Bio-Rad Laboratories, Inc. Patent applications |
Patent application number | Title | Published |
20160104806 | HEATED IMAGE SENSOR WINDOW - An image sensor assembly having a sensor window positioned in front of an image sensor, having structure and/or characteristics to prevent the formation of condensation on the sensor window. Structure to prevent the formation of condensation includes thin films which can have anti-condensation, anti-reflective, electrically conductive, and/or thermally conductive properties. The sensor window can further have a textured surface to displace water so as to avoid condensation formation on the window surface. The sensor window, and in some embodiments a frame, can be maintained at an elevated temperature proximate to the image sensor during operation to prevent the formation of condensation. | 04-14-2016 |
20160103596 | CHROMATOGRAPHY CONFIGURATION INTERFACE - Systems and methods that facilitate the setup and visualization of a configurable process, for example a chromatography experiment to be conducted using a configurable chromatography system. A user interface displays category regions representing sections of a process, and displays a plurality of selectable items representing components appropriate for placement in the category regions. A user interface control causes selection of one of the selectable items and placement of the selected item into one of the category regions, to define a selected process configuration. The user interface control may be a scrolling control that causes a set of selectable items appropriate for placement in the respective category region to scroll through the category region. When the computer displaying the user interface is also connected to the system being configured, the user interface may also facilitate setup, monitoring, and control of the resulting process. | 04-14-2016 |
20160034653 | SYSTEM AND METHOD FOR RECOVERING FROM A LARGE OUT-OF-CONTROL CONDITION IN A CLINICAL DIAGNOSTIC PROCESS - Disclosed are a system and method to recover from a large out-of-control condition in a clinical diagnostic process. Upon detection of a large out-of-control condition, a CUSUM rule is designed to predict if specimen test data was produced during the occurrence of that condition. Specimen test data is re-evaluated and the CUSUM rule is applied to determine whether to accept or reject the test results. Upon completion of the re-evaluation, old test results identified as being invalid are replaced with the new, re-evaluated results and the new results are published if publication did not occur previously. | 02-04-2016 |
20160033541 | CALIBRATION PROCESS AND SYSTEM - An improved calibration process for a medical testing machine. The machine automatically recognizes that a package of calibration material has been inserted into it, and performs a calibration sequence to ascertain a calibration parameter to be used in performing future tests with the medical testing machine. The calibration package may include machine-readable indicators that the package is to be used for calibration, and of a calibration setpoint of a calibration material in the package. A calibration material may be stored in a lyophilized state in the package, and the medical testing machine may automatically reconstitute the lyophilized material. | 02-04-2016 |
20150316479 | SERIES ABSORBANCE GLASS AND INTERFERENCE FILTERS - An imaging assembly and processing system that includes a sample platform having a target region which can hold a sample, where the sample can be marked with fluorescent or phosphorescent markers. The imaging assembly can have an excitation light module proximate to the sample platform that emits light to excite the markers, and a lens module positioned to receive emission light from excited markers in target region. At least one series filter assembly or interference filter can be arranged in front of, behind, or both in front of and behind the lens module. The assembly includes a light sensor and a processor and imaging module configured to process data captured by the light sensor. Images of the sample are generated based on the emission light from the sample that transmit through and are filtered by the lens assembly and series filter assembly or interference filter. | 11-05-2015 |
20150286043 | ANNULAR ILLUMINATION STRUCTURE - The present invention relates in general to microscopy systems and the illumination of target regions of the microscopy system with an annular illumination structure. The annular illumination structure can partially illuminate by quadrant, and can include a plurality of LEDs as light sources located in the annular structure that surround the lens or camera of an optical imaging and capture system. | 10-08-2015 |
20150031139 | METHOD AND DEVICE FOR STUDYING TRANSPORT OF AN AGENT ACROSS A BILAYER MEMBRANE IN BIOANALYTICAL SENSOR APPLICATIONS - The present invention provides a method for studying transport of an agent across a membrane comprising the steps a) providing at least one surface with a bilayer structure tethered to the surface, said bilayer structure comprising a detection volume, b) contacting the bilayer with at least one agent to be analyzed, and c) detecting a change in refractive index in the detection volume resulting from transportation of the agent across the membrane. Further there is provided a device comprising a) at least one surface, b) at least one bilayer structure tethered to the surface, and c) at least one sensor capable of detecting a change in refractive index in a detection volume, wherein the bilayer structure encloses a first volume of the detection volume and wherein the volume not enclosed by the bilayer structure but within the detection volume is a second volume and wherein the ratio between the first volume and second volume is above about 0.001. | 01-29-2015 |
20150017648 | DEVICES, SYSTEMS AND METHODS FOR THERMAL CONTROL OF DROPLET DETECTION - A droplet detection system comprises a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel can meet at an intersection. The sample reservoir can include a sample or partition thereof. During use, an emulsion comprising one or more droplets can be generated at the intersection. The emulsion flows from the intersection along a detection channel to a collection reservoir. A detection assembly that is coupled to at least a portion of the detection channel is configured to detect a signal from the one or more droplets. An energy providing member can be in thermal communication with at least one of the carrier fluid reservoir, the sample reservoir, the intersection, the detection channel and the detection assembly. The energy providing member is configured to transfer energy to the emulsion. | 01-15-2015 |
20140339084 | INSTRUMENT FOR INDEPENDENT ELECTROTRANSFER IN MULTIPLE CASSETTES - Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes. | 11-20-2014 |
20140336068 | MAGNETIC IMMUNO DIGITAL PCR ASSAY - The present invention provides methods of detecting an antigen in a sample comprising contacting the sample with affinity agents to form an antigen-affinity agent-label complex, separating the antigen-affinity agent-label complex from uncomplexed components, partitioning label from the separated sample into multiple partitions, and detecting the presence of the label in one or more partitions. Compositions and kits for detecting an antigen in a sample are also provided. | 11-13-2014 |
20140329263 | Stabilization of Labile Analytes in Reference Materials - Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same. | 11-06-2014 |
20140327758 | ADJUSTABLE DIGITAL MICROSCOPE DISPLAY - The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus. | 11-06-2014 |
20140327757 | ADJUSTABLE DIGITAL MICROSCOPE DISPLAY - The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. | 11-06-2014 |
20140327349 | Drawer Handle Mechanism - A drawer apparatus having a drawer chassis and a drawer adapted to move relative to the drawer chassis from a closed position to an open position. The drawer has first and second lateral sides. A lid assembly is moveable from an upward position to a downward position relative to the drawer chassis. The lid assembly has first and second roller members. A first lever arm is rotatable about a first pivot on the first lateral side. The first lever arm has a first surface for moveably engaging with the first roller member. A second lever arm is rotatable about a second pivot on the second lateral side. The second lever arm has a second surface for moveably engaging with the second roller. A handle connects the first and second lever arms. The handle is adapted to move the first and second lever arms respectively about the first and second pivots to move the lid assembly from the downward position to the upward position. | 11-06-2014 |
20140326080 | Ultrasonically Cleaned Low-Pressure Filter - A filter device having a sonotrode connected to a filtration assembly. The filtration assembly has a filter body having a fluid passage. The filter body has a filter across the fluid passage. The filter assembly has a resonant frequency and the sonotrode is adapted to vibrate the filter assembly at or near the resonant frequency. | 11-06-2014 |
20140323332 | MULTIPLEX HEPATITIS B ASSAY - Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection. | 10-30-2014 |
20140312247 | FLUORESCENCE IMAGER ON A MOBILE DEVICE - Systems and methods for substantially simultaneously exciting a fluorescently labeled specimen and capturing fluorescent light emitted therefrom using a smartphone, tablet, or similar mobile computing device, are disclosed herein. The system includes a light-emitting diode (“LED”) light source coupled with the smartphone to excite the specimen and an imaging device coupled with the smartphone to capture fluorescent light emitted from the specimen. The system further includes a hood adapted to be coupled with the smartphone that has an excitation filter configured to produce a first wavelength of electromagnetic radiation to strike the specimen when light from the LED light source passes through it and an emission filter configured to receive the light emitted from the specimen and to produce a second wavelength of electromagnetic radiation to be captured by the imaging device. | 10-23-2014 |
