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Cell analysis, classification, or counting

Subclass of:

382 - Image analysis

382100000 - APPLICATIONS

382128000 - Biomedical applications

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Class / Patent application numberDescriptionNumber of patent applications / Date published
382134000 Blood cells 69
Entries
DocumentTitleDate
20080267486METHODS FOR SELECTING CELLS WITH ENHANCED GROWTH AND PRODUCTION PROPERTIES - The disclosure relates generally to cell biology and more specifically to mechanical manipulation of cells. Methods are provided which allow robotic devices to select cell colonies that have optimum growth and bioproduction qualities resulting in a collection of cell lines that have a much higher proportion of desired growth and production characteristics. These methods greatly reduce the time and effort needed to identify cell lines with optimum combinations of viability, growth and bioproduction properties. In some embodiments, the robotic device is able to measure multiple characteristics of the colonies and use these results to select the desired colonies.10-30-2008
20080279441Cell-Image Analysis Method, Cell-Image Analysis Program, Cell-Image Analysis Apparatus, Screening Method, and Screening Apparatus - In compound screening using cell images, compounds having an effective action on cells are searched for by automated processing, to more perform existing methods for compound searching more effectively. There is provided an apparatus including an image-acquisition unit for capturing a plurality of cell images under different image-acquisition conditions; and an analysis unit for extracting a cell position and at least one feature on the basis of the plurality of cell images and for performing statistical analysis with the extracted features serving as parameters.11-13-2008
20100021038METHOD AND APPARATUS FOR CELL ANALYSIS - A method and apparatus for investigating the electroporation properties of cells suspended in a fluid. The apparatus comprises: first and second substrates spaced apart from each other; a first array of electrodes formed on a surface of the first substrate; at least one electrode formed on the surface of the second substrate facing the first array of electrodes. The at least one electrode and the first array of electrodes thereby define the upper and lower bounds of an analysis volume for receiving the fluid, the analysis volume comprising an array of volume portions. Circuitry is arranged to modify the amplitude and duration of a voltage applied to at least the first array of electrodes so as to generate and modify an electric field in one or more volume portions of the device. Thus, the 10 electroporation properties of the cells may be investigated by generating electric fields of different amplitudes or durations in one or more volume portions of the apparatus with the fluid placed therein.01-28-2010
20090196482APPARATUS, A METHOD AND SOFTWARE FOR ANALYZING A CELL IMAGE - There are provided a cell image analysis apparatus or method for judging the presence or absence of a membrane translocation reaction quickly and with good reproducibility using a fluorescence microscope. The inventive apparatus is a cell image analysis apparatus for judging the presence or absence of a membrane translocation reaction occurring in a cell owing to application of an arbitrary stimulus to the cell, characterized by comprising: a means that acquires as an image data a fluorescence microscopic image of a cell after the application of the stimulus; a means that determines a region occupied by the cell in the fluorescence image of the cell; a means that determines a contour line of the cell which has a width of a predetermined number of pixels from a periphery of the region occupied by the cell; and a means that judges the presence or absence of the membrane translocation reaction based on a brightness value on the contour line of the cell and a brightness value in an inside of the contour line of the cell.08-06-2009
20090196484Systems and Methods of Identifying Biomarkers for Subsequent Screening and Monitoring of Diseases - A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system also includes a stage onto which the grid and sample may be situated for subsequent imaging in a high contrast imager to permit identification of ultrastructural biomarkers therein. A method for generating an image of ultrastructural biomarkers from a biological sample is also provided. The generated image of ultrastructural biomarkers may be used subsequently for screening and monitoring diseases, evaluating drug and therapeutic efficacy, and assessing risks associated with a drug or therapeutic candidate, among other things.08-06-2009
20090196483Systems and Methods of Identifying Biomarkers for Subsequent Screening and Monitoring of Diseases - A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system also includes a stage onto which the grid and sample may be situated for subsequent imaging in a high contrast imager to permit identification of ultrastructural biomarkers therein. A method for generating an image of ultrastructural biomarkers from a biological sample is also provided. The generated image of ultrastructural biomarkers may be used subsequently for screening and monitoring diseases, evaluating drug and therapeutic efficacy, and assessing risks associated with a drug or therapeutic candidate, among other things.08-06-2009
20090263002Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions - The invention relates to a computer implemented method for processing of microscopic images to detect objects of interest. The method includes subjecting the microscopic image to a bandpass filtering to obtain a filtered image, wherein the bandpass filtering is such as to suppress the noise and any objects which are larger than a predetermined size; and processing the filtered image at a plurality of progressively decreasing threshold levels. The processing at each threshold level includes detecting the objects of interest using an object labelling algorithm and removing the detected objects detected at a given threshold level from the working image before proceeding to the next threshold level.10-22-2009
20110194749SYSTEM AND METHOD FOR LASER DISSECTION - Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.08-11-2011
20090190820Automated Laser Capture Microdissection - Multiple different samples are obtained from a bulk material and are separately stained. The differently stained materials look different with the different stains but also have similar characteristics. A computer is used to reorient the images so that the samples are oriented with one another. The thus oriented samples can have their like parts either reoriented. Once the stained areas are analyzed, the identified area in the unstained sample can be removed by laser capture microdissection.07-30-2009
20100074508IMAGE PROCESSING DEVICE AND MEDIUM STORING IMAGE PROCESSING PROGRAM - An image processing device can explicitly distinguish cells to be observed from cells other than those to be observed with a simple configuration. To this end, the image processing device includes a color information obtaining part obtaining at least hue from color information of each pixel of a color image, a detecting part detecting a mode value of the hue on a color space, a range setting part setting a predetermined range on the color space including the mode value of the hue detected by the detecting part as a target range, a changing part changing hue of a pixel included in the target range by virtually performing extension on the target range, and an information converting part converting color information of a pixel having hue not included in the target range into color information indicative of an achromatic color.03-25-2010
20100074507Colony Detection - The present invention provides in one aspect a method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising obtaining an image of one or more cells or cell colonies; and analyzing the image to detect a signal associated with the polypeptide of interest; wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.03-25-2010
20100074506SAMPLE IMAGING APPARATUS - A sample imaging apparatus comprising: an imaging section for imaging a stained sample including a stained cell to generate a cell image relating to the stained cell included in the stained sample; and a staining abnormality detector for detecting an abnormality relating to staining of the stained sample on the basis of the cell image generated by the imaging section, is disclosed. A sample imaging apparatus comprising: an imaging section for imaging a stained sample to generate a cell image relating to a cell included in the stained sample; and an imaging abnormality detector for detecting an abnormality relating to the imaging section on the basis of the cell image generated by the imaging section, is also disclosed.03-25-2010
20100046823SYSTEM AND METHOD FOR TRACKING THE MOVEMENT OF BIOLOGICAL MATERIALS - This invention provides an automated system and method that allows a user to track the movement of an object, such as at least one cell amongst a plurality of cells as it moves from one point to another over time in order for the user to determine how the at least one cell functions. The user is able to track the movement of the at least one cell and determine if the at least one cell structure has changed, such as the at least one cell experiencing cell division or if two or more cells merge together. This user is able to optimally track the at least one cell to find an approximate location of at least one the cell as it moves in time and distance. Thus, this invention provides the user with a means to track cell movement to study the cell function.02-25-2010
20100046824CROSS-FRAME OBJECT RECONSTRUCTION FOR IMAGE-BASED CYTOLOGY APPLICATIONS - Methods, computer software, and systems for analyzing a biological specimen (e.g., a cytological specimen) on a slide are provided. Magnified image data frames of the biological specimen are taken at different locations on the slide. An object that is not entirely contained within at least one of the image data frames is identified, and complementary portions of the object respectively located in different ones of the image data frames are matched. A fully integrated object is created from the matched object portions. Attributes of the integrated object are then analyzed.02-25-2010
20090169090TARGETED EDGE DETECTION METHOD AND APPARATUS FOR CYTOLOGICAL IMAGE PROCESSING APPLICATIONS - Edges in cytological image data are identified by obtaining a digital image of a specimen and computing a gradient image from the obtained digital image. A scaling function is applied to the grayscale image to identify regions of interest (e.g., edges of cell nuclei) in the digital image. Edges of the regions of interest are then identified based on the product of the computed gradient image and the scaling image. The scaling function may be applied to each image frame and one or more scaling thresholds are established for each frame to selectively pass, suppress, or scale pixels based on their measured intensity values. The scaled image resulting from application of the scaling function is multiplied with the gradient image to produce a targeted gradient image that identifies the edges of the region of interest. The targeted gradient image isolates edges corresponding to particular cellular structures, while rejecting other edges within the image.07-02-2009
20100080439Automatic Cell Migration and Proliferation Analysis - The disclosed methods and apparatus provide for the automatic segmentation and analysis of the overall migration rate and proliferation rate of cells that may be used with any resolution image and without the need to prepare the sample or the image before the image is analyzed. In particular embodiments, the method or apparatus of analyzing a cell image comprise performing a structure tensor analysis and/or regularization and/or a histogram-based analysis and/or a level-set analysis to classify pixels in the image into a region of interest (ROI), corresponding to cell clusters, and a non-significant region. Methods and apparatus for cell migration analysis comprise means for computing the areas of the segmented ROIs for a set of images. Methods and apparatus for cell proliferation analysis comprise means for counting the number of cells within the segmented ROIs for a set of images.04-01-2010
20120183198MICROSCOPE SLIDE COORDINATE SYSTEM REGISTRATION - Systems, methods and computer program products for mapping coordinates of various imaging stations are described. In some implementations, cells (e.g., red blood cells) in a biological specimen can be used for determining the mapping information between the imaging stations. The use of cells allows a target image (e.g., an image of a sub-region of cells in the biological specimen) taken by one imaging station to be pattern-matched to a reference image (e.g., an image showing a larger region of cells in the biological specimen that also includes the sub-region) taken by another imaging station. Once the target image is matched to the reference image, point by point correspondence (and therefore coordinates) between the target image and the reference image can be established for computing the coordinate transformation to map the imaging stations.07-19-2012
20100142794METHOD FOR UNBIASED ESTIMATION OF THE TOTAL AMOUNT OF OBJECTS BASED ON NON UNIFORM SAMPLING WITH PROBABILITY OBTAINED BY USING IMAGE ANALYSIS - An image is partitioned into sectors, and a number of sectors are selected randomly but with a probability of selection which is proportional with the likelihood of objects in the sector. For the selected sectors, the objects are measured or counted and used for estimation of the amount of objects in the entire image.06-10-2010
20100135566ANALYSIS AND CLASSIFICATION, IN PARTICULAR OF BIOLOGICAL OR BIOCHEMICAL OBJECTS, ON THE BASIS OF TIME-LAPSE IMAGES, APPLICABLE IN CYTOMETRIC TIME-LAPSE CELL ANALYSIS IN IMAGE-BASED CYTOMETRY - Among the proposals provided is a method for the analysis and classification of objects of interest, for example biological or biochemical objects, on the basis of time-lapse images, for example for use in time-lapse analysis in image-base cytometry. Images of the objects of interest, for example cells, are recorded at different moments in time and these images are subjected to a segmentation process to identify image elements as object representations or sub-object representations of objects or sub-objects of interest of objects of interest. Identified object representations or sub-object representations are then associated with one another in images of the time series and are identified as representations of the same object or sub-object or as the result of an object or sub-object. First features manifesting themselves in individual images are detected and second features manifesting themselves in a plurality of images recorded at different times are detected. The individual objects or sub-objects identified in the digital images of the series are classified on the basis of at least one classifier relating to at least one second feature, and this classification process is used as the basis for or as part of a further analysis process in relation to at least one query of interest.06-03-2010
20130044933SYSTEM AND METHODS FOR GENERATING A BRIGHTFIELD IMAGE USING FLUORESCENT IMAGES - A method for generating a brightfield type image, which resembles a brightfield staining protocol of a biological sample, using fluorescent images is provided. The steps comprise acquiring two or more fluorescent images of a fixed area on a biological sample, mapping said fluorescent image into a brightfield color space, generating a brightfield image, and optionally applying a sharpening transformation correction. Also provided is an image analysis system for generating a brightfield type image of a biological sample using fluorescent images.02-21-2013
20080273786Cell Culture Device, Image Processing Device and Cell Detecting System - In a container of a cell culture device, a light source and a camera are arranged on both sides of an incubator, and the camera or incubator can be moved in relation to each other.11-06-2008
20100092064Methods for tissue classification in cervical imagery - A rule-based unsupervised process for classifying cervical tissue by serially applying classifiers selected from the group that consists of determining size of texture region, opacity parameter, size of acetowhite regions, number of coarse and fine punctations, size of coarse and fine mosaics, size of atypical blood vessels and demographic data, so that the cervical tissue can be classified into no evidence of disease, low-grade dysplasia, high-grade dysplasia or cancer.04-15-2010
20130051651QUANTITATIVE IMAGE ANALYSIS FOR WOUND HEALING ASSAY - Illustrative embodiments of a method are disclosed, which comprise applying a texture filter to a bright field image of a wound healing assay, generating a wound mask image in response to an output of the texture filter, and determining a wound area of the wound healing assay by counting a number of pixels in the wound mask image corresponding to the wound area. Illustrative embodiments of apparatus are also disclosed.02-28-2013
20130051650SYSTEMS AND METHODS FOR TISSUE CLASSIFICATION - Methods and systems for tissue classification of a tissue sample are provided. The methods and systems transform the tissue image data to a biomarker enhanced tissue network (BETN) such that an individual cell or a sub-cellular structure in the tissue image data corresponds to a node in the BETN, define a feature vector based on a feature set representative of a tissue type of interest, cluster nodes of the BETN based on a similarity criterion of one or features of the feature vector, and classify the nodes in the tissue image data based on the grouping of the nodes of the BETN.02-28-2013
20090304257Method for Evaluating Cell Detachment, Method of Detaching Cultured Cell, Apparatus for Evaluating Cell Detachment and Automated Cell Culture Apparatus - A novel method for evaluating cell detachment which can be used in passage operation and the like in culture of adhesive cells; a method of detaching a cultured cell utilizing the same; an apparatus for evaluating cell detachment for carrying out the aforementioned method for evaluating cell detachment; and an automated cell culture apparatus in which the apparatus is used are provided.12-10-2009
20120219206High-Throughput Biomarker Segmentation Utilizing Hierarchical Normalized Cuts - A method and apparatus for obtaining segmented images of the stained regions may comprise quantifying the extent of the presence of staining of a biomarker in an original image of a sample, which may comprise selecting a domain swatch of data based upon a user specified domain knowledge; clustering the data within the original image by conducting a frequency weighted mean shift of the data within the original image to convergence, forming a hierarchical plurality of layers each having a different data resolution to form a hierarchical data pyramid; segmenting the plurality of mean shifted data images to determine in each mean shifted data image within the hierarchical data pyramid data not excluded as outside of the swatch; mapping the data not excluded as outside the swatch spatially back to the original image to create a final image; and, storing the final image on a storage medium for further analysis.08-30-2012
20120219205NONINVASIVE ASSESSMENT OF KERATINOCYTES - Embodiments herein provide methods for noninvasive assessment of keratinocytes. Digital imaging and processing of gray-levels are used to identify cells. More specifically, embodiments provide an automated algorithm that may be used to identify keratinocytes, and/or to specify the coordinates/locations of keratinocytes, through noninvasive confocal imaging.08-30-2012
20120219204PREDICTING TOXICITY OF A COMPOUND OVER A RANGE OF CONCENTRATIONS - A method of predicting hepatotoxicity of a compound. The method includes imaging cells of hepatic origin positioned within a plurality of containers to obtain imaged cellular targets, each container being treated with a different concentration of the compound, the imaging being performed using a quantitative high-content cell imaging system; quantitatively measuring the imaged cellular targets to detect changes in multiple cellular targets associated with cytotoxicity of the compound; and analyzing measurements obtained from the measured imaged cellular targets over a range of compound concentrations to determine the hepatotoxicity of the compound.08-30-2012
