Patent application title: NOVEL PRRS VIRUS INDUCING TYPE I INTERFERON IN SUSCEPTIBLE CELLS
Inventors:
Andreas Gallei (Hannover, DE)
IPC8 Class: AC12N700FI
USPC Class:
4242041
Class name: Drug, bio-affecting and body treating compositions antigen, epitope, or other immunospecific immunoeffector (e.g., immunospecific vaccine, immunospecific stimulator of cell-mediated immunity, immunospecific tolerogen, immunospecific immunosuppressor, etc.) virus or component thereof
Publication date: 2016-02-11
Patent application number: 20160040136
Abstract:
The present invention relates to the field of attenuated live viruses
useful as vaccine or medicament for preventing or treating Porcine
Reproductive and Respiratory Syndrome (PRRS) in swine, and is based on
the surprising finding of a PRRS virus which is able to induce the
interferon type I response of a cell infected by said virus. In one
embodiment, the PRRS virus according to the invention is a PRRS virus
mutant comprising, in comparison with the genome of a wild type strain, a
mutation in the gene encoding the non structural protein 1 (nsp1) of said
virus.Claims:
1. A replication-competent Porcine Reproductive and Respiratory Syndrome
(PRRS) virus comprising a deletion of at least three consecutive
nucleotide triplets in the gene sequence encoding the N-terminal domain
(NTD) of the nsp1.beta. subunit of the nsp1 protein of said virus,
wherein the NTD starts with the N-terminal amino acid sequence SXXY (SEQ
ID NO: 60) of nsp1.beta. and ends with the serine (S) residue within the
amino acid sequence SFP (SEQ ID NO: 62), wherein said deletion of at
least three consecutive nucleotide triplets comprises a deletion of the
nucleotide triplet encoding the first arginine residue (R) located at
least 21 amino acid residues in the C-terminal direction from the
N-terminal amino acid residue of nsp1.beta. and a deletion of the two
nucleotide triplets coding for the two amino acid residues flanking said
R residue in the N-terminal direction, and wherein the modified PRRS
virus induces production and secretion of interferon type I by a cell
infected with said virus.
2. The PRRS virus according to claim 1, wherein said mutation comprises a deletion of three consecutive nucleotide triplets coding for the amino acid sequence RXR (SEQ ID NO: 66) of the nsp1.beta. subunit, or wherein said mutation comprises a deletion of four consecutive nucleotide triplets coding for the amino acid residues GRXR (SEQ ID NO: 68) of the nsp1.beta. subunit, wherein X is a genetically encoded amino acid residue.
3. An immunogenic composition comprising the PRRS virus according to claim 1.
4. The immunogenic composition according to claim 3, comprising an amount of 10.sup.1 to 10.sup.7 viral particles per dose
5. The immunogenic composition according to claim 3, comprising an amount of 10.sup.3 to 10.sup.6 particles per dose.
6. The immunogenic composition according to claim 3, comprising an amount of 10.sup.4 to 10.sup.6 particles per dose.
7. The immunogenic composition according to claim 3, comprising an amount of said PRRS virus which is equivalent to a virus titer of at least about 10.sup.3 TCID50/mL per dose.
8. The immunogenic composition according to claim 3, comprising an amount of said PRRS virus which is equivalent to a virus titer of between 10.sup.3 to 10.sup.5 TCID50/mL per dose.
9. The immunogenic composition according to claim 3, further comprising one or more pharmaceutically acceptable carriers or excipients.
10. The immunogenic composition according to claim 9, wherein said one or more pharmaceutically acceptable carriers or excipients are selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.
11. The PRRS virus according to claim 1, wherein said PPRS virus has increased sensitivity to interferon type I.
12. The PRRS virus according to claim 1, wherein said PRRS virus is an attenuated PRRS virus.
13. The PRRS virus according to claim 1, wherein said PPRS virus is a genotype I virus.
14. The PRRS virus according to claim 1, wherein said interferon type I is Interferon-.alpha., and/or Interferon-.beta. or combinations thereof.
15. The PRRS virus according to claim 1, wherein said cell is a mammalian cell, and wherein said mammalian cell is a cell of a primary or secondary cell line or a host cell.
16. The PRRS virus according to claim 15, wherein said mammalian cell is a porcine cell or a simian cell.
17. The PRRS virus according to claim 16, wherein said porcine cell is a porcine macrophage.
18. The PRRS virus according to claim 16, wherein said simian cell is a MA-104 or a MARC-145 cell.
19. A polynucleotide comprising the genome of the PRRS virus according to claim 1.
20. A DNA Vector comprising a copy of the polynucleotide of claim 19.
21. A virus particle comprising the polynucleotide of claim 19.
22. A cell comprising the polynucleotide of claim 19.
23. A cell comprising the DNA-vector of claim 20.
24. A method of preparing a PRRS virus composition comprising the cultivation of the PRRS virus according to claim 1 in cell culture.
25. A method for the treatment of Porcine Reproductive and Respiratory Syndrome comprising administering an effective amount of the PRRS virus according to claim 1 to a swine, pig, piglet or a sow in need of said treatment.
26. A method for inducing an immune response against PRRSV comprising administering an effective amount of the immunogenic composition according to claim 1 to a swine, pig, piglet or a sow in need thereof.
27. A method for the detection of interferon type I and/or the mRNA coding for interferon type I, by the cell infected with the modified PRRS virus according to claim 1, wherein the interferon type I and/or the mRNA coding for the interferon type I is detected by a bioassay, wherein said bioassay is preferably selected from the group consisting of ELISA, PCR, GLISA, IFA, biosensoric measurement, Surface Plasmon Resonance (SPR) measurement, selective media, lateral flow, biochip measurement, immunomagnetic separation, electrochemiluminescence, chromogenic media, immunodiffusion, DNA hybridization, staining, colorimetric detection, luminescence, and combinations thereof.
28. The method according to claim 24, wherein the immunogenic composition is administered in a single dose or in two doses.
Description:
SEQUENCE LISTING
[0001] The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Oct. 1, 2014 is named 01-2738-SEQ-US-2.txt and is 243,257 bytes in size.
FIELD OF THE INVENTION
[0002] The present invention belongs to the field of vaccines and medicaments for the prophylaxis and treatment of infectious diseases. In particular, it relates to attenuated live viruses useful as vaccine or medicament for preventing or treating Porcine Reproductive and Respiratory Syndrome (PRRS), a viral disease affecting swine.
BACKGROUND OF THE INVENTION
[0003] Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the virus family Arteriviridae and belongs, together with the Coronaviridae, to the virus order Nidovirales. PRRSV is an enveloped virus with a single-stranded, positive-sense RNA genome of about 15 kilobases comprising nine open reading frames (ORFs), namely ORF1a, ORF1ab, ORF2a, ORF 2ab, and ORFs 3 through ORF7. ORFs1a and 1ab encode large polyproteins that are processed into the viral nonstructural proteins (nsp) by auto- and transcleavages of viral proteases nsp1, nsp2, and nsp4 (Snijder and Meulenberg, 1998).
[0004] There are two distinct viral PRRSV genotypes causing similar clinical symptoms that diverge by about 40% on nucleotide sequence level, genotype I (EU) and genotype II (US). The North American (US) prototype strain is VR-2332, while the European (EU) prototype strain is Lelystad virus.
[0005] PRRSV is considered one of the economically most important infectious agents in pigs causing late-term reproductive failure in sows and respiratory disease in growing pigs. Often, PRRSV infection is complicated by secondary bacterial infections being attributed to the immunosuppressive nature of the virus. Also, PRRSV viremia lasts for weeks, and virus then still can be detected in lymphoid organs for several months, demonstrating difficulties or failure of the host's immune response to clear the virus (Allende et al., 2000).
[0006] The specific immune response to PRRSV infection is characterized by delayed induction of neutralizing antibodies (Lopez and Osorio, 2004) and short cell-mediated immune response (Xiao et al., 2004). It is commonly accepted that these effects can in part be attributed, along with presentation of decoy epitopes (Ostrowski et al., 2002; Ansari et al., 2006) and glycan shielding of viral envelope proteins (Ansari et al., 2006), to the viral inhibition of the host's innate immune system. It has been demonstrated that PRRSV infection does not or only weakly or delayedly induce production of type I interferon (IFN) (interferon α and interferon β; (Miller et al., 2004)) or type II IFN, (interferon α; (Meier et al., 2003)) in susceptible cell lines (swine pulmonary alveolar macrophages, monkey kidney cells MARC-145) and/or pigs (Buddaert et al., 1998).
[0007] Thus, there is a need for novel vaccines and medications effecting a rapid induction of neutralizing antibodies and interferon responses for the prophylaxis and treatment of PRRSV infection.
BRIEF DESCRIPTION OF THE FIGURES
[0008] FIG. 1: Sequence alignment of nsp1β protein sequences from parental EU PRRSV strain LoN94-13 and from vaccine candidates.
[0009] FIG. 2: Sequence alignment of nsp1β proteins from PRRSV strains.
[0010] FIG. 3A: PRRSV-specific immunofluorescence of virus stock titrations of delta nsp1 XVII-1.Black, negative fluorescence; light grey, few positive cells; grey, foci of positive cells; dark grey, complete cell monolayer positive.
[0011] FIG. 3B: PRRSV-specific immunofluorescence of virus stock titrations of delta nsp1 XVIII-12. Black, negative fluorescence; light grey, few positive cells; grey, foci of positive cells; dark grey, complete cell monolayer positive.
[0012] FIG. 4: Virus stocks of vaccine candidates contain IFNβ.
[0013] FIG. 5: Time course of IFNβ induction after infection of MA104 cells.
[0014] FIG. 6: Delta nsp1 mutants show increased sensitivity to type I IFN.
[0015] FIG. 7: Mean body temperatures of vaccinated groups before and after challenge.
DETAILED DESCRIPTION
[0016] IFNs play an important role in establishing an effective adaptive immune response against viral infections, and many viruses therefore have developed strategies and counteractions against onset of the host's innate immune system (Haller and Weber, 2009). In the interest to identify the anticipated PRRSV IFN antagonist(s), extensive screening analyses based on cell lines stably expressing genes of interest or on cells transfected with protein-expressing plasmids have identified several PRRSV nonstructural proteins (nsps) including nsp1 (see below), nsp2 (Beura et al., 2010; Li et al., 2010), nsp4 (Beura et al., 2010), and nsp11 (Beura et al., 2010; Shi et al., 2011a) to be involved in blocking the induction of type I IFN.
[0017] nsp1 is located at the N-terminus of the PRRSV ORF1a-derived polyprotein 1a and is processed into two multifunctional subunits, nsp1α and nsp1β, each of which contains a papain-like cystein protease (PCP) domain essential for self-release from the viral polyprotein (den Boon et al., 1995; Chen et al., 2010). nsp1α contains an N-terminal zinc-finger domain and the PCPα protease domain, while nsp1β contains PCPβ. For both nsp1 subunits, nsp1α and nsp1β, the three-dimensional crystal structure has been resolved (Sun et al., 2009; Xue et al., 2010). According to these analyses, nsp1β consists of an N-terminal domain (NTD), a linker domain (LKD), the PCP domain (PCP beta), and a C-terminal extension (CTE); (Xue et al., 2010), see FIG. 2. C-terminal, nsp1β-mediated cleavage of nsp1 from nsp2 occurs at site WYG/AGR for PRRSV US strains (Kroese et al., 2008) or is predicted at site WYG/AAG for PRRSV EU strains (Chen et al., 2010), while nsp1α/nsp1β cleavage occurs at site ECAM/AxVYD for PRRSV US strains or is predicted at site EEAH/SxVYR for PRRSV EU strains (Chen et al., 2010).
[0018] Several studies on protein level demonstrated to the mechanistic detail that PRRSV nsp1 and/or its autocleavage-derived subunits nsp1α and/or nsp1β inhibit type I IFN production by interfering with IFN transcription (Song et al., 2010; Kim et al., 2010; Chen et al., 2010; Beura et al., 2010). In addition, it has been demonstrated that nsp1β interferes with the cellular response to interferon (interferon signaling); (Chen et al., 2010). Moreover, it was demonstrated that PRRSV infection inhibits IFNα and/or IFNβ production in PRRSV infected cells in vitro (Kim et al., 2010; Beura et al., 2010), the subcellular localization of nsp1(subunits) was determined (Song et al., 2010; Chen et al., 2010), and mechanistic aspects of type I IFN inhibition that were obtained by others from single protein expression experiments were confirmed in cells infected with PRRSV (Shi et al., 2010). Finally, a nsp1 mutagenesis study based on nsp1 protein expression investigated effects on viral IFN inhibition (Shi et al., 2011b), showing that mutations that inactivated papain-like cysteine protease activity of nsp1α made nsp1 lose its IFN antagonism activity, whereas mutations that inactivated papain-like cysteine protease activity of nsp1β did not influence the IFN antagonism activity of nsp1.
[0019] However, a viable PRRSV strain that induces IFN production and/or does not interfere with IFN signaling after infection of susceptible cells and/or the host, in particular a PRRSV strain comprising a genomic mutation in its nsp1 gene, has not been described yet.
[0020] It is thus an aim of the present invention to make available such a viable PRRS virus inducing the IFN response of a cell, in particular of a host cell, wherein said PRRS virus may serve as an effective vaccine or medicament for the prophylaxis or treatment of the Porcine reproductive and respiratory syndrome in swine.
[0021] The solution to the above technical problem is achieved by the description and the embodiments characterized in the claims.
[0022] Thus, the invention in its different aspects and embodiments is implemented according to the claims.
[0023] The invention is based on the surprising finding of a Porcine Reproductive and Respiratory Syndrome (PRRS) virus which is able to induce the interferon type I response of a cell, in particular of a host cell.
[0024] Hence, one aspect of the invention concerns a PRRS virus (PRRSV) which is able to induce the interferon type I production and secretion by a cell infected by said virus, wherein the PRRS virus according to the invention in particular is able to induce or induces the interferon type I production and secretion by an interferon competent cell infected by said virus.
[0025] As used herein, it is understood that the terms "interferon type I", "IFN type I", "type I interferon" and "type I IFN" are equivalent.
[0026] Interferon type I production and secretion by a cell, as described herein, particularly means that interferon type I is made and released by a cell in response to the infection of the PRRS virus according to the invention. In this regard, interferon type I, as mentioned herein, is preferably interferon-α and/or interferon-β.
[0027] The infection of a cell by the PRRS virus according to the invention in particular includes attachment of the virus to the cell, entry of the virus into the cell, disassembly of the virion, and preferably replication and transcription of the viral genome, expression of viral proteins, and assembly and release of new infectious viral particles.
[0028] The cell, as mentioned herein, is a primary or secondary susceptible cell, preferably a mammalian cell, in particular a porcine or a simian cell, more preferably said cell is a porcine macrophage or a MA-104 cell or a MARC-145 cell or a Vero cell.
[0029] It is further understood that the PRRS virus according to the invention is able to induce in vitro and/or in vivo the interferon type I production and secretion by a cell infected by said virus.
[0030] Preferably, the PRRS virus according to the invention is a live PRRS virus and/or a modified-live PRRS virus and/or an attenuated PRRS virus.
[0031] The term "attenuated PRRS virus", as described herein, is in particular directed to a PRRS virus which is attenuated in vitro and/or in vivo, more particular in susceptible cell lines and/or the host.
[0032] The term "host", as used herein, is in particular directed to animals infectable with PRRS virus, in particular swine, more particular pigs, such as domestic pigs.
[0033] As mentioned herein, "attenuated" particularly relates to a reduced virulence of a pathogen, in particular of a wild type PRRS virus, wherein "virulence" is understood to be the degree of pathogenicity, and wherein "pathogenicity" is directed to the ability of the pathogen to produce clinical symptoms in the host, such as elevated body temperature.
[0034] In particular preferably, the PRRS virus according to the invention has or shows increased sensitivity to type I INF when compared to wild type PRRSV, wherein the term "sensitivity to type I INF" is understood as reduced viral infectivity when IFNβ is present, preferably in a sufficient amount for significantly reducing viral infectivity of wild type PRRSV, in the medium surrounding the virus at the time of infection.
[0035] The term "wild type PRRS virus" or "wild type PRRSV", respectively, as used herein, is in particular directed to an infectious pathogenic PRRS virus, which is particularly capable of causing PRRS in swine. In one particular preferred embodiment, the term "wild type PRRS virus" is directed to a PRRS virus whose genome comprises a RNA sequence or consists of a RNA polynucleotide, wherein said RNA sequence or RNA polynucleotide is a RNA copy of a polynucleotide selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.
[0036] In one embodiment, the PRRS virus according to the invention is a PRRS virus mutant, in particular comprising, in comparison with the genome of a wild type PRRSV strain, a mutation in a gene encoding a protein of said virus.
[0037] In a preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene encoding the nsp1 protein of said virus. Thus, the invention preferably concerns a PRRS virus which is able to induce the interferon type I production and secretion by an infected cell as a result of a mutation in the gene encoding the nsp1 protein, wherein said mutation is preferably a mutation as mentioned hereinafter.
[0038] Hence, the invention particularly concerns a PRRS virus comprising, in comparison with the genome of a wild type strain, a mutation in the gene encoding the nsp1 protein. Accordingly, the mutation as described herein is preferably a mutation in comparison with the sequence of the corresponding wild type gene encoding the nsp1 protein.
[0039] The invention is thus preferably directed to a PRRS virus; in particular a wild type PRRS virus, wherein a mutation, preferably a mutation as mentioned hereinafter, has been implemented in the genome of said virus resulting in a non-natural nsp1 protein or in the lack of nsp1 protein of said virus.
[0040] In the context of the invention it is understood, that the mutation as described herein may be implemented to any PRRS virus, such as to a PRRS virus selected from the group consisting of wild type PRRS virus, attenuated PRRS virus, modified-life PRRS virus, PRRS virus mutant and combinations thereof. In one example, the mutation as described herein may be implemented to an attenuated and/or live PRRS virus, for example to a PRRS virus selected from the group of the PRRS virus strains that have been deposited on 27 Oct. 2004 with the European Collection of Cell Cultures (ECACC), Porton Down, Salisbury, Wiltshire, SP4 OJG, Great Britain, under the Accession Numbers ECACC 04102703, ECACC 04102702, and ECACC 04102704. Hence, the mutation as described herein can be combined with one or more other mutations, preferably one ore more other attenuating mutations, in a PRRS virus.
[0041] In the context of the invention, the term "PPRRS virus" is in particular equivalent with "PRRS virus strain".
[0042] The term "mutation" in the context of the invention is understood as a change in a genomic sequence, in particular in the RNA sequence of a PRRS virus. Since viruses that use RNA as their genetic material have rapid mutation rates, the term "mutation", as mentioned herein, is particularly directed to a genetically engineered change in a genomic sequence, such as by site directed mutagenesis, which in particular results in a virus growing to titers significantly lower than wild type PRRS virus in interferon competent cells and/or in the infected host, when propagated under the same conditions. Moreover, in another preferred embodiment the mutation described herein can also be caused by natural mutation and subsequent isolation of the PRRS virus according to the invention, wherein said isolated virus includes the mutation described herein.
[0043] Preferably, the mutation, as described herein, comprises or consists of one or more point mutations and/or one or more genomic deletions and/or one or more insertions.
[0044] The term "nsp1 protein", as used herein, is directed to the PRRSV nonstructural protein 1.
[0045] The nsp1 protein is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1 protein is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:3 if the PRRS virus according to the invention is a genotype II PRRS virus.
[0046] In one aspect, the PRRS virus according to the invention preferably also may comprise a mutation in the gene encoding the nsp1 protein selected from the group consisting of SEQ ID NOs 1-3.
[0047] It is hence understood that the PRRS virus according to the invention is a genotype I PRRS virus or a genotype II PRRS virus. It is further understood that the terms "genotype I" and "genotype II" are equivalent to the terms "genotype 1" and "genotype 2" or to the terms "type 1" and "type 2", as frequently used in the literature in the context of PRRSV.
