| GE HEALTHCARE BIO-SCIENCES AB Patent applications |
| Patent application number | Title | Published |
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| 20120093692 | AUTOMATED FLUID HANDLING SYSTEM - Automated fluid handling system comprising a housing ( | 04-19-2012 |
| 20120091063 | METHOD IN A CHROMATOGRAPHY SYSTEM - A method for determining binding capacities of a chromatography column ( | 04-19-2012 |
| 20120090716 | SIMULATOR DEVICE - A simulator device configured to replace a filtration unit in a crossflow filtration system comprises a feed inlet ( | 04-19-2012 |
| 20120079896 | DEVICE FOR REDUCING LOSS OF LIQUID DURING FRACTION COLLECTION - A fraction collector comprising a liquid holding means ( | 04-05-2012 |
| 20120074051 | FLUID DISTRIBUTOR UNIT - A fluid distributor unit comprising a channel system where one or more inlet channels ( | 03-29-2012 |
| 20120073698 | COLUMN PACKING METHOD - A method for packing a media bed in a column ( | 03-29-2012 |
| 20120070060 | METHOD OF DYNAMIC CELL TRACKING IN A SAMPLE - This invention provides a method of dynamic cell tracking in a sample comprising the steps of, generating a correspondence measure between cells in two consecutive time-elapsed images of a sample, evaluating said correspondence measure to generate events linking individual cells in the two images, the events being selected from the group: unchanged, cell removal, cell migration, cell collision and cell division, and providing a tracking output of events linking individual cells in the two images. In the invention, the step of establishing linking events involves verifying the correctness of generated cell collision and cell division events, by calculating an adaptive threshold value for each such event and comparing the threshold value with an event parameter. There is further provided a system for dynamic cell tracking in a sample. | 03-22-2012 |
| 20120061306 | FILTER HOLDER IN A CHROMATOGRAPHY COLUMN - A filter holder to be used in a chromatography column is provided. Said filter holder comprising a filter squeezing part ( | 03-15-2012 |
| 20120043450 | ATTACHMENT ARRANGEMENT FOR A COLUMN HOLDER - An attachment arrangement comprising—two resilient arms ( | 02-23-2012 |
| 20120024070 | PRESSURE SENSOR - Pressure sensor unit for sealed attachment to a fluidic system comprising a mounting member, a membrane cavity extending through the mounting member with an opening in contact with the fluidic system when the sensor unit is attached thereto, a membrane formed at the distal end of the membrane cavity, and a membrane deflection sensor, wherein the membrane is separated from the mounting member by a stress insulating member arranged to isolate the membrane from stress and strain in the mounting member. | 02-02-2012 |
| 20120022239 | PRECIPITATION OF BIOMOLECULES WITH NEGATIVELY CHARGED POLYMERS - The present invention relates to methods of isolating biomolecules. More particularly, the invention relates to methods for isolating antibodies (mAbs) and related proteins including antibody fragments (Fabs) under conditions where they are positive and relatively hydrophobic and will react with negatively charged polymer to form polymer-protein complexes which precipitate. The isolation can be accomplished using inexpensive and biocompatible negatively charged polymers such as polyacrylic acid or carboxymethyldextran polymers of various molecular weights as precipitant. It occurs at relatively high concentrations of polymer (e.g. 10%) and high salt concentration (>50 mM) and conductivity (e.g. > | 01-26-2012 |
| 20120013963 | HOLDER FOR A HOLOGRAPHIC GRATING - An adjustable holder ( | 01-19-2012 |
| 20120010390 | SEPARATION METHOD USING SINGLE POLYMER PHASE SYSTEMS - The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase. With minor or no modification the target containing phase solution can be further processed via standard unit operations such as precipitation, chromatography, and filtration to further purify target and remove any residual polymer. | 01-12-2012 |
| 20110266225 | CHROMATOGRAPHY LIGAND - The present invention relates to a chromatography ligand defined by the following formula R | 11-03-2011 |
| 20110257378 | AQUEOUS TWO PHASE EXTRACTION AUGMENTED PRECIPITATION PROCESS FOR PURIFICATION OF THERAPEUTIC PROTEINS - The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification. | 10-20-2011 |
| 20110240899 | RANDOM ACCESS ROTARY VALVE - A rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face, the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position. Wherein the stator comprises, a main inlet port, a main outlet port, a first component feed port, a first component return port, a second component feed port, a second component return port, and wherein the interconnection paths in the rotor are arranged to: —in a first rotor position interconnect the main inlet port with the main outlet port, —in a second rotor position interconnect the main inlet port with the first component feed port and the first component return port with the main outlet port, —in a third rotor position interconnect the main inlet port with the first component feed port, the first component return port with the second component feed port, and the second component return port with the main outlet port, and —in a fourth rotor position interconnect the main inlet port with the second component feed port and the second component return port with the main outlet port. | 10-06-2011 |
| 20110221899 | LIGHTING APPARATUS AND LIGHTING CONTROL METHOD FOR A CLOSED-CIRCUIT TELEVISION CAMERA, AND LIGHTING CONTROL SYSTEM INTERLOCKED WITH THE CLOSED-CIRCUIT TELEVISION CAMERA - A lighting apparatus and a lighting control method for a closed circuit television camera, which turn on LEDs in accordance with the exposure time of an imaging element in the closed-circuit television camera, and a lighting control system interlocked with the closed circuit television camera, which turns on a plurality of lights in connection with a closed-circuit television system. The lighting apparatus has a lamp module, a lamp module driver, a synchronous signal extraction unit, and a microcomputer which generates a turn-on signal in which pulses corresponding to the shutter exposure time of the imaging element of the camera are repeated using a shutter delay time as a gap, and which drives the lamp module driver in accordance with the generated turn-on signal to turn on the lamp module in the pulse period corresponding to the shutter exposure time. | 09-15-2011 |