20130071002SYSTEM AND METHOD FOR SUPPORT OF MEDICAL DIAGNOSIS - A system and method supporting medical diagnosis made based on evaluation of images of a histopathology sample. The method includes automated generation and evaluation of a digitally-stained image (emulating the effect produced on the sample by a chosen specific dye) based at least on one of data representing an image of the HE-stained sample and empirical testing data representing samples stained with specific dyes. The system is adapted to acquire a multispectral image of the sample, to implement the digital staining of the sample's image, and to evaluate automatically the digitally-stained image contemporaneously with multispectral image acquisition. The system generates an output indicative of need to request a preparation of an actual specimen stained with chosen specific dye for further evaluation, reducing the number of samples prepared in anticipation of such request and reducing time lapsed prior to the evaluation by a histopathologist.03-21-2013
20130071004IMAGE PROCESSING APPARATUS AND IMAGE PROCESSING METHOD - An image processing apparatus for processing an image of photoreceptor cells of a fundus of an eye includes a conversion unit configured to convert the image of photoreceptor cells into an image indicating periodicity of the photoreceptor cells, and an acquisition unit configured to acquire intensity information in a plurality of directions of the image indicating the periodicity.03-21-2013
20130071003SYSTEM AND DEVICE FOR CHARACTERIZING CELLS - A diagnostic device includes a microscope configured to obtain image data on a plurality of cells and a computing device. The computing device is configured to receive the image data, identify at least a portion of each of the plurality of cells based on the received image data, determine at least one of a value of a morphological parameter for each identified at least a portion of the plurality of cells or a relative organization among the identified at least a portion of the plurality of cells, and calculate statistics for the plurality of cells based on the at least one of the determined values of the morphological parameter or the determined relative organization, the statistics including information suitable for distinguishing metastatic cells from non-metastatic cells. The diagnostic device further includes an output device configured to output the statistics for diagnosis.03-21-2013
20110038523IMAGE SEGMENTATION - A method for classifying pixels in an image, e.g. a microscopy image, as being associated with a feature of interest has been described. A color brightfield microscopy image represented by color values for an array of pixels is conventionally obtained. The image is over-segmented based on the color values to provide a plurality of groups of contiguous pixels with related color values, whereby a typical feature of interest will be represented by multiple segments. A list of pairs of segments which are adjacent to one another in the image is generated, and a difference in average color value between the segments comprising each pair is determined. Pairs of adjacent segments are then selectively joined together to form a joined segment to replace the corresponding pair of segments in the list if pre-defined joining criteria are met. The pairs are considered for joining in an order based on their respective differences in average color value. Pixels are then classified as being associated with the feature of interest based on the segments following the selective joining. Segmentation information indicating the pixels classified as being associated with the feature of interest is thus provided.02-17-2011
20110013821Image analysis method for cell observation, image-processing program, and image-processing device - An image-processing program for cell observation comprises obtaining a first image showing a plurality of cells in an observation region, the first image being captured by an imaging device, and a second image showing the observation region, the second image being captured by the imaging device a predetermined time before the first image is captured; selecting one cell as a cell of interest from the plurality of cells included in the first image; specifying cells on the periphery of the cell of interest as peripheral cells; calculating movement statistics of the peripheral cells with respect to the cell of interest on the basis of the amount of relative movement of the cell of interest and the peripheral cells in the first image and the second image; and outputting the calculated movement statistics of the peripheral cells.01-20-2011
20110013820METHODS AND SYSTEMS FOR MEASURING A PROPERTY OF A MACROMOLECULE - The present disclosure provides methods of measuring a property of a macromolecule. The methods generally involve applying an empirically learned correction term to a test metric to generate a high-accuracy measurement. The present disclosure further provides a computer program product and a computer system for carrying out a subject method.01-20-2011
20090279769METHOD FOR DETECTING RARE EVENT - Provided are methods, compositions and kits for efficiently and accurately identifying rare events in a biological sample.11-12-2009
20130064441Methods and Apparatus for Fast Identification of Relevant Features for Classification or Regression - In various embodiments, methods and apparatus are provided for automated selection of features of cells useful for classifying cell phenotype. The methods include determining a signal-to-noise ratio (S/N) for each of a plurality of pairs of features, rather than S/N for individual features. The approach is capable of quickly identifying a small set of features of imaged cells that are most relevant for classification of a desired cell phenotype from among a very large number of features. The small group of relevant features can then be used to more efficiently and more accurately classify phenotype of unidentified cells.03-14-2013
20130163844IMAGE PROCESSING APPARATUS, IMAGE PROCESSING METHOD, NON-TRANSITORY COMPUTER-READABLE MEDIUM, AND IMAGE PROCESSING SYSTEM - An image processing apparatus includes an obtaining unit, a first extraction unit, a second extraction unit, a setting unit, a filter setting unit, a generating unit, and a determination unit. The obtaining unit obtains a captured image of a test piece including a target cell having a nucleus. The first extraction unit extracts pixels which are candidates for the nucleus from pixels included in the captured image. The second extraction unit extracts a connected-pixel group which is a candidate for the target cell. The setting unit sets rectangular regions in the captured image. The filter setting unit sets a filter including regions. When the filter is disposed in each of the rectangular regions, the generating unit generates an image feature value. The determination unit determines, on the basis of whether the image feature value satisfies a condition, whether the target cell is included in the rectangular region.06-27-2013
20090232382"IN VITRO" DIAGNOSTIC METHOD FOR DISEASES AFFECTING HUMAN OR ANIMAL TISSUES - The present invention relates to an “in vitro” diagnostic method for diseases affecting human or animal tissues, in particular for the diagnosis of diseases involving inflammation and fibrosis in human or animals, more particularly for liver diseases. More particularly, the present invention relates to a method for diagnosing “in vitro” abnormal morphological conditions in human or animal tissues affected by a chronic inflammatory disease, which comprises observing an image of a biopsy sample of the human or animal body in which said abnormal condition can be detected and metrically quantifying said abnormal morphological condition, wherein said step of metrically quantifying comprises detecting the extent of the fibrotic and of the inflammatory tissue by means of: i) calculating the fractal corrected perimeter (Pf) and/or area (Af) of the collagen islets, and ii) calculating the percentage area of the clustered inflammatory cells by means of the formula ACINF/AB·100, wherein ACiNF is the actual area of the inflammatory cells belonging to clusters and AB is the area of the biopsy sample.09-17-2009
20090238436COMPOSITION FOR IMAGING REAGENT - The invention provides a composition comprising a buffer and an aminoluciferase bioluminescent imaging reagent. More specifically, the invention relates to a formulation for use with an aminoluciferase bioluminescent imaging reagent. The invention also provides a method for evaluating the efficacy of an anti-apoptotic agent over multiple time points comprising the in vivo use of a composition comprising a buffer and an aminoluciferase bioluminescent imaging reagent.09-24-2009
20090238435Multi-Exposure Imaging for Automated Fluorescent Microscope Slide Scanning - The present invention provides methods and systems that acquire digital images of a microscope slide having a large variation in pixel value brightness, like, for instance, fluorescent microscope slides. The methods and the systems generate a well contrasted composite image with preserved areas of low and high fluorescent intensity from the input images. One method includes: acquiring an image of a fluorescent microscope slide at a first exposure level resulting in a first acquired image at a first contrast level; acquiring additional images of the fluorescent microscope slide each at an exposure level different from each other and different from the first exposure, resulting in different acquired images at different contrast levels; and forming a composite image of the fluorescent microscope slide by forming a weighted sum of the intensity values of the corresponding pixels of each of the images, whereby composite image has a composite contrast level over the entire composite image that represents a weighted average of the contrast levels.09-24-2009
20090232381Method for Monitoring Cells, System for Cell-Based Assay, and Program for Cell-Based Assay - A method for monitoring cells is provided capable of determining the stage in cell cycle of a cell in a rapid and highly reliable manner.09-17-2009
20120070060METHOD OF DYNAMIC CELL TRACKING IN A SAMPLE - This invention provides a method of dynamic cell tracking in a sample comprising the steps of, generating a correspondence measure between cells in two consecutive time-elapsed images of a sample, evaluating said correspondence measure to generate events linking individual cells in the two images, the events being selected from the group: unchanged, cell removal, cell migration, cell collision and cell division, and providing a tracking output of events linking individual cells in the two images. In the invention, the step of establishing linking events involves verifying the correctness of generated cell collision and cell division events, by calculating an adaptive threshold value for each such event and comparing the threshold value with an event parameter. There is further provided a system for dynamic cell tracking in a sample.03-22-2012
20120114219IMAGE PROCESSING APPARATUS, INCUBATION OBSERVING APPARATUS, AND IMAGE PROCESSING METHOD - An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.05-10-2012
20120114218HIGH FIDELITY COLOUR IMAGING OF MICROBIAL COLONIES - A system and an associated method of imaging microbial colonies in high fidelity and in colour. Differently coloured light sources are turned on and off in sequence and for each illumination colour an image is captured by a monochrome camera. The resultant respective monochrome images are combined into a composite colour image of the microbial colonies.05-10-2012
20120114216Microscopy Analysis Technique - A method and apparatus for the analysis of cell nuclei may use a system where images captured from a bright field microscope are analysed and information is retrieved that give quantitative information of the different type of chromatin within the cell nuclei for example Hetrochromatin and Euchromatin. The method is based on segmented cell nuclei. Quantitative information is determined related to the greyscale distribution of the different areas of the chromatin types and the object structure within these different areas.05-10-2012
20110019898CELL-IMAGE ANALYZING APPARATUS - A cell-image analyzing apparatus is provided with a computer, for classifying cells using plural channels of fluorescence cell images on a specimen that contains plural kinds of cells and is stained with specific fluorochromes in accordance with the kinds of cells. The cell image analyzing apparatus has an image analysis software that makes the computer function as a region delimiting mean for delimiting cell nucleus regions and cytoplasm regions in each of the plural channels of fluorescence cell images; a morphologic characteristic detecting means for detecting a morphologic characteristic on the cell nucleus regions or the cytoplasm regions delimited via the region delimiting means; and a cell classifying means for classifying the cells into kinds in accordance with the morphologic characteristic on the cell nucleus regions or the cytoplasm regions detected via the morphologic characteristic detecting means.01-27-2011
20120082363Method for Displaying a Vessel of a Particular Biological Subject - A method for displaying a vessel of a particular biological subject is proposed. The particular object is inserted into the vessel. The deformation of the vessel is predicted by an inserted object on the basis of experience. This experience is quantified by assigning values to particular parameters, the parameters providing information concerning characteristics of the patient, characteristics of his/her vessel and information about the interventional instrument inserted.04-05-2012
20120263368MEDICAL IMAGE PROCESSING APPARATUS - According to one embodiment, storage unit stores three-dimensional function image data concerning a function index of the heart. The extraction unit extracts a myocardial region from the three-dimensional function image data. The normalization unit normalizes the distance between the inner wall and outer wall of the myocardial region with a predetermined numerical value range. The generation unit generates a bull's eye map expressing a spatial distribution of pixel values at positions on the myocardial region by two-dimensional polar coordinates. The positions correspond to predetermined values in the predetermined numerical value range. The display unit displays the bull's eye map.10-18-2012
20130163845System For Detecting Infectious Agents Using Computer-Controlled Automated Image Analysis - A method for providing quantitative information regarding the extent of infection of host cells by an infectious agent. A microscope image of a specimen of a bodily fluid is analyzed using image processing techniques to quantify the percentage of the area of the specimen that is infected.06-27-2013
20110280467METHOD FOR IMAGING AND DIFFERENTIAL ANALYSIS OF CELLS - Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.11-17-2011
20110280466SYSTEMS AND METHODS FOR GENETIC IMAGING - Sequence data, e.g., genetic sequence data such as nucleic acid or amino acid sequences, can be represented in Genetic Images, as defined herein, that provide a compact, portable image that can be analyzed electronically (e.g., by computer) or optically, e.g., visually or by optical scanning devices. New methods and systems are described by which sequence data is first converted into a numeric data set, which is, in turn, encoded to form a Genetic Image. The Genetic Image can be traced backwards to determine the original sequence data.11-17-2011
20100266192COUNTING BIOLOGICAL AGENTS ON BIOLOGICAL GROWTH PLATES - The invention is directed to counting techniques for counting biological agents on a biological growth plate or similar medium. In order to automate the counting of biological agents, a biological growth plate is inserted into a biological scanning unit. Upon insertion of the biological growth plate, the biological scanning unit generates an image of the plate. Then, the amount of biological agents that appear in the image, such as a number of bacteria colonies, can be counted or otherwise determined using image processing and analysis routines performed either by the scanning unit or an external computing device, such as a desktop computer, workstation or the like. A variety of counting rules are described herein that can be used to improve the accuracy of automated counts of biological agents on a biological growth plate.10-21-2010
20110129138SYSTEM FOR DETECTING INFECTIOUS AGENTS USING COMPUTER-CONTROLLED AUTOMATED IMAGE ANALYSIS - A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.06-02-2011
20110299756METHOD AND DEVICE FOR SEGMENTING A DIGITAL CELL IMAGE - A method and a device for segmenting a digital image of biological cells, a method and a device for analyzing the dynamic behavior of biological cells and a method and a device for visualizing the dynamic behavior of biological cells. To provide a segmentation method that has minimal technical requirements, does not necessitate any special preparation or manipulation of the cells to be observed and which requires as few assumptions as possible to be made about the properties of the cells, it is proposed that the method comprises the following steps: determining a maximum gradient for each pixel of the image as the maximum difference between a pixel value of the pixel and the respective pixel values of all or selected neighboring pixels, determining a segmentation threshold value using the frequencies of the maximum gradients, classifying the images into an object class and an environment class using the segmentation threshold value and forming a segmentation zone of the digital image using a class merging method, in particular a region growing method.12-08-2011
20110286654Segmentation of Biological Image Data - Described herein are systems and methods for automated segmentation of image data. According to one aspect of the present technology, systems and methods are provided for detecting regions of interest within biological images. In particular, first and second images of first and second biological samples are received, wherein one or more routine stains have previously been applied to the first biological sample. A region of interest in the first image may be segmented to generate a boundary. The boundary may then be transferred to the second image to segment a corresponding region of interest in the second image.11-24-2011
20090202131AUTOMATED MICROSCOPIC SPERM IDENTIFICATION - A system for automatically identifying sperm cells in a smear on a microscope slide, including capturing a first digital color image within an area of interest on the microscope slide. The first digital color image is split into a plurality of component color space images and stored into a plurality of memory spaces. The component color space images are manipulated by mathematical functions, logical functions or a combination of mathematical and logical functions to produce a result image. Thresholding the result image creates a binary result image which is processed with particle (blob) analysis. A set of blob factors is applied to identify probable sperm cells; and a list of universal coordinate system positions of the probable sperm cells is created.08-13-2009
20090202130Image based quantitation of molecular translocation - The use of an imaging system, cell compartment markers, and molecular markers in methods for correlating the movement of molecules within a cell to a particular compartment are provided, including measuring and correlating molecule movement in adherent and non-adherent cells.08-13-2009
20110293165Epithelial Structure Detector and Related Methods - A method for training a classifier to be operative as an epithelial texture classifier, includes obtaining a plurality of training micrograph areas of biopsy tissue and for each of the training micrograph areas, identifying probable locations of nuclei that form epithelia, generating a skeleton graph from the probable locations of the nuclei that form the epithelia, manually drawing walls on the skeleton graph outside of the epithelia to divide the epithelia from one another, and manually selecting points that lie entirely inside the epithelia to generate open and/or closed geodesic paths in the skeleton graph between pairs of the selected points. Data is obtained from points selected from the walls and the paths and applied to a classifier to train the classifier as the epithelial texture classifier. A method and detector for detecting epithelial structures includes applying a sample micrograph area of biopsy tissue to an epithelial texture classifier; identifying probable locations of nuclei that form epithelia of the sample micrograph area with the epithelial texture classifier, generating a skeleton graph from the probable locations of the nuclei that form the epithelia of the sample micrograph area, determining a set of open and/or closed geodesic paths in the skeleton graph of the sample micrograph area; and determining a set of the epithelial masks using the open and/or closed epithelial paths of the sample micrograph area.12-01-2011
