| PERKINELMER HEALTH SCIENCES, INC. Patent applications |
| Patent application number | Title | Published |
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| 20130030184 | ISOTOPICALLY LABELED CHEMICALLY STABLE REAGENTS AND PROCESS FOR THE SYNTHESIS THEREOF - A radioisotope labeled reagent includes a compound having the general formula (I), | 01-31-2013 |
| 20130017974 | METHODS AND COMPOSITIONS RELATING TO MULTIPLEX GENOMIC GAIN AND LOSS ASSAYS - Compositions and methods are provided for detecting genomic DNA gain and loss. Embodiments of inventive compositions and methods include composite nucleic acid probes which specifically hybridize to two or more genomic loci in a genomic region of a reference genome for detection of genomic gain and/or loss in a subject genome. In some embodiments, a substrate-attached composite nucleic acid probe is provided which includes a mixture of separate populations of beads having attached DNA probes wherein all of the beads are identically encoded and wherein each individual bead has exclusively DNA derived from one source, such as a particular large insert vector containing chromosomal DNA, or amplicons generated by amplification of DNA derived from a large insert vector containing chromosomal DNA. | 01-17-2013 |
| 20120309096 | DETECTING SUCCINYLACETONE - This invention relates, inter alia, to detecting and/or measuring succinylacetone and one or more additional biological analytes using mass spectrometry. | 12-06-2012 |
| 20120223244 | ELECTROSTATIC LENSES AND SYSTEMS INCLUDING THE SAME - A system includes an electrostatic lens in a path between a charged particle source and a detector. The electrostatic lens includes: a first electrode having a first aperture in the path aligned with a first axis; a second electrode in the path between the first electrode and the detector, having a second aperture in the path and aligned with a second axis that is parallel to the first axis and displaced from the first axis along a first direction; a third electrode in the path between the first electrode and the second electrode; and a potential generator coupled to the electrodes. During operation, the potential generator applies potentials to the first, second and third electrodes so that the electrostatic lens directs a beam of charged particles from the source propagating along the first axis to propagate along the second axis. | 09-06-2012 |
| 20120190043 | ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS - An inventive substrate is provided which includes a substrate compound of formula A-B | 07-26-2012 |
| 20120180579 | LARGE VOLUME PIPETTE TIP FOR LOADING IN AN AUTOMATED LIQUID HANDLER - A pipette tip having lower, upper and central portions and a bore extending therethrough. The lower portion is elongated and projects in a first direction from the central portion, and is sized and shaped for insertion into a test-tube. The bore is adapted for aspirating and dispensing a large volume of the liquid. The upper portion projects from the central portion in a second direction opposing the first direction, and its terminal end is sized and shaped to sealingly engage an automated pipetting system. The central portion and a corresponding portion of the bore are enlarged with respect to both the upper and lower portions to allow the pipette tip to hold the large volume of the liquid. The central portion can have a cross-section substantially rectangular in shape. The cross-section can have a height at least substantially similar to the diameter of the lower portion of the pipette tip and a width significantly greater than the diameter of the lower portion of the pipette tip. | 07-19-2012 |
| 20120169237 | HOLLOW CATHODE LAMP ELAPSED TIME RECORDING SYSTEM - A hollow cathode lamp is described with an end cap, an anode, and a cathode. A data storage device is part of the end cap and communicates data to and from a computing device. The data communicated with the computing device may include identification information and usage information corresponding to the hollow cathode lamp. Additionally, a method is described that includes activating a power supply to a hollow cathode lamp and communicating data with a memory device located in the hollow cathode lamp. The data communicated with the memory device includes usage information about an amount of time the hollow cathode lamp has been in use. | 07-05-2012 |
