20120040391 | Sepsis diagnostic test - The invention relates to a method for the extracorporeal qualitative or semi-quantitative determination of the amount of indicators for the systemic inflammatory response system (SIRS) or sepsis in the blood, blood serum, blood plasma, other body fluids or lavages or their constituents of human or animal subjects. In order to provide a test which is better than that of the prior art, with which the presence and/or the severity of SIRS or sepsis can be rapidly, economically, reliably and reproducibly determined in a sample, such as blood serum of a patient. To this end, the inventive method has the following steps in which: a cell line is prepared in a culture; in at least one first preparation, cells of the cell line are brought into contact with blood, blood serum or blood plasma, other body fluids or lavages or their constituents of a human or animal subject to be examined, and into contact with a reagent that reacts to reactive oxygen intermediates (ROI), and enters into a color, light or other measurable reaction; in at least one other preparation, cells of the cell line are brought into contact with blood, blood serum or blood plasma, other body fluids or lavages or their constituents of a human or animal control subject that is not ill with SIRS or sepsis, and into contact with a reagent that reacts to reactive oxygen intermediates (ROI), and enters into a color, light or other measurable reaction; the color, light or other measurable reactions are measured in the preparations, and; the measured values of the subject to be examined are compared with those of the control subject. | 02-16-2012 |