20100040276METHOD AND APPARATUS FOR DETERMINING A CELL CONTOUR OF A CELL - A method for determining a cell contour of a cell having a cell nucleus and a cytoplasm in an image of the cell includes determining nucleus candidate pixels belonging to the nucleus. Further, the method comprises determining a pixel within the area formed by the nucleus candidate pixels for obtaining a central nucleus candidate pixel, determining a first edge candidate pixel as a pixel on a predetermined path leading away from the central nucleus candidate pixel by determining a change from a first section to a second section of a color space, and finding edge candidate pixels leading away from the first edge candidate pixel forming a boundary surrounding the cell, via a path-finding algorithm tending to prefer smaller path lengths and paths through pixels in the second section of the color space.02-18-2010
20120269418ANALYZING THE EXPRESSION OF BIOMARKERS IN CELLS WITH CLUSTERS - A data set of cell profile data is stored. The cell profile data includes multiplexed biometric image data describing the expression of a plurality of biomarkers with respect to a field of view in which individual cells are delineated and segmenting into compartments. Each cell in the field is assigned to a single cluster in a selected set of clusters based on cell similarity; cell similarity is determined based on a plurality of selected cell attributes. The proportion of cells in each cluster is observed. The observed proportions are examined for an association with a diagnosis, a prognosis, or a response derived from a known association of the selected set of clusters with at least one piece of meta-information including a field of view level assessment or a patient-level assessment.10-25-2012
20120269417COMPUTER-AIDED STAINING OF MULTISPECTRAL IMAGES - A system and method for digital staining of an image of biological tissue acquired with the use of multispectral imaging system. The method includes spectral enhancement of the originally-acquired image, which results in differentiation of colorimetrically-similar components of the tissue, and a linear mapping of the spectrally-enhanced image to an estimated target image that represents the spectral response of the tissue manually stained with a target stain.10-25-2012
20120269419ANALYZING THE EXPRESSION OF BIOMARKERS IN CELLS WITH MOMENTS - A stored data set comprises cell profile data including multiplexed biometric image data describing the expression of biomarkers. The data set includes an association of the cell profile data with a field of view level or a patient-level assessment. Cell features are calculated based on the cell's expression of each of the biomarkers. Moments of cell features are calculated from the data set, and combinations are examined for an association with an assessment. A predictive set of moments is selected based on the performance of the combination.10-25-2012
20090087075Observation device - An observation device that observes a test specimen such as cultured cells or the like includes: an illumination device that illuminates the test specimen; an image-capturing device that acquires an image of the test specimen illuminated by the illumination device; a storage unit that stores correlation data manifesting a correlation relationship between an occupation ratio of the test specimen within a culture vessel, and statistically processed data regarding to luminance information of the test specimen; and a calculation unit that obtains the statistically processed data based upon luminance information in a specified color of the test specimen captured by the image-capturing device, and calculates the occupation ratio of the test specimen within the culture vessel using the correlation data in the storage unit.04-02-2009
20090190821PATHOLOGICAL DIAGNOSIS SUPPORT - A pathological diagnosis support apparatus into which a digital color image showing a stained tissue sample is input, the apparatus including: a display that performs a display operation; and an image processor that when the digital color image is input, extracts cell nucleus areas, cytoplasm areas and glandular cavity areas, respectively, based on luminance values of pixels of the digital color image, measures basic feature quantities representing features of shapes of the respective cell nucleus areas, cytoplasm areas and glandular cavity areas, which have been extracted, determines whether or not a particular kind of area that appears in a limited case according to the disease state of the tissue sample exists, for each of the cell nucleus areas, the cytoplasm areas and the glandular cavity areas based on the luminance values and the basic feature quantities, and measures structure feature quantities representing ways in which the cell nucleus areas are positioned, based on the basic feature quantities of the cell nucleus areas, and if the particular kind of area exists, extracts the particular kind of area and makes the display show it together with the structure feature quantities and the cell nucleus areas.07-30-2009
20100061617User interface method and system for management and control of automated image processing in high content screening or high throughput screening - A user interface method and system for controlling automated image processing operations of HCS and/or HTS systems includes a graphical interface to enable user designation of an image naming convention, image sources and destinations, image processing channels, processing parameter values, and processing spatial designations.03-11-2010
20090141960IMAGE PROCESSING APPARATUS AND COMPUTER PROGRAM PRODUCT - An image processing apparatus includes a recognition unit that recognizes each cell area corresponding to a living cell from a series of observation images captured at observation time points; a measurement unit that measures cell characteristic amounts of each cell; a tracking unit that determines whether a cell area captured at a processing target time point and a cell area captured earlier than the processing target time point has an identity with each other, and associates the cell areas which have an identity with each other; and a cell division detection processing unit that measures relative position information between a target cell area to be processed and a peripheral cell area positioned around the target cell area, determines whether cell division has occurred in a living cell based on at least the relative position information, and detects a target cell area on which cell division has occurred.06-04-2009
20100266191METHOD FOR QUANTIFYING AN UNDERLYING PROPERTY OF A MULTITUDE OF SAMPLES - An approach for quantification of an underlying property of a multitude of samples, e.g. cellular biological samples, is described. In an exemplary application, input measures are mean pixel intensities from different segments of individual cells on original and filtered images. In another exemplary application, input measures are pixel intensities from different cells on a series of time-gated images. The set of input measures are expected to have a linear relationship to the underlying property. Latent variables are (1) a scalar noise factor responsible for intensity fluctuations in all segments of a cell at a time and (2) the main underlying property of the process responsible for observed changes on images. Linear coefficients for latent variables are determined using noise minimization while constraining the mean signal from positive and negative control samples to the corresponding theoretical values. The method is well suited for high throughput applications like drug screening.10-21-2010
20100260407Viewing Digital Slides - Virtual slide image data and corresponding information are stored in a data storage area on a virtual slide image server. A client viewer requests image data at a particular resolution. The image server obtains corresponding image data from the data storage area at a resolution nearest to the requested resolution. The image data is then sent to the client viewer. The client viewer receives the image data and scales the image data to the requested resolution prior to displaying the image data.10-14-2010
20090285469VISUAL-SERVOING OPTICAL MICROSCOPY - The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time; quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.11-19-2009
20100098317CELL FEATURE AMOUNT CALCULATING APPARATUS AND CELL FEATURE AMOUNT CALCULATING METHOD - A cell feature amount calculating apparatus is provided that is capable of capturing the state of nuclear DNA. This cell feature amount calculating apparatus includes an image input unit that inputs an image of a cell, a cell nucleus region extracting unit that extracts a cell nucleus region of the cell from the image, a standard contour length calculating unit that calculates a standard contour length of the cell nucleus region, a contour length sequence calculating unit that extracts, for each threshold value of a plurality of different threshold values, a specific region which is a region having a pixel value larger than or equal to the threshold value from the cell nucleus region and calculating a contour length sequence by calculating a contour length of the specific region, and a contour complexity calculating unit that calculates a feature amount of the cell based on the standard contour length and the contour length sequence.04-22-2010
20120106822METHOD FOR DETERMINING THE STATE OF A CELL AGGREGATION, IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING THE METHOD, AND METHOD FOR PRODUCING A CELL AGGREGATION - An image processing program obtains a first image and a second image taken by an imaging device at a predetermined time interval; performs block matching, using the luminance distribution of a local region of the first image as a standard, in a vicinity including the corresponding position of the second image, taking the degree of approximation of the region with the highest degree of matching as a representative degree of approximation, sequentially moving the local region, and calculating the representative degree of approximation for individual parts of the cell aggregation; and outputting multi-layering information corresponding to the calculated representative degree of approximation. The image processing program outputs multi-layering information by which the state of change of the cell aggregation toward becoming multi-layered can be decided from the first and second images.05-03-2012
20120106821IMAGE-BASED RISK SCORE-A PROGNOSTIC PREDICTOR OF SURVIVAL AND OUTCOME FROM DIGITAL HISTOPATHOLOGY - The present invention relates to an image-based computer-aided prognosis (CAP) system and method that seeks to replicate the prognostic power of molecular assays in histopathology and pathological processes, including but not limited to cancer. Using only a tissue slide samples, a mechanism for digital slide scanning, and a computer, the present invention relates to an image-based CAP system and method which aims to overcome many of the drawbacks associated with prognostic molecular assays (e.g. Oncotype DX) including the high cost associated with the assay, limited laboratory facilities with specialized equipment, and length of time between biopsy and prognostic prediction.05-03-2012
20090263003Method for investigating digital images for cancer based on parameters using samples in images - A method for dividing value space of parameters into ranges using samples wherein these ranges are used for detection of cancer in a region or segment in a digital image. A method for assisting the investigation in detection of cancer in a region in a first digital image of a human body or a human body part using ranges of parameters computed using samples. A method for performing medical diagnostics for detection of cancer over a computer network.10-22-2009
20090274360Predetermined Site Luminescence Measuring Method, Predetermined Site Luminescence Measuring Apparatus, Expression Amount Measuring Method, and Measuring Apparatus - An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples. An predetermined site luminescence measuring apparatus 11-05-2009
20090141959METHODS AND SYSTEMS FOR REMOVING AUTOFLUORESCENCE FROM IMAGES - Method and systems for removing any inherent autofluorescence associated with a biological material, comprising: acquiring a first reference image of the biological material; acquiring a first set of one or more images of the biological material using one or more filters corresponding to one or more information dyes; applying the one or more additional dyes to the biological material and then acquiring a second set of one or more images comprising a separate image of the biological material with each of the filters corresponding to the information dyes and a second reference image of the biological material; coregistering the first and second reference images; and then removing any inherent autofluorescence exhibited in the informational images acquired.06-04-2009
20090279770METHOD AND APPARATUS FOR DETECTION OF RARE CELLS - Fluorescent dye-tagged rare cells are disposed in a biological fluid layer contained in an annular gap (11-12-2009
20090290780ANALYSIS METHOD FOR CHEMICAL AND/OR BIOLOGICAL SAMPLES - An analysis method for chemical and/or biological samples, particularly chemical and/or biological samples comprising cells, includes the following steps: 11-26-2009
20080212866Quantitative, multispectral image analysis of tissue specimens stained with quantum dots - A biological sample such as a tissue section is stained with one or more quantum dots and possibly other fluorophores (total number of fluorophores N). A camera coupled to a microscope generates an image of the specimen at a plurality of different wavelengths within the emission spectral band of the N fluorophores. An analysis module calculates coefficients C09-04-2008
20080212867Use of Endogenous Fluorescence to Identify Invading Metastatic Breast Tumor Cells - The present invention broadly provides methods and systems for detecting, identifying, and characterizing conditions, including diseases and other disorders in human or other animal subjects, by analyzing fluorescence from endogenous flavin adenine dinucleotide (FAD) fluorophors present in biological materials and samples. In particular embodiments, the invention relates to conditions of the human breast including cancers such as carcinoma. Methods and systems are provided for detecting, locating, and characterizing tumors and precancerous tissue via nonlinear optical imaging techniques capable of accurately characterizing fluorescence intensities and fluorescent lifetime parameters from endogenous FAD fluorophors present in a test tissue sample.09-04-2008
20110206262IMAGE-BASED METHODS FOR MEASURING GLOBAL NUCLEAR PATTERNS AS EPIGENETIC MARKERS OF CELL DIFFERENTIATION - The invention provides methods for determining the differentiation state of cells. The methods include non-invasive, non-perturbing, automatable, and quantitative methods of analysis of cell colonies, individual cells, and/or cellular structures.08-25-2011
20100128961SYSTEM AND METHOD FOR THE AUTOMATED ANALYSIS OF CELLULAR ASSAYS AND TISSUES - A method for providing an image of a biological sample is disclosed. The method includes: obtaining an image of at least one biological sample; positioning the image of the at least one biological sample; assessing the image of the at least one biological sample; automatically segmenting the image of the at least one biological sample; while simultaneously determining a region of interest of the at least one biological sample; responsive to obtaining the image of the at least one biological sample and a control parameter of the at least one biological sample; and receiving the region of interest and providing an indication of a location of the region of interest.05-27-2010
20080240539Method For Imaging And Differential Analysis Of Cells - Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.10-02-2008
20100128960CORNEA OBSERVATION DEVICE - An object is to allow grasp of a depth position of a corneal image. A cornea observation device 05-27-2010
20080232669METHOD AND/OR SYSTEM FOR ANALYZING BIOLOGICAL SAMPLES USING A COMPUTER SYSTEM - A method and/or system for making determinations regarding samples from biologic sources. A computer implemented method and/or system can be used to automate parts of the analysis.09-25-2008
20100128962VIRTUAL-SLIDE SPECIMEN IMAGE ACQUISITION APPARATUS - The present invention is a virtual-slide specimen image acquisition apparatus that captures images by dividing a specimen into a plurality of sections, having a conveying device in which a plurality of specimens can be arranged and that conveys the plurality of the arranged specimens in a first direction by a distance corresponding to the length of a side along the first direction in one of the divided sections and at first time intervals and an image capturing device that has an image capturing portion for capturing images of the specimens magnified at a predetermined magnification and that scans the specimens conveyed to a predetermined position in a second direction, by a predetermined length at second time intervals, such that the image capturing portion captures images of all of the sections that are positioned identically in the first direction and that are positioned differently in the second direction perpendicular to the first direction.05-27-2010
20090169089Cell Analysis - A system for performing cell population classification in respect of a biological sample. An image is captured in respect of an optical conduit array containing a plurality of cells and signals received therefrom are used to derive a classification scheme defining classes of cells. This scheme is then applied to classify the cells and data representative of the classes and respective locations in the conduit array of the cells.07-02-2009
20080273788System and Method for Quality Assurance in Pathology - Systems and methods for improving quality assurance in pathology using automated quality assessment and digital image enhancements on digital slides prior to analysis by the pathologist are provided. A digital pathology system (slide scanning instrument and software) creates, assesses and improves the quality of a digital slide. The improved digital slide image has a higher image quality that results in increased efficiency and accuracy in the analysis and diagnosis of such digital slides when they are reviewed on a monitor by a pathologist. These improved digital slides yield a more objective diagnosis than reading the corresponding glass slide under a microscope.11-06-2008
20080285837Method for Detecting and Quantitating Multiple-Subcellular Components - A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.11-20-2008
20080273787Automated Selection of Image Regions - A method for automated selection of image regions (“tiles”) in an overall image includes computing a gray-level co-occurrence matrix and entropy of a green plane of the overall image, applying morphological closing to the matrix and thresholding the matrix and entropy image to provide binary masks. The matrix and entropy masks are combined with a vignette mask, the combination indicating areas of acceptable tissue from which tiles are selected randomly. For cancer grading; image data is transformed to Hue, Saturation and Value; for steroid/protein expression analysis it is transformed to cyan and a Sobel of cyan is computed. A feature measure is computed for each tile based on colour and texture, and is carried out randomly but influenced by feature measure. Finally, from the further selection, tiles are chosen which combine high feature measure with low overlap.11-06-2008
20110268342Method for moving cell detection from temporal image sequence model estimation - A computerized robust cell kinetic recognition method for moving cell detection from temporal image sequence receives an image sequence containing a current image. A dynamic spatial-temporal reference generation is performed to generate dynamic reference image output. A reference based object segmentation is performed to generate initial object segmentation output. An object matching and detection refinement is performed to generate kinetic recognition results output. The dynamic spatial-temporal reference generation step performs frame look ahead and the reference images contain a reference intensity image and at least one reference variation image.11-03-2011
20090154788AUTOMATIC SYSTEM FOR JUDGING QUALITY OF CELL - An automatic culture device 06-18-2009
20110206263COUNTING BIOLOGICAL AGENTS ON BIOLOGICAL GROWTH PLATES - The invention is directed to counting techniques for counting biological agents on a biological growth plate or similar medium. In order to automate the counting of biological agents, a biological growth plate is inserted into a biological scanning unit. Upon insertion of the biological growth plate, the biological scanning unit generates an image of the plate. Then, the amount of biological agents that appear in the image, such as a number of bacteria colonies, can be counted or otherwise determined using image processing and analysis routines performed either by the scanning unit or an external computing device, such as a desktop computer, workstation or the like. A variety of counting rules are described herein that can be used to improve the accuracy of automated counts of biological agents on a biological growth plate.08-25-2011
