viewing system enabling viewing of the manipulation procedure. The proportions of gas in a multi gas chamber atmosphere set up in the main chamber can be varied and the gas atmosphere in the main chamber can be heated. A recirculation gas circuit enables recirculation of the chamber gas atmosphere, the recirculation gas circuit including a particulate filter arrangement. The chamber provides a sealed atmosphere for conducting the manipulation procedure remotely from the exterior of the chamber in an environment in which the atmosphere is sealed from the external ambient atmosphere and the chamber atmosphere is re-circulated and filtered.08-04-2011
20120308987MATRICES AND MEDIA FOR STORAGE AND STABILIZATION OF BIOMOLECULES - The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.12-06-2012
20120308986METHOD FOR PRODUCING NK CELL-ENRICHED BLOOD PRODUCT - It is intended to provide a method for producing an NK cell-enriched blood product, the method being less invasive and capable of conveniently and rapidly growing NK cells, etc. in blood collected from an organism. The NK cells in blood are stimulated with NK cell growth-stimulating factors comprising an anti-CD16 antibody, OK432, a bisphosphonate derivative or a salt thereof, or a hydrate thereof, and a cytokine. Then, the blood is cultured at a physiological cell temperature to produce an NK cell-enriched blood product.12-06-2012
20090023129WHITE BLOOD CELL DIFFERENTIATION REAGENT AND METHOD OF USE THEREOF - The present disclosure provides a white blood cell differentiation reagent comprising a fluorescent dye compound of Formula I, wherein R01-22-2009
20100304353METHODS OF IMPROVING A GENOMIC MARKER INDEX OF DAIRY ANIMALS AND PRODUCTS - The present invention provides methods for improving desirable dairy traits through the use of a genomic marker index. Also provided are methods for determining an animal's genotype with respect to multiple markers used in calculation of the genomic marker index. The invention also provides methods for selecting or allocating animals for predetermined uses, for picking potential parent animals for breeding, and for producing improved dairy products.12-02-2010
20110045455SUSPENSION MEDIUM FOR RED BLOOD CELLS - The present invention relates to a new suspension medium or diluent solution for red blood cells for use in haematological methods. The suspension medium or diluent solution for red blood cells may comprise a combination of two or more amino acids of any group, and preserves the red blood cells in the sample for at least 8 weeks.02-24-2011
20100143879APPARATUS AND METHOD FOR FILTER CLEANING BY ULTRASOUND, BACKWASHING AND FILTER MOVEMENT DURING THE FILTRATION OF BIOLOGICAL SAMPLES - The present application is directed to the separation of—a solid fraction from a fluid sample particularly a therapeutic cellular fraction from a biological sample such as a bone marrow sample by a porous filter (06-10-2010
20090191535Method of assessing metastatic carcinomas from circulating endothelial cells and disseminated tumor cells - A method for assessing cancer in test subjects is described based upon enumeration of circulating endothelial cells and/or disseminated tumor cells in a test subject. This method is used to quantify disseminated tumor cells. Correlations with circulating tumor cells provides prognostic information with high accuracy in assessing the risk of recurrence in patients with primary breast cancer.07-30-2009
20100143877Composition for preserving reproductive cells and method of using - Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian or avian reproductive sperm cells. The compositions comprise suitable reproductive cell media and antioxidant bioflavonoids. These may be comprised of Oligomeric Proanthocyanidins (OPC) which include the specific molecules of OPCs, that are namely; (epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)).06-10-2010
20090202980Cross-Beta Structure Comprising Amyloid Binding Proteins and Methods for Detection of the Cross-Beta Structure, for Modulating Cross-Beta Structures Fibril Formation and for Modulating Cross-Beta Structure-Mediated Toxicity and Method for Interfering With Blood Coagulation - The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-β structures and the biological role of these cross-β structures.08-13-2009
20120149004BLOOD COLLECTION DEVICES CONTAINING BLOOD STABILIZATION AGENT - Disclosed are devices for collecting and stabilizing blood that contain a blood stabilization agent which includes variegin or an analog thereof, a polysulfated disaccharide, or a combination thereof, each in an amount effective to stabilize blood. Methods of making and using the devices, and kits containing the devices, are also provided.06-14-2012
20090142744Method for the Analysis of a Blood Sample, and Apparatus and Reagent for Its Implementation - Method for the automatic analysis of a blood sample in which: an analysis solution containing the blood sample, a diluent, and at least one compound to lyze the erythrocytes; at least one compound to stabilize the haemoglobin in the form of a chromogenic complex, is formed in a single dilution and analysis tank, the haemoglobin level is measured in this analysis solution by spectrophotometry in the tank after the lysis of the erythrocytes; and an appropriate quantity of this analysis solution is taken from the tank on which a leucocytic differentiation is carried out by an optical elements characterized in that the analysis solution also contains at least one compound to protect the leucocytes, allowing the distinguishing of at least four main leucocyte sub-populations. A haematological analysis apparatus for the implementation of such a method is disclosed.06-04-2009
