On-Chip Biotechnologies Co., Ltd.
|On-Chip Biotechnologies Co., Ltd. Patent applications|
|Patent application number||Title||Published|
|20130302828||METHOD FOR DETECTING LOW CONCENTRATIONS OF SPECIFIC CELL FROM HIGH CONCENTRATIONS OF CELL POPULATIONS, AND METHOD FOR COLLECTING AND ANALYZING DETECTED CELL - Conventional CTC detection methods have been problematic in that 1) there is no technique for automatically determining and counting live CTCs in a brief period of time, 2) no process has been developed for detecting, counting, and thereafter collecting and culturing live CTCs, and 3) there exists no flow cytometer that is contamination free and is capable of measuring an entire sample. Provided is a CTC detection method which comprises a pre-treatment step for concentrating and fluorescence staining CTCs, and a step for identifying and counting CTCs. The pre-treatment step includes attaching magnetic beads to EpCAM antibodies expressed by epithelial cell-derived CTCs and concentrating the CTCs through the use of a magnet, fluorescently labeling an epithelia cell surface marker of the CTCs through the use of EpCAM antibodies or 5E11 antibodies, and performing two types of nuclear staining, one being cell membrane-permeable and the other being cell membrane-impermeable. The identifying and counting step includes evaluating the respective absolute concentrations of live and dead CTCs in a volume of blood by automatically identifying CTCs by the ratio of a plurality of fluorescence signal intensities using a flow cytometer, and differentiating between and counting the live CTCs and the dead CTCs. In the cytometer, an entire liquid-feeding system that includes a flow cell can be replaced for each sample, and the total amount of a liquid sample can be measured.||11-14-2013|
|20120288920||DISPOSABLE CHIP-TYPE FLOW CELL AND FLOW CYTOMETER USING THE SAME - The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.||11-15-2012|
|20110294139||DISPOSABLE CHIP-TYPE FLOW CELL AND FLOW CYTOMETER USING SAME - The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.||12-01-2011|
|20110069311||FLOW CYTOMETER AND FLOW CELL FOR THE SAME - An apparatus and a flow cell for the same, which simultaneously fulfill the requirements of: 1) no need of cleansing a liquid sending system for each measurement; 2) enabling a sample liquid after measurement to be collected without being diluted and free from contamination; and 3) the flow cell being inexpensive are provided for a flow cytometer. Provided are an apparatus for measuring a particle in a liquid, the apparatus having a function that, using a flow channel into which a sample liquid containing a particle is introduced, a pair of flow channels into which a sheath liquid is introduced, the pair of the flow channels being arranged on the opposite sides of the flow channel and joined, and a flow channel in which these flow channels are joined and the sample liquid and the sheath liquid on the opposite sides of the sample liquid flow, applies laser light to a particle flowing in a flow channel in the flow cell, detects scattered light or fluorescence generated from the particle and analyzes the particle, wherein: the flow channels in the flow cell form a structure in which the upstream side and the downstream side of the flow channels are symmetrical to each other; a first liquid storage tank and a second liquid storage tank are formed on the upstream side and the downstream side of the flow cell, respectively; the sheath liquid-introduced flow channels are connected to the first liquid storage tank; the sample liquid-introduced flow channel is connected to a third liquid storage tank inside the first liquid storage tank, the pair of sheath liquid flow channels connected to the first liquid storage tank share a same liquid surface in the first liquid storage tank; the third liquid storage tank is separated from the first liquid storage tank, and has a structure preventing the sheath liquid and the sample liquid from being mixed in the first liquid storage tank; the flow channel in which the sheath liquid and the sample liquid are joined are divided on the downstream side in a pattern symmetrical to the upstream side; the flow channel at the center of the downstream flow channels is connected to a fourth liquid storage tank inside the second liquid storage tank; the separated flow channels on the opposite sides of the center flow channel are connected to the second liquid storage tank; a gas with a constant pressure higher than an atmospheric pressure, the gas being generated outside the flow cell is provided to the first liquid storage tank using a detachable cap structure; the flow rate of the sample liquid is controlled to be constant by controlling the pressure of the gas; the sample liquid is collected into the fourth liquid storage tank on the downstream side; and the sheath liquids are collected into the second liquid storage tank, and a flow cell for the apparatus.||03-24-2011|
Patent applications by On-Chip Biotechnologies Co., Ltd.