| ROCHE MOLECULAR SYSTEMS, INC. Patent applications |
| Patent application number | Title | Published |
|---|
| 20120115148 | Reagents and Methods for Detecting Neisseria Gonorrhoeae - This invention provides compositions and methods for detecting | 05-10-2012 |
| 20120100599 | Nucleic Acid Isolation Using Polidocanol and Derivatives - This invention relates to a composition comprising a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof. The invention is further related to uses of this composition and to a kit comprising the composition according to the invention. The invention is further related to a method for the detection of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof, optionally isolating the nucleic acid, optionally amplifying the nucleic acid, and detecting the nucleic acid. The invention is further related to a method for the purification of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof and isolating the nucleic acid thereby purifying the nucleic acid. | 04-26-2012 |
| 20120094389 | Single Nucleotide Polymorphisms That Predict HCV Treatment Outcomes - The present invention is based on the discovery of associations that exist between single nucleotide polymorphisms (SNPs) on chromosome 4 and virological outcomes in a diverse population of patients with hepatitis C virus (HCV) who received interferon-based treatment. | 04-19-2012 |
| 20120094388 | Cassette With Multiwell Plates - A cassette is described which can hold at least two multiwell plates. The cassette comprising the multiwell plates can be loaded into a stacker of an automated analytical apparatus. While the stacker holds down the cassette, a lift pushes the multiwell plates upwards to make them accessible to a handler which can engage and transport the multiwell plates to a plate holder in the working area of the analytical apparatus. | 04-19-2012 |
| 20120094284 | Prediction of Early Virological Response in HCV Treatment - The present invention is based on the discovery of associations that exist between single nucleotide polymorphisms (SNPs) on chromosome 19 and virological outcomes in a diverse population of patients with hepatitis C virus (HCV) who received interferon-based treatment. | 04-19-2012 |
| 20120088227 | Devices and Process for Separating Plasma From a Blood Sample - The present invention pertains to a device for separating plasma from a blood sample comprising a stacked structure which is provided with a first portion including a separating member having a first surface for applying or receiving the blood sample, wherein the separating member is adapted to permit the passage of plasma but to inhibit the passage of cells, and a second portion including an absorptive member for absorbing the plasma, which has a second surface in contact with the separating member for receiving the plasma, wherein the absorptive member is adapted to generate a capillary pressure so as to draw plasma from the separating member to the absorptive member. The first portion is fixed to the backing member in a manner to be removed without destroying the absorptive member. The absorptive member is fixed to the backing member in a manner to be removed without destroying the absorptive member. | 04-12-2012 |
| 20120070831 | Amplification of Distant Nucleic Acid Targets Using Engineered Primers - The invention is a method of amplifying nucleic acids by synthesizing an engineered amplicon containing the sequence of interest, but omitting intervening sequences present in the template molecule. The method utilizes an “amplicon bridge” incorporated into the amplification primers. The length and content of the desired amplicon can be chosen by the operator and can contain unique regions for probe binding. | 03-22-2012 |
| 20120070820 | Probes and Methods for Hepatitis C Virus Typing Using Multidimensional Probe Analysis - This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV types (for example, selected from types 1, 2, 3, 4, 5 or 6), or to one of at least five subtypes (for example, subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a). These methods integrate the hybridization data from a plurality of HCV typing probes in a multidimensional analysis to make an HCV type assignment for an HCV in an experimental sample. The invention also provides related compositions, including, for example, the HCV typing probes and HCV typing diagnostic kits. | 03-22-2012 |
| 20120067801 | Analyzer Assembly Platform - A method for mounting and combination of an analytical apparatus with a platform for mounting such an apparatus comprising at least two units, and a platform on which said apparatus is mounted is disclosed, said platform comprising at least one platform element, and multiple feet for placing the platform elements on a surface. The feet are individually adjustable in height. | 03-22-2012 |
| 20120064511 | Generic Buffer For Amplification - The present invention relates to a method of amplifying a first and a second target nucleic acid in separate reaction receptacles, wherein said reaction receptacles comprise a solution comprising amplification reagents and oligonucleotides specific for said first or said second target nucleic acid, wherein said solution is the same for amplifying said first target nucleic acid and said second target nucleic acid. | 03-15-2012 |