[0048] Sequence identity in the context of the invention is understood as being based on pairwise determined similarity between nucleotide or protein sequences. The determination of percent identity between two sequences is preferably accomplished using a mathematical algorithm, in particular the well-known Smith-Waterman algorithm (Smith and Waterman, M. S. (1981) J Mol Biol, 147(1):195-197). For purposes of the present invention, percent sequence identity of an amino acid sequence is determined using the Smith-Waterman homology search algorithm using an affine 6 gap search with a gap open penalty of 12 and a gap extension penalty of 2, BLOSUM matrix 62. The Smith-Waterman homology search algorithm is taught in Smith and Waterman (1981) Adv. Appl. Math 2:482-489, herein incorporated by reference. A variant may, for example, differ from the reference nsp1, nsp1α, nsp1β or NTD molecule by as few as 1 to 15 amino acid residues, as few as 1 to 10 amino acid residues, such as 6-10, as few as 5, as few as 4, 3, 2, or even 1 amino acid residue. Alternatively, percent identity of a nucleotide sequence is determined using the Smith-Waterman homology search algorithm using a gap open penalty of 25 and a gap extension penalty of 5. Such a determination of sequence identity can be performed using, for example, the DeCypher Hardware Accelerator from TimeLogic Version G, or the sequence identity is determined with the software CLC MAIN WORKBENCH 4.1.1 (CLC BIO).
[0049] The term "having 100% sequence identity", as used herein, is understood to be equivalent to the term "being identical".
[0050] In another preferred embodiment, the mutation in the PRRS virus according to the invention comprises or consists of one or more point mutations and/or one or more genomic deletions and/or one or more insertions.
[0051] In a further preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene sequences encoding the nsp1α subunit of the nsp1 protein and/or the nsp1β subunit of the nsp1 protein of said virus.
[0052] The term "nsp1α", as mentioned herein, is thus directed to the PRRSV nsp1α subunit of the nsp1 protein, and the term "nsp1β", as used herein, is hence directed to the PRRSV nsp1β subunit of the nsp1 protein.
[0053] The nsp1α is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:4 and SEQ ID NO:5 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1α is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:6 if the PRRS virus according to the invention is a genotype II PRRS virus.
[0054] The nsp1β is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1β is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:9 if the PRRS virus according to the invention is a genotype II PRRS virus.
[0055] In a particular preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene sequence encoding the N-terminal domain (NTD) of the nsp1β of said virus. The term "NTD", as mentioned herein, is thus directed to the N-terminal domain of the PRRSV nsp1β subunit.
[0056] The NTD is preferably a polypeptide or has a polypeptide sequence, respectively, having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide or a polypeptide sequence, respectively, selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11 if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD is preferably a polypeptide or has polypeptide sequence, respectively, having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide or the polypeptide sequence, respectively, set forth in SEQ ID NO:12 if the PRRS virus according to the invention is a genotype II PRRS virus.
[0057] In a preferred embodiment, the NTD comprising the mutation has the sequence selected from the group consisting of the sequences
TABLE-US-00001 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
as set forth in SEQ ID NOs:36-41, if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD comprising the mutation preferably has the sequence selected from the group consisting of the sequences
TABLE-US-00002 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS,
as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to the invention is a genotype II PRRS virus.
[0058] Thus, the PRRS virus according to the invention preferably comprises a gene sequence encoding a NTD selected from the sequences
TABLE-US-00003 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
as set forth in SEQ ID NOs:36-41, if the PRRS virus according to the invention is a genotype I PRRS virus, or the PRRS virus according to the invention preferably comprises a gene sequence encoding a NTD selected from the sequences
TABLE-US-00004 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS,
as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to the invention is a genotype II PRRS virus.
[0059] In one exemplary embodiment, the NTD comprising the mutation may, for instance, have the sequence selected from the group consisting of the sequences
TABLE-US-00005 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,
as set forth in SEQ ID NOs:48-53, if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD comprising the mutation may, for instance, have the sequence selected from the group consisting of the sequences
TABLE-US-00006 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,
as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to the invention is a genotype II PRRS virus.
[0060] Thus, in one exemplary embodiment, the PRRS virus according to the invention may, for instance, comprise a gene sequence encoding a NTD selected from the sequences
TABLE-US-00007 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,
as set forth in SEQ ID NOs:48-53, if the PRRS virus according to the invention is a genotype I PRRS virus, or the PRRS virus according to the invention may, for instance, comprise a gene sequence encoding a NTD selected from the sequences
TABLE-US-00008 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,
as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to the invention is a genotype II PRRS virus.
[0061] It is thus understood that a genotype I PRRS virus, as mentioned herein, is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7 and SEQ ID NO:8, or is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11.
[0062] Preferably, the genotype I PRRS virus, as mentioned herein, comprises or consists of a RNA polynucleotide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a RNA polynucleotide complementary to a polynucleotide selected from the group consisting of SEQ ID NO: 13 and SEQ ID NO: 14.
[0063] It is further understood that a genotype II PRRS virus, as mentioned herein, is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:3, SEQ ID NO:6 and SEQ ID NO:9, or is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:12.
[0064] Preferably, the genotype II PRRS virus, as mentioned herein, comprises or consists of a RNA polynucleotide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a RNA polynucleotide complementary to the polynucleotide set forth in SEQ ID NO: 15.
[0065] In the following, the single letter code for amino acids is used for amino acid sequences, where additionally X signifies any genetically encoded amino acid residue.
[0066] Within the context of the invention it is in particular understood that the N-terminal domain (NTD) of nsp1β starts with the N-terminus of nsp1β, preferably starts with the amino acid sequence SXXY if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence AXVY if the PRRS virus is a genotype II PRRS virus, and the NTD ends with the serine residue (S) within the amino sequence SFP of the nsp1β. Regarding the NTD domain of a genotype II PRRS virus it is in particular referred to the publication Xue et al. 2010, hereby incorporated by reference. Said amino acid sequences SXXY or AXVY and SFP are especially conserved in the nsp1β of wild type PRRS viruses and are thus preferably used amino acid sequence motifs for defining the NTD domain according to the invention.
[0067] In a particular preferred embodiment, the PRRS virus according to the invention thus comprises a mutation in the gene sequence coding for the NTD, wherein the NTD preferably starts with motif SXXY for PRRS virus type 1 strains or with motif AXVY for PRRS virus type 2 strains and ends with the serine residue (S) within the conserved SFP motif of the nsp1β subunit.
[0068] According to the invention, it is in particular preferred if said mutation is located within the part of the gene sequence coding for the NTD, where the amino acid stretches folding into B sheet secondary structures are encoded.
[0069] In one preferred aspect, the mutation, as described herein, thus comprises a deletion or replacement of a nucleotide triplet coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.
[0070] In particular, it is preferred if the mutation described herein comprises of a deletion or replacement of 2 to 7 or more, preferably consecutive, nucleotide triplets each coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.
[0071] In another preferred aspect, the mutation, as mentioned herein, comprises the deletion or replacement of a nucleotide triplet coding for an amino acid residue with a charged, preferably positively charged, side chain, in particular coding for an arginine residue.
[0072] Exemplarily, the mutation mentioned herein preferably consists of a deletion of 2, 3, 4, 5, 6 or 7 consecutive nucleotide triplets coding for the respective number (2, 3, 4, 5, 6 or 7) of consecutive amino acid residues located within the first 24 N-terminal amino acid residues of the NTD sequence, wherein this mutation comprises the deletion of a nucleotide triplet coding for an amino acid residue with a charged, preferably positively charged, side chain, in particular coding for an arginine residue.
[0073] The mutation, as described herein, preferably comprises or consists of a deletion or replacement of the nucleotide triplet coding for the first arginine residue (R) located at least 21 amino acid residues in C-terminal direction from the N-terminal amino acid residue of the nsp1β NTD and/or the mutation, as mentioned herein, comprises a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the nsp1 amino acid sequence RXMW if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence RGG of said virus if the PRRS virus is a genotype II PRRS virus and/or the mutation, as mentioned herein, comprises or consists of a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the amino acid sequence RMM or RGG of the nsp1 protein.
[0074] According to the invention, the phrase "replacement of a nucleotide triplet" is understood as being a replacement of a nucleotide triplet of the wild type sequence by another nucleotide triplet coding for a different amino acid residue than the wild type sequence.
[0075] The phrase "deletion of nucleotide triplet" in the context of the invention is directed to a deletion of a nucleotide triplet resulting in the deletion of an amino acid residue in comparison with the wild type sequence.
[0076] In particular, said mutation further comprises a deletion or replacement of one nucleotide triplet coding for the amino acid residue flanking said arginine residue (R) in N-terminal direction, wherein said mutation preferably comprises or consists of a deletion or replacement of two consecutive nucleotide triplets coding for the amino acid residues PR.
[0077] More particular, said mutation further comprises a deletion or replacement of two, three, four, five, six, seven or more consecutive nucleotide triplets coding for two, three, four, five, six, seven or more amino acid residues flanking said arginine residue (R) in N-terminal direction.
[0078] Preferably, said mutation comprises a deletion or replacement of three consecutive nucleotide triplets coding for the amino acid sequence RXR or APR of the nsp1β, or wherein said mutation comprises a deletion or replacement of four consecutive nucleotide triplets coding for the amino acid residues GRXR or WAPR of the nsp1β.
[0079] As result, the PRRS virus according to the invention comprises in one embodiment a gene coding for a nsp1 protein selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19 and SEQ ID NO:20, or comprises in another embodiment a gene sequence coding for a nsp1β selected from the group consisting of SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24 and SEQ ID NO:25, or comprises in a further embodiment a gene sequence coding for a NTD selected from the group consisting of SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29 and SEQ ID NO:30.
[0080] In yet another preferred embodiment, the mutation, as described herein comprises a deletion of the gene sequence encoding the (whole) nsp1 protein, encoding the (whole) nsp1α subunit, encoding the (whole) nsp1β subunit, or encoding a part, preferably at least seven consecutive amino acid residues, of or the (whole) NTD domain of the nsp1β subunit.
[0081] Yet another aspect of the invention is directed to a polynucleotide comprising or consisting of the genome of the PPRS virus according to the invention.
[0082] Further, the invention is also directed to a virus particle, wherein said virus particle comprises a polynucleotide which comprises or consists of the genome of the PPRS virus according to the invention or which comprises or consists of a DNA copy of the PPRS virus according to the invention.
[0083] Still further, the present invention provides a DNA-Vector comprising a copy of, or a cDNA sequence complementary to, respectively, a polynucleotide which comprises or consists of the genome of the PPRS virus according to the invention.
[0084] In one exemplary and non-limiting example the DNA vector, as mentioned herein, comprises a polynucleotide sequence selected from the group consisting of SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and SEQ ID NO:35.
[0085] Also, the present invention provides a cell comprising a polynucleotide, which comprises or consists of the genome of the PPRS virus according to the invention or which comprises or consists of a DNA copy of the PPRS virus according to the invention.
[0086] In one exemplary and non-limiting example the PRRS virus according to the invention, or the genome of the PRRS virus according to the invention, respectively, comprises a RNA sequence or consists of a RNA polynucleotide, wherein said RNA sequence or RNA polynucleotide is a RNA copy of a polynucleotide selected from the group consisting of SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34 and SEQ ID NO: 35.
[0087] Another aspect of the invention concerns the PRRS virus according to the invention for use as vaccine or medicament, in particular for use in the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine. The present invention further provides a composition containing the PRRS virus according to the invention for use as a vaccine or as a medicament, in particular for use in the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine.
[0088] In a preferred aspect, said vaccine or medicament is administered in two doses or preferably in one dose to an animal in need thereof. By the term "prophylaxis or therapy", as mentioned herein, it is meant that the prophylaxis is to administer a drug, in particular a vaccine, before exposure to PRRSV and the treatment is to administer a drug, in particular a medicament, after infection with PRRSV or onset of PRRS.
[0089] In particular, the vaccine, as mentioned herein, is a live vaccine and/or a modified live vaccine and/or an attenuated live or attenuated modified live vaccine.
[0090] The strains of the PRRS virus according to the invention can be grown and harvested by methods known in the art, e.g. by propagating in suitable cells like the simian cell line MA-104, Vero cells, or porcine alveolar macrophages. Preferably, vaccines according to the present invention are modified live vaccines comprising one or more of these strains alive in a suitable carrier, but inactivated virus may also be used to prepare killed vaccine (KV). Modified live vaccines (MLV) are typically formulated to allow administration of 101 to 107 viral particles per dose, preferably 103 to 106 particles per dose, more preferably 104 to 106 particles per dose (4.0-6.0 log10 TCID50). KV may be formulated based on a pre-inactivation titre of 103 to 1010 viral particles per dose. The vaccine may comprise a pharmaceutically acceptable carrier, for example a physiological salt-solution. The vaccine may or may not comprise an adjuvant. A suitable adjuvant may optionally also be used. For example, a suitable adjuvant that may be used a-tocopherol acetate which may be obtained under the trade name DILUVAC FORTE®. Alternatively, alum based adjuvants may be used.
[0091] A further aspect of the invention is thus directed to the use of the PRRS virus according to the invention for the preparation of a vaccine or medicament for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome, preferably in swine.
[0092] A vaccine according to the present invention may be presented in form of a freeze-dried preparation of the live virus, to be reconstituted with a solvent, to result in a solution for injection. The solvent may, for example, be water, physiological saline, a buffer, or an adjuvanting solvent. The solvent may additionally use adjuvants, for example a-tocopherol acetate. The reconstituted vaccine may then be injected into a pig, for example as an intramuscular or intradermal injection into the neck. For intramuscular injection, a volume of 2 ml may be applied, for an intradermal injection it is typically 0.2 ml. In a further aspect, the present invention therefore is a product, comprising in separate containers a freeze-dried composition of the virus, and a solvent for reconstitution, and optionally further containing a leaflet or label comprising instructions of use.
[0093] Yet another aspect of the invention thus concerns a method for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome comprising administering the PRRS virus according to the invention to an animal, preferably to a swine, more preferably to a pig, in particular preferably to a piglet or a sow.
[0094] A vaccine according to the present invention may not only comprise the PRRS virus according to the invention as antigen, but may include further components active against PRRS or other porcine viral or bacterial diseases, like porcine circovirus, classical swine fever virus or mycoplasma hyorhinis. Therefore, the invention further relates to a vaccine as described, characterized in that it contains at least one further antigen active against a porcine disease which is not PRRS.
[0095] Still another aspect of the invention thus concerns a medicament or vaccine comprising the PRRS virus according to the invention.
[0096] Still a further aspect of the invention is directed to a method of preparing a vaccine or medicament, in particular for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine, said method comprising the cultivation of the PRRS virus according to the invention in cell culture.
[0097] Moreover, the invention comprises a method of producing the PRRS virus according to the invention, said method comprising the mutagenesis of the gene coding for the nsp1 protein in the genome of a PRRS virus, and wherein the mutation, as described herein, is in particular introduced by genetic engineering in the genome of said virus, preferably by site directed mutagenesis.
[0098] Preferably, for any of the aforementioned methods, the above-mentioned DNA-vector is used and/or said methods comprise the detection of interferon type I, in particular interferon type I secretion, and/or of mRNA coding for interferon type I. Said detection in particular comprises the detection of interferon type I and/or of mRNA coding for interferon type I by a bioassay, wherein said bioassay is preferably selected from the group consisting of ELISA, PCR, GLISA, IFA, biosensoric measurement, Surface Plasmon Resonance (SPR) measurement, selective media, lateral flow, biochip measurement, immunomagnetic separation, electrochemiluminescence, chromogenic media, immunodiffusion, DNA hybridization, staining, colorimetric detection, luminescence, and combinations thereof.
[0099] In yet a further aspect, the invention provides an immunogenic composition containing the PRRS virus according to the invention.
[0100] As used herein, the term "immunogenic composition" in particular refers to a composition that will elicit an immune response in an animal that has been exposed to the composition. An immune response may include induction of antibodies and/or induction of a T-cell response.
[0101] Usually, an "immune response" includes but is not limited to one or more of the following effects: the production or activation of antibodies, B cells, helper T cells, suppressor T cells, and/or cytotoxic T cells, directed specifically to an antigen or antigens included in the composition or vaccine of interest. Preferably, the host will display either a therapeutic or a protective immunological (memory) response such that resistance to new infection will be enhanced and/or the clinical severity of the disease reduced. Such protection will be demonstrated by either a reduction in number or severity of, or lack of one or more of the symptoms associated with the infection of the pathogen, in the delay of onset of viremia, in a reduced viral persistence, in a reduction of the overall viral load and/or in a reduction of viral excretion. Thus, an "immune response" in particular means but is not limited to the development in a subset of a cellular and/or antibody-mediated immune response to the composition or vaccine of interest.
[0102] In one aspect, the immunogenic composition of the invention is preferably a vaccine or medicament.
[0103] As used herein, the term "viremia" is particularly understood as a condition in which PRRS virus particles circulate in the bloodstream of an animal.
[0104] The term "animal", as mentioned herein, is in particular directed to swine, more particular to a pig, preferably a domestic pig.
[0105] In a preferred aspect, the immunogenic composition of the invention comprises an amount of 101 to 107 viral particles of the PRRS virus according to the invention per dose, preferably 103 to 106 particles per dose, more preferably 104 to 106 particles per dose.
[0106] In another preferred aspect, the immunogenic composition of the invention comprises an amount of the PRRS virus according to the invention which is equivalent to a virus titre of at least about 103 TCID50/mL per dose, preferably between 103 to 105 TCID50/mL per dose
[0107] In yet another preferred aspect, the immunogenic composition of the invention further contains one or more pharmaceutically acceptable carriers or excipients. Said one or more pharmaceutically acceptable carriers or excipients are preferably selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.
[0108] The present invention is further directed to the immunogenic composition of the invention for use in a method for
[0109] inducing an immune response against PRRSV, or
[0110] the prevention or reduction of PRRS, or
[0111] the prevention or reduction of PRRSV viremia and/or
[0112] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or
[0113] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal.
[0114] As used herein, the term "inducing an immune response" to an antigen or composition is the development of a humoral and/or cellular immune response in an animal to an antigen present in the composition of interest.
[0115] The term "prevention" or "reduction" or "preventing" or "reducing", respectively, as used herein, means, but is not limited to a process which includes the administration of a PRRSV antigen, namely of the PRRSV according to the invention which is included in the composition of the invention, to an animal, wherein said PRRSV antigen, when administered to said animal elicits or is able to elicit an immune response in said animal against PRRSV. Altogether, such treatment results in reduction of the clinical symptoms of PRRS or of symptoms associated with PRRSV infection, respectively. More specifically, the term "prevention" or "preventing, as used herein, means generally a process of prophylaxis in which an animal is exposed to the immunogenic composition of the present invention prior to the induction or onset of the disease process (PRRS).
[0116] Herein, "reduction of PRRS" or "reduction of clinical symptoms associated with PRRSV infection" means, but is not limited to, reducing the number of infected subjects in a group, reducing or eliminating the number of subjects exhibiting clinical symptoms of infection, or reducing the severity of any clinical symptoms that are present in the subjects, in comparison to wild-type infection. For example, it should refer to any reduction of pathogen load, pathogen shedding, reduction in pathogen transmission, or reduction of any clinical sign symptomatic of PRRSV infection, in particular of elevated body temperature. Preferably these clinical symptoms are reduced in subjects receiving the composition of the present invention by at least 10% in comparison to subjects not receiving the composition and may become infected. More preferably, clinical symptoms are reduced in subjects receiving the composition of the present invention by at least 20%, preferably by at least 30%, more preferably by at least 40%, and even more preferably by at least 50%.
[0117] Also, the elevated body temperature usually associated with the administration of an attenuated PRRSV vaccine to an animal is reduced in subjects receiving the composition of the present invention by at least 10% in comparison to subjects receiving a conventional attenuated PRRSV vaccine. More preferably, the elevated body temperature usually associated with the administration of an attenuated PRRSV vaccine is reduced in subjects receiving the composition of the present invention by at least 20%, preferably by at least 30%, more preferably by at least 40%, and even more preferably by at least 50%.
[0118] Hence, a further advantage of the PRRS virus according to the invention is in particular the effect that its administration to an animal, e.g. for the prophylaxis or treatment of PRRS, results in a reduced increase of body temperature in the animal in comparison with a conventional attenuated PPRSV vaccine.
