| 20090298046 | Assays for Histone Deacetylase 1/2 Selective Inhibitors - The present invention relates to an assay specific for histone deacetylases HDAC1 and/or 2 inhibitors which comprises: (i) incubating an HDAC1 and/or 2 enzymes(s) together with a protein that contains the SANT and ELM2 regions, found in MTA proteins such as MTA-2, MTA-1, MTA-3 and also found in CoREST, CoREST2, CoREST3 and MI-ER1, in a suitable assay buffer (ii) adding the potential HDAC inhibitor and a suitable substrate and incubating (iii) stopping the incubation and determining the effect the putative HDAC inhibitor has had on enzyme activity by comparison with standards. | 12-03-2009 |