| 20120058900 | HARDWARE ARCHITECTURE OF ANALYZERS - An analytical apparatus and a method for isolating and analyzing at least one analyte are described, comprising at least one module for receiving and dispensing a sample to be analyzed, at least one module for isolating said analyte to be analyzed, at least one module for analyzing said analyte, wherein said modules (i) to (iii) are arranged along an axis. | 03-08-2012 |
| 20120058479 | WORKFLOW TIMING BETWEEN MODULES - The invention relates to a method of isolating and analyzing an analyte using an analytical apparatus which comprises modules of different types, wherein any one module of one type has a specific, pre-defined timing for carrying out its workflow. | 03-08-2012 |
| 20120045751 | Generic Sample Preparation - The present invention relates to the sample preparation of nucleic acids for diagnostic purposes. More precisely, the invention provides a process for simultaneously isolating at least a first and a second target nucleic acid from a plurality of different types of fluid samples and optionally amplifying said isolated nucleic acids in a simultaneous manner. | 02-23-2012 |
| 20120022246 | Magnetic Pigment - Magnetic particles with an outer glass surface being essentially poreless or having pores of a diameter of less then 10 nm as well as ferromagnetic particles with a glass surface are preferentially useful for the isolation of biological material from samples. They provide a quick and reliable purification. | 01-26-2012 |
| 20120015405 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 01-19-2012 |
| 20120009628 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 01-12-2012 |
| 20120009564 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
| 20120009563 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
| 20120009562 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
| 20120009177 | GENE EXPRESSION MARKERS FOR PREDICTING RESPONSE TO INTERLEUKIN-6 RECEPTOR-INHIBITING MONOCLONAL ANTIBODY DRUG TREATMENT - This invention provides methods, compositions, and kits relating to gene product biomarkers where gene expression levels are correlated with therapeutic response of rheumatoid arthritis patients to treatment with an IL-6 receptor antagonist, such as an IL6-R antibody. The methods, compositions, and kits of the invention can be used to identify rheumatoid arthritis patients who are likely, or not likely, to respond to IL-6 receptor antagonist treatments. | 01-12-2012 |
| 20110318786 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-29-2011 |
| 20110318785 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-29-2011 |
| 20110312513 | BIOMARKERS FOR PREDICTING SUSTAINED RESPONSE TO HCV TREATMENT - The present invention is based on the discovery that in patients infected with Genotype 1 of the Hepatitis C virus (HCV-1) or Genotype 4 HCV (HCV-4) that undergo Triple Therapy treatment, certain biomarkers can be predictive of a patient achieving sustained virologic response | 12-22-2011 |
| 20110312041 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-22-2011 |
| 20110312040 | Preferential Amplification of mRNA Over DNA Using Chemically Modified Primers - The present invention relates to a method, oligonucleotides, reaction mixtures and kits for the selective amplification of a messenger RNA target comprising an exon-exon junction, using an oligonucleotide that comprises at least one nucleotide modified at the exocyclic amino group. | 12-22-2011 |
| 20110312039 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-22-2011 |
| 20110312038 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-22-2011 |
| 20110312037 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 12-22-2011 |
| 20110311982 | Nucleic Acid Isolation Using Polidocanol and Derivatives - This invention relates to a composition comprising a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof. The invention is further related to uses of this composition and to a kit comprising the composition according to the invention. The invention is further related to a method for the detection of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof, optionally isolating the nucleic acid, optionally amplifying the nucleic acid, and detecting the nucleic acid. The invention is further related to a method for the purification of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof and isolating the nucleic acid thereby purifying the nucleic acid. | 12-22-2011 |
| 20110311968 | Allele-Specific Amplification of Nucleic Acids - The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, having an internally-placed selective nucleotide complementary to only one variant of the target sequence wherein the allele-specific oligonucleotide is extended by a nucleic acid polymerase predominantly or exclusively when hybridized to the variant of the target sequence for which it has said complementary selective nucleotide. | 12-22-2011 |
| 20110306118 | Consumable hardware coding system - An analytical system is disclosed which recognizes different types of consumables with an identical footprint based on a unique surface geometry of each consumable. The analytical system comprises stackers into which said consumables can be loaded either automatically or by the user. The stacker for one specific type of consumable recognizes said type of consumable based on its unique surface geometry. | 12-15-2011 |
| 20110306097 | Multiwell plate and lid - The present invention relates to a multiwell plate for amplification and a lid with a foil for sealing the multiwell plate, wherein two positions of said lid on said plate exist, one position for storage, one position for sealing of the foil to the plate. The invention also relates to a method for. | 12-15-2011 |
