Class / Patent application number | Description | Number of patent applications / Date published |
435100300 | Including freezing; composition therefor | 60 |
20080254439 | Plant Extract and Use Thereof as a Cryoprotective Agent - A cryopreservation medium comprising a protein-containing plant extract is disclosed. Methods, compositions, uses and kits for cryopreservation of biological material, such as a molecule, organelle, cell, embryo, tissue or organ, are also disclosed. | 10-16-2008 |
20080274446 | Cryopreserved human neuronal cultures - Cryopreserved cultures of post-mitotic neuronal or neural-like cells are provided having a viability after thaw of greater than 10%, typically greater than 50%. Once thawed, the cells are capable of further differentiation. In one embodiment, less than 20% of the cryopreserved cells are self-proliferating cells. The cells can be provided in a kit including a container of the cryopreserved neuronal or neural-like cells, optionally including additional cell culture reagents and materials. Method for preparing the cryopreserved neuronal or neural-like cells derived from embryonic stem cells, preferably human embryonic stem cells, are also provided. | 11-06-2008 |
20080299535 | Preservative Solution for Cells, Tissues and Organs Comprising Rare Sugar and Preservation Method with the Use of the Same - [Problem] To provide a preservative solution for cells, tissues and organs using a rare sugar and a preservation method using the solution. | 12-04-2008 |
20080299536 | Material and Methods for Nerve Grafting - The subject invention pertains to compositions and methods for promoting repair of damaged nerve tissue using nerve grafts and preparation of nerve grafts. The compositions and methods of the subject invention can be employed to restore the continuity of nerve interrupted by disease, traumatic events or surgical procedures. Compositions of the subject invention comprise one or more chondroitin sulfate proteoglycan (CSPG)-degrading enzymes that promote axonal penetration into damaged nerve tissue and nerve graft. The invention also concerns methods for promoting repair of damaged nerve tissue using the present compositions and nerve tissue treated according to such methods. The invention also includes storage solutions for nerve tissue. | 12-04-2008 |
20090004640 | Method of Paraffin-Penetration Treatment for Tissue Preparation and Apparatus for the Treatment - The present invention provides a paraffin-penetration treatment method for a tissue preparation, which is capable of enabling sufficient penetration of paraffin even into a tissue preparation, into which paraffin can hardly penetrate by a conventional paraffin-penetration treatment method. When the penetration of the paraffin into the tissue preparation is performed by immersing the tissue preparation in molten paraffin after a dehydrating and a degreasing treatments, the method comprises the steps of: immersing the tissue preparation in the molten paraffin so as to allow the paraffin to penetrate into the tissue preparation; cooling the tissue preparation so as to solidify the paraffin having penetrated thereinto; and immersing the tissue preparation in the molten paraffin again so as to melt the paraffin having penetrated thereinto, and the above described solidification treatment and melting treatment are conducted at least once for one tissue preparation. | 01-01-2009 |
20090017440 | INCREASING THE VIABILITY AND STRESS TOLERANCE OF VIABLE BIOLOGICAL MATERIAL - The present invention relates to a method for improving viability and/or stress tolerance of viable biological material and using the said material comprising applying hydrostatic pressure to said biological material; keeping the said viable biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and using the said material for any desired purpose in accordance with any useful protocol. The usage of the said biological material incorporates any techniques, protocols that are applicable in the field of assisted reproductive techniques, biotechnical and/or biotechnological manipulations. | 01-15-2009 |
20090023128 | STORAGE DEVICE FOR CRYOPRESERVATION OF BIOLOGICAL SAMPLES - A cryopreservation storage device ( | 01-22-2009 |
20090029340 | Cryoprotective Compositions and Methods of Using Same - A cryoprotective composition which comprises nanostructures, liquid and at least one cryoprotective agent is provided. | 01-29-2009 |
20090029341 | Sample Support and Sample Store, Especially For the Cryopreservation of Biological Samples - A sample support ( | 01-29-2009 |