[0119] The term "conventional attenuated PRRSV vaccine", as mentioned herein, is in particular directed to any attenuated PRRSV useful as a vaccine, wherein said PRRSV does not have the characteristic features of the PRRS virus of the present invention, in particular is not able to induce the interferon type I production and secretion by a cell infected by said PRRSV.
[0120] The term "subject", as mentioned herein, in particular relates to an animal.
[0121] The term "body temperature", as used herein, in particular refers to the approximate average normal, internal temperature of an animal, for example about 38.5-39° C. in pigs, whereas the body temperature associated with a PRRSV infection may be elevated up to 41° C. in pigs.
[0122] The term "reduction of PRRSV viremia" means, but is not limited to, the reduction of PRRS virus entering the bloodstream of an animal, wherein the viremia level, i.e. the number of PRRSV RNA copies per mL of blood serum or the number of plaque forming colonies per deciliter of blood serum, is reduced in the blood serum of subjects receiving the composition of the present invention by at least 50% in comparison to subjects not receiving the composition and may become infected. More preferably the viremia level is reduced in subjects receiving the composition of the present invention by at least 90%, preferably by at least 99.9%, more preferably by at least 99.99%, and even more preferably by at least 99.999%.
[0123] The present invention further relates to the use of the PRRS virus according to the invention or of the immunogenic composition of the invention for the preparation of a vaccine or medicament for
[0124] inducing an immune response against PRRSV, or
[0125] treating or preventing PRRS, or
[0126] preventing or reducing PRRSV viremia and/or
[0127] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or
[0128] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal.
[0129] In particular, it is preferred if the vaccine or medicament, as mentioned herein, is to be administered in two doses or preferably in a single dose to an animal.
[0130] The present invention also provides a method of preparing an immunogenic composition, preferably a vaccine or medicament, more preferably a vaccine or medicament for
[0131] inducing an immune response against PRRSV, or
[0132] treating or preventing PRRS, or
[0133] preventing or reducing PRRSV viremia and/or
[0134] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or
[0135] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal, wherein said method comprises the step of mixing the PRRS virus according to the invention with one or more pharmaceutically acceptable carriers or excipients, and wherein said one or more pharmaceutically acceptable carriers or excipients are preferably selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.
[0136] In another aspect, the present invention further provides a method for
[0137] inducing an immune response against PRRSV, or
[0138] treating or preventing PRRS, or
[0139] preventing or reducing PRRSV viremia and/or
[0140] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or
[0141] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal, wherein said method comprises the step of administering the immunogenic composition of the invention to an animal in need thereof, and wherein preferably the immunogenic composition and/or the vaccine is administered in two doses or, more preferably, in a single dose.
Examples
[0142] In the examples five viable, genetically designed PRRSV mutant strains are described which are based on infectious EU PRRSV cDNA clone LoN94-13. These strains, delta nsp1 IX-10, delta nsp1 XVII-1, delta nsp1 XVIII-12, delta nsp1 XIX-2 and delta nsp1 XX-9 (henceforth referred to as vaccine candidates), harbor genomic deletions of two, three, four, five, or six codons in their predicted nsp1 genes, respectively, resulting in deletions of two (motif P21R22), three (motif R20P21R22), four (motif G19R20P21R22), five (motif N18G19R20P21R22), or six (motif (P17N18G19R20P21R22) amino acids in their predicted nsp1β proteins, respectively (FIG. 1).
[0143] Based on sequence alignments of parental strain LoN94-13 with PRRSV US and EU reference strains VR-2332 and Lelystad virus as well as with strain GD-XH, the deletions are located in the predicted nsp1β portion of nsp1 (FIG. 2). In more detail, the deletion site for all vaccine candidates is located in the N-terminal domain (NTD) of nsp1β and overlaps with amino acids P23R24 of GD-XH nsp1β; (FIG. 2).
[0144] After transfection of synthetic transcripts of the vaccine candidates into BHK21 cells and transfer of cell culture supernatant from transfected BHK21 cells onto PRRSV-susceptible MA104 cells, plaque formation typical for PRRSV infection occurred. PRRSV-specificity and viability for each of the vaccine strains then was demonstrated by subsequent cell culture passages on MA104 cells and PRRSV-specific immunofluorescence using monoclonal antibody SDOW17 (Rural Technologies).
[0145] After endpoint dilution and generation of virus stocks each derived from material of a single virus plaque, virus titers of the obtained virus stocks were determined for each vaccine candidate by serial virus titrations on 96-well plates containing MA104 cells followed by PRRSV-specific immunofluorescence analyses six to seven days post infection. Unlike experience with titrations of virus stocks from parental PRRSV LoN94-13, the first serial dilutions of vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 did not demonstrate a cytopathic effect and virus plaque formation, while at higher dilutions of the virus stocks a cytopathic effect was detectable. Moreover, when respective titrations were investigated by immunofluorescence, cell culture wells of the first serial dilutions were negative for PRRSV infection for both vaccine candidates, while wells infected with higher dilutions of the virus stocks showed PRRSV-specific immunofluorescence, respectively (FIG. 3A and FIG. 3B).
[0146] To determine whether the prepared vaccine candidate virus stocks contained type I IFN, a commercial ELISA specific for human IFNβ (Invitrogen) was used. MA104 cells are epithelial Green Monkey kidney cells. According to the ELISA manufacturer, this Invitrogen ELISA is also suited for the detection of primate IFNβ other than human. For each vaccine candidate's virus stock, 100 μl served as assay input, while a virus stock from parental strain LoN94-13, cell culture medium, and medium from noninfected cells served as controls. For quantification of the obtained results, a calibration curve was included using a positive control of the ELISA manufacturer. All samples were measured in duplicates. Unlike the negative controls, virus stocks of the vaccine candidates contained considerable levels of type I IFN, while the virus stock of the parental virus showed IFN levels as low as the negative controls (FIG. 4).
[0147] To confirm the results obtained and to assess kinetics of type I IFN production in cells infected with the vaccine candidates, a time course experiment was performed using MA104 cells infected at a multiplicity of infection (MOI) of 0.001, respectively. Parental strain LoN94-13 served as negative control. While there were only very little and unaltered levels of type I IFN near background detectable for infection with parental strain LoN94-13, vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 induced considerable and increasing amounts of up to about 18 I.U. IFNβ per 25 μl sample volume from two days post infection on (FIG. 5).
[0148] It was experimentally assessed whether vaccine candidates containing genomic deletions in nsp1 demonstrate an increased sensitivity to type I IFN (FIG. 6). 5×105 MA104 cells were seeded into a well of a six-well plate and were either not infected (n.inf.) or infected with 800 infectious virus particles of one of the virus strains given on top, respectively. Cells then were either inoculated with 120 I.U. human IFNβ (+IFNβ, bottom row), respectively, or not (-IFNβ, top row). Three days post infection, immunofluorescence analysis specific for the PRRSV capsid protein was performed using monoclonal antibody SDOW17 (Rural Technologies). The total numbers of foci of PRRSV-infected cells per well are given below, respectively.
[0149] This experiment demonstrated that inoculation with type I IFN reduced the number of PRRSV infection events in cells after inoculation with a defined number of infectious virus particles, reflecting reduced viral infectivity of PRRSV when IFN was added. This reduction was 80-fold for wild type virus LoN94-13 (FIG. 6). In addition, for infection with wild type virus, foci of infected cells were smaller than in the well not inoculated with IFN (FIG. 6). However, for vaccine strains delta nsp1 XVII-1 and delta nsp1 XVIII-12, viral infectivity was reduced to zero when INF was added (FIG. 6). Thus, these vaccine candidates not only induce production of type I IFN in infected cells (FIG. 4 and FIG. 5), but also demonstrate increased sensitivity to type I INF when compared to wild type PRRSV (FIG. 6). This is reflected by their dramatically reduced viral infectivity when IFNβ is present.
[0150] Interestingly, the cells infected for the time course experiment summarized in FIG. 5 not only produced considerable amounts of IFNβ, but at the end of the experiment at six days post infection, cells infected with vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 showed signs of recovery from usually lytical PRRSV infection. While cells infected with parental strain LoN94-13 were fully lysed, cells infected with the vaccine candidates grew in a partially (delta nsp1 XVIII-12) or completely intact monolayer (delta nsp1 XVII-1). For the latter, only weak signs of a PRRSV-induced cytopathic effect were still detectable. Thus, the interferon production of infected cells together with the observed sensitivity of vaccine candidates to type I IFN correlated with partial or almost complete recovery of infected cells over time. It is reasonable to expect that type I IFN induction by the vaccine candidates together with their increased sensitivity to type I IFN will contribute to a significantly attenuated viral phenotype in the natural host. In particular, expected features of the vaccine candidates' attenuation in pigs include stimulation of the innate and specific immunity, both humoral and cellular, and less shedding and/or shortened viremia of the vaccine viruses.
[0151] To assess whether the PRRSV vaccine candidates are attenuated in the host, an animal experiment in piglets was performed.
[0152] Three groups, each of ten animals, were infected at study day 0 either with wild-type parental EU PRRSV strain Lon94-13 (WT group), or with delta nsp1 XVIII-12 (nsp1 group), or were not infected (Ch control group). Infection was applied by intramuscular injection to the neck at dosages of 106,56 TCID50 for LoN94-13 or 106,6 TCID50 for delta nsp1 XVIII-12, respectively. 21 days post vaccination, all animals were challenged with a virulent EU PRRSV strain being heterologous to LoN94-13 by intramuscular injection and intranasal inoculation at a total dosage of 3×106,52 TCID50. Animals were kept until the end of the experiment at day 31, ten days after challenge, and body temperatures were measured for all animals at days 0 (1 and 4 hours post vaccination), 1, 3, 5, 8, 10, 12, 14, 18, 20, 22, 24, 26, 28, and 31.
[0153] Mean body temperatures were determined for each animal for the time after vaccination but before challenge using measured body temperature data from all timepoints from day 0 through day 20. Subsequently, mean body temperatures were determined for each group (FIG. 7, (left-hand) columns). Error bars indicate standard deviations, respectively. Following the same procedure, mean body temperatures and standard deviations were determined for all groups for the time after challenge using measured body temperature data from all timepoints from day 22 through 31 (FIG. 7, (right-hand) columns).
[0154] The term "Significant(ly)" in the context of the following means either (i) p-values of 0.05 or lower as determined by the Dunnett test and obtained from comparing the nsp1 group with either the WT or the Ch control group for either of the two time periods investigated (before and after challenge) or (ii) p-values of 0.05 or lower when comparing the mean temperature change within a group and between the two time periods investigated (before and after challenge).
[0155] When comparing the determined mean body temperatures for the time after vaccination but before challenge (left hand columns) in between the three groups, animals from the WT group demonstrated a rise in body temperature of more than 0.4° C. when compared to animals from the noninfected Ch control group, thus demonstrating virulence of LoN94-13 in the infected host. In contrast, the nsp1 group showed a significant reduction in mean body temperature of more than 0.2° C. when compared to the WT group. Thus, since vaccination dosages were the same for the WT and the nsp1 group, the considerable reduction in increase of body temperature compared to WT demonstrates that the described mutation in the genome of delta nsp1 XVIII-12 has significantly reduced virulence of the WT parental strain LoN94-13 in the infected animal.
[0156] The significant rise in the mean body temperature of the Ch control group from before challenge to after challenge of 0.4° C. demonstrates virulence of the heterologous EU PRRSV challenge strain. The mean temperature of the WT group after challenge was slightly lower than before challenge, but not significantly reduced (FIG. 7). In contrast, the mean body temperature of the nsp1 group after challenge was significantly reduced by almost 0.2° C. when compared to that before challenge. Moreover, the body temperature of the nsp1 group after challenge was significantly reduced by almost 0.4° C. when compared to the Ch control group after challenge. Also, mean body temperature of the nsp1 group after challenge was significantly lower that that of the WT group after challenge by more than 0.2° C. Taken together, this demonstrates that a measurable and significant degree of protection from signs of disease induced by the applied challenge virus was conferred to pigs by vaccination with delta nsp1 XVIII-12. Since parental PRRSV strain LoN94-13 did not confer significant protection, it is evident that the described mutation in the genome of delta nsp1 XVIII-12 is causative for the observed significant protective technical effect.
[0157] Analogous experiments, wherein the vaccination was performed with lower amounts (105 TCID50) of delta nsp1 XVIII-12 showed results similar to the above described results (data not shown). Thus, in practice, a preferred amount of 103 to 105 TCID50 is sufficient for vaccination.
[0158] Taken together, the invention described here represents the first known viable PRRSV (EU) strains that contain mutations (deletions) in the nsp1 gene (nsp1β) that induce type I IFN (IFNβ) production in susceptible cells (MA104) and that show increased sensitivity to type I IFN (IFNβ). Moreover, the animal data demonstrates that (i) vaccine candidate delta nsp1 XVIII-12 is significantly attenuated in the host when compared to its parental PRRSV strain LoN94-13 and that (ii) vaccine candidate delta nsp1 XVIII-12 confers significant protection from signs of disease induced by challenge with a heterologous PRRSV strain while parental strain LoN94-13 does not. Thus, the described vaccine candidates or the described mutations therein, either alone or combined with other attenuating mutations, may serve as promising life attenuated PRRSV vaccines.
In the Sequence Listing
[0159] SEQ ID NO: 1 corresponds to LoN94-13 complete nsp1 protein,
[0160] SEQ ID NO: 2 corresponds to Lelystad virus complete nsp1 protein,
[0161] SEQ ID NO: 3 corresponds to VR2332 complete nsp1 protein,
[0162] SEQ ID NO: 4 corresponds to LoN94-13 complete nsp1 Alpha,
[0163] SEQ ID NO: 5 corresponds to Lelystad virus complete nsp1 Alpha,
[0164] SEQ ID NO: 6 corresponds to VR2332 complete nsp1 Alpha,
[0165] SEQ ID NO: 7 corresponds to LoN94-13 complete nsp1 Beta,
[0166] SEQ ID NO: 8 corresponds to Lelystad virus complete nsp1 Beta,
[0167] SEQ ID NO: 9 corresponds to VR2332 complete nsp1 Beta,
[0168] SEQ ID NO: 10 corresponds to LoN94-13 nsp1Beta NTD,
[0169] SEQ ID NO: 11 corresponds to Lelystad virus nsp1 Beta NTD,
[0170] SEQ ID NO: 12 corresponds to VR2332 nsp1 Beta NTD,
[0171] SEQ ID NO: 13 corresponds to LoN94-13 complete viral cDNA insert,
[0172] SEQ ID NO: 14 corresponds to Lelystad virus complete genome,
[0173] SEQ ID NO: 15 corresponds to VR2332 complete genome,
[0174] SEQ ID NO: 16 corresponds to delta nsp1 IX-10 complete nsp1 protein sequence,
[0175] SEQ ID NO: 17 corresponds to delta nsp1 XVII-1 complete nsp1 protein sequence,
[0176] SEQ ID NO: 18 corresponds to delta nsp1 XVIII-12 complete nsp1 protein sequence,
[0177] SEQ ID NO: 19 corresponds to delta nsp1 XIX-2 complete nsp1 protein sequence,
[0178] SEQ ID NO: 20 corresponds to delta nsp1 XX-9 complete nsp1 protein sequence,
[0179] SEQ ID NO: 21 corresponds to delta nsp1 IX-10 complete nsp1Beta protein sequence,
[0180] SEQ ID NO: 22 corresponds to delta nsp1 XVII-1 complete nsp1Beta protein sequence,
[0181] SEQ ID NO: 23 corresponds to delta nsp1 XVIII-12 complete nsp1 Beta protein sequence,
[0182] SEQ ID NO: 24 corresponds to delta nsp1 XIX-2 complete nsp1 Beta protein sequence,
[0183] SEQ ID NO: 25 corresponds to delta nsp1 XX-9 complete nsp1Beta protein sequence,
[0184] SEQ ID NO: 26 corresponds to delta nsp1 IX-10 nsp1 Beta NTD protein sequence,
[0185] SEQ ID NO: 27 corresponds to delta nsp1 XVII-1 nsp1Beta NTD protein sequence,
[0186] SEQ ID NO: 28 corresponds to delta nsp1 XVIII-12 nsp1Beta NTD protein sequence,
[0187] SEQ ID NO: 29 corresponds to delta nsp1 XIX-2 nsp1 Beta NTD protein sequence,
[0188] SEQ ID NO: 30 corresponds to delta nsp1 XX-9 nsp1Beta NTD protein sequence,
[0189] SEQ ID NO: 31 corresponds to delta nsp1 IX-10 complete viral cDNA insert sequence,
[0190] SEQ ID NO: 32 corresponds to delta nsp1 XVII-1 complete viral cDNA insert sequence,
[0191] SEQ ID NO: 33 corresponds to delta nsp1 XVIII-12 complete viral cDNA insert sequence,
[0192] SEQ ID NO: 34 corresponds to delta nsp1 XIX-2 complete viral cDNA insert sequence,
[0193] SEQ ID NO: 35 corresponds to delta nsp1 XX-9 complete viral cDNA insert sequence,
[0194] SEQ ID NO: 36 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 2 aa),
[0195] SEQ ID NO: 37 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 3 aa),
[0196] SEQ ID NO: 38 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 4 aa),
[0197] SEQ ID NO: 39 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 5 aa),
[0198] SEQ ID NO: 40 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 6 aa),
[0199] SEQ ID NO: 41 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 7 aa),
[0200] SEQ ID NO: 42 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 2 aa),
[0201] SEQ ID NO: 43 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 3 aa),
[0202] SEQ ID NO: 44 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 4 aa),
[0203] SEQ ID NO: 45 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 5 aa),
[0204] SEQ ID NO: 46 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 6 aa),
[0205] SEQ ID NO: 47 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 7 aa),
[0206] SEQ ID NO: 48 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 2 aa),
[0207] SEQ ID NO: 49 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 3 aa),
[0208] SEQ ID NO: 50 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 4 aa),
[0209] SEQ ID NO: 51 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 5 aa),
[0210] SEQ ID NO: 52 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 6 aa),
[0211] SEQ ID NO: 53 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 7 aa),
[0212] SEQ ID NO: 54 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 2 aa),
[0213] SEQ ID NO: 55 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 3 aa),
[0214] SEQ ID NO: 56 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 4 aa),
[0215] SEQ ID NO: 57 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 5 aa),
[0216] SEQ ID NO: 58 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 6 aa),
[0217] SEQ ID NO: 59 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 7 aa).
REFERENCE LIST
[0218] Allende, R., Laegreid, W. W., Kutish, G. F., Galeota, J. A., Wills, R. W., Osorio, F. A., 2000. Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J. Virol. 74, 10834-10837.
[0219] Ansari, I. H., Kwon, B., Osorio, F. A., Pattnaik, A. K., 2006. Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J. Virol. 80, 3994-4004.
[0220] Beura, L. K., Sarkar, S. N., Kwon, B., Subramaniam, S., Jones, C., Pattnaik, A. K., Osorio, F. A., 2010. Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. J. Virol. 84, 1574-1584.
[0221] Buddaert, W., Van, R. K., Pensaert, M., 1998. In vivo and in vitro interferon (IFN) studies with the porcine reproductive and respiratory syndrome virus (PRRSV). Adv. Exp. Med. Biol. 440, 461-467.
[0222] Chen, Z., Lawson, S., Sun, Z., Zhou, X., Guan, X., Christopher-Hennings, J., Nelson, E. A., Fang, Y., 2010. Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398, 87-97.
[0223] den Boon, J. A., Faaberg, K. S., Meulenberg, J. J., Wassenaar, A. L., Plagemann, P. G., Gorbalenya, A. E., Snijder, E. J., 1995. Processing and evolution of the N-terminal region of the arterivirus replicase ORF1a protein: identification of two papainlike cysteine proteases. J. Virol. 69, 4500-4505.
[0224] Haller, O., Weber, F., 2009. The interferon response circuit in antiviral host defense. Verh. K. Acad. Geneeskd. Belg. 71, 73-86.
[0225] Kim, O., Sun, Y., Lai, F. W., Song, C., Yoo, D., 2010. Modulation of type I interferon induction by porcine reproductive and respiratory syndrome virus and degradation of CREB-binding protein by non-structural protein 1 in MARC-145 and HeLa cells. Virology 402, 315-326.