| 20110178271 | STERILIZATION METHOD - The present invention relates to a method for sterilization of different materials, especially sensitive material, such as chromatographic media with sensitive ligands. The method for sterilization of a chromatographic separation medium comprises exposing the separation medium to pressurized steam at a temperature of between about 121° C. and about 135° C. | 07-21-2011 |
| 20110166332 | ENHANCED ANTIBODY AGGREGATE REMOVAL WITH CAPTO ADHERE IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - The invention relates to a method for separating at least one non-aggregated protein from a liquid protein preparation by contacting said preparation with a multimodal anion exchanger in the presence of protein-excluded zwitterions at a concentration of 0.25 to 2.5 M. | 07-07-2011 |
| 20110155668 | SEPARATION MEDIUM FOR CHROMATOGRAPHY OF VARIOUS BIOMOLECULES - The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core. | 06-30-2011 |
| 20110152120 | METHOD OF CHARACTERIZING ANTIBODIES - A method of characterizing a population of antibodies to an antigen comprises contacting a sensor surface having the antigen immobilized thereto with a sample containing the antibody population to bind antibodies to the surface, and detecting dissociation of antibodies from the sensor surface during a dissociation phase when sample no longer contacts the surface. Based on the dissociation behaviour, the antibody population is characterized in terms of subpopulations of more and less stable antibodies, respectively. The method may be used to determine the immunogenicity of drug by monitoring the appearance of anti-drug antibodies in a patient over time, and determining any shift in proportions between more stable and less stable antibodies. | 06-23-2011 |
| 20110152118 | METHOD AND SYSTEM FOR DETERMINATION OF MOLECULAR INTERACTION PARAMETERS - A method of determining kinetic parameters for a reversible molecular interaction between a ligand immobilized to a solid support surface and a binding partner to the ligand in solution, comprises sequentially, without intermediate regeneration or renewal of the immobilized ligand, flowing a plurality of fluid volumes containing different known concentrations of the binding partner over the solid support surface, monitoring the momentary amount of binding partner bound to the solid support surface related to time and solution concentration of binding partner and collecting the binding data, and determining the kinetic parameters by globally fitting a predetermined kinetic model for the interaction between the binding partner and the immobilized ligand to the collected binding data, which model allows for mass transport limitation at the solid support surface. An analytical system for carrying out the method, a computer program, a computer program product and a computer system for performing the method are also disclosed. | 06-23-2011 |
| 20110147311 | CHROMATOGRAPHY COLUMN AND MAINTENANCE METHOD - A method of maintenance of a chromatography column is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. The provision of guide elements which can be reversibly attached to the column facilitates removal or insertion of column components. | 06-23-2011 |
| 20110142379 | THREE-DIMENSIONAL PLASTIC BAG AND PRODUCTION THEREOF - The present invention relates to an insert for reinforcement of bags made of plastic film, comprising a star-like plastic part having at least three jaws, wherein the insert is adapted for insertion and heat-sealing at a junction inside a three-dimensional bag where at least three plastic films meet, so that each jaw extends in a direction between two films. The invention also relates to a three dimensional bag incorporating such inserts and a method of producing such a bag. | 06-16-2011 |
| 20110139303 | FRACTION COLLECTOR - A fraction collector where fractions of liquid are sequentially dispensed from a dispensing means into a plurality of receptacles arranged in a cassette in the fraction collector, by displacing the dispensing means relative to the receptacles, the receptacles being arranged at predefined positions with respect to the cassette and the cassette being arranged at a predetermined position with respect to the fraction collector, wherein the cassette is retained at the predefined position with respect to the fraction collector by a magnetic arrangement. | 06-16-2011 |
| 20110124106 | SEPARATION DEVICE - A separation device for separating a wanted end product from a liquid sample comprises a container ( | 05-26-2011 |
| 20110123819 | METHOD FOR STERILIZATION OF CHEMICALLY ACTIVATED SOLID SUPPORT MATERIALS - The present invention relates to a method for sterilization of different materials, especially sensitive material, such as chemically activated solid support material. The method for sterilization of a chemically activated solid support material comprises exposing the solid support material to pressurized steam at a temperature of between about 121° C. and about 135° C. Also provided are sterile, activated solid support material to produced according to the present method, including chromatographic separation medium made thereof. | 05-26-2011 |
| 20110118442 | CHROMATOGRAPHY LIGAND - The present invention relates to a chromatography ligand defined by the following formula R | 05-19-2011 |
| 20110114488 | ELECTROPHORETIC SUPPORT - The present invention relates to electrophoresis and in particular low fluorescent electrophoretic supports for hydrogels used for separation of fluorescence labelled biomolecules. More particularly, the invention relates to use of a polymer having the following formula: | 05-19-2011 |
| 20110112276 | MUTATED IMMUNOGLOBULIN-BINDING PROTEIN - The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine. | 05-12-2011 |
| 20110097464 | METHOD FOR LIQUID PROCESSING - The present invention relates to liquid clarification and stabilization. More closely, the invention relates to both clarification by removal of suspended particles, as well as stabilization against formation of non-microbial haze via reduction of haze forming substances in various liquids. The haze forming substances are proteins and polyphenol tannins which occur in various plant related fluids such as beer wine, juices, flavorings, plant extracts, and even bioprocess streams. The method of the invention accomplishes both size based removal of colloidal non-haze related particles as well as adsorption based removal of haze forming substances without a need for added flocculants. The to method of the invention utilizes hydrophilic surfaces for adsorption of haze forming substances with such surfaces presented by materials arranged in manner so that size based exclusion of suspended particles is also achieved. | 04-28-2011 |
