Bioneer Corporation Patent applications |
Patent application number | Title | Published |
20150259690 | AMPHIREGULIN-SPECIFIC DOUBLE-HELICAL OLIGO-RNA, DOUBLE-HELICAL OLIGO-RNA STRUCTURE COMPRISING DOUBLE-HELICAL OLIGO-RNA, AND COMPOSITION FOR PREVENTING OR TREATING RESPIRATORY DISEASES CONTAINING SAME - An amphiregulin-specific double-stranded oligo siRNA structure comprises hydrophilic and hydrophobic compounds bound to the amphiregulin-specific double-stranded oligo siRNA by a simple covalent bond or a linker-mediated covalent bond so as to increase the in vivo stability and intracellular delivery efficiency of the double-stranded oligo siRNA, and to nanoparticles composed of the oligo siRNA structures. The amphiregulin-specific double-stranded oligo siRNA structure can increase the stability of the amphiregulin-specific double-stranded oligo siRNA without impairing the function of the amphiregulin-specific double-stranded oligo siRNA, and at the same time, can efficiently increase the membrane permeability of the double-stranded oligo siRNA. Thus, when the amphiregulin-specific double-stranded oligo siRNA structure is used, the amphiregulin-specific double-stranded oligo siRNA can be efficiently delivered into cells even when it is administered at a relatively low concentration, so that it can be effectively used for the prevention or treatment of respiratory diseases. | 09-17-2015 |
20150044683 | COMPOSITION FOR HOT-START REVERSE TRANSCRIPTION REACTION OR HOT-START REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION - A composition for hot-start reverse transcription reaction and a composition for reverse transcription PCR are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, MgCl | 02-12-2015 |
20140350233 | SIRNA CONJUGATE AND PREPARATION METHOD THEREOF - Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system. | 11-27-2014 |
20140350232 | SIRNA CONJUGATE AND PREPARATION METHOD THEREOF - Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system. | 11-27-2014 |
20140291583 | NANO COMPOSITE CONSISTING OF CARBON NANOTUBES AND METAL OXIDE AND METHOD FOR MANUFACTURING THE SAME - Provided are a method for manufacturing a carbon nanotube-metal oxide composite, and a carbon nanotube-metal oxide composite manufactured thereby, the method comprising: dispersing carbon nanotubes in a reductive solvent to prepare a dispersion liquid; adding a co-reducing agent and a metal precursor to the dispersion liquid to prepare a mixture liquid; and performing heat treatment on the mixture liquid to coat the metal precursor on the carbon nanotubes in a metal oxide form, so that there can be provided a carbon nanotube-metal oxide composite where metal oxide particles of several nm to several tens of nm are uniformly dispersed in carbon nanotubes or combined with surfaces of the carbon nanotubes in a coating type. | 10-02-2014 |
20140212919 | PROTEIN SYNTHESIS KIT, AND METHOD FOR EXPRESSING AND EXTRACTING PROTEINS USING AUTOMATIC EXTRACTION EQUIPMENT - Provided is a method of protein synthesis. The method of protein synthesis according to the present invention uses an automatic biological material purification apparatus including: a well plate kit; a heating part; and a magnetic field applying part, such that a plurality of target proteins may be more quickly and simply obtained as compared to target proteins obtained by using the existing method for expressing/purifying proteins through conventional cell culture, and a reproducible synthesis efficiency on the same proteins may be obtained due to no deviation between reaction wells. | 07-31-2014 |
20140087377 | METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT - The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide. | 03-27-2014 |
20140060400 | DEVICE FOR ELIMINATING HARMFUL SUBSTANCE - This invention relates to a device for effectively removing organic compounds from air by a carbon nanotube-catalyst composite having the two functions of an adsorption/catalytic incineration agent. The carbon nanotube-catalyst composite simultaneously adsorbs the organic compounds and completely decomposes them by a catalytic reaction, and the optimal reaction active temperature by catalytic incineration is low. The carbon nanotube-catalyst composite has a large surface area and has high adsorption performance and catalytic decomposition activity, and is thus applicable to filters that use the methods of adsorption and/or catalytic incineration. The device for removing organic compounds from air includes an adsorption/catalytic incineration reactor including the carbon nanotube-catalyst composite to remove organic compounds from air. | 03-06-2014 |
20140045244 | REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY - The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63 | 02-13-2014 |