20120301869PARTICLE SEPARATION DEVICES, METHODS AND SYSTEMS - A device, system and method for the separation and collection of sperm cells into multiple subpopulations based upon sperm characteristics and for facilitating the collection of multiple subpopulations in limited space. The device can be a redirection device including one or more spaced apart tubes, where each tube has a tube inlet for collecting particles in a flow path, a tube outlet for dispensing the collected particles, and a tube body connecting the tube inlet to the tube outlet for redirecting the particles in the flow path. The device can further include a support for holding each tube in a spaced apart relationship and a securing element for securing the one or more spaced apart tubes to the support.11-29-2012
20120301868EXTENDER FOR A SEMEN SUSPENSION - The present invention belongs to the veterinary field, and particularly to the field of assisted reproduction. The invention more specifically consists of a semen extender comprising alginate and the use thereof for sperm preservation and for increasing the percentage of offspring of a certain sex.11-29-2012
20120301867RECOVERING NUCLEATED RED BLOOD CELLS AND METHOD FOR CONCENTRATING AND RECOVERING NUCLEATED RED BLOOD CELLS - Provided is a chip or the like having a particulate concentrating mechanism such as a mechanism that can selectively concentrate nucleated red blood cells contained in maternal blood and derived from a fetus and can collect the concentrated liquid rich in nucleated red blood cells, and also provided is a nucleated red blood cell concentrating/collecting method. A micro-channel chip for concentrating nucleated red blood cells has an inlet-side channel, an outlet-side channel, and a separation narrow channel provided between the inlet-side channel and the outlet-side channel. The separation narrow channel has an inner wall having a dimension through which non-nucleated red blood cells easily pass and nucleated red blood cells hardly pass, and has a means for deforming or moving part of the inner wall of the channel to have a dimension through which nucleated red blood cells easily pass. The following are also provided: a method for collecting a liquid in which nucleated red blood cells obtained by using this chip are concentrated; and a micro-channel chip for concentrating particulates other than for concentrating nucleated red blood cells.11-29-2012
20110086336Reproductive cell maintenance system - Extracts obtained from the genus 04-14-2011
20120129149REMOVAL OF OXYGEN FROM BIOLOGICAL FLUIDS - A system for reducing the concentration of oxygen in a fluid including red blood cells includes a housing, a plurality of hollow tubes extending within the housing and adapted for flow of the fluid therethrough, wherein each tube includes an inlet and an outlet, and a carrier system that reduces the concentration of oxygen at an exterior surface of the tubes to facilitate transport of oxygen from the fluid flowing through the tubes to an exterior of the tubes.05-24-2012
20100304354METHODS FOR SEPERATING FROZEN-THAWED SPERMATOZOA INTO X-CHROMOSOME BEARING AND Y-CHROMOSOME BEARING POPULATIONS - Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (12-02-2010
20090170060USE OF WHOLE BLOOD IN PCR REACTIONS - A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.07-02-2009
20090155761Method for Producing Reticulocyte Lysate and Uses Thereof - Methods for producing substantially pure porcine reticulocyte lysate are provided. In one embodiment, the methods include inducing a reduction in the hematocrit of a pig to a level of between about 20% and about 25%; extracting at least a portion of blood from the pig; isolating a fraction of the blood containing substantially red blood cells; and preparing a porcine reticulocyte lysate preparation from the isolated fraction.06-18-2009
20120064505MEASUREMENT OF MATERIAL PROPERTIES AND RELATED METHODS AND COMPOSITIONS - The invention in some aspects relates to methods, devices and compositions for evaluating material properties, such as mechanical and rheological properties of substances, particularly biological substances, such as cells, tissues, and biological fluids. In some aspects, the invention relates to methods, devices and compositions for evaluating material properties of deformable objects, such as cells. In further aspects, the invention relates to methods, devices and compositions for diagnosing and/or characterizing disease based on material properties of biological cells.03-15-2012
20100178647SIMULATION OF NORMAL FRESH BLOOD PLATELETS - The present invention provides for novel analogs of fresh human platelets for use in hematological instrument. Methods for preparing such analogs are also described.07-15-2010
20120315619METHOD AND DEVICE FOR FILTERING PLATELETS - The present invention relates to methods for removing antiplatelet agents and anticoagulants from a platelet preparation. In one embodiment, the method includes the step of flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration. In another embodiment, the method includes the step of passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.12-13-2012
20120122072METHOD AND SYSTEM FOR HEATING AND/OR THAWING BLOOD PRODUCTS - A device for heating one or more blood product units using electromagnetic (EM) energy is disclosed. The device comprises a chamber sized and shaped for containing at least one frozen blood product unit, a dielectric heating unit for applying electromagnetic (EM) energy at a plurality of frequencies to the at least one blood product unit, and a controller for operating the dielectric heating unit to heat the at least one frozen blood product unit to about a body temperature in less than 3 minutes according to a heating pattern.05-17-2012
20120122073CIS DI-AHL MODIFIED CONTROLS FOR GLYCATED HEMOGLOBIN S-A1C DERIVED FROM HEALTHY BLOOD CELLS - The invention is composition comprising red blood cells in an aqueous suspension medium and one or more cis di-ahls; wherein more than 6 percent by weight of the hemoglobin in the red blood cells is S-Alc glycated hemoglobin. In another embodiment, the invention is a method comprising contacting red blood cells in a suspension medium having a concentration of S-Alc glycated hemoglobin of greater than 6 percent by weight of the hemoglobin in the red blood cells with a sufficient amount of one or more cis di-ahls such that the concentration of S-Alc glycated hemoglobin in resulting composition as measured by high pressure liquid chromatography, immunoassay and boronate affinity methods is consistent.05-17-2012
