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OCEAN UNIVERSITY OF CHINA
| OCEAN UNIVERSITY OF CHINA Patent applications | ||
| Patent application number | Title | Published |
|---|---|---|
| 20120015439 | METHOD FOR THE RECONSTRUCTION OF A TISSUE-ENGINEERED HUMAN CORNEAL ENDOTHELIUM - This invention relates to a method for the reconstruction of a tissue-engineered human corneal endothelium. Human corneal endothelial cells are cultured in vitro to logarithmic growth phase using 20% calf bovine serum-containing DMEM/F12 medium. Trypsin is used to digest epithelial layer of the freeze-dried human amniotic membrane in order to produce denuded human amniotic membrane as scaffold carriers. The scaffold carriers are tiled on the bottom of culture plate wells until they are dried and completely adhered to the bottom of wells. Human corneal endothelial cells at logarithmic growth phase are re-suspended in DMEM/F12 medium containing type-IV collagen and 20% calf bovine serum. Human corneal endothelial cell suspension is subsequently inoculated to amniotic membrane scaffold carriers that are tiled on the bottom of wells in culture plate to launch in vitro culture as well as in vitro reconstruction of tissue-engineered human corneal endothelium. This invention is scientific and rational. The reconstructed tissue-engineered human corneal endothelium can be produced on large scale to satisfy the great demand of tissue-engineered human corneal endothelium in clinical cornea transplantation for primary corneal endotheliopathy therapy. Meanwhile, costs for in vitro reconstruction of tissue-engineered human corneal endothelium and clinical therapy are low. | 01-19-2012 |
| 20120006278 | BREEDING METHOD FOR ORANGE-ADDUCTOR-MUSCLE SCALLOP - The present invention relates to a method in the field of shellfish breeding techniques, for breeding a scallop population that have improved carotenoid content in the orange-red adductor muscles or other muscle tissues. This method includes the steps of selecting the rare individuals that have orange-red adductor muscles from natural scallop populations, growing the selected scallops, inducing the reproduction, artificially fertilization, and finally breeding a scallop population with orange-red adductor muscles which can be expanded for the cultivation on a large scale. Comparing to other known breeding methods, the present method does not use transgenic technologies to include introduce any exogenous genes, thus does not have any bio-safety and ethics issues. All the mutant scallops with orange-red adductor muscles are selected from natural or cultivated populations. After breeding for four generations, the obtained scallops have abundant carotenoid ingredients in their orange-red adductor muscles. The obtained scallop population is genetically stable and has high survival rate, and it thus can be used for cultivation on a large scale. In summary, this breeding method is simple, low cost and the breeding scallop population has high economic and nutritious values. | 01-12-2012 |
| 20110207602 | NANOMETER POWDER CATALYST AND ITS PREPARATION METHOD | 08-25-2011 |