| LEICA MICROSYSTEMS CMS GMBH Patent applications |
| Patent application number | Title | Published |
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| 20120134017 | DEVICE FOR FOCUSING A MICROSCOPE OBJECTIVE ON A SAMPLE - A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample. | 05-31-2012 |
| 20120130670 | System and Method for Computer-Controlled Execution of at Least One Test in a Scanning Microscope - The invention relates to a method and a system for central computer controlled execution of at least one test run in a scanning microscope, particularly a confocal microscope, wherein at least one first software module of an application software is tested. The invention achieves the aim by a network made of individual scanning microscope clients and a central server. The clients can be contacted via a network interface and are administered in a central directory in the server. The application software for the individual components of a scanning microscope is made of individual software modules, each associated with a potential test. In order to be able to perform the various tests, the scanning microscope clients have been equipped on the hardware side with additional sensors and components that allow various operating parameters to be determined. | 05-24-2012 |
| 20120120500 | Optical Element for Distributing Light - An optical element ( | 05-17-2012 |
| 20120099190 | SPIM Microscope with a sequential light sheet - A SPIM-microscope (Selective Plane Imaging Microscopy) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. By an illumination optics having a zoom optics that is provided in the beam path of the illumination light beam the focal length of the illumination light beam can be varied. | 04-26-2012 |
| 20120098949 | SPIM MICROSCOPE WITH A STED LIGHT SHEET - A STED-SPIM-microscope (Selective Plane Imaging Microscopy) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. By optionally turning on a STED deactivation light beam the light sheet can optionally be made thinner and therefore the optical resolution can be increased. | 04-26-2012 |
| 20120092479 | METHOD AND APPARATUS FOR THE MICROSCOPIC IMAGING OF A SAMPLE STRUCTURE - A method and an apparatus for imaging a sample structure ( | 04-19-2012 |
| 20120086795 | MICROSCOPE ILLUMINATION SYSTEM, MICROSCOPE AND OBLIQUE INCIDENT ILLUMINATION METHOD - A microscope illumination system includes a first light source configured to provide light along an optical axis and an aperture device including an aperture wheel that is rotatable about an axis of rotation. The aperture wheel includes a plurality of apertures of varying size disposed circumferentially on the aperture wheel. The aperture wheel is rotatable so as to dispose each of the apertures in a position that is centered on the optical axis so as to generate an illumination beam path that extends in a centered relationship with respect to the optical axis, and so as to dispose each of the apertures in a position that is off-center from the optical axis and that is within a defined region around the optical axis so as to generate an illumination beam path that extends off-center from the optical axis for oblique incident illumination. | 04-12-2012 |
| 20110317260 | Autofocusing Device for Microscopes and Suitable Autofocus Aperture Stops - The present invention relates to an autofocus aperture stop ( | 12-29-2011 |
| 20110304900 | ACOUSTO-OPTICAL SYSTEM, MICROSCOPE AND METHOD OF USE OF THE ACOUSTO-OPTICAL SYSTEM - An acousto-optical system is described comprising at least one acousto-optical element having at least one transducer that is attached to a crystal, a driver unit for generating at least one acoustic signal for driving acousto-optical elements modifying light transmitted through the acousto-optical element and comprising at least one digital data processing unit, at least one digital-to-analog converter transforming the digital combination signal into an initial analog driver signal, and an amplifier for amplifying the initial analog driver signal to become said analog electronic driver signal. Further, a microscope and a method of operating the acousto-optical element is are described. Various objectives are achieved like more flexibility, real time compensation for non-linearity and reducing the number, size, costs and energy consumption of electronic components. | 12-15-2011 |
| 20110299156 | METHOD FOR FCS MEASUREMENTS - A method for conducting FCS measurements includes providing a sample volume, emitting a target light having a first wavelength from a target light source, and marking an FCS volume in the sample volume with the target light by directing the target light onto the sample volume. An illuminating light having a second wavelength is emitted from an illuminating light source, the second wavelength being different than the first wavelength, and the illuminating light is directed onto the sample volume. | 12-08-2011 |
| 20110273768 | TUNABLE MULTIPLE LASER PULSE SCANNING MICROSCOPE AND METHOD OF OPERATING THE SAME - A tunable multiple laser pulse scanning microscope and a method of operating the same is described, applying two pulsed laser beams with distinct wavelengths incident on a scanning spot of a sample to be imaged simultaneously or at a specific time delay. The microscope comprises at least two pulsed laser light sources emitting laser light of distinct wavelengths, an acousto-optic tunable filter (AOTF) for tuning at least one of the laser pulses, a delay stage provided upstream of the AOTF, and an actuator for moving delay stage depending on the time delay. As a result, the wavelength of at least one type of pulses is tuned, and the delay between at least two pulses of distinct wavelengths is adjusted. | 11-10-2011 |