[0226] Kroese, M. V., Zevenhoven-Dobbe, J. C., Bos-de Ruijter, J. N., Peeters, B. P., Meulenberg, J. J., Cornelissen, L. A., Snijder, E. J., 2008. The nsp1alpha and nsp1 papain-like autoproteinases are essential for porcine reproductive and respiratory syndrome virus RNA synthesis. J. Gen. Virol. 89, 494-499.
[0227] Li, H., Zheng, Z., Zhou, P., Zhang, B., Shi, Z., Hu, Q., Wang, H., 2010. The cysteine protease domain of porcine reproductive and respiratory syndrome virus non-structural protein 2 antagonizes interferon regulatory factor 3 activation. J. Gen. Virol. 91, 2947-2958.
[0228] Lopez, O. J., Osorio, F. A., 2004. Role of neutralizing antibodies in PRRSV protective immunity. Vet. Immunol. Immunopathol. 102, 155-163.
[0229] Meier, W. A., Galeota, J., Osorio, F. A., Husmann, R. J., Schnitzlein, W. M., Zuckermann, F. A., 2003. Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination. Virology 309, 18-31.
[0230] Miller, L. C., Laegreid, W. W., Bono, J. L., Chitko-McKown, C. G., Fox, J. M., 2004. Interferon type I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Arch. Virol. 149, 2453-2463.
[0231] Ostrowski, M., Galeota, J. A., Jar, A. M., Platt, K. B., Osorio, F. A., Lopez, O. J., 2002. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain J Virol. 76, 4241-4250.
[0232] Shi, X., Wang, L., Li, X., Zhang, G., Guo, J., Zhao, D., Chai, S., Deng, R., 2011a. Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-beta induction. Mol. Immunol. 48, 1568-1572.
[0233] Shi, X., Wang, L., Zhi, Y., Xing, G., Zhao, D., Deng, R., Zhang, G., 2010. Porcine reproductive and respiratory syndrome virus (PRRSV) could be sensed by professional beta interferon-producing system and had mechanisms to inhibit this action in MARC-145 cells. Virus Res. 153, 151-156.
[0234] Shi, X., Zhang, G., Wang, L., Li, X., Zhi, Y., Wang, F., Fan, J., Deng, R., 2011b. The Nonstructural Protein 1 Papain-Like Cysteine Protease Was Necessary for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 to Inhibit Interferon-beta Induction. DNA Cell Biol. 30, 355-362.
[0235] Snijder, E. J., Meulenberg, J. J., 1998. The molecular biology of arteriviruses. J. Gen. Virol. 79 (Pt 5), 961-979.
[0236] Song, C., Krell, P., Yoo, D., 2010. Nonstructural protein 1alpha subunit-based inhibition of NF-kappaB activation and suppression of interferon-beta production by porcine reproductive and respiratory syndrome virus. Virology 407, 268-280.
[0237] Sun, Y., Xue, F., Guo, Y., Ma, M., Hao, N., Zhang, X. C., Lou, Z., Li, X., Rao, Z., 2009. Crystal structure of porcine reproductive and respiratory syndrome virus leader protease Nsp1 alpha. J. Virol. 83, 10931-10940.
[0238] Xiao, Z., Batista, L., Dee, S., Halbur, P., Murtaugh, M. P., 2004. The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J. Virol. 78, 5923-5933.
[0239] Xue, F., Sun, Y., Yan, L., Zhao, C., Chen, J., Bartlam, M., Li, X., Lou, Z., Rao, Z., 2010. The crystal structure of porcine reproductive and respiratory syndrome virus nonstructural protein Nsp1beta reveals a novel metal-dependent nuclease. J. Virol. 84, 6461-6471.
Sequence CWU
1
1
841385PRTPorcine reproductive and respiratory syndrome virus 1Met Ser Gly
Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5
10 15 Phe Trp Asn Ala Gly Gln Val Tyr
Cys Thr Arg Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala
Ala Ile Gly 35 40 45
Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50
55 60 Ile Pro Gln Val
Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65 70
75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser
Gly Asn His Asn Phe Leu Gln 85 90
95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys
Leu Thr 100 105 110
Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp
115 120 125 Tyr Pro Ile Thr
Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130
135 140 Met His Val Ser Asp Gln Pro Phe
Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro
Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser
Ser Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu 195
200 205 Ser Asp Asp Ser Ala Asp Leu Glu
Ala Leu Pro Pro Glu Leu Glu Arg 210 215
220 Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His His
Pro Val Ser 225 230 235
240 Leu Ala Asp Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe
245 250 255 Ser Thr Tyr His
Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe 260
265 270 Asp Ser Lys Cys Trp Leu Ser Cys Phe
Leu Asp Gln Pro Ile Glu Val 275 280
285 Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln
Thr Lys 290 295 300
Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly 305
310 315 320 Ile Arg Ala Val Ile
Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu 325
330 335 Ser Cys Pro Gln Ser Trp Ile Arg His Leu
Thr Leu Asp Asp Asp Val 340 345
350 Thr Pro Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn
Thr 355 360 365 Glu
Pro Thr Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370
375 380 Gly 385 2385PRTPorcine
reproductive and respiratory syndrome virus 2Met Ser Gly Thr Phe Ser Arg
Cys Met Cys Thr Pro Ala Ala Arg Val 1 5
10 15 Phe Trp Asn Ala Gly Gln Val Phe Cys Thr Arg
Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Ser Pro Glu Leu Gln Asp Thr Asp Leu Gly Ala Val
Gly 35 40 45 Leu
Phe Tyr Lys Pro Arg Asp Lys Leu His Trp Lys Val Pro Ile Gly 50
55 60 Ile Pro Gln Val Glu Cys
Thr Pro Ser Gly Cys Cys Trp Leu Ser Ala 65 70
75 80 Val Phe Pro Leu Ala Arg Met Thr Ser Gly Asn
His Asn Phe Leu Gln 85 90
95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Ala
100 105 110 Pro Arg
His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Asn Trp 115
120 125 Tyr Pro Ile Thr Gly Pro Val
Pro Gly Met Gly Leu Phe Ala Asn Ser 130 135
140 Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr
His Val Leu Thr 145 150 155
160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe
165 170 175 Glu Glu Ala
His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180
185 190 Thr Asp Ser Ser Leu Asn Gly Arg
Ser Arg Met Met Trp Thr Pro Glu 195 200
205 Ser Asp Asp Ser Ala Ala Leu Glu Val Leu Pro Pro Glu
Leu Glu Arg 210 215 220
Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Asp 225
230 235 240 Leu Ala Asp Trp
Glu Leu Thr Glu Ser Pro Glu Asn Gly Phe Ser Phe 245
250 255 Asn Thr Ser His Ser Cys Gly His Leu
Val Gln Asn Pro Asp Val Phe 260 265
270 Asp Gly Lys Cys Trp Leu Ser Cys Phe Leu Gly Gln Ser Val
Glu Val 275 280 285
Arg Cys His Glu Glu His Leu Ala Asp Ala Phe Gly Tyr Gln Thr Lys 290
295 300 Trp Gly Val His Gly
Lys Tyr Leu Gln Arg Arg Leu Gln Val Arg Gly 305 310
315 320 Ile Arg Ala Val Val Asp Pro Asp Gly Pro
Ile His Val Glu Ala Leu 325 330
335 Ser Cys Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp
Val 340 345 350 Thr
Pro Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr 355
360 365 Glu Pro Thr Thr Ser Arg
Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370 375
380 Gly 385 3383PRTPorcine reproductive and
respiratory syndrome virus 3Met Ser Gly Ile Leu Asp Arg Cys Thr Cys Thr
Pro Asn Ala Arg Val 1 5 10
15 Phe Met Ala Glu Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu
Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly Val Leu Gly 35
40 45 Leu Phe Tyr Arg Pro Glu Glu
Pro Leu Arg Trp Thr Leu Pro Arg Ala 50 55
60 Phe Pro Thr Val Glu Cys Ser Pro Ala Gly Ala
Cys Trp Leu Ser Ala 65 70 75
80 Ile Phe Pro Ile Ala Arg Met Thr Ser Gly Asn Leu Asn Phe Gln Gln
85 90 95 Arg Met
Ile Arg Val Ala Ala Glu Leu Tyr Arg Ala Gly Gln Leu Thr 100
105 110 Pro Ala Val Leu Lys Ala Leu
Gln Val Tyr Glu Arg Gly Cys Arg Trp 115 120
125 Tyr Pro Ile Val Gly Pro Ala Pro Gly Val Ala Val
Tyr Ala Asn Ser 130 135 140
Leu His Val Ser Asp Lys Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Leu Pro
Leu Pro Gln Arg Pro Lys Pro Glu Asp Phe Cys Pro Phe 165
170 175 Glu Cys Ala Met Ala Thr Val Tyr
Asp Ile Gly His Asp Ala Val Met 180 185
190 Tyr Val Ala Glu Arg Lys Ile Ser Trp Ala Pro Arg Gly
Gly Asp Glu 195 200 205
Val Lys Phe Glu Ala Val Pro Gly Glu Leu Arg Leu Ile Ala Asn Arg 210
215 220 Leu Arg Thr Ser
Phe Pro Pro His His Thr Val Asp Met Ser Lys Phe 225 230
235 240 Ala Phe Thr Ala Pro Gly Cys Gly Val
Ser Met Arg Val Glu Arg Gln 245 250
255 His Gly Cys Leu Pro Ala Asp Thr Val Pro Glu Gly Asn Cys
Trp Trp 260 265 270
Ser Leu Phe Asp Ser Leu Pro Leu Glu Val Gln Asn Lys Glu Ile Arg
275 280 285 His Ala Asn Gln
Phe Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys 290
295 300 Tyr Leu Gln Arg Arg Leu Gln Val
Asn Gly Leu Arg Ala Val Thr Asp 305 310
315 320 Leu Asn Gly Pro Ile Val Val Gln Tyr Phe Ser Val
Lys Glu Ser Trp 325 330
335 Ile Arg His Leu Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe Glu
340 345 350 Asp Leu Leu
Arg Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Asp 355
360 365 Lys Glu Glu Lys Ile Phe Arg Phe
Gly Ser His Lys Trp Tyr Gly 370 375
380 4180PRTPorcine reproductive and respiratory syndrome
virus 4Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1
5 10 15 Phe Trp Asn
Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20
25 30 Ser Leu Leu Pro Pro Glu Leu Gln
Asp Ile Asp Leu Ala Ala Ile Gly 35 40
45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val
Pro Ile Gly 50 55 60
Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65
70 75 80 Ile Phe Pro Leu
Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85
90 95 Arg Leu Val Lys Val Ala Asp Val Leu
Tyr Arg Asp Gly Cys Leu Thr 100 105
110 Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys
Ser Trp 115 120 125
Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130
135 140 Met His Val Ser Asp
Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg
Gln Pro Phe Cys Pro Phe 165 170
175 Glu Glu Ala His 180 5180PRTPorcine reproductive
and respiratory syndrome virus 5Met Ser Gly Thr Phe Ser Arg Cys Met Cys
Thr Pro Ala Ala Arg Val 1 5 10
15 Phe Trp Asn Ala Gly Gln Val Phe Cys Thr Arg Cys Leu Ser Ala
Arg 20 25 30 Ser
Leu Leu Ser Pro Glu Leu Gln Asp Thr Asp Leu Gly Ala Val Gly 35
40 45 Leu Phe Tyr Lys Pro Arg
Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55
60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys
Cys Trp Leu Ser Ala 65 70 75
80 Val Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln
85 90 95 Arg Leu
Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Ala 100
105 110 Pro Arg His Leu Arg Glu Leu
Gln Val Tyr Glu Arg Gly Cys Asn Trp 115 120
125 Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu
Phe Ala Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro
Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165
170 175 Glu Glu Ala His 180
6180PRTPorcine reproductive and respiratory syndrome virus 6Met Ser Gly
Ile Leu Asp Arg Cys Thr Cys Thr Pro Asn Ala Arg Val 1 5
10 15 Phe Met Ala Glu Gly Gln Val Tyr
Cys Thr Arg Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly
Val Leu Gly 35 40 45
Leu Phe Tyr Arg Pro Glu Glu Pro Leu Arg Trp Thr Leu Pro Arg Ala 50
55 60 Phe Pro Thr Val
Glu Cys Ser Pro Ala Gly Ala Cys Trp Leu Ser Ala 65 70
75 80 Ile Phe Pro Ile Ala Arg Met Thr Ser
Gly Asn Leu Asn Phe Gln Gln 85 90
95 Arg Met Val Arg Val Ala Ala Glu Leu Tyr Arg Ala Gly Gln
Leu Thr 100 105 110
Pro Ala Val Leu Lys Ala Leu Gln Val Tyr Glu Arg Gly Cys Arg Trp
115 120 125 Tyr Pro Ile Val
Gly Pro Val Pro Gly Val Ala Val Phe Ala Asn Ser 130
135 140 Leu His Val Ser Asp Lys Pro Phe
Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Leu Pro Leu Pro Gln Arg Pro Lys Pro Glu Asp
Phe Cys Pro Phe 165 170
175 Glu Cys Ala Met 180 7 205PRTPorcine
reproductive and respiratory syndrome virus 7Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro
Glu Ser Asp Asp Ser 20 25
30 Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu
Ile 35 40 45 Leu
Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp 50
55 60 Glu Leu Ala Glu Ser Pro
Glu Asn Gly Phe Ser Phe Ser Thr Tyr His 65 70
75 80 Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val
Phe Asp Ser Lys Cys 85 90
95 Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu
100 105 110 Asp Tyr
Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser 115
120 125 Gly Lys Tyr Leu Gln Arg Arg
Leu Gln Val Asn Gly Ile Arg Ala Val 130 135
140 Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu
Ser Cys Pro Gln 145 150 155
160 Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe
165 170 175 Val Arg Leu
Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr 180
185 190 Leu Arg Ile Phe Arg Phe Gly Ala
His Lys Trp Tyr Gly 195 200 205 8
205PRTPorcine reproductive and respiratory syndrome virus 8Ser Ser Val
Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Leu Asn Gly Arg Ser Arg Met Met
Trp Thr Pro Glu Ser Asp Asp Ser 20 25
30 Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln
Val Glu Ile 35 40 45
Leu Ile Arg Ser Phe Pro Ala His His Pro Val Asp Leu Ala Asp Trp 50
55 60 Glu Leu Thr Glu
Ser Pro Glu Asn Gly Phe Ser Phe Asn Thr Ser His 65 70
75 80 Ser Cys Gly His Leu Val Gln Asn Pro
Asp Val Phe Asp Gly Lys Cys 85 90
95 Trp Leu Ser Cys Phe Leu Gly Gln Ser Val Glu Val Arg Cys
His Glu 100 105 110
Glu His Leu Ala Asp Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val His
115 120 125 Gly Lys Tyr Leu
Gln Arg Arg Leu Gln Val Arg Gly Ile Arg Ala Val 130
135 140 Val Asp Pro Asp Gly Pro Ile His
Val Glu Ala Leu Ser Cys Pro Gln 145 150
155 160 Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val
Thr Pro Gly Phe 165 170
175 Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr
180 185 190 Ser Arg Ile
Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 200
205 9 203PRTPorcine reproductive and respiratory
syndrome virus 9Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met Tyr Val
Ala Glu 1 5 10 15
Arg Lys Ile Ser Trp Ala Pro Arg Gly Gly Asp Glu Val Lys Phe Glu
20 25 30 Ala Val Pro Gly Glu
Leu Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35
40 45 Phe Pro Pro His His Thr Val Asp Met
Ser Lys Phe Ala Phe Thr Ala 50 55
60 Pro Gly Cys Gly Val Ser Met Arg Val Glu Arg Gln His
Gly Cys Leu 65 70 75
80 Pro Ala Asp Thr Val Pro Glu Gly Asn Cys Trp Trp Ser Leu Phe Asp
85 90 95 Ser Leu Pro Leu
Glu Val Gln Asn Lys Glu Ile Arg His Ala Asn Gln 100
105 110 Phe Gly Tyr Gln Thr Lys His Gly Val
Ser Gly Lys Tyr Leu Gln Arg 115 120
125 Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Leu Asn
Gly Pro 130 135 140
