| 20110104692 | Oligonucleotide Primers - Disclosed herein is a method of amplifying a target nucleotide sequence in 16S rRNA or in 16S rDNA that includes (a) contacting a sample comprising a 16S rDNA and/or the reverse transcription product of a 16S rRNA with an oligonucleotide primer comprising the nucleotide sequence of TCC TAC GGG AGG CAG CAG (SEQ ID NO 1) or a nucleotide sequence capable of hybridising under high stringency conditions to the sequence complementary to SEQ ID NO 1 and an oligonucleotide primer comprising the nucleotide sequence of CGG TTA CCT TGT TAC GAC TT (SEQ ID NO 2) or a nucleotide sequence capable of hybridising under high stringency conditions to the nucleotide sequence complementary to SEQ ID NO 2; and (b) performing a primer-dependent nucleic acid amplification reaction to amplify the target nucleotide sequence in the 16S rRNA or the 16S rDNA. | 05-05-2011 |