20090028414Data Management in a Linear-Array-Based Microscope Slide Scanner - Systems and methods for processing, storing, and viewing extremely large imagery data rapidly produced by a linear-array-based microscope slide scanner are provided. The system receives, processes, and stores imagery data produced by the linear scanner as a series of overlapping image stripes and combines the data into a seamless and contiguous baseline image. The baseline image is logically mapped into a plurality of regions that are individually addressed to facilitate viewing and manipulation of the baseline image. The system enables dynamic imagery data compression while scanning and capturing new image stripes that eliminates the overhead associated with storing uncompressed image stripes. The system also creates intermediate level images, thereby organizing the baseline image into a variable level pyramid structure referred to as a virtual slide. The system facilitates the use of virtual slides in applications such as telemedicine, telepathology, microscopy education, and analysis of high value specimens like tissue arrays.01-29-2009
20080317324Method and Device for the Characterization of Cells, Clusters and/or Tissue - This invention relates to an apparatus for characterizing cells, cell aggregates and/or tissue with an analysis unit which includes means by which morphological parameters of cells, cell aggregates or tissue are detected and which furthermore includes means by which the detected morphological parameters are evaluated for the purpose of the objective morphological characterization of the cells, cell aggregates or tissue. The invention furthermore relates to a method for objectively characterizing cells, cell aggregates and/or tissue.12-25-2008
20090185734Apparatus and method for analysis of particles in a liquid sample - The invention relates to a measurement apparatus, and method, for analysis of particles in a liquid sample, the apparatus comprising: an image acquiring device, an image analyser, a holder arranged to hold a sample retaining device, and a light refractor positioned between said image acquiring device and said holder; whereby at least one of the image acquiring device, the light refractor and the holder is movable by means of a piezoelectric motor, thereby moving a focus plane within the sample retaining device when it is held by the holder whereby the image acquiring device is adapted to acquire a plurality of images of said sample at different focus planes within the sample retaining device; wherein the image analyser is arranged to analyse at least one acquired image for identifying which of the particles are imaged in focus, and analysing those particles which have been identified as being imaged in focus.07-23-2009
20090034822Methods and Apparatus for Characterising Cells and Treatments - Methods, data processing apparatus and computer program products for characterising cells and the affect of treatments administered to cells are disclosed. In particular methods of identifying bi-nuclear cells are described which include capturing an image of a plurality of marked cells and processing image to obtain features of the plurality of cells. The features are analyzed to determine whether the feature is indicative of bi-nuclear cells. Those cells for which the first feature is indicative of bi-nuclear cells are identified as being bi-nuclear. Three algorithms in particular are described. A first algorithm can be used to determine the number of nuclei in an image of a nuclear component by determining the number of concave regions within the outline of the image. A second algorithm uses a measure of the amount of cytoplasmic material between a pair of nuclei to identify bi-nuclear cells. A third algorithm uses the statistics of the spatial distribution of objects to identify isolated pairs of nuclei which can be considered to be from the same cell.02-05-2009
20130121557SYSTEM AND METHOD FOR AUTOMATED BIOLOGICAL CELL ASSAY DATA ANALYSIS - In one aspect, the present invention relates to a system 05-16-2013
20090103798IMAGE GENERATION - A method of generating a digital representation of a portion of an object from sets of digital data defining the colour content of individually stored, overlapping swathes of pixels of an image of the object portion. The method comprises: 04-23-2009
20090252398Method and System for Creating a Three-Dimensionally-Perceived Image of a Biological Sample - A method and system are provided for enhancing a two-dimensional digital image to render the image as a three-dimensionally perceived image, which is apparent with both monocular and binocular vision. The method, as applied to images of samples captured in a laser-based imaging system (such as a laser scanning cytometry system, for example), produces images with improved spatial resolution, facilitating improvements in both digital and visual analyses. The method comprises offsetting an image by either hardware or data processing techniques, along with additional data processing, including subtraction, scaling, and addition of digital representation of the two-dimensional image.10-08-2009
20080260233AUTOMATED METHOD FOR IMAGE ANALYSIS OF RESIDUAL PROTEIN - A method for measuring residual protein in a cellular specimen including obtaining a low magnification image of a candidate object of interest, filtering the candidate object of interest pixels with a low pass filter, morphologically processing the candidate object of interest pixels to identify artifact pixels, identifying the candidate object of interest by eliminating artifact pixels, acquiring a higher magnification image of the subsample for the candidate object of interest, transforming pixels of the higher magnification image in a first color space to a second color space to differentiate higher magnification candidate object of interest pixels from background pixels, identifying an object of interest from the candidate object of interest pixels in the second color space, and determining the optical density of the protein in a cell contained in a subsample, wherein the optical density is indicative of the residual component of a cellular protein.10-23-2008
20090245611Method and Apparatus for Removing Tissue Autofluorescence - Techniques for removing image autoflourescence from fluorescently stained biological images are provided herein. The techniques utilize non-negative matrix factorization that may constrain mixing coefficients to be non-negative. The probability of convergence to local minima is reduced by using smoothness constraints. The non-negative matrix factorization algorithm provides the advantage of removing both dark current and autofluorescence.10-01-2009
20090245610Method and Apparatus for Detecting Irregularities in Tissue Microarrays - The present techniques provide systems and methods for registering images of tissue spots on a tissue microarray (TMA). In studies involving multiple biomarkers being studied on the same TMA, the TMA slide is removed from the microscope, stained, and then imaged, often multiple times. The present techniques relate to validation of the registration of the acquired images of the same TMA. An automatic approach to register the images and detect registration failures as provided herein may enhance the rapid analysis of the tissues. Artifacts such as tissue folding and tissue loss are also determined automatically.10-01-2009
20090316977SYSTEMS AND METHODS FOR ANALYZING WORKFLOW ASSOCIATED WITH A PATHOLOGY LABORATORY - A computer system for analyzing a specimen processing workflow in a pathology laboratory including a display and a processor configured to provide a first interface, a second interface, and a third interface to the display, receive, via the first interface, a first set of parameters associated with an existing specimen processing workflow in a pathology laboratory, the first set of parameters including a workflow process and a workflow scale, and determine, based on the first set of parameters, current performance data of the existing specimen processing workflow having an associated current cost information and current time utilization. The processor may further be configured to receive, via the second interface, a second set of parameters associated with a proposed modification to the existing specimen processing workflow, the second set of parameters including parameters associated with at least one laboratory device, determine, based on the first and second sets of parameters, revised performance data having an associated revised cost information and revised time utilization, and provide an output comprising a comparison between revised performance data and the current performance data.12-24-2009
20090257640CLASSIFICATION OF SAMPLES - Methods disclosed herein include: (a) determining positions of a plurality of cells based on one or more images of the cells; (b) for at least some of the plurality of cells, generating a matrix that includes two-dimensional information about positions of neighboring cells, and determining one or more numerical features based on the information in the matrix; and (c) classifying the at least some of the plurality of cells as belonging to at least one of multiple classes based on the numerical features.10-15-2009
20120195488Methods and Compositions Related to Cellular Assays - The present invention relates generally to the fields of molecular biology and cancer. More specifically, the invention concerns methods and compositions useful for diagnosing and assessing a subject's response to therapy.08-02-2012
20090316976Complexity-based analysis of cellular flickering - Provided herein are methods and systems for assessing cellular flickering. The methods include using a phase contrast microscope to obtain a plurality of images of a cell, digitizing and/or pixilating the plurality of images such that the plurality of images are segmented into an array of pixels, each pixel representing a portion of the cell, and measuring the fluctuations in the pixel intensities. The methods further include calculating a complexity measure for the individual pixels based on the measured fluctuations of the portions of the cell represented by the individual pixels. Such complexity measures can then be mapped and/or plotted in order to assess cellular flickering, and thereby assess biological function and other characteristics of the cell.12-24-2009
20120063664INERTIAL PARTICLE FOCUSING FLOW CYTOMETER - A flow cytometry system includes an inertial particle focusing device including a plurality of substantially parallel microchannels formed in a substrate, each microchannel having a width to height ratio in the range of 2:3 to 1:4, an analyzer disposed adjacent the inertial particle focusing device such that the analyzer is configured to detect a characteristic of particles in the inertial particle focusing device, and a controller connected to the analyzer and configured to direct the detection of the characteristic of the particles.03-15-2012
20090116723MODULE FOR DETECTING POORLY DIFFERENTIATED CANCERS AND PATHOLOGICAL IMAGE DIAGNOSIS SUPPORT APPARATUS, PROGRAM AND RECORDING MEDIUM INCLUDING THE SAME - The detection accuracy of poorly differentiated cancers in adenocarcinoma is improved by restricting false detection. Cell nucleus detection means 05-07-2009
20100150423Multi-nucleated cell classification and micronuclei scoring - Methods and systems for counting nuclei for cells in a cell-containing sample are disclosed, such as carried out on a computer. The methods comprise: receiving a raw image of the cell-containing sample; transforming the raw image into a segmented image comprising one or more nuclei clusters. The methods further comprise: for each of the one or more nuclei clusters, obtaining a convex hull of the nuclei cluster; locating any indentations on the nuclei cluster by comparing the nuclei cluster to the convex hull of the nuclei cluster; calculating a first nuclei count based on a tally of the indentations; and assigning the nuclei cluster to a cell among the plurality of cells. The methods further comprise: calculating a second nuclei count for each cell by totaling the first nuclei counts of its constituent nuclei clusters; and presenting a result based on the second nuclei count for at least one of the plurality of cells.06-17-2010
20120063663MEDICAL IMAGE PROCESSING APPARATUS AND MEDICAL IMAGE PROCESSING METHOD - A medical image processing apparatus includes a vessel running data generating unit, a lesion site detecting unit, a vessel dominant region data generating unit, a lesion site dominant region data generating unit, and a diagnostic image data generating unit. The vessel running data generating unit generates vessel running data of a vessel. The lesion site detecting unit detects positional information of a lesion site in the vessel. The vessel dominant region data generating unit generates vessel dominant region data indicating a region dominated by the vessel in a region provided with nutrition. The lesion site dominant region data generating unit generates lesion site dominant region data indicating a region dominated by the lesion site in the region provided with nutrition. The diagnostic image data generating unit generates diagnostic image data by superimposing the vessel dominant region data and the lesion site dominant region data on morphological image data.03-15-2012
20100254590SYSTEM AND METHODS FOR RAPID AND AUTOMATED SCREENING OF CELLS - A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known to methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.10-07-2010
20090116724EVALUATION SYSTEM, EVALUATION METHOD, AND RECORDING MEDIUM - An evaluation system includes a capture unit for capturing an image of a living tissue in which HER2 protein and cell nucleuses are dyed, a discrimination unit for identifying a cell membrane from the image of the living tissue based on dyed cell nucleuses within the image of the living tissue captured by the capture unit to discriminate a dyed state of the cell membrane, and an evaluation unit for evaluating development of the HER2 protein based on a discrimination result by the discrimination unit.05-07-2009
20090097733METHOD AND APPARATUS FOR CLUSTERING AND VISUALIZATION OF MULTICOLOR CYTOMETRY DATA - A computer implemented method is provided for clustering and visualization of an n-dimensional space in a Euclidean space. The method includes: collecting a plurality of multi-dimensional data sets; estimating a probability density function from each data set; approximating a dissimilarity between every pair of said probability density functions and recording every dissimilarity in a dissimilarity matrix; embedding the dissimilarity matrix into a Euclidean space having a dimensionality of three or less using a multi-dimensional scaling method; and graphically displaying relationships between data sets using data in the Euclidean space on a display of a computing device.04-16-2009
20100177950Systems and methods for treating, diagnosing and predicting the occurrence of a medical condition - Clinical information, molecular information and/or computer-generated morphometric information is used in a predictive model for predicting the occurrence of a medical condition. In an embodiment, a model predicts risk of prostate cancer progression in a patient, where the model is based on features including one or more (e.g., all) of preoperative PSA, dominant Gleason Grade, Gleason Score, at least one of a measurement of expression of AR in epithelial and stromal nuclei and a measurement of expression of Ki67-positive epithelial nuclei, a morphometric measurement of average edge length in the minimum spanning tree (MST) of epithelial nuclei, and a morphometric measurement of area of non-lumen associated epithelial cells relative to total tumor area. In some embodiments, the morphometric information is based on image analysis of tissue subject to multiplex immunofluorescence and may include characteristic(s) of a minimum spanning tree (MST) and/or a fractal dimension observed in the images.07-15-2010
20090297016CLASSIFYING IMAGE FEATURES - Methods are disclosed for classifying different parts of a sample into respective classes based on an image stack that includes one or more images.12-03-2009
20100232674CELL-IMAGE ANALYZING APPARATUS - A cell-image analyzing apparatus includes: a cell imaging system having an imaging optical system and an image sensor, for imaging cells that exist in a vessel; a cell-image analyzer for automatically analyzing a predetermined characteristic quantity on the cells using a cell image captured via the cell imaging system, upon delimiting cell regions; and a cell-contour emphasizing system for automatically emphasizing contour portions of images of the cells that exist in the vessel, which is arranged at a shot position of the cell imaging system.09-16-2010
20130216118Centromere Detector and Method for Determining Radiation Exposure From Chromosome Abnormalities - A method for determining radiation exposure from chromosome abnormalities present in a specimen by determining the location or locations of the centromere of each chromosome in a cell in an image of a metaphase cell by segmentation of an accurately drawn chromosome centerline followed by selection of a longitudinal cross-section with the minimum width or intensity or width and intensity; counting the number of centromeres in each chromosome in each cell; computing the frequency of dicentric chromosomes in a population of cells; and determining the radiation dose by comparing the computed frequency of dicentric chromosomes with a previously determined dose-response curve from a calibrated source.08-22-2013
20110058728Device and Method for Automatic Detection of Dynamic Processes of Cells in Cell Samples - A device for detecting dynamic processes of cell samples has a camera and a data processing system with a first module recognizing the cells of a first image, a second module allocating an identity to the cells, a third module allocating a frame to cells of the first image with one cell in the frame. First, second and third modules are serially connected. A fourth module recognizes cells within frames and allocates identities of cells of the subsequent image and determines similarity of cells relative to cells detected in predecessor images; cells most similar to predecessor cells are assigned as successors; newly added cells are assigned new identities. A fifth module allocates frames for cells of the subsequent image with one cell in the frame. Fourth and fifth modules are a loop for images following the first image. Data memory and data display for images with recognized cells are provided.03-10-2011
20100215245CALIPER FOR MEASURING OBJECTS IN AN IMAGE - The invention relates to a user interface (08-26-2010
20100119141Method for discovering relationships in data by dynamic quantum clustering - Data clustering is provided according to a dynamical framework based on quantum mechanical time evolution of states corresponding to data points. To expedite computations, we can approximate the time-dependent Hamiltonian formalism by a truncated calculation within a set of Gaussian wave-functions (coherent states) centered around the original points. This allows for analytic evaluation of the time evolution of all such states, opening up the possibility of exploration of relationships among data-points through observation of varying dynamical-distances among points and convergence of points into clusters. This formalism may be further supplemented by preprocessing, such as dimensional reduction through singular value decomposition and/or feature filtering.05-13-2010
20090074284SYSTEM AND METHOD FOR CREATING MAGNIFIED IMAGES OF A MICROSCOPE SLIDE - A method and system for creating a digital, virtual slide having optimum image quality characteristics. Multiple regions of a physical slide are identified as well as at least two focus z-positions z03-19-2009
20090074283SYSTEM AND METHODS FOR RAPID AND AUTOMATED SCREENING OF CELLS - A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.03-19-2009
20090074282METHODS AND SYSTEM FOR VALIDATING SAMPLE IMAGES FOR QUANTITATIVE IMMUNOASSAYS - A method and system for automatically evaluating quality of a slide-mounted tissue sample includes receiving a digital image of a magnified portion of the slide-mounted tissue sample. At least one quantitative quality indicator is automatically determined for at least one of the samples, and the digital image of the magnified portion of the sample. Each of the quantitative quality indicators is automatically compared to a respective minimum acceptable quality threshold. The quantitative quality indicators and associated quality thresholds are selected for suitability with an automated quantitative immunoassay. Failure of one or more of the quantitative quality indicators to meet its respective minimum acceptable quality threshold suggests that the sample is unsuitable for subsequent automated pathological evaluation. Results can be examined at a user interface allowing for user inspection of samples determined to be unsuitable, the user interface also having provisions for manual override of the determination.03-19-2009