20130236903 | AUTOMATIC NECLEIC ACID PURIFICATION APPARATUS AND METHOD FOR AEROSOL-PROTECTING - Disclosed is an automatic nucleic acid purification apparatus which can prevent pollution due to aerosol generated from a biological sample containing high concentration target nucleic acid when the biological sample containing the high concentration target nucleic acid is mixed with other biological sample containing low concentration target nucleic acid or not containing the target nucleic acid. Further, disclosed is an automatic nucleic acid purification apparatus which can be applied to all kinds of nucleic acid purification equipments for purifying a plurality of biological samples using a magnet rode or a multi-pipette block moving in two or three axial directions, and which can minimize pollution due to the aerosol generated from the biological sample containing high concentration target nucleic acid and also can obtain accurate results. | 09-12-2013 |
20130230860 | AUTOMATIC REAL-TIME PCR SYSTEM FOR THE VARIOUS ANALYSIS OF BIOLOGICAL SAMPLE - The present invention relates to an automatic real-time quantitative amplification system which can perform analysis of various biological samples, and more particularly to an automatic real-time quantitative amplification system in which a plurality of decks for respectively accommodating biological samples are put in a deck storing/transferring device, whereby it is possible to automatically analyze an amount or existence of a target substance containing a target nucleic acid in the biologic sample, such as a particular gene, a particular, a particular pathogenic bacterium and a particular protein, by amplifying the target nucleic acid purified by some processes of purification, purification after culture, or purification after reaction of the target substance contained in the bio-logical sample and then checking an amount of the amplified target nucleic acid. | 09-05-2013 |
20130210133 | LOW HEAT CAPACITY COMPOSITE FOR THERMAL CYCLER - Provided is a low heat capacity composite for a thermal cycler . The low heat capacity composite of the present invention is a low heat capacity composite for a thermal cycler capable of overcoming difficulty in manufacture and reproducibility due to uniqueness of the existing PCR thermal cycler only. The low heat capacity composite of the present invention can reduce the cost of raw material and retain excellent heat property due to the improvement in low heat capacity and physical and mechanical properties, thereby remarkably shortening PCR reaction time and saving energy when used as a thermal block for a thermal cycler. | 08-15-2013 |
20130157275 | OLIGONUCLEOTIDE MARKER AND METHOD FOR IDENTIFYING THE SAME - Provided are an oligonucleotide marker and a method of identifying a material using the same. The oligonucleotide marker makes it possible to analyze a trace amount of the material with high precision within a short time, has improved solubility in an oily solvent, and can improve a detection method such that the oligonucleotide marker can be detected within 2 hours. The oligonucleotide marker can label various products, including oil products and petroleum products, works of art and collections, and can also be used to conduct criminal investigations. | 06-20-2013 |
20130115686 | METHOD OF MANUFACTURING MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE AND ANALYTIC MICRO CHAMBER PLATE, ANALYTIC MICRO CHAMBER PLATE AND APPARATUS SET FOR MANUFACTURING ANALYTIC MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE - The present invention relates to a micro chamber plate, and more particularly, to an analytic micro chamber plate in which a plurality of reaction solutions including a primer or probe selectively reacting with a nucleic acid react with each other without cross-contamination to measure and analyze a fluorescence level in real-time so as to analyze biological sample solution including a large amount of nucleic acids. Also, the present invention relates to a method of manufacturing the analytic chamber plate. Also, the present invention relates to a method of manufacturing a micro chamber plate with a built-in sample used for manufacturing the analytic chamber plate. Also, the present invention relates to an apparatus set for manufacturing the micro chamber plate with a built-in sample. | 05-09-2013 |
20130096288 | siRNA conjugate and preparation method thereof - Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system. | 04-18-2013 |
20130043191 | AUTOMATIC BIOLOGICAL SAMPLE PURIFICATION APPARATUS EQUIPPED WITH MAGNETIC FILED APPLYING PART, METHOD OF EXTRACTING TARGET SUBSTANCE FROM BIOLOGICAL SAMPLE, AND PROTEIN EXPRESSION AND PURIFICATION METHOD - Disclosed is an automatic purification apparatus for isolating target substances from multiple biological samples, and more particularly to an automatic biological sample purification apparatus equipped with a magnetic field applying part, in which the magnetic field applying part for purifying biological samples and a heating part are integrally formed with each other so as to be movable up and down. | 02-21-2013 |