| 20110306053 | Amplification System with Spatial Separation - An automated nucleic acid analysis method and analytical system are described comprising separate modules, wherein the air flow of any one of said modules is controlled and wherein at least the air flow between the module for isolation and purification of the analyte and the module for analysis of the analyte are separated. | 12-15-2011 |
| 20110306052 | Form-Locking Gripping System - The invention relates to an automated method of isolating and analyzing an analyte using a form-locking gripping mechanism. An analytical system comprising consumables and a handler, wherein the handler and consumable interact with a form-locking gripping mechanism are also included. | 12-15-2011 |
| 20110306051 | Method for separating and detecting an analyte - A method for separating and analyzing an analyte is provided which comprises, in a first position, transferring a liquid sample to a multiwell plate with a pipette tip, replacing the tips in the tip rack in the same position, and re-using the pipette tips for aspirating and dispensing liquid. | 12-15-2011 |
| 20110300621 | Process head positioning - A method is disclosed for mechanically aligning a process device and a rack which comprises mechanical engagement of positioning elements on the process device and positioning elements on the consumable. | 12-08-2011 |
| 20110300620 | Combo-tip Rack - The invention relates to a three-part rack for holding re-usable pipette tips. The rack comprises an upper, a lower and an insert rack. The rack is optimized for holding first and second types of pipette tips, and comprises contamination protection. | 12-08-2011 |
| 20110294168 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects of a variety of polymerase and reverse transcriptase inhibitors. Therefore, the mutant polymerases are useful in a variety of disclosed methods in the presence of such inhibitors. | 12-01-2011 |
| 20110281747 | BIOMARKERS FOR PREDICTING RAPID RESPONSE TO HCV TREATMENT - The present invention is based on the discovery that in patients infected with Genotype 1 of the Hepatitis C virus (HCV-1) or Genotype 4 HCV (HCV-4) that undergo Triple Therapy treatment, certain biomarkers can be predictive of a patient achieving RVR2. | 11-17-2011 |
| 20110269134 | Reagents and Methods for Detecting Neisseria Gonorrhoeae - This invention provides compositions and methods for detecting | 11-03-2011 |
| 20110262462 | POLYMORPHISM MARKERS FOR PREDICTING RESPONSE TO INTERLEUKIN-6 RECEPTOR-INHIBITING MONOCLONAL ANTIBODY DRUG TREATMENT - The present invention provides single nucleotide polymorphisms (SNPs) associated with clinical responsiveness of rheumatoid arthritis patients to treatment with an interleukin-6 receptor antibody such as tocilizumab, and methods of using such SNPs for predicting clinical response to treatment with the antibody. | 10-27-2011 |
| 20110212991 | Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors - The present invention provides methods and reagents for the detection of a mutation in BRAF and methods of selecting, patients for treatment if a B-Raf kinase inhibitor, such as a selective B-Raf kinase inhibitor. | 09-01-2011 |
| 20110203997 | MAGNETIC SEPARATION SYSTEM COMPRISING FLEXIBLE MAGNETIC PINS - A magnetic separation plate for use in methods employing magnetic particles, said magnetic separation plate comprising a support plate and magnetic pins in a predetermined geometrical arrangement, said magnetic pins having a fastening portion, an intermediate portion and a separation portion and being fastened to said support plate at their fastening portion, wherein said magnetic pins are individually displaceable at their separation portion. The invention further provides a method for the separation of magnetic particles using the separation plate. | 08-25-2011 |
| 20110201002 | 2'-Terminator Nucleotide-Related Methods and Systems - The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media. | 08-18-2011 |
| 20110172408 | RECOVERY OF NUCLEIC ACIDS FROM MAGNETIC GLASS PARTICLES - The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method. | 07-14-2011 |
| 20110151550 | System For Multi Color Real Time PCR - The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm. | 06-23-2011 |
| 20110137020 | Method and Device for Purifying Nucleic Acids - The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore. | 06-09-2011 |
| 20110136120 | Methods of Identification of Methylation of CPG - The present invention relates to the materials and methods for the identification of methylated nucleotides in samples of genomic DNA. The present invention also relates to methods of diagnosis of specific conditions by identification of specific methylated nucleotides. | 06-09-2011 |
| 20110129830 | DETERMINATION OF KIR HAPLOTYPES ASSOCIATED WITH DISEASE - Disclosed is a method of determining KIR genotypes for one or more individuals in parallel, the method comprising: for each individual, amplifying the polymorphic exon sequences of the KIR genes, pooling the KIR amplicons, performing emulsion PCR followed by pyrosequencing in parallel to determine all the amplicon sequences present in the individual to determine which KIR alleles are present in the individual. | 06-02-2011 |
| 20110111503 | Method for excising biological samples on a solid support - The present invention provides a method for excising a biological sample on a solid support while minimizing risk of carry over contamination. Furthermore, analytical kits and systems as well as an excising device for excising a biological sample on a solid support are provided. | 05-12-2011 |