20100248207SEPARATION METHOD OF BIOLOGICAL OBJECTS RELATIVE TO THEIR VISCOELASTIC PROPERTIES - The present invention relates to a method for the separation of biological objects in a solution which have different viscoelastic properties, wherein said method comprises a filtration step allowing the higher viscoelastic biological objects to pass through the membrane while retaining the lower viscoelastic biological objects above the membrane, and a recovery step wherein the separated lower viscoelastic biological objects are recovered above or onto the membrane and/or the separated higher viscoelastic biological objects are recovered in the filtrate. Advantageously, the biological objects are cells. More advantageously, the recovered cells are viable cells. In one preferred embodiment, the cells are tumor cells. In another preferred embodiment, the cells are fetal cells and the method finds an application in prenatal diagnosis.09-30-2010
20120231443MICROCAPSULES FOR CELL MICROENCAPSULATION - In accordance with certain embodiments of the present disclosure, a method for formation of a microcapsule is described. The method includes encapsulating a cell in a microcapsule, the microcapsule having a diameter of less than about 100 μm. The method further includes coating the microcapsule with chitosan and alginate.09-13-2012
20120129148COMPOSITIONS AND METHODS FOR THE STORAGE OF RED BLOOD CELLS - The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one nonmetabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.05-24-2012
20110183311DRY PLATELET PREPARATIONS FOR USE IN DIAGNOSTICS - The present invention provides compositions comprising freeze-dried platelets, microparticles, or both for use as a diagnostic for blood coagulation function. The invention also provides methods of diagnosing or monitoring blood coagulation function, including diagnosing or monitoring blood coagulation diseases and disorders. Kits for performing the methods of the invention are also provided.07-28-2011
20120129147METHOD OF PREPARING CONTROLS FOR GLYCATED HEMOGLOBIN S-A1C DERIVED FROM HEALTHY BLOOD CELLS - By way of summary, the present invention meets some or all of the above needs by providing in a first aspect a method comprising contacting red blood cells in a suspension medium containing mannose under conditions such that the concentration of S-Alc glycated hemoglobin is increased to greater than about 6 percent by weight of the hemoglobin in the red blood cells. Preferably the suspension medium is an aqueous suspension medium. In preferred embodiments, the method is performed at about ambient temperature, 18° C. to about 23° C.05-24-2012
20120135391Method of Blood Pooling and Storage - The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.05-31-2012
20120219940ABALONE SPERM PRESERVATION METHOD FOR THE AQUACULTURE INDUSTRY - The invention relates to a method for the preservation of sperm from abalone, in particular green abalone, 08-30-2012
20100173278MICROCAPSULES CONTAINING MAMMALS' SPERMATOZOIDS, INSEMINATION DOSE CONTAINING SAME AND METHOD FOR OBTAINING SAME - The invention particularly relates to microcapsules comprising: a core containing mammals', but not human, spermatozoids in suspension in a diluter, i.e. a liquid medium capable of preserving spermatozoids; an encapsulation matrix made of a polymeric material. The microcapsules are characterised in that said matrix is non-spermicidal and is made of at least one semi-synthetic glyceride, i.e. at least one glyceride of C8-C18 saturated fatty acids having a fusion temperature of about 35 to 47° C. The invention also relates to an insemination dose containing such microcapsules, and to a method for producing the same.07-08-2010
20120252001Method of Blood Pooling and Storage - The disclosure provides methods of making a red blood cell, plasma, and platelet products having a uniform dose and volume. The method comprises pooling a plurality of blood units, leukoreducing the blood and inactivating any pathogen contained therein. Plasma, RBCs, and platelets are then divided into uniform dose and volume units which have an extended shelf life.10-04-2012
20120251999VITRIFICATION SYSTEMS AND METHODS - The embodiments of the invention described herein relate to systems and methods for the vitrification of biological samples. Vitrification is achieved by generating nanodroplets of a solution comprising the biological sample with a means that can be automated and adapted to high-throughput applications.10-04-2012
20120077182ADDITIVE SOLUTION FOR BLOOD PRESERVATION - A method for the storage of red blood cells provides for mixing an additive solution with packed red blood cells to create a suspension of red blood cells. The method further provides for reducing the oxygen in the suspension of red blood cells; and storing the suspension of red blood cells under oxygen-depleted storage.03-29-2012
20120077181METHOD FOR THE CRYOPRESERVATION OF CELLS, ARTIFICIAL CELL CONSTRUCTS OR THREE-DIMENSIONAL COMPLEX TISSUES ASSEMBLIES - The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.03-29-2012
20100062413METHOD OF EVALUATING IBD, AMINO ACID DATA PROCESSOR, AMINO ACID DATA-PROCESSING METHOD, AMINO ACID DATA-PROCESSING SYSTEM, AMINO-ACID DATA-PROCESSING PROGRAM AND RECORDING MEDIUM - According to the method of evaluating IBD of the present invention, amino acid concentration data on the concentration value of amino acid in blood collected from a subject to be evaluated is measured, and an inflammatory bowel disease state in the subject is evaluated based on the measured amino acid concentration data of the subject.03-11-2010
20100047761CAPILLARY TRANSPORT - A method for sorting particles, in particular cells A and B. The method uses a single channel with only one input and only one output. A particle mix A and B in a fluid is introduced into the channel and particles within the channel are sorted.02-25-2010
