20090203897 | Method of Using Polymer Embedded Solid Supports for Small Scale Oligonucleotide Synthesis - A method of synthesizing oligonucleotides is claimed. The method utilizes a synthesis column incorporating a filter-like porous polymer cartridge which contains derivatized Controlled Pore Glass (CPG) or cross-linked Polystyrene (PS) directly embedded into the polymer cartridge. Small-scale synthesis of oligonucleotides of a predetermined sequence may be accomplished by introducing reagents of the phosphoramidite method into the synthesis column. These reagents flow through the cartridge allowing the reaction sequence to take place on the derivatized CPG or PS incorporated within the polymer cartridge. | 08-13-2009 |