20110026803METHODS AND SYSTEMS FOR DIGITALLY ENHANCING AN IMAGE OF A STAINED MATERIAL - Methods and systems for digitally enhancing an initial image of a material to which a plurality of stains were previously applied, that generally comprise: unmixing the image into a plurality of individual reconstructed images, each individual image corresponding to one of the stains; estimating a residual image corresponding to the difference between the original image and the reconstructed images; adjusting one or more components of the individual images; mixing the adjusted components using one or more estimated mixing coefficients; and adding the residual image to the mixed adjusted components to generate an enhanced image.02-03-2011
20090324051Image Classifier Training - Methods are disclosed that include: (a) applying a first stain to a first sample having a plurality of regions, where the first stain selectively binds to only a first subset of the regions of the first sample; (b) applying a second stain to the first sample, where the second stain binds to a second set of regions of the first sample; (c) obtaining an image of the first sample, and analyzing the image to obtain a first component image corresponding substantially only to spectral contributions from the first stain, and a second component image corresponding substantially only to spectral contributions from the second stain; and (d) training a classifier to identify regions of a second sample based on information derived from the first and second component images, the identified regions corresponding to the first subset of regions of the first sample.12-31-2009
20110110577MATHEMATICAL IMAGE ANALYSIS BASED CELL REPROGRAMMING WITH APPLICATIONS FOR EPIGENETIC AND NON-EPIGENETIC BASE INDUCED PLURIPOTENT STEM CELL DERIVATION - Mathematical and statistical image analysis methods and systems are applied to enhance and refine the process of reprogramming cells, for example, to modify cells from patients into custom-matched stem cells.05-12-2011
20100322502MEDICAL DIAGNOSIS SUPPORT DEVICE, IMAGE PROCESSING METHOD, IMAGE PROCESSING PROGRAM, AND VIRTUAL MICROSCOPE SYSTEM - The present invention provides a medical diagnosis support device, which is capable of acquiring information to support medical diagnosis for easily and precisely determining chromosome abnormality and/or gene amplification related to cancer or genetic disorder.12-23-2010
20090034823Compartment segregation by pixel characterization using image data clustering - The present invention relates generally to improved methods of defining areas or compartments within which biomarker expression is detected and quantified. In particular, the present invention relates to automated methods for delineating marker-defined compartments objectively with minimal operator intervention or decision making. The method provides for precise definition of tissue, cellular or subcellular compartments particularly in histological tissue sections in which to quantitatively analyzing protein expression.02-05-2009
20110019897CELL-IMAGE ANALYZING APPARATUS - A cell-image analyzing apparatus is intended to analyze, using a cell image, a cell collective that forms a colony, and is provided with a computer. The cell-image analyzing apparatus has an image analysis software that makes the computer function as a boundary element extracting means for extracting boundary elements of subjects upon analyzing the cell image; a possible colony region determining means for determining, as a possible colony region, a region surrounded by boundary elements of subjects and having a size greater than a first criterion value; and a colony region determining means for determining, in the possible colony region, a region containing more than a predetermined number of clustered regions each being surrounded by boundary elements of subjects and having a size smaller than a second criterion value, as a colony region.01-27-2011
20110044527Methods and Algorithms for Cell Enumeration in a Low-Cost Cytometer - The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.02-24-2011
20090214101Targeted Nanostructures for Cellular Imaging - Compositions and methods related to targeted carbon nanostructures. More particularly, targeted carbon nanostructures comprising: a C08-27-2009
20090097734CELLULAR THICKNESS MEASUREMENT METHOD - To enable precise measurement of a cellular thickness distribution regardless of changes in the refractive index of cell(s). Two-dimensional distribution of phase information is obtained, in a state where a first culture solution having a first refractive index is stored so as to completely immerse cells adhered onto a bottom face of a culture vessel, by transmitting light of a wavelength, and by photographing the transmitted light. Next, two-dimensional distribution of phase information is obtained, in a state where a second culture solution having a second refractive index is stored to have a depth to completely immerse the cells in the culture vessel, by transmitting light, and by photographing the transmitted light. Next, respective average values of the phase information are calculated. Next, the refractive index of the cell is estimated. Next, the thickness dimension is calculated using the refractive index.04-16-2009
20090245612AUTOMATED IMAGE ANALYSIS - One automated imaging process, as described herein, includes: a) obtaining digital images of objects in a biological sample; b) selecting a plurality of objects of interest from the digital images; c) obtaining multiple images of the selected objects of interest at a plurality of different wavelengths; d) combining one of said multiple images with a corresponding digital image to produce a combined image; and e) analyzing the combined image in order to characterize the biological sample.10-01-2009
20100014741METHODS AND APPARATUS RELATED TO GATE BOUNDARIES WITHIN A DATA SPACE - In one embodiment, one or more processor-readable media storing code representing instructions that when executed by one or more processors cause the one or more processors to receive a set of parameter values defining a boundary within a data space associated with a dataset. The dataset can represent signaling related to a test substance. A first metric can be defined based on a first portion of the dataset associated with a first region defined by the boundary. A second metric can be defined based on a second portion of the dataset associated with a second region defined by the boundary after the boundary is modified. The second region can be different than the first region.01-21-2010
20090087074METHODS AND SYSTEMS FOR PROCESSING BIOLOGICAL SPECIMENS UTILIZING MULTIPLE WAVELENGTHS - Methods, systems and computer readable media for processing one or more biological specimens carried by specimen slides. Images of objects in a specimen are acquired and objects of interest in the acquired images are identified. Additional images of identified objects of interest may be acquired at multiple wavelengths. Cellular features of objects of interest are extracted from images and may be used for classifying the specimen, e.g., as normal or suspicious/abnormal, based a probabilistic model that utilizes the extracted features.04-02-2009
20120243769QUANTIFYING CELL DEATH - The invention relates to methods of diagnosis, particularly methods of staging and diagnosing neurodegenerative diseases using images of cell death in the eye.09-27-2012
20090028413SYSTEM AND METHOD EMPLOYING PHOTOKINETIC TECHNIQUES IN CELL BIOLOGY IMAGING APPLICATIONS - A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.01-29-2009
20110249883METHODS FOR SEGMENTING OBJECTS IN IMAGES - A method for segmenting a digital image into a plurality of target objects, comprising, generating a plurality of probability maps of the image, wherein each probability map is derived from a different segmentation classifier; generating a combined probability map based on the plurality of probability maps; mapping a plurality of image points based on one or more local object maxima; applying one or more object constraints based at least in part on the mapped points to identify local object information; applying one or more regional thresholds to the combined probability map, given the local object information and a background mask, to segment the image into regions; creating a segmented image at least in part by merging the segmented regions with corresponding local object maxima; and at least temporarily storing or displaying the segmented image on a digital device.10-13-2011
20120148142DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY - Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.06-14-2012
20100296718SOFT TISSUE SEGMENTATION USING A BONE ATLAS - A method of segmenting one or more soft tissue organs in a 3-D medical image, the method comprising: 11-25-2010
20080317325DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY - Photometric and morphometric features derived from multi-mode imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.12-25-2008
20100260406METHODS AND SYSTEMS FOR IMAGING CELLS - The invention generally provides methods and systems for determining characteristics of cellular structures. The methods include non-invasive, non-perturbing, automatable, and quantitative methods and may be applied to the examination of cells such as stem cells, embryos, and egg cells.10-14-2010
20090022388METHOD AND APPARATUS FOR ANALYZING CLUSTERS OF OBJECTS - The present invention relates to a method and an apparatus for processing and metrically quantifying images of objects containing clusters of points/spots, such as biological specimens comprising cluster of cells, in particular of human or animal origin, or images thereof. In particular, the present invention relates to a method for processing images of irregularly shaped objects in the form of at least one cluster of punctiform or spot-shaped objects, comprising a stage of acquisition of a digital image of said objects, a stage of image elaboration (IMA-EL) for quantizing said digital image to 1 bit and a stage of metrical processing of said 1-bit quantized image, wherein said stage of metrical processing comprises a stage of object's metrical quantification (QUANT) that on its turn comprises: 01-22-2009
20080247627Apparatus and Method For Detecting Protein Crystals - A protein crystal detecting method for detecting a protein crystal in protein solution held in a crystallizing container, wherein layer images are obtained by imaging cross sections of a protein solution, a crystal feature image for each layer is created by extracting a feature portion of a protein crystal from the layer images, judgment parameters such as a representative number of pixels representing the number of pixels of the layer image showing the largest feature portions, a representative average number of pixels for each label, and a layer-to-layer rate.10-09-2008
20120201445COMPUTER AIDED DIAGNOSTIC SYSTEM INCORPORATING APPEARANCE ANALYSIS FOR DIAGNOSING MALIGNANT LUNG NODULES - A computer aided diagnostic system and automated method diagnose lung cancer through modeling and analyzing the visual appearance of pulmonary nodules. A learned appearance model used in such analysis describes the appearance of pulmonary nodules in terms of voxel-wise conditional Gibbs energies for a generic rotation and translation invariant second-order Markov-Gibbs random field (MGRF) model of malignant nodules with analytically estimated characteristic voxel neighborhoods and potentials.08-09-2012
20080304732AUTOMATIC CHARACTERIZATION OF CELLULAR MOTION - The present techniques provide for the evaluation of cellular motion and/or cellular properties based on an analysis of motion. In an exemplary technique, images of one or more cells are acquired and motion data for the one or more cells is derived from the images. The motion data is decomposed to generate one or more motion components. The one or more motion components can be used to evaluate cellular properties and/or cellular motion properties.12-11-2008
20080304733Apparatus and Methods for Automated Assessment of Tissue Pathology - Methods and apparatus for assessing tissue pathology involve computing an index having a value based upon measures of a plurality of morphological features of cell nuclei in the tissue. The methods may be performed completely automatically or semi-automatically. The index value can be predictive of outcome. The index value may be determined by computing discriminant scores for the cell nuclei based upon values of the measures of morphological features and classifying the nuclei into bins based upon the discriminant score values. The index may be based upon proportions of the nuclei classified in different ones of the bins.12-11-2008
20080267485Medical Image Processing Apparatus for Analyzing Properties of Living Body Based on Image Data Thereof - A medical image processing apparatus for analyzing properties of living body based on image data of the living body is provided. The medical image processing apparatus executes a predetermined image analysis processing on the image data of the living body, to obtain shape image data obtained by extracting a profile of the living body, and pattern image data obtained by extracting the pattern on the interior of the profile of the living body. A fractal dimension D10-30-2008
20080253641Method for Drug Screening and Characterization by Calcium Flux - The instrumentation and methods described herein are based on imaging and measuring single cell dose response by fluorescent ion imaging that records live cell responses to drug doses with no apparent damage to the cells or toxic side effects. Dose response curves and other pharmacological parameters can be computed by imaging and measuring oscillation changes for each dose and each cell. Large variability of cellular responses under the same experimental conditions indicates that pharmacological benchmarks are determined by statistical distribution of cells and cellular physiology. The instrumentation and methods described herein provide a means of measuring statistical variability of cell populations for improved screening and development methods for drugs.10-16-2008
20080205740Method of Analyzing Cell Structures and Their Components - A cell is provided that contains a plurality of virus particles. A first image of a first virus particle and a second image of a second virus particle are taken by electron microscopy technology. The first virus particle is characterized as being in a first maturity stage and the second virus particle as being in a second maturity stage. The first image and the second image are transformed to first and second gray scale profiles, respectively, based on pixel data. The first and second gray scale profiles are then saved as first and second templates, respectively. A third virus particle in a third image is identified. The third image is transformed into a third gray scale profile. The third gray scale is compared to the first and second template to determine a maturity stage of the third virus particle.08-28-2008
20100329535Methods, Systems and Computer Program Products for Analyzing Histology Slide Images - Methods, systems and computer program products for normalizing histology slide images are provided. A color vector for pixels of the histology slide images is determined. An intensity profile of a stain for the pixels of the histology slide images is normalized. Normalized image data of the histology slide images is provided including the color vector and the normalized intensity profile of a stain for the pixels of the histology slide images.12-30-2010
20100329536IMAGE ANALYSIS OF BIOLOGICAL OBJECTS - Systems and methods are described for performing image analysis. A computer-implemented method for analyzing images may include quantitatively analyzing image data to identify image objects relative to a background portion of the image according to predefined object criteria, the image data including a plurality of image objects that represent objects in a sample distributed across a substrate. The identified image objects are further clustered into groups or colonies of the identified image objects according to predetermined clustering criteria.12-30-2010
20110075914SYSTEM AND METHOD FOR THE QUANTITATIVE ASSESSMENT OF DIGITAL HISTOLOGY IMAGES - The present disclosure concerns a method and system for assessing image quality of sub-images used to form a composite image using an algorithm developed by applying statistical correlation techniques to historical data on features of sub-images which were manually evaluated by experts and using those assessment to make decisions about the further processing of the sub-images which are being assessed. The method and system find particular value in the processing of sub-images generated while there is relative motion between the specimen under examination and the objective lens of a microscope such as when the microscope stage follows a planned traverse in the focal plane and a digital image is created at intervals correlated to the motion of the stage so that a region of interest in the specimen under examination is covered by the sub-images. The further processing decisions include manually examining the sub-images assessed to have unacceptable image quality, reimaging the entire region of interest and recreating specific sub-images. The method and system may also involve overlaying portions of a composite image with an indication that they were drawn from sub-images of unacceptable image quality.03-31-2011
20110096976AUTOMATED METHOD AND SYSTEM FOR SETTING IMAGE ANALYSIS PARAMETERS TO CONTROL IMAGE ANALYSIS OPERATIONS - A method and system for setting image analysis parameters to control image analysis operations. The method and system include collecting set of digital training images including a set of states for the set of digital training images. An objective function is defined to determine a relative quality of plural different parameter sets used for digital image analysis. Values for the plural different parameter sets that maximize (or minimize) the objective function are determined. The method and system increases a usability of high content screening technologies by reducing a required level of expertise required to configure digital image processing.04-28-2011
20110096977AUTOMATED METHOD AND SYSTEM FOR SETTING IMAGE ANALYSIS PARAMETERS TO CONTROL IMAGE ANALYSIS OPERATIONS - A method and system for setting image analysis parameters to control image analysis operations. The method and system include collecting set of digital training images including a set of states for the set of digital training images. An objective function is defined to determine a relative quality of plural different parameter sets used for digital image analysis. Values for the plural different parameter sets that maximize (or minimize) the objective function are determined. The method and system increases a usability of high content screening technologies by reducing a required level of expertise required to configure digital image processing.04-28-2011
20110096975SYSTEMS AND METHODS FOR IDENTIFYING MICROPARTICLES - Disclosed are systems and methods for identifying microparticles or features arranged in high density arrays. Using the techniques of the present teachings allow for effective discrimination and characterization of microparticles such as sequencing beads or features having densities of about 39×1004-28-2011
20090034824Computerized image analysis for acetic acid induced Cervical Intraepithelial Neoplasia - A method for differentiating cancerous lesions from surrounding tissue, which includes extracting an opacity parameter from acetowhite regions of pre acetic acid and post acetic acid images of a cervix.02-05-2009
20110052038TISSUE-SLICE IMAGE ACQUIREMENT AND DISPLAY APPARATUS, TISSUE-SLICE IMAGE ACQUIREMENT AND DISPLAY METHOD, AND TISSUE-SLICE IMAGE ACQUIREMENT AND DISPLAY PROGRAM - A tissue-slice image acquirement and display apparatus includes: an entire-image acquirer that acquires a bright-field image of an entirety of a first slice of a tissue and a dark-field image of an entirety of a second slice of the tissue; a modifier that modifies a profile shape of a slice portion in the dark-field image on the basis of a profile shape of a slice portion in the bright-field image; a magnified-image acquirer that determines a range encompassing the second slice on the basis of the profile shape of the modified slice portion and that acquires, in a dark field, a magnified image of the second slice in the determined range; and a display controller that causes the bright-field image to be displayed and that causes a portion of the magnified image, the portion corresponding to a position selected in the bright-field image, to be displayed.03-03-2011
20110052037Methods and Algorithms For Cell Enumeration in a Low-Cost Cytometer - The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.03-03-2011
20100166287Neuronal Profiling - The present invention provides automated methods for cell body extension analysis, software for carrying out such methods, and detection devices comprising such software.07-01-2010