| 20110077766 | AUTOMATED COLUMN PACKING METHOD - A method for automatically without any required manual interaction packing a media bed in a column ( | 03-31-2011 |
| 20110077162 | ANALYTICAL MEASURING AND EVALUATION METHOD FOR MOLECULAR INTERACTIONS - The invention relates to an analytical measuring and evaluation method for determining the interaction parameters between an analyte and a ligand, preferably in a biosensor. According to the inventive method, the concentration of the analyte is gradually changed at defined intervals ti and the initial association or dissociation rates or association and dissociation rate constants are determined. The invention further relates to a device for carrying out the inventive method. | 03-31-2011 |
| 20110076703 | CONCENTRATION ASSAY - A method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle comprises contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte. | 03-31-2011 |
| 20110073213 | COLUMN PACKING METHOD - A method for packing a media bed in a column ( | 03-31-2011 |
| 20110070648 | METHOD FOR CELL EXPANSION - The present invention relates to a method for cell expansion. More closely, it relates to a method for expansion of cells, such as mesenchymal stem cells, on microcarriers in a plastic bag bioreactor. The invention enables expansion to therapeutic amounts of stem cells. The method comprises the following steps: a) addition of cells in cell culture medium and microcarriers to a plastic bag container; b) allowing the cells to adhere to the microcarriers while the container is kept substantially still; c) addition of further cell culture medium once the cells have adhered; d) culturing the cells under gentle and constant agitation; e) increase the surface area for continued culturing; and f) final harvesting of cells by an active detachment and separation step. | 03-24-2011 |
| 20110053225 | MULTIFUNCTIONAL TAGS - The present invention relates to multifunctional protein production tags comprising at least 4, preferably 4-8 amino acids, for optimising expression of proteins and purification thereof by various multi-step processes including chromatographic or filtration, as well as batch unit operations. The tags include sequences of multiple defined purposes which are not generated in defined linear sequence regions related to one purpose but as integrated sequences often overlapping each other, i.e. the defined purposes are not discrete separate units on the tag but rather heterogeneously distributed in the tag. The invention also relates to an expression vector encoding the multipurpose tag and a method for protein purification, comprising expressing a protein in the expression vector and purifying the protein in several steps using functionalities of the multipurpose tag. | 03-03-2011 |
| 20110053127 | COLUMN PACKING METHOD - A method for packing a media bed in a column ( | 03-03-2011 |
| 20110042290 | CHROMATOGRAPHY COLUMN AND MAINTENANCE METHOD - A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device. | 02-24-2011 |
| 20110039712 | PREPARATION OF LIQUID MIXTURES - A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device. | 02-17-2011 |
| 20110003941 | METHOD FOR PREPARATION OF A BIOMOLECULE ADSORBENT - The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: | 01-06-2011 |
| 20100332174 | METHOD AND APPARATUS FOR QUANTITATING SURFACE-BINDING OPTICAL RESONANCE PROFILES - Empirical Profile Fits are used to quantitate Surface-binding Optical Resonance profiles. The EPF process has two stages, Calibration and Fit. In the Calibration stage, a calibration surface-binding optical resonance scan is obtained with relatively fine angle or wavelength spacing over a range sufficient to include full resonance profiles for all regions. Smoothed, subsampled empirical profiles for each Region of Interest are generated by the main calibration module, together with first derivative curves and diagnostic information. Properties returned may include approximate resonance position, depth, and width. In the Fit stage, individual ROI scans are used for measurement of resonance shifts relative to the empirical profile. The fitting module identifies the region of the experimental scan encompassing the resonance and fits that region using the previously stored empirical profiles, quantifying and returning the desired values, including the shift in the resonance as compared to its location at calibration, estimated absolute angles or wavelengths, time of resonance minimum, and additional diagnostic and quality information. Optionally, data obtained from either Calibration or Fit stages may be exported for analysis on other systems. In a preferred embodiment, Instrument Control and Data Acquisition Software sets internal parameters in the EPF Calibration module and sends raw data from a calibration scan to the EPF Calibration module, which funnels the data through a Subsampler and a Savitsky-Golay smoothing routine before taking derivatives and characterizing the data to create an empirical profile for the chip. The empirical profiles are then optionally stored. Next, Instrument Control and Data Acquisition Software sets internal parameters in the EPF Fitting module and sends raw data from a run-time scan performed utilizing the chip to the EPF Fitting module, which qualifies it, queries the EPF Calibration module for the empirical profile for the chip, and fits the curve, iterating when necessary. Results from the fitting process are then returned and thereby provided to the user. | 12-30-2010 |
| 20100320149 | SEPARATION MATRIX - The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used. | 12-23-2010 |
| 20100298548 | METHOD FOR PRODUCTION OF SEPARATION MEDIA - The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies. | 11-25-2010 |
| 20100294977 | SEPARATION OF BIOMOLECULES - The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules. | 11-25-2010 |
