Brandeis University Patent applications |
Patent application number | Title | Published |
20150259391 | TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS - Nonsense-mediated mRNA decay (NMD) polypeptides, nucleic acids encoding NMD polypeptides, and methods of using such polypeptides and nucleic acids in the treatment of ALS and in screening for agents for the treatment of ALS are described. | 09-17-2015 |
20150248002 | COOLING SYSTEMS AND METHODS FOR CRYO SUPER-RESOLUTION FLOURESCENCE LIGHT MICROSCOPY AND OTHER APPLICATIONS - New systems and methods are described for maintaining a desired steady state temperature differential between two objects that may otherwise undergo heat transfer to restore thermal steady state. In one application, a cooling microscope assembly and its use with conventional optical microscopes are described for achieving super-resolution imaging. The assembly allows for the high resolution imaging of samples at cryogenic temperatures while maintaining the temperature of the objective lens above freezing by employing circulation systems and a coupling fluid between the sample and objective lens. | 09-03-2015 |
20150218625 | COMBINATION OF DSDNA BINDING DYE AND PROBES FOR CHARACTERIZATION OF SSDNA SEQUENCES - This invention includes methods for analyzing single-stranded nucleic acid sequences, either RNA sequences or DNA sequences (ssDNA) utilizing dyes that fluoresce when associated with double strands, so-called DNA binding dyes or dsDNA-dyes. Methods according to this invention utilize a dsDNA-dye in combination with one or more hybridization probes that hybridize to a target nucleic acid sequence and that are labeled with a non-fluorescent quencher moiety, for example, a Black Hole quencher. | 08-06-2015 |
20140315211 | METHODS FOR SUPPRESSION PCR - Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5′-3′ exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction. | 10-23-2014 |
20140302303 | ALIGNED ARRAYS OF NANORODS, AND METHODS OF MAKING AND USING THEM - Described are methods for the assembly of monolayer, bilayer, or multi-layer structures composed of homogenous rod-like molecules and particles. Included are methods for tuning physical properties of the mono-or multi-layered structures by changing ionic conditions and the size or concentration of polymer used for self-assembly. | 10-09-2014 |
20140295572 | MANIPULATION OF FLUIDS AND REACTIONS IN MICROFLUIDIC SYSTEMS - Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions. | 10-02-2014 |
20140288145 | TREATMENT OF AMYLOIDOSIS BY COMPOUNDS THAT REGULATE RETROMER STABILIZATION - The present invention provides pharmaceutical compositions and related methods for stabilizing retromer in cells. | 09-25-2014 |
20140249313 | Conjugate Addition Reactions Using Bifunctional Cinchona-Alkaloid-Based Catalysts - One aspect of the present invention relates to quinine-based and quinidine-based catalysts. Another aspect of the present invention relates to a method of preparing a chiral, non-racemic compound from a prochiral electron-deficient alkene, comprising the step of: reacting a prochiral electron-deficient alkene with a nucleophile in the presence of a catalyst; thereby producing a chiral, non-racemic compound; wherein said catalyst is a derivatized quinine or quinidine. | 09-04-2014 |
20140246098 | MANIPULATION OF FLUIDS, FLUID COMPONENTS AND REACTIONS IN MICROFLUIDIC SYSTEMS - Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply. | 09-04-2014 |
20140235550 | Antineoplastic Hydrogels, and Enzyme-Instructed Preparations Thereof - Disclosed is a general methodology to create nanofibers of therapeutic molecules that have a dual role, as both the delivery vehicle and the drug itself. It is shown that with proper molecular design, the integration of enzymatic reaction and self-assembly provides a powerful method to create molecular hydrogels of clinically-used therapeutics without compromising their bioactivities. In addition, the results disclosed herein demonstrate enzyme-instructed self-assembly as a facile strategy for generating the supramolecular hydrogels of molecules that inherently have poor solubility in water. For example, by covalently connecting paclitaxel with a motif that is prone to self-assemble, a hydrogel of paclitaxel can be formed without compromising the activity of the paclitaxel. | 08-21-2014 |
20140206564 | REAGENTS FOR IMPROVING PCR ACCURACY - Provided herein are reagents for improving PCR accuracy. | 07-24-2014 |
20140148410 | Supramolecular Nanofibers and Hydrogels Based on Nucleic Acids Functionalized with Nucleobases - Disclosed are nucleopeptide compounds that include a nucleobase, and an amino acid. Certain compounds further comprise a glycoside. The compounds may self-assemble to form supramolecular hydrogels. Also, the compounds may be used as a platform to examine specific biological functions (e.g., binding to DNA and RNA) of a dynamic supramolecular system that is able to interact with both proteins and nucleic acids. Other uses include: methods of growing cells and methods of delivering a substance to a cell. | 05-29-2014 |
