TWO CELLS CO., LTD. Patent applications |
Patent application number | Title | Published |
20120329087 | CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME - A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A). | 12-27-2012 |
20120214738 | THERAPEUTIC AGENT AND THERAPEUTIC METHOD FOR PERIODONTAL DISEASES AND PULPAL DISEASES - The objects of the present invention are: to provide a therapeutic agent and a therapeutic method for periodontal diseases and pulpal diseases, a transplant for periodontal tissue regeneration, and a method for regenerating the periodontal tissue. | 08-23-2012 |
20120165255 | THERAPEUTIC AGENT AND THERAPEUTIC METHOD FOR PERIODONTAL DISEASES AND PULPAL DISEASES - The objects of the present invention are: to provide a therapeutic agent and a therapeutic method for periodontal diseases and pulpal diseases, a transplant for periodontal tissue regeneration, and a method for regenerating the periodontal tissue.
| 06-28-2012 |
20110076770 | CULTURING METHOD OF MESENCHYMAL STEM CELL - To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 g/ml at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring. | 03-31-2011 |
20100279412 | CULTURE MEDIUM ADDITIVE FOR USE IN SERUM-FREE CULTURING OF ANIMAL CELL, KIT AND USE THEREOF - Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium. | 11-04-2010 |
20100158982 | SHEET FOR GUIDING REGENERATION OF MESENCHYMAL TISSUE AND PRODUCTION METHOD THEREOF - To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell. | 06-24-2010 |
20090305255 | Substrate Protein for M-Phase Kinase and use Thereof - An isolated protein of (a) a protein having an amino acid sequence of SEQ ID No. 2; (b) a protein having an amino acid sequence partially different from the amino acid sequence of SEQ ID No. 2 that is a substrate of M-period kinase and has nucleic acid-methylating activity suppressed by phosphorylation; (c) a protein having the amino acid sequence of SEQ ID No. 2 that is phosphorylated by M-period kinase; or (d) a protein having an amino acid sequence partially different from the amino acid sequence of SEQ ID No. 2 that is phosphorylated by M-period kinase. | 12-10-2009 |
20090274635 | Bactericide having selectivity to cariogenic bacterium, and a method for sterilization of cariogenic bacterium - The bacteriolytic effect of a bacterial cell wall lytic enzyme can be increased by the addition of a surfactant to the enzyme. As a result, the time required for lysis of cariogenic bacterium with the bacterial cell wall lytic enzyme can be shortened, and the practical utility of the bacterial cell wall lytic enzyme (such as automutanolysin) as a prophylactic or therapeutic agent for dental caries can be improved. | 11-05-2009 |
20090253636 | Novel Antifungal Agent - It is intended to provide a novel peptide which can exert higher antifungal effect also on a patient whose organ function is impaired with no side effect problems. The peptide comprises the following amino acid sequence: (1) SEQ ID NO:1: Lys-Arg-Leu-Phe-Arg-Arg-Trp-Gln-Trp-Arg-Tyr, (2) SEQ ID NO:2: Arg-Arg-Trp-Gln-Trp-Arg-Met-Lys-Lys-Tyr, (3) SEQ ID NO:3: Lys-Arg-Trp-Gln-TTp-Arg-Leu-Tyr, or (4) SEQ ID NO:4: Lys-Arg-Leu-Phe-Lys-Tyr, an antifungal agent comprising the peptide, a composition having a fungicidal and/or growth inhibitory action against fungus containing an effective amount of the peptide as an active ingredient, a medical apparatus/device which contains the peptide, whereby a fungicidal action is conferred, and a fungicidal and/or growth inhibiting method against fungus comprising administering the peptide and the like. | 10-08-2009 |
20090221076 | METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION - An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration. | 09-03-2009 |
20080311056 | Bactericide Against Streptococcus Mutans and Streptococcus Sobrinus - The present invention provides an enzyme with lytic activity against cariogenic bacteria and a means for treating and preventing tooth decay using the enzyme. The enzyme provided by the present invention is a lytic enzyme produced by | 12-18-2008 |
20080283763 | Method of Measuring Exposed Dose of Ionizing Radiation - A method of measuring an exposed dose of ionizing radiation, containing: (a) extracting proteins from a tissue or blood collected from a living body, and (b) determining the content of at least one of products of LyGDI protein degraded by caspase-1 and caspase-3 in the extracted proteins. | 11-20-2008 |
20080220521 | SHEET FOR GUIDING REGENERATION OF MESENCHYMAL TISSUE AND PRODUCTION METHOD THEREOF - To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell. | 09-11-2008 |