| 20100282610 | METHOD FOR SAMPLE APPLICATION - The present invention relates to a method for sample application and separation. More closely, the invention relates to convenient direct loading of a biomolecule sample via magnetic beads to, for example, a gel before electrophoresis. In this way, the invention combines elution and application steps with minimal losses of sample. Thus, the invention relates to a method for sample application of biomolecules on a separation media, comprising the following steps: a) obtaining said biomolecules from a sample by magnetic beads; b) applying the magnetic beads with the biomolecules to a separation medium; c) releasing the biomolecules into the separation media, and d) separation of the biomolecules from each other in the separation medium. | 11-11-2010 |
| 20100276371 | CHROMATOGRAPHY COLUMN AND METHOD OF MAINTENANCE - A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device. | 11-04-2010 |
| 20100276370 | METHOD FOR CONDUCTING MAINTENANCE ON A CHROMATOGRAPHY COLUMN - The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components. | 11-04-2010 |
| 20100273384 | METHOD FOR ALIGNING A LAMP ARC IN AN OPTICAL DEVICE - In a method for aligning the arc of a lamp with the optical axle of an optical device the lamp is inserted in a housing of a solid material, said housing having an outer configuration unambiguously adapted to a holder of the unit. The lamp is thereafter adjusted to its correct position with regard to said optical axle and fixed in said position. | 10-28-2010 |
| 20100273240 | METHOD FOR PRODUCTION AND PURIFICATION OF MACROMOLECULAR COMPLEXES - The present invention relates to a method for production and purification of affinity tagged macromolecular complexes, such as ribosomes. More closely, the method comprises in-frame fusion of a nucleotide sequence specific for an affinity tag and a selection marker, wherein the fusion is at the chromosomal site of a gene encoding a multicopy protein, and wherein the macromolecular complex is expressed with multiple copies of said affinity tag. The invention also relates to affinity tagged ribosomes, to cells comprising such affinity tagged ribosomes, and to various uses thereof. | 10-28-2010 |
| 20100272614 | CASSETTE FOR TEST TUBES - In a cassette for test tubes for use e.g. in a fraction collector the test tubes are kept in a fixed position by means of holding means arranged on an adjustable hold and release plate, which, when moved to its release position, releases said test tubes. | 10-28-2010 |
| 20100260642 | METHOD AND DEVICE FOR LAMINAR FLOW ON A SENSING SURFACE - Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided. | 10-14-2010 |
| 20100252491 | MULTI-COMPONENT PART TRANSDUCER ASSEMBLY AND A METHOD FOR DETERMINING THE PRESSURE OF A FLUID USING THE TRANSDUCER - The invention relates to a multi-component part transducer assembly for determining the pressure of a fluid of interest. One part of the assembly comprises a reusable transducer, another part a disposable component comprising a flexible membrane which is in pressure connection with the fluid of interest, and another part comprises a locking component for securing the reusable and disposable parts together. | 10-07-2010 |
| 20100230340 | SEALING ASSEMBLY FOR A CHROMATOGRAPHY COLUMN - The present invention relates to a sealing assembly for use in chromatography columns and methods for forming a seal in such columns. The assembly comprises a sealing element composed of a material having a low coefficient of friction and a separate resilient member co-operating therewith. The seal provided is stable over a temperature range of +2° C. to +30° C. | 09-16-2010 |
| 20100213065 | METHOD AND DEVICES FOR FORMING A PLURALITY OF WELLS ON A GEL - The present invention relates to methods and devices for forming a plurality of wells on a gel containing an analyte. The invention further provides a system for eluting a liquid analyte reagent mixture from a gel. The invention is useful in the separation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for separating and eluting peptides from isoelectric focusing gels. | 08-26-2010 |
| 20100181241 | CHROMATOGRAPHY COLUMN AND METHOD OF MAINTENANCE - A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device. | 07-22-2010 |
| 20100181197 | ISOELECTRIC POINT MARKERS - The present invention relates to the field of isoelectric focussing or 2D electrophoresis, in particular isoelectric point markers with fluorescence detection. The marker comprises an oligopeptide covalently labelled with a fluorescent dye and is characterised in that the dye has a net charge that will maintain the overall net charge of the oligopeptide upon being bound to the oligopeptide. The invention also relates to an electrophoretic method for analysis of proteins using these markers. | 07-22-2010 |
| 20100179252 | POLYMER TWO PHASE SYSTEM AND USE THEREOF - The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles. | 07-15-2010 |
| 20100176058 | CHROMATOGRAPHY METHOD - The present invention relates to a simulated moving bed process, wherein at least one adsorbent is washed after binding of target compound and wherein the outlet of wash liquid from the adsorbent is subsequently passed onto another adsorbent for binding of target compound removed by the washing. In one embodiment, the method comprises binding of at least one target compound using three or more adsorbents connected in series and elution of target compound from said three adsorbents. After the binding to an adsorbent, wash liquid is passed across the adsorbent to recover desorbed and/or unbound target compound, and the outlet of such wash liquid is directed to the adsorbent after the next in the series, to which no feed has yet been added. The target compound is recovered by eluting target compound from the washed adsorbents. | 07-15-2010 |
| 20100174052 | SEPARATION METHOD USING POLYMER MULTI PHASE SYSTEMS - The present invention relates to a process of isolating one or more target compounds, wherein the clarification of feed is performed using partitioning in a multiphase system comprising a first polymer, which is a synthetic poly(acid), a second synthetic polymer, which is a poly(ether), and at least one salt, which clarification is followed by at least one step of affinity chromatography. The molecular weight of the poly(acid) may be in the range of 1000-100,000 Da. The target compound is preferably a biomolecule, such as a monoclonal antibody. | 07-08-2010 |