| 20120126114 | Magnetic Sector Mass Spectrometry Based Multi-Parametric Particle Analyzer - An analytical instrument has a sample introduction system for generating a stream of particles from a sample and an ionization system for receiving the particles. The ionization system is operable to atomize the particles received from the sample introduction system and ionize atoms from the atomized particles. The instrument has an ion pretreatment system and a magnetic sector mass analyzer comprising an array detector. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted to detect a transient signal of at least one element from individual particles from said stream by performing mass analysis on the ions from the atomized particles. The magnetic sector mass analyzer is adapted determine an amount of said at least one element from an individual particle using the transient signal detected during mass analysis of the ions from said individual particle. | 05-24-2012 |
| 20120122131 | SIMULTANEOUS DETECTION OF METABOLIC ENZYME ACTIVITY AND METABOLITE LEVELS - Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) a metabolically indicative enzyme and (ii) a metabolic analyte, with a substrate for the enzyme to produce a reaction admixture, under conditions wherein the enzyme is capable of acting on a corresponding substrate to generate a product, and wherein a protease inhibitor is present; (b) contacting the reaction admixture with a reagent that inhibits the ability of the enzyme to act on a corresponding substrate, wherein the metabolic analyte and the product are soluble in the reagent; to produce a test sample and (c) determining the presence or amount of the metabolic analyte and the product contained in the test sample using mass spectrometry, wherein a determined presence or amount of the metabolic analyte and the product correlates with presence or absence of the metabolic disorder. | 05-17-2012 |
| 20120091331 | MULTIMODE CELLS AND METHODS OF USING THEM - A mass spectrometer is provided that is configurable for operation in both a Kinetic Energy Discrimination (KED) mode and a dynamic reaction cell (DRC) mode. To operate in the KED mode, a collision cell can be filled with a quantity of the inert gas, and an energy barrier can be formed between the collision cell and a downstream mass analyzer. To operate instead in the mode, the collision cell can be filled with a quantity of gas that is reactive with the interferer ions. | 04-19-2012 |
| 20120018632 | SINGLE AND MULTIPLE OPERATING MODE ION SOURCES WITH ATMOSPHERIC PRESSURE CHEMICAL IONIZATION - An Atmospheric Pressure Chemical Ionization (APCI) source interfaced to a mass spectrometer is configured with a corona discharge needle positioned inside the APCI inlet probe assembly. Liquid sample flowing into the APCI inlet probe is nebulized and vaporized prior to passing through the corona discharge region all contained in the APCI inlet probe assembly Ions produced in the corona discharge region are focused toward the APCI probe centerline to maximize ion transmission through the APCI probe exit. External electric fields penetrating into the APCI probe exit end opening providing additional centerline focusing of sample ions exiting the APCI probe. The APCI probe is configured to shield the electric field from the corona discharge region while allowing penetration of an external electric field to focus APCI generated ions into an orifice into vacuum for mass to charge analysis. Ions that exit the APCI probe are directed only by external electric fields and gas flow maximizing ion transmission into a mass to charge analyzer. The new APCI probe can be configured to operate as a stand alone APCI source inlet probe, as a reagent ion gun for ionizing samples introduced on solids or liquid sample probes or through gas inlets in a multiple function ion source or as the APCI portion of a combination Electrospray and APCI multiple function ion source. Sample ions and gas phase reagent ions are generated in the APCI probe from liquid or gas inlet species or mixtures of both. | 01-26-2012 |
| 20120004413 | ISOTOPICALLY LABELED CHEMICALLY STABLE REAGENTS AND PROCESS FOR THE SYNTHESIS THEREOF - A radioisotope labeled reagent includes a compound having the general formula (I), | 01-05-2012 |
| 20110313713 | DIFFERENTIAL SCANNING CALORIMETRY AND CALIBRATION METHODS FOR USE THEREWITH - Certain embodiments herein are directed to a differential scanning calorimeter comprising a sample holder thermally coupled to a first furnace, a reference holder thermally coupled to a second furnace, and a processor electrically coupled to the first furnace and the second furnace, the processor configured to receive data during a scan of a sample to provide a heat flow trace and further configured to subtract a calculated baseline from the heat flow trace, the calculated baseline comprising the sum of an isothermal baseline function, a scanning baseline function and a transient baseline function. Calibration methods are also described. | 12-22-2011 |