20090123905 | Method of Preserving Early Embryos of Experimental Animals by Vitrification - This invention provides a solution for preserving mammalian early embryos or ES cells by vitrification, which comprises, as a base material, a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol as polyhydric alcohol or a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol and 25% to 35% (v/v) ethylene glycol as polyhydric alcohols and further contains 15% to 25% (v/v) Percoll® and 0.2 M to 0.5 M sucrose. This invention also provides a method for preserving mammalian early embryos or ES cells by vitrification using such solution. | 05-14-2009 |
20090170059 | Methods for Preparing Cord Matrix Stem Cells (CMSC) for Long Term Storage and for Preparing a Segment of umbilical cord for cryopreservation - Methods and kits are provided for preparation of umbilical cord fragments and cells using autologous blood or blood products, and for storage of these materials with autologous cells and blood or blood products in containers having a plurality of separable chambers. | 07-02-2009 |
20090202978 | Method and apparatus for freezing of a biological material - The present invention provides a device for freezing biological material, comprising: a cooling unit; a heat insulation unit, having an interface with said cooling unit and a common longitudinal axis passing through said interface transversely thereto; an elongated slot disposed at least in said heat insulation unit and extending along said axis, said slot comprising a first slot portion remote from said interface and a second slot portion adjacent said interface; wherein said first and second slot portions have respective first and second dimensions measured perpendicularly to said slot's axis, the former being greater than the latter. | 08-13-2009 |
20090239207 | METHOD OF ASSESSING THE VIABILITY OF THAWED CELLS - The invention relates to a method of assessing the viability of a thawed cell wherein the cell is a gamete, an embryo, a karyoplast, a putative stem cell population, a stem cell precursor population or a stem cell population. The method includes incubating the thawed cell in a culture medium including a plurality of amino acids and determining the change in concentration in the medium of at least one amino acid. | 09-24-2009 |
20090286220 | Hypothermic preservation of biological tissues and cells - A method for hypothermic preservation of biological tissue for later recovery to a viable state includes flushing the biological tissue with a gas mixture of sulfur hexafluoride or xenon and oxygen. The sulfur hexafluoride or xenon is in a concentration in the mixture between about 75 mole percent to 95 mole percent. The method includes pressurizing the biological tissue, preferably isothermically, with the mixture to a pressure that will form clathrates inside the biological tissue at a desired storage temperature in a range of about +1° C. to about +5° C. The method includes a step of cooling the biological tissue, preferably isobarically, to the desired storage temperature. Optional steps for further cooling to no colder than about −20° C. and for depressurization are provided as well as steps for recovering the hypothermically preserved biological tissue to a viable state, preferably using a recovery gas mixture. | 11-19-2009 |
20090298044 | Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens - A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation. | 12-03-2009 |
20100003662 | Appliance for cryopreserving collected tissue piece and method for cryopreserving collected tissue piece - An appliance for cryopreserving a collected tissue piece has a cutting position-specifying tool and a tissue piece cryopreserving plate for cryopreserving a tissue piece cut from an organ tissue by the collected tissue cutting position-specifying tool. The collected tissue cutting position-specifying tool has a tissue cutting portion-specifying member having a cutting portion-specifying open portion flat plate-shaped and having a predetermined width and a locking portion for locking an advance of a cutter provided on a lower surface of the tissue cutting portion-specifying member; and an open 1Q portion-pressing member which can be pressed against an upper surface of the tissue cutting portion-specifying member in such a way that the open portion-pressing member covers the cutting portion-specifying open portion. The tissue piece cryopreserving plate is a plate-shaped metal member which has a width equal to or a little larger than the predetermined width of the cutting portion-specifying open portion, is extended in a predetermined length, allows the tissue piece cut from the organ tissue by the collected tissue cutting position-specifying tool to be placed thereon, and has a lot of holes. | 01-07-2010 |
20100028851 | Multi-Lineage Progenitor Cells - Fetal blood multi-lineage progenitor cells that are capable of a wide spectrum of transdifferentiation are described. | 02-04-2010 |