| 20100163416 | ELECTRODIC BRIDGE - The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilised pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips. | 07-01-2010 |
| 20100151581 | PURIFICATION OF IMMUNOGLOBULINS - The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands. | 06-17-2010 |
| 20100143895 | METHODS AND SYSTEMS FOR ADDING A REAGENT TO AN ANALYTE IN A GEL - The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels. | 06-10-2010 |
| 20100136647 | METHOD FOR PRODUCTION OF CELL ATTACHMENT AND CULTURE SURFACES - The present invention relates to the field of adherent cell culture. More closely, the invention relates to a method for production of a cell attachment and culture surface, such as a microcarrier, comprising a guanidino-containing ligand, wherein the ligand is coupled via reaction involving a primary amine to the surface which is activated by activation groups such that the final molar ratio of grafted ligand and ungrafted activation groups is above 1.5. Preferably, the ligand density is above 0.5 mmol/g cell culture surface and the remaining activation groups after coupling is less than 0.6 mmol/g cell culture surface. The cell culture surface may be used for various purposes, primarily cell cultivation and virus production. | 06-03-2010 |
| 20100130354 | NOVEL SEPARATION MATRIX - The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support. | 05-27-2010 |
| 20100121035 | PURIFICATION OF IMMUNOGLOBULINS - The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention. | 05-13-2010 |
| 20100120596 | SEPARATION DEVICE - A separation device adapted for separation of a wanted end product from a sample by centrifugation. According to the invention the device comprises: a first, a second and a third compartment; a first fluid connection between the first and second compartments, arranged to be opened and closed by first closing means; a second fluid connection between the second and the third compartments, arranged to be opened and closed by second closing means. The separation device is adapted to be subjected to centrifugation after having been filled with a first density gradient medium in the first compartment, sample in the third compartment and a second density gradient medium or sample in the second compartment, whereby after centrifugation the wanted end product will settle in the second compartment and the first fluid connection and the second fluid connection will be closed by the first closing means and the second closing means respectively. | 05-13-2010 |
| 20100081121 | MEDIA AND METHOD FOR CELL SEPARATION - The present invention relates to a separation media and a method for separation of cells from different cell sources, such as umbilical cord blood, using the separation media to obtain the desired cells, such as stem cells. The separation media has beads with a diameter of 200-500 μm and is provided with cell separation ligands. The cell specific ligands are preferably CD3 and CD19 for depletion of B and T cells and production of a stem cell rich product. | 04-01-2010 |
| 20100069617 | ENHANCED PROTEIN AGGREGATE REMOVAL BY MIXED MODE CHROMATOGRAPHY ON HYDROPHOBIC INTERACTION MEDIA IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - This invention relates to methods for enhancing purification of proteins such as monoclonal antibodies by chromatography on carboxyl group-containing HIC supports in the presence of zwitterions that are excluded from protein surfaces. In certain embodiments, the invention may permit more effective separation of non-aggregated protein from aggregated protein. | 03-18-2010 |
| 20100058841 | FLOW DISTRIBUTING VALVE - A rotary valve ( | 03-11-2010 |
| 20100040507 | FIXTURES FOR USE IN PARALLEL PROCESSING BIO-CHIPS | 02-18-2010 |
| 20100035273 | METHOD AND DEVICE FOR SMALL SCALE REACTIONS - The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands. | 02-11-2010 |
| 20100032604 | ROTATION VALVE FOR SAMPLE INJECTION - A rotary valve adapted for injection of a fluid sample into a flow path. According to the invention one and the same valve can be used to input flow from a system pump, a sample pump and a syringe. A loop could be filled from both the sample pump and the syringe and the loop can be emptied by the system pump whereby for example a column is filled. Furthermore the sample pump can be used to pump directly to the column. | 02-11-2010 |
| 20100032603 | SELECTION VALVE - A rotary selection valve where different components can be selected in different rotation positions of the valve and furthermore the flow through the components can be reversed by rotating the rotary valve. | 02-11-2010 |
| 20100029015 | METHOD OF COUPLING BINDING AGENTS TO A SUBSTRATE SURFACE - The present invention relates to a method of coupling multiple binding agents to respective areas of a substrate surface by hydrodynamic addressing, using two laminar fluid flows that flow together in the same direction over the substrate surface with an interface to each other to successively couple the binding agents to the substrate areas, wherein each successive coupling of a binding agent to a surface area is followed or preceded by selective deactivation or activation of a selected surface area according to a defined protocol. The invention also relates to the use of such a binding agent-coupled substrate surface for analytical purposes. | 02-04-2010 |
| 20100028505 | LIQUID CLARIFICATION - The present invention relates to liquid clarification. More closely, the invention relates to beverage clarification, such as reduction of colloidal (not microbial) haze-causing substances in beer or related beverages such as wine, juices, flavorings etc. The method of the invention uses a hydrophilic surface for adsorption of haze-forming substances by hydrogen bonding interaction properties between the surface and the haze-forming substances. | 02-04-2010 |
| 20100022760 | MUTANT PROTEIN - The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine. | 01-28-2010 |
| 20100016564 | METHOD OF PREPARING AN IMMOBILISED METAL ION CHROMATOGRAPHY ADSORBENT AND METHODS OF PURIFYING PROTEINS, PEPTIDES OR POLYNUCLEOTIDES - The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A). | 01-21-2010 |
| 20100009867 | MULTI-MODAL ION EXCHANGE CHROMATOGRAPHY RESINS - The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s). | 01-14-2010 |
