| NORTIS, INC. Patent applications |
| Patent application number | Title | Published |
|---|
| 20110300616 | METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS - A method for the creation of endothelial parent vessels from human vascular endothelial cells in vitro in a culture perfusion device (CPD) including a collagen chamber, inlet ports, a capillary tube, and an outlet port. A collagen solution is injected into the collagen chamber through a syringe needle until the chamber is filled with collagen. The CPD is perfused by filling the inlet ports and sequentially priming the inlet ports, and the outlet ports. A perfusable channel is created in the collagen chamber and a concentrated suspension of endothelial cells is injected into the inlet ports. The endothelial cells are injected into the at least one perfusable channel and incubated to attach to the walls of the perfusable channel. The cells are distributed within the CPD; and perfused to form a parent vessel having homogeneous monolayers of cells. | 12-08-2011 |
| 20100279268 | METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS - A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded | 11-04-2010 |
| 20090317787 | METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS - Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused. | 12-24-2009 |