20100068692 | ANTIFREEZE GLYCOPROTEIN ANALOGUES AND USES THEREOF - An antifreeze glycoprotein comprising at least one C-linked saccharide according to the following general formula: | 03-18-2010 |
20100075294 | Novel Cellular Compositions and Methods for Their Preparation - The present invention relates to novel cell (e.g., hepatocyte, etc.) compositions and methods for their preparation and use. In particular, the invention concerns methods of processing preparations of such cells so as to permit their repeated cryopreservation and thawing while retaining substantial viability. The invention also concerns preparations of cells (e.g., hepatocytes) that have been repeatedly cryopreserved and thawed. | 03-25-2010 |
20100075295 | Novel Cellular Compositions and Methods for Their Preparation - The present invention relates to novel cell (e.g., hepatocyte, etc.) compositions and methods for their preparation and use. In particular, the invention concerns methods of processing preparations of such cells so as to permit their repeated cryopreservation and thawing while retaining substantial viability. The invention also concerns preparations of cells (e.g., hepatocytes) that have been repeatedly cryopreserved and thawed. | 03-25-2010 |
20100112543 | PROCESSING SOFT TISSUE, METHODS AND COMPOSITIONS RELATED THERETO - In certain embodiments, the present invention relates to a process for preparing skin removed from a human donor, including a living human donor, and removing cellular components and forming a decellular matrix having as major components collagens and elastins while disinfecting the tissue. In other embodiments, the present invention relates to a process for treating a decellularized soft tissue by freezing the same at a plurality of decreasing temperatures at atmosphere or higher such that there is formation of ice crystals having a size greater than 2.0 and lyophilizing the soft tissue under vacuum to remove the water to less than 6% forming a porous matrix. | 05-06-2010 |
20100136512 | EMBRYO CULTURE MEDIA CONTAINING THYROID HORMONE - The disclosure relates to the use of culture media containing thyroid hormones or analogs thereof, and includes methods and uses thereof for embryo culture, embryo production, embryo maturation, improved survival of embryos and improved viability of embryos post cryopreservation. | 06-03-2010 |
20100151437 | Method for isolating cellular products by cryopreservation - Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a tissue having desired cells that are less prone to destructive freezing and undesired cells that are more prone to destructive freezing, or pre-treating a tissue to have such characteristics. The methods may involve freezing the tissue, disrupting the tissue, warming the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may thereby provide an enzyme-free or reduced-enzyme method of isolating a cellular product that is more consistent, reliable and less toxic than conventional methods. The methods may also yield an optimum quantity of cellular product that retain sufficient functional integrity to be useful as a transplantation resource. | 06-17-2010 |
20100216110 | METHOD FOR ICE-FREE CRYOPRESERVATION OF TISSUE - Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and −20° C., a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and −20° C., a rewarming step, where the tissue is warmed, and a washing step. | 08-26-2010 |
20100233670 | Frozen Viable Solid Organs and Method for Freezing Same - Disclosed is a method of freezing bulky biological material, comprising transferring heat out of said material to cool the material and increasing the rate of heat transfer during a time period when the inner portion of said biological material freezes and releases latent heat. This method allows prolonged ex-vivo preservation of solid mammalian organs, including a liver or significant portion thereof and a heart. The invention also provides a bank of deposited body organs or tissues as well as methods for operating such a bank. | 09-16-2010 |
20100248206 | Method of Isolating Stem and Progenitor Cells From Placenta - The present invention provides methods of cryopreserving stem and progenitor cells in a mammalian placenta; and methods of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta. Cells obtained by carrying out the methods can be used in a variety of therapeutic applications. | 09-30-2010 |