| 20110095864 | Laboratory Device, Laboratory Rack Assembly and Method for Coupling an RFID Chip - The invention relates to devices and methods for the identification of test tubes in a test tube rack using RFID technology. | 04-28-2011 |
| 20110054852 | DETERMINATION OF ELBOW VALUES FOR PCR FOR PARABOLIC SHAPED CURVES - Systems and methods for processing PCR curves, and for identifying the presence of a parabolic-shaped PCR curve. Use of a piecewise linear approximation of a PCR curve enables a more realistic elbow value to be determined in the case of parabolic shaped PCR curves. | 03-03-2011 |
| 20110039264 | Format of Probes to Detect Nucleic Acid Differences - The invention provides, inter alia, novel probes, methods, reaction mixtures, and kits for detecting the presence or absence of a target nucleic acid sequence. | 02-17-2011 |
| 20110008225 | Chip Based Diagnostic Device - A diagnostic device comprising a sample inlet and a processing chamber having a window portion and a flat carrier having front surface, a back surface and one or more side surfaces, said front surface facing said processing chamber, said front surface having an active surface containing a diagnostic reagent immobilized to said active front surface and an extended front surface, wherein said window portion further contains a rim portion facing said extended front surface sealing said chamber versus said carrier through said extended front surface. | 01-13-2011 |
| 20100304372 | Liver Cancer Methods and Compositions - The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned. | 12-02-2010 |
| 20100268472 | DETERMINATION OF SINGLE PEAK MELTING TEMPERATURE BY PCR ANALOGY AND DOUBLE SIGMOID EQUATION - Systems and methods for determining melting temperatures, Tm, for DNA from melt curve data. The systems and methods also allow for quantitative determination of gene amount based on peak height. A PCR analogy is used to perform quantization of an acquired melting curve dataset. The melting curve is transformed using a horizontal flip and a horizontal translation, and a double sigmoid equation is then fit to the data. Inverse translation and inverse horizontal flip transforms are applied to the equation to produce an equation based solution of the melt curve dataset. The equation based solution of the melt curve is then used to determine the first derivative (e.g., Tm value) and peak height. | 10-21-2010 |
| 20100233686 | Multiplex Quantitative Nucleic Acid Amplification and Melting Assay - The invention is a single-tube multiplex assay, capable of simultaneously amplifying, detecting and quantifying multiple nucleic acid targets, using multiple hybridization probes, labeled with the same fluorescent reporter label, but each having a distinct melting temperature. The assay can be further multiplexed with the use of multiple sets of hybridization probes, each set labeled with a separate fluorescent reporter label. | 09-16-2010 |
| 20100209304 | DISPOSABLE FOR ANALYZING A LIQUID SAMPLE BY NUCLEIC ACID AMPLIFICATION - The invention refers to a disposable sample holding and processing device ( | 08-19-2010 |
| 20100196884 | Nucleic Acid Preparation - The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method speeds up overall reaction time without affecting the limit of detection. | 08-05-2010 |
| 20100173353 | Lysis Reagent Formulation - Subject matter of the invention is a lysis reagent formulation for binding components of a sample in the form of a tablet comprising a multitude of magnetic particles which are held together with the aid of excipients, a chaotropic salt and an excipient and the use of this lysis reagent formulation in analytical tests. | 07-08-2010 |
| 20100173311 | NUCLEIC ACID AMPLIFICATION WITH ALLELE-SPECIFIC SUPPRESSION OF SEQUENCE VARIANTS - The present invention is an improved method of selective amplification of certain variants of the target sequence, enhanced by allele-specific suppression of amplification of one or more of the other variants of the target sequence. The improvement is accomplished by providing an oligonucleotide, capable of hybridizing to the desired variant of the target sequence with the lesser affinity than to the undesired variants of the target sequence and optionally, by providing chemically modified primers and hot-start conditions. | 07-08-2010 |
| 20100173294 | DIAGNOSTIC TEST FOR SUSCEPTIBILITY TO B-RAF KINASE INHIBITORS - The present invention provides methods and reagents for the detection of a mutation in BRAF and methods of selecting patients for treatment if a B-Raf kinase inhibitor, such as a selective B-Raf kinase inhibitor | 07-08-2010 |
| 20100163493 | Method and Device Employing Centrifugal Force - The present invention includes a container and a method of separating one or more components of interest bound to magnetic particles using centrifugal forces. | 07-01-2010 |
| 20100159452 | Method For Detecting a Target Nucleic Acid in a Sample - The invention relates to a method for detecting a target nucleic acid in a sample using fluorescent probe pairs which include fluorescent reporter and quencher molecules which may be used in hybridization assays and in nucleic acid amplification reactions, especially polymerase chain reactions (PCR). | 06-24-2010 |