20140147846 | DETECTION OF SEQUENCE VARIANTS IN THE HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) GENE - Provided herein are methods for detecting and identifying sequence variants in the human epidermal growth factor receptor (EGFR) gene, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided for the detection and identification of EGFR sequence variants. | 05-29-2014 |
20140141419 | SINGLE TUBE QUANTITATIVE POLYMERASE CHAIN REACTION (PCR) - Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (PCR). In particular, quantitative monitoring of target amplicon(s) in a single-tube PCR reaction without separate calibration reactions are provided. | 05-22-2014 |
20140127386 | VEGETABLE OIL COMPOSITION CONTAINING PALM MID-FRACTION FAT AND METHOD OF REDUCING PLASMA CHOLESTEROL - A method and composition for reducing the cholesterolemic effect in mammals of ingesting a blended nutritional fat composition containing a palm mid-fraction (PMF) hardstock fat combined with an unsaturated vegetable oil. The composition is solid or semi-solid at 20° C. and fluid at 35° C., and includes between 15% and 45% by weight linoleic acid. The weight ratio of disaturated triglyceride (DST) molecules to trisaturated triglyceride (TST) molecules is greater than 10:1, and the PMF hardstock fat contains approximately 50% to 95% by weight DST molecules, the majority of which contain either palmitic acid or a combination of palmitic and stearic acids at the sn-1 and sn-3 triglyceride positions and either oleic acid or linoleic acid at the sn-2 molecular position. | 05-08-2014 |
20140127349 | VEGETABLE OIL COMPOSITION CONTAINING PALM MID-FRACTION FAT AND METHOD OF REDUCING PLASMA CHOLESTEROL - A method and composition for reducing the cholesterolemic effect in mammals of ingesting a blended nutritional fat composition containing a palm mid-fraction (PMF) hardstock fat combined with an unsaturated vegetable oil. The composition is solid or semi-solid at 20° C. and fluid at 35° C., and includes between 15% and 45% by weight linoleic acid. The weight ratio of disaturated triglyceride (DST) molecules to trisaturated triglyceride (TST) molecules is greater than 10:1, and the PMF hardstock fat contains approximately 50% to 95% by weight DST molecules, the majority of which contain either palmitic acid or a combination of palmitic and stearic acids at the sn-1 and sn-3 triglyceride positions and either oleic acid or linoleic acid at the sn-2 molecular position. | 05-08-2014 |
20140024033 | DETECTING NUCLEIC ACID VARIATIONS WITHIN POPULATIONS OF GENOMES - This disclosure relates to amplification and detection of a rare variant or variants of a DNA sequence in an abundant variant of the sequence, such as detection of a low-level somatic mutations and minority alleles in an excess of normal nucleic acid target sequences. | 01-23-2014 |
20140004513 | COMPOSITIONS, METHODS, AND KITS FOR DETECTING AND IDENTIFYING MYCOBACTERIA | 01-02-2014 |
20140004504 | COMPOSITIONS AND METHODS FOR THE DETECTION AND ANALYSIS OF AFRICAN SWINE FEVER VIRUS | 01-02-2014 |
20130344484 | DETECTING MUTATIONS IN DNA - Provided herein are methods for detecting mutations in nucleic acid, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided to detect mutations and assess the mutational load. The methods are based on a set of adjacently binding probes wherein one probe is labelled with a quencher and the other is a self-indicating probe labelled with fluorophore and quencher. The binding of the probes is analysed by melting curve analysis. | 12-26-2013 |
20130309690 | TETRAMERIC ALPHA-SYNUCLEIN AS BIOMARKERS - The present invention provides the surprising finding that alpha-synuclein exists in vivo as a folded tetramer. Provided are various methods and technologies that arise from this finding, including methods and kits for identifying individuals susceptible to or suffering from certain diseases, disorders or conditions associated with stability of alpha-synuclein tetramers, and/or individuals likely (or not) to respond to therapy with agents that alter level and/or stability of alpha-synuclein tetramers. | 11-21-2013 |
20130295570 | COMPOSITIONS AND METHODS FOR NUCLEIC ACID BASED DIAGNOSTIC ASSAYS - The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides probes and non-amplifiable controls for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides probe design criteria for probes for use in amplification/detection assays. Further embodiments of the present invention provide non-amplifiable controls for use in generating reference probe signal ratios in amplification detection assays. | 11-07-2013 |
