| 20100240098 | PROCESS FOR PRODUCING MATURE RECOMBINANT MITE GROUP I ALLERGEN - The present invention relates to a process for producing mature recombinant Mite Group I allergen comprising the steps of a) providing an allergen expression system in the form of a host cell selected from the group of yeasts containing a vector comprising cDNA encoding pro-allergen, b) cultivating the allergen expression system at a cultivation pH of between 5.5 and 7.5 for a cultivation period to express pro-allergen to obtain a cultivation mixture containing pro-allergen, c) adjusting the pH of the cultivation mixture to a maturation pH of between 3.5 and 5.0, d) maintaining the cultivation mixture at the maturation pH for a maturation period to convert the pro-allergen into mature allergen to obtain a product mixture, and e) subjecting the product mixture to a purification method to isolate mature allergen from the product mixture. | 09-23-2010 |
