Miltenyi Biotec GmbH Patent applications |
Patent application number | Title | Published |
20160047809 | IMMUNOGENIC ANTIGENS FROM ASPERGILLUS FUMIGATUS - The present invention provides the in-vitro use of at least one in-vivo-target antigen of | 02-18-2016 |
20160047808 | DEPLETION OF MOUSE CELLS FOR ISOLATION OF HUMAN CELLS - Object of the invention is a process for depleting host cells from a xenograft of human cells on a murine host characterized in
| 02-18-2016 |
20160017325 | LIPOHILLIC OLIGONUCLEOTIDE ANALOGS - Lipophilic oligonucleotide comprising a phosphate glycerol unit containing at least one aliphatic unsaturated carbon bond according to formula (I), with Oligonucleotide an unmodified or modified nucleic acid of 2-1000 nucleotides in length R=a bond or a linker unit Y═OH, SH or NHR3 X and Z=independently O, S or NR3 R3=hydrogen or branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkyl aryl residue with 10 to 30 carbon atoms R1, R2 branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkylaryl residue with 10 to 30 carbon atoms, with the provisio that at least one of the residues R1 or R2 comprises at least one aliphatic carbon-carbon double bond Use of lipophilic oligonucleotide according to Formula I for drug discovery or for transfection of cells. | 01-21-2016 |
20150344832 | MIXING DEVICE FOR HOMOGENIZATION OF CELL SUSPENSIONS - The invention is directed to a homogenization device for cell suspensions, comprising a first rotating magnet; a shaft on which a propeller mixer is attached which rotates around the longitudinal axis of the shaft and is movable in lateral direction of the axis of the shaft; the propeller mixer being provided with at least two propeller blades of which at least one is provided with a second magnet; wherein the propeller mixer is rotated by magnetic interaction of the second magnet with the first rotating magnet. | 12-03-2015 |
20150328605 | METHOD AND DEVICE FOR SUSPENDING CELLS - The invention is related to a device for suspending particles in a fluid, wherein the mixing device includes a first magnet ( | 11-19-2015 |
20150307900 | AUTOMATED GENERATION OF GENETICALLY MODIFIED STEM CELLS - The invention relates to a process for generation of genetically modified stem cells comprising the steps
| 10-29-2015 |
20150017714 | CENTRIFUGATION CHAMBER WITH GAS PERMEABLE MEMBRANE LAYERS FOR CELL CULTIVATION - The invention is directed to a centrifuge chamber comprising a cylinder having a base plate and a cover plate, a rotational axis with at least one port for input and/or output of liquids, at least one port for input of gases and at least one layer for cell culturing, wherein the layer for cell culturing comprises a gas-permeable membrane on which cells can be cultured and wherein the at least one port for input and/or output of gases is connected to the gas-permeable membrane. | 01-15-2015 |
20140315311 | Method and device for cell modification - The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. Furthermore, the invention relates to a method for modifying cells comprising the steps—introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input/output port, —immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g—maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified. | 10-23-2014 |
20140212961 | Centrifuge For Separating A Sample Into At Least Two Components - The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber. | 07-31-2014 |
20140113315 | Cell separation method using a release system for cell-antibody-substrate conjugates containing a polyethylene glycol spacer unit - The invention is directed to a releasable conjugate comprising a biotinylated ligand having a biotin moiety, a ligand moiety (Ligand | 04-24-2014 |
20140087462 | METHOD FOR POLYCLONAL STIMULATION OF T CELLS BY FLEXIBLE NANOMATRICES - The present invention provides a method polyclonal stimulation of T cells, the method comprising contacting a population of T cells with a nanomatrix, the nanomatrix comprising a) a flexible matrix, wherein said matrix is of polymeric material; and b) attached to said polymeric flexible matrix one or more polyclonal stimulatory agents which provide activation signals to the T cells; thereby activating and inducing the T cells to proliferate; wherein the nanomatrix is 1 to 500 nm in size. At least one first and one second stimulatory agents are attached to the same or to separate flexible matrices. If the stimulatory agents are attached to separate beads, fine-tuning of nanomatrices for the stimulation of the T cells is possible. | 03-27-2014 |
20140073007 | SCREENING AND ENRICHMENT SYSTEM FOR PROTEIN EXPRESSION IN EUKARYOTIC CELLS USING A TRICISTRONIC EXPRESSION CASSETTE - The present invention provides a polynucleotide and a method for screening and enriching a recombinant protein expressed in a eukaryotic cell, the polynucleotide comprising a tricistronic expression cassette comprising a) a promoter, b) a gene of interest (GOI), c) a reporter gene, d) a selection marker gene, e) an internal ribosome entry site (IRES) element, and f) a 2A element. Said method comprises a) transfecting or transducing suitable eukaryotic host cells with the polynucleotides of the present invention, b) culturing the eukaryotic host cells under conditions so as to express the protein of interest, the reporter protein and the selection marker protein in a cell culture selection medium suitable for selecting positively-transfected/transduced cells by means of said selection marker, and c) screening, sorting and/or enriching the cells expressing high levels of protein of interest by means of the reporter protein. | 03-13-2014 |