Ile Val Val Gln Tyr Phe Ser Val Lys Glu Ser Trp Ile Arg His Leu 145
150 155 160 Lys Leu Ala Gly Glu
Pro Ser Tyr Ser Gly Phe Glu Asp Leu Leu Arg 165
170 175 Ile Arg Val Glu Pro Asn Thr Ser Pro Leu
Ala Asp Lys Glu Glu Lys 180 185
190 Ile Phe Arg Phe Gly Ser His Lys Trp Tyr Gly 195
200 1052PRTPorcine reproductive and respiratory
syndrome virus 10Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp
Ser Ser 1 5 10 15
Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser
20 25 30 Ala Asp Leu Glu Ala
Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35
40 45 Leu Ile Arg Ser 50
1152PRTPorcine reproductive and respiratory syndrome virus 11Ser Ser Val
Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Leu Asn Gly Arg Ser Arg Met Met
Trp Thr Pro Glu Ser Asp Asp Ser 20 25
30 Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln
Val Glu Ile 35 40 45
Leu Ile Arg Ser 50 1248PRTPorcine reproductive and
respiratory syndrome virus 12Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val
Met Tyr Val Ala Glu 1 5 10
15 Arg Lys Ile Ser Trp Ala Pro Arg Gly Gly Asp Glu Val Lys Phe Glu
20 25 30 Ala Val
Pro Gly Glu Leu Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35
40 45 13 15116DNAPorcine
reproductive and respiratory syndrome virus 13atgatgtgta gggtattccc
cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc
caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg
cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta
cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct
gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct
cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct
aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc
gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac
ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct
cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg
ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct
ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc
tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg
gactcctccc ccaacggtcg gcctcgcatg atgtggacgc 840cggaatccga tgattcagcc
gacctagagg cgctaccgcc tgagctagaa cgtcaggtcg 900aaatcctcat tcggagtttt
cctgcccatc accctgtcag cctggccgac tgggagctcg 960ctgagtcccc tgagaacggt
ttttccttca gcacgtacca ttctggtggt tatcttgtcc 1020aaaaccccga cgtgtttgac
agcaagtgct ggctctcctg tttcttggat cagccgatcg 1080aagtgcgcct ctatgaggat
tatctggcta acgctttcgg ttaccaaacc aagtggggcg 1140tgtctggtaa gtacctccag
cgcaggcttc aagtcaacgg tattcgtgct gtaatcgatc 1200ctgatggccc cattcacgtt
gaagcgctgt cttgccccca atcttggatc aggcacctga 1260ctctggacga tgacgtcacc
ccaggattcg ttcgcctaac atccctccgc attgtgccga 1320acacagagcc cactactctc
cgaatctttc ggtttggagc gcataagtgg tatggcgctg 1380ccggcaaacg ggctcgtgcc
aagcgtgccg ctaaaagtga gaagggtccg gctcccactc 1440ccaaggttgc cccgccagcc
cccacctgcg gaattgttac ctactctcca ccaacagacg 1500ggtcttgcgg ttggcacgtc
cttgccgcca taatgaaccg aatgatgaac ggtgacttca 1560tgtcccctct ggcccagtac
aacagaccag aggatgattg ggcttctgat tacgatcttg 1620cccaggcgat tcaatgtctg
cggttgcctg ctaccatagt tcggaatcgt gcctgtccca 1680acgccaagta cctcataaaa
ctcaacggag tccactggga ggtagaggtg aggtcaggga 1740tggcccctcg ctcccttccc
cgcgagtgcg tagtcggcgt ttgttccgaa ggctgtgccg 1800catcgcctta cccagaaaac
gggctaccta aacgagcgtt tgaggccttg gcgtctgctt 1860acagactacc ttccgattgt
gtcagttctg gtattgctga ctttcttgct aacccccctc 1920aggaattctg gactcttgac
aaaatgttga cctccccgtc accggagcgg tctggttttt 1980ccagtctgta caaattgcta
ttagaggttg tcccgcagaa atgcggagcc acggaagggg 2040ctttcaccta tgctgttgag
agaatgctga aggattgccc gagctccgaa caggccatgg 2100cccttctggc aaaaattaaa
gttccatcct caaaggcccc gtctgtgtcc ctggacgagt 2160gtttccctac ggatgtttca
gccgatttcg aaccagcatc tcaggagagg tcccaaaatt 2220ccagcgctgc tgttgtcctg
cattcaccga atgcaaaaga gttcgaggaa gcagctccag 2280gggaagttca ggagggtggc
cacaaggccg tccactctgc actccctgcc gggggtccta 2340acaataagca ggcacagctg
gttgccggtg agcaactgaa gctcggcggt tgtggttcgg 2400tagttgggaa tgcacatgaa
ggtgttctgg tcccacctgg tccaattaat ttgacaagcg 2460gggatttacc ctcctcaggc
tccatgaaag aagatatgct caatagccgg gaggacgaac 2520cactggattt gtcccaacca
gcaacagctg tcacaacgac tcttatggga gagctaacac 2580ccgactacct aggttctgat
actggtgccc tccccgtcac cgtccgaaaa tttgtcccga 2640cggggcctat actccgtcat
gttgagcact gcagcacggg gtcgggcgat agcagttcgc 2700ctttggatct gtctggtgcg
caaaccccgg accagccttt aaatctgtcc ctggcggctt 2760ggccagtgag gaccaccgcg
tctgatcctg gctgggtcca cggtagacgc gagcctgtct 2820ttgtaaagcc tcgagatgtt
ttctctgatg gcgattcagc ccttcagttc ggggagcttt 2880ctgaatccag ctctgtcatc
gagtttgatc gggcaaaaga tgctcaggtg gctgacgccc 2940ctgtcggtct gacgacttcg
gacgaggccc tctccgcagt cgatcctttc gagttttccg 3000aactcaagcg cccacgtttc
tccgcacaag ccttaattga ccgaggcggc ccacttgccg 3060atgtccatgc aaaaataaag
aaccgggtat atgaacagtg cctccaagct tgtgagcccg 3120gtagtcgcgc aaccccagct
accagggagt ggctcgacaa aatgtgggag agagtggaca 3180tgaaaacctg gcgctgcacc
tcacagttcc aagctggtca cattcttgcg tccctcaaat 3240tcctccctga catgattcaa
gacacaccgc ctcctgttcc caggaagaac cgagccagtg 3300ataaagctgg cctgaaacaa
ctagtggcac agtgggatag gagattgagt tcaacccccc 3360ccccaaaacc ggttgggccg
gtacttgacc gggtcgatcc tccgcctacg ggtacccggc 3420aagaagacgt caccccctcc
gatgggccac cccatgcgcc ggatggtcga gtgagtacgg 3480gtgggagttg gaaaggcctt
atgctttccg gcacccgtct cacggggtcc atcagtcatc 3540gcctcatgac atgggttttt
gaagttgtct cccatctccc agcttttatg ctcacacttt 3600tctcgccgcg gggctctatg
gctccaggcg attgggtttt tgcaggtgtt gttttacttg 3660ctctcctgct ctgtcgttct
tacccaatat tcgggtgcct tcccttattg ggtgtctttt 3720ctggtactgt gcggcgtgtt
cgtctgggtg tttttggctc ttggatggct tttgctgtat 3780ttttattctc gactccatcc
aacccagtcg gttcttcttg taaccacgat tcgccggagt 3840gtcatgctga gcttctggct
cttgagcagc gccaactttg ggaacctgtg cgcggccttg 3900tggtcggccc atcgggcctc
ttatgtgtca ttcttggcaa gttactcggt gggtcacgtt 3960atctctggca tgttctccta
cgtctatgcc tgcttgcaga tttggccctt tctcttgttt 4020atgtggtgtc ccaggggcgt
tgtcacaagt gttggggaaa gtgtataagg acagctcctg 4080ctgaggtggc ttttaatgta
tttcctttct cgcgcgccac ccgtagttct cttgtatcct 4140tatgtgatcg attccaaacg
ccaaaaggag ttgatcccgt gcacttggcg acaggttggc 4200gcgggtgctg gcgtggtgag
agtcctatcc atcaaccaca ccaaaagccc atagcttacg 4260ccaatttgga tgaaaagaag
atatctgccc aaacggttgt tgctgtccca tacgatccca 4320atcaggctat caaatgtctg
aaagttctgc aggcgggagg ggctatcgtg gatcagccta 4380cgcctgaggt cgttcgtgtg
tccgagatcc ccttctcagc cccatttttc ccaaaagttc 4440cagtcaaccc agattgtagg
gtcgtggtgg attcggacac ttttgtggct gcggttcgtt 4500gtggttattc gacatcacaa
ctggtcctgg gccagggcaa ctttgccaag ttaaatcaaa 4560ccccccccag gaactctatc
tccaccaaag cgactggtgg ggcctcttat acttttgctg 4620tggctcaagt gtctgtgtgg
acccttgttc atttcgtcct cggtctttgg ctcacgtcac 4680ctcaagtgtg tggtcgagga
accgctgacc catggtgttc aagtccattt tcatatccta 4740cctatggccc cggggttgtg
tgctcctctc gactttgtgt gtctgctgac ggggtcaccc 4800ttccattgtt ctcagccgtg
gcacaactct ctggtaggga ggtggggatt tttattttag 4860tgctcgtctc ctttattgcc
ttggcccacc gcatggctct taaggcagac atgttagtag 4920tctttttggc tctttgtgct
tatgcctggc ccatgagctc ctggttgatc tgcttctttc 4980ctatactctt gaggtgggtt
acccttcacc ctctcactat gctttgggtg cattcattct 5040tgatgttttg tctcccagca
gccggcgtcc tctcactggg gataactggc ctcctctggg 5100caatcggccg ctttactcag
gttgccggaa tcattacacc ttatgatatc caccagtaca 5160cctctgggcc acgtggtgca
gccgctgtgg ccacagcccc agaaggcact tacatggccg 5220ccgtccggag agctgcttta
accgggcgga ctttaatctt caccccgtcc gcagttggat 5280cccttctcga aggtgctttc
aggactcata aaccctgcct taacaccgtg aatgtcgtag 5340gctcctccct tggttccgga
ggggttttca ccattgacgg aaaaaaaatt gtcgtcactg 5400ctgcccatgt gctgaacggc
gacacagcta gagtcaccgg tgattcctac aaccgcatgc 5460acactttcaa gactaatggt
gactatgcct ggtcccatgc tgataactgg cagggcgctg 5520cccctgtggt caaggttgcg
aaagggtatc gcggtcgtgc ctactggcaa acatcaactg 5580gtgtcgagcc tggtgttatt
gggaatgggt tcgccttctg tttcaccaac tgcggcgatt 5640cggggtcacc cgttatctca
gaatctggtg atcttatcgg aatccacacc ggttcaaaca 5700aacttggttc tggtcttgtg
acaacccctg aaggggagac ctgtaccatc agagaaacca 5760agctttctga cctttccaga
catttcgcag gcccaagcgt tcctcttggg gacatcaaat 5820tgagtccggc catcatccct
gatgtgacat ccattccgag tgacttggca tcgctcctag 5880cttccgtccc tgtaatggaa
ggcggcctct cgaccgttca acttttgtgt gtctttttcc 5940tcctctggcg catgatgggc
catgcctgga cgcccattgt tgccgtgggc ttctttttgc 6000tgaatgaaat tcttccagca
gttttggttc gagccgtgtt ttcttttgca ctctttgtgc 6060ttgcatgggc caccccctgg
tctgcacaag tgttgatgat tagacttctc acggcatctc 6120tcaaccgcaa caaactttct
cttgcgttct acgcactcgg aggtgttgtt ggtttggctg 6180ctgaaatcgg gacttttgct
ggtaaattgt ctgaattgtc tcaagctctt tcgacatact 6240gtttcttacc tagggtcctt
gctatgacca gctgtgttcc catcatcatc attggtggac 6300tccatgccct cggtgtgatt
ctgtggttat tcaaataccg gtgcctccac aacatgctgg 6360ttggtgatgg aagcttttca
agcgctttct tcctacggta ttttgcagag ggcaatctca 6420ggagaggtgt ttcacagtcc
tgtggcatga gtaacgagtc cctgacggct gctttggctt 6480gcaagttgtc acaggctgac
cttgattttt tgtccagctt aacgaacttc aagtgctttg 6540tatctgcttc aaacatgaaa
aatgctgccg gccagtacat tgaagcagct tatgccaggg 6600ccctgcgtca agagttggcc
tctttagtcc agattgacaa aatgaaagga gttttgtcca 6660agctagaggc ctttgctgaa
acggccactc cgtccctcga cgtaggtgac gtgattgttc 6720tacttggaca acatcctcac
ggatccgttc tcgatattaa tgtggggact gaaaggaaaa 6780ctgtatccgt gcaagagacc
cggagcctag gcggctccaa gttcagtgtt tgtactgttg 6840tgtcaaacac acccgtggac
gccttagccg gtattccact ccagacacca accccccttt 6900tcgagaatgg cccgcgtcat
cgcagcgagg aggacgatct taaagtcgag aggatgaaga 6960aacactgcgt gtccctcggc
ttccacaaca ttaacggtaa agtttactgc aagatttggg 7020acaagtctac cggtgacgcc
ttttacactg atgattcccg gtacacccaa gactatgctt 7080ttcaggacag gtcagctgac
tatagagaca gggactacga gggtgtgcaa accgcccccc 7140aacagggatt tgatccaaag
tctgaaaccc ctgttggtac cgttgtgatc ggcggtatta 7200cgtacaacag gtatttggtc
aaaggtaagg aggttctggt tcccaagcct gacaactgcc 7260ttgaagctgc caagctgtcc
cttgagcaag ctctcgctgg gatgggccaa acttgtgacc 7320ttacagctgc cgaggtggaa
aagctaaagc gcatcattgg tcaacttcaa ggattgacca 7380ctgagcaggc tttaaactgt
tagccgccag cggcttgacc cgctgtggcc gcggcggcct 7440agttgtaact gaaacggcgg
taaaaattgt caaataccac agcagaactt ttaccttagg 7500ctctttagac ctaaaagtca
cttccgaggt ggaggtgaag aagtcaaccg agcagggcca 7560cgctgttgtg gcaaacttgt
gttctggtgt cgtcttgatg agacctcacc caccgtccct 7620tgtcgacgtt cttctgaaac
ccggacttga cataacaccc ggcattcaac cagggcatgg 7680ggccgggaat atgggcgtgg
acggttccat ttgggatttt gaaaccgcac ccacaaaggc 7740tgaactcgag ttatccaagc
aaataattca agcatgtgaa gtcaggcgcg gggatgcccc 7800gaacctccag ctcccttaca
agctctatcc tgttagaggg gatcctgagc ggcataaagg 7860ccacctcatc aataccaggt
ttggagactt accttacaaa actcctcaag acaccaagtc 7920cgcaatccac gcggcttgtt
gcctgcaccc caacggggcc cccgtgtctg atggtaaatc 7980cacactaggt accactcttc
aacatggctt cgagctttat gtccctactg tgccctatag 8040tgtcatggag taccttgatt
cacgcccaga cacccctttt atgtgcacta aacacggcac 8100ttccaaggct gctgcagagg
acctccaaaa atacgaccta tccacccaag gatttgtcct 8160gcctggggtc ctacgcctag
tgcgcaggtt catctttggc cacattggca aggcaccgcc 8220attgttcctc ccatcaactt
atcccgccaa gaactccatg gcaggtatta atggtcagag 8280gttcccaaca aaggatgttc
aaagtatacc tgaaattgat gaaatgtgtg cccgcgccgt 8340caaggagaat tggcaaactg
tgacaccttg caccctcaag aaacagtatt gttctaggcc 8400caaaaccagg accatcctgg
gcactaacaa cttcatagcc ttggctcata gatcggcgct 8460cagtggtgtt acccaggcat
tcatgaagaa ggcttggaag tccccaatag ccttagggaa 8520aaacaaattc aaggagctgc
attgcactgt cgctggcagg tgcctcgagg ccgacttggc 8580ttcctgtgac cgcagcaccc
ctgccattgt gaggtggttc actacccacc tcctatatga 8640acttgcagga tgtgaagaat
atctacctag ctatgtgctt aactgttgcc atgaccttgt 8700ggcgacgcag gatggtgctt
tcacaaaacg cggtggcctg tcgtctggag acccagtcac 8760cagtgtgtcc aacactgtgt
actcactggt gatttatgcc cagcacatgg tactatctgc 8820cctgaaaatg ggtcatgaaa
ttggcctcaa gttcctcgaa gaacaactca aatttgagga 8880ccttcttgaa atccagccta
tgttagtata ctctgatgat cttgtcttgt acgcagaaaa 8940gcccaccttc cccaactatc
attggtgggt cgagcatctt gacctgatgt tgggctttaa 9000aacggaccca aagaaaaccg
tcataactga taaacccagt ttcctcgggt gcagaatcga 9060agcagggcga cagctagtcc
ccaatcgcga ccgcatcctg gctgctcttg catatcacat 9120gaaggcgcag aacgcctcag
agtattatgc gtctgctgcc gcaatcctga tggattcatg 9180tgcttgcatt gaccacgatc
ctgaatggta tgaggacctc atctgtggca ttgcccgatg 9240cgctcgcctg gacggttata
gctttccagg tccggcattt ttcatgtcca tgtgggagaa 9300gctgaggagt cataatgaag
ggaagaaatt ccgccactgc ggcatctgcg acgccaaagc 9360cgactacgcg gctgcttgtg
ggcttgattt gtgtttgttt cattcgcact ttcaccaaca 9420ctgccctgtc actctgagct
gcggtcacca tgccggttcg aaggaatgtt cgcagtgtca 9480gtcacctgtt ggggccggca
gatcccctct tgatgctgtg ctggaacaaa ttccatacaa 9540acctcctcgc actgtcatca
tgaaggtggg taataaaaca acggcccttg atccggggag 9600gtaccagtcc cgtcgaggtc
tcgttgcagt caagaggggt attgcgggta atgaagttga 9660tcttgctgat ggagattacc
aagtggtacc tcttctgccg acttgcaaag atataaacat 9720ggtgaaggtg gcttgcaacg
tactactcag caagttcata gtagggccac caggttccgg 9780gaagaccacc tggctactaa
gtcaagttca ggacgatgat gtcatttaca cacccactca 9840tcagaccatg tttgacatag
ttagtgctct caaagtttgc aggtattcca ttccaggagc 9900ctcgggactc cctttcccac
cgcctgccag gtccgggccg tgggttaggc tcattgccag 9960cgggcacgtc cctggccgag
tatcatacct cgatgaggcc ggatattgca atcatctgga 10020cattcttaga ctgctttcca
aaacacccct tgtgtgtttg ggtgaccttc agcaacttca 10080cccagtcggc tttgattcct
attgttatgt gttcgatcag atgcctcaga agcagttgac 10140caccatttac agatttggcc
ccaacatctg cgcagccatc cagccttgtt acagggagaa 10200acttgaatct aaggctagga
acaccagggt ggtttttacc acccggcctg tggcctttgg 10260ccaggtgctg acaccatatc
acaaagatcg cgtcggctcc gcgattacca tagactcatc 10320ccagggggcc acctttgaca
ttgtaacatt gcatctacca tcgccaaagt ccctaaataa 10380gtcccgggca cttgtggcca
tcacacgggc aagacacggg ttgttcattt atgaccctca 10440caaccagctc cgggagtttt
tcaacctaac ccctgagcgc actgattgta accttgtgtt 10500cagccgtgga gatgagctgg
tggtcctgaa tgcagataat gcagtcacaa ccgtggcgaa 10560ggccttagag acaggtccaa
ctcaatttcg agtgtcagac ccgaggtgca agtctctctt 10620agccgcttgc tcggccagtc
tggaagggag ctgcatgccg ctaccgcaag tggcgcataa 10680cctggggttt tacttctccc
cagacagtcc agtatttgca cctctgccaa aagagttggc 10740gccacattgg ccagtggtta
cccatcagaa taatcgggcg tggcctgatc gacttgtcgc 10800tagtatgcgt ccaattgacg
cccgctacag caagccgatg gtcggtgcag ggtatgtggt 10860cggaccgtcc accttccttg
gtactcctgg agtggtgtca tactatctca cactatacat 10920caggggtgag ccccaggcct
tgccagaaac acttgtttca acaggacgta tagccacaga 10980ttgtcgggag tatctcgacg
cggctgagga agaggcagca aaagaacttc cccacgcgtt 11040cattggcgat gtcaaaggca
ccacagttgg ggggtgtcat cacattacat caaaatacct 11100acctaggtcc ctgcctaaag
actctgttgc cgtagttgga gtaagttcgc ctggcagggc 11160tgctaaagcc gtatgcaccc