20140311909 | PROTEIN STANDARD - Disclosed herein is a protein standard for gel electrophoresis. The standard may be detected using multiple modalities. These modalities include, for example, observation of color or fluorescence arising from dyes, porphyrins, or haloalkylated tryptophan residues covalently linked to proteins of the standard; or detection of fluorescence or chemiluminescence arising from antibodies or other binding partners bound to proteins of the standard through polypeptide epitopes or affinity tags. The protein standard comprises multiple protein sets, each set corresponding to a different gel band, and proteins within a set may be detectable with one or more modalities. | 10-23-2014 |
20140309128 | DIGITAL ASSAYS FOR MUTATION DETECTION - Provided herein are methods, compositions, and kits for detecting alleles using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for detecting allelic variants using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for determining a polymerization error rate. | 10-16-2014 |
20140304964 | PROBE HEIGHT FIXTURE PRODUCT PROFILE - Systems, methods, and apparatuses are provided for using a calibration fixture adapted for use with a measurement instrument having a sample probe needle. The calibration fixture can be used for calibrating a maximum depth the sample probe needle will travel to for optimal aspiration of samples within the wells of one or more of multiple different microplates. The calibration fixture can include multiple cavities to calibrate the sample probe needle height for each of multiple different microplates. Each cavity has a cavity height that corresponds with a known depth of the wells disposed within each different microplate. | 10-16-2014 |
20140293412 | MODULAR MICROSCOPE CONSTRUCTION - A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination. | 10-02-2014 |
20140273251 | COLLOIDAL COOMASSIE STAIN - Colloidal formulation for staining proteins and methods of their use are provided. | 09-18-2014 |
20140262785 | POLYACRYLAMIDE GELS FOR RAPID CASTING, BLOTTING, AND IMAGING, WITH STORAGE STABILITY - Hand cast gels, and solutions for their preparation, are described. | 09-18-2014 |
20140261868 | BOTTLE PRESSURIZATION DELIVERY SYSTEM - A container assembly for use with a high-pressure liquid chromatography (HPLC) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the HPLC instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the HPLC instrument. As a pressurized gas in the interstitial volume increases, fluid medium flows out of the port connected to the internal fluid bag and container assembly at a positive pressure. A system incorporating the container assembly, and method of use of the same, are also disclosed. | 09-18-2014 |
20140260970 | DEGASSING OF A LIQUID TO CONTROLLED LEVEL IN COMPOSITE TUBE - Disclosed herein is an apparatus for debubbling or degassing a liquid. The apparatus includes a tube with a hollow lumen and a gas-permeable, liquid-impermeable wall surrounding the lumen. Also disclosed is a method for performing the debubbling or degassing, wherein the liquid is passed through the tube and gas is allowed to escape through the wall of the tube. The application additionally discloses a system for debubbling or degassing a liquid, wherein a pump is connected to the upstream end of the tube and a device to impede the flow of liquid out the tube is connected to the downstream end. | 09-18-2014 |
20140256585 | REPETITIVE REVERSE TRANSCRIPTION PARTITION ASSAY - Methods, compositions, and kits are provided for quantifying RNA molecules in a partitioned sample via multiple reverse transcription reactions. | 09-11-2014 |
20140223234 | SYSTEM AND METHOD FOR ANALYZING A QC STRATEGY FOR RELEASING RESULTS - A method analyzes a quality control strategy. A quality control rule can define quality control events and specifying a control limit for determining whether a quality control event passes or fails. The quality control rule and a number of patient samples tested between quality control events can be received. A first expected number of correctible errors when a quality control event fails can be computed based on the quality control rule and the number of patient samples tested between quality control events. A second expected number of final errors that are not correctible when a quality control event fails can be computed based on the quality control rule and the number of patient samples tested between quality control events. An assessment of the quality control rule can include the first expected number of correctible errors and the second expected number of final errors as separate values. | 08-07-2014 |
20140221239 | SYSTEM FOR DETECTION OF SPACED DROPLETS - System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets. | 08-07-2014 |
20140220607 | SIGNAL AMPLIFICATION FOR IMMUNOASSAYS BY USE OF AVIDIN-BIOTIN LINKAGES - In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites. | 08-07-2014 |
20140220586 | FILTERING SMALL NUCLEIC ACIDS USING PERMEABILIZED CELLS - Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described. | 08-07-2014 |
20140220577 | Methods of Using Improved Polymerases - This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase. | 08-07-2014 |
20140218719 | DETECTION SYSTEM WITH ONE-PIECE OPTICAL ELEMENT TO CONCENTRATE AND HOMOGENIZE LIGHT - Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion. | 08-07-2014 |
20140216579 | SYSTEM FOR EMULSION ASPIRATION - System, including apparatus and methods, for aspirating at least a portion of an emulsion from a well using a tip. In some embodiments, the tip may have a flat end and an inlet surrounded by the flat end. The well may have a floor with one or more surface features that prevent uninterrupted circumferential contact of the flat end of the tip with any region of the floor. In some embodiments, the tip may not have a flat end. In some embodiments, the well may have a port that guides the tip to the floor with the tip slanted with respect to the floor. Methods of making a device that includes the well are also disclosed. | 08-07-2014 |
20140191117 | Chromatography System with LED-Based Light Source - A detector system having a single emitter body. The emitter body has a plurality of light emitting diodes (LEDs) for emitting a plurality of wavelengths. Each LED adapted to emit a different wavelength of light. A broadband filter is adapted to receive the plurality of wavelengths. A detector arrangement adapted to receive the plurality of wavelengths filtered by the broadband filter. A controller adapted to control the plurality of LEDs and detector arrangement. | 07-10-2014 |
20140186938 | SERIAL-LINE-SCAN-ENCODED MULTI-COLOR FLUORESCENCE MICROSCOPY AND IMAGING FLOW CYTOMETRY - A system for performing high-speed, high-resolution imaging cytometry includes a scanning region that is illuminated by light including at least first and second wavelength bands. The system also includes a cell transport mechanism that transports a cell through the scanning region such that the cell is illuminated. The system further includes a set of at least one linear light sensor, and an optical system that selectively directs light emitted from the cell to two portions of the linear light sensor set such that emitted light in a third wavelength band is primarily directed to a first portion of the linear light sensor set, and emitted light in a fourth wavelength band is primarily directed to a second portion of the linear light sensor set. The system repeatedly takes readings of light falling on the linear light sensor set while the cell is transported through the scanning region. | 07-03-2014 |
20140179808 | Detection of Ovarian Carcinoma by Assay for Autoantibodies to Multiple Antigens - Assays and reagents for the detection of autoantibodies to detect ovarian carcinomas are provided. | 06-26-2014 |
20140179544 | COMPOSITIONS, SYSTEMS AND METHODS FOR DROPLET FORMATION, SPACING AND DETECTION - The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids. | 06-26-2014 |
20140171341 | MULTIPLEXED DIGITAL ASSAYS - System, including methods and apparatus, for performing a multiplexed digital assay. | 06-19-2014 |
20140170736 | METHODS AND COMPOSITIONS FOR USING OILS FOR ANALYSIS AND DETECTION OF MOLECULES - Oil compositions and methods for use in droplet formation or in spacing of droplets are disclosed. The oil compositions may include a combination, such as a silicone oil and a fluorine-containing compound. The droplets can contain analytes (e.g., nucleic acids) and may be used for molecular reactions (e.g., digital PCR) and detection. | 06-19-2014 |
20140170729 | SSO7-POLYMERASE CONJUGATES WITH DECREASED NON-SPECIFIC ACTIVITY - Improved Sso7-polymerase conjugate proteins are provided. | 06-19-2014 |
20140170706 | LOW-MASS SAMPLE BLOCK WITH RAPID RESPONSE TO TEMPERATURE CHANGE - A sample block for use in the polymerase chain reaction, DNA sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells. | 06-19-2014 |
20140170665 | SYSTEMS AND METHODS FOR STABILIZING DROPLETS - This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir. | 06-19-2014 |
20140162266 | METHODS FOR POLYMERASE CHAIN REACTION COPY NUMBER VARIATION ASSAYS - This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample. | 06-12-2014 |
20140157859 | CALIBRATION PROCESS AND SYSTEM - An improved calibration process for a medical testing machine. The machine automatically recognizes that a package of calibration material has been inserted into it, and performs a calibration sequence to ascertain a calibration parameter to be used in performing future tests with the medical testing machine. The calibration package may include machine-readable indicators that the package is to be used for calibration, and of a calibration setpoint of a calibration material in the package. A calibration material may be stored in a lyophilized state in the package, and the medical testing machine may automatically reconstitute the lyophilized material. | 06-12-2014 |
20140138248 | POLYACRYLAMIDE ELECTROPHORESIS GELS WITH PROTECTION AGAINST OXYGEN EXPOSURE - The detrimental effect of oxygen exposure on the formation of polyacrylamide electrophoresis gels in oxygen-permeable plastic cassettes is reduced or eliminated either by the use of an oxygen barrier material over the surfaces of the plastic walls of the cassette through which oxygen would otherwise pass into the cassette cavity, or by the incorporation of an oxygen scavenger in the plastic from which the cassette walls are made, or both. | 05-22-2014 |