| 20090281288 | MATRIX FOR SEPARATION OF POLYETHERS AND METHOD OF SEPARATION - The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins. | 11-12-2009 |
| 20090264630 | METHOD OF SEPARATING MONOMERIC PROTEIN(S) - The present invention relates to a method of separating one or more monomeric proteins, such as monomeric antibodies, from a liquid. The method comprises providing a thiophilic aromatic chromatography matrix; contacting the liquid that comprises proteins with the matrix; and recovering at least one monomeric protein, such as a monomeric antibody, from the flow-through fraction. The proteins are advantageously monomeric antibodies. | 10-22-2009 |
| 20090263285 | METHOD AND DEVICE FOR LAMINAR FLOW ON A SENSING SURFACE - Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided. | 10-22-2009 |
| 20090263284 | METHOD AND DEVICE FOR LAMINAR FLOW ON A SENSING SURFACE - Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided. | 10-22-2009 |
| 20090259029 | [1, 2, 4] TRIAZOLO [1, 5-A] PYRIMIDINE DERIVATIVES AS CHROMATOGRAPHIC ADSORBENT FOR THE SELECTIVE ADSORPTION OF IGG - The present invention relates to a chromatographic adsorbent for selectively adsorbing IgG, comprising the following formula | 10-15-2009 |
| 20090253582 | CHAMBER APPARATUS - This invention provides a system that allows a user to assemble a chamber apparatus that prohibits samples from leaking or mixing with other samples or chamber array wells, when they are inserted into an array well of the chamber apparatus. In addition, the chamber frame design allows for easy assembly and disassembly for simplified use and slide substrate scanning on conventional microarray scanners. Chamber apparatus includes a chamber frame with an upper integrated gasket and a lower integrated gasket, a substrate, and a substrate frame that positions and captures the substrate. The lower integrated gasket provides a single sealing surface between the chamber frame and the substrate. The upper integrated gasket interfaces with a chamber cover forming a compression seal that prevents sample loss due to evaporation during the hybridization process. The chamber frame and substrate frame have integrated features that allow them to align and fasten to each other by latching or snapping resulting in an optimal clamping force to produce a compression seal between the integrated lower gasket and the substrate. | 10-08-2009 |
| 20090242750 | PROTEIN PURIFICATION AND IDENTIFICATION - The present invention relates to a method for protein purification and identification. More closely the invention relates to a method for protein pre-fractionation and identification resulting in improved yield of identified proteins. The method for pre-fractionation of protein samples, includes the following steps: a) reducing disulphide bridges (S-S bridges) or protecting cysteines in the proteins in the sample; b) loading the sample onto an ion exchange column; c) eluting the sample; d) collecting each fraction from the column separately in air sealed containers devoid of chromatographic media; e) desalting each fraction on a single RPC (reversed phase chromatography) trap column; f) separating each fraction in a second dimension RPC step to obtain further separated proteins which are collected in fractions; and g) identifying the further separated proteins by MS. | 10-01-2009 |
| 20090213382 | OPTICAL RESONANCE ANALYSIS UNIT - An array surface plasmon resonance (SPR) analysis instrument comprising a reflective SPR sensor array, a light source assembly arranged to project a collimated beam of light onto the reflective SPR sensor array to provide a reflected array image of the sensor array, and to scan the incident angle of the collimated beam of light over an angular range, and a detector assembly oriented to receive the reflected array image of the sensor array over the angular range, the detector assembly comprises a two-dimensional detector sensing element that is tilted with respect to the optical axis of the lens assembly in accordance with the Scheimpflug condition, and a lens assembly for focusing the reflected array image of said SPR sensor array onto said tilted detector sensing element. The lens assembly comprises an objective section which is arranged to produce a virtual image of the tilted reflected array at infinity, followed by an imaging section arranged to transform the virtual image of the tilted reflected array into a real tilted image on the tilted detector sensing element. | 08-27-2009 |
| 20090209656 | DISINFECTION/SANITATION METHOD WHERE THE MATERIAL IS TREATED WITH A SOLUTION COMPRISING BENZYL ALCOHOL AND C2-3 ALCOHOL - The present invention relates to a method for disinfection or sanitation of different material, especially sensitive material, such as chromatographic media with various sensitive ligands. The invention also relates to a composition for use in such a method. The method for disinfection/sanitation, comprises exposing the material to be treated to a solution comprising 5-10% benzyl alcohol and 10-30% C | 08-20-2009 |
| 20090209402 | CENTRIFUGAL SEPARATION SYSTEM - The invention relates to a system, and a set ( | 08-20-2009 |
| 20090200239 | METHOD FOR GENERATING METAL CHELATING AFFINITY LIGANDS - The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of | 08-13-2009 |
| 20090188853 | CHROMATOGRAPHY COLUMN DISTRIBUTION SYSTEM - A chromatography column distribution system ( | 07-30-2009 |
| 20090186366 | METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS - A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light. | 07-23-2009 |
| 20090184052 | METHOD FOR REMOVING AIR FROM A CHROMATOGRAPHY COLUMN - The invention relates to a method for removing air from the bed space of a chromatography column. The method involves use a chromatography column having an inner wall which comprises at least one elongated groove through which air can pass from the bed space to a chamber above an adapter assembly. | 07-23-2009 |
| 20090166290 | AUTOMATED LOW VOLUME CROSSFLOW FILTRATION - The invention provides an automated crossflow filtration method and system for separating a component of interest from one or more other components in a solution. The invention is of particular use in the field of protein separations and concentration, where specific proteins must be separated and purified from cell lysates and cultures. The system may be under the control of a computer software programme. | 07-02-2009 |