tcaccgatgt gtacctccct gaactccggc catatctgca 11220acctgagacg gcatcaaaat
gctggaaact caaattagac ttcagggacg tccgactaat 11280ggtctggaaa ggagccaccg
cctactttca attggaaggg ctcacatggt cggcgctgcc 11340tgactatgcc aggtttattc
agctgcccaa aaacgctgtt gtatacatcg atccgtgcat 11400aggaccggca acagccaatc
gtaaagtcgt acgaaccaca gattggcggg ccgacctggc 11460agtgacgccg tatgattacg
gtgcccggaa cattttgaca acagcctggt tcgaggacct 11520cgggccgcag tggaagattc
tggggttgca gccctttagg cgggcgtttg gctttgaaaa 11580cactgaggat tgggcaatcc
ttgcatgctg catgagtgac ggcaaggact acactgacta 11640taactggaat tgcgttcgac
aacgcccaca cgctatccat ggacgcgctc gtgaccatac 11700gtaccacttt gcccctggca
ctgaattgca agtggagctc ggtaaacccc ggctgccacc 11760tgagcaagta ccgtgaattc
ggagtgatgc aatggggtca ctgtggagta aaatcagcca 11820gctgttcgtg gacgccttca
ctgaattcct tgttagtgtg gttgatattg tcatcttcct 11880tgccatattg tttgggttca
ccgtcgcagg atggttactg gtctttcttc tcagagtggt 11940ttgctccgcg cttctccgtt
cgcgctctgc cattcactct tccgaactat cgaaggtcct 12000atgagggctt actacctaat
tgcagaccgg atgttccaca atttgcattt aagcaccctt 12060tgggtatgtt ttggcacatg
cgggtttccc acctaattga tcagatggtc tctcgccgca 12120tctaccagac catggaacat
tcaggtcaag cggcctggaa gcacgtggtc agtgaggcta 12180ctcttacaaa attgtcagaa
ctcgacatag ttctccactt ccaacacctg gccgcagtgg 12240aggcggactc ttgtcgcttc
ctcagctcac gacttgtgat gctgaaaaat cttgccgttg 12300gcaatgtgag cttgcagtac
aacaccacgt tgaaccgcgt tgagctcatc ctccccacac 12360caggtacgag gcccaaattg
accgatttca gacaatggct catcagtgtg cacgcttcca 12420ttttttcctc tgtagcctca
tcagttactt tgttcatagt gctttggctt cgaattccag 12480ccgtacgcta tgtttttggt
ttccattggc ccatggcaac acgtcattcg agctgaccat 12540taattacact atatgcatgc
cctgtcttac cagccaagcg gctcaacaaa ggctcgaacc 12600cggtcgtaac atgtggtgca
aaataggaca caccacgtgc gaggagcgtg accatgatga 12660gttgtcaatg tccatcccgt
ccgggtacga caacctcaaa cttgaaggtt attacgcttg 12720gctggctttt ttgtcctttt
cctacgcggc ccaattccat ccggagttgt ttggaatagg 12780gaatgtgtcg cgcgtctttg
tggataaacg acaccagttc atttgtgccg agcatgacgg 12840agataattca accgtatcta
ccggacacaa catctccgca tcatatgcgg catattatca 12900ccaccaaata gacgggggca
attggttcca tttggaatgg ctgcggccgc tcttttcctc 12960ttggctagtg ctcaacatat
catggtttct gaggcgttcg cctgcaagcc ctgtttctcg 13020acgcatctat cagatattaa
gaccaatacg accgcggctg ccggtttcat ggtccttcaa 13080gacatcagtt gcctccgacc
tcacagggtc tcagcatcgc aagagaacat tcccttcgga 13140aagtcgtcac aatgtcgtga
agccgtcggt actccccagt acattacgat gactgctaat 13200gtgaccgacg aatcatattt
gtacaacgcg gacttgctaa tgctttccgc gtgccttttc 13260cacgcctcag aaatgagcga
gaaaggcttc aaagttatct ttggaaacgt ctccggcgtt 13320gtttcagctt gtgtcaattt
cacagattat gtggcccatg taacccaaca tacccaacag 13380catcatctgg taattgatca
cattcggtta ctgcatttcc tgacaccatc tgcaatgagg 13440tgggctacaa ccattgcttg
tttgttcgcc attctcttag cgatatgaga tgttctcaca 13500aattggggcg tttcttgact
ccgcactctt gcttctggtg gctttttttg ctgtgtaccg 13560gcttgtcctg gtcctttgcc
gatggcaacg gcaacagctc gacacgccaa tacatatata 13620acttgacgat atgcgagctg
aatgggaccg tctggttgtc cagtcatttt gattgggcag 13680tcgagacctt tgtgctttac
ccggtggcca ctcatatcct ctcactgggt tttctcacaa 13740caagccattt ttttgatgcg
ctcggtctcg gcgctgtgtc cactacggga tttcttggcg 13800ggcggtatgt acttagcagc
gtgtacggcg cctgcgcctt cgcagcgctt gtatgttttg 13860tcatccgtgc tgctaaaaat
tgcatggctt gccgttatgc ccgcacccgg ttcaccaact 13920tcatcgtgga cgaccggggg
aagatccatc gatggaagtc cccaatagtg gtagagaaat 13980taggcaaagc tgacatcggc
ggcgaccttg tcaccatcaa acatgttgtc ctcgaaggag 14040tcaaagctca acctttgacg
aggacatcgg cggagcaatg ggaagcctag atgatttttg 14100caatgatcct accgccgcac
agaagcttgt gctggcattt agtatcacat acacacctat 14160aatgatatac gccctcaagg
tgtcacgcgg ccggctccta ggactgttac acatcctgat 14220atttctgaac tgttctttca
cgttcggata catgacatac gtgcactttc aatccactaa 14280ccgtgtcgcg cttactatgg
gggcggtcgt tgcccttttg tggggcattt acagctttat 14340agaatcatgg aagtttgtca
cttccagatg caggttgtgt tgcctaggcc ggcgatacat 14400tctggcccct gcccaccacg
tagaaagtgc tgcaggcctc cattcaatcc cagcgtctgg 14460taaccgagca tacgctgtga
gaaagcccgg actaacatca gtgaacggca ctctagtacc 14520aggacttcgg agcctcgtgt
tgggcggcaa acgagctgtt aaacgaggag tggttaacct 14580cgtcaagtat ggccggtaaa
aaccagagcc agaagaaaaa gaaaaacaca gctcctatgg 14640ggagtggcca gccagtcaat
caactgtgcc aattgctggg cacaatgata aagtcccagc 14700gccagcggcc taggggagga
caggccaaaa tgaaaaagcc tgagaagcca catttccccc 14760tagctgctga agatgacatc
cggcaccatt tcacccagac cgagcgttcc ctttgcttgc 14820aatcgatcca gacggccttc
aatcaaggcg caggaactgc gtcgctttca tccagcggga 14880aggtcagttt tcaggttgag
ttcatgctgc cggtcgctca tacggtgcgc ctgattcgcg 14940taacttccac atccgccagt
cagggtgcaa gttaatttga tagttaggtg aatggccgcg 15000attggcgtgt ggcctctgag
tcacctattc aattagggcg atcacatggg ggttagactt 15060aattggcgag aaccatgtga
ccgaaattaa aaaaaaaaaa aaaaaaaaaa aaaaaa 151161415111DNAPorcine
reproductive and respiratory syndrome virus 14atgatgtgta gggtattccc
cctacataca cgacacttct agtgtttgtg taccttggag 60gcgtgggtac agccccgccc
caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgcgccg
cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180tccggagagc acctgcttta
cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct
gcccgggtat tttggaacgc cggccaagtc ttttgcacac 300ggtgtctcag tgcgcggtct
cttctctctc cagagcttca ggacactgac ctcggtgcag 360ttggcttgtt ttacaagcct
agggacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggaatg tactccatcc
gggtgctgtt ggctctcagc tgttttccct ttggcgcgta 480tgacctccgg caatcacaac
ttcctccaac gacttgtgaa ggttgctgat gttttgtacc 540gtgacggttg cttggcacct
cgacaccttc gtgaactcca agtttacgag cgcggctgca 600actggtaccc gatcacgggg
cccgtgcccg ggatgggttt gtttgcgaac tccatgcacg 660tatccgacca gccgttccct
ggtgccaccc atgtgttgac taactcgcct ttgcctcaac 720aggcttgtcg gcagccgttc
tgtccatttg aggaggctca ttctagcgtg tacaggtgga 780agaaatttgt ggttttcacg
gactcctccc tcaacggtcg atctcgcatg atgtggacgc 840cggaatccga tgattcagcc
gccctggagg tactaccgcc tgagttagaa cgtcaggtcg 900aaatcctcat tcggagtttt
cctgctcatc accctgtcga cctggccgac tgggagctca 960ctgagtcccc tgagaacggt
ttttccttca acacgtctca ttcttgcggt caccttgtcc 1020agaaccccga cgtgtttgat
ggcaagtgct ggctctcctg ctttttgggc cagtcggtcg 1080aagtgcgctg ccatgaggaa
catctagctg acgccttcgg ttaccaaacc aagtggggcg 1140tgcatggtaa gtacctccag
cgcaggcttc aagttcgcgg cattcgtgct gtagtcgatc 1200ctgatggtcc cattcacgtt
gaagcgctgt cttgccccca gtcttggatc aggcacctga 1260ctctggatga tgatgtcacc
ccaggattcg ttcgcctgac atcccttcgc attgtgccga 1320acacagagcc taccacttcc
cggatctttc ggtttggagc gcataagtgg tatggcgctg 1380ccggcaaacg ggctcgtgct
aagcgtgccg ctaaaagtga gaaggattcg gctcccaccc 1440ccaaggttgc cctgccggtc
cccacctgtg gaattaccac ctactctcca ccgacagacg 1500ggtcttgtgg ttggcatgtc
cttgccgcca taatgaaccg gatgataaat ggtgacttca 1560cgtcccctct gactcagtac
aacagaccag aggatgattg ggcttctgat tatgatcttg 1620ttcaggcgat tcaatgtcta
cgactgcctg ctaccgtggt tcggaatcgc gcctgtccta 1680acgccaagta ccttataaaa
cttaacggag ttcactggga ggtagaggtg aggtctggaa 1740tggctcctcg ctccctttct
cgtgaatgtg tggttggcgt ttgctctgaa ggctgtgtcg 1800caccgcctta tccagcagac
gggctaccta aacgtgcact cgaggccttg gcgtctgctt 1860acagactacc ctccgattgt
gttagctctg gtattgctga ctttcttgct aatccacctc 1920ctcaggaatt ctggaccctc
gacaaaatgt tgacctcccc gtcaccagag cggtccggct 1980tctctagttt gtataaatta
ctattagagg ttgttccgca aaaatgcggt gccacggaag 2040gggctttcat ctatgctgtt
gagaggatgt tgaaggattg tccgagctcc aaacaggcca 2100tggcccttct ggcaaaaatt
aaagttccat cctcaaaggc cccgtctgtg tccctggacg 2160agtgtttccc tacggatgtt
ttagccgact tcgagccagc atctcaggaa aggccccaaa 2220gttccggcgc tgctgttgtc
ctgtgttcac cggatgcaaa agagttcgag gaagcagccc 2280cggaagaagt tcaagagagt
ggccacaagg ccgtccactc tgcactcctt gccgagggtc 2340ctaacaatga gcaggtacag
gtggttgccg gtgagcaact gaagctcggc ggttgtggtt 2400tggcagtcgg gaatgctcat
gaaggtgctc tggtctcagc tggtctaatt aacctggtag 2460gcgggaattt gtccccctca
gaccccatga aagaaaacat gctcaatagc cgggaagacg 2520aaccactgga tttgtcccaa
ccagcaccag cttccacaac gacccttgtg agagagcaaa 2580cacccgacaa cccaggttct
gatgccggtg ccctccccgt caccgttcga gaatttgtcc 2640cgacggggcc tatactctgt
catgttgagc actgcggcac ggagtcgggc gacagcagtt 2700cgcctttgga tctatctgat
gcgcaaaccc tggaccagcc tttaaatcta tccctggccg 2760cttggccagt gagggccacc
gcgtctgacc ctggctgggt ccacggtagg cgcgagcctg 2820tctttgtaaa gcctcgaaat
gctttctctg atggcgattc agcccttcag ttcggggagc 2880tttctgaatc cagctctgtc
atcgagtttg accggacaaa agatgctccg gtggttgacg 2940cccctgtcga cttgacgact
tcgaacgagg ccctctctgt agtcgatcct ttcgaatttg 3000ccgaactcaa gcgcccgcgt
ttctccgcac aagccttaat tgaccgaggc ggtccacttg 3060ccgatgtcca tgcaaaaata
aagaaccggg tatatgaaca gtgcctccaa gcttgtgagc 3120ccggtagtcg tgcaacccca
gccaccaggg agtggctcga caaaatgtgg gatagggtgg 3180acatgaaaac ttggcgctgc
acctcgcagt tccaagctgg tcgcattctt gcgtccctca 3240aattcctccc tgacatgatt
caagacacac cgcctcctgt tcccaggaag aaccgagcta 3300gtgacaatgc cggcctgaag
caactggtgg cacagtggga taggaaattg agtgtgaccc 3360cccccccaaa accggttggg
ccagtgcttg accagatcgt ccctccgcct acggatatcc 3420agcaagaaga tgtcaccccc
tccgatgggc caccccatgc gccggatttt cctagtcgag 3480tgagcacggg cgggagttgg
aaaggcctta tgctttccgg cacccgtctc gcggggtcta 3540tcagccagcg ccttatgaca
tgggtttttg aagttttctc ccacctccca gcttttatgc 3600tcacactttt ctcgccgcgg
ggctctatgg ctccaggtga ttggttgttt gcaggtgtcg 3660ttttacttgc tctcttgctc
tgtcgttctt acccgatact cggatgcctt cccttattgg 3720gtgtcttttc tggttctttg
cggcgtgttc gtctgggtgt ttttggttct tggatggctt 3780ttgctgtatt tttattctcg
actccatcca acccagtcgg ttcttcttgt gaccacgatt 3840cgccggagtg tcatgctgag
cttttggctc ttgagcagcg ccaactttgg gaacctgtgc 3900gcggccttgt ggtcggcccc
tcaggcctct tatgtgtcat tcttggcaag ttactcggtg 3960ggtcacgtta tctctggcat
gttctcctac gtttatgcat gcttgcagat ttggcccttt 4020ctcttgttta tgtggtgtcc
caggggcgtt gtcacaagtg ttggggaaag tgtataagga 4080cagctcctgc ggaggtggct
cttaatgtat ttcctttctc gcgcgccacc cgtgtctctc 4140ttgtatcctt gtgtgatcga
ttccaaacgc caaaaggggt tgatcctgtg cacttggcaa 4200cgggttggcg cgggtgctgg
cgtggtgaga gccccatcca tcaaccacac caaaagccca 4260tagcttatgc caatttggat
gaaaagaaaa tgtctgccca aacggtggtt gctgtcccat 4320acgatcccag tcaggctatc
aaatgcctga aagttctgca ggcgggaggg gccatcgtgg 4380accagcctac acctgaggtc
gttcgtgtgt ccgagatccc cttctcagcc ccatttttcc 4440caaaagttcc agtcaaccca
gattgcaggg ttgtggtaga ttcggacact tttgtggctg 4500cggttcgctg cggttactcg
acagcacaac tggttctggg ccggggcaac tttgccaagt 4560taaatcagac cccccccagg
aactctatct ccaccaaaac gactggtggg gcctcttaca 4620cccttgctgt ggctcaagtg
tctgcgtgga ctcttgttca tttcatcctc ggtctttggt 4680tcacatcacc tcaagtgtgt
ggccgaggaa ccgctgaccc atggtgttca aatccttttt 4740catatcctac ctatggcccc
ggagttgtgt gctcctctcg actttgtgtg tctgccgacg 4800gggtcaccct gccattgttc
tcagccgtgg cacaactctc cggtagagag gtggggattt 4860ttattttggt gctcgtctcc
ttgactgctt tggcccaccg catggctctt aaggcagaca 4920tgttagtggt cttttcggct
ttttgtgctt acgcctggcc catgagctcc tggttaatct 4980gcttctttcc tatactcttg
aagtgggtta cccttcaccc tcttactatg ctttgggtgc 5040actcattctt ggtgttttgt
ctgccagcag ccggcatcct ctcactaggg ataactggcc 5100ttctttgggc aattggccgc
tttacccagg ttgccggaat tattacacct tatgacatcc 5160accagtacac ctctgggcca
cgtggtgcag ctgctgtggc cacagcccca gaaggcactt 5220atatggccgc cgtccggaga
gctgctttaa ctgggcgaac tttaatcttc accccgtctg 5280cagttggatc ccttctcgaa
ggtgctttca ggactcataa accctgcctt aacaccgtga 5340atgttgtagg ctcttccctt
ggttccggag gggttttcac cattgatggc agaagaactg 5400tcgtcactgc tgcccatgtg
ttgaacggcg acacagctag agtcaccggc gactcctaca 5460accgcatgca cactttcaag
accaatggtg attatgcctg gtcccatgct gatgactggc 5520agggcgttgc ccctgtggtc
aaggttgcga aggggtaccg cggtcgtgcc tactggcaaa 5580catcaactgg tgtcgaaccc
ggtatcattg gggaagggtt cgccttctgt tttactaact 5640gcggcgattc ggggtcaccc
gtcatctcag aatctggtga tcttattgga atccacaccg 5700gttcaaacaa acttggttct
ggtcttgtga caacccctga aggggagacc tgcaccatca 5760aagaaaccaa gctctctgac
ctttccagac attttgcagg cccaagcgtt cctcttgggg 5820acattaaatt gagtccggcc
atcatccctg atgtaacatc cattccgagt gacttggcat 5880cgctcctagc ctccgtccct
gtagtggaag gcggcctctc gaccgttcaa cttttgtgtg 5940tctttttcct tctctggcgc
atgatgggcc atgcctggac acccattgtt gccgtgggct 6000tctttttgct gaatgaaatt
cttccagcag ttttggtccg agccgtgttt tcttttgcac 6060tctttgtgct tgcatgggcc
accccctggt ctgcacaggt gttgatgatt agactcctca 6120cggcatctct caaccgcaac
aagctttctc tggcgttcta cgcactcggg ggtgtcgtcg 6180gtttggcagc tgaaatcggg
acttttgctg gcagattgtc tgaattgtct caagctcttt 6240cgacatactg cttcttacct
agggtccttg ctatgaccag ttgtgttccc accatcatca 6300ttggtggact ccataccctc
ggtgtgattc tgtggttatt caaataccgg tgcctccaca 6360acatgctggt tggtgatggg
agtttttcaa gcgccttctt cctacggtat tttgcagagg 6420gtaatctcag aaaaggtgtt
tcacagtcct gtggcatgaa taacgagtcc ctaacggctg 6480ctttagcttg caagttgtca
caggctgacc ttgatttttt gtccagctta acgaacttca 6540agtgctttgt atctgcttca
aacatgaaaa atgctgccgg ccagtacatt gaagcagcgt 6600atgccaaggc cctgcgccaa
gagttggcct ctctagttca gattgacaaa atgaaaggag 6660ttttgtccaa gctcgaggcc
tttgctgaaa cagccacccc gtcccttgac ataggtgacg 6720tgattgttct gcttgggcaa
catcctcacg gatccatcct cgatattaat gtggggactg 6780aaaggaaaac tgtgtccgtg
caagagaccc ggagcctagg cggctccaaa ttcagtgttt 6840gtactgtcgt gtccaacaca
cccgtggacg ccttgaccgg catcccactc cagacaccaa 6900cccctctttt tgagaatggt
ccgcgtcatc gcagcgagga agacgatctt aaagtcgaga 6960ggatgaagaa acactgtgta
tccctcggct tccacaacat caatggcaaa gtttactgca 7020aaatttggga caagtctacc
ggtgacacct tttacacgga tgattcccgg tacacccaag 7080accatgcttt tcaggacagg
tcagccgact acagagacag ggactatgag ggtgtgcaaa 7140ccacccccca acagggattt
gatccaaagt ctgaaacccc tgttggcact gttgtgatcg 7200gcggtattac gtataacagg
tatctgatca aaggtaagga ggttctggtc cccaagcctg 7260acaactgcct tgaagctgcc
aagctgtccc ttgagcaagc tctcgctggg atgggccaaa 7320cttgcgacct tacagctgcc
gaggtggaaa agctaaagcg catcattagt caactccaag 7380gtttgaccac tgaacaggct
ttaaactgtt agccgccagc ggcttgaccc gctgtggccg 7440cggcggccta gttgtgactg
aaacggcggt aaaaattata aaataccaca gcagaacttt 7500caccttaggc cctttagacc
taaaagtcac ttccgaggtg gaggtaaaga aatcaactga 7560gcagggccac gctgttgtgg
caaacttatg ttccggtgtc atcttgatga gacctcaccc 7620accgtccctt gtcgacgttc
ttctgaaacc cggacttgac acaatacccg gcattcaacc 7680agggcatggg gccgggaata
tgggcgtgga cggttctatt tgggattttg aaaccgcacc 7740cacaaaggca gaactcgagt
tatccaagca aataatccaa gcatgtgaag ttaggcgcgg 7800ggacgccccg aacctccaac
tcccttacaa gctctatcct gttagggggg atcctgagcg 7860gcataaaggc cgccttatca
ataccaggtt tggagattta ccttacaaaa ctcctcaaga 7920caccaagtcc gcaatccacg
cggcttgttg cctgcacccc aacggggccc ccgtgtctga 7980tggtaaatcc acactaggta
ccactcttca acatggtttc gagctttatg tccctactgt 8040gccctatagt gtcatggagt