20130289022 | TETRAMERIC ALPHA-SYNUCLEIN AND USE THEREOF - Disclosed herein are methods and related compositions for identifying compounds that stabilize natively folded tetrameric α-synuclein. These methods and compositions are useful for the treatment and diagnosis of α-synuclein-associated diseases and disorders. | 10-31-2013 |
20130224305 | METHODS OF IDENTIFYING INSECT-TRPA1 MODULATORS - The invention provides a screening method for identifying an insect-specific TRPA1 modulator by comparing modulation of an insect TRPA1 and a mammalian TRPA1. The invention further provides method of insect control by applying to an insect a insect-specific TRPA1 modulator identified by the screening method. | 08-29-2013 |
20130210890 | CANCER THERAPY - The present invention provides agents useful in the treatment of cancer, as well as systems for identifying and/or characterizing such agents, and systems for identifying and/or characterizing patient populations responsive to particular agents. | 08-15-2013 |
20130210656 | LATE-PCR - A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays. | 08-15-2013 |
20130203626 | COMPOSITIONS AND METHODS FOR NUCLEIC ACID BASED DIAGNOSTIC ASSAYS FOR VARIABLE SEQUENCE TARGETS - The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides primers for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides multiple primers for amplification of related nucleic acid targets in a single reaction. | 08-08-2013 |
20130190340 | Small-Molecule-Targeted Protein Degradation - Certain aspects of the invention relate to compounds, compositions and methods that are useful for treating or preventing a disease in a subject by enhancing the degradation of a protein. In other aspects, said compounds can be useful research tools for investigating protein degradation. In other aspects, said compounds are useful research tools for investigating protein function. In certain embodiments, the degraded protein is implicated in a disease or disorder whose pathology is related at least in part to the excessive expression of the protein or the expression of a mutant form of the protein. | 07-25-2013 |
20130141077 | CURRENT METER - Apparatus, usable to measure current in a multiple-conductor cable having supply and return conductors spaced apart by a nominal conductor spacing and carrying respective supply and return currents in opposite directions, includes (1) a non-magnetic body having a cable-engaging portion defining a location and an orientation axis of the multiple-conductor cable relative to the apparatus when the cable-engaging portion engages the cable during use, (2) a planar, multi-turn wire coil supported by the body immediately adjacent to the location and lying in a plane parallel to the orientation axis, the wire coil having a coil diameter at least four times the nominal conductor spacing, and (3) signal conditioning circuitry operative in response to a voltage signal developed across output ends of the wire coil to generate a conditioned voltage signal having a voltage magnitude determined by and indicative of a magnitude of the supply current during use. | 06-06-2013 |
20130116417 | Methods for the Development of Vaccines Based on Oligosaccharide-Oligonucleotide Conjugates - Described herein are oligosaccharide-oligonucleotide conjugates useful as vaccines against one or more human or veterinary therapeutic indications, and methods of synthesizing and identifying them. The conjugates may be identified using non-human antibodies as binding targets, thereby expanding the power and scope of the invention. Efficacious conjugates may be identified through an iterative. | 05-09-2013 |
20130095479 | PRIMERS, PROBES AND METHODS FOR NUCLEIC ACID AMPLIFICATION - Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing. | 04-18-2013 |
20130078606 | System And Method For Detecting A Point of Originality In A Writing - This paper proposes a new method for the objective evaluation of student work through the identification of original content in writing assignments. Using WordNet as a lexical reference, this process allows instructors to track how key phrases are employed and evolve over the course of a student's writing, and to automatically visualize the point at which the student's language first demonstrates original thought, phrased in their own, original words. After sketching the method for isolating “points of originality,” the paper provides a method for visualizing the resulting information. By visualizing otherwise subjective information in a way that is objectively intelligible, the goal is to provide educators with the ability to monitor student investment in concepts from the course syllabus, and to extend or modify the boundaries of the syllabus in anticipation of pre-existing knowledge or trends in interest. | 03-28-2013 |