20100273141Glycine stabilized lyophilized plasma - The invention is directed to stabilized whole-cell plasma, which retains the integrity and overall stability of the proteins and other macromolecules of the plasma. Stabilization is accomplished by the addition of glycine to plasma which allows for stabilization prior to freeze drying. Glycine, in the presence of the salt concentration in the plasma, does not recrystallize and acts as a superior stabilizer for the lyophilized plasma. The stability of the freeze dried plasma may be further improved by addition of protectants including calcium chloride, trisodium citrate, hydroxyethyl starch, ammonium sulfate and citric acid to maintain physiologic pH. Superior stability for a wide variety of plasma proteins and functions is shown. A system for the large scale preparation of sterile lyophilized plasma is also provided.10-28-2010
20100009334METHOD FOR TREATMENT AND STORAGE OF PLATELETS - Provides are improved methods for storing platelets and compositions that contain stored platelets for use in transfusions. The method entails obtaining a platelet concentrate from blood obtained from an individual and holding the platelet concentrate in at refrigerated temperatures under an atmosphere having a pressure of from 3.5 to 5 bars comprising more than 65% xenon and for at least one week. Also provided is a refrigerated composition that contains a platelet concentrate, wherein the platelet concentrate contains xenon, and wherein the platelet concentrate has been isolated from an individual for at least seven days.01-14-2010
20100009333Methods for Acoustic Particle Focusing in Biological Sample Analyzers - Methods for using acoustic focusing technology on its own or in conjunction with hydrodynamic focusing for analyzing biological samples are provided. In one application, a preferential orientation of biological particles is achieved by applying a substantially elliptical acoustic field. In another application, a sample comprising a fluid medium carrying a plurality of discrete biological particles is pre-concentrated in-line with a sample analyzer, such as a flow cytometer, where a sheath fluid is introduced after acoustic pre-concentration. In a further application, methods for acoustically separating suspended discrete biological particles of different densities from a fluid medium are discussed. The particle-free fluid medium, such as a blood-cell-free and lipid-free clear serum, may be used for chemical analysis.01-14-2010
20100291535METHOD USING MICROFLUIDIC CHIP TO SORT HIGH MOTILITY SPERM - The present invention discloses a method using a microfluidic chip to sort high motility sperm. In the present invention, sperm and a medium are respectively injected into a microchannel of a microfluidic chip via several inlets. Owing to the characteristic of microfluidics, the sperm and the medium form a sperm laminar flow and medium laminar flow in the microchannels; the sperm laminar flow and the medium laminar flow are parallel to each other. The higher motile sperm may pass through at least one laminar flow within a limited time, whereby different motility levels of sperm can be respectively collected from different outlets.11-18-2010
20100291534Methods and Systems for Isolating, Ex Vivo Expanding and Harvesting Hematopoietic Stem Cells - Disclosed herein are methods and systems for isolating, ex vivo expanding and harvesting hematopoietic stem cells. Methods and systems described herein are easy to use, time-efficient, and allow isolation, ex vivo expansion and harvest of hematopoietic stem cells either batchly or continuously.11-18-2010
20120225419METHODS AND SYSTEMS FOR PROVIDING RED BLOOD CELL PRODUCTS WITH REDUCED PLASMA - Methods and systems for processing and conditioning red blood cells are disclosed. The methods and systems may be used to make a readily transfusible red blood cell product with reduced plasma. In general, the plasma content of the supernatant of the red blood cell product is no greater than about 15%. The red blood cell products are prepared using the disclosed methods and systems remain transfusible for up to 42 days.09-06-2012
20120225418APPARATUS AND METHOD FOR SELECTING PARTICLES - The invention provides a device and a method for flow cytometric fractionation of particles contained in a fluid stream, wherein sections of the fluid stream, especially droplets if the fluid stream is a droplet stream, are irradiated with a laser. The laser disposed for the irradiation of the sections of the fluid stream can have a wavelength which is absorbed by the fluid and can have a sufficient radiation duration and radiation intensity to deflect sections of the fluid stream.09-06-2012
20120264105Composition for Preserving Reproductive Cells and Method of Using - Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian or avian reproductive sperm cells. The compositions comprise suitable reproductive cell media and antioxidant bioflavonoids. These may be comprised of Oligomeric Proanthocyanidins (OPC) which include the specific molecules of OPCs, that are, namely; (epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)).10-18-2012
20120264106Novel methods for modulating inflammatory and/or immune responses - The invention is directed to novel methods for modulating inflammatory and/or immune responses. Such methods utilize compositions comprising extraembryonic cells (herein referred to as EE cells) including but not limited to extraembryonic HLA-G positive cells (herein referred to as EHP cells) and amnion-derived multipotent progenitor cells (herein referred to as AMP cells); compositions comprising expanded EE cell populations, and/or cell lysates and/or conditioned media derived therefrom, alone or in combination with each other and/or in combination with various extracellular matrices and/or devices and/or other suitable active agents.10-18-2012
20120329036COMPOSITIONS AND METHODS FOR THE STORAGE OF RED BLOOD CELLS - The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one nonmetabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.12-27-2012