accttgattc acgccctgac acccctttta tgtgtactaa 8100acatggcact tccaaggctg
ctgcagagga cctccaaaaa tacgacctat ccacccaagg 8160atttgtcctg cctggggtcc
tacgcctagt acgcagattc atctttggcc atattggtaa 8220ggcgccgcca ttgttcctcc
catcaaccta tcccgccaag aactctatgg cagggatcaa 8280tggccagagg ttcccaacaa
aggacgttca gagcatacct gaaattgatg aaatgtgtgc 8340ccgcgctgtc aaggagaatt
ggcaaactgt gacaccttgc accctcaaga aacagtactg 8400ttccaagccc aaaaccagga
ccatcctggg caccaacaac tttattgcct tggctcacag 8460atcggcgctc agtggtgtca
cccaggcatt catgaagaag gcttggaagt ccccaattgc 8520cttggggaaa aacaaattca
aggagctgca ttgcactgtc gccggcaggt gtcttgaggc 8580cgacttggcc tcctgtgacc
gcagcacccc cgccattgta agatggtttg ttgccaacct 8640cctgtatgaa cttgcaggat
gtgaagagta cttgcctagc tatgtgctta attgctgcca 8700tgacctcgtg gcaacacagg
atggtgcctt cacaaaacgc ggtggcctgt cgtccgggga 8760ccccgtcacc agtgtgtcca
acaccgtata ttcactggta atttatgccc agcacatggt 8820attgtcggcc ttgaaaatgg
gtcatgaaat tggtcttaag ttcctcgagg aacagctcaa 8880gttcgaggac ctccttgaaa
ttcagcctat gttggtatac tctgatgatc ttgtcttgta 8940cgctgaaaga cccacatttc
ccaattacca ctggtgggtc gagcaccttg acctgatgct 9000gggtttcaga acggacccaa
agaaaaccgt cataactgat aaacccagct tcctcggctg 9060cagaattgag gcagggcgac
agctagtccc caatcgcgac cgcatcctgg ctgctcttgc 9120atatcacatg aaggcgcaga
acgcctcaga gtattatgcg tctgctgccg caatcctgat 9180ggattcatgt gcttgcattg
accatgaccc tgagtggtat gaggacctca tctgcggtat 9240tgcccggtgc gcccgccagg
atggttatag cttcccaggt ccggcatttt tcatgtccat 9300gtgggagaag ctgagaagtc
ataatgaagg gaagaaattc cgccactgcg gcatctgcga 9360cgccaaagcc gactatgcgt
ccgcctgtgg gcttgatttg tgtttgttcc attcgcactt 9420tcatcaacac tgccctgtca
ctctgagctg cggtcaccat gccggttcaa aggaatgttc 9480gcagtgtcag tcacctgttg
gggctggcag atcccctctt gatgccgtgc taaaacaaat 9540tccatacaaa cctcctcgta
ctgtcatcat gaaggtgggt aataaaacaa cggccctcga 9600tccggggagg taccagtccc
gtcgaggtct cgttgcagtc aagaggggta ttgcaggcaa 9660tgaagttgat ctttctgatg
gggactacca agtggtgcct cttttgccga cttgcaaaga 9720cataaacatg gtgaaggtgg
cttgcaatgt actactcagc aagttcatag tagggccacc 9780aggttccgga aagaccacct
ggctactgag tcaagtccag gacgatgatg tcatttacac 9840acccacccat cagactatgt
ttgatatagt cagtgctctc aaagtttgca ggtattccat 9900tccaggagcc tcaggactcc
ctttcccacc acctgccagg tccgggccgt gggttaggct 9960tattgccagc gggcacgtcc
ctggccgagt atcatacctc gatgaggctg gatattgtaa 10020tcatctggac attcttagac
tgctttccaa aacacccctt gtgtgtttgg gtgaccttca 10080gcaacttcac cctgtcggct
ttgattccta ctgttatgtg ttcgatcaga tgcctcagaa 10140gcagctgacc actatttaca
gatttggccc taacatctgc gcagccatcc agccttgtta 10200cagggagaaa cttgaatcta
aggctaggaa cactagggtg gtttttacca cccggcctgt 10260ggcctttggt caggtgctga
caccatacca taaagatcgc atcggctctg cgataaccat 10320agattcatcc cagggggcca
cctttgatat tgtgacattg catctaccat cgccaaagtc 10380cctaaataaa tcccgagcac
ttgtagccat cactcgggca agacacgggt tgttcattta 10440tgaccctcat aaccagctcc
aggagttttt caacttaacc cctgagcgca ctgattgtaa 10500ccttgtgttc agccgtgggg
atgagctggt agttctgaat gcggataatg cagtcacaac 10560tgtagcgaag gcccttgaga
caggtccatc tcgatttcga gtatcagacc cgaggtgcaa 10620gtctctctta gccgcttgtt
cggccagtct ggaagggagc tgtatgccac taccgcaagt 10680ggcacataac ctggggtttt
acttttcccc ggacagtcca acatttgcac ctctgccaaa 10740agagttggcg ccacattggc
cagtggttac ccaccagaat aatcgggcgt ggcctgatcg 10800acttgtcgct agtatgcgcc
caattgatgc ccgctacagc aagccaatgg tcggtgcagg 10860gtatgtggtc gggccgtcca
cctttcttgg tactcctggt gtggtgtcat actatctcac 10920actatacatc aggggtgagc
cccaggcctt gccagaaaca ctcgtttcaa cagggcgtat 10980agccacagat tgtcgggagt
atctcgacgc ggctgaggaa gaggcagcaa aagaactccc 11040ccacgcattc attggcgatg
tcaaaggtac cacggttggg gggtgtcatc acattacatc 11100aaaataccta cctaggtccc
tgcctaagga ctctgttgcc gtagttggag taagttcgcc 11160cggcagggct gctaaagccg
tgtgcactct caccgatgtg tacctccccg aactccggcc 11220atatctgcaa cctgagacgg
catcaaaatg ctggaaactc aaattagact tcagggacgt 11280ccgactaatg gtctggaaag
gagccaccgc ctatttccag ttggaagggc ttacatggtc 11340ggcgctgccc gactatgcca
ggtttattca gctgcccaag gatgccgttg tatacattga 11400tccgtgtata ggaccggcaa
cagccaaccg taaggtcgtg cgaaccacag actggcgggc 11460cgacctggca gtgacaccgt
atgattacgg tgcccagaac attttgacaa cagcctggtt 11520cgaggacctc gggccgcagt
ggaagatttt ggggttgcag ccctttaggc gagcatttgg 11580ctttgaaaac actgaggatt
gggcaatcct tgcacgccgt atgaatgacg gcaaggacta 11640cactgactat aactggaact
gtgttcgaga acgcccacac gccatctacg ggcgtgctcg 11700tgaccatacg tatcattttg
cccctggcac agaattgcag gtagagctag gtaaaccccg 11760gctgccgcct gggcaagtgc
cgtgaattcg gggtgatgca atggggtcac tgtggagtaa 11820aatcagccag ctgttcgtgg
acgccttcac tgagttcctt gttagtgtgg ttgatattgc 11880cattttcctt gccatactgt
ttgggttcac cgtcgcagga tggttactgg tctttcttct 11940cagagtggtt tgctccgcgc
ttctccgttc gcgctctgcc attcactctc ccgaactatc 12000gaaggtccta tgaaggcttg
ttgcccaact gcagaccgga tgtcccacaa tttgcagtca 12060agcacccatt gggtatgttt
tggcacatgc gagtttccca cttgattgat gagatggtct 12120ctcgtcgcat ttaccagacc
atggaacatt caggtcaagc ggcctggaag caggtggttg 12180gtgaggccac tctcacgaag
ctgtcagggc tcgatatagt tactcatttc caacacctgg 12240ccgcagtgga ggcggattct
tgccgctttc tcagctcacg actcgtgatg ctaaaaaatc 12300ttgccgttgg caatgtgagc
ctacagtaca acaccacgtt ggaccgcgtt gagctcatct 12360tccccacgcc aggtacgagg
cccaagttga ccgatttcag acaatggctc atcagtgtgc 12420acgcttccat tttttcctct
gtggcttcat ctgttacctt gttcatagtg ctttggcttc 12480gaattccagc tctacgctat
gtttttggtt tccattggcc cacggcaaca catcattcga 12540gctgaccatc aactacacca
tatgcatgcc ctgttctacc agtcaagcgg ctcgccaaag 12600gctcgagccc ggtcgtaaca
tgtggtgcaa aatagggcat gacaggtgtg aggagcgtga 12660ccatgatgag ttgttaatgt
ccatcccgtc cgggtacgac aacctcaaac ttgagggtta 12720ttatgcttgg ctggcttttt
tgtccttttc ctacgcggcc caattccatc cggagttgtt 12780cgggataggg aatgtgtcgc
gcgtcttcgt ggacaagcga caccagttca tttgtgccga 12840gcatgatgga cacaattcaa
ccgtatctac cggacacaac atctccgcat tatatgcggc 12900atattaccac caccaaatag
acgggggcaa ttggttccat ttggaatggc tgcggccact 12960cttttcttcc tggctggtgc
tcaacatatc atggtttctg aggcgttcgc ctgtaagccc 13020tgtttctcga cgcatctatc
agatattgag accaacacga ccgcggctgc cggtttcatg 13080gtccttcagg acatcaattg
tttccgacct cacggggtct cagcagcgca agagaaaatt 13140tccttcggaa agtcgtccca
atgtcgtgaa gccgtcggta ctccccagta catcacgata 13200acggctaacg tgaccgacga
atcatacttg tacaacgcgg acctgctgat gctttctgcg 13260tgccttttct acgcctcaga
aatgagcgag aaaggcttca aagtcatctt tgggaatgtc 13320tctggcgttg tttctgcttg
tgtcaatttc acagattatg tggcccatgt gacccaacat 13380acccagcagc atcatctggt
aattgatcac attcggttgc tgcatttcct gacaccatct 13440gcaatgaggt gggctacaac
cattgcttgt ttgttcgcca ttctcttggc aatatgagat 13500gttctcacaa attggggcgt
ttcttgactc cgcactcttg cttctggtgg ctttttttgc 13560tgtgtaccgg cttgtcctgg
tcctttgccg atggcaacgg cgacagctcg acataccaat 13620acatatataa cttgacgata
tgcgagctga atgggaccga ctggttgtcc agccattttg 13680gttgggcagt cgagaccttt
gtgctttacc cggttgccac tcatatcctc tcactgggtt 13740ttctcacaac aagccatttt
tttgacgcgc tcggtctcgg cgctgtatcc actgcaggat 13800ttgttggcgg gcggtacgta
ctctgcagcg tctacggcgc ttgtgctttc gcagcgttcg 13860tatgttttgt catccgtgct
gctaaaaatt gcatggcctg ccgctatgcc cgtacccggt 13920ttaccaactt cattgtggac
gaccggggga gagttcatcg atggaagtct ccaatagtgg 13980tagaaaaatt gggcaaagcc
gaagtcgatg gcaacctcgt caccatcaaa catgtcgtcc 14040tcgaaggggt taaagctcaa
cccttgacga ggacttcggc tgagcaatgg gaggcctaga 14100cgatttttgc aacgatccta
tcgccgcaca aaagctcgtg ctagccttta gcatcacata 14160cacacctata atgatatacg
cccttaaggt gtcacgcggc cgactcctgg ggctgttgca 14220catcctaata tttctgaact
gttcctttac attcggatac atgacatatg tgcattttca 14280atccaccaac cgtgtcgcac
ttaccctggg ggctgttgtc gcccttctgt ggggtgttta 14340cagcttcaca gagtcatgga
agtttatcac ttccagatgc agattgtgtt gccttggccg 14400gcgatacatt ctggcccctg
cccatcacgt agaaagtgct gcaggtctcc attcaatctc 14460agcgtctggt aaccgagcat
acgctgtgag aaagcccgga ctaacatcag tgaacggcac 14520tctagtacca ggacttcgga
gcctcgtgct gggcggcaaa cgagctgtta aacgaggagt 14580ggttaacctc gtcaagtatg
gccggtaaaa accagagcca gaagaaaaag aaaagtacag 14640ctccgatggg gaatggccag
ccagtcaatc aactgtgcca gttgctgggt gcaatgataa 14700agtcccagcg ccagcaacct
aggggaggac aggccaaaaa gaaaaagcct gagaagccac 14760attttcccct ggctgctgaa
gatgacatcc ggcaccacct cacccagact gaacgctccc 14820tctgcttgca atcgatccag
acggctttca atcaaggcgc aggaactgcg tcgctttcat 14880ccagcgggaa ggtcagtttt
caggttgagt ttatgctgcc ggttgctcat acagtgcgcc 14940tgattcgcgt gacttctaca
tccgccagtc agggtgcaag ttaatttgac agtcaggtga 15000atggccgcga ttggcgtgtg
gcctctgagt cacctattca attagggcga tcacatgggg 15060gtcatactta atcaggcagg
aaccatgtga ccgaaattaa aaaaaaaaaa a 151111515182DNAPorcine
reproductive and respiratory syndrome virus 15tttctccacc cctttaacca
tgtctgggat acttgatcgg tgcacgtgta cccccaatgc 60cagggtgttt atggcggagg
gccaagtcta ctgcacacga tgcctcagtg cacggtctct 120ccttcccctg aacctccaag
tttctgagct cggggtgcta ggcctattct acaggcccga 180agagccactc cggtggacgt
tgccacgtgc attccccact gttgagtgct cccccgccgg 240ggcctgctgg ctctctgcaa
tctttccaat cgcacgaatg accagtggaa acctgaactt 300ccaacaaaga atgatacggg
tcgcagctga gctttacaga gccggccagc tcacccctgc 360agtcttgaag gctctacaag
tttatgaacg gggttgccgc tggtacccca ttgttggacc 420tgcccctgga gtggccgttt
acgccaattc cctacatgtg agtgataaac ctttcccggg 480agcaactcac gtgttgacca
acctgccgct cccgcagaga cccaagcctg aagacttttg 540cccctttgag tgtgctatgg
ctactgtcta tgacattggt catgacgccg tcatgtatgt 600ggccgaaagg aaaatctcct
gggcccctcg tggcggggat gaagtgaaat ttgaagctgt 660ccccggggag ttgaggttga
ttgcgaaccg gctccgcacc tccttcccgc cccaccacac 720agtggacatg tctaagttcg
ccttcacagc ccctgggtgt ggtgtttcta tgcgggttga 780acgccaacac ggctgccttc
ccgctgacac tgtccctgaa ggcaactgct ggtggagctt 840gtttgactcg cttccactgg
aagttcagaa caaagaaatt cgccatgcta accaatttgg 900ctaccagacc aagcatggtg
tctctggcaa gtacctacag cggaggctgc aagttaatgg 960tctccgagca gtaactgacc
taaacggacc tatcgtcgta cagtacttct ccgttaagga 1020gagttggatc cgccatttga
aactggcggg agaacccagc tactctgggt ttgaggacct 1080cctcagaata agggttgagc
ctaacacgtc gccattggct gacaaggaag aaaaaatttt 1140ccggtttggc agtcacaagt
ggtacggcgc tggaaagaga gcaagaaaag cacgctcttg 1200tgcgactgcc acagtcgctg
gccgcgcttt gtccgttcgt gaaacccggc aggccaagga 1260gcacgaggtt gccggcgcca
acaaggctga gcacctcaaa cactactccc cgcctgccga 1320agggaattgt ggttggcatt
gcatttccgc catcgccaac cggatggtga attccaaatt 1380tgaaaccacc cttcccgaaa
gagtgagacc tccagatgac tgggctactg acgaggatct 1440tgtgaatgcc atccaaatcc
tcagactccc tgcggcctta gacaggaacg gtgcttgtac 1500tagcgccaag tacgtactta
agctggaagg tgagcattgg actgtcactg tggcccctgg 1560gatgtcccct tctttgctcc
ctcttgaatg tgttcagggc tgttgtgggc acaagggcgg 1620tcttggttcc ccagatgcag
tcgaggtctc cggatttgac cctgcctgcc ttgaccggct 1680ggctgaggtg atgcacctgc
ctagcagtgc tatcccagcc gctctggccg aaatgtctgg 1740cgattccgat cgttcggctt
ctccggtcac caccgtgtgg actgtttcgc agttctttgc 1800ccgtcacagc ggagggaatc
accctgatca agtgcgctta gggaaaatta tcagcctttg 1860tcaggtgatt gaggactgct
gctgttccca gaacaaaacc aaccgggtca ccccggagga 1920ggtcgcagca aagattgacc
tgtacctccg tggtgcaaca aatcttgaag aatgcttggc 1980caggcttgag aaagcgcgcc
cgccacgcgt aatcgacacc ttctttgatt gggatgttgt 2040gctccctggg gttgaggcgg
caacccagac gatcaagctg ccccaggtca accagtgtcg 2100tgctctggtc cctgttgtga
ctcaaaagtc cttggacgac aactcggtcc ccctgaccgc 2160cttttcactg gctaactact
actaccgtgc gcaaggtgac gaagttcgtc accgtgaaag 2220actaaccgcc gtgctctcca
agttggaaaa ggttgttcga gaagaatatg ggctcatgcc 2280aaccgagcct ggtccacggc
ccacactgcc acgcgggctc gacgaactca aagaccagat 2340ggaggaggac ttgctgaagc
tggctaacgc ccagacgact tcggacatga tggcctgggc 2400agtcgagcag gttgacctaa
aaacttgggt caagaactac ccgcggtgga caccaccacc 2460ccctccgcca aaagttcagc
ctcgaaaaac gaagcctgtc aagagcttgc cggagagaaa 2520gcctgtcccc gccccgcgca
ggaaggttgg gtccgattgt ggcagcccgg tttcattagg 2580cggcgatgtc tctaacagtt
gggaagattt ggctgttagt agcccctttg atctcccgac 2640cccacctgag ccggcaacac
cttcaagtga gctggtgatt gtgtcctcac cgcaatgcat 2700cttcaggccg gcgacaccct
tgagtgagcc ggctccaatt cccgcacctc gcggaactgt 2760gtctcgaccg gtgacaccct
tgagtgagcc gatccctgtg cccgcaccgc ggcgtaagtt 2820tcagcaggtg aaaagattga
gttcggcggc ggcaatccca ccgtaccaga acgagcccct 2880ggatttgtct gcttcctcac
agactgaaca tgaggcctct cccccagcac cgccgcagag 2940cgggggcgtt ccgggagtag
aggggcatga agctgaggaa accctgagtg aaatctcgga 3000catgtcgggt aacattaaac
ctgcgtccgt gtcatcaagc agctccttgt ccagcgtgag 3060aatcacacgc ccaaaatact
cagctcaagc catcatcgac tcgggcgggc cctgcagtgg 3120gcatctccaa gaggtaaagg
aaacatgcct tagtgtcatg cgcgaggcat gtgatgcgac 3180taagcttgat gaccctgcta
cgcaggaatg gctttctcgc atgtgggatc gggtggacat 3240gctgacttgg cgcaacacgt
ctgtttacca ggcgatttgc accttagatg gcaggttaaa 3300gttcctccca aaaatgatac
tcgagacacc gccgccctat ccgtgtgagt ttgtgatgat 3360gcctcacacg cctgcacctt
ccgtaggtgc ggagagcgac cttaccattg gctcagttgc 3420tactgaagat gttccacgca
tcctcgagaa aatagaaaat gtcggcgaga tggccaacca 3480gggacccttg gccttctccg
aggataaacc ggtagatgac caacttgtca acgacccccg 3540gatatcgtcg cggaggcctg
acgagagcac atcagctccg tccgcaggca caggtggcgc 3600cggctctttt accgatttgc
cgccttcaga tggcgcggat acggacgggg gggggccgtt 3660tcggacggca aaaagaaaag
ctgaaaggct ctttgaccaa ctgagccgtc aggtttttga 3720cctcgtctcc catctccctg
ttttcttctc acgccttttc taccctggcg gtggttattc 3780tccgggtgat tggggttttg
cagcttttac tctattgtgc ctctttttat gttacagtta 3840cccagccttt ggtattgctc
ccctcttggg tgtgttttct gggtcttctc ggcgcgttcg 3900aatgggggtt tttggctgct
ggttggcttt tgctgttggt ctgttcaagc ctgtgtccga 3960cccagtcggc gctgcttgtg
agtttgactc gccagagtgt agaaacatcc ttcattcttt 4020tgagcttctc aaaccttggg
accctgttcg cagccttgtt gtgggccccg tcggtctcgg 4080tcttgccatt cttggcaggt
tactgggcgg ggcacgctgc atctggcact ttttgcttag 4140gcttggcatt gttgcagact
gtatcttggc tggagcttac gtgctttctc aaggtaggtg 4200taaaaagtgc tggggatctt
gtataagaac tgctcctaat gaggtcgctt ttaacgtgtt 4260tcctttcaca cgtgcgacca
ggtcgtcact tatcgacctg tgcgatcggt tttgtgcgcc 4320aaaaggaatg gaccccattt
ttctcgccac tgggtggcgc gggtgctggg ccggccgaag 4380ccccattgag caaccctctg
aaaaacccat cgcgtttgcc caattggatg aaaagaagat 4440tacggctagg actgtggtcg
cccagcctta tgaccccaac caagccgtaa agtgcttgcg 4500ggtattgcag gcgggtgggg
cgatggtggc taaggcggtc ccaaaagtgg ttaaggtttc 4560cgctgttcca ttccgagctc
ccttctttcc cactggagtg aaagttgacc ctgattgcag 4620ggtcgtggtt gaccctgaca
ctttcactgc agctctccgg tctggctact ccaccacaaa 4680cctcgtcctt ggtgtagggg
actttgccca gctaaatgga ttaaaaatca ggcaaatttc 4740caagccttca gggggaggcc
cacatctcat ggctgccctg catgttgcct gctcgatggc 4800tctgcacatg cttgctggga
tttatgtgac tgcggtgggt tcttgcggca ccggcaccaa 4860cgacccgtgg tgcgctaacc