20130053442 | BALANCED MYRISTATE- AND LAURATE-CONTAINING EDIBLE OIL - Nutritional fat or oil-based compositions for increasing HDL cholesterol, decreasing total cholesterol (TC), LDL cholesterol and decreasing the LDL/HDL cholesterol ratio in human plasma are described. The compositions can advantageously include at least 10% of myristic acid and/or lauric acid in which at least 3% of said fatty acid composition is myristic acid and at least 3% is lauric acid; in some cases at least 1% by weight myristic acid and/or lauric acid is esterified at the sn-2 position in triglyceride molecules. The compositions also include between 5% and 40% by weight linoleic acid, and typically further include at least 10% by weight monounsaturated fatty acids and between 15% and 55% by weight total saturated fatty acids. The sum of weight percentages for saturated, monounsaturated and polyunsaturated fatty acids equals 100%. In desirable cases, the composition is substantially cholesterol-free. Also described are fat compositions and diets which are adapted to limit increases in blood triglycerides, total cholesterol, LDL, and VLDL, even in the presence of dietary cholesterol. | 02-28-2013 |
20120282611 | METHODS, KITS AND REACTION MIXTURES FOR ANALYZING SINGLE-STRANDED NUCLEIC ACID SEQUENCES - Provided herein are fluorescence detection methods for nucleic acid sequences and to kits for performing such methods. | 11-08-2012 |
20120282368 | AMPHIPHILIC STEROL/FAT-BASED PARTICLES - The present invention relates to amphiphilic sterol- and fat-based particles, which can be dispersed in liquid foods and beverages such as milk. The compositions provided, when consumed, reduce low-density lipoprotein (LDL) and total plasma cholesterol (TC) levels. | 11-08-2012 |
20120264760 | Compounds and Methods for Treating Mammalian Gastrointestinal Parasitic Infections - One aspect of the present invention relates to compounds, and pharmaceutically acceptable salts and prodrugs thereof, that are useful as inhibitors of IMPDH. The invention also provides pharmaceutical compositions comprising the compounds of the invention which selectively inhibit parasitic IMPDH. In certain embodiments, the present invention relates to selective inhibition of | 10-18-2012 |
20120208191 | STAPHYLOCOCCUS DETECTION ASSAYS - Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various | 08-16-2012 |
20120202203 | SINGLE PROBE, MULTIPLE TEMPERATURE, NUCLEIC ACID DETECTION METHODS, KITS, AND COMPOSITIONS - Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification). | 08-09-2012 |
20120198576 | METHODS FOR MAKING EMBRYONIC CELLS, EMBRYOS, AND ANIMALS SENSITIZED TO STRESS - Embodiments of the invention are based upon the discovery that exposure of cleavage-stage embryos to a stress inducer, e.g. heat shock or chemical, renders the exposed embryos more sensitive to a secondary treatment with a stress inducer, e.g. heat shock or chemical inducer. Accordingly, the present invention is directed to methods for making embryos, embryonic cells arising from them, and animals and plants that are sensitized to stress, e.g. physiologic or chemical stressors. Methods of screening for inducers and inhibitors of stress using, as test model systems, embryonic cells, embryos, animals, and plants that are sensitized to stress are also disclosed. | 08-02-2012 |
20120190127 | SYSTEMS AND METHODS FOR DETERMINING PROCESS CONDITIONS IN CONFINED VOLUMES - Systems and methods for the determination of properties in confined (volumes are generally described. Generally, a gradient in at least one parameter (e.g., temperature) may be established across a plurality of confined volumes. In addition, at least one property (e.g., whether a crystal has been formed in the confined volume) of an interaction in at least one confined volume may be determined. In some embodiments, based upon the property determining step, a relationship between at least one parameter and at least one property may be determined. The confined volumes in which crystals may be contained may include, but are not limited to, droplets, microwells, and the like. | 07-26-2012 |
20120134978 | Cross-Linking of Superoxide Dismutase Monomers - A stabilized superoxide dismutase (SOD1) analogue, wherein the side chains of two amino acids on two different SOD1 monomers are connected is provided. A method of producing a stabilized superoxide disumutase (SOD1) analogue comprises reacting a first SOD1 monomer, a second SOD1 monomer, and a cross-linker. | 05-31-2012 |
20120114775 | Methods for the treatment or prevention of diabetes mellitus and other metabolic imbalances - The invention relates to methods for treatment and prevention of a metabolic imbalance, including diabetes mellitus and other related diseases or disorders, using an extract from a fruit of genus | 05-10-2012 |