20100297600 | Flooding dish and method for changing media in the dish in the preparation of mammalian specimen culture and for cryo-preservation, freezing, vitrification and the thawing and warming of such specimens - A tray assembly which may include a single well or a plurality of wells that will contain specimens for cryo-preservation, freezing, thawing, or warming. The assembly will include a structure whereby a sequence of different media solutions can be introduced into a well or wells in the assembly without the need to move the specimens and without disturbing an overlying oil layer which floats on top of the media solutions. The media solutions will be introduced into the specimen wells through adjunct passages which communicate with the interior of the wells. The adjunct passages will preferably be integral with the wells and will be formed at the same time the wells are formed. Thus manufacturing the assembly of this invention will be inexpensive and repetitive. The adjunct passages can be used to infuse different media solutions into the wells at different stages of the protocols in question. The wells can be inverted cones or pyramids in shape. This shape allows the infusion of sequential media solutions beneath the overlying oil layer, without disturbing the specimen and without significantly elevating the solution level in the wells. The conical or pyramidal shapes also enable the user to readily locate and handle the specimens because the specimens will always gravitate to the nadir of the conical or pyramidal bottom of the wells. This shape also allows the user to see any media level indices placed on the side walls of the wells. | 11-25-2010 |
20110104656 | ARTIFICIAL KIDNEY PRECURSOR AND PROCESS FOR PRODUCTION THEREOF - The present invention provides an artificial kidney precursor containing a non-human mammalian metanephros separated out from a living body, wherein the metanephros has been subjected to freezing and thawing treatments outside a living body, and contains mammalian mesenchymal stem cells transferred outside a living body, and a method of production thereof. | 05-05-2011 |
20110129811 | Cryopreservation and Storage Systems and Methods - Embodiments provide improved systems and methods for preservation and storage of specimens for easy loading and releasing with rapid cooling and warming rates. Embodiments provide a system including an inner device that includes a specimen support located at or near the distal end of inner device and an outer sleeve that includes a longitudinal tube and a sample retaining region defined within the longitudinal tube adjacent the distal end. The outer sleeve can be adapted to allow the specimen support of the inner device to pass into the sample retaining region and to prevent the specimen support from contacting the distal end of the outer sleeve. A guide tube may optionally be included that facilitates loading and unloading the inner device within the outer sleeve. Optionally, a magnetic force can be applied to one or more ferromagnetic members of the inner device or the outer sleeve to maneuver the same. | 06-02-2011 |
20110177488 | BIOLOGICAL MATERIAL AND METHODS AND SOLUTIONS FOR PRESERVATION THEREOF - Provided is a preservation solution for preserving biological material at low temperature including one or more polyphenols and a method for preservation of biological material, the method includes adding the preservation solution to biological material, cooling the biological material and storing it under appropriate storing conditions. The present method may be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, and may be used with any biological material, including cells selected from RBC, WBC, MNC, UCB MNC and bacteria. In the case of RBC, also disclosed is a method for its freezing such that upon thawing, the material has less than 2% free hemoglobin. | 07-21-2011 |
20110189648 | POST-THAW SURVIVAL OF CRYOPRESERVED BIOLOGICAL MATERIAL BY HYDROSTATIC PRESSURE CHALLENGE - The present invention relates to a method for improving post-thaw survival of cryopreserved biological material comprising applying hydrostatic pressure to said biological material; keeping the said biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and freezing the said biological material using any protocol applicable thereto. | 08-04-2011 |
20120034594 | Methylocystis Strain SB2 Materials and Methods - The present disclosures provides isolated or purified compounds, each of which bind to a metal atom. Generally, the compounds are small in size (e.g, molecular weight of less than about 1 kDa) and peptidic in nature, inasmuch as the compounds comprise amino acids. In some embodiments, the compound comprises a structure of Formula I: | 02-09-2012 |
20120064504 | Media Solutions and Methods for Cryopreservation and Thawing of In Vitro Fertilization Specimens - A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the unfrozen specimens the same as the frozen specimens when recovering them from cryopreservation. | 03-15-2012 |