| 20100153066 | System and Methods for Monitoring a Thermoelectric Heating and Cooling Device - The present invention pertains to methods for monitoring a thermoelectric heating and cooling device ( | 06-17-2010 |
| 20100143901 | Nuclease-Free Real-Time Detection of Nucleic Acids - The invention is a method for amplification and detection of nucleic acids using primers and at least one hybridization probe labeled with a first fluorescent moiety and a second moiety, capable of changing the fluorescence of said first fluorescent moiety. The method comprises the steps of effecting denaturation of said target, formation of hybrids between said primers and probe and said target and detecting the change in fluorescence of said first fluorescent moiety, upon formation of said hybrids. Reaction mixtures and kits for practicing the method of the present invention are also disclosed. | 06-10-2010 |
| 20100130372 | ALGORITHMS FOR CLASSIFICATION OF DISEASE SUBTYPES AND FOR PROGNOSIS WITH GENE EXPRESSION PROFILING - Methods for generating a normalized expression signal for microarray data based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays. The method typically includes receiving microarray data representing a plurality of probe pairs for a single microarray, determining, for each probe pair, differences between intensities of perfect match (PM) probes and intensities of mismatched (MM) probes, determining a difference signal, D, based on the determined differences, and scaling the difference signal, D, to produce an expression signal, DS. The method also typically includes normalizing the expression signal based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays. | 05-27-2010 |
| 20100124744 | DETECTION OF TARGET VARIANTS USING A FLUORESCENT LABEL AND A SOLUBLE QUENCHER - Methods, reaction mixtures and systems for detecting the presence or absence of a target nucleic acid variant from a selection of possible variants is described. | 05-20-2010 |
| 20100122807 | Cooler / Heater Arrangement - The present invention relates to a device for heating and cooling an object in a controlled manner permitting a good thermal contact between the thermal block, the element for heating and cooling and the heat sink without the need for using a thermal interface material, an instrument comprising such a device and a method for conducting a thermal profile using the device. | 05-20-2010 |
| 20100120599 | Lid Separation Device and Methods - The present invention includes a device comprising a lid and a vessel forming an interface that allows transfer of waste from a purification chamber to a waste chamber and method for purification of an analyte are disclosed wherein a supernatant is separated from a solid phase to which an analyte is bound by centrifugation via the lid of the device. The present invention also includes methods for use of the device. | 05-13-2010 |
| 20100114507 | METHOD FOR GRAVIMETRIC VOLUME DETERMINATION - A method of gravimetric volume determination is described in which a load cell can be calibrated either outside of an analytic analyzer for gravimetric calibration of said analyzer, or in a fully automated manner inside said analyzer. | 05-06-2010 |
| 20100100363 | Determination of Melting Temperatures by Equation-Less Methods - Numerical determinations of the first derivatives of a melt curve data set are made. A baseline is determined for the first derivative values and the baseline is subtracted from the first derivative values to produce modified first derivative values. A first maximum value of the modified first derivative values is determined and said first maximum value represents a melting temperature Tm of a DNA sample. A model function, such as a Gaussian Mixture Model (GMM) function, with parameters determined using a Levenberg-Marquardt (LM) regression process can also be used to find an approximation to the first derivative curve. The maximum values of the numerically determined first derivative values are used as initial conditions for parameters of the model function. The determined parameters provide one or more fractional melting temperature values, which can be returned, for example, displayed or otherwise used for further processing. | 04-22-2010 |
| 20100100332 | DETERMINATION OF MELTING TEMPERATURES BY EQUATION-LESS METHODS - Numerical determinations of the first derivatives of a melt curve data set are made. A model function, such as a Gaussian Mixture Model (GMM) function, with parameters determined using a Levenberg-Marquardt (LM) regression process is used to find an approximation to the first derivative curve. The maximum values of the numerically determined first derivative values are used as initial conditions for parameters of the model function. The determined parameters provide one or more fractional melting temperature values, which can be returned, for example, displayed or otherwise used for further processing. | 04-22-2010 |
| 20100099110 | Allele-Specific Amplification - The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3′-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3′-terminal nucleotide. | 04-22-2010 |
| 20100097231 | Process and System for Measuring Liquid Volumes and for Controlling Pipetting Processes - The instant invention provides a methods and a system for measuring liquid volumes and controlling a pipetting process. | 04-22-2010 |
| 20100093041 | Method for the Prevention of Carryover Contamination in Nucleic Acid Amplification Technologies - An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity. | 04-15-2010 |