20120223017 | OIL PURIFICATION METHOD AND APPARATUS WITH POROUS MEMBRANE - Provided is a method of purifying oil by which nano particles are effectively removed from the oil. According to the oil purifying method, oil is effectively purified at a high temperature using a carbon nanostructure-metal or -metal oxide composite nano porous membrane composed of a carbon nanostructure-metal composite | 09-06-2012 |
20120216597 | SAMPLE PRECONCENTRATOR - There is provided a sample preconcentrator. The sample preconcentrator in which a sample gas injection port is coupled to a dried gas supply source and a gas analysis system to concentrate a sample gas comprises a sample concentrating unit containing an absorbent that is composed of carbon nanotube-metal nanocomplexes; a conduit switching valve for selectively coupling the sample gas injection port to the dried gas supply source and the gas analysis system and controlling the absorption and desorption of the sample gas from the sample concentrating unit; and a plurality of conduits for connecting the sample gas injection port, the dried gas supply source, the gas analysis system, the sample concentrating unit and the conduit switching valve. | 08-30-2012 |
20120135394 | APPARATUS FOR INTEGRATED REAL-TIME NUCLEIC ACID ANALYSIS, AND METHOD FOR DETECTING A TARGET NUCLEIC ACID USING SAME - Provided are an apparatus for integrated real-time nucleic acid analysis and a method for detecting target a nucleic acid using the same, and more particularly an integrated real-time nucleic acid analysis for simultaneously performing qualitative analysis or quantitative analysis on genes from various kinds of plural biological samples and a method for detecting target a nucleic acid using the same. The apparatus for integrated real-time nucleic acid analysis and the method for detecting target a nucleic acid using the same according to the present invention, perform tests of various targets required from various samples through a single step promptly and accurately, and thus, can be efficiently used by hospitals or the like needing to rapidly diagnose diseases. | 05-31-2012 |
20120114535 | COMPOUND FOR INHIBITING ACTIVITY OF RIBONUCLEASE, AND CONTAINER FOR STORING NUCLEIC ACID CONTAINING THE SAME - Provided are an RNase activity inhibitory compound to effectively control the activity of the RNase promoting degradation of extracted RNAs and, in addition, a sample storage container including the same. The RNase activity inhibitory compound and the sample storage container according to the present invention may be effectively used to store RNAs during RNA extraction or the extracted RNAs. | 05-10-2012 |
20120108803 | SIRNA CONJUGATE AND PREPARATION METHOD THEREOF - Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system. | 05-03-2012 |
20120000845 | NANOPOROUS FILMS AND METHOD FOR MANUFACTURING THE SAME - Provided is a carbon nanostructure-metal composite nanoporous film in which a carbon nanostructure-metal composite is coated on one surface or both surfaces of a membrane support having micro- or nano-sized pores. A method for manufacturing a carbon nanostructure-metal composite nanoporous film, includes: dispersing a carbon nanostructure-metal composite in a solvent at the presence of a surfactant and coating the carbon nanostructure-metal composite on one surface or both surfaces of a membrane support; and fusing the metal on the membrane support by heating the coated membrane support. The metal in carbon nanostructure-metal composite nanoporous film melts at a low temperature since a size of a metal of the carbon nanostructure-metal composite is several nm to several-hundred nm. | 01-05-2012 |
20110177563 | HIGH THROUGHPUT DEVICE FOR PERFORMING CONTINUOUS-FLOW REACTIONS - A high-throughput device is structured to perform a continuous-flow reaction, e.g., a polymerase chain reaction (PCR) requiring repetitive temperature control in a timely fashion. | 07-21-2011 |
20110159579 | THERMAL CYCLING REACTION BLOCK AND CONTINUOUS REAL-TIME MONITORING APPARATUS USING THE SAME - Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part. | 06-30-2011 |
20110081546 | NANOCOMPOSITES CONSISTING OF CARBON NANOTUBE AND METAL AND A PROCESS FOR PREPARING THE SAME - The present invention relates to a method for preparing a nano-composite comprising carbon nanotube and metal, more precisely a method for preparing a carbon nanotube-metal composite comprising the steps of preparing a dispersion solution by dispersing carbon nanotube in a reductive solvent; preparing a mixed solution by adding a stabilizer and a metal precursor; and reducing the metal precursor by heating the mixed solution, and a carbon nanotube-metal composite prepared by the same. | 04-07-2011 |