20120122071 | Method Of Processing Allograft Skin For Transplantation, And Cryopreserved Allograft Skin Produced Thereby - The present invention relates to a method of processing allograft skin for transplantation and a cryopreserved allograft skin produced thereby. More specifically, the present invention relates to a method in which a cryoprotectant is prepared by adding sucrose to basic constituents comprising dimethyl sulfoxide, an animal cell culture medium and fetal bovine serum, and then the resulting solution is used to subject skin tissue for transplantation to a freezing process. | 05-17-2012 |
20120190004 | METHODS AND COMPOSITIONS FOR THE CRYOPRESERVATION OF DUCKWEED - The present invention describes methods for the cryopreservation of duckweed plants and duckweed plant tissues. The methods comprise freezing a dehydrated duckweed frond colony to a cryopreservative temperature to obtain a frozen frond colony comprising at least one cryopreserved duckweed plant or a cryopreserved duckweed plant tissue. The method can comprise a dehydration step whereby a duckweed frond colony is dehydrated, and in some embodiments, can further comprise a dormancy-induction step prior to or during the dehydration step. The method further can further comprise a recovery step, wherein the frozen frond colony is thawed and a viable duckweed plant or duckweed plant tissue is recovered. Cryopreserved duckweed plants and duckweed plant tissues, and viable duckweed plants and duckweed tissues recovered therefrom are also provided. In some embodiments, the duckweed frond colony, duckweed plant, and duckweed tissue comprise a heterologous polynucleotide of interest, which can encode a heterologous polypeptide of interest. | 07-26-2012 |
20120322045 | METHOD FOR ISOLATING CELLULAR PRODUCTS BY CRYOPRESERVATION - Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a tissue having desired cells that are less prone to destructive freezing and undesired cells that are more prone to destructive freezing, or pre-treating a tissue to have such characteristics. The methods may involve freezing the tissue, disrupting the tissue, warming the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may thereby provide an enzyme-free or reduced-enzyme method of isolating a cellular product that is more consistent, reliable and less toxic than conventional methods. The methods may also yield an optimum quantity of cellular product that retain sufficient functional integrity to be useful as a transplantation resource. | 12-20-2012 |
20120329034 | Method For Producing An Acellular Dermal Matrix, And Acellular Dermal Matrix Produced By Same - The present invention relates to a method for producing an acellular dermal matrix, and to an acellular dermal matrix produced by same, and more particularly, to a method for producing an acellular dermal matrix, in which sucrose is added to base ingredients consisting of glycerol, propylene glycol, and a base solvent or solution so as to produce a cryoprotectant, the solution is injected into the skin below the epidermis and dermis from which cells have been removed, and freeze-drying is then performed. | 12-27-2012 |
20130004936 | SUPERCOOLING PROMOTING AGENT - The present invention discloses a supercooling promoting agent comprising a tannin for producing practical water which does not freeze. As the tannin, a hydrolyzable tannin such as 2,3,6-tri-O-galloyl-α,β-D-hamamelose, 1,2,6-tri-O-galloyl-β-D-glucose, and a vitrification liquid, each of which contains the supercooling promoting agent are useful as a solution or the like for storing a biological material at low temperature. | 01-03-2013 |
20130059286 | CRYOPRESERVATION OF UMBILICAL CORD TISSUE FOR CORD TISSUE-DERIVED STEM CELLS - A method of preserving an umbilical cord is disclosed. The method comprises obtaining a segment of an umbilical cord; mincing the segment of the umbilical cord into cord tissue pieces; admixing the cord tissue pieces with a cryogenic composition comprising a cryoprotectant and a protein to form a mixture; shaking the mixture for a duration of no shorter than 20 minutes and no longer than 40 minutes; and cryopreserving the mixture. | 03-07-2013 |
20130137080 | Micromanipulation and Storage Apparatus and Methods - An apparatus for micromanipulation of biological material, includes a vessel ( | 05-30-2013 |
20130252226 | TISSUE RETRIEVAL, STORAGE, AND EXPLANT CULTURE DEVICE FOR THE DERIVATION OF STEM CELLS - A device is provided for securing a tissue sample from biological material. The tissue sample is housed in bottom and top platens that are configured to promote fluid communication between the tissue sample and the exterior environment to permit transport or cryogenic fluid to contact the sample. Additionally, diskette assemblies may be provided within the platens that permit sub-samples to be separated without directly handling the tissue sample. The diskette assemblies may also be configured to promote fluid communication with the sub-sample housed therein. | 09-26-2013 |