20120101096 | Compounds and Methods for Treating Mammalian Gastrointestinal Microbial Infections - Described herein are compounds, and pharmaceutically acceptable salts and prodrugs thereof, which are useful as inhibitors of IMPDH. In certain embodiments, a compound of the invention selectively inhibits a parasitic IMPDH versus a host IMPDH. Further, the invention provides pharmaceutical compositions comprising one or more compounds of the invention. The invention also relates to methods of treating various parasitic and bacterial infections in mammals. Moreover, the compounds may be used alone or in combination with other therapeutic or prophylactic agents, such as anti-virals, anti-inflammatory agents, antimicrobials and immunosuppressants. | 04-26-2012 |
20120088275 | REAGENTS AND METHODS FOR PCR - Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32° C., and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified olgonucleotide being capable of binding to the 5′ ex-nuclease domains of DNA polymerases and, when included in a PCR or other primer-dependent DNA amplification reaction at a concentration, generally not more than 2000 nM, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3′ terminal mismatches, increasing polymerase selectivity against AT-rich 3′ ends, reducing scatter among replicates, suppressing polymerase 5′ exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides. | 04-12-2012 |
20110311971 | RT-LATE-PCR - An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described. | 12-22-2011 |
20110295011 | Conjugate Addition Reactions Using Bifunctional Cinchona-Alkaloid-Based Catalysts - One aspect of the present invention relates to quinine-based and quinidine-based catalysts. Another aspect of the present invention relates to a method of preparing a chiral, non-racemic compound from a prochiral electron-deficient alkene, comprising the step of: reacting a prochiral electron-deficient alkene with a nucleophile in the presence of a catalyst; thereby producing a chiral, non-racemic compound; wherein said catalyst is a derivatized quinine or quinidine. | 12-01-2011 |
20110229600 | SN-2 MYRISTATE-CONTAINING EDIBLE OIL - A nutritional fat or oil-based composition for increasing HDL cholesterol, decreasing LDL cholesterol and decreasing the LDL/HDL cholesterol ratio in human plasma is described. The composition can advantageously include at least 1% by weight myristic acid esterified at the sn-2 position in triglyceride molecules, includes between 10% and 40% by weight linoleic acid, and further includes between 30% and 65% by weight oleic acid and between 15% and 40% by weight total saturated fatty acids. The ratio of sn-2 myristic acid to sn-2 palmitic acid is typically greater than 1:1 and the sum of weight percentages for saturated, monounsaturated and polyunsaturated fatty acids equals 100%. In desirable cases, the composition is substantially cholesterol-free. | 09-22-2011 |
20110166227 | BALANCED MYRISTATE- AND LAURATE-CONTAINING EDIBLE OIL - Nutritional fat or oil-based compositions for increasing HDL cholesterol, decreasing total cholesterol (TC), LDL cholesterol and decreasing the LDL/HDL cholesterol ratio in human plasma are described. The compositions can advantageously include at least 10% of myristic acid and/or lauric acid in which at least 3% of said fatty acid composition is myristic acid and at least 3% is lauric acid; in some cases at least 1% by weight myristic acid and/or lauric acid is esterified at the sn-2 position in triglyceride molecules. The compositions also include between 5% and 40% by weight linoleic acid, and typically further include at least 10% by weight monounsaturated fatty acids and between 15% and 55% by weight total saturated fatty acids. The sum of weight percentages for saturated, monounsaturated and polyunsaturated fatty acids equals 100%. In desirable cases, the composition is substantially cholesterol-free. Also described are fat compositions and diets which are adapted to limit increases in blood triglycerides, total cholesterol, LDL, and VLDL, even in the presence of dietary cholesterol. | 07-07-2011 |
20110151018 | METHODS OF INSECT CONTROL - The present invention is directed towards methods of inhibiting thermo- and chemo-sensing in insects and pests by inhibiting TRPA1 ion gated channel or family members. The present invention is also directed towards methods of insect control by modulating the TRPA1 ion gated channel or family members. The methods are applicable to a wide variety of insects and pests including agricultural and horticultural pests. | 06-23-2011 |
20100163109 | MANIPULATION OF FLUIDS AND REACTIONS IN MICROFLUIDIC SYSTEMS - Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions. | 07-01-2010 |