| 20110226963 | METHOD AND APPARATUS FOR PERFORMING MULTIPOINT FCS - A method of performing fluorescence correlation spectroscopy with a fluorescence microscope includes selecting an illumination area of a sample, generating an illumination light beam and splitting the illumination light beam into at least three partial beams. The partial light beams are focused onto the selected illumination area using a microscope optical system of the fluorescence microscope so as to excite fluorescent dye particles in the illumination area to fluoresce. Fluorescent light emitted by the dye particles is detected and at least one diffusion coefficient representative of a diffusibility of the fluorescent dye particles is determined based on the detected fluorescent light. | 09-22-2011 |
| 20110222147 | APPARATUS AND METHOD FOR SIMULTANEOUS FLUORESCENCE EXCITATION (2-WAVELENGTHS-IR) - An apparatus in a simultaneous fluorescence excitation microscope is described, allowing simultaneous fluorescence excitation by light of at least two different wavelengths. The apparatus has a laser light source generating a light beam. A first beam splitter splits the light beam into a first partial light beam and a second partial light beam. A wavelength converter converts the wavelength of the first partial light beam. A microscope optical system into which the first partial light beam and the second partial light beam are coupled directs the two partial light beams onto an object to be examined. | 09-15-2011 |
| 20110222054 | Apparatus and Method for Multi-Modal Imaging in Nonlinear Raman Microscopy - An apparatus for the microscopic examination of an object has an illumination system which generates a first illumination light beam and a second illumination light beam. A first polarization filter ( | 09-15-2011 |
| 20110149290 | METHOD FOR EXAMINING AN OBJECT WITH THE AID OF A MICROSCOPE AND A MICROSCOPE FOR EXAMINING AN OBJECT - A microscope for examining an object includes a laser light source generating pulsed light so as to illuminate the object. A measuring system including a detector is adapted to detect detection light coming from the object and the measuring system generates a measurement signal based on the detection light. The microscope includes a programmable integrated circuit including a control element and at least one of a first delay element and a second delay element. The control element is configured to generate a first control signal adapted to control the detector and the measuring system. The control element is further configured to generate a second control signal adapted to control the laser light source. The first and second delay elements are configured to delay the first and second control signals, respectively. | 06-23-2011 |
| 20100315707 | Objective Changer - An objective changer for a microscope is described, comprising a changing device for transferring at least a first objective held in a first objective holder from a first stand-by position along a first transfer path into an operating position where the first objective defines an optical axis and a second objective held in a second objective holder from a second stand-by position along a second transfer path into an operating position where the second objective defines an optical axis. The first and second transfer paths differ from each other but both extend substantially perpendicular to the optical axis at least within a first area of the first transfer path and a first area of the second transfer path. | 12-16-2010 |
| 20100309546 | OBJECTIVE CHANGER AND A MICROSCOPE - An objective changer for a microscope includes a changing device configured to pendulously swing each of a plurality of objectives into a respective operating position near a focal position. | 12-09-2010 |
| 20100271695 | ARRANGEMENT FOR ANALYZING MICROSCOPIC AND MACROSCOPIC PREPARATIONS - A confocal scanning microscope for examining microscopic and macroscopic objects is described. The microscope comprises: a scanning optical system having optical elements imaging the light generated by a laser onto an object to be examined; an objective provided in a working distance of at least 0.4 inches from an object holder; and a zoom optical system that is connected to the scanning optical system such that the light generated by the laser passes first through the scanning optical system, then through the zoom optical system, and is then imaged through the objective onto the object. This microscope achieves that also macroscopic objects can be viewed at a high resolution. | 10-28-2010 |