20120329035METHODS FOR IMPROVING SPERM FUNCTIONALITY - Methods for improving the functionality and/or fertility of sperm, for example, by enhancing motility and extending the lifespan of sperm in the female reproductive tract, by adding PEG to the surface of the sperm are provided. Such methods may be used in artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.12-27-2012
20130171615SELECTIVE LYSIS OF CELLS - Methods and devices for the selective lysis of cells in a sample comprising micro-organisms such as bacteria are provided. The selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.07-04-2013
20080299538Pathogen Inactivation of Whole Blood - This invention is directed toward a method of pathogen inactivating whole blood. The steps include collecting whole blood from a donor into a bag; illuminating the whole blood with light at a sufficient energy so that an alloxazine photosensitizer present in the whole blood may be photolyzed to inactivate any pathogens which may be present in the whole blood; and storing the pathogen inactivated whole blood. The invention also includes a method of separating the pathogen inactivated whole blood into components.12-04-2008
20080299537Apparatus and Methods For Selecting Capacitated Spermatozoa and Uses Thereof - The present invention relates to apparatus, systems and methods for generating a subpopulation of spermatozoa enriched for capacitated spermatozoa, by exposure of a population of spermatozoa to a suitable temperature gradient, and retrieving the enriched subpopulation of spermatozoa for further applications, such as, for diagnosis or fertility treatments.12-04-2008
20110003276BLOOD SEPARATION AND CONCENTRATION SYSTEM - This invention provides an apparatus and methods to consistently separate and concentrate selected blood components. The system includes, e.g., a computerized fluid handling system to transfer blood components between a centrifugal blood separation disc, containers and a concentrator.01-06-2011
20120322046METHOD FOR DETECTING CELL AND CELL DETECTING SYSTEM - The detection of rare cells can be achieved with high efficiency, without deteriorating the sensitivity of the detection. A detection method which enables the above-mentioned detection comprises: a first step of carrying out a pretreatment for removing erythrocytes from blood to prepare a cell suspension containing leukocytes and rare cells; a second step of providing all of the cells contained in the cell suspension produced in the first step onto an observation area having multiple holes formed thereon; a third step of taking an optical image of the cells that have been provided on the observation area; and a forth step of detecting the rare cells from the image obtained by the image taking in the third step.12-20-2012
20120270204MAGNETIC SORTING OF MAMMALIAN SPERM HAVING DAMAGED MEMBRANES - A method for magnetic sorting of mammalian sperm cells having damaged membranes is described. In an embodiment of the invention, carboxyl-group functionalized magnetic particles are conjugated to propidium iodide, the resulting composition is mixed with a sample of sperm cells, and sperm cells bound to magnetic particles are separated by magnetic-activated cell sorting.10-25-2012
20100233673Devices, systems, and methods for reducing levels of pro-inflammatory or anti-inflammatory stimulators or mediators in blood products - Devices, systems, and methods reduce levels of pro-inflammatory or anti-inflammatory stimulators or mediators in blood by selective adsorption. The devices, systems; and methods are useful in situations where abnormal levels of or unregulated or excessive interaction among pro-inflammatory or anti-inflammatory stimulators or mediators occur, or during events that do induce or have the potential for inducing abnormal production of pro-inflammatory or anti-inflammatory stimulators or mediators. The devices, systems, and methods serve to prevent, control, reduce, or alleviate the severity of the inflammatory response and disease states that are associated with abnormal levels of or unregulated or excessive interaction among pro-inflammatory or anti-inflammatory stimulators or mediators.09-16-2010
20100233671FREEZE-DRIED PLASMA FORMATS FOR THE TRAUMA CARE FIELD - Disclosed are freeze-dried plasma formats specifically designed for the trauma care field. Blood plasma is subjected to a glucose removal step, a protein fraction up-concentration step and addition of stabilizers prior to freeze-drying. Preferable stabilizers are glutamine dipeptides, glutamine and glycine. The glutamine based formulation is added direct to plasma and serves three main purposes: 1) Increases stability of plasma proteins and stabilizes pH in freeze-dried state; 2) Increases stability of plasma proteins against Gamma Irradiation and thus allows for the application of a terminal sterilization step; 3) Introduces supplements beneficial to the trauma patient.09-16-2010
20120100521Microfluidic Isolation of Tumor Cells or Other Rare Cells from Whole Blood or Other Liquids - Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood. The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may be used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria. The device may optionally be used to nondestructively capture and later to release target cells.04-26-2012
20120100524TUBULAR FLOATING ELECTRODE DIELECTRIC BARRIER DISCHARGE FOR APPLICATIONS IN STERILIZATION AND TISSUE BONDING - Disclosed is a device and method for contacting a biological substrate. A non-thermal plasma device delivers a non-thermal plasma discharge using a dielectric conduit, an igniter electrode and a RF electrode. The dielectric conduit fluidicly communicates a gas therethrough and an igniter electrode ionizes at least a portion of the gas. The RF electrode, disposed circumferentially proximate to the exterior of the dielectric conduit, generates non-thermal plasma from the ionized gas. The non-thermal plasma is discharged from the dielectric conduit and contacts a biological substrate. The non-thermal plasma discharge may be suitable for tissue bonding and sterilization applications.04-26-2012