20100152125 | Compositions And Methods For The Diagnosis, Treatment, And Prevention Of Amyotrophic Lateral Sclerosis And Related Neurological Diseases - One aspect of the invention relates to a method of treating or preventing a neurodegenerative disease, comprising the step of administering to a patient in need thereof a therapeutically effective amount of an inhibitor of the formation of advanced glycation end products. Another aspect of the invention relates to a proteasome activity-based screening assay to select compounds which may be useful for treating or preventing a neurodegenerative disease, and the materials used therein. Yet another aspect of the invention relates to molecules, and methods of use thereof, which bind at or adjacent to SOD-I Trp32, including molecules that bind in a site adjacent to SOD-I Trp32 whether or not it is oxidized, for treating or preventing neurodegenerative disease. | 06-17-2010 |
20100105866 | MICROFLUIDIC MANIPULATION OF FLUIDS AND REACTIONS - The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including microreactors for manipulating fluids and reactions. In some embodiments, structures and methods for manipulating many (e.g., 1000) fluid samples, i.e., in the form of droplets, are described. Processes such as diffusion, evaporation, dilution, and precipitation can be controlled in each fluid sample. These methods also enable conditions within the fluid samples (e.g., concentration) to be controlled. Manipulation of fluid samples can be useful for a variety of applications, including testing for reaction conditions, e.g., in crystallization, chemical, and biological assays. | 04-29-2010 |
20100022547 | Compounds and Methods for Treating Mammalian Gastrointestinal Parasitic Infections - One aspect of the present invention relates to compounds, and pharmaceutically acceptable salts and prodrugs thereof, that are useful as inhibitors of IMPDH. The invention also provides pharmaceutical compositions comprising the compounds of the invention which selectively inhibit parasitic IMPDH. In certain embodiments, the present invention relates to selective inhibition of | 01-28-2010 |
20090325222 | Tissue Sample Preparation and MALDI MS Imaging Thereof - Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis. | 12-31-2009 |
20090252817 | Methods for the treatment or prevention of Diabetes Mellitus and other metabolic imbalances - The invention relates to methods for treatment and prevention of a metabolic imbalance, including diabetes mellitus and other related diseases or disorders, using an extract from a fruit of genus | 10-08-2009 |
20090239215 | Clonable Tag for Purification and Electron Microscopy Labeling - The disclosure provides compositions and methods for heavy atom labeling of a target protein using a clonable tag. The clonable tag comprises a metal binding protein, or fragment thereof, such as metallothionein, which may be fused to a target protein of interest. The tag permits the target protein to be labeled with a heavy atom, such as gold, silver, or mercury, and thus permits visualization of the target protein by electron microscopy. Also provided are methods for purification of a target protein using metallothionein, or a fragment thereof, as an affinity tag. The metallothionein fusion may be purified on immobilized metal affinity chromatography (IMAC) column charged with a metal such as cadmium. | 09-24-2009 |
20080232545 | TOMOSYNTHESIS IMAGING SYSTEM AND METHOD - A system for three-dimensional tomosynthesis imaging of a target element is provided having an image acquisition element and a processor. The image acquisition element obtains a plurality of images of the target element from a plurality of angles and includes a radiation source that is positionable at a plurality of angles with respect to the target element and a radiation detector. The radiation detector is positioned so as to detect radiation emitted by the radiation source passing through the target element and determine a plurality of attenuation values for radiation passing through the target element to establish a radiation absorbance projection image of the target element for a particular radiation source angle. The processor is configured to apply an iterative reconstruction algorithm to the radiation absorbance projection images of the target element obtained from a plurality of radiation source angles to generate a three-dimensional reconstruction of the target element. The system can gain further accuracy where the iterative reconstruction algorithm is applied using cone-beam forward projection and back projection. | 09-25-2008 |
20080228000 | Cinchona Alkaloid-Catalyzed Asymmetric Mannich Reactions - The instability of carbamate-protected alkyl imines has greatly hampered the development of catalytic asymmetric Mannich reactions suitable for the synthesis of optically active carbamate-protected chiral alkyl amines. A highly enantioselective Mannich reaction with in situ generation of carbamate-protected imines from stable α-amido sulfones catalyzed by an organic catalyst has been developed. This reaction provides a concise and highly enantioselective route converting aromatic and aliphatic aldehydes into optically active aryl and alkyl β-amino acids. | 09-18-2008 |