| 20100092956 | Analytical Processing and Detection Device - An analytical processing and detection device is described which comprises a rack capable of holding at least one reaction receptacle, at least one magnetic unit for exerting a magnetic field on at least one reaction receptacle comprising magnetic particles in a fluid, wherein said at least one magnetic unit is reversibly or irreversibly connected to said rack, and wherein said magnetic field causes the magnetic particles to be sequestered to the side walls of said at least one reaction receptacle, and a detection unit for detecting a signal in the fluid in said reaction receptacle. | 04-15-2010 |
| 20100086913 | Methods of Identification Using Methylation of CPG - The present invention relates to the materials and methods for the identification of methylated nucleotides in samples of genomic DNA. The present invention also relates to methods of diagnosis of specific conditions by identification of specific methylated nucleotides. | 04-08-2010 |
| 20100070185 | REAL-TIME PCR ELBOW CALLING BY EQUATION-LESS ALGORITHM - Systems and methods for determining a transition value in a sigmoid or growth curve, such as the end of the baseline region or the elbow value or Ct value of a PCR amplification curve. Numerical determinations of the second derivatives and curvature values of a PCR data set are made. A Gaussian Mixture Model (GMM) function with parameters determined using a Levenberg-Marquardt (LM), or other, regression process is used to find an approximation to the second derivative values and to the curvature values, where the maximum values of the numerically determined second derivative values and/or curvature values are used as initial conditions for parameters of the GMM function. The determined parameters provide fractional Ct values. The Ct value(s) are then returned and may be displayed or otherwise used for further processing. | 03-18-2010 |
| 20100041053 | PROOFREADING PRIMER EXTENSION - The present invention provides for primer extension reactions, including polymerase chain reactions, in which a polymerase having 3′-5′ exonuclease activity edits a primer that is not fully complementary thereby allowing for amplification and detection of target nucleic acids that may have variability in their sequences. | 02-18-2010 |
| 20100041040 | Internally Controlled Multiplex Detection and Quantification of Microbial Nucleic Acids - The present invention relates to new methods and uses for the detection and quantification of microbial nucleic acids employing an internal quantitative reference. Preferred methods are based on the amplification of nucleic acids, preferably the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses. Moreover, an analytical system for advantageously performing the method according to the invention is disclosed. | 02-18-2010 |
| 20100028864 | REAGENTS AND METHODS FOR DETECTING NEISSERIA GONORRHOEAE - This invention provides compositions and methods for detecting | 02-04-2010 |
| 20100025464 | Method and System to Localise and Identify Test Tubes - A method and a system to localise and to identify at least one of a plurality of test tubes in a test tube rack using RFID technology. | 02-04-2010 |
| 20100021923 | METHOD FOR THE EFFICIENCY-CORRECTED REAL-TIME QUANTIFICATION OF NUCLEIC ACIDS - The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes. | 01-28-2010 |
| 20100021352 | Laboratory Device for Processing Samples and Methods Using the Same - The invention relates to devices and methods for the identification of test tubes in a test tube rack using RFID technology. | 01-28-2010 |
| 20100019157 | Excitation and Imaging Optics for Fluorescence Detection - The invention concerns an optical instrument for imaging fluorescence signals from an arrangement of a plurality of individual detection sites, for example the wells of a microtitre plate. In order to improve the light yield of the fluorescence excitation with excitation light as well as the light yield of the detection of the fluorescence signals, an objective array is provided which is arranged in the beam path between the field lens and the detection sites and comprises a field lens array with field lens array elements and a pupil lens array with pupil lens array elements. In order to improve the channel separation and suppress interfering light the objective array can comprise a diaphragm array with in each case two diaphragm openings per detection site. | 01-28-2010 |
| 20100003683 | Disposable Device for Analyzing a Liquid Sample Containing a Nucleic Acid With a Nucleic Acid Amplification Apparatus - The invention is directed to a disposable sample holding and processing device ( | 01-07-2010 |
| 20090317899 | Handling Kit for Analyzing a Liquid Sample by Nucleic Acid Amplification - The invention refers to a handling kit for analyzing a liquid sample, especially by nucleic acid amplification, comprising a disposable sample holding and processing device ( | 12-24-2009 |
| 20090317303 | FLEXIBLE DISPOSABLE TIP INTERFACE - A disposable pipette tip for releasably connecting with a pipette shaft is described, comprising an elongated flexible tubular interface having a central axis for connecting the pipette tip to the pipette shaft and an elongated tubular part extending from a distal tip opening to said tubular interface, wherein said elongated flexible tubular interface has a strain of more than of more than about 20% and wherein the extensibility of said elongated flexible tubular interface is reversible. | 12-24-2009 |