| 20100232011 | APPARATUS AND METHOD FOR OPERATING AN ACOUSTO-OPTICAL COMPONENT - An apparatus for controlling an acousto-optical component influencing at least one of illumination light and detection light in a microscope is described. The apparatus comprises a radio-frequency generator for supplying the acousto-optical component with a radio frequency. The radio-frequency generator is configured to compensate deviations in the characteristics of the light due to temperature fluctuations in the acousto-optical component by adapting the radio frequency. The apparatus can be operated by generating a control signal for controlling the radio frequency of the radio-frequency generator; measuring the temperature of the acousto-optical component; adapting the control signal depending on the measured temperature; and sending the adapted control signal into the radio-frequency generator for compensating deviations in the characteristics of the light due to temperature fluctuations and can be used in optical coherence tomography, particularly white light interferometry, optical tweezers in lithography, and distance measurement. | 09-16-2010 |
| 20100214654 | APPARATUS FOR THE DETECTION OF LIGHT IN A SCANNING MICROSCOPE - A light detector for use in a line scanning microscope and a microscope comprising such a light detector are described. The light detector comprises—a line array of avalanche semiconductor detectors; and an electronic trigger circuit that is adapted to operate the avalanche semiconductor detectors in at least one of a Geiger mode with internal charge amplification and in a linear mode. The trigger circuit further comprises a parallel counter that is designed to read out in parallel light pulses detected by the avalanche semiconductor detectors. The parallel counter is adapted to accumulate the light pulses detected by the avalanche semiconductor detectors over a preset counting time. | 08-26-2010 |
| 20100171946 | MICROSCOPE AND METHOD FOR TOTAL INTERNAL REFLECTION-MICROSCOPY - A microscope for total internal reflection microscopy. The microscope includes at least one light source configured to provide an illumination light to an illumination beam path for an evanescent illumination of a specimen so as to reflect the illumination light at an interface to the specimen or a specimen cover so as to return reflection light into the illumination beam path, an objective through which the illumination light and detection light are directable, a detection device, and a coupling device. The coupling device includes a mirror disposed in the illumination beam path. The mirror has a reflecting surface and a hole, the hole being configured to pass the illumination light there through so as to couple the illumination light into the illumination beam path. The reflecting surface is configured to couple out at least a part of the reflection light and to direct the coupled-out reflection light to the detection devices so as to enable determination from a beam pat of the coupled-out reflection light, at least one of a quantifiable parameter and a qualifiable parameter of at least one of the evanescent illumination and an evanescent field created in the specimen. | 07-08-2010 |
| 20100160613 | METHOD FOR HIGH SPATIAL RESOLUTION STOCHASTIC EXAMINATION OF A SAMPLE STRUCTURE LABELED WITH A SUBSTANCE - A method for high spatial resolution stochastic examination of a biological sample structure labeled with a labeling substance is described. The method comprises providing a biological sample structure; choosing such a labeling substance that has molecules present in a first state and in a second state, and the first and second states differ from one another in at least one photophysical property such that there is sufficient probability that one portion of the molecules of the substance will be in the first state and another portion of the molecules will be in the second state and within which labeling substance a change of the state of the molecules can occur spontaneously between the two states in both directions; and labeling the biological sample structure with the substance. | 06-24-2010 |
| 20100134793 | OPTICAL EVALUATION METHOD BY MEANS OF LASER PULSES AND CORRESPONDING APPARATUS - An optical evaluation method and an apparatus for performing said method are described. First laser pulses of a first type and second laser pulses of a second type that differs from the first type are sent onto a sample to be examined. The sample is hit with first incident light from the two laser pulses in at least one manner of simultaneously, within a very short time lag between the two laser pulses, and a time-correlated manner of the two laser pulses, thereby generating a first optical signal, and hit with second incident light from the two laser pulses, thereby generating a second optical signal. The generated first and second optical signals are detected with at least one detector; and an electronic difference between the first and second optical signals is generated. | 06-03-2010 |
| 20100124788 | METHOD FOR HIGH SPATIAL RESOLUTION EXAMINATION OF A SAMPLE STRUCTURE LABELED WITH A SUBSTANCE - A method for high spatial resolution examination of a sample structure includes providing a biological structure as the sample structure with a substance capable of being converted from a first state to a second state. The first and second states of the substance differ in at least one photophysical property. The sample structure is labeled by binding a suitable protein tag including a fluorogen activating protein (FAP) to the sample structure or by expressing the protein tag and the sample structure together as a fusion protein. Then the protein tag is bound to the substance. | 05-20-2010 |
| 20100122588 | DEVICE AND METHOD FOR PROVIDING A PREDETERMINABLE CONCENTRATION OF AT LEAST ONE COMPONENT IN A LIQUID MEDIUM - A method and a device for providing a predeterminable concentration of at least one component in a microscopic sample liquid medium are described. The device includes a feeding device for the at least one component. Measurement data are determined, measuring a predeterminable parameter using a microscopic method. The concentration of the at least one component is adjusted or controlled via the feeding device based on the basis of measurement data. | 05-20-2010 |
| 20100118394 | MICROSCOPE FOR CONVENTIONAL FLUORESCENCE MICROSCOPY AND TOTAL INTERNAL REFLECTION MICROSCOPY - A microscope for conventional fluorescence microscopy (epi-fluorescence) and for total internal reflection microscopy, comprising at least one light source ( | 05-13-2010 |
| 20100103253 | METHOD AND MICROSCOPIC SYSTEM FOR SCANNING A SAMPLE - A method for scanning a sample using an electrically or electronically controllable microscope, includes scanning the sample so as to generate a plurality of images of the sample, each of the plurality of images corresponding to at least one of a different region of the sample and a different time. The microscope is controlled via a control computer during the scanning. The plurality of images are analyzed using at least one second computer connected via a network. The plurality of images are classified and/or the scanning is influenced based on the analyzing. | 04-29-2010 |
| 20100073757 | OPTICAL ARRANGEMENT AND METHOD FOR CONTROLLING AND INFLUENCING A LIGHT RAY - An optical arrangement and a related method for operating this optical arrangement are suggested, particularly in microscopes, for use as at least one of a main beam splitter and a beam combiner. One or more light beams can be coupled into the arrangement and at least one of the light beams that were coupled in can be coupled out again after having passed through the optical arrangement. In the path of the coupled in light beams at least one controllable microstructured element is provided, allowing to switch beam paths within the optical arrangement. This allows controlling or influencing the one or more light beams that are coupled out. | 03-25-2010 |
| 20100055296 | COATING INSTALLATION COMPRISING A RADIO DEVICE AND METHOD FOR CONTROLLING AN ACTUATOR OR A HEATER - A deposition apparatus and methods for controlling an actuator and a heating system in a deposition apparatus are proposed. The deposition apparatus comprises a deposition chamber and at least one substrate holder movably arranged within the deposition chamber for holding substrates to be coated. The deposition apparatus shall further comprise radio equipment having at least a first radio device which is connected to the substrate holder and a second radio device which is arranged at least partially outside the deposition chamber. Between the first radio device and the second radio device a radio link is to be established at least temporarily for transferring information. | 03-04-2010 |
| 20100053725 | ACOUSTO-OPTICAL COMPONENT - An acousto-optical component is suggested, in particular for use in the beam path of an optical arrangement, preferably of a microscope, with a crystal for the passage of light. Sound waves are generated via an electrical transducer and passed through the crystal. The sound waves passing through the crystal affect the optical properties of the crystal and therefore the light passing through the crystal. For exerting a variable influence on the light passing through the crystal, the sound waves passing through the crystal, or the acoustic field in the crystal, are/is variable. | 03-04-2010 |
| 20100053719 | OPTICAL SYSTEM FOR MERGING A FIRST AND A SECOND PARTIAL IMAGE BEAM, EACH PROCEEDING FROM A SPECIMEN, INTO A RESULTANT IMAGE BEAM - An optical system is described for merging a first and a second partial image beam emanating from a specimen into a resultant image beam allowing modification of the areal proportion of the respective first or second partial image beam in the resultant image beam. A stop arrangement comprises at least a first and a second movable stop element each comprising at least one stop region adapted to be brought into a working position with the first or second partial image beam. Movably arranged connecting means for connecting the two stop elements are provided to modify the respective areal proportions of the partial image beams in the resultant image beam by movement of the connecting means. | 03-04-2010 |
| 20100027109 | IMMERSION OBJECTIVE, APPARATUS FOR FORMING AN IMMERSION FILM AND METHOD - An immersion objective for microscopic investigation of a specimen is provided wherein an outer lens is disposed in an objective body. A delivery device including a cap that is disposed over the objective body so as to form a space adapted to receive an immersion liquid is also provided. The cap is open in a region of the outer lens so as to form a gap with the outer lens. The cap includes at least one connector configured to provide a continuous supply of the immersion liquid to the space so that the immersion liquid emerges through the gap to a region between the outer lens and at least one of the specimen and a specimen slide. | 02-04-2010 |
