20130068310 | Method and Apparatus for a Microfluidic Device - An microfluidic device and methods for its use, where the microfluidic device comprises: (a) a porous membrane, (b) a gradient layer defining a plurality of gradient micro-channels, where the gradient layer is coupled to a top surface of the membrane, (c) a distributor layer defining a plurality of distributor micro-channels, where the distributor micro-channels are coupled to the plurality of gradient micro-channels, where the distributor layer defines at least one inlet opening and at least one outlet opening, each inlet opening and outlet opening are coupled to the plurality of distributor micro-channels, and (d) self-supporting means coupled to one or more of the porous membrane, the gradient layer and the distributor layer. | 03-21-2013 |