| 20090306360 | 2-AZAPURINE COMPOUNDS AND THEIR USE - Within oligonucleotides, 2-azapurine and especially 2-azaadenine bases form specifically base pairs with guanine. This base pair is of analogous stability as an adenine-thymine but less stable than a guanine-cytosine base pair. Therefore, the incorporation of 2-azaadenine residues into oligonucleotides instead of cytosine leads specifically to hybridization complexes with nucleic acids with homogenous stability. This is useful for the adaptation of the stabilities of different oligonucleotide sequences in all kinds of hybridization techniques, for example in oligomer chip technology. | 12-10-2009 |
| 20090291487 | Thermocycling Device - The invention relates to a thermocycler module for heating and/or cooling of a thermocycling device comprising a thermal switch, a heating block and a heat sink. The thermal switch comprises a thermoconducting liquid, e.g. a magnetic fluid or a liquid metal, and a stimulating unit, e.g. a magnetic unit or a Lorentz-force unit, for moving the thermoconducting liquid. The movement of the thermoconducting liquid provides, in an on-state of the thermal switch, a thermal connection between the heating block and the heat sink. | 11-26-2009 |
| 20090287754 | SYSTEMS AND METHODS FOR STEP DISCONTINUITY REMOVAL IN REAL-TIME PCR FLUORESCENCE DATA - Systems and methods for removing jump discontinuities in PCR or growth data. A first approximation to a curve that fits a received data set is determined by applying a non-linear regression process to a non-linear function that models the data set to determine parameters, including a step discontinuity parameter, of the non-linear function. One example of a non-linear function is a double sigmoid equation. A second approximation to a curve that fits the data set is also determined by applying a regression process to a second non-linear function to determine parameters, including a step discontinuity parameter, of the second function. One of the first or second approximations is then selected based on an information coefficient determined for each of the first and second approximations. If a confidence interval calculated for the step discontinuity parameter includes the value zero, no step correction is made. If the confidence interval does not include the value zero, then a step correction is made. If a step correction is made, the portion of the data curve prior to the step change is replaced with appropriate portion of the selected approximation to produce a shift-corrected data set. In certain aspects, the portion of the data curve up to the first point after the step change is corrected. In certain aspects, if the approximation does not satisfy a goodness of fit criterion, no step correction is made. The shift-corrected data set is returned and may be displayed or otherwise used for further processing. | 11-19-2009 |
| 20090280539 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 11-12-2009 |
| 20090263813 | RIBONUCLEOTIDE TAG NUCLEIC ACID DETECTION - The present application provides polynucleotides comprising 5′-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5′-tail sequence segments. Cleavage of amplification products at the bond immediately 3′ to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid. | 10-22-2009 |
| 20090176236 | Compositions and Methods for Detecting Certain Flaviviruses, Including Members of the Japanese Encephalitis Virus Serogroup - The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St. Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, and Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3′ untranslated region of the viral genomes to be detected. | 07-09-2009 |
| 20090162846 | SENP1 as a marker for cancer - The present invention provides methods of detecting cancer cells by detecting the quantity of SENP1 and/or telomerase in a sample. | 06-25-2009 |
| 20090155802 | Mutant DNA Polymerases with Improved Pyrophasphorolysis Activated Polymerization (PAP) Ability - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 06-18-2009 |
| 20090155773 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 06-18-2009 |
| 20090148891 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 06-11-2009 |
| 20090142810 | 2'-Terminator Nucleotide-Related Methods and Systems - The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media. | 06-04-2009 |
| 20090137000 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 05-28-2009 |
| 20090120104 | THERMAL BLOCK UNIT - The present invention discloses a thermal block unit for thermal treatment of samples comprising temperature regulating units, temperature sensors for measuring temperature at different locations of the thermal block unit, a converter for converting signals from the temperature sensors into digital signals and a thermal block interface for communicating with an instrument. | 05-14-2009 |
| 20090119020 | PCR ELBOW DETERMINATION USING QUADRATIC TEST FOR CURVATURE ANALYSIS OF A DOUBLE SIGMOID - Systems and methods for determining whether the data for a growth curve represents or exhibits valid or significant growth. A data set representing a sigmoid or growth-type curve, such as a PCR curve, is processed to determine whether the data exhibits significant or valid growth. A first or a second degree polynomial curve that fits the data is determined, and a statistical significance value for the curve fit is determined. If the significance value exceeds a significance threshold, the data is considered to not represent significant or valid growth. If the data does not represent significant or valid growth, the data set may be discarded. If the significance value does not exceed the significance threshold, the data is considered to represent significant or valid growth. If the data set is determined to represent valid growth, the data is further processed to determine a transition value in the sigmoid or growth curve, such as the end of the baseline region or the elbow value or Ct value of a PCR amplification curve. | 05-07-2009 |