20110054162 | Silica Magnetic Particles Having a Spherical Form and a Process for Preparing the Same - The present invention relates to silica magnetic particles having a spherical form and a process for preparing the same. The silica magnetic particles prepared according to the present invention, which are silica particles that includes the magnetic particles and additionally have the functional group on the surfaces, has an advantage that the particle size distribution is uniform. Further, the silica magnetic particles prepared according to the present invention can be used as a reagent for separating biomaterials and a reagent for detecting biomaterials. | 03-03-2011 |
20110009608 | AUTOMATIC REFINING APPARATUS, MULTI-WELL PLATE KIT AND METHOD FOR EXTRACTING HEXANE FROM BIOLOGICAL SAMPLES - The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which the magnetic particles are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting nucleic acids from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of nucleic acid, protein, and the like from biological samples. | 01-13-2011 |
20110008845 | PRIMERS FOR PCR AMPLIFICATION COMPRISING A BASIC PARTS WITHIN THE PRIMER SEQUENCES - The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time. | 01-13-2011 |
20100261184 | Micro-Chamber Plate, Manufacturing Method Thereof - The present invention relates to a micro-chamber plate and a manufacturing method of the same, more precisely a micro-chamber plate facilitating real-time measurement and analysis of fluorescence obtained from the reaction of multiple reaction solutions containing primers or probes selectively binding to each corresponding gene without cross-contamination in order to analyze biological samples containing numbers of genes and a manufacturing method of the same. | 10-14-2010 |
20100203025 | LACTIC ACID BACTERIA ISOLATED FROM MOTHER'S MILK WITH PROBIOTIC ACTIVITY AND INHIBITORY ACTIVITY AGAINST BODY WEIGHT AUGMENTATION - The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a | 08-12-2010 |
20090325291 | METHOD OF PREPARING siRNAs FOR SELECTIVE INHIBITION OF TARGET mRNA ISOTYPES - A method of preparing siRNAs for selective inhibition of target mRNA isotypes comprises: dividing target mRNA isotypes intended to inhibit the expression thereof and non-target mRNA isotypes from the mRNA isotypes of a gene; allotting a common location information region (A) of exons on genome DNA corresponding to the target mRNA isotypes; allotting a location information region (B) present specifically in exons of genome DNA corresponding to target mRNAs by excluding the location information region of exons on genome DNA corresponding to non-target mRNA from the location information region (A); determining base sequences in the target mRNAs corresponding to the location information region (B); and obtaining siRNA sequences for inhibiting the determined base sequences specifically. The method of the present invention can be used to prepare siRNAs for selective inhibition of specific target mRNA isotypes in a gene having several isotypes by alternative splicing, and enables siRNA design for all the genes in genome, making good tool for functional genomics study. | 12-31-2009 |
20090155904 | METHOD OF INHIBITING EXPRESSION OF TARGET MRNA USING SIRNA CONSISTING OF NUCLEOTIDE SEQUENCE COMPLEMENTARY TO SAID TARGET MRNA - A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA. As a result, a researcher or an experimenter can analyze patterns of a relative binding energy on base sequences of unknown siRNA without actual experiments to determine whether the siRNA is effective or ineffective rapidly, thereby design and production efficiency of siRNA can be maximized and target mRNA can be effectively inhibited with efficient siRNA to the target mRNA. | 06-18-2009 |
20090023906 | DRIED OLIGONUCLEOTIDE COMPOSITION AND METHOD OF PRODUCING THE SAME - The present invention relates to a dried oligonucleotide composition and a method for producing the same. More specifically, it relates to a dried oligonucleotide composition produced by the steps comprising adding a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition, in order to prevent the oligonucleotide from being separated and lost during manufacturing and distributing the dried oligonucleotide composition, optionally adding a non-reactive dye substance, and drying the resulting solution. The dried oligonucleotide composition of the present invention can be prevented from being separated and lost during manufacturing step, or transporting step after packaging, and the presence or absence of the oligonucleotide in the storage container can be easily confirmed with naked eyes. Accordingly, unnecessary labor waste and time waste caused by the separation of the oligonucleotide upon experiment can be overcome. | 01-22-2009 |