20140011182 | METHOD AND APPARATUS FOR PREVENTION OF THERMO-MECHANICAL FRACTURING IN VITRIFIED TISSUE USING RAPID COOLING AND WARMING BY PERSUFFLATION - A method and apparatus are disclosed for avoiding fracturing, e.g., thermo-mechanical fracturing, in vitrified biological systems via rapid cooling and/or warming persufflation techniques, by reducing the domain size of fracturing and by reducing thermal gradients. Also disclosed is a system adapted to rapidly cool and warm vitrifiable vascular biological tissue by persufflation, significantly reducing cryoprotectant toxicity from that of surface cooled tissue, in which the system is constructed and configured to use one or more of helium gas, hydrogen gas, neon gas, argon gas, krypton gas, xenon gas, oxygen gas, or various gaseous compounds. The system can be operated under pressure to increase the density and heat capacity of the gas relative to its density and heat capacity at atmospheric pressure and to cool the gas by one or more of mechanical action and by the phase change of a material such as a cryogenic gas or solid. | 01-09-2014 |
20140024011 | Method for Preparing Adipose Tissue - This disclosure presents methodologies for processing, cryogenic storage, retrieval and preparation of a tissue for transplantation. As such, the methods provide a system by which tissue for transplantation can be reliably and safely processed and stored for an extended period of time until the tissue is desired to be used. | 01-23-2014 |
20140220551 | Cryopreservation of Cells and Tissue for Clinical Application - Disclosed herein are methods for cryopreserving cells and tissues under clinical conditions, allowing production of viable cell products suitable for transplantation. | 08-07-2014 |
20140342346 | METHOD OF CRYOPRESERVATION OF TISSUE DERIVED FROM PLURIPOTENT STEM CELLS - The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution. | 11-20-2014 |
20150017628 | CRYOPRESERVATION OF CELLS IN ABSENCE OF VITRIFICATION INDUCING AGENTS - The present invention relates to a method for cryopreserving biological material. In particular, the method comprises storing the biological material at a cryopreserving temperature in a composition comprising polyvinyl alcohol (PVA). wherein the composition is substantially free of vitrification-inducing agents such as DMSO and glycerol. The invention also provides methods of inhibiting ice recrystallisation and of reducing cell damage during the warming or thawing of a cryopreserved composition comprising biological material. The invention also relates to processes for producing a biological material, and related kits. | 01-15-2015 |
20150037782 | ENHANCEMENT OF ELECTROMAGNETIC FREEZING BY STABILIZATION AND OSCILLATION OF BIOGENIC MAGNETITE PARTICLES - Novel methods and systems for electromagnetic freezing are disclosed. An oscillating magnetic field can be applied in conjunction with a static magnetic field in order to align magnetic particles inside biological tissues. Such chains may be naturally present or artificially introduced. Needle-shaped electrodes may be used to produce ions and disturb the air layers insulating the tissues to be frozen. | 02-05-2015 |
20150037783 | System and Substances for Cryopreservation of Viable Cells - An improved cryopreservation process and substances can involve a cellular collection ( | 02-05-2015 |
20150313208 | USE OF CONNEXIN CHANNEL INHIBITORS TO PROTECT GRAFTS - This disclosure relates to methods to protect grafts against cell death due to preservation of the grafts prior to transplanting these grafts into patients. In particular, this disclosure discloses that connexin channel inhibitors can be used to protect against cell death associated with preservation and temporary storage of cells, tissues and organs. Preservation includes any procedure that involves a lowering of the temperature below the normal body temperature, including hypothermia (cold storage), cryopreservation and vitrification. Grafts that are protected according to this disclosure have an improved biological function. | 11-05-2015 |
20150320031 | CRYOPROTECTING AGENT, CRYOPROTECTING AND CRYOPRESERVED COMPOSITIONS, USES THEREOF, AND METHODS OF CRYOPRESERVATION - The present invention relates to a cryoprotecting agent comprising a cryprotectant being one or more of: dextrin, dextran, isomaltooligosaccharide and derivatives thereof, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation. | 11-12-2015 |
20150327538 | Method of Freezing Making Use of a Mineral Nucleator - The present invention relates to a method for freezing a water-containing quantity of a biological entity or a formulation in a vessel using a mineral nucleator, to the use of the mineral as a nucleator and to a vessel with the mineral in or on the whole or part of a surface thereof. | 11-19-2015 |