20120100523OXYGEN DEPLETION DEVICES AND METHODS FOR REMOVING OXYGEN FROM RED BLOOD CELLS - An oxygen depletion device. The device has a cartridge; a plurality of hollow fibers extending within the cartridge from an entrance to an exit thereof; an amount of an oxygen scavenger packed within the cartridge and contiguous to and in between the plurality of hollow fibers. The hollow fibers are adapted to receiving and conveying red blood cells. There is another embodiment of an oxygen depletion device and method for removing oxygen from red blood cells.04-26-2012
20120100522STABILIZED CHEMICAL DEHYDRATION OF BIOLOGICAL MATERIAL - The present invention provides compositions and methods that enable the stabilization and storage of samples by contacting a sample with an assembly of particles, and reducing the water activity level of the contacted sample. By reducing the water activity level of the sample, the assembly of particles minimizes the degradation of the sample. Stabilizers may or may not be added to the assembly of particles to further minimize the degradation of the sample. Subsequently to storage in the assembly of particles, the samples are recoverable by eluting the assembly of particles with a fluid solution. In one embodiment, the entire assembly of particles will dissolve into the solution. In another embodiment, only part of the assembly of particles will dissolve into the solution. The assembly of particles provides the advantage that while it is porous, it comprises non-porous particulate material.04-26-2012
20100167261PLATELET STABILIZATION - Provided are methods for stabilizing mean platelet component and/or platelets in a sample comprising combining said sample with EDTA, a second anticoagulant, and one or more kinase inhibitors. Also provided are compositions for stabilizing a sample comprising platelets, and kits for storing a platelet sample.07-01-2010
20130011825METHODS AND SYSTEMS FOR REDUCING DNA FRAGMENTATION IN A PROCESSED SPERM SAMPLE - A method and system for processing reproductive cell samples and for sorting sperm with reduced levels and occurrences of DNA fragmentation compared to conventional sorting and processing methods, and for using reproductive cell and sperm cell samples with low levels of DNA fragmentation to improve viability of insemination samples fertility and success rates of assisted reproductive procedures, including artificial insemination, in vitro fertilization, intracytoplasmic injection, and other related techniques.01-10-2013
20090047654Polymer affinity matrix, a method for the production and use thereof - A polymer affinity matrix for the binding of one or more substances in a fluid or removing said substance(s) from the fluid and/or decreasing the amount or concentration thereof in said fluid with a view to preventing, eliminating or reducing undesired activation of components in said fluid is described, as well as a method for removing said substance(s) from the fluid and/or decreasing the amount or concentration thereof in said fluid, a method for the production of said matrix, use of said matrix and a kit comprising said matrix. The polymer affinity matrix comprises a solid support, a spacer and a ligand, containing arginine as a binding unit.02-19-2009
20080241815Methods for Inducing the Differentiation of Blood Monocytes into Functional Dendritic Cells - Methods are provided for inducing differentiation of blood monocytes into functional antigen presenting dendritic cells. The blood monocytes are treated by exposing the monocytes to proteins adhered to the surface of a treatment device to induce differentiation of the monocytes in to dendritic cells. The treated monocytes may be co-incubated with disease effector agents which have been rendered apoptotic or other source of antigens. The apoptotic disease effector cells are phagocytized by the maturing dendritic cells. In a preferred embodiment, the blood monocytes are treated in the plastic device without use of a photoactivatable agent and light.10-02-2008
20080233554COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING THE SAME - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state.09-25-2008
20130177900COMPOSITION FOR MAINTAINING FUNCTION OF PLATELETS - A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof:07-11-2013
20130143198BLOOD PLASMA LYOPHILIZATION PROCESS - The present invention relates to a lyophilized plasma, resulting from a mixture of plasmas originating from selected donors, which is virally attenuated and compatible with all blood groups and to the process for obtaining same.06-06-2013
20130143196METHODS AND MATERIALS FOR PROLONGING USEFUL STORAGE OF RED BLOOD CELL PREPARATIONS AND PLATELET PREPARATIONS - This document provides methods and materials for enhancing the storage capabilities of red blood cell preparations. For example, methods and materials for using CO06-06-2013
20130143195LEUKOCYTE PURIFICATION - A method for purifying leukocytes is disclosed. The method can further include recovering the purified leukocytes, or lysing the purified leukocytes.06-06-2013
20120252000REAL TIME MULTIPOINT ASSAY FOR OPTIMIZING PERFORMANCE - Described herein are methods of optimizing the performance of a mammalian semen sample with respect to outcomes such as fertility, using a multipoint assay to determine an optimum time point for preparing the semen sample for use in insemination. The multipoint assay is based upon a kinetic model of biomarker expression during sperm capacitation relative to the outcome of interest.10-04-2012