20100067774METHODS OF CHROMOGEN SEPARATION-BASED IMAGE ANALYSIS - Methods for chromogen separation-based image analysis are provided, with such methods being directed to quantitative video-microscopy techniques in cellular biology and pathology applications.03-18-2010
20100067775METHODS OF CHROMOGEN SEPARATION-BASED IMAGE ANALYSIS - Methods for chromogen separation-based image analysis are provided, with such methods being directed to quantitative video-microscopy techniques in cellular biology and pathology applications.03-18-2010
20090175529METHOD AND APPARATUS FOR SELECTIVELY TARGETING SPECIFIC CELLS WITHIN A CELL POPULATION - This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.07-09-2009
20110142324METHOD OF RECONSTITUTING CELLULAR SPECTRA USEFUL FOR DETECTING CELLULAR DISORDERS - Embodiments of the present disclosure provides improved methods for determining the presence of abnormalities in exfoliated cells. In one embodiment, the present disclosure provides methods for reconstructing cellular spectrum of a cell sample by creating a spectral map of the cellular sample, generating a binary mask of the spectral map, removing edge artifacts from each cell, and co-adding spectral data of each pixel corresponding to the cell to reconstruct the spectrum of each cell.06-16-2011
20100034453DETECTION OF RNA IN TISSUE SAMPLES - Methods of identifying microRNA labeled with a fluorescent probe in a biological sample are disclosed. The methods include obtaining a plurality of fluorescence images of the sample and forming an image stack based on the plurality of fluorescence images, decomposing the image stack to obtain a component image that corresponds to the labeled microRNA, and identifying the microRNA based on the component image.02-11-2010
20100021037METHOD AND SYSTEM FOR IDENTIFYING BIOLOGICAL SPECIMEN SLIDES USING UNIQUE SLIDE FINGERPRINTS - Method and system for processing specimen slides. A first image of a unique feature, such as a fiducial mark, of a specimen slide is acquired, and a second image of the same region of a fiducial mark is acquired at a later time. The first and second fiducial mark images are compared in order to determine whether they are images of the same fiducial mark of the same specimen slide, thereby allowing the system to detect when one specimen slide has been swapped with another specimen slide. The comparison may also involve images of multiple features on the specimen carrier, e.g., sets of multiple fiducial marks.01-28-2010
20120148141COMPACT AUTOMATED SEMEN ANALYSIS PLATFORM USING LENS-FREE ON-CHIP MICROSCOPY - A compact and light-weight lens-free platform to conduct automated semen analysis is disclosed. The device employs holographic on-chip imaging and does not require any lenses, lasers or other bulky optical components to achieve phase and amplitude imaging of sperm a relatively large field-of-view with an effective numerical aperture of approximately 0.2. A series of digital image frames is obtained of the sample. Digital subtraction of the consecutive lens-free frames, followed by processing of the reconstructed phase images, enables automated quantification of the count, the speed and the dynamic trajectories of motile sperm, while summation of the same frames permits counting of immotile sperm.06-14-2012
20120148140METHOD AND DEVICE FOR MULTI-PARAMETER IMAGING WITHIN A SINGLE FLUORESCENT CHANNEL - A method of monitoring temporal and spatial information of cells includes trapping a plurality of cells within single cell traps contained in a microfluidic device having an inlet, and an outlet. A first fluorescent stain specific to a first target is flowed into the inlet of the device and exposed to the trapped cells. The trapped cells are then imaged as a function of time. A fluorescent stain specific to a different target is flowed into the inlet of the device, the subsequent fluorescent stain having an emission spectrum that substantially overlaps with the emission spectrum of the prior fluorescent stain. The plurality of trapped cells are then imaged again as a function of time. The process can be repeated with additional fluorescent stains having substantially overlapping emission spectra. Images may be subtracted to reveal the contribution of a single fluorescent stain.06-14-2012
20100002929Image-based methods for measuring global nuclear patterns as epigenetic markers of cell differentiation - The invention provides methods for determining the differentiation state of cells. The methods include non-invasive, non-perturbing, automatable, and quantitative methods of analysis of cell colonies, individual cells, and/or cellular structures.01-07-2010
20120099779 METHOD AND SYSTEM FOR SEGMENTING A BRAIN IMAGE - A method is proposed for segmenting a brain image into a CSF region, a WM region and a GM region. An upper limit for the intensity values of a CSF region in the image is estimated such that the points of the image having an intensity less than this upper limit include a subset of the points which form a spatially connected group and which have a peaked intensity distribution. In other words, the invention exploits both the expected spatial distribution and expected intensity distribution of the CSF region. This makes it possible for the method to provide reliable discrimination of the CSF region even in CT images with poor image quality. Various methods are proposed for using the upper limit, and for improving the segmentation accuracy.04-26-2012
20110091091PROCESS AND SYSTEM FOR ANALYZING THE EXPRESSION OF BIOMARKERS IN CELLS - The invention relates generally to a process of analyzing and visualizing the expression of biomarkers in individual cells wherein the cells are examined to develop patterns of expression by using a grouping algorithm, and a system to perform and display the analysis04-21-2011
20110064296IMAGING SYSTEM WITH DYNAMIC RANGE MAXIMIZATION - A method of operating an instrument that is a macroscope, microscope, or slide scanner is provided where the instrument has a larger dynamic range for measurement than a dynamic range required in the final image of a specimen. In the method, data is measured from a specimen using the instrument, and the dynamic range of the measured data is contracted in the final image file during scanning.03-17-2011
20110317904IMAGE PROCESSING METHOD FOR A MICROSCOPE SYSTEM - An embodiment is disclosed for performing the image processing for analyzing the results of a fluorescence in situ hybridization (FISH) microscopic automated sample analysis to determine specific chromosomal characteristics.12-29-2011
20110317903Method and Apparatus for Segmenting Biological Cells in a Picture - A method of segmenting biological cells in a picture so that the biological cells represent a foreground of the picture includes a step of applying a first fast marching algorithm to the picture or to a pre-processed version of same in order to obtain a first fast marching image. In addition, the method includes a step of segmenting the first fast marching image or a further-processed version of same into a plurality of homogeneous regions. Furthermore, the method includes a step of mapping each of the homogeneous regions to one node of a graph, respectively. In addition, the method includes a step of classifying each homogeneous region either as background or foreground on the basis of the graph. Moreover, the method includes a step of applying a second fast marching algorithm within the homogeneous regions classified as foreground so as to segment the foreground into individual biological cells.12-29-2011
20120114217TIME RESOLVED DIGITAL SUBTRACTION ANGIOGRAPHY PERFUSION MEASUREMENT METHOD, APPARATUS AND SYSTEM - A method is disclosed of providing time dependent three dimensional imaging of a region of a patient comprising blood vessels in a perfusion bed.05-10-2012
20120207376Bioinspired System for Image Processing - A method for digital image processing is bioinspired and includes an architecture that emulates the functions of photoreceptors, horizontal cells, bipolar cells and ganglion cells of a primate retina based on an image as input. The method detects edges and properties of the surfaces present in the digital image. The output is a data set that includes photoreceptor emulators that emulate photoreceptor cells and connected to the data input. Each emulator includes a cellular base structure with a modulated data input, a calculation center to process the modulated data and an output of the data processed by the calculation center, and the emulators forming a virtual retina in which each emulator is parameterized.08-16-2012
20090129660Automated Detection of Cell Colonies and Coverslip Detection Using Hough Transforms - The present invention provides methods and systems for automatic detection of the location of cell colonies on a specimen slide, in particular under the coverslip of a specimen slide. Slide scanning can be performed using an automated microscope with motorized axes. The location of the colonies can be determined by image analysis, which is followed by automatically finding metaphase cells and associating them with each colony. The invention also provides an automated, Hough-transform-based method for identifying the location of the slide coverslip and, if desired, analyzing only the image area contained within the coverslip.05-21-2009
20120207375Bioinspired System for Processing and Characterising Colour Attributes of a Digital Image - A method of processing color attributes of digital images is bioinspired and includes an architecture that emulates the functions of the retina of a primate based on an image as input. The method detects the color attributes in the image. The output is a data set that includes emulators that a virtual retina in which each emulator is parameterized and in which there are emulators of type G ON and G OFF midget ganglion cells, emulators of bistratified ganglion cells, and emulators of type R ON and OFF midget ganglion cells each connected to a plurality of type R ON and OFF midget bipolar cell emulators which in turn are connected through horizontal cell emulators, to a plurality of type L cone photoreceptor emulators and to a plurality of horizontal emulators and generates a third colour channel (A) of output signals.08-16-2012
20100061618METHODS OF CHROMOGEN SEPARATION-BASED IMAGE ANALYSIS - Methods for chromogen separation-based image analysis are provided, with such methods being directed to quantitative video-microscopy techniques in cellular biology and pathology applications.03-11-2010
20090297015Method for Detecting Contours in Images of Biological Cells - A method for identifying membrane contours in images of biological cells is described and comprises the following steps: 12-03-2009
20120014588MEDICAL IMAGE DIANOSTIC DEVICE, REGION-OF-INTERST SETTING METHOD, AND MEDICAL IMAGE PROCESSING DEVICE - The medical image diagnosis device of the invention includes image generating means configured to obtain image data of a tissue of an object and generate an image of the tissue of the object based on the image data, calculation means configured to calculate at least one of brightness and motion vectors of plural measurement points of the generated image, input means configured to specify an observation region of the image, a database in which a characteristic amount of at least one of the brightness and the motion vectors of the measurement points in respective images of plural different observation regions is set and stored in advance, checking means configured to read the characteristic amount of the observation region that is specified through the input means from the database and check the characteristic amount with results of calculation performed on the generated image by the calculation means, and ROI setting means configured to set a region of interest in the generated image based on checked results of the checking means.01-19-2012
20120014587IMAGE PROCESSING APPARATUS AND METHOD - An image processing apparatus includes the following elements. A specifying unit specifies a blank region of an image of image data by using coefficient data which is decoded from encoded data, the encoded data being obtained by encoding coefficient data generated from the image data. A dividing unit divides the decoded coefficient data into first components and second components. An eliminating unit eliminates the blank region specified by the specifying unit from the first components divided from the decoded coefficient data by the dividing unit. A synthesizing unit combines the first components from which the blank region has been eliminated by the eliminating unit with the second components divided from the decoded coefficient data by the dividing unit.01-19-2012
20100172569CELL IMAGE ANALYSIS APPARATUS, CELL IMAGE ANALYSIS METHOD, AND PROGRAM - A cell image analysis apparatus includes a threshold value storage unit storing a cell nucleus threshold value, a fragmented cell nucleus threshold value, and a cell nucleus area threshold value in advance, an image input unit inputting a cell image captured from a cell stained with a fluorescent substance, a cell nucleus region extraction unit extracting, from the input cell image, a region having an area equal to or larger than the cell nucleus area threshold value from among regions having a luminance value equal to or larger than the cell nucleus threshold value as a cell nucleus region, and a fragmented cell nucleus region extraction unit extracting, from the cell nucleus region, a region having a luminance value equal to or larger than the fragmented cell nucleus threshold value as a fragmented cell nucleus region.07-08-2010
20100172568Signet Ring Cell Detector and Related Methods - A detector and method for automatically detecting signet ring cells in an image of a biopsy tissue sample, includes finding in the image, points about which cell membranes appear in radial symmetry; selecting as candidate points, at least ones of the points that have an adjacent nuclei with a predetermined shape feature; and applying a convolutional neural network to the candidate points to determine which of the candidate points are signet ring cells.07-08-2010
20100290692SYSTEMS AND METHODS FOR AUTOMATED CHARACTERIZATION OF GENETIC HETEROGENEITY IN TISSUE SAMPLES - Systems and methods for the quantitative automated analysis of pathology samples identify groups of spatially-associated similar cells. Cells may be identified as belonging to a group on the basis of spatial location and biomarkers. In some embodiments the biomarkers are multicolour fluorescence in situ hybridization (FISH) signals. Characteristics of the cells in a group may be combined to provide quantitative FISH results that may compensate for variations and artefacts such as thin sectioning that can result in the loss of information due to damage. In heterogeneous tissue samples, grouping cells can permit quantitative results regarding pathological cells to be extracted despite the presences of infiltrating non pathological cells.11-18-2010
20100246927DARKFIELD IMAGING SYSTEM AND METHODS FOR AUTOMATED SCREENING OF CELLS - The disclosure is directed at a system and method for darkfield imaging system and method for automated cell screening of cells. The system and method acquires multi- or hyperspectral digital darkfield images of cells and then processes the images to obtain measurements which can then be supplied or displayed to a user to analyze.09-30-2010
20100246926SYSTEM AND METHOD FOR DISTINGUISHING BETWEEN BIOLOGICAL MATERIALS - The invention provides a method for distinguishing biological materials. The method provides: providing at least one segmented image of at least two cells; applying a distance transform to the at least one segmented image of the confluent cells; applying a region growing technique to the distance transform of the at least one segmented image to form a region grown image, wherein a plurality of regions are formed in the at least one segmented image; assigning at least one label to at least one of the plurality of regions of the at least one segmented image of the confluent cells; applying a merging technique to at least two of the plurality of regions if it is determined that at least two of the plurality of regions are neighboring regions; determining whether to assign a same label to the neighboring regions or retain existing labels; and merging the neighboring regions of the region grown image if labels are changed to form at least one image of at least one cell.09-30-2010
20120314930Image Classifier Training - Methods are disclosed that include: (a) applying a first stain to a first sample having a plurality of regions, where the first stain selectively binds to only a first subset of the regions of the first sample; (b) applying a second stain to the first sample, where the second stain binds to a second set of regions of the first sample; (c) obtaining an image of the first sample, and analyzing the image to obtain a first component image corresponding substantially only to spectral contributions from the first stain, and a second component image corresponding substantially only to spectral contributions from the second stain; and (d) training a classifier to identify regions of a second sample based on information derived from the first and second component images, the identified regions corresponding to the first subset of regions of the first sample.12-13-2012
20100208974Methods for Ranking Cellular Images - The methods described in this invention are used to analyze images of circulating tumor cells (CTC). Images are acquired from a number of platforms, including multiparameter flow cytometry, the CellSporter fluorescent microscopy imaging system and CellTracks Analyzer. These images are then ranked based on various properties and are presented to the user in order of most likely to least likely positive CTC events. The ranking method is useful to diagnose, monitor, and screen disease based on circulating rare cells, such as malignancy as determined by CTC.08-19-2010
20100080440BLOOD CELL IMAGE DISPLAY APPARATUS, SPECIMEN ANALYZING SYSTEM, BLOOD CELL IMAGE DISPLAY METHOD AND COMPUTER PROGRAM PRODUCT - A blood cell image display apparatus comprising: a classifier for classifying blood cell images, which are obtained by imaging a blood smear prepared from a blood specimen, in accordance with types of blood cells in the blood cell images; an information receiver for receiving information relating to the blood specimen; a display section; and a display controller for determining a type of blood cell as a display object on the basis of the information relating to the blood specimen, and displaying a blood cell image classified as the determined type on the display section, is disclosed. A specimen analyzing system, a blood cell image display method, and a computer program product are also disclosed.04-01-2010
20110182494Method Of Identification Of Cancerous And Normal Cells - A method is described for distinguishing between cancerous and normal human cells. The method includes collecting cells; preparing cells for scanning; scanning of the prepared cells by means of atomic force microscopy; processing of the obtained images through specific algorithms; wherein the algorithms allowing one to identify whether the cell is cancerous or normal.07-28-2011
20120257811COMPUTERIZED METHODS FOR TISSUE ANALYSIS USING N-GENE PROFILING - A computerized method for immunohistochemistry analysis of tissue utilizes digital images of multiple adjacent tissue sections aligned within a computerized software and processed with an algorithm to quantify a two-dimensional IHC signature score for each respective slide image. In various embodiments, the IHC score is performed over several adjacent sections and further processed to produce a three-dimensional IHC quantification referred to as an IHC signature map.10-11-2012
20120134570HIGH THROUGHPUT, OPTICAL METHOD AND SYSTEM FOR DETERMINING THE EFFECT OF A TEST SUBSTANCE ON NON-CONTIGUOUS LIVING CELLS - A rapid and efficient method and apparatus for detecting electrophysiologic, proarrhythmic, contractile, and other effects of substances such as compounds and drugs in native cellular cardiac preparations, the preparations representing an integrated cell-based pharmacologic response is disclosed. More specifically, a method to (1) rapidly and efficiently detect and verify the effects of chemicals, compounds and drugs on cardiac repolarization, contractility, and excitability using optically based techniques and customized simulation protocols, and (2) rapidly and efficiently screen and select compounds for electrophysiologic and proarrhythmic effects on cardiac myocytes is disclosed.05-31-2012
20120134569METHOD AND DEVICE FOR REDUCING POSITION-RELATED GRAY VALUE VARIATIONS BY MEANS OF A REGISTRATION OF IMAGE DATA SETS - The present invention relates to a device and to a method for reducing in particular position-related gray value variations of image data sets of an object (05-31-2012
20120170828AUTOMATED DETECTION OF MELANOMA - Embodiments herein exploit the optical sectioning capability of reflectance confocal microscopy to non-invasively survey the dermal-epidermal junction (DEJ), noting the irregularities associated with malignancy. Methods are provided to aid a clinician in diagnosing melanoma through pattern recognition to extract pertinent diagnostic information from large 3D confocal images. Identifying the combination of pagetoid melanocytes and DEJ breakdown increases the accuracy of detection. A method may be used to process a 3D confocal volume of images taken by a clinician of a suspicious lesion and deduce the depth location z of the first reflective surface (FRS) at each x-y position. This FRS is where the most superficial melanin resides. In this manner, the stratum corneum and epidermis are digitally stripped and no longer distract the clinician from the more diagnostically relevant pigmented cell network. The FRS is putatively either the DEJ for benign nevi or the depth of a pagetoid melanocyte at x,y above the DEJ. Thus by creating a 3D surface plot of the FRS for each lateral x,y point, the presence of pagetoid cells is identified by a discreet jump in the FRS above the level of the DEJ. The DEJ map may also be used to determine if breakdown of the DEJ is occurring.07-05-2012
20110188728METHODS OF GENERATING TROPHECTODERM AND NEURECTODERM FROM HUMAN EMBRYONIC STEM CELLS - Methods for inducing differentiation of stem cells into trophectoderm, neurectoderm, or progeny cells thereof are described, and mathematical and statistical image analysis methods and systems, for example, to identify differentiated cells and screen for agents which modulate differentiation are provided for separate or combined use.08-04-2011
20100054574METHOD FOR QUANTITATIVE VIDEO-MICROSCOPY AND ASSOCIATED SYSTEM AND COMPUTER SOFTWARE PROGRAM PRODUCT - A method of determining an amount of at least one molecular species in a sample from an image of the sample captured by an image acquisition device is provided, each molecular species being indicated by a dye. A dye space representation of a plurality of dyes is formed by orthogonally adding the correspondence tables of the dyes, each correspondence table having a plurality of normalized RGB triplets and incrementally extending from 0% to 100% transmittance. The dye space representation has one dimension for each dye and provides a reference model for a combination of the plurality of dyes. Each pixel of an image of the sample stained with the combination of the plurality of dyes is compared to the reference model, each pixel having a color defined by an RGB triplet, so as to determine an optimal combination of normalized RGB triplets from the respective correspondence tables of the dyes producing the color of the respective pixel. An artificial image of the sample is then formed from the normalized RGB triplets for each dye as determined from the optimal combination. The artificial image thereby indicates a distribution of the respective dye over the sample image and facilitates determination of the amount of the corresponding molecular species. Associated methods, systems, and computer software program products are also provided.03-04-2010
20120076391DIGITAL MICROSCOPE SLIDE SCANNING SYSTEM AND METHODS - Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.03-29-2012
20120076390METHODS FOR FEATURE ANALYSIS ON CONSECUTIVE TISSUE SECTIONS - Feature analysis on consecutive tissue sections (FACTS) includes obtaining a plurality of consecutive tissue sections from a tissue sample, staining the sections with at least one biomarker, obtaining a digital image thereof, and identifying one or more regions of interest within a middle of the consecutive tissue sections. The digital images of the consecutive tissue sections are registered for alignment and the one or more regions of interest are transferred from the image of the middle section to the images of the adjacent sections. Each image of the consecutive tissue sections is then analyzed and scored as appropriate. Using FACTS methods, pathologist time for annotation is reduced to a single slide. Optionally, multiple middle sections may be annotated for regions of interest and transferred accordingly.03-29-2012
20080212865Image Processing Method for a Microscope System - An embodiment is disclosed for performing the image processing for analyzing the results of a fluorescence in situ hybridization (FISH) microscopic automated sample analysis to determine specific chromosomal characteristics.09-04-2008
20120177279Automated Tuberculosis Screening - A method, system, and computer-readable medium for determining a number of mycobacteria in source image data of a sputum-smear sample are provided. First image data is extracted from the source image data of the sputum-smear sample. Binary image data is created from the extracted first image data. A plurality of groups of blobs is identified in the created binary image data. A distance between a pair of the identified plurality of groups of blobs is determined. If the distance is below a threshold value, the pair is combined as a single group of blobs. The determining is repeated for pairs of the identified plurality of groups of blobs, and the remaining groups of blobs are then classified.07-12-2012
20120177280METHODS AND APPARATUS FOR DIAGNOSIS AND/OR PROGNOSIS OF CANCER - The subject invention concerns methods for the detection, diagnosis, and/or prognosis of cancer by analyzing centrosomal features. In one embodiment, a method includes receiving an image of one or more cells; selecting a region of interest in one cell; segmenting the region of interest to delineate at least one centrosomal; extracting one or more features from the segmented image; and analyzing the extracted features to diagnose cancer. In another embodiment, the progression of cancer can be predicted through analysis and classification of the extracted features. In one embodiment, the method can be performed by a quantitative cancer analysis system including a diagnosis module and/or a prognosis module. In one embodiment, the method can be performed using an image processing system.07-12-2012
20090324050CELL IMAGE PROCESSOR AND CELL IMAGE PROCESSING METHOD - Cells showing specific characters can be separated from noise, extracted, and analyzed, through processing of a cell image. It is intended to provide a cell image processor (12-31-2009
20120082366PATHOLOGIC IMAGE DIAGNOSTIC SYSTEM, PATHOLOGIC IMAGE DIAGNOSTIC METHOD, AND PATHOLOGIC IMAGE DIAGNOSTIC PROGRAM - Pathologic tissue images are to be output which allow effective image diagnosis to be performed. There are included an index amount calculating unit that divides a captured pathologic image into a predetermined divisional areas and sets a measurement index value related to a reference of pathologic diagnosis for each divisional area; and a significance calculating module that sets, for each divisional area, a significance related to the pathologic measurement on the basis of both the measurement index value of each divisional area and the measurement index value of a respective divisional area adjacent to that divisional area. The significance calculating module extracts that one of the divisional areas in which the areas for which images of the living tissues have been captured represent a given percentage, and further the significance calculating module associates, with the extracted divisional area, the measurement index value and significance set therefor for transmission.04-05-2012
20120082365PATHOLOGIC TISSUE IMAGE ANALYZING APPARATUS, PATHOLOGIC TISSUE IMAGE ANALYZING METHOD, AND PATHOLOGIC TISSUE IMAGE ANALYZING PROGRAM - This invention is directed to improvement of the accuracy of cancer differentiation in the pathologic diagnosis using a pathologic tissue image analysis. There are included a stroma area/duct area detecting module for detecting, from a biological tissue image, a duct area on the basis of duct characteristic information indicating the characteristic of ducts included in a tissue area in the biological tissue image; an intraduct cell-nucleus detecting module for extracting, based on a predetermined pigment reference, cell nucleuses included in the detected duct area; and a duct formation evaluating module for calculating a heterotypic degree of each duct on the basis of the distribution state of the cell nucleuses for a predetermined base areas in each duct.04-05-2012
20120082364IMAGE PROCESSING SYSTEM - Provided is an image processing system including a multiband signal acquisition unit and a dye spectral property determining unit. The multiband signal acquisition unit acquires a multiband signal value of a subject specimen that has been stained with H&E dyes. The dye spectral property determining unit generates new spectral properties of the H dye and the E dye by varying standard spectral properties previously set to each of the H dye and the E dye according to a spectral property change model, and determines the spectral properties of the H dye and the E dye based on the generated new spectral properties and multiband signal values.04-05-2012
20120082362Method for analyzing biological specimens by spectral imaging - A method for analyzing biological specimens by spectral imaging to provide a medical diagnosis includes obtaining spectral and visual images of biological specimens and registering the images to detect cell abnormalities, pre-cancerous cells, and cancerous cells. This method eliminates the bias and unreliability of diagnoses that is inherent in standard histopathological and other spectral methods. In addition, a method for correcting confounding spectral contributions that are frequently observed in microscopically acquired infrared spectra of cells and tissue includes performing a phase correction on the spectral data. This phase correction method may be used to correct various types of absorption spectra that are contaminated by reflective components.04-05-2012
20120082361METHOD - In one aspect the present invention provides a method for selecting a cell or cell colony which produces a polypeptide of interest, comprising a) providing a medium comprising cells and a detection agent, wherein the detection agent is associated with a detectable signal and the detection agent is capable of binding to the polypeptide of interest; b) providing a solid phase having a capture agent disposed thereon, wherein the capture agent is capable of binding to the polypeptide of interest; c) contacting the medium with the solid phase; d) detecting the signal associated with the detection agent; and e) selecting a cell or cell colony associated with the signal, wherein presence of the signal is indicative of a cell or cell colony which produces the polypeptide of interest.04-05-2012
20100254589METHOD AND SYSTEM FOR IMAGE ANALYSIS - The present invention relates generally to a method for determining the level of expression of one or more candidate objects of interest in a biological sample. In particular, the present invention relates to a method for determining the level of expression of one or more candidate objects of interest using image analysis. More specifically, the present invention relates to a method and a system for determining the level of expression of one or more candidate objects of interest using an automated (computer-aided) image analysis system.10-07-2010
20100254588AUTOMATED BONE MARROW CELLULARITY DETERMINATION - The invention determines cell to fat ratio statistic, applicable in the field of pathology, in a greatly improved manner over manual or prior art scoring techniques. The cellular areas are identified and displayed in an easy to read format on the computer monitor, printer output or other display means, with average cellularity, nuclear quantity distribution at a glance. These output data are an objective transformation of the subjective visible image that the pathologist or scientist relies upon for diagnosis, prognosis, or monitoring therapeutic perturbations. The invention uses multi-stage thresholding and segmentation algorithms in RGB and HSB spaces, auto-thresholding on red and blue channels in RGB to get the raw working image of all cells, then refines the working image with thresholding on hue and intensity channels in HSB using an adaptive parameter epsilon in entropy mode, and further separates different groups of cells within the same class, by auto-thresholding within the working image region.10-07-2010
20080298667IMAGE PORTION IDENTIFICATION METHODS, IMAGE PARSING METHODS, IMAGE PARSING SYSTEMS, AND ARTICLES OF MANUFACTURE - Image portion identification methods, image parsing methods, image parsing systems, and articles of manufacture are described. According to one embodiment, an image portion identification method includes accessing data regarding an image depicting a plurality of biological substrates corresponding to at least one biological sample and indicating presence of at least one biological indicator within the biological sample and, using processing circuitry, automatically identifying a portion of the image depicting one of the biological substrates but not others of the biological substrates.12-04-2008
20120237106Automatic image analysis and quantification for fluorescence in situ hybridization - An analysis system automatically analyzes and counts fluorescence signals present in biopsy tissue marked using Fluorescence in situ Hybridization (FISH). The user of the system specifies classes of a class network and process steps of a process hierarchy. Then pixel values in image slices of biopsy tissue are acquired in three dimensions. A computer-implemented network structure is generated by linking pixel values to objects of a data network according to the class network and process hierarchy. Objects associated with pixel values at different depths of the biopsy tissue are used to determine the number, volume and distance between cell components. In one application, fluorescence signals that mark Her2/neural genes and centromeres of chromosome seventeen are counted to diagnose breast cancer. Her2/neural genes that overlap one another or that are covered by centromeres can be accurately counted. Signal artifacts that do not mark genes can be identified by their excessive volume.09-20-2012
20120237107SYSTEM AND METHODS FOR DIGITAL EVALUATION OF CELLBLOCK PREPARATIONS - Digital evaluation of cellblock preparations to determine the type and extent of disease in order to identify the best approach for treatment without the need for additional testing or sampling.09-20-2012
20110002525Method for distinguishing living cells during cell observation, image processing program for cell observation, and image processing device - An image processing program for cell observation in which living cells can be distinguished from foreign matter other than living cells during cell observation under wide observation conditions. The image processing program includes a step for capturing first and second images of an object positioned in the observation field of view at a predetermined time interval, a step for extracting a representation of the object shown in the obtained images, steps for aligning the rotational angular orientation of the object in the image plane and computing a correlation value, a step for determining whether the object is a living cell on the basis of the computed correlation value, and steps for outputting the result of the decision made with regard to the object.01-06-2011
20120093387METHOD FOR AUTOMATED PROCESSING OF DIGITAL IMAGES OF TISSUE MICRO-ARRAYS (TMA) - A method and system for automated quantitation of tissue micro-array image (TMA) digital analysis. The method and system automatically analyze a digital image of a TMA with plural TMA cores created using a needle to biopsy or other techniques to create standard histologic sections and placing the resulting needle cores into TMA. The automated analysis allows a medical conclusion such as a medical diagnosis or medical prognosis (e.g., for a human cancer) to be automatically determined. The method and system provides reliable automatic TMA core gridding and automated TMA core boundary detection including detection of overlapping or touching TMA cores on a grid.04-19-2012
20100189338SYSTEMS AND METHODS FOR COUNTING CELLS AND BIOMOLECULES - The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.07-29-2010
20110235891VESSEL ANALYSIS - A system for performing vessel analysis uses display means (09-29-2011
20120321169Shape Based Conditional Random Fields for Segmenting Intracranial Aneurysms - A method for segmenting intracranial aneurysms in digital medical images includes extracting a mesh representing a vessel surface from a volumetric digital image, the mesh comprising a set of points {p12-20-2012
20110158500Method and apparatus relating to sample card punching - The invention relates to method and apparatus for determining from a sample carrier containing an impregnated biological sample a sample region to be removed. The method comprises optically imaging the sample carrier into a digital matrix form, where the optical brightness of each physical location of the sample is represented by elements of the matrix; determining a first threshold value at least partly on the basis of the elements of the matrix; and calculating coordinates corresponding to the sample region to be removed from the sample carrier, the coordinates being such that the sample region contains only areas of the sample carrier having an optical brightness lower than or equal to said first threshold value. The invention allows for more efficient use of samples in sample cards.06-30-2011
20110158499INFORMATION MANAGEMENT IN AUTOMATED PROCESSING OF BIOLOGICAL GROWTH MEDIA - This disclosure is directed to information management techniques that may be used during automated processing of biological growth media. In one embodiment, a method comprises reading one or more identification elements of a biological growth medium, identifying manufacturing information related to the biological growth medium based on the one or more identification elements of the biological growth medium, identifying sample information related to a sample being tested by the biological growth medium based on the one or more identification elements of the biological growth medium, generating one or more images of the biological growth medium, counting biological agents formed on the biological growth medium based on the one or more images, wherein counting the biological agents comprises generating a count value, and associating the manufacturing information, the sample information and the count value with the one or more identification elements of the biological growth medium.06-30-2011
20120328178Method for analyzing biological specimens by spectral imaging - A method for registering a visual image and a spectral image of a biological sample includes aligning a first set of coordinate positions of a plurality of reticles on a slide holder and a second set of coordinate positions of the plurality of reticles on the slide holder. The method further includes generating a registered image of a visual image of a biological sample and a spectral image of the biological sample based upon the alignment of the first and second set of coordinate positions.12-27-2012
20120328177Method for Imaging and Differential Analysis of Cells - Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.12-27-2012
20120134571CELL CLASSIFICATION METHOD, IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING THE METHOD, AND METHOD FOR PRODUCING CELL AGGREGATION - An image processing program AP comprises: a step (S05-31-2012
20100202679AUTOMATIC CELL ANALYZER AND AUTOMATIC CELL ANALYZING METHOD - A sharp image showing a thin projecting part is acquired without using a complex dyeing method, and the analysis accuracy is improved. An automatic cell analyzer (08-12-2010
20130016891User interface method and system for management and control of automated image processing in high content screening or high throughput screening - A user interface method and system for controlling automated image processing operations of HCS and/or HTS systems includes a graphical interface to enable user designation of an image naming convention, image sources and destinations, image processing channels, processing parameter values, and processing spatial designations.01-17-2013
20130016892FEATURE-BASED REGISTRATION OF SECTIONAL IMAGES - The present invention relates to a method and a system for obtaining and analysing image pairs, obtained as sections of specimen. The invention facilitates registration of two corresponding images, one from each section of the specimen. The invention includes performing a registration process of the two images thereby obtaining a mathematical transformation rule and afterwards using said transformation rule for each image field identified in one image allowing that the corresponding image field in the other image may be identified as well. After the corresponding image pairs have been obtained using the method of the present invention, the sections can be assessed, such as by identifying the counting events for at least one type of object on the image fields within at least one corresponding image pair, optionally using automatic means.01-17-2013