| 20110309244 | MULTIPOLE ION GUIDE ION TRAP MASS SPECTROMETRY WITH MS/MSN ANALYSIS - A Time-Of-Flight mass analyzer includes a multipole ion guide located in the ion flight path between the ion source and the flight tube of the Time-Of-Flight mass analyzer. In one preferred embodiment, a Time-Of-Flight (TOF) mass analyzer is configured such that a multipole ion guide is positioned in the ion path between the ion source and the ion pulsing region of the TOF mass analyzer. The multipole ion guide electronics and the ion guide entrance and exit electrostatic lenses are configured to enable the trapping or passing through of ions delivered from an atmospheric pressure ion source. The ion guide electronics can be set to select the mass to charge (m/z) range of ions which can be successfully transmitted or trapped in the ion guide. More than one set of m/z values can be selected using techniques such as notch filtering with resonant frequency ion ejection of unwanted m/z values. All or a portion of the ions with stable ion guide trajectories in transmission or trapping mode can then undergo Collisional Induced Dissociation (CID) using one of at least three techniques. During the ion fragmentation step the multipole ion guide AC and DC electric potentials are set to transmit or trap all or a portion of the fragment ions produced by the CID process. All or a portion of the parent and fragment ion population are delivered from the multipole ion guide to the pulsing region of Time-OF-Flight mass analyzer for mass analysis. After the first ion fragmentation step, the multipole ion guide AC and DC electric potentials can again be set to select a narrow m/z range to clear the ion guide in trapping mode of all but a selected set of fragment ions. The m/z selection and ion fragmentation step can be repeated a number of times with mass analysis occurring at the end of all the MS/MS | 12-22-2011 |
| 20110309243 | Atmospheric Pressure Ion Source for Mass Spectrometry - An apparatus for generating ions includes an Electrospray ionization source configured to provide a spray of charged droplets from a sample solution during operation of the apparatus; an atmospheric pressure chemical ionization (APCI) source including a corona discharge needle configured to produce a corona discharge that further ionizes the spray during operation of the apparatus; and a gas delivery system configured to deliver a gas flow to the corona discharge needle during operation of the apparatus, wherein the gas flow comprises a reagent ion gas which facilitates ionization of the spray by the corona discharge. | 12-22-2011 |
| 20110306084 | Tritiated Planar Carbon Forms - Tritiated planar carbon forms and their production are provided. Methods are provided for the stoichiometrically controlled labeling of planar carbon forms capitalizing on normal flaws of carboxylic acids ubiquitously present in commercial preparations of these planar carbon forms. Alternative methods include generation of a metallated intermediate whereby a metal is substituted for hydrogen on the carbon backbone of a planar carbon form. The metalized intermediate is then reacted with a tritium donor to covalently label the planar carbon form. The tritiated planar carbon forms produced are useful, for example, for determination of a biological property or environmental fate of planar carbon forms. | 12-15-2011 |
| 20110303840 | MULTIPOLE ION GUIDE ION TRAP MASS SPECTROMETRY WITH MS/MSN ANALYSIS - A method includes directing ions from an atmospheric pressure ion source to a first ion guide; directing ions in the first ion guide to a second ion guide, the second ion guide being a multipole ion guide extending along an axis; periodically directing ions along the axis; receiving at least some of the ions in a time-of-flight analyzer; accelerating the ions in the time-of-flight mass analyzer orthogonal to the axis; and detecting the accelerated ions. | 12-15-2011 |
| 20110297822 | METHODS AND INTERFACES FOR SINGLE AND MULTIDIMENSIONAL SEPARATIONS FOR CHARACTERIZATION AND/OR IDENTIFICATION OF MOLECULES BY MASS SPECTROMETRY - The present invention relates a use of the electrocapture-based separation technology combined with mass spectrometrical fragmentation methods, e.g. sequence of polypeptides by collision-induce dissociation mass spectrometry, for the identification and/or characterization molecules of interest. It also relates the combination of the electrocapture-base separation technology with other liquid separation methods, as e.g. liquid chromatography, in order to achieve multi-dimensional separations prior mass spectrometrical analysis. In addition, it relates physical interfaces between electrocapture-based separations and different types mass spectrometers for on-line or off-line analysis, as well as the coupling of electrocapture-based separations, liquid chromatography and different types of mass spectrometrometers. | 12-08-2011 |