| 20090118132 | Classification of Acute Myeloid Leukemia - The present invention relates to rapid and reliable approaches to leukemia prognostication. In addition to methods, the invention also provides related kits and systems. | 05-07-2009 |
| 20090098532 | Probes and Methods for Hepatitis C Virus Typing Using Single Probe Analysis - This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV genotypes (for example, selected from genotypes 1, 2, 3, 4, 5 or 6), or assign an HCV isolate to one of at least six subtypes (for example, selected from subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a), where the methods of the invention use only a single typing probe to make the HCV type assignment. | 04-16-2009 |
| 20090081661 | Alkyl amines improve detection of components of formaldehyde-fixed biological samples - Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization. | 03-26-2009 |
| 20090070049 | METHOD FOR MONITORING A FLUID TRANSFER PROCESS - The present invention relates to a method for monitoring a fluid transfer process, including the steps: providing an allowable pressure profile; detecting a pressure occurring in the course of the fluid transfer process; comparing detected pressure with the allowable pressure profile and signaling an error, if the detected pressure is not within the allowable pressure profile. The allowable pressure profile is defined by interpolation points, the interpolation points being based on a probability function representing a family of pressure courses of a plurality of fluid transfer processes. The allowable pressure profile can be divided into at least two distinct process sections, each section corresponding to a distinct process phase of the fluid transfer process. The probability function is calculated from a family of test pressure curves and reflects the statistical behavior of the pipette system. The present invention further relates to a computer readable medium for storing interpolation point information as well as to a kit-of-parts comprising a device implementing the inventive method, together with a computer readable medium for storing interpolation point information. | 03-12-2009 |
| 20090068747 | APPARATUS FOR EMITTING AND DETECTING LIGHT IN A NUCLEIC ACID AMPLIFICATION REACTION - Subject of the present invention is to provide an apparatus, an instrument, and a method particularly useful in multiplex PCR applications permitting short sample measuring times of many samples combined with high sensitivity. | 03-12-2009 |
| 20090053720 | SUPPRESSION OF AMPLIFICATION USING AN OLIGONUCLEOTIDE AND A POLYMERASE SIGNIFICANTLY LACKING 5'-3' NUCLEASE ACTIVITY - Methods and compositions for amplification of a target sequence by suppressing amplification of related sequences are provided. | 02-26-2009 |
| 20090035779 | SYSTEMS AND METHODS FOR DETERMINING CROSS-TALK COEFFICIENTS IN PCR AND OTHER DATA SETS - Systems and methods for determining cross-talk coefficients in curves, such as sigmoid-type or growth curves, and PCR curves and nucleic acid melting curves in particular, as well as for applying the cross-talk coefficients to produce cross-talk corrected data sets using a linear subtractive model. Cross-talk signal coefficients are determined using cross-talk data acquired across the entire signal acquisition range. Analyzing across all of the signal curve data provides for a more robust cross-talk correction across the entire data acquisition range. A linear subtractive model is used to correct data sets having cross-talk components. | 02-05-2009 |
| 20080293071 | Sequencing and Genotyping Using Reversibly Terminating Nucleotides - The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2′ position. | 11-27-2008 |
| 20080257039 | Device and Method for the Gravimetric Determination of Liquid Volumes and Analyzer System Comprising Such a Device - A device for the gravimetric volume determination of liquid volumes. The device comprises a housing ( | 10-23-2008 |
| 20080254468 | Micro-Fluidic Temperature Driven Valve - Subject of the present invention is a micro-fluidic device for the use in an apparatus for analyzing a liquid sample by nucleic acid amplification, an apparatus for analyzing a liquid sample by nucleic acid amplification, a method for analyzing a liquid sample and a method for amplifying nucleic acids in a liquid. | 10-16-2008 |
| 20080206111 | Device with a Compressible Matrix Having Homogeneous Flow Profile - A fluidic device containing a flow through chamber comprising an inlet port, an outlet port, and a fluid permeable, compressible matrix located in the chamber between said inlet port and said outlet port, and said compressible matrix having a higher flow resistance at higher compressions and a plurality of flow paths leading from said inlet port to said outlet port through said matrix comprising shorter and longer length flow paths, wherein said matrix is compressed in at least one dimension, being more strongly compressed along the shorter length flow paths than along the longer length flow path. | 08-28-2008 |