20150373968 | Small Molecule Ice Recrystallization Inhibitors And Methods Of Use Thereof - Ice recrystallization inhibitor (IRI) compounds and methods for cryopreserving umbilical cord blood are provided. The compounds are unsubstituted, mono-substituted, or di-substituted aryl-adlonamides. The methods include fractionating whole umbilical cord blood to generate a fraction comprising hematopoietic stem cells, mixing the hematopoietic stem cells with at least one IRI compound to form an IRI suspension, and freezing the IRI suspension. | 12-31-2015 |
20160000062 | PACKAGING ASSEMBLY FOR STORING TISSUE AND CELLULAR MATERIAL - The invention is an improved packaging assembly for storing, distributing, treating, mixing, and dispensing tissue and/or cellular material and/or implantable material. The packaging assembly may include pouches, tubes, and a bag made of a sealable, flexible polymeric material that is open at one end and a needle-free swabable connector attached to the pouch at the other end and acting as a port to allow for the introduction or discharge of biological solutions, rinsing solution, and/or preservation solutions into the packaging assembly. The designed thickness of the wall of the packaging assembly facilitates efficient heat/cold transfer, which is useful for successful controlled rate freezing, quick thawing, and resuscitation of viable cells or tissue. The invention is also useful for combining additional biological fluids with the cellular material or tissue, and for efficient mixing of the biological fluids with the tissue and/or cellular material in the assembly. | 01-07-2016 |
20160015024 | Method for Preparing Adipose Tissue - This disclosure presents methodologies for processing, cryogenic storage, retrieval and preparation of a tissue for transplantation. As such, the methods provide a system by which tissue for transplantation can be reliably and safely processed and stored for an extended period of time until the tissue is desired to be used. | 01-21-2016 |
20160015025 | CRYOPRESERVATIVE COMPOSTIONS AND METHODS - This disclosure describes compositions and methods related to cryoprotection of biomaterial. Generally, the cryoprotective composition includes a cryoprotective agent and magnetic nanoparticles effective for thawing a cryopreserved specimen comprising biomaterial with minimal biomaterial damage. In some embodiments, the composition is effective for thawing a cryopreserved specimen having a minimum dimension of 0.1 mm. Generally, the method includes obtaining a biomaterial cryopreserved with a cryoprotective composition as summarized above, then subjecting the cryopreserved biomaterial to electromagnetic energy of an intensity sufficient to excite the magnetic nanoparticles and thaw the biomaterial. | 01-21-2016 |
20160057992 | BIOLOGICAL SAMPLE VITRIFICATION CARRIER AND USAGE THEREOF - The present invention provides a biological sample vitrification carrier and a method for freezing a biological sample rapidly, simply, conveniently, closely and sterilely. Specifically, the carrier comprises a main body and a sealing cap. A near end of the main body and an inner bottom surface of the sealing cap are mutually sealed to form a sealed frozen sample placing region. The frozen sample placing region is provided with a frozen sample placing platform, the main body is provided with a frozen liquid circulating channel, and the sealing cap is provided with a guide hole corresponding to the frozen liquid circulating channel, so that, when the biological sample is frozen, frozen liquid circulating in the channel contacts with an outer surface of the frozen sample placing region and cools the biological sample in the frozen sample placing region. The biological sample vitrification carrier and the method in the present invention have the advantages of safety, nontoxicity and high vitrification and rewarming operation efficiency and have wide application prospects in the field of biological sample freezing. | 03-03-2016 |
20160066563 | COMPOSITIONS AND METHODS FOR COLLECTING, WASHING, CRYOPRESERVING, RECOVERING AND RETURN OF LIPOASPIRATES TO PHYSICIAN FOR AUTOLOGOUS ADIPOSE TRANSFER PROCEDURES - The invention details a cryoprotectant and the methods to prepare biological tissue and related business methods and systems. The invention is directed to methods for collecting, washing, cyropreserving, recovering, and return of lipoaspirates to physicians for autologous adipose tissue transfer procedures in patients. In an embodiment, the invention is directed to a cryoprotectant solution for cryopreserving biological tissue essentially of a polyol and a crysalloid. | 03-10-2016 |
20160249602 | METHOD FOR POOLING HEPATOCYTES | 09-01-2016 |
20180020658 | ENHANCEMENT OF CELL CRYOPRESERVATION WITH GLYCOLIPIDS | 01-25-2018 |
20190141987 | PRESERVED TISSUE PRODUCTS AND RELATED METHODS | 05-16-2019 |