20120251998DEVICE AND METHOD FOR BLOOD TREATMENT WITH SINGLE NEEDLE OPERATION - The present invention relates to a device and a method for blood treatment in a single-needle operating mode. The blood treatment device has an extracorporeal blood circuit comprising a blood supply line leading to the inlet of a blood treatment unit and a blood return line leading away from the outlet of the blood treatment unit. Blood collecting means, for example a blood collecting container, is provided in the blood return line, said means being connected to means for storing gas, for example an air reservoir, by a flow line. The flow connection comprises two line branches, one branch being used to transfer gas from the air reservoir into the blood collecting container and the other branch being used to transfer gas from the blood collecting container into the air reservoir. The connection branch for transferring gas from the air store into the blood collecting container contains means for compressing air, for example a compressor. The device according to the present invention may be operated in such a way that the filling level of the means for collecting blood can be determined as precisely as possible.10-04-2012
20130137081METHOD OF CRYOPRESERVING SELECTED SPERM CELLS - The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.05-30-2013
20130122483KIT FOR SPERM CRYOPROTECTION - Described herein are kits for use in cryopreserving mammalian sperm including a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and monothioglycerol.05-16-2013
20130130226Microfluidics Sorter For Cell Detection And Isolation - A method of detecting one or more diseased blood cells in a blood sample includes introducing a blood sample into at least one inlet of a microfluidic device comprising one or more linear channels wherein each channel has a length and a cross-section of a height and a width defining an aspect ratio adapted to isolate diseased blood cells along at least one portion of the cross-section of the channel based on reduced deformability of diseased blood cells as compared to non-diseased blood cells, wherein diseased blood cells flow along a first portion of the channel to a first outlet and non-diseased blood cells flow along a second portion of the channel to a second outlet. The one or more channels can be adapted to isolate cells along portions of the cross-section of the channel based on cell size. In some embodiments, the one or more channels can be spiral channels.05-23-2013
20130143197Microfluidic Cell Separation in the Assay of Blood - The present invention is directed to methods for assaying blood samples to quantitate the types of white blood cells present. In addition, the invention includes equipment that can be used for these methods. One feature of the methodology is the use of micro fluidic devices for the separation of white blood cells from red blood cells.06-06-2013
20130157250METHOD FOR CRYOPRESERVATION OF HUMAN SPERMATOZOA FREE FROM SEMINAL PLASMA USING A FAST AND SIMPLE ASEPTIC VITRIFICATION-DEVITRIFICATION PROCESS; PORTABLE KIT FOR CARRYING OUT THE METHOD; AND USE OF THE SAME FOR TREATMENT OF DISORDERS RELATED TO REPRODUCTIVE FAILURES - The present invention discloses a method for cryopreservation of human spermatozoa: the method comprises providing spermatozoa free from seminal liquid; mixing the spermatozoa with vitrification medium, wherein the main cryoprotectant agent is 0.1-0.3 M sucrose; placing the sample inside straws and rapidly freezing them; refrigerating the straws with no need of liquid N06-20-2013
20110212432SEPARATION OF BLOOD CELLS FROM A BLOOD SAMPLE - Provided is a method for the separation of blood cells from a blood sample, which method comprises: 09-01-2011
20080199846Poultry semen extender - A medium for preserving and extending the viable useful life of poultry (e.g., turkey) semen, in vitro, is provided. Semen is preserved without loss of fertility for up to about 24 hours in the medium. The medium contains di-potassium phosphate, sodium glutamate, fructose, sodium acetate, mono-potassium phosphate, potassium citrate, magnesium chloride, N-tris hydroxymethyl methyl-2-aminoethane sulfonic acid, phosphatidylcholine, and zinc, and optionally sialic acid, carnitine, serine ethanolamine phosphodiesterase, or mixtures thereof. A method for extending the fertility of poultry (e.g., turkey) semen for up to about 24 hours when held in vitro at about 5° to about 10° C., involving mixing poultry (e.g., turkey) semen containing live spermatozoa with an aqueous medium containing di-potassium phosphate, sodium glutamate, fructose, sodium acetate, mono-potassium phosphate, potassium citrate, magnesium chloride, N-tris hydroxymethyl methyl-2-aminoethane sulfonic acid, phosphatidylcholine, and zinc, and optionally at least one of sialic acid, carnitine, serine ethanolamine phosphodiesterase, or mixtures thereof.08-21-2008
20080199845Compositions and Methods for Prolonging Survival of Platelets - The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.08-21-2008
20130101981PARTICLE MATRIX FOR STORAGE OF BIOMOLECULES - Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.04-25-2013
20130203042METHODS AND SYSTEMS FOR PROCESSING BIOLOGICAL FLUIDS - Methods and container systems for processing biological fluids are disclosed. The container systems include an inner container within an outer container. The inner container wall is made of a porous material of a selected porosity that allows certain components to pass through said porous wall but retains other components. A treating solution is introduced into the chamber of the outer container.08-08-2013
20110229871NUCLEOSIDE-CONTAINING COMPOSITIONS AND METHODS FOR TREATING RED BLOOD CELLS - Blood storage and/or rejuvenating compositions that include D-ribose and a nucleoside other than inosine (e.g., guanosine) are disclosed herein. Such compositions can be useful in methods for treating (e.g., storing and/or rejuvenating) red blood cells.09-22-2011
20110244443Methods, Systems and Devices for Separating Tumor Cells - Embodiments of the present disclosure are directed to the separation/capture of specific cells and/or contaminants, as well as the determination, monitoring, and treatment of cancer. Moreover, some embodiments are directed to methods, systems and devices for removing cancer, stem and/or tumor cells in vivo or in vitro from a bodily fluid to prevent or impede the proliferation of a cancer. Some embodiments provide a blood-compatible filter comprising, for example, a membrane provided with a number of openings (preferably precise) which yield minimal detrimental effect both quantitatively and qualitatively on cells present in the bodily fluid during the separation process. For example, in some embodiments, a majority percentage of circulating tumor cells are captured by a filter while a majority percentage of leukocytes, for example, are allowed to pass, where the passed leukocytes retain their vitality.10-06-2011