20130316373 | SEPARATION OF LIVING UNTOUCHED NEURONS - The present invention provides the use of the cell surface antigen CD51 as a negative selection marker for neuronal cells and a method for enrichment, isolation and/or detection of neuronal cells comprising the steps a) contacting a sample containing neuronal cells with an antigen-binding fragment specific for the CD51 antigen coupled to a solid phase, thereby labeling the CD51 positive cells of said sample and b) isolating the non-labeled cells of said sample. | 11-28-2013 |
20130310241 | CENTRIFUGATION CHAMBER WITH DEFLECTORS - A centrifuge chamber for separating a sample into at least two components, including a cylinder having a base plate and a cover plate, and a rotational axis assembly having at least one port for input and/or output of the sample, the port being connected to a tube located on or in the base plate and/or cover plate, and the tube having one or more openings into the centrifuge chamber, where at least one opening of the tube is provided with at least one flat-shaped deflector having a surface lying substantially parallel with the cylinder, and a width at its base of at most 1/10 of the inner circumference of the cylinder. | 11-21-2013 |
20130306576 | STACKABLE CELL STRAINER - A cell strainer for separating particles from a cell suspension, having an upper portion with at least one filter area lying essentially perpendicular to the direction of flow of the suspension and a lower portion adapted to fit into the openings of at least two sample tubes with different sized openings. The lower portion has a first section with shoulders or flanges having the diameter of the opening of a first tube and a second section having an inner and outer wall serving as a receptacle for the neck of a second tube, where the diameter of the first section is larger than the diameter of the second section. | 11-21-2013 |
20130280258 | PROGNOSTIC, DIAGNOSTIC, AND CANCER THERAPEUTIC USES OF FANCI AND FANCI MODULATING AGENTS - Disclosed herein are methods and compositions for the treatment of cancer. In particular, the present invention identifies and characterizes the FANCI polypeptide as a vital component of the Fanconi anemia pathway and discloses inhibitors of FANCI and methods of using same. Such inhibitors are useful in inhibiting DNA damage repair and can be useful, for example, in the treatment of cancer. | 10-24-2013 |
20130008884 | HEATING DEVICE FOR CYLINDRICAL LABORATORY VESSELS - A heating device for single cylindrical laboratory vessels (e.g., laboratory test tubes), the heating device including a clamp-like element using a force-fit to encompass at least a part of each cylindrical laboratory vessel, one or more electrical resistance heating circuits located at the interior of the clamp-like element, and grab handles to open and close the clamp-like element, thereby to insert or release the cylindrical laboratory vessel from the heating device. | 01-10-2013 |
20120273395 | DEVICE AND METHOD FOR SEPARATION OF NEEL- AND BROWN-MAGNETIC PARTICLES - A method and associated device for enrichment of Neél-magnetic particles from a dispersion of Brown-magnetic particles and Neél-magnetic particles. The device and method use ferromagnetic separation particles having a mean diameter of 100 to 250 μm located in a alternating magnetic field. The ferromagnetic separation particles have a magnetically and chemically inert coating. | 11-01-2012 |
20110269152 | Anchoring/capturing system for selecting or analyzing a CHO cell according to a product secreted by the CHO cell - An anchoring/capturing system for selecting or analyzing a CHO cell according to a product secreted by the CHO cell is described. The anchoring/capturing system comprises a first antibody or a first antigen-binding fragment for anchoring to the extracellular surface of the CHO cell, and a second antibody or a second antigen-binding fragment for binding the secreted product. Uses and methods involving the anchoring/capturing system are provided. | 11-03-2011 |
20110097313 | Method for the Identification and Separation of Non-regulatory T-cells from a Mixture of Regulatory T-cells - The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, “latent TGF-beta (LAP)”, GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles. | 04-28-2011 |
20110009837 | DEVICE FOR REMOVING BIOLOGICAL MATERIAL - The invention pertains to a device ( | 01-13-2011 |
20110004952 | METHOD FOR THE SEPARATION OF CELLS - The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells. | 01-06-2011 |
20100261596 | APPARATUS AND METHOD FOR TRANSFERRING ENERGY AND/OR A SUBSTANCE TO ROTATING MEANS - The presently disclosed subject matter proposes an apparatus ( | 10-14-2010 |
20090155126 | Sample chamber for an analyzing device - A device for carrying out chemical and/or biological reactions comprises a sample chamber ( | 06-18-2009 |