HUMAN GENETIC SIGNATURES PTY LTD. Patent applications |
Patent application number | Title | Published |
20150086972 | MOLECULAR DETECTION ASSAY - A molecular detection assay including treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample. | 03-26-2015 |
20120021461 | ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION - A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling. | 01-26-2012 |
20110136098 | METHODS FOR SIMPLIFYING MICROBIAL NUCLEIC ACIDS BY CHEMICAL MODIFICATION OF CYTOSINES - A method for detecting a microorganism comprising reducing the complexity of a microbial genome or microbial nucleic acid by generating a simplified form of the microbial genome or microbial nucleic acid in which substantially all of the positions naturally occupied by cytosines are occupied by uracil or thymine; and assaying for a microbial specific nucleic acid in the simplified form of the microbial genome or microbial nucleic acid, wherein presence of the microbial specific nucleic acid is indicative of the microorganism. | 06-09-2011 |
20110003700 | ELIMINATION OF CONTAMINANTS ASSOCIATED WITH NUCLEIC ACID AMPLIFICATION - Use of a non-natural base with an enzyme capable of degrading a nucleic acid containing a non-natural base in an amplification reaction to eliminate carry-over contaminants in the amplification reaction. | 01-06-2011 |
20100304386 | ENZYMES FOR AMPLIFICATION AND COPYING BISULPHITE MODIFIED NUCLEIC ACIDS - The invention relates to the use of enzymes for copying or amplifying bisulphite modified or treated nucleic acids, wherein the enzymes are more effective in copying or amplifying the nucleic acid compared with native Taq polymerase under substantially the same conditions. | 12-02-2010 |
20100286379 | BISULPHITE TREATMENT OF RNA - The invention relates to a method for bisulphite treating RNA comprising reacting RNA with a bisulphite reagent at 50-90° C. for 5-180 minutes so as to form treated RNA and recovering the treated RNA. | 11-11-2010 |
20100221785 | Isothermal Strand Displacement Amplification Using Primers Containing a Non-Regular Base - The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non-regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling. | 09-02-2010 |
20100121056 | PSEUDONUCLEOTIDE COMPRISING AN INTERCALATOR - The present invention relates to intercalator pseudonucleotides. Intercalator pseudonucleotides according to the invention are capable of being incorporated into the backbone of a nucleic acid or nucleic acid analogue and they comprise an intercalator comprising a flat conjugated system capable of co-stacking with nucleobases of DNA. The invention also relates to oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudo nucleotide. The invention furthermore relates to methods of synthesising intercalator pseudo nucleotides and methods of synthesising oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudonucleotide. In addition, the invention describes methods of separating sequence specific DNA(s) from a mixture comprising nucleic acids, methods of detecting a sequence specific DNA (target DNA) in a mixture comprising nucleic acids and/or nucleic acid analogues and methods of detecting a sequence specific RNA in a mixture comprising nucleic acids and/or nucleic acid analogues. In particular said methods may involve the use of oligonucleotides comprising intercalator pseudo nucleotides. The invention furthermore relates to pairs of oligonucleotides or oligonucleotide analogues capable of hybridising to one another, wherein said pairs comprise at least one intercalator pseudonucleotide. Methods for inhibiting a DNAse and/or a RNAse and methods of modulating transcription of one or more specific genes are also described. | 05-13-2010 |
20100092972 | ASSAY FOR GENE EXPRESSION - An assay for gene expression comprising treating RNA with an agent such as bisulphate that substantially removes secondary structure of the RNA; and measuring the presence or amount of treated RNA so as to obtain an indication of gene expression. The invention also includes use of oligonucleotide, PNA, LNA or INA probes in the assay. | 04-15-2010 |
20100041013 | ASSAY FOR A HEALTH STATE - The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state. | 02-18-2010 |
20090263909 | TREATMENT OF NUCLEIC ACID - Methods for treating nucleic acid including: (a) providing an alkali environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) removing unwanted reagents or diluents from the treated nucleic acid sample; and (d) carrying out de-sulphonation of the precipitated treated nucleic acid at a temperature from 70° C. to 95° C. by adjusting the precipitated treated nucleic acid to a pH of between 10 and less than 12.5 to remove sulphonate groups present on the treated nucleic acid and obtain a nucleic acid sample substantially free of sulphonate groups. | 10-22-2009 |
20090130657 | AMPLIFICATION BLOCKER COMPRISING INTERCALATING NUCLEIC ACIDS (INA) CONTAINING INTERCALATING PSEUDONUCLEOTIDES (IPN) - An amplification blocker comprising an intercalating nucleic acid (INA) containing two or more internal intercalating pseudonucleotides (IPNs) capable of blocking or reducing nucleic acid amplification. Use of the amplification blocker to block or reduce nucleic acid amplification. | 05-21-2009 |
20090042732 | METHODS FOR SIMPLIFYING MICROBIAL NUCLEIC ACIDS BY CHEMICAL MODIFICATION OF CYTOSINES - A method for simplification of a microbial genome or microbial nucleic acid comprising treating microbial genome or nucleic acid with an agent that modifies cytosine to form derivative microbial nucleic acid and amplifying the derivative microbial nucleic acid to produce a simplified form of the microbial genome or nucleic acid. | 02-12-2009 |
20090029346 | Detection of human papilloma virus - An assay for detecting HPV comprising treating the viral nucleic acid with an agent that modifies cytosine to form derivative viral nucleic acid, amplifying at least a part of the derivative viral nucleic acid to form an HPV-specific nucleic acid molecule, and looking for the presence of an HPV-specific nucleic acid molecule, wherein detection of the HPV-specific nucleic acid molecule is indicative HPV. | 01-29-2009 |