20130149692Methods And Apparatus For Donor Platelet Pre-Count In Centrifugal Blood Separator - A method for determining platelet pre-count in centrifugal blood processing apparatus comprising a centrifuge rotor, a separation chamber, a tubing set for conducting blood components and fluids and having an inlet line, and an outlet line. The apparatus tests flow conditions using a red light-green light sensor to determine a donor platelet pre-count.06-13-2013
20110236876Induction of and Maintenance of Nucleic Acid Damage in Pathogens Using Riboflavin and Light - A process for damaging and maintaining damage to the nucleic acids of pathogens such as white blood cells, bacteria and viruses which may be contained in blood or blood components. This process comprises adding to the blood or blood component containing pathogens an effective amount of riboflavin, and exposing the fluid to light of an appropriate wavelength to damage the nucleic acid of the pathogen and to substantially maintain the damage to the pathogenic nucleic acids to allow for subsequent transfusion into a recipient.09-29-2011
20130149691Methods, Reagents and Kits for Preservation of Nucleic Acids in Biological Samples - Provided is a nucleic acid preservative comprising at least one reducing agent, at least one chaotropic substance, at least one polyamine substance and at least one chelating agent and uses thereof, and a method for the preservation of nucleic acids in a biological sample. Further provided are kits for use in the preservation of nucleic acids in a biological sample, and more particularly, a blood sample.06-13-2013
20110275052HETEROGENEOUS INSEMINATE SYSTEM - A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.11-10-2011
20100316988METHOD AND DEVICE FOR FILTERING PLATELETS - The present invention relates to methods for removing antiplatelet agents and anticoagulants from a platelet preparation. In one embodiment, the method includes the step of flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration. In another embodiment, the method includes the step of passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.12-16-2010
20130183656COMPOSITIONS AND METHODS FOR IMPROVING THE QUALITY OF PROCESSED SPERM - The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.07-18-2013
20130183655COAGULATION CONTROLLING AGENTS AND DEVICES COMPRISING THE SAME - A device and kit and method for controlling coagulation in a blood sample. The coagulation controlling agent is at least one of citrate, a protamine salt, its homologs and derivatives, benzamidine, or para-aminobenzamidine. Additives such as water soluble polymers and sugars are also contemplated. The device and kit comprise a container that contains an effective amount of thrombin and a coagulation controlling agent. The method combines thrombin and a coagulation controlling agent to stabilize thrombin or accelerate its activity in a blood sample.07-18-2013
20110311960Method and Device for Identification of Nucleated Red Blood Cells from a Maternal Blood Sample - A method for concentrating and isolating nucleated cells, such as a maternal and fetal nucleated red blood cells (NRBC's), in a maternal whole blood sample. The invention also provides methods and apparatus for preparing to analyze and analyzing the sample for identification of fetal genetic material as part of prenatal genetic testing. The invention also pertains to methods and apparatus for discriminating fetal nucleated red blood cells from maternal nucleated red blood cells obtained from a blood sample taken from a pregnant woman.12-22-2011
20130189670Protein-Free Gamete and Embryo Handling and culture Media Products - This invention discloses a substantially protein-free cell culture solution for assisted reproductive technologies and methods of use thereof.07-25-2013
20130189669SPERM CRYOPROTECTIVE MEDIA - Described herein are methods for sperm cryoprotection and compositions comprising a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and a free radical scavenger (e.g., a reducing agent, an antioxidant).07-25-2013
20120028236COMPOSITION FOR PRESERVING PLATELETS AND METHOS OF USE THEREOF - A platelet preservation composition is disclosed. The composition comprises a photosensitizer, an inhibitor of platelet activation, and/or an anticoagulant. The preservation composition allows inactivation of pathogens in a platelet preparation while maintaining the functionality of the platelets.02-02-2012
20120094269Novel method for microbes depletion in human blood or full serum using antimicrobial photodynamic laser therapy - Treatment methods/devices are provided for attenuating/inactivating the pathogenic microbes found in biological fluids e.g. blood/blood products including human single-donor-fresh-frozen-plasma, platelet concentrate, red blood cells, blood clotting factors. An Antimicrobial Photodynamic Therapy method is used to eliminate multiple (resistant) bacteria, viral agents, fungi, parasites and other undetected or non-easily detected pathogenic microbes or particles in blood and blood products without affecting their biological properties. Resistant bacteria are difficult to be eliminated. This is especially true in the case for 04-19-2012
20130209985SAMPLE COLLECTION DEVICES WITH BLOOD STABILIZING AGENTS - Disclosed are devices for collecting and stabilizing blood or plasma and which contain an anti-coagulant, an antiplatelet agent, and a solubilization agent, and which may optionally include at least one other blood stabilization agent. Methods of making and using the devices in clinical medicine are also provided.08-15-2013

Patent applications in class MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT