| 20100311123 | VECTOR FOR EXPRESSING ANTIBODY FRAGMENTS AND A METHOD FOR PRODUCING RECOMBINANT PHAGE THAT DISPLAYS ANTIBODY FRAGMENTS BY USING THE VECTOR - Disclosed are a plasmid vector (pLA-1 or pLT-2) for producing water-soluble light chain antibody fragments (VL+CL), a phagemid vector (pHf1g3T-1 or pHf1g3A-2) having a heavy chain antibody fragments (VH+CH1)-ΔpIII fusion protein expression and genotype-phenotype linkage function, a host transformed using the vectors, and a method of producing and selecting a water-soluble antibody and recombinant phage displaying an antibody from the host. Also, provided are a method of producing a combinatorial phage display combinatorial Fab fragment libraries DVFAB-IL and DVFAB-13 IL by using a dual vector system (DVS-II) and a method of selecting an antigen-specific human Fab fragment from the combinatorial Fab fragment libraries. | 12-09-2010 |