cgtttgccgt ccctggctac ggacctggct ctctctgtac 4920gtccagattg tgcatttccc
aacacggcct taccctgccc ttgtcagcac ttgtggcggg 4980attcggtatt caagaaattg
ccttggtcgt tttgattttt gtttccatcg gaggcatggc 5040tcataggttg agctgtaagg
ctgacatgct gtgtgttttg cttgcaattg ccagctatgt 5100ttgggtacct cttacctggt
tgctttgtgt gtttccttgc tggttgcgct gtttttcttt 5160gcaccccctc accatcctat
ggttggtgtt tttcttgatt tctgtgaata tgccttcagg 5220aatcttggcc atggtgttgt
tggtttctct ttggcttctt ggtcgttata ctaatgttgc 5280tggccttgtc accccctacg
acattcatca ttacaccagt ggcccccgcg gtgttgccgc 5340cttggctacc gcaccagatg
ggacctactt ggccgctgtc cgccgcgctg cgttgactgg 5400ccgcaccatg ctgtttaccc
cgtcccagct tgggtctctt cttgagggtg ccttcagaac 5460tcgaaagccc tcactgaaca
ccgtcaatgt gatcgggtcc tccatgggct ctggcggggt 5520gtttaccatc gacgggaaag
tcaagtgcgt aactgccgca catgtcctta cgggcaattc 5580agctcgggtt tccggggtcg
gcttcaatca aatgcttgac tttgacgtaa agggagattt 5640cgctatcgct gattgcccga
attggcaagg ggctgccccc aagacccaat tctgcacgga 5700tggatggact ggccgtgcct
attggctaac atcctctggc gtcgaacccg gcgtcattgg 5760aaaaggattc gccttctgct
tcaccgcatg tggcgattcc gggtccccag tgatcaccga 5820ggccggtgag cttgtcggcg
ttcacacggg atcgaataaa caaggggggg gcattgttac 5880gcgcccctca ggccagtttt
gtaatgtggc acccatcaag ctaagcgaat taagtgaatt 5940ctttgctggg cctaaggtcc
cgctcggtga tgtgaaggtc ggcagccaca taattaaaga 6000cataagcgag gtgccttcag
atctttgtgc cttgcttgct gccaaacctg aactggaagg 6060aggcctctcc accgtccaac
ttctttgtgt gttttttctc ctgtggagaa tgatgggaca 6120tgcctggacg cccttggttg
ctgtgagttt ctttattttg aatgaggttc tccctgccgt 6180cctggtccgg agtgttttct
cctttggaat gtttgtgcta tcctggctca cgccatggtc 6240tgcgcaagtt ctgatgatca
ggcttctgac agcagctctt aacaggaaca gatggtcact 6300tgcctttttc agcctcggtg
cagtgaccgg ttttgtcgca gatcttgcgg ccactcaggg 6360gcatccgttg caggcagtga
tgaatttgag cacctatgca ttcctgcctc ggatgatggt 6420tgtgacctca ccagtcccag
tgatcacgtg tggtgtcgtg cacctacttg ccatcatttt 6480gtacttgttt aagtaccgtg
gcctgcacca tatccttgtt ggcgatggag tgttctctgc 6540ggctttcttc ttgagatact
ttgccgaggg aaagttgagg gaaggggtgt cgcaatcctg 6600cggaatgaat catgagtctc
tgactggtgc cctcgctatg agactcaatg acgaggactt 6660ggatttcctt atgaaatgga
ctgattttaa gtgctttgtt tctgcgtcca acatgaggaa 6720tgcagcgggt caatttatcg
aggctgccta tgctaaagca cttagagtag aactggccca 6780gttggtacag gttgataaag
ttcgaggtac tttggccaaa cttgaagctt ttgctgatac 6840cgtggctcct caactctcgc
ccggtgacat tgttgtcgct ctcggccaca cgcctgttgg 6900cagtatcttc gacctaaagg
ttggtagcac caagcatacc ctccaagcca ttgagaccag 6960agtccttgct gggtccaaaa
tgaccgtggc gcgcgtcgtc gacccgaccc ccacgccccc 7020acccgcaccc gtgcccatcc
ccctcccacc gaaagttctg gagaatggcc ccaacgcttg 7080gggggatgag gaccgtttga
ataagaagaa gaggcgcagg atggaagccc tcggcatcta 7140tgttatgggc gggaaaaagt
accagaaatt ttgggacaag aattccggtg atgtgtttta 7200tgaggaggtc cataataaca
cagatgagtg ggagtgtctc agagttggcg accctgccga 7260ctttgaccct gagaagggaa
ctctgtgtgg acatgtcacc attgaaaata aggcttacca 7320tgtttacacc tccccatctg
gtaagaagtt cttggtcccc gtcaacccag agaatggaag 7380agttcaatgg gaagctgcaa
agctttccgt ggagcaggcc ctaggtatga tgaatgtcga 7440cggcgaactg actgccaaag
aactggagaa actgaaaaga ataattgaca aactccaggg 7500cctgactaag gagcagtgtt
taaactgcta gccgccagcg acttgacccg ctgtggtcgc 7560ggcggcttgg ttgttactga
aacagcggta aaaatagtca aatttcacaa ccggaccttc 7620accctgggac ctgtgaattt
aaaagtggcc agtgaggttg agctaaaaga cgcggttgag 7680cacaaccaac acccggttgc
gagaccgatc gatggtggag ttgtgctctt gcgttccgcg 7740gttccttcgc ttatagacgt
cttgatctcc ggtgctgatg catctcccaa gttacttgcc 7800catcacgggc cgggaaacac
tgggatcgat ggcacgctct gggattttga gtccgaagcc 7860actaaagagg aagtcgcact
cagtgcgcaa ataatacagg cttgtgacat taggcgcggc 7920gacgctcctg aaattggtct
cccttacaag ctgtaccctg ttaggggtaa ccctgagcgg 7980gtgaaaggag ttctgcagaa
tacaaggttt ggagacatac cttacaaaac ccccagtgac 8040actggaagcc cagtgcacgc
ggctgcctgc cttacgccca acgccactcc ggtgactgat 8100gggcgctccg tcttggccac
gaccatgccc cccgggtttg agttatatgt accgaccata 8160ccagcgtctg tccttgatta
ccttgactct aggcctgact gccctaaaca gctgacagag 8220cacggctgcg aagatgccgc
actgaaagac ctctctaaat atgacttgtc cacccaaggc 8280tttgttttac ctggagttct
tcgccttgtg cggaaatacc tgtttgccca tgtaggtaag 8340tgcccacccg ttcatcggcc
ttctacttac cctgctaaga attctatggc tggaataaat 8400gggaacaggt tcccaaccaa
ggacattcag agcgtccctg aaatcgacgt tctgtgcgca 8460caggctgtgc gagaaaactg
gcaaactgtc accccttgta ctcttaagaa acagtattgc 8520gggaagaaga agactaggac
catactcggc accaataact tcatcgcact agcccaccga 8580gcagtgttga gtggtgttac
ccagggcttc atgaaaaagg cgtttaactc gcccatcgcc 8640ctcggaaaga acaagtttaa
ggagctacag actccggtcc tgggcaggtg ccttgaagct 8700gatctcgcat cctgcgatcg
atccacgcct gcaattgtcc gctggtttgc cgccaacctt 8760ctttatgaac ttgcctgtgc
tgaagagcat ctaccgtcgt acgtgctgaa ctgctgccac 8820gacttactgg tcacgcagtc
cggcgcagtg actaagagag gtggcctgtc gtctggcgac 8880ccgatcacct ctgtgtctaa
caccatttat agtttggtga tctatgcaca gcatatggtg 8940cttagttact tcaaaagtgg
tcacccccat ggccttctgt tcttacaaga ccagctaaag 9000tttgaggaca tgctcaaggt
tcaacccctg atcgtctatt cggacgacct cgtgctgtat 9060gccgagtctc ccaccatgcc
aaactatcac tggtgggttg aacatctgaa tttgatgctg 9120gggtttcaga cggacccaaa
gaagacagca ataacagact cgccatcatt tctaggctgt 9180agaataataa atgggcgcca
gctagtcccc aaccgtgaca ggatcctcgc ggccctcgcc 9240tatcacatga aggcgagtaa
tgtttctgaa tactatgcct cagcggctgc aatactcatg 9300gacagctgtg cttgtttgga
gtatgatcct gaatggtttg aagaacttgt agttggaata 9360gcgcagtgcg cccgcaagga
cggctacagt tttcccggca cgccgttctt catgtccatg 9420tgggaaaaac tcaggtccaa
ttatgagggg aagaagtcga gagtgtgcgg gtactgcggg 9480gccccggccc cgtacgctac
tgcctgtggc ctcgacgtct gcatttacca cacccacttc 9540caccagcatt gtccagtcac
aatctggtgt ggccatccag cgggttctgg ttcttgtagt 9600gagtgcaaat cccctgtagg
gaaaggcaca agccctttag acgaggtgct ggaacaagtc 9660ccgtataagc ccccacggac
cgttatcatg catgtggagc agggtctcac cccccttgat 9720ccaggtagat accaaactcg
ccgcggacta gtctctgtca ggcgtggaat taggggaaat 9780gaagttgaac taccagacgg
tgattatgct agcaccgcct tgctccctac ctgcaaagag 9840atcaacatgg tcgctgtcgc
ttccaatgta ctgcgcagca ggttcatcat cggcccaccc 9900ggtgctggga aaacatactg
gctccttcaa caggtccagg atggtgatgt tatttacaca 9960ccaactcacc agaccatgct
tgacatgatt agggctttgg ggacgtgccg gttcaacgtc 10020ccggcaggca caacgctgca
attccccgtc ccctcccgca ccggtccgtg ggttcgcatc 10080ctagccggcg gttggtgtcc
tggcaagaat tccttcctag atgaagcagc gtattgcaat 10140caccttgatg ttttgaggct
tcttagtaaa actaccctca cctgtctagg agacttcaag 10200caactccacc cagtgggttt
tgattctcat tgctatgttt ttgacatcat gcctcaaact 10260caactgaaga ccatctggag
gtttggacag aatatctgtg atgccgttca gccagattac 10320agggacaaac tcatgtccat
ggtcaacaca acccgtgtga cctacgtgga aaaacctgtc 10380aggtatgggc aggtcctcac
cccctaccac agggaccgag aggacgacgc catcactatt 10440gactccagtc aaggcgccac
attcgatgtg gttacattgc atttgcccac taaagattca 10500ctcaacaggc aaagagccct
tgttgccatc accagggcaa gacacgctat ctttgtgtat 10560gacccacaca ggcagctgca
gggcttgttt gatcttcctg caaaaggcac acccgtcaac 10620ctcgcagtgc accgcgacgg
gcagctgatc gtgctggata gaaataacaa agaatgcacg 10680gtcgctcagg ctctaggcaa
cggggataaa tttagggcca cagataagcg tgttgtagat 10740tctctccgcg ccatttgtgc
tgatctagaa gggtcgagct ctccgctccc caaggtcgca 10800cacaacttgg gattttattt
ctcacctgat ttaacacagt ttgctaaact cccagtagaa 10860cttgcacctc actggcccgt
ggtgacaacc cagaacaatg aaaagtggcc agatcggctg 10920gttgccagcc ttcgccctat
ccataaatac agccgcgcgt gcatcggtgc cggctatatg 10980gtgggccctt cggtgtttct
aggcactcct ggggtcgtgt catactatct cacaaaattt 11040gttaagggcg aggctcaatt
gcttccggag acggttttca gcaccggccg aattgaggta 11100gactgccggg aatatcttga
tgatcgggag cgagaagttg ctgcgtccct cccacacgct 11160ttcattggcg acgtcaaagg
cactaccgtt ggaggatgtc atcatgtcac ctccagatac 11220ctcccacgcg tccttcccaa
ggaatcagtt gcggtagtcg gggtttcaag ccccggaaaa 11280gccgcgaaag cattgtgcac
actgacagat gtgtacctcc cagatcttga cgcctatctc 11340cacccggaga cccagtccaa
gtgctggaaa atgatgttgg acttcaaaga agttcgacta 11400atggtctgga aagacaaaac
agcctatttc caacttgaag gtcgctattt cacctggtat 11460cagcttgcca gctatgcctc
gtacatccgt gttcctgtca actctacggt gtacttggac 11520ccctgcatgg gccccgccct
ttgcaacagg agagtcgtcg ggtccaccca ctggggggct 11580gacctcgcgg tcacccctta
tgattacggc gctaaaatta tcctgtctag cgcgtaccat 11640ggtgaaatgc cccccggata
caaaattctg gcgtgcgcgg agttctcgtt ggatgaccca 11700gttaagtaca aacatacctg
ggggtttgaa tcggatacag cgtatctgta tgagttcacc 11760ggaaacggtg aggactggga
ggattacaat gatgcgtttc gtgcgcgcca ggaagggaaa 11820atttataagg ccactgccac
cagcttgaag ttttattttc ccccgggccc tgtcattgaa 11880ccaactttag gcctgaattg
aaatgaaatg gggtccatgc aaagcctttt ttacaaaatt 11940ggccaacttt ttgtggatgc
tttcacggag ttcttggtgt ccattgttga tatcactata 12000tttttggcca ttttgtttgg
cttcaccatc gccggttggc tggtggtctt ttgcatcaga 12060ttggtttgct ccgcgatact
ccgtacgcgc tctgccattc actctgagca attacagaag 12120atcttatgag gcctttcttt
cccagtgcca agtggacatt cccacctggg gaactaaaca 12180tcctttgggg atgctttggc
accataaggt gtcaaccctg attgatgaaa tggtgtcgcg 12240tcgaatgtac cgcatcatgg
aaaaagcagg gcaggctgcc tggaaacagg tggtgagcga 12300ggctacgctg tctcgcatta
gtagtttgga tgtggtggct cattttcagc atctagccgc 12360cattgaagcc gagacctgta
aatatttggc ctcccggctg cccatgctac acaacctgcg 12420catgacaggt tcaaatgtaa
ccatagtgta taatagcact ttgaatcagg tgtttgctat 12480ttttccaacc cctggttccc
ggccaaagct taatgatttt cagcaatggt taatagctgt 12540acattcctcc atattttcct
ctgttgcaac ttcttgtact ctttttgttg tgctgtggtt 12600gcgggttcca atactacgta
ctgcttttgg tttccgctgg ttaggggcaa tttttctttc 12660gaactcacag tgaattacac
ggtgtgtcca ccttgcctca cccggcaagc agccgcagag 12720atctacgaac ccggtaggtc
tctttggtgc aggatagggt atgaccgatg tgaggaggat 12780gatcatgacg agctagggtt
tatggtaccg cctggcctct ccagcgaagg ccacttgact 12840agtgtttacg cctggttggc
gttcttgtcc ttcagctaca cggcccagtt ccatcccgag 12900atattcggga tagggaatgt
gagtcgagtt tatgttgaca tcaaacatca actcatctgc 12960gccgaacatg acgggcagaa
caccaccttg cctcgtcatg acaacatttc agccgtgttt 13020cagacctatt accaacatca
agtcgacggc ggcaattggt ttcacctaga atggcttcgt 13080cccttctttt cctcgtggtt
ggttttaaat gtctcttggt ttctcaggcg ttcgcctgca 13140aaccatgttt cagttcgagt
cttgcagata ttaagaccaa caccaccgca gcggcaagct 13200ttgctgtcct ccaagacatc
agttgcctta ggcatcgcga ctcggcctct gaggcgattc 13260gcaaaatccc tcagtgccgt
acggcgatag ggacacccgt gtatgttacc atcacagcca 13320atgtgacaga tgagaattat
ttacattctt ctgatctcct catgctttct tcttgccttt 13380tctatgcttc tgagatgagt
gaaaagggat ttaaggtggt atttggcaat gtgtcaggca 13440tcgtggctgt gtgtgtcaat
tttaccagct acgtccaaca tgtcaaggag tttacccaac 13500gctccctggt ggtcgaccat
gtgcggttgc tccatttcat gacacctgag accatgaggt 13560gggcaactgt tttagcctgt
ctttttgcca ttctgttggc aatttgaatg tttaagtatg 13620ttggagaaat gcttgaccgc
gggctgttac tcgcaattgc tttctttgtg gtgtatcgtg 13680ccgttctgtt ttgctgtgct
cgtcaacgcc agcaacgaca gcagctccca tctacagctg 13740atttacaact tgacgctatg
tgagctgaat ggcacagatt ggctagctaa caaatttgat 13800tgggcagtgg agagttttgt
catctttccc gttttgactc acattgtctc ctatggtgcc 13860ctcactacta gccatttcct
tgacacagtc gctttagtca ctgtgtctac cgccgggttt 13920gttcacgggc ggtatgtcct
aagtagcatc tacgcggtct gtgccctggc tgcgttgact 13980tgcttcgtca ttaggtttgc
aaagaattgc atgtcctggc gctacgcgtg taccagatat 14040accaactttc ttctggacac
taagggcaga ctctatcgtt ggcggtcgcc tgtcatcata 14100gagaaaaggg gcaaagttga
ggtcgaaggt catctgatcg acctcaaaag agttgtgctt 14160gatggttccg tggcaacccc
tataaccaga gtttcagcgg aacaatgggg tcgtccttag 14220atgacttctg tcatgatagc
acggctccac aaaaggtgct tttggcgttt tctattacct 14280acacgccagt gatgatatat
gccctaaagg tgagtcgcgg ccgactgcta gggcttctgc 14340accttttgat cttcctgaat
tgtgctttca ccttcgggta catgactttc gcgcactttc 14400agagtacaaa taaggtcgcg
ctcactatgg gagcagtagt tgcactcctt tggggggtgt 14460actcagccat agaaacctgg
aaattcatca cctccagatg ccgtttgtgc ttgctaggcc 14520gcaagtacat tctggcccct
gcccaccacg ttgaaagtgc cgcaggcttt catccgattg 14580cggcaaatga taaccacgca
tttgtcgtcc ggcgtcccgg ctccactacg gtcaacggca 14640cattggtgcc cgggttaaaa
agcctcgtgt tgggtggcag aaaagctgtt aaacagggag 14700tggtaaacct tgtcaaatat
gccaaataac aacggcaagc agcagaatag aaagaagggg 14760gatggccagc cagtcaatca
gctgtgccag atgctgggta agatcatcgc tcagcaaaac 14820cagtccagag gcaagggacc
gggaaagaaa aataagaaga aaaacccgga gaagccccat 14880tttcctctag cgactgaaga
tgatgtcaga catcacttta cccctagtga gcggcaattg 14940tgtctgtcgt caatccagac
cgcctttaat caaggcgctg ggacttgcac cctgtcagat 15000tcagggagga taagttacac
tgtggagttt agtttgccta cgcatcatac tgtgcgcctg 15060attcgcgtca cagcatcacc
ctcagcatga tgggctggca ttcttgaggc atctcagtgt 15120ttgaattgga agaatgtgtg
gtgaatggca ctgattgaca ttgtgcctct aagcactata 15180tt
1518216383PRTPorcine
reproductive and respiratory syndrome virus 16Met Ser Gly Thr Phe Ser Arg
Cys Met Cys Thr Pro Ala Ala Arg Val 1 5
10 15 Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg
Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile
Gly 35 40 45 Leu
Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50
55 60 Ile Pro Gln Val Glu Cys
Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65 70
75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn
His Asn Phe Leu Gln 85 90
95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr
100 105 110 Ser Arg
His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115
120 125 Tyr Pro Ile Thr Gly Pro Val
Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135
140 Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr
His Val Leu Thr 145 150 155
160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe
165 170 175 Glu Glu Ala
His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180
185 190 Thr Asp Ser Ser Pro Asn Gly Arg
Met Met Trp Thr Pro Glu Ser Asp 195 200
205 Asp Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu
Arg Gln Val 210 215 220
Glu Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala 225
230 235 240 Asp Trp Glu Leu
Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr 245
250 255 Tyr His Ser Gly Gly Tyr Leu Val Gln
Asn Pro Asp Val Phe Asp Ser 260 265
270 Lys Cys Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val
Arg Leu 275 280 285
Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly 290
295 300 Val Ser Gly Lys Tyr
Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg 305 310
315 320 Ala Val Ile Asp Pro Asp Gly Pro Ile His
Val Glu Ala Leu Ser Cys 325 330
335 Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr
Pro 340 345 350 Gly
Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro 355
360 365 Thr Thr Leu Arg Ile Phe
Arg Phe Gly Ala His Lys Trp Tyr Gly 370 375
380 17382PRTPorcine reproductive and respiratory
syndrome virus 17Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala