Class / Patent application number | Description | Number of patent applications / Date published |
204459000 | Isoelectric focusing (i.e., using pH variation) | 20 |
20080283400 | Separation Medium for Use in Chromatography - The invention relates to a separation medium for chromatography which is structured in that way that the flow rate is in one preselected direction larger than perpendicular to that direction. | 11-20-2008 |
20080289963 | Gel for Isoelectric Focusing - The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel. | 11-27-2008 |
20080314750 | Method for Pre-Fractionation of Complex Samples - The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps:
| 12-25-2008 |
20090229979 | FACILE METHOD AND APPARATUS FOR THE ANALYSIS OF BIOLOGICAL MACROMOLECULES IN TWO DIMENSIONS USING COMMON AND FAMILIAR ELECTROPHORESIS FORMATS - An ensemble of components and methods are disclosed for utilizing two-dimensional electrophoresis in polyacrylamide or related polymer gels using common, existing and familiar electrophoresis formats and equipment. The disclosed invention makes two-dimensional electrophoresis convenient and easy to use for individuals already using vertical “mini-gel” type systems. The invention discloses the combination of pre-cast disposable gels for both first and second separation dimensions using novel support devices and cassettes that simplify the difficult multiple sample handling and processing steps inherent in ordinary two-dimensional electrophoresis methods. Devices and methods are disclosed in said invention that provide exceptional gel to gel reproducibility. | 09-17-2009 |
20090314639 | MEANS AND DEVICES FOR ELECTRO-FILTRATION OF MOLECULES - The present invention relates to means and devices for electro-filtration of molecules. The membranes comprise N-acryloyl-tris(hydroxymethyl)aminomethane (NAT) covalently linked to a support. The invention further encompasses compositions comprising an isoelectric buffer covalently bound to N-acryloyl-tris(hydroxymethyl)aminomethane (NAT). In particular, the present invention relates to membranes and devices allowing an isoelectric filtration of molecules in solution. | 12-24-2009 |
20100065428 | ISOELECTRIC FOCUSING GELS AND METHODS OF USE THEREOF - Provided herein are rapidly-rehydratable prior-cast, dehydrated, electrophoresis separation media particularly useful for isoelectric focusing, including methods of making, and methods of use thereof. | 03-18-2010 |
20100181197 | ISOELECTRIC POINT MARKERS - The present invention relates to the field of isoelectric focussing or 2D electrophoresis, in particular isoelectric point markers with fluorescence detection. The marker comprises an oligopeptide covalently labelled with a fluorescent dye and is characterised in that the dye has a net charge that will maintain the overall net charge of the oligopeptide upon being bound to the oligopeptide. The invention also relates to an electrophoretic method for analysis of proteins using these markers. | 07-22-2010 |
20100213065 | METHOD AND DEVICES FOR FORMING A PLURALITY OF WELLS ON A GEL - The present invention relates to methods and devices for forming a plurality of wells on a gel containing an analyte. The invention further provides a system for eluting a liquid analyte reagent mixture from a gel. The invention is useful in the separation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for separating and eluting peptides from isoelectric focusing gels. | 08-26-2010 |
20100243452 | ISOELECTRIC FOCUSING TRAY AND ELECTRODE ASSEMBLY FOR ALTERNATE GEL STRIP ORIENTATIONS - The procedures involved in isoelectric focusing that entail the use of an electric current can be performed with the gel side of the IPG strip facing either up or down in a tray and electrode assembly in which the electrodes are mounted on an insert from which the inner ends of the electrodes extend into the troughs to reside either below the IPG strips or above them. Slots in one part (either the insert or the tray) mate with walls or partitions in the other part to secure the two parts together, and the heights of the electrodes within the troughs of the tray can be adjusted by the height of the insert in the tray or by a resilient mounting of the electrode to the insert. | 09-30-2010 |
20100252435 | Method and device for separation and depletion of certain proteins and particles using electrophoresis - The present invention provides a novel and advantageous method for separating analytes by free flow electrophoresis. The methods are particularly suitable for depleting major constituents such as albumin from samples of biological origin, optionally combined with a further separation of the remaining portion of the sample. The sample portions recovered from the method can be used advantageously in downstream applications such as 1D or 2D-PAGE, HPLC or mass spectrometric analysis. Also provided are buffer systems, kits comprising such buffer systems, and devices for carrying out the free flow electrophoretic separation methods of the present invention. | 10-07-2010 |
20120085646 | Electrophoresis Separation Methods - An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent. | 04-12-2012 |
20120138463 | FACILE METHOD AND APPARATUS FOR THE ANALYSIS OF BIOLOGICAL MACROMOLECULES IN TWO DIMENSIONS USING COMMON AND FAMILIAR ELECTROPHORESIS FORMATS - An ensemble of components and methods are disclosed for utilizing two-dimensional electrophoresis in polyacrylamide or related polymer gels using common, existing and familiar electrophoresis formats and equipment. The disclosed invention makes two-dimensional electrophoresis convenient and easy to use for individuals already using vertical “mini-gel” type systems. The invention discloses the combination of pre-cast disposable gels for both first and second separation dimensions using novel support devices and cassettes that simplify the difficult multiple sample handling and processing steps Inherent in ordinary two-dimensional electrophoresis methods. Devices and methods are disclosed in said invention that provide exceptional gel to gel reproducibility. | 06-07-2012 |
20120138464 | GEL FOR ISOELECTRIC FOCUSING - The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel. | 06-07-2012 |
20130140182 | PROTEIN FRACTIONATION BASED ON pI - Methods and devices for detecting and collection analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided. | 06-06-2013 |
20130168248 | ELECTROPHORESIS GEL ASSEMBLY - An electrophoresis gel assembly comprising a gel slab, at least two sample wells and at least one focusing buffer well arranged between two adjacent sample wells and wherein focusing barriers are arranged between the focusing buffer well and the sample wells. A high conductivity focusing buffer can be applied to the focusing buffer well to obtain a lateral focusing of the sample bands during electrophoresis. | 07-04-2013 |
20130319863 | METHODS, COMPOSITIONS, AND KITS FOR PROTEIN CRYSTALLIZATION - The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient. | 12-05-2013 |
20140360878 | AFFINITY METHODS AND COMPOSITIONS EMPLOYING ELECTRONIC CONTROL OF PH - Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided. | 12-11-2014 |
20150362460 | MOBILE MICRO-LAB FOR CHEMICAL ANALYSIS OF FLUIDS - A micro-lab includes one or more electrophoresis devices each optically coupled to respective spectrometers and electronic signal processing, analysis and control, with fluids transported via a system of valves, tubes and pumps. The spectrograms are captured by a respective digital cameras, and chemical characteristics including molecular mobility, particle (molecular) charge, molecular weight, particle (molecular) pH, particle (molecular) dielectric, particle (molecular) conductivity, Raman spectrum of each chemical species, IR spectrum of particle (molecular) is determined, and principal component analysis is performed to identify and quantify chemical constituents. | 12-17-2015 |
20160084798 | ANALYSIS METHOD - An analysis method includes the steps of performing two-dimensional electrophoresis of analysis objects in an isoelectric direction and in a molecular weight direction that is substantially perpendicular to the isoelectric direction, cutting a medium containing the analysis objects, which have been subjected to the two-dimensional electrophoresis, in a lattice pattern along cut planes in the isoelectric direction and along cut planes in the molecular weight direction, and comparing the analysis objects between the cut mediums. | 03-24-2016 |
20160178578 | PH GRADIENTS CONTROLLED BY ELECTROLYSIS, AND THEIR USE IN ISOELECTRIC FOCUSING | 06-23-2016 |