| 20110294227 | METHOD FOR DETERMINING THE RISK OF PREECLAMPSIA USING PIGF-2 AND PIGF-3 MARKERS - The present invention relates to a method for determining the risk of a pregnant woman developing pre-eclampsia. The method comprises i) determining the level of one or more biochemical markers in a sample obtained from a pregnant woman, and ii) comparing the level of the at least one biochemical marker in the sample with the level of the same biochemical marker in a control sample. A difference in the level of the biochemical marker in the sample relative to the control sample is indicative of an increased risk of developing pre-eclampsia. The isoform biochemical markers are preferably P1GF-2 and P1GF-3. The present invention relates also to a method for determining whether a pregnant woman has pre-eclampsia and as well as a kit for assessing the risk or presence of pre-eclampsia. In addition, the invention relates also to a computer program used in these determinations. | 12-01-2011 |
| 20110220789 | Sample Component Trapping, Release, and Separation with Membrane Assemblies Interfaced to Electrospray Mass Spectrometry - The invention provides a method and apparatus for trapping, releasing and/or separating sample components in solution passing through a channel with or without packing material present by passing ion current through the channel driven by an electric field. A portion of the ion current comprises cation and/or anion species generated from second solution flows separated from the sample solution flow path by semipermeable membranes. Cation and/or Anion ion species generated in the second solution flow regions are transferred into the sample solution flow path through ion selective semipermeable membranes. Ion current moving along the sample solution flow path is controlled by varying the composition of the second solutions and/or changing the voltage between membrane sections for a given sample solution composition. The sample composition may also be varied separately or in parallel to enhance trapping, release and/or separation efficiency and range. The invention when interfaced to an Atmospheric Pressure Ion Source, that may include Electrospray Ionization, with mass spectrometric analysis enables independent control of the on-line sample separation process and the Atmospheric Pressure Ion Source or Electrospray ionization processes. | 09-15-2011 |
| 20110201539 | DETECTING SUCCINYLACETONE - This invention relates, inter alia, to detecting and/or measuring succinylacetone and one or more additional biological analytes using mass spectrometry. | 08-18-2011 |
| 20110189780 | DETECTING ISOMERS USING DIFFERENTIAL DERIVATIZATION MASS SPECTROMETRY - Methods of evaluating molecular isomers of branched-chain amino acids are featured. The methods can include: derivatizing one or more molecular isomers of branched-chain amino acids in a sample comprising a branched-chain amino acid labeled with one or more heavy atoms as a first standard; adding, to the sample, after derivatization, a nonderivatized or derivatized branched chain amino acid that is labeled with one or more heavy atoms, as a second standard; evaluating the sample using tandem mass spectrometry; and detecting peaks indicative of derivatized and nonderivatized forms of one or more branched-chain amino acids in the sample. | 08-04-2011 |
| 20110172117 | MULTIPLEXED GENOMIC GAIN AND LOSS ASSAYS - Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA. | 07-14-2011 |
| 20110170095 | DSC-RAMEN ANALYTICAL SYSTEM AND METHOD - A combination DSC testing and Raman spectroscopy system is provided for running investigations on a single sample in the same experiment. A DSC instrument includes a set of optics, which allows an associated Raman unit to emit a pulsed laser that intermittently directs a laser signal to the sample and to collect the Raman signal while simultaneously running a DSC experiment on the same sample. The DSC has a vessel adapted to contain the sample, a thermal analysis environment is adapted to hold the vessel. An associated temperature control apparatus changes the temperature of the analysis environment between temperature endpoints to observe the sample at various transitions. The Raman spectroscopy unit is configured to generate laser pulses which stimulate emission of Raman spectra to provide further information about the sample without introducing excessive noise in the DSC aspect of the investigation. | 07-14-2011 |