20140134597 | CELLULAR HEMOGLOBIN A1C QUALITY CONTROLS - Intact erythrocytes with selected and often elevated levels of hemoglobin A1c (HbA1c) for use as quality controls for HbA1c assays and assay instruments are prepared by hypotonic dialysis of erythrocytes from a healthy mammal to permeabilize the erythrocyte membranes, infusion of the permeabilized erythrocytes with hemoglobin A1c, and de-permeabilization of the infused erythrocytes. Quality controls of essentially any level of HbA1c can be prepared in this manner and once prepared will be useful for monitoring the entire assay procedure, including the lysis of the erythrocytes in a typical sample. | 05-15-2014 |
20140106359 | DIGITAL TELOMERASE ASSAY - Digital assay system, including methods and apparatus, for determining a presence or activity of telomerase in a sample. | 04-17-2014 |
20140100791 | PATIENT-BASED RESULTS DISPLAY - A medical testing machine provides improved recall and display of the outcomes of tests performed by the machine. Test outcomes and other information are stored in mass storage directly accessible by the medical testing machine. In one aspect, outcomes of tests relating to a particular patient may be recalled. The system may be especially useful for tests that may be performed multiple times for a particular patient over a period of time, for example testing for HbAlc hemoglobin levels in diabetes patients. According to another aspect, the medical testing machine may store an accession number for each test outcome, and may enable a user to display as a group test outcomes having the same accession number. The system may store further information and associate it with particular test outcomes, for example calibration information. The system may also store a set of rules under which each test instance was run. | 04-10-2014 |
20140080226 | FLUID TRANSPORT SYSTEM WITH GASKET - Fluid transport system, including methods and apparatus, for moving fluid. The system may include a plurality of wells each having a rim. The system also may include a gasket defining a plurality of apertures that extend through the gasket from a top side to a bottom side of the gasket. The bottom side may define a plurality of grooves, with each groove extending at least partway around an axis defined by an aperture of the plurality of apertures. The gasket may be configured to have the top side of the gasket engaged with a pump assembly and at least a portion of the rim of each of the wells disposed in at least one groove of the plurality of grooves, to provide sealed communication between the pump assembly and each of the wells. | 03-20-2014 |
20140073532 | MULTIPLEX IMMUNOASSAYS FOR HEMOGLOBIN, HEMOGLOBIN VARIANTS, AND GLYCATED FORMS - Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided. | 03-13-2014 |
20140067277 | SNP DETECTION BY MELT CURVE CLUSTERING - Systems, methods and apparatus for an automated analysis of a collection of melt curves is provided. The analysis can identify certain characteristics of double stranded nucleotide sequences (e.g. DNA or other nucleotide sequences) which were melted. For example, a variation (e.g. a mutation) in the sequences (also called amplicons) may be determined from the analysis. The amplicons may be amplified via any amplification mechanism, such as PCR or Ligase chain reaction (LCR). The automated analysis can include identifying a melt region, normalizing a melt curve, and clustering melt curves. | 03-06-2014 |
20140065634 | CHIMERIC ANTI-dsDNA/CHROMATIN ANTIBODY - Provided herein are antibodies for determining the concentration of anti-dsDNA and anti-chromatin antibodies in biological samples. | 03-06-2014 |
20140057293 | CONJUGATES OF 1,4,7-TRIAZACYCLONONANES, DINUCLEAR METAL COMPLEXES OF SUCH CONJUGATES, AND METHODS OF USE FOR BOTH 1,4,7-TRIAZACYCLONONANES AND CONJUGATES - Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement. | 02-27-2014 |
20140051595 | Methods and Compositions for Determining Nucleic Acid Degradation - Described herein are methods, systems, compositions and kits to enable determination of a level of degradation of nucleic acids in a sample. For example, the determination of the amount of degradation of RNA in a sample can be accomplished by labeling one or both of the intact 5′- and/or 3′-ends of an mRNA molecule by taking advantage of the unique diol moiety present at these structures. Labeled nucleotides can then be partitioned into droplets and the amount of degradation can be determined by detecting label present in the partitions and making quantitative or qualitative comparisons with a reference sample. In some cases, the degree of degradation is also determined by factoring in the total concentration or quantity of RNA in the sample. | 02-20-2014 |
20140042027 | MODIFIED ELECTRODE BUFFERS FOR STAIN-FREE PROTEIN DETECTION IN ELECTROPHORESIS - Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products. | 02-13-2014 |
20140038237 | NUCLEIC ACID MODIFYING ENZYMES - This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid. | 02-06-2014 |
20140032126 | MULTI-STAGE, REGRESSION-BASED PCR ANALYSIS SYSTEM - Systems and methods are provided for analyzing data to determine properties of a PCR processor other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used. | 01-30-2014 |
20140027284 | TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS - Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency. | 01-30-2014 |
20140024107 | COMPACT AUTOMATED CELL COUNTER - Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm | 01-23-2014 |
20140024023 | DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING - System, including methods and apparatus, for forming droplets of an emulsion. The system may include a channel junction at which a stream of sample fluid is divided into droplets by a dividing flow of carrier fluid. The system also may include one or more features configured to position sample fluid for reduced contact between the sample fluid and one or more surface regions of the channel junction, which may improve the consistency of droplet formation. In exemplary embodiments, sample fluid may be positioned by a step member produced by an increase in channel depth, and/or by directing flow of carrier fluid to form a barrier layer between sample fluid and a wall region, such as a ceiling region or floor region, of a channel network. | 01-23-2014 |
20130344623 | NON-SCANNING SPR SYSTEM - A system for measuring an evanescent wave phenomenon at total internal reflection, the system comprising:
| 12-26-2013 |
20130343149 | FLUID MIXING AND RINSING SYSTEM FOR A FLOW CYTOMETER - Disclosed is a system that can mix deionized water and concentrated sheath fluid to provide sheath fluid in a flow cytometer system having a desired concentration. Flow rates are low, which substantially match the flow rate of sheath fluid through the nozzle, so that turbulence and air bubbles are not formed in the sheath fluid. The available deionized water is then used for back flushing and removal of sample cells and deposited salts from the sheath fluid. | 12-26-2013 |
20130340795 | TWO STATION SAMPLE AND WASHING SYSTEM - Disclosed is a two station system for a flow cytometer that includes a sample station and a wash station. During washing, the user has access to the sample station to insert a new sample. This increases the efficiency of the workflow process. Rotary clamps are used to automatically clamp the sample station and wash station to the system. A low volume pressurized cavity is used to bring the pressure of the sample to a desired pressure, which further increases productivity of the system. A transparent body is provided in the sample station so a user can view the sample during the sampling process. A backwash process is used to clean the sample injection tube and the sample uptake tube. In addition, the wash station is designed to rinse the outer surface of the sample uptake tube. | 12-26-2013 |
20130330838 | SURFACE PLASMON RESONANCE SENSOR - An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen. | 12-12-2013 |
20130323812 | IN SITU RESTORATION OF APATITE-BASED CHROMATOGRAPHY RESINS - Methods and compositions are provided for treatment of an apatite-based resin from which retained solutes have been eluted by an elution buffer that contains an alkali metal salt with solutions of calcium ion, phosphate ion, and hydroxide separately from any sample loading and elution buffers. The treatment solutions restore the resin, reversing the deterioration that is caused by the alkali metal salt in the elution buffer. | 12-05-2013 |
20130303746 | BUFFER FOR ONE-STEP DNA EXTRACTION - DNA is extracted from epithelial cells and other cells without cell walls, by use of a DNA extraction buffer that contains a C | 11-14-2013 |
20130274125 | MULTIPLEX IMMUNOASSAY FOR RHEUMATOID ARTHRITIS AND OTHER AUTOIMMUNE DISEASES - Rheumatoid arthritis and other autoimmune diseases are diagnosed by multiplex assays for antibodies to a panel of antigens that includes cyclic citrullinated peptide and at least five members of a list that includes BRAF1 506-525, BRAF2 656-675, Vimentin (protein) citrullinated, Vimentin 415-433 cit cyclic, Vimentin 58-77 cit3 cyclic, Clusterin 231-250 cit sm1 cyclic, Fibrinogen A 556-575 cit sm cyclic, Fibrinogen A 616-635 cit sm cyclic, Histones2A H2A/a 1-20 cit sm2 cyclic, Filaggrin 48-65 cit2v1 cyclic, BRAF (catalytic domain from v raf murine sarcoma viral oncogene homologue B1, amino acids 416-766). | 10-17-2013 |
20130270287 | PARTICLE DISPENSING APPARATUS AND METHOD - Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means. | 10-17-2013 |
20130267690 | REMOVAL OF VIRUCIDAL AGENTS FROM BIOMOLECULE PREPARATIONS - Methods, compositions and kits for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by hydroxyapatite (HT) or fluorapatite (FT) that is treated with a polycationic agent. In some embodiments, the antibodies are treated with a polycationic agent that is also a virucidal agent prior to purification. | 10-10-2013 |
20130252852 | Oligonucleotides Related to Lipid Membrane Attachment - A method of forming a lipid membrane attached linker comprises contacting a lipid membrane with an oligonucleotide having a first strand and a second strand of nucleic acid and two or more hydrophobic anchoring moieties located in its terminal ends. The two strands are hybridized to each other in a duplex section in a manner that the first strand terminal end is not a part of the duplex section and free from a hydrophobic anchoring moiety and the two or more hydrophobic anchoring moieties are covalently attached to the adjacent terminal ends of the first strand and the second strand of said oligonucleotide, thereby accomplishing a direct attachment of the oligonucleotide by the moieties on the same membrane. | 09-26-2013 |
20130243283 | IMAGE ACQUISITION FOR CHEMILUMINESCENT SAMPLES - An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence. | 09-19-2013 |
20130237692 | ANIONIC EXCHANGE-HYDROPHOBIC MIXED MODE - Anion exchange-hydrophobic mixed mode ligands and methods of their use are provided. | 09-12-2013 |
20130231462 | ANTI-IMMUNE COMPLEX ANTIBODIES - Provided are methods and compositions for immunoassays with improved sensitivity and specificity. The presently disclosed anti-immune complex antibodies stabilize the interaction between a primary and secondary antibody, thereby allowing for more stringent wash conditions, less background, and stronger signal. | 09-05-2013 |
20130228501 | PREPARATIVE CHROMATOGRAPHY COLUMN - A chromatography column design and a packing device, as well as a method for obtaining a column with a very compact and uniform bed are provided. The bed remains compact and uniform during operation of the column and during sanitization, storage and transportation of the column. The amount of hardware, particularly the hardware parts in contact with product, is minimized, especially with the implementation of an internal liner. With this internal liner, the packed bed can be separated from the column in intact form, or even prepared separately from, and outside of, the column. | 09-05-2013 |
20130223056 | UNIFORM EPI-ILLUMINATION OF PLANAR SAMPLES - A planar sample, particularly of the type used in biological laboratories for detection and sometimes analysis of two-dimensional arrays of proteins, nucleic acids, or other biological species, is illuminated by epi-illumination using optically filtered line lights that are arranged along opposing parallel sides of a rectangle in which the sample array resides, with two coaxial line lights on each side of the rectangle, and the two on any given side being separated by a gap whose optimal width depends on the wavelength band transmitted by the optical filter. Surprisingly, the gap eliminates the peak in intensity at the center of the sample area and the decrease that occurs from the center outward that would otherwise occur with a single continuous filtered line light, producing instead a substantially uniform intensity along the direction parallel to the line lights. | 08-29-2013 |
20130221281 | CUSTOMIZED QUALITY CONTROLS FOR ANALYTICAL ASSAYS - Solid beads each containing a selected quantity of analyte are combined and a liquid base matrix that contains attributes of a biological fluid that is to be assayed, together constitute a kit from which a laboratory technician can, at the point of use, prepare a liquid control for a particular analyte, and preferably a series of such controls at different levels of the same analyte customized for a particular assay. | 08-29-2013 |
20130220830 | ELECTRONIC CONTROL OF PH AND IONIC STRENGTH - Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided. | 08-29-2013 |
20130218352 | MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM - Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable in interchangeable manner at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform recognizes the modules and their locations, coordinates fluid connections between the modules, and provides a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. | 08-22-2013 |
20130216454 | SYSTEM AND METHOD FOR MULTI-ANALYTE DETECTION - The present invention provides a system and method for the simultaneous detection of multiple analytes in a sample. The detection system includes a housing that holds a reagent carousel rotatably coupled thereto. Further included in the housing is an incubator carousel rotatably coupled thereto. The housing also includes magnetic material that is associated with the incubation carousel for assisting in separation beads from reagent and wash solution. A robot, associated with the housing is configured to manipulate at least either the reagent carousel or the incubator carousel and transfer materials therebetween. Reaction vessels hold samples and reaction vessels handlers move the reaction vessels. Sample analysis is determined by at least one laser based detector. | 08-22-2013 |
20130210164 | REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY - Provided are methods and compositions for reducing or eliminating pH excursions in cation and anion exchange chromatography. | 08-15-2013 |
20130203172 | SELF-CONTAINED MULTI-REAGENT ASSAY DEVICE - A single closed and compartmentalized vessel is designed to contain all assay reagents necessary to conduct an assay of a liquid sample. The vessel is constructed in parts that are rotatable relative to each other, and processing steps of the assay such as combining, mixing, separating, and measuring are all conducted by either rotating the parts relative to each other or rotating the vessel as a whole, all without opening the receptacle or otherwise exposing the user to any of the liquids or substances within the receptacle. | 08-08-2013 |
20130203122 | Reduced Inhibition of One-Step RT-PCR - The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule. | 08-08-2013 |
20130203119 | NUCLEIC ACID MODIFYING ENZYMES - This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid. | 08-08-2013 |
20130196448 | SURFACE PLASMON RESONANCE SENSOR - An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen. | 08-01-2013 |
20130196350 | SENSITIVITY AND SPECIFICITY FOR OVARIAN CANCER - Improved ovarian cancer assays are provided. | 08-01-2013 |
20130190195 | NUCLEIC ACID DETECTION BY BRIDGE AMPLIFICATION ON CODED PARTICLES - Particles that generate electromagnetic spectra upon appropriate illumination and in which the spectra can be converted to codes that reflect differences in the spectra are functionalized with a plurality of identical primer pairs on which bridge amplification can be performed. Using these particles, nucleic acids in a biological sample are amplified and exposed to a label that sends a signal only when bound to double-stranded DNA. The code for each particle is correlated with the primer pairs, and the captured nucleic acids are thus detected and identified. The procedure is useful for multiplex DNA or RNA detection as well as counting of target DNA and RNA molecules in the sample. | 07-25-2013 |
20130189700 | BREAKAGE OF AN EMULSION CONTAINING NUCLEIC ACID - Methods of processing an emulsion of aqueous droplets containing nucleic acid. The methods may include breakage of the emulsion with a destabilizing fluid including a halogen-substituted hydrocarbon. | 07-25-2013 |
20130164860 | AFFINITY METHODS AND COMPOSITIONS EMPLOYING ELECTRONIC CONTROL OF PH - Methods and apparatuses for detection of target molecules are provided. | 06-27-2013 |
20130153419 | GENERATION OF ENGINEERED MOLECULAR WEIGHT STANDARDS - The invention provides methods of generating physical analog polypeptides, and polypeptides generated employing the methods of the invention, that have the same amino acid composition of a reference protein, but have an unrelated primary sequence. The invention further provides compositions and methods employing such proteins. | 06-20-2013 |
20130143300 | NOVEL COMPOSITIONS WITH POLYMERASE ACTIVITY - The invention provides novel compositions with polymerase activity and methods of using the compositions. | 06-06-2013 |
20130143272 | THERMAL CYCLER WITH VAPOR CHAMBER FOR RAPID TEMPERATURE CHANGES - Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate. | 06-06-2013 |
20130140182 | PROTEIN FRACTIONATION BASED ON pI - Methods and devices for detecting and collection analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided. | 06-06-2013 |
20130132897 | CHROMATOGRAPHY CONFIGURATION INTERFACE - Systems and methods that facilitate the setup and visualization of a configurable process, for example a chromatography experiment to be conducted using a configurable chromatography system. A user interface displays category regions representing sections of a process, and displays a plurality of selectable items representing components appropriate for placement in the category regions. A user interface control causes selection of one of the selectable items and placement of the selected item into one of the category regions, to define a selected process configuration. The user interface control may be a scrolling control that causes a set of selectable items appropriate for placement in the respective category region to scroll through the category region. When the computer displaying the user interface is also connected to the system being configured, the user interface may also facilitate setup, monitoring, and control of the resulting process. | 05-23-2013 |
20130126356 | SIMULTANEOUS PURIFICATION OF CELL COMPONENTS - Methods and devices for purification of different cell components from the same sample are provided. | 05-23-2013 |
20130122552 | LOW-MASS SAMPLE BLOCK WITH RAPID RESPONSE TO TEMPERATURE CHANGE - A sample block for use in the polymerase chain reaction, DNA sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells. | 05-16-2013 |
20130109807 | REMOVAL OF VIRUCIDAL AGENTS IN MIXED MODE CHROMATOGRAPHY | 05-02-2013 |
20130102761 | SOLID PHASE FOR MIXED-MODE CHROMATOGRAPHIC PURIFICATION OF PROTEINS - Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix. | 04-25-2013 |
20130080373 | SYSTEMS AND METHODS FOR BIOCHEMICAL DATA ANALYSIS - Methods and systems for biochemical data analysis are provided. A dataset can be received and a selection of a compare field can be used for creation of sub-groups of data to run statistical analysis on. The sub-groups of the dataset can be created based on the selection of the compare field. Statistical information about each sub-group of data can be calculated and displayed on a user display. Other information can be provided for further dataset refinements. A user may supply a control group selection, and such a selection may then result in an indication on the display of which population represents the control group. A user may supply information for further dataset filtering. Such information may be used to filter data, prior to creating the sub-groups for statistical analysis. | 03-28-2013 |
20130065249 | SIGNAL AMPLIFICATION FOR IMMUNOASSAYS BY USE OF AVIDIN-BIOTIN LINKAGES - In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites. | 03-14-2013 |
20130059754 | DIGITAL ASSAYS WITH REDUCED MEASUREMENT UNCERTAINTY - The present disclosure provides a digital assay system, includes methods and apparatus, with reduced measurement uncertainty. In an exemplary method, an expected value for a measure that is a function of a level of a first target and a level of a second target in a sample may be provided. An optimal concentration for the first target may be obtained based on the expected value. An experimental value for the measure may be determined from a digital assay with partitions formed according to the optimal concentration. | 03-07-2013 |
20130056909 | DIMENSIONAL STABILIZATION OF SLAB GEL CASSETTES TO PREVENT DISTORTION CAUSED BY SWELLING GELS - Slab gel cassettes for use in electrophoretic analyses of biologically-derived mixtures are equipped with features that prevent the plastic walls of the cassettes from bowing outward when gels retained within the cassettes swell, as gels typically do during casting, storage, or both. In certain embodiments, these features are incorporated into, or mate with, the comb-shaped inserts that form the sample wells as the gels are being cast. In other embodiments, these features are complementary features incorporated into both the comb and the cassette. | 03-07-2013 |
20130050782 | LINE SCAN CYTOMETRY SYSTEMS AND METHODS - Systems and methods for performing cytometry using a linear light sensor. An illumination field, a line scanned by the linear light sensor, or both are swept across a cell to be imaged. Relative motion between the cell and the swept illumination may be created using a movable optical component or components, by adhering cells to a plate and transporting the plate or by other techniques. | 02-28-2013 |
20130045875 | LIBRARY CHARACTERIZATION BY DIGITAL ASSAY - Methods of characterizing a nucleic acid library by digital assay. | 02-21-2013 |
20130045471 | TRAINING SYSTEM FOR INVESTIGATIONS OF BIOENGINEERED PROTEINS - Education and training in the procedures and materials for cell growth and protein expression are provided by an integrated system of modules, each fully equipped with reagents, for the entire span of procedures beginning with cell plating and inoculation and extending through protein expression, growth, purification, and assay, plus an instruction manual that sets forth all of the procedures, and in certain cases an assessment module. The system is supplied with bacterial cells already transformed for expression of proteins that are selected for their known expression procedures and properties, and is adaptable to any known protein. | 02-21-2013 |
20130040841 | DIGITAL ASSAYS WITH MULTIPLEXED DETECTION OF TWO OR MORE TARGETS IN THE SAME OPTICAL CHANNEL - System, including methods and apparatus, for performing a digital assay with multiplexed detection of two or more distinct targets in the same optical channel. | 02-14-2013 |
20130018593 | MULTI-STAGE, REGRESSION-BASED PCR ANALYSIS SYSTEM - Systems and methods are provided for analyzing data to determine properties of a PCR process or other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used. | 01-17-2013 |
20130017551 | COMPUTATION OF REAL-WORLD ERROR USING META-ANALYSIS OF REPLICATES - A system, including methods and apparatus, for performing a digital assay on a number of sample-containing replicates, each containing a plurality of sample-containing droplets, and measuring the concentration of target in the sample. Statistical meta-analysis techniques may be applied to reduce the effective variance of the measured target concentration. | 01-17-2013 |
20130005042 | HYBRID SINGLE MOLECULE IMAGING SORTER - A system for characterizing and sorting individual molecules, for example chromatin or DNA molecules. In some configurations, the system can immobilize molecules of interest suspended in fluid in a sample stage for characterization, and selectively release the immobilized molecules to different output reservoirs, depending on the characterization result. For example, fluorescent markers may be hybridized onto molecules, which may then be attached to dielectric beads and immobilized using holographic optical tweezers or other means. The system may provide elongational flow to elongate molecules, and may detect the presence and spacing of multiple fluorescent markers hybridized to the molecules. Using advanced techniques, the system may be able to characterize features with a resolution better than the theoretical resolution of the system optics. The system may utilize a microfluidic cartridge. | 01-03-2013 |
20120330866 | SYSTEM AND METHOD FOR DETERMINING AN OPTIMUM QC STRATEGY FOR IMMEDIATE RELEASE RESULTS - The present invention proposes a method for optimizing a quality control strategy for rapid release results. An embodiment of the invention includes generating a set of candidate quality control rules and for each candidate rule, computing a maximum number of patient specimens that can be tested between quality control events while keeping the expected number of correctable unacceptable results below a predetermined correctable maximum and keeping the expected number of final unacceptable results below a predetermined final maximum. Furthermore a quality control utilization rate can be computed based on the number of patient specimens tested between each quality control event and the number of reference samples tested at each quality control event. The candidate rule for which the best quality control utilization rate may be selected along with the corresponding number of patients to be tested between each quality control as the optimum quality control strategy. | 12-27-2012 |
20120329664 | MULTIPLEXED DIGITAL ASSAYS WITH COMBINATORIAL USE OF SIGNALS - System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals. | 12-27-2012 |
20120329126 | HYBRID POLYMERASES HAVING THE ABILITY TO PRODUCE LONG AMPLICONS - The present invention provides DNA polymerases having increased efficiency of amplification of long amplicons. The present invention also provides for methods of amplifying target nucleic acid molecules with the DNA polymerases for increasing the efficiency of amplification of long amplicons. | 12-27-2012 |
20120316074 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS - Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes. | 12-13-2012 |
20120314092 | CELL COUNTING SYSTEMS AND METHODS - An automated system captures multiple digital images of a sample, from different viewing areas of the sample. The images are analyzed and a test result may be reported based on the count of cells in all of the digital images. The system may be especially applicable to the testing of samples having low cell concentrations, as the variability between tests may be reduced as compared with a system that bases its test results on only a single image of the sample. The system may count the cells in a first digital image of the sample, and may capture or analyze one or more additional digital images only when it is recognized that additional measurements are needed to ensure adequate accuracy and repeatability of the test. | 12-13-2012 |
20120309644 | FLUIDICS DEVICE - The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample. | 12-06-2012 |
20120295798 | SORTING OF ADHERENT CELLS BY SELECTIVE TRANSFORMATION OF LABELS - Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed. | 11-22-2012 |
20120295300 | VIABILITY CELL COUNTING BY DIFFERENTIAL LIGHT ABSORPTION - Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided. | 11-22-2012 |
20120295270 | METHODS OF USING IMPROVED POLYMERASES - This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase. | 11-22-2012 |
20120276623 | DNA REMOVAL IN TARGET MOLECULE PURIFICATION - Removal of genomic DNA from biological samples using cation exchange is described. | 11-01-2012 |
20120264915 | CHROMATOGRAPHY PURIFICATION OF ANTIBODIES - Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step. | 10-18-2012 |
20120264652 | DIRECTED HETEROBIFUNCTIONAL LINKERS - A heterobifunctional linker that comprises (a) a moiety capable of reversibly coupling the linker to a selected epitope of a target molecule; and (b) a moiety or moieties capable of irreversibly coupling the linker to the target, and optionally a moiety or moieties capable of irreversibly coupling the linker to a solid surface. Also described is a method of orienting molecules, such as proteins, with respect to a surface and to each other, employing such a linker, and an array of such oriented molecules, which may be the same or different. | 10-18-2012 |
20120258500 | PCR REACTION MIXTURES WITH DECREASED NON-SPECIFIC ACTIVITY - The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification. | 10-11-2012 |
20120258460 | Sso7-Polymerase Conjugates with Decreased Non-Specific Activity - Improved Sso7-polymerase conjugate proteins are provided | 10-11-2012 |
20120253723 | SYSTEM AND METHOD FOR PRODUCING STATISTICALLY VALID ASSAY MEANS AND RANGES FOR QUALITY CONTROL MATERIALS - A method of establishing statistically valid assay means and ranges for quality control materials, used to qualify medical testing machines, utilizes tests on a new lot of quality control material to establish an assay mean, and uses data from a database of historical test results to establish an assay range. The system may estimate the variability of test results from prior lot data, and then compute the limits of the assay range such that a new test on a new lot of the quality control material will be expected to fall within the range with a specified probability. Because historical data is used to estimate the test variability, the number of new tests required to specify a statistically valid mean and range may be dramatically reduced, as compared with establishing the mean and range based only on tests of the new lot of material. | 10-04-2012 |
20120252098 | Dissociation of Product-complexed Contaminants in Chromatography - The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule. | 10-04-2012 |
20120225467 | MODIFIED DNASE COMPOSITIONS AND METHODS OF USE THEREOF - Modified DNase polypeptides and methods of their use are provided. | 09-06-2012 |
20120220491 | RETENTATE CHROMATOGRAPHY AND PROTEIN CHIP ARRAYS WITH APPLICATIONS IN BIOLOGY AND MEDICINE - Analytes in a sample are resolved by retentate chromatography in a procedure involving adsorbing the analytes on a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery. | 08-30-2012 |
20120211679 | CYTOMETRY SYSTEM WITH SOLID NUMERICAL-APERTURE-INCREASING LENS - A flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. The flow element includes a bore bounded by a wall. A light source is configured to illuminate the cell. An optical system receives light emanating from the cell and directs at least some of the received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of the emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens. | 08-23-2012 |
20120208193 | DETECTING METHYLATION IN A SUBPOPULATION OF GENOMIC DNA - This invention provides methods of determining the biological, pathological, genetic, epigenetic or disease status in a biological sample by determining the methylation status of a subpopulation of genomic DNA in the sample. | 08-16-2012 |
20120202975 | ELUTION OF PROTEINS FROM HYDROXYAPATITE RESINS WITHOUT RESIN DETERIORATION - Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin. | 08-09-2012 |
20120192901 | APATITE SURFACE NEUTRALIZATION WITH ALKALI SOLUTIONS - The present invention discloses methods of neutralizing apatite surfaces, for example after a flow-through collection of a target and prior to cleaning the chromatography solid support. | 08-02-2012 |
20120164690 | UNIVERSAL REFERENCE DYE FOR QUANTITATIVE AMPLIFICATION - Universal reference dye mixtures for quantitative amplification, and uses thereof, are provided. | 06-28-2012 |
20120138099 | WASH RING ASSEMBLY AND METHOD OF USE - Devices, including a wash ring assembly, and methods are provided for the removal of excess fluid or solids from the exterior or interior of a probe used to transfer fluids, for instance, in an automated assay device. Typically, a probe is used to aspirate and dispense a sample fluid material such as whole blood or a reagent. The devices and methods provided herein are useful for removing excess fluid from the exterior or interior of the probe so as to prevent dripping and cross-contamination between samples or reagents. It is also contemplated that, utilizing the devices and methods provided herein, washing and/or drying can be performed simultaneously as the probe is in motion, aspirating a sample and/or dispensing a sample. | 06-07-2012 |
20120129238 | REVERSE TRANSCRIPTASE MIXTURES WITH IMPROVED STORAGE STABILITY - Reverse transcriptase mixtures with improved storage stability are provided. | 05-24-2012 |
20120125440 | ROTARY COLUMN SELECTOR VALVE - A rotary valve is designed to include a stator with multiple pairs of ports for connection to flow-through components and a rotor with elongated recesses that form flow channels with minimal dead volume and that permit a reversal of flow direction through any component by rotation of the valve through a very small angle. | 05-24-2012 |
20120124810 | PISTON MOVEMENT CONTROL FOR PREPARATIVE CHROMATOGRAPHY COLUMN - In an axial-flow preparative chromatography column that contains a piston, commonly termed an adaptor, that is lowered over the top of the resin, the ability to lower the piston in a controlled manner to minimize the chances of damage to the resin is achieved by use of an aperture either in the piston head itself or in a compound bolt that secures the upper column frit to the piston head. The aperture is closed with a removable plug during storage and use of the piston head and column. During packing of the bed, however, the plug is replaced by a graduated rod operating as a dipstick to allow the operator to determine the distance between the bottom face of the piston head and the upper surface of the column packing. | 05-24-2012 |
20120122158 | PCR FOR DNA THAT IS RESISTANT TO AMPLIFICATION - DNA that is difficult to amplify by conventional PCR is amplified by a modified PCR process that includes a high-temperature, short-term heating step as the last step of each thermal cycle, following the conventional denaturing and annealing/elongation steps. | 05-17-2012 |
20120113428 | SURFACE PLASMON RESONANCE SENSOR - An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen. | 05-10-2012 |
20120103074 | Automated Analyzer with Low-Pressure In-Line Filtration - An automated analyzer for biological samples that contain particulate matter is adapted to be able to process a large number of samples without changing the analytical cartridge, and in many cases without changing the internal switching valves, by including a filter in the low-pressure section of the analyzer, and preferably configuring the analyzer to be able to perform backflushing on the filter between sample injections. | 05-03-2012 |
20120090999 | POLYMERIC SORBENT SHEETS AS ION RESERVOIRS FOR ELECTROBLOTTING - Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred. | 04-19-2012 |
20120084010 | Dose Surface Method for Determination of Analyte Ratios - Methods, systems, and apparatus for accurately determining a proportion (ratio) of two analytes is provided, as well as provide a concentration of a first analyte from a determined concentration of a second analyte and from a proportion of the analytes to each other. In one aspect, a surface model (called a “dose surface” herein) relating the concentrations of the two analytes to the proportion can be used to obtain accurate values for one of the variables (e.g. a concentration or the proportion) when the other two variables have previously been obtained. The dose surface can be a three-dimensional surface and be non-linear. The dose surface model can include multiple regression functions. For example, measured responses can be individually converted to concentrations using two dose-response curves, and the concentrations can be input to a dose surface function to obtain the proportion. | 04-05-2012 |
20120082991 | DETECTION OF RNA-INTERACTING REGIONS IN DNA - The present invention provides methods and kits for detecting RNA-interacting regions in genomic DNA. | 04-05-2012 |
20120077961 | ENHANCED PURIFICATION OF ANTIBODIES AND ANTIBODY FRAGMENTS BY APATITE CHROMATOGRAPHY - Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed. | 03-29-2012 |
20120070829 | SIZE SELECTION OF DNA FOR CHROMATIN ANALYSIS - Methods for analyzing chromosomal DNA, including chromatin, are provided. | 03-22-2012 |
20120064517 | DETECTION OF CHROMATIN STRUCTURE - The present invention provides methods of determining the accessibility of genomic DNA to a DNA modifying agent. | 03-15-2012 |
20120058565 | CONJUGATES OF 1,4,7-TRIAZACYCLONONANES, DINUCLEAR METAL COMPLEXES OF SUCH CONJUGATES, AND METHODS OF USE FOR BOTH 1,4,7-TRIAZACYCLONONANES AND CONJUGATES - Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement. | 03-08-2012 |
20120055555 | DIAPHRAGM VALVE - Novel features enhancing the operation of a diaphragm valve include a specialized diaphragm profile for enhanced securement of the diaphragm in the valve body, and a quick-connect feature joining the diaphragm to its actuator, in which the forward end of the actuator contains a sloping surface, an expanded tip, and a shoulder, while the diaphragm contains a truncated bore with a complementary profile. The valve can be modular in construction to permit mounting of the valve to an instrument panel in close proximity to other valves or components to provide the ability to select among interconnected fluid flow paths. | 03-08-2012 |
20120046765 | SYSTEM AND METHOD FOR ULTRASONIC TRANSDUCER CONTROL - A system for controlling an ultrasonic transducer utilizes an indication of the relative phase of the voltage on the transducer and the current passing through the transducer to control the relative phase to a value that has been preselected to correspond to resonance of an electromechanical system that includes the ultrasonic transducer. In one example application, the system includes a waveform generator that produces an oscillating signal, and the frequency of the oscillating signal is adjustable under control of the control processor. The control processor adjusts the frequency of the oscillating signal according to a proportional control law at least some of the time, and according to a proportional-integral control law at least some of the time. The control processor may also control the amplitude of the oscillation of the ultrasonic transducer. | 02-23-2012 |
20120031759 | DIELECTROPHORETIC DEVICE WITH ACTUATOR - A dielectrophoretic fractionation device having a channel defining a direction of flow therethrough for the fractionation of particles in a liquid particle suspension is disclosed. The device also has a deflector unit affixed to a wall of the channel and arranged to generate an electric field gradient in the channel to spatially separate the particles in the liquid particle suspension. The device also has an actuator mounted to the channel and positioned to deform the wall. Also provided is a method for inhibiting aggregation of particles in a liquid particle suspension of the dielectrophoretic fractionation device, the method including periodically deforming the wall with the actuator. | 02-09-2012 |
20120015392 | Cell Counting Slide With Lateral Reservoir For Promoting Uniform Cell Distribution - Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber. | 01-19-2012 |
20120013727 | CELL CHARACTERIZATION USING MULTIPLE FOCUS PLANES - A system for characterizing cells takes a series of digital images of a sample containing the cells. Each of the images is taken at a different plane of focus. One of the images is determined to have been taken at a plane of best focus. The system analyzes the digital image taken at the plane of best focus and at least one other of the digital images to classify cells in the sample as either live or dead. | 01-19-2012 |
20110306042 | DETERMINATION OF CHROMATIN CONFORMATION - Methods and kits for determining histone modification or epigenetic status are provided. | 12-15-2011 |
20110297545 | INSTRUMENT FOR INDEPENDENT ELECTROTRANSFER IN MULTIPLE CASSETTES - Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes. | 12-08-2011 |
20110297544 | ELECTROTRANSFER CASSETTE WITH INTEGRATED ELECTRICAL CONTACTS AND LOCKING MECHANISM - An electrotransfer cassette is formed in two parts that are releasably joined by a manually operated locking mechanism. The joined parts have electrical contact areas extending from the electrodes in the two parts of the cassette, the contact areas being exposed on an outer edge of the resulting cassette to form electrical connections to a power supply upon the simple insertion of the cassette into an instrument. | 12-08-2011 |
20110270974 | SYSTEM AND METHOD FOR PROVIDING AUTOMATICALLY UPDATED PRODUCT INSERTS - Systems, apparatuses, and methods can provide parameters of operating results for control products used in biological reactions. For example, automatically updated inserts containing such parameters for clinical quality controls can be provided. A customer can log into a website, provide lot number of quality control products, information about instruments, and tests being performed and then receive updated parameters for the products. The product inserts can be customized for or by a particular customer. | 11-03-2011 |
20110267622 | SURFACE PLASMON RESONANCE SENSOR - An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen. | 11-03-2011 |
20110263823 | ENHANCED CAPACITY AND PURIFICATION OF PROTEIN BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications. | 10-27-2011 |
20110250167 | METHOD FOR PURIFYING PROTEINS - The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample. | 10-13-2011 |
20110222068 | NEAR REAL TIME OPTICAL PHASE CONJUGATION - An optical system and associated method enable near real time optical phase conjugation. In the method, a translucent medium is illuminated by a sample illumination beam. Light scattered by the medium is directed to an electronic image sensor while a reference beam is also directed to the electronic image sensor. The scattered light and the reference beam form an interference pattern at the electronic image sensor. A digital representation of the interference pattern is recorded using the electronic image sensor, and the characteristics of a conjugate of the sample beam are computed from the numerical representation. A conjugate beam having the computed characteristics is generated using a configurable optical element and directed back to the translucent medium. The generation of the conjugate beam may be accomplished using a spatial light modulator. | 09-15-2011 |
20110222051 | MICROASSEMBLED IMAGING FLOW CYTOMETER - A microassembled imaging cytometer includes a sensing location that undergoes relative motion with a cell. Light from a light source is focused by a focusing element to a plurality of focused illumination spots or lines at the sensing location, illuminating the cell as the cell traverses the sensing location. A collection lens collects light emanating from the cell and refocuses the collected light onto an array light sensor. The focusing element may include an array of microlenses having spherical or aspheric surfaces. The system may include a processing unit that constructs a digital image of the cell based at least in part on signals produced by the array light sensor indicating the intensity and distribution of light falling on the array light sensor. The system may characterize cells using light emanating from the cells by fluorescence. | 09-15-2011 |
20110211058 | COMPACT AUTOMATED CELL COUNTER - Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm | 09-01-2011 |
20110204256 | HIGH-SPEED CELLULAR CROSS SECTIONAL IMAGING - A cross sectional imaging system performs high-resolution, high-speed partial imaging of cells. Such a system may provide much of the information available from full imaging cytometry, but can be performed much more quickly, in part because the data analysis is greatly reduced in comparison with full image cytometry. The system includes a light source and a lens that focuses light from the light source onto a small spot in a scanning location. A transport mechanism causes relative motion between a cell in the scanning location and the spot. A sensor generates a signal indicating the intensity of light emanating from the cell as a result of illumination by the light source. The system repeatedly takes readings of the light intensity signal and characterizes the light intensity along a substantially linear path across the cell. | 08-25-2011 |
20110190169 | SIMULTANEOUS ASSAY FOR DETERMINING DRUGS - Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies. In an alternative procedure, the added drugs are not coupled to the microparticles but are pre-labeled, while the antibodies are pre-coupled to the microparticles, and the assay proceeds without further incubation. In both alternatives, the microparticles are ultimately recovered from the assay medium and from any unbound species, and the recovered microparticles are analyzed by flow cytometry to obtain indications of the presence of the various drugs in the sample in an inverse manner by detection of the label, each drug differentiable from the others by the distinguishing features of the microparticles. | 08-04-2011 |
20110177599 | DELIVERY OF NUCLEIC ACIDS ACROSS MEMBRANES - The present invention provides for methods and compositions for introducing integral membrane proteins into cell membranes and, optionally, delivery of nucleic acids across membranes via the integral membrane proteins. | 07-21-2011 |
20110165654 | NUCLEIC ACID MODIFYING ENZYMES - This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid. | 07-07-2011 |
20110139718 | PISTON MOVEMENT CONTROL FOR PREPARATIVE CHROMATOGRAPHY COLUMN - In an axial-flow preparative chromatography column that contains a piston, commonly termed an adaptor, that is lowered over the top of the resin, the ability to lower the piston in a controlled manner to minimize the chances of damage to the resin is achieved by use of an aperture either in the piston head itself or in a compound bolt that secures the upper column frit to the piston head. The aperture is closed with a removable plug during storage and use of the piston head and column. During packing of the bed, however, the plug is replaced by a graduated rod operating as a dipstick to allow the operator to determine the distance between the bottom face of the piston head and the upper surface of the column packing. | 06-16-2011 |
20110139689 | Monitoring A Preparative Chromatography Column From the Exterior During Formation of the Packed Bed - In an axial-flow cylindrical preparative chromatography column that utilizes a piston to close off the top of the resin space inside the column and thereby eliminate void spaces at the top of the resin, a sight glass is incorporated in the column wall to allow monitoring of the packing height and density as the packing material is being placed inside the column, as well as the position of the piston head relative to the packing while the piston is being lowered to consolidated and/or compress the packing. By observing the column interior through the sight glass, the operator can assure that the column receives a more uniform packing density and can minimize the risk of damage to the packing material by excessive force from the piston head. | 06-16-2011 |
20110136250 | BEAD READER - A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising:
| 06-09-2011 |
20110134238 | CALIBRATION OF IMAGING DEVICE FOR BIOLOGICAL/CHEMICAL SAMPLES - Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size. | 06-09-2011 |
20110130560 | SONICATION CARTRIDGE FOR NUCLEIC ACID EXTRACTION - A cartridge in which sonication is applied to biological matter to disrupt and release nucleic acids from the matter is formed from a cartridge body containing a series of wells connected by fluid passages engineered to prevent backflow, with at least one well containing a sonication window covered by a thin lamina to transmit sonic vibrations from a sonication horn contacting the exterior surface of the window. Fluid transport among the wells is achieved by pressure differentials through the fluid passages, and a succession of functions is performed in the various wells, including disruption, mixing, binding of the released nucleic acids to binding materials, washing, elution, and collection. | 06-02-2011 |
20110117670 | MULTIPLEX IMMUNOASSAYS FOR HEMOGLOBIN, HEMOGLOBIN VARIANTS, AND GLYCATED FORMS - Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided. | 05-19-2011 |
20110111978 | DIVERSE CHEMICAL LIBRARIES BOUND TO SMALL PARTICLES WITH PARAMAGNETIC PROPERTIES - The present invention provides diverse chemical libraries bound to small particle with paramagnetic properties. Typically, the chemical structures comprise a plurality of different chemical moieties, the particles are paramagnetic and have a diameter between about 100 nm and about 10 microns, the chemical structures bound to each particular particle have substantially the same structure and the combinatorial library comprises at least 100,000 different chemical structures. | 05-12-2011 |
20110104760 | METHODS AND COMPOSITIONS FOR IMPROVING EFFICIENCY OF NUCLEIC ACIDS AMPLIFICATION REACTIONS - The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced. | 05-05-2011 |
20110104686 | RAPID DETECTION OF MYCOPLASMA CONTAMINATION IN CELL CULTURE SAMPLES - The present invention provides for methods of detecting | 05-05-2011 |
20110009287 | PURIFICATION OF MONOCLONAL ANTIBODIES - Methods and compositions for efficiently purifying monoclonal antibodies and identifying pairs of antibodies compatible in sandwich immunoassays are provided. | 01-13-2011 |
20100328766 | MODULAR MICROSCOPE CONSTRUCTION - A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination. | 12-30-2010 |
20100305777 | PISTON POSITION DETECTION FOR PREPARATIVE CHROMATOGRAPHY COLUMN - In an axial-flow cylindrical preparative chromatography column that utilizes a piston to close off the top of the resin space inside the column and thereby eliminate void spaces at the top of the resin, one or more proximity detectors are incorporated in the piston head to either generate a signal indicating the proximity of the piston had to the resin, or to collect a signal, such as a optical signal, emitted from the resin. The value of the signal or any change in the signal is compared to a threshold value to ascertain when the piston head is in proximity to the resin so that the motion of the piston toward the resin can be halted before contact pressure from the piston head damages the resin. | 12-02-2010 |
20100305310 | Multi-Media Affinity Column to Prevent Leaching of Ligands - In an affinity-type purification, ligands dissociated from a packed bed that would otherwise leach into the solution containing the species being purified are captured by a second ligand that resides in a porous barrier downstream from the packed bed, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column. | 12-02-2010 |
20100280228 | PURIFICATION OF IMMUNOCONJUGATES - The present invention provides methods of purifying immunoconjugates. | 11-04-2010 |
20100279360 | Sso7-Polymerase Conjugate Proteins - This invention provides Sso7-polymerase conjugates that exhibit improved activity in a polymerase reaction. | 11-04-2010 |
20100267163 | NON-SCANNING SPR SYSTEM - A system for measuring an evanescent wave phenomenon at total internal reflection, the system comprising:
| 10-21-2010 |
20100250145 | SNP DETECTION BY MELT CURVE CLUSTERING - Systems, methods and apparatus for an automated analysis of a collection of melt curves is provided. The analysis can identify certain characteristics of double stranded nucleotide sequences (e.g. DNA or other nucleotide sequences) which were melted. For example, a variation (e.g. a mutation) in the sequences (also called amplicons) may be determined from the analysis. The amplicons may be amplified via any amplification mechanism, such as PCR or Ligase chain reaction (LCR). The automated analysis can include identifying a melt region, normalizing a melt curve, and clustering melt curves. | 09-30-2010 |
20100243452 | ISOELECTRIC FOCUSING TRAY AND ELECTRODE ASSEMBLY FOR ALTERNATE GEL STRIP ORIENTATIONS - The procedures involved in isoelectric focusing that entail the use of an electric current can be performed with the gel side of the IPG strip facing either up or down in a tray and electrode assembly in which the electrodes are mounted on an insert from which the inner ends of the electrodes extend into the troughs to reside either below the IPG strips or above them. Slots in one part (either the insert or the tray) mate with walls or partitions in the other part to secure the two parts together, and the heights of the electrodes within the troughs of the tray can be adjusted by the height of the insert in the tray or by a resilient mounting of the electrode to the insert. | 09-30-2010 |
20100238442 | SERIAL-LINE-SCAN-ENCODED MULTI-COLOR FLUORESCENCE MICROSCOPY AND IMAGING FLOW CYTOMETRY - A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics. | 09-23-2010 |
20100220330 | OPTICAL RESONANCE ANALYSIS USING A MULTI- ANGLE SOURCE OF ILLUMINATION - An SPR or other optical resonance based analysis system in which, light is provided at multiple angles to a specimen and then the light modified by the specimen is processed to select only some of the light. Optionally, the processing selects light at a particular incidence angle. Optionally, the detection is by imaging of the light on a 2D imager array. | 09-02-2010 |
20100213064 | ELECTROBLOTTING CASSETTE WITH MANUALLY RELEASABLE ELECTRODES OF ADJUSTABLE SPACING - An electroblotting cassette is formed in three separable parts—an upper plate, a lower plate, and a base that receives both plates, with electrodes mounted on both the upper and lower plates. The cassette accommodates transfer stacks of different thicknesses by its inclusion of a set of raised areas, known as “lands,” on the floor of the base and a set of inverse lands on the underside of the lower electrode plate, the two sets being spatially arranged to either abut each other or be offset from each other, depending on the orientation of the lower plate, thereby allowing the user a choice between two heights of the lower plate within the base and hence two thicknesses of transfer stacks. Other arrangements include those with more than one set of lands on one or both parts to allow for three or more thickness selections, or depressions in place of lands. Finger-operated latches secure the upper plate to the base. | 08-26-2010 |
20100213060 | AQUEOUS TRANSFER BUFFER - The present invention relates to an aqueous transfer buffer that provides superior efficiency in transferring polypeptides of a broad range of molecular weight from a matrix used in electrophoresis to another immobilized surface. Also disclosed are electrophoretic methods and devices in which the aqueous transfer solution of this invention is used. | 08-26-2010 |
20100193441 | Chromatography Column With Pack, Unpack, and Clean-In-Place Features - A preparative chromatography column is equipped with one or more valves that can each be used to both inject a column packing into the column or withdraw used packing from the column, in addition to cleaning the supply and discharge lines associated with the valve itself, all by moving a movable plug within the valve body and all without protruding into the column interior. Each valve is mounted on the lateral wall of the column just above the filter plate at the column floor, and the plug is movable between a closed position in which the end surface of the plug is flush with the inner wall of the column and an open position in which the plug is recessed within the bore. Each valve has two additional ports in its valve body, positioned such that when the plug is closed, both ports are open to the valve bore, and when the plug is open, at least one of the ports is open to the bore. The position of the plug within the valve bore determines the valve function. | 08-05-2010 |
20100187441 | TRANSILLUMINATOR ADAPTOR FOR CONVERSION OF ULTRAVIOLET RADIATION TO VISIBLE LIGHT - An adaptor is designed as an accessory to an ultraviolet transilluminator for the excitation of fluorescent molecules or labels in a planar array of biochemical samples such as a two-dimensional electrophoresis gel to enable the emissions resulting from the excitation to be detected and quantified. The adaptor is constructed to overlay the transilluminator and contains both a fluorescent dye that upon excitation by ultraviolet light emits light in the visible spectrum, and a conditioning substance that selects a portion of the wavelength band of the visible light produced by the fluorescent dye. The adaptor converts the ultraviolet light from the transilluminator to visible light while limiting the emissions reaching the detector to those that emanate from the sample. By the use of this adaptor, the transilluminator is adapted for use with samples labeled with dyes that are excitable by visible light and avoids exposure of the samples and the user to ultraviolet light. | 07-29-2010 |
20100187114 | HIGH-PERFORMING ELECTROPHORESIS GELS WITH EXTENDED SHELF LIVES - Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components. | 07-29-2010 |
20100184616 | SPATIALLY CONTROLLED ILLUMINATION OF BIOLOGICAL SAMPLE ARRAY THROUGH WEDGE-SHAPED SUPPORT - Two-dimensional samples or sample arrays such as electrophoresis gels and microplates, containing fluorescently labeled species, are illuminated by an illumination device that includes a slab of non-autofluorescing or low-autofluorescing material shaped to receive excitation light from one or more edges and to distribute the light to emerge from an upper surface of the slab at a uniform intensity along the length and width of the upper surface. | 07-22-2010 |
20100178647 | SIMULATION OF NORMAL FRESH BLOOD PLATELETS - The present invention provides for novel analogs of fresh human platelets for use in hematological instrument. Methods for preparing such analogs are also described. | 07-15-2010 |
20100173791 | PROCESS DESIGN USING MASS SPECTROMETRY - The present invention provides a facile and efficient method for determining a chromatographic protocol for separating a target protein from one or more second protein impurity. Also provided is a database facilitating the determination of an appropriate separation protocol. | 07-08-2010 |
20100159447 | QUANTITATIVE AMPLIFICATION WITH A LABELED PROBE AND 3' TO 5' EXONUCLEASE ACTIVITY - This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe. | 06-24-2010 |
20100145029 | ENHANCED CAPACITY AND PURIFICATION OF ANTIBODIES BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications. | 06-10-2010 |
20100144046 | CYCLOMETALATED TRANSITION METAL COMPLEXES FOR MULTIPLEX ANALYTE DETECTION - A complex containing a transition metal ion and a plurality of donor ligands each of which is fully coordinated to the transition metal ion and is either a nitrogen donor ligand or a cyclometalated donor ligand, such that at least one of the donor ligands is a cyclometalated donor ligand bears one or more reactive groups connected to at least one of the donor ligands through a linker that includes a chain of four or more atoms. The linker offers advantages that make the complex particularly effective in labeling biomolecules and in multiplex analyses. | 06-10-2010 |
20100140174 | SUPPORTING STRUCTURES FOR PREPARATIVE CHROMATOGRAPHY COLUMNS - The plunger used as the upper enclosure in a preparative chromatography column is suspended by one or more rods that are supported by a frame through coupling connections on the frame that can fix the position of the rod(s) relative to the frame, while the position of the rod(s) is controlled by a motorized drive system that is suspended above the column. The drive system is mounted either to the column lid or to a functional plate positioned a short distance above the column lid. These constructions allow the plunger to be raised and lowered so that the column and its parts can be exchanged, all without the need for a hoist or crane. | 06-10-2010 |
20100136559 | CHROMATIN STRUCTURE DETECTION - The present application provides methods and compositions for determining accessibility of a DNA modifying agent in genomic DNA. | 06-03-2010 |
20100133165 | SUPPORTING STRUCTURES FOR PREPARATIVE CHROMATOGRAPHY COLUMNS - The plunger used as the upper enclosure in a preparative chromatography column is suspended by one or more rods that are supported by a frame through coupling connections on the frame that can fix the position of the rod(s) relative to the frame, while the position of the rod(s) is controlled by a motorized drive system that is suspended above the column. The drive system is mounted either to the column lid or to a functional plate positioned a short distance above the column lid. These constructions allow the plunger to be raised and lowered so that the column and its parts can be exchanged, all without the need for a hoist or crane. | 06-03-2010 |
20100125128 | PHOSPHOPEPTIDE ENRICHMENT OF COMPOSITIONS BY FRACTIONATION ON CERAMIC HYDROXYAPATITE - Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput. | 05-20-2010 |
20100099581 | LOCALIZED TEMPERATURE CONTROL FOR SPATIAL ARRAYS OF REACTION MEDIA - Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels. | 04-22-2010 |