| 20100006772 | METHOD AND APPARATUS FOR HIGH-RESOLUTION OPTICAL SCANNING OF A SAMPLE - A method and an apparatus are suggested for high-resolution optical scanning, particularly in a laser scanning fluorescence microscope. A sample to be scanned comprises a first and a second substance that are switchable into a first and second energy state. In the scanning process, excitation, de-excitation and detection for the first substance is carried out at a different point in time than for the second substance. This achieves a high spatial resolution beyond the diffraction limit while at the same time a high level of information is provided with physically simple and economical means. | 01-14-2010 |
| 20090323058 | LASER MICROSCOPE WITH A PHYSICALLY SEPARATING BEAM SPLITTER - The invention proposes a method for imaging at least one microscopic property of a sample and an apparatus with which the proposed method can be carried out. In the method, at least one coherent illumination light with at least one illumination wavelength is produced by means of at least one light source. The illumination light is imaged onto at least one region on or within the sampled. Detection light emitted by the sample is split at least partially into incoherent detection light and into coherent detection light by means of at least on physically separating beam splitter. The coherent detection light is at least partially separated from the coherent illumination light by at least one beam-splitter element. The coherent detection light is detected. The proposed method can be used in particular for investigating the sample by means of coherent anti-Stokes-Raman scattering. | 12-31-2009 |
| 20090213456 | Illumination Module for Evanescent Illumination and Microscope - A microscope with a light source that produces an illumination light beam for evanescently illuminating a sample includes an adjustment mechanism with which the polarization of the illumination light beam may be changed. | 08-27-2009 |
| 20090195867 | INVERTED MICROSCOPE - An inverted microscope for the high-contrast imaging of objects includes multiple objectives configured to be disposable in an imaging beam path. Each objective has a respective objective pupil associated therewith. A lens system is also provided for generating respective intermediate images of the respective objective pupils. A modulator disposing device, which includes a plurality of modulators, is configured to dispose the modulators at predetermined respective locations on an optical axis corresponding to the locations of the respective intermediate images of the respective objective pupils so as to contrast an object image. | 08-06-2009 |
| 20090160994 | SYSTEM FOR RECORDING AND REPRESENTING IMAGES OF PREPARATIONS - A system for acquiring and displaying images of preparations is provided. The system includes a first and second illumination device, each including an illumination source defining a respective illumination beam path amid configured to emit light to illuminate the preparation. A camera and a display for displaying a portion of the preparation are further provided. The preparation can be placed on a stage that is movable in x and y coordinate directions. The frame is also provided with a combining device configured to combine the first imaging beam path and the second imaging beam path, and direct them to the camera. | 06-25-2009 |
| 20090034066 | APPARATUS FOR ADJUSTING A MICROSCOPE STAGE - An apparatus for adjusting a microscope stage. The microscope stage of the apparatus defines a plane on which an object to be examined can be placed. The apparatus further includes a first shaft and a second shaft rotationally coupled to the first shaft. The microscope stage is guided between the first and second shafts such that a rotational movement of at least one of the first and second shafts has a direct effect on the microscope stage. | 02-05-2009 |
| 20080304146 | Microscope System for Fcs Measurements - A microscope system for conducting FCS measurements. The system includes an illuminating light source configured to emit an illuminating light at an illuminating wavelength. A target light source is provided and configured to emit a target light for marking an FCS volume in a sample volume at a target wavelength. The target wavelength differs from the illuminating wavelength. The system further includes a plurality of optical elements configured to direct the illuminating light and the target light onto the sample volume. | 12-11-2008 |
| 20080266659 | Lens for evanescent wave illumination and corresponding microscope - The invention relates to a lens ( | 10-30-2008 |
| 20080198448 | FLUORESCENCE MICROSCOPE HAVING AN ILLUMINATION DEVICE - An arrangement for fluorescence microscopic examination of specimens includes: a fluorescence microscope; an illumination device that includes: a housing including an interface configured to optically couple the housing and the fluorescence microscope; a plurality of light-emitting diodes disposed in the housing; a respective collector disposed downstream of each of the light emitting diodes and configured to generate a directed light flux; and at least one dichroic splitter disposed in the housing, the at least one splitter and the light-emitting diodes being spatially disposed with respect to one another so that the directed light fluxes are combinable via the at least one splitter into a common illumination beam path directed onto the interface; and a logical control device common to the fluorescence microscope and the illumination device. | 08-21-2008 |