20110150314SPECTRAL ANALYSIS OF BIOLOGICAL GROWTH MEDIA - This disclosure is directed to imaging techniques and image analysis techniques for automated analysis of biological growth media. According to this disclosure, the spectral responses of biological growth media can be used to identify and count biological agents from images of biological growth media. The biological growth media may be illuminated with two or more different wavelengths of electromagnetic radiation, and images of the biological growth media can be captured under these different illuminations. The spectral reflectance values in one or more first images can be normalized based on the spectral reflectance values in one or more second images, wherein the first images are associated with a different wavelength of illumination than the second images. The normalization may allow for better identification of biological agents that manifest on the biological growth media.06-23-2011
20110158501METHOD FOR SAMPLE CELL ANALYSIS USING A VIRTUAL ANALYSIS PLATE - A method includes carrying out at least a first processing of a sample (06-30-2011
20120243768METHOD OF ANALYZING CELL STRUCTURES AND THEIR COMPONENTS - A cell is provided that contains a plurality of virus particles. A first image of a first virus particle and a second image of a second virus particle are taken by electron microscopy technology. The first virus particle is characterized as being in a first maturity stage and the second virus particle as being in a second maturity stage. The first image and the second image are transformed to first and second gray scale profiles, respectively, based on pixel data. The first and second gray scale profiles are then saved as first and second templates, respectively. A third virus particle in a third image is identified. The third image is transformed into a third gray scale profile. The third gray scale is compared to the first and second template to determine a maturity stage of the third virus particle.09-27-2012
20120243767METHOD FOR TRACKING CELLS - Simple, high-precision cell tracking is realized. Provided is a method for tracking cells, comprising an image acquisition step (S09-27-2012
20080253642Method of Processing an Image - A method of processing an input image produces an output image emphasising peak-like structures. The input image comprises input pixels and each input pixel has an intensity characteristic. The output image comprises corresponding output pixels and each output pixel has a value. The method comprises the step of calculating for an input pixel the second derivative of the intensity characteristic in two orthogonal directions, one of the directions being the direction of maximum curvature. The product of the two second derivatives is calculated and the value of the output pixel corresponding to the input pixel is determined in dependence on the product.10-16-2008
20080247628Determination of a Change in a Cell Population - The present invention relates to a method and a system for determination of a change in a cell population, as well as a method for using said method and system for estimating a quality measure of embryos and for selecting embryos for in vitro fertilisation, said method comprising the steps of sequentially acquiring at least two images of the cell population, comparing at least a part of the at least two images obtaining at least one difference image, computing a parameter from the at least one difference image, based on said computed parameter determining whether a change has occurred.10-09-2008
20080226151Device and software for screening the skin - The present invention provides devices for screening the skin of an individual in real time using a region-fusion based segmentation with narrow band graph partitioning algorithm to analyze and classify a region of interest of the skin as benign or malignant. Also, provided is a method for screening the skin of an individual using the devices described herein. In addition the present invention provides a digital processor-implemented system for classifying a region of interest on the skin and a processor readable medium having processor-executable instructions to perform skin cancer detection.09-18-2008
20130177231SPECTRAL ANALYSIS OF BIOLOGICAL GROWTH MEDIA - This disclosure is directed to imaging techniques and image analysis techniques for automated analysis of biological growth media. According to this disclosure, the spectral responses of biological growth media can be used to identify and count biological agents from images of biological growth media. The biological growth media may be illuminated with two or more different wavelengths of electromagnetic radiation, and images of the biological growth media can be captured under these different illuminations. The spectral reflectance values in one or more first images can be normalized based on the spectral reflectance values in one or more second images, wherein the first images are associated with a different wavelength of illumination than the second images. The normalization may allow for better identification of biological agents that manifest on the biological growth media.07-11-2013
20110262025Cosmetic Compositions and Methods for Maintaining and Improving Barrier Function of the Stratum Corneum and to Reduce the Visible Signs of Aging in Skin - Gene panels, biomarker panels and microarrays relating to lipid formation in stratum corneum of human skin as a function of extrinsic and/or intrinsic aging conditions, and methods for assessing the age status of human skin, methods for identifying or evaluating an agent as effective for improving stratum corneum barrier maintenance and/or repair properties in aged skin, and cosmetic composition comprising the identified agents are all provided.10-27-2011
20130142413SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY - In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.06-06-2013
20130170728PROCESS AND SYSTEM FOR ANALYZING THE EXPRESSION OF BIOMARKERS IN A CELL - The invention relates generally to a process of analyzing and visualizing the expression of biomarkers in an individual cell wherein the cell is examined to develop patterns of expression by using a grouping algorithm, and a system to perform and display the analysis.07-04-2013
20110274340IMAGE ANALYSIS APPARATUS, IMAGE ANALYSIS METHOD, IMAGE ANALYSIS PROGRAM AND RECORDING MEDIUM - A cancer determination apparatus (11-10-2011
20110274339Method for kinetic characterization from temporal image sequence - A computerized derivable kinetic characterization measurement method for live cell kinetic characterization inputs kinetic recognition data for a plurality of time frames. A single cell measurement step is performed using the kinetic recognition data for a plurality of time frames to generate single cell feature for a plurality of time frames output. The single cell feature includes cell morphological profiling feature. A kinetic measurement step uses the single cell feature for a plurality of time frames to generate kinetic feature output. A trajectory measurement step uses the single cell feature for a plurality of time frames and the kinetic feature to generate trajectory feature output. An interval measurement step uses the kinetic feature to generate interval feature output. A cell state classifier step uses the interval feature to generate cell state output. A state based measurement uses the single cell feature, the kinetic feature and the cell state to generate state based feature output.11-10-2011
20110274338Image analysis for cervical neoplasia detection and diagnosis - The present invention is an automated image analysis framework for cervical cancerous lesion detection. The present invention uses domain-specific diagnostic features in a probabilistic manner using conditional random fields. In addition, the present invention discloses a novel window-based performance assessment scheme for two-dimensional image analysis, which addresses the intrinsic problem of image misalignment. As a domain-specific anatomical feature, image regions corresponding to different tissue types are extracted from cervical images taken before and after the application of acetic acid during a clinical exam. The unique optical properties of each tissue type and the diagnostic relationships between neighboring regions are incorporated in the conditional random field model. The output provides information about both the tissue severity and the location of cancerous tissue in an image.11-10-2011
20110274337System, method, and kit for processing a magnified image of biological material to identify components of a biological object - A system, method and kit for processing an original image of biological material to identify certain components of a biological object by locating the biological object in the image, enhancing the image by sharpening components of interest in the object, and applying a contour-finding function to the enhanced image to create a contour mask. The contour mask may be processed to yield a segmented image divided by structural units of the biological material.11-10-2011
20110274336OPTIMIZING THE INITIALIZATION AND CONVERGENCE OF ACTIVE CONTOURS FOR SEGMENTATION OF CELL NUCLEI IN HISTOLOGICAL SECTIONS - A computer-implemented segmentation method is used to process an image representing a plurality of nuclei. The method is implemented in a computer having a processor and a physical memory. A set of instructions are provided to the processor the physical memory of the computer. The processor is configured by executing the set of instructions in the physical memory so as to automatically segment the image by: thresholding a grey-scale image to create a black and white image; identifying objects in the black and white image and removing objects failing to meet predetermined criteria; extracting objects; and applying an edge detector on the segmented image to identify the edges of the nuclei. Overlapping nuclei are split to improve results.11-10-2011
20080219542Systems for characterizing localization in biological cells - Systems for characterizing localization in biological cells.09-11-2008
20120250975IDENTIFICATION AND CLASSIFICATION OF VIRUS PARTICLES IN TEXTURED ELECTRON MICROGRAPHS - The method is for the identification and characterization of structures in electron micrographs. Structures in a first image are selected. The structures have a first shape type deformed in a first direction. The selected structures are transformed to a second shape type different from the first shape type. The transformed structures of the second shape type are used to form a plurality of templates. A new structure in a second image is identified. The new structure has the first shape type. The second shape type structure of each template is deformed in the first direction. It is determined which template is a preferred template that best matches the new structure.10-04-2012
20130094749MODEL-BASED CORONARY ARTERY CALCIUM SCORING - A system and method are provided for model-based coronary artery calcium (CAC) scoring. A model image of a heart region is aligned with an image of a patient's heart region in order to more easily identify the coronary arteries and other relevant anatomical features in the image. Once the images are aligned, relevant calcium plaques are identified by their presence within a coronary artery, and the relevant plaques are then labeled by the specific coronary artery in which they are located. The coronary arteries with the labeled plaques are scored individually based on their size and X-ray attenuation, and an overall score based on all of the relevant plaques is then computed, which is related to the patient's risk for coronary artery disease.04-18-2013
20130114875Methods and Apparatus for Image Analysis Using Profile Weighted Intensity Features - A new morphological feature referred to herein as profile weighted intensity feature is provided, useful for automated classification of objects, such as cells, that are depicted in digital images. In certain embodiments, the profile weighted intensity feature is determined by automatically identifying a border of a cell in an input image, determining a distance image for the cell, computing a profile function for the cell, and computing a mean intensity of at least a portion of the input image weighted by the profile function for the cell.05-09-2013
20130114874Methods and Apparatus for Image Analysis Using Threshold Compactness Features - A new family of morphological features, referred to herein as threshold compactness features, is provided, useful for automated classification of objects, such as cells, in images. In one embodiment, one or more thresholds and/or binary masks are applied to an image, and one or more provisional objects within a cell in the image are automatically identified. The threshold compactness of the cell is computed as a function of area S of the one or more provisional objects and border length P of the one or more provisional objects. Computation of threshold compactness allows cells in an image to be distinguished and characterized. Compared to previous techniques, the methods and apparatus described herein are more robust and computationally efficient.05-09-2013
20130101199IMAGE ANALYSIS - A first aspect of the invention relates to an apparatus 04-25-2013
20130129181COMPACT DARK FIELD LIGHT SOURCE AND DARK FIELD IMAGE ANALYSIS AT LOW MAGNIFICATION - The invention relates to image analysis of dark field images obtained at low magnification below 10:1. Image analysis of dark field images obtained at low magnification can be combined with analyses of images obtained in respect of the same section of a sample and same magnification but with other techniques such as fluorescent microscopy. The system and method can be used e.g. for particle counting, particle size measurement, particle size distribution, morphology measurement, where the particles can be cells and/or cell parts. The invention also relates to a compact dark field light source unit, a system or apparatus including a microscope which by itself is compact and comprises the mentioned dark field light source unit.05-23-2013
20080205739FLUID FOCUSING FOR POSITIONAL CONTROL OF A SPECIMEN FOR 3-D IMAGING - A method for loading a sample for imaging by an optical tomography system. A sample volume including at least one microscopic sample and a viscous fluid is coaxially loaded into a sample delivery tube. The sample volume is impelled through a focus cell into a capillary tube, where the capillary tube has a smaller crossectional area than the sample delivery tube, so that a reduced volume of the at least one microscopic sample and viscous fluid is constrained to a central region within the capillary tube.08-28-2008
20080199066Sample image obtaining method, sample image obtaining apparatus and sample image filing system - The present invention is to present a sample image obtaining method that is capable of reflecting the blood cell concentration of a sample and increasing the speed of the examination. The sample image obtaining method comprises steps of: (a) obtaining a wide area image of a sample by imaging, at a predetermined magnification, a wide area including a smearing end of a smear region on a slide glass where the sample is smeared; (b) detecting the smearing end based on luminance information of the wide area image of the sample; (c) determining an imaging region in the smear region based on the detected smearing end; and (d) obtaining a sample image by imaging the determined imaging region at a higher magnification than the predetermined magnification.08-21-2008
20080199065Method, System and Device for In-Vivo Biopsy - A system and method for obtaining a sample from, for example, endo-lumina areas. One or more devices (08-21-2008
20110229008SAMPLE IDENTIFICATION DEVICE AND SAMPLE IDENTIFICATION METHOD - A cell stethoscope 09-22-2011
20120275681DEVICE FOR HARVESTING BACTERIAL COLONY AND METHOD THEREFOR - When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.11-01-2012
20130156286SYSTEMS AND METHODS FOR COLLECTING AND TRANSMITTING ASSAY RESULTS - Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.06-20-2013
20130156287OBSERVATION DEVICE, OBSERVATION PROGRAM, AND OBSERVATION SYSTEM - An observation device (06-20-2013
20130182935APPARATUS AND METHOD FOR TRACKING CONTOUR OF MOVING OBJECT, AND APPARATUS AND METHOD FOR ANALYZING MYOCARDIAL MOTION - A moving object contour tracking apparatus includes a contour tracking section for performing, by taking an initial contour of the moving object in a predetermined image slice as a starting contour, contour tracking in a first time direction to acquire a first contour of the moving object and contour tracking in a second time direction to acquire a second contour of the moving object in each image slice; a contour comparison section for calculating, in the predetermined image slice, a similarity between the first contour and the initial contour and a similarity between the second contour and the initial contour; and a contour correction section for taking the contours in the image slices that are acquired in a contour tracking direction corresponding to the greater one of the two similarities as the contours of the moving object in the respective image slices.07-18-2013
20130182936INFORMATION PROCESSING DEVICE, INFORMATION PROCESSING SYSTEM, INFORMATION PROCESSING METHOD, PROGRAM, AND RECORDING MEDIUM - The present invention provides an information processing apparatus capable of accurately separating parenchymal cells and stromal cells from each other regardless of the staining intensity of the cells. The information processing apparatus is an information processing apparatus 07-18-2013
20110311123Method and System for Automated Detection of Immunohistochemical (IHC) Patterns - A method and system for automated detection of immunohistochemical (IHC) patterns. The method and system is able to automatically differentiate an epithelial cell part from a non-epithelial cell part of a digital image of a tissue sample to which an IHC compound has been applied. The method and system help to automatically correct errors made in a manual interpretation of a pathological or other manual analysis of tissue samples for diseases such as a human cancer.12-22-2011
20130188857INFORMATION PROCESSING APPARATUS, INFORMATION PROCESSING SYSTEM, INFORMATION PROCESSING METHOD, PROGRAM, AND RECORDING MEDIUM - The present invention provides an information processing apparatus capable of selecting a region of cancer cells in any tissue sample image and easily and accurately counting the number of the cancer cells. The information processing apparatus is an information processing apparatus 07-25-2013
20120020542SYSTEM FOR DETECTING INFECTIOUS AGENTS USING COMPUTER-CONTROLLED AUTOMATED IMAGE ANALYSIS - A method for providing quantitative information regarding the extent of infection of host cells by an infectious agent. A microscope image of a specimen of a bodily fluid is analyzed using image processing techniques to quantify the percentage of the area of the specimen that is infected.01-26-2012
20130195344ASSAY IMAGE ACQUISITION SYSTEM AND METHOD - A computer-implemented method of imaging an assay sample having a set of individual samples using a camera is provided. A set of image frames is received. The set of image frames corresponds to an image stream of the assay obtained by the camera. A set of measurement results is identified. Individual measurement results in the set of measurement results are respectively associated with individual image frames in the set of image frames. A subset of measurement results is selected based, at least in part, on an exposure time. Individual measurement results in the subset of measurement results are combined to obtain an integrated measurement result.08-01-2013
20130202183METHOD FOR IMAGING AND DIFFERENTIAL ANALYSIS OF CELLS - Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.08-08-2013
20130202182METHODS AND SYSTEMS FOR ESTIMATING GENETIC CHARACTERISTICS FROM BIOMETRIC MEASUREMENTS - Methods and devices are disclosed for collecting fingerprint and genetic information from an individual during a single collection session. A skin site is illuminated by direct imaging of the skin site using light reflected from the illuminated skin site. A cell of the individual, such as a skin cell, is retrieved from the collection surface.08-08-2013
20130202181INTEGRATION OF FILTERS AND PHASE UNWRAPPING ALGORITHMS FOR REMOVING NOISE IN IMAGE RECONSTRUCTION - A method to detect and remove noise in image reconstruction. The method includes integration of filters and phase unwrapping algorithms for removing speckle noise, residual noise and noise at the lateral surface of height discontinuities. The method is used for generating a noise-free unwrapped phase map and hence, a successful image reconstruction of an object image.08-08-2013

Patent applications in class Cell analysis, classification, or counting

Patent applications in all subclasses Cell analysis, classification, or counting