| 20090138208 | IMMUNOGLOBULIN G BINDING POCKET - The present invention relates to a human IgG binding pocket comprised of a first interacting surface, which originates from an IgG κ light chain, and a second interacting surface, which originates from an IgG heavy chain, which amino acids are strictly conserved between human IgGs of κ-type. The invention also embraces an isolated and purified polypeptide, which comprises said binding pocket. Further, the invention relates to various methods of using the novel binding pocket, such as in screening for identification of chemical entities capable of selective binding thereof, and in other experimental and/or virtual methods for design and/or identification of chemical entities capable of selective binding thereof. | 05-28-2009 |
| 20090127115 | Method and Devices For Imaging a Sample - The invention relates to methods and devices for imaging a sample, in particular to methods and devices for imaging electrophoretic gels which have been used to separate biological molecules such as proteins or nucleic acids. The invention overcomes the problems associated with interference due to Newton's Rings and chemical toxicity experienced with conventional imaging systems. | 05-21-2009 |
| 20090095668 | PREPARATION OF MONOLITHIC ARTICLES - The present invention relates to a method of producing a polymeric monolithic article by radical polymerization, which method comprises providing a mold comprising a solution of radically polymerisable monomers; a transition metal catalyst and a complexing ligand in a solvent; adding an ATRP initiator and, optionally, flushing the mixture with an inert gas; carrying out radical polymerisation in the mold; optionally, removing the monolithic article obtained in from the mold; and washing the monolithic article obtained as described above. | 04-16-2009 |
| 20090092837 | MAGNETIC BEADS - The present invention relates to magnetic beads in the form of composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and thereafter a hydrophilic porous polymer, preferably dextran. This provides porous biocompatible beads without metal leakage. This construction also allows for simple and convenient handling of cell expansion media by magnetism. | 04-09-2009 |
| 20090053817 | FIXED CHARGE REAGENTS - The present invention relates to fixed charge reagents and kits for use in tandem mass spectrometry methods involving multiplex analysis. The compounds of the invention are phenacylamide compounds. The invention also relates to methods for the quantification of for example peptides and proteins by tandem mass spectrometry techniques using said reagents and kits. The reagents and kits of the invention enable multiplexed analysis of several samples in one experiment. | 02-26-2009 |
| 20090048456 | ACTIVATED SOLID SUPPORT AND METHOD - Disclosed is a method for activating a solid support material with epoxy groups and for immobilising ligands thereon, utilising phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilising a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography. | 02-19-2009 |
| 20090047734 | METHOD OF SEPARATION OF DEOXYRIBONUCLEIC ACIDS - The present invention relates to a method of separating nucleic acids from a liquid, which comprises providing a mobile phase comprising nucleic acids and a first salt, which forms lyotropic ions when dissolved; passing said mobile phase across a hydrophobic interaction chromatography (HIC) matrix to adsorb deoxyribonucleic acid(s); passing an eluent across said matrix to desorb one or more deoxyribonucleic acid(s), which eluent comprises the first salt as well as an increasing gradient provided by a second salt, which second salt forms less lyotropic ions when dissolved than the first salt; and isolating at least one fraction comprising separated deoxyribonucleic acid(s). | 02-19-2009 |
| 20090044393 | CHROMATOGRAPHY COLUMN AND METHOD OF MAINTENANCE - A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device. | 02-19-2009 |
| 20090039023 | CHROMATOGRAPHY COLUMN - A chromatography column having a longitudinal axis and comprising a column wall with a first end and a second end, a first end plate assembly removably connectable to said first end of the column wall, a second end plate assembly removably connectable to said second end of the column wall, wherein said first end plate assembly, said column wall and said second end plate assembly are arranged along the longitudinal axis of the column wherein the column wall, and/or first end plate assembly and/or second end plate assembly is/are rotatable about an axis of rotation wherein said axis of rotation is parallel to the longitudinal axis of said column and positioned outside the column. | 02-12-2009 |
| 20090039008 | METHOD FOR CONDUCTING MAINTENANCE ON A CHROMATOGRAPHY COLUMN - The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components. | 02-12-2009 |
| 20090026081 | METHOD, COMPOSITION AND KIT FOR ISOELECTRIC FOCUSING - The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra. | 01-29-2009 |
| 20090007643 | DETERMINATION OF SLURRY CONCENTRATION - A method for determining the concentration of a chromatographic medium in a liquid slurry comprises the steps of: providing a column ( | 01-08-2009 |
| 20080318327 | Kit and Method for Mass Labelling - The present invention relates to a kit for mass labelling of peptides in two or more samples, comprising a set of mass tagging reagents for reaction at the N-terminals of the peptides and a set of mass balancing reagents for reaction at the C-terminals of the peptides, or vice versa, and wherein each mass tagging reagent in the set is matched with mass balancing reagent(s) in such a way that the sum of the masses from all matched mass tagging and mass balancing reagents is equal. The invention also relates to a method of using said mass labels and a database for use in said method. | 12-25-2008 |
| 20080314750 | Method for Pre-Fractionation of Complex Samples - The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps:
| 12-25-2008 |
| 20080308498 | CHROMATOGRAPHY COLUMN AND METHOD OF MAINTENANCE - A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device. | 12-18-2008 |
| 20080304048 | Methods and Apparatus For Measuring the Concentration of a Substance in a Solution - The present invention relates to methods and apparatus for detecting and measuring the concentration of a substance in a solution, the substance having an absorption at 300 nm or less. The methods and apparatus have particular utility in detecting and measuring the concentration of proteins and nucleic acids. | 12-11-2008 |