| 20110046009 | METHODS FOR DETECTING DNA METHYLATION USING ENCODED PARTICLES - Methods for detecting the methylation status of a target genomic locus are provided. Methods described allow for simultaneous assay of multiple cytosines in a target genomic locus. Assays of multiple cytosines in a target genomic locus provide detection of an aggregate cytosine methylation state of the target genomic locus. Methods to detect methylation of a genomic locus associated with a disease or disorder characterized by aberrant methylation of the genomic locus, such as, but not limited to, Fragile X mental retardation syndrome, Prader-Willi syndrome, Angelman sydrome, Beckwith-Wiedemann syndrome, and Russell-Silverman syndrome, diabetes, cancer, multiple sclerosis or schizophrenia are described herein. | 02-24-2011 |
| 20110014621 | DETECTING MULTINUCLEOTIDE REPEATS - Methods of determining the length of a multinucleotide repeat region in a target nucleic acid are provided herein which include labeling amplified target nucleic acids with a target detection label independent of the number of multinucleotide repeats and a repeat-detection label proportional to the number of multinucleotide repeats, wherein the two types of labels are each independently incorporated in the amplified target nucleic acids during the amplifying or after the amplifying; binding the amplified target nucleic acids to a capture probe specific for the amplified target nucleic acids; detecting the target detection label associated with the capture probe to produce a first signal; detecting the repeat-detection label associated with the capture probe to produce a second signal; and determining a ratio of the first signal and the second signal, wherein the ratio is indicative of the length of the multinucleotide repeat region in the target nucleic acid. | 01-20-2011 |
| 20100228048 | PROCESS FOR PREPARING SUBSTITUTED AROMATIC CARBOXYLIC ACIDS - A process for preparing an aromatic carboxylic acid having a heteroatom containing substituent is provided that includes reaction in a vessel of an aromatic precursor having an aromatic core with at least one heteroatom containing substituent and at least one hydrogen extending from the core, with a haloacetonitrile under reaction conditions to form an aromatic acetonitrile with an acetonitrile moiety. The aromatic acetonitrile is exposed to an oxidizing agent under conditions to convert the acetonitrile moiety to a carboxylic acid group to prepare the aromatic carboxylic acid having the heteroatom containing substituent. | 09-09-2010 |
| 20100227412 | Simultaneous Detection of Estrogen and Non Estrogen Steroids - Methods for determining the amounts of estrogen and non-estrogen steroids in a sample are provided. The methods employ the selective derivatization of estrogen steroids present in a sample and detection of the molecular ions and fragments of the derivatized estrogens and non-estrogen steroids in the sample. The methods provided herein enable the simultaneous quantification of estrogen and non-estrogen steroids in a sample. | 09-09-2010 |
| 20100019142 | YTTRIA-METAL THERMIONIC FILAMENTS - A thermionic electron source comprises a nonlinear metallic substrate, a coating of yttria deposited on the substrate, and a current source configured to drive current through the metallic substrate. | 01-28-2010 |
| 20100015650 | KINASE SUBSTRATES - Tyrosine kinase substrates are described herein that are phosphorylated by many and diverse tyrosine kinases, and are chemically stable relative to co-polymers of poly-EY or poly-EAY having random molecular weights in the range of 20-50 kDa. Tyrosine kinase substrate peptides are provided according to embodiments described herein which include an isolated tyrosine kinase substrate peptide having molecular weight in the range of about 0.5 kD-10 kD. Tyrosine kinase substrate peptides are provided according to embodiments described herein having no more than 50 amino acids. The peptides include 2-25 phosphorylation modules and each phosphorylation module has 2-3 amino acid residues. | 01-21-2010 |
| 20100009373 | METHODS AND COMPOSITIONS RELATING TO MULTIPLEX GENOMIC GAIN AND LOSS ASSAYS - Compositions and methods are provided for detecting genomic DNA gain and loss. Embodiments of inventive compositions and methods include composite nucleic acid probes which specifically hybridize to two or more genomic loci in a genomic region of a reference genome for detection of genomic gain and/or loss in a subject genome. In some embodiments, a substrate-attached composite nucleic acid probe is provided which includes a mixture of separate populations of beads having attached DNA probes wherein all of the beads are identically encoded and wherein each individual bead has exclusively DNA derived from one source, such as a particular large insert vector containing chromosomal DNA, or amplicons generated by amplification of DNA derived from a large insert vector containing chromosomal DNA. | 01-14-2010 |
