University of Oulu Patent applications |
Patent application number | Title | Published |
20140342398 | Method for producing disulfide bond containing proteins in a prokaryotic cytoplasm - The present invention relates to a method for producing natively folded disulfide bond containing proteins in a prokaryotic host. The method comprises that in the cytoplasm of a prokaryotic cell is expressed protein(s) of interest that naturally contain disulfide bonds and naturally occurring or inverted transmembrane enzyme, wherein the cysteines of the active site(s) are naturally or after genetic engineering located towards the prokaryotic cytoplasm. The enzyme is selected from the group of VKOR, inverted VKOR (iVKOR) and inverted Dsb B (iDsb B). In the prokaryotic cell is also expressed cytoplasmic DsbA or a corresponding protein being capable of providing electrons to the active site(s) of VKOR, iVKOR or iDsbB. The invention relates also to a prokaryotic host cell and a vector system for producing natively folded disulfide bond containing proteins. | 11-20-2014 |
20140050674 | Method for Improving Penetration or Long Term Adhesion of Compositions to Dental Tissues and Compositions Usable in Said Method - This invention concerns a method of improving penetration of compositions to dentin, enamel, dental pulp or cement and dental compositions for restoration or decoration of teeth, for use in pulp medication comprising, for root canal disinfection and/or obturation and for desensitizing. This invention also concerns uses of DMSO for improving the bond strength of dental composition and in preparing dental compositions. Further the invention concerns a new method of treating teeth so that penetration of components is increased. | 02-20-2014 |
20120202245 | Method for Producing Natively Folded Proteins in a Prokaryotic Host - The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds. | 08-09-2012 |
20100124548 | Pharmaceutical product - The present invention relates to methods and products for the treatment of any disorder or condition, which is associated with abnormal amount of non-collagenous protein, or abnormal oligomerization or dysfunction of non-collagenous protein, such as adiponectin in the blood circulation and/or tissue of a patient. The treatment comprises that functional form of the non-collagenous protein is adjusted in the blood circulation and/or tissue of the patient substantially to the level it is in the blood circulation and/or tissue of a healthy person, by using lysyl hydroxylase and/or glycosyl-transferase activities of LH3 or other lysyl hydroxylase to modify the non-collagenous protein to HMW or other functional form. | 05-20-2010 |
20080227105 | Method for diagnosing diseases - An improved method for diagnosing autoimmune or genetic diseases is provided in this disclosure. In particular, a method to diagnose diseases affecting the tissues of organs selected from the group of spleen, brain, heart, kidney, thyroid, eye, skin, intestine, liver, pancreas, adrenal gland, prostate and lungs or from the tissues selected from the group of muscles and bones or other tissues. More specifically, the present invention provides an improved method for diagnosing diseases affecting neuromuscular junctions. | 09-18-2008 |