BIOGENOMICS LIMITED Patent applications |
Patent application number | Title | Published |
20160060291 | PROCESS FOR RENATURATION OF POLYPEPTIDES - The invention relates to method of refolding of proteins from a solution containing the protein in predominantly misfolded, aggregated form. The method involves denaturation and reduction of the protein of interest. The denatured and reduced preparation is subjected to removal of reducing agent in denaturing condition and at low pH to prevent the misfolding of the protein. The protein preparation is subjected to refolding followed by removal of the refolding buffer components. | 03-03-2016 |
20160039868 | PROCESS FOR ISOLATION AND STABILISATION OF KEY INTERMEDIATES FOR HIGH EFFICIENCY REFOLDING OF RECOMBINANT PROTEINS - A process for obtaining a refolded recombinant protein from an unfolded recombinant protein present in inclusion bodies isolated from wet cells harvested from a cell culture is provided. The process includes a) reducing the inclusion bodies by treating the inclusion bodies with a reducing buffer, to obtain reduced inclusion bodies; b) obtaining stable first intermediate state I | 02-11-2016 |
20150376228 | PROCESS FOR HIGH EFFICIENCY REFOLDING OF RECOMBINANT PROTEINS - A process of refolding a recombinant protein from inclusion bodies (IBs) formed inside a host cell, is provided. The process includes homogenising a wet cells slurry to obtain a cell lysate; incubating the cell lysate with a reducing agent to obtain a reduced cell lysate; isolating reduced IBs from the reduced cell lysate to obtain isolated IBs; solubilising the isolated IBs with a denaturing agent to obtain solubilised IBs; and subjecting the solubilised IBs to a unit process of refolding to obtain a refolded recombinant protein. | 12-31-2015 |
20150118754 | STABLE EXPRESSION SYSTEM FOR EUKARYOTIC CELLS - The present invention related to a polynucleotide sequence and an expression vector comprising at least one gene encoding a stress resistance protein, at least one gene encoding a selection marker, at least one gene encoding an expression protein, at least one matrix attachment region and a transcription terminator, all of which are operably connected to each other. The present invention further relates to a host cell comprising the expression vector. The present invention also relates a method of producing a cell line. | 04-30-2015 |
20150105537 | CODON OPTIMIZED SEQUENCE FOR AN ANTIVIRAL PROTEIN - A codon optimized nucleic acid sequence for Interferon Alpha-2a is provided which can be used for expression of Interferon Alpha-2a in | 04-16-2015 |