| 20080299671 | Hydrophobic Interaction Chromatography - The present invention relates to a method of isolating target compounds from a liquid, which method including the steps of providing a mobile phase, which contains at least one target compound and wherein the conductivity corresponds to ≧0.6 M; contacting the mobile phase with a separation matrix including one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with the matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid. | 12-04-2008 |
| 20080293926 | Preparation of Biomolecules - The present invention relates to a method of separating a target from a liquid, which includes providing a polymer in an aqueous liquid, which polymer comprises at least one hydrophobic portion; contacting the polymer-containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained. One phase is polymer-rich and contains target(s) and another phase is polymer-poor. By either maintaining the stimulus, or applying a different stimulus and maintaining it, the polymer-rich phase is transformed into a substantially solid phase. | 11-27-2008 |
| 20080293083 | Method and Kit for Peptide Analysis - The present invention relates to a method for peptide analysis, comprising the following steps: a) tagging N-terminals of peptides in sample(s) with mass tagging reagent(s) and mass balancing C-terminals of said peptides with mass balancing reagent(s), or vice versa; and b) mass spectrometry analysis of said peptides. The present invention also relates to a kit with global mass tagging reagents and mass balancing reagents for use in said method and a database with specific peptide information. | 11-27-2008 |
| 20080289963 | Gel for Isoelectric Focusing - The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel. | 11-27-2008 |
| 20080283792 | Separation Medium with Various Functionalities - The present invention relates to a separation medium with various functionalities suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The separation medium comprises magnetic metal particles, preferably coated with an inert synthetic polymer, and pre-functionalised beads. These particles and beads are provided encapsulated in a hydrophilic porous polymer, preferably agarose. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer may be provided with ligands having affinity for selected biomolecules. | 11-20-2008 |
| 20080272045 | AUTOMATED PACKING SYSTEM AND METHOD FOR CHROMATOGRAPHY COLUMNS - A column packing system comprises a control unit provided with software for calculating the breakpoint where a movable adapter comes into contact with a settled bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression. | 11-06-2008 |
| 20080260594 | Multiwell Plate - Multiwell plate ( | 10-23-2008 |
| 20080249289 | Manufacture of a Chromatography Matrix - The present invention relates to method of manufacturing a sulphonate-functionalized (S-functionalized) cation exchanger, which method comprises reacting olefinic groups with bisulphite in the presence of at least one amine oxide, such as N-methyl morpholine oxide (NMO). The olefinic groups of the carrier may be allyl groups; which may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties. | 10-09-2008 |
| 20080237124 | Manufacture of Chromatography Matrices - The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties. | 10-02-2008 |
| 20080230478 | Regeneration Of A Chromatography Matrix - The present invention relates to a process of regenerating a separation matrix, such as a chromatography matrix, comprising adsorption of at least one target molecule by contacting a mobile phase comprising at target molecule(s) with a matrix; removal of unbound material by washing the matrix; elution of target molecule(s) by contacting the matrix with an eluent; reducing regeneration by contacting said matrix with a reducing agent; alkaline regeneration by contacting the matrix with an alkaline solution; and equilibration of the matrix. | 09-25-2008 |
| 20080227206 | METHOD AND SYSTEM FOR DETERMINATION OF MOLECULAR INTERACTION PARAMETERS - A method of determining kinetic parameters for a reversible molecular interaction between a ligand immobilized to a solid support surface and a binding partner to the ligand in solution, comprises sequentially, without intermediate regeneration or renewal of the immobilized ligand, flowing a plurality of fluid volumes containing different known concentrations of the binding partner over the solid support surface, monitoring the momentary amount of binding partner bound to the solid support surface related to time and solution concentration of binding partner and collecting the binding data, and determining the kinetic parameters by globally fitting a predetermined kinetic model for the interaction between the binding partner and the immobilized ligand to the collected binding data, which model allows for mass transport limitation at the solid support surface. An analytical system for carrying out the method, a computer program, a computer program product and a computer system for performing the method are also disclosed. | 09-18-2008 |
| 20080220968 | [1, 2, 4] Triazolo [1, 5-A] Pyrimidine Derivatives as Chromatographic Adsorbent for the Selective Adsorption of Igg - The present invention relates to a chromatographic adsorbent for selectively adsorbing IgG, comprising the following formula | 09-11-2008 |
| 20080217248 | PACKING SYSTEM AND METHOD FOR CHROMATOGRAPHY COLUMNS - The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns which utilises an external drive means to compress a bed of particulate medium to a target bed height. | 09-11-2008 |
| 20080212073 | POLYMERIC DEVICE SUITABLE FOR ULTRAVIOLET DETECTION - The present invention relates to a flow cell ( | 09-04-2008 |
| 20080199959 | Method For Cell Culture - The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion. | 08-21-2008 |
| 20080196511 | ULTRASONIC FLOW METER - The invention relates to a flow cell for an ultrasonic flow meter comprising two transducers of ultrasonic waves wherein the two transducers are reversibly detachable from the flow cell. The flow cell with the transducers detached is sterilisable and is particularly suitable for use in disposable or single-use systems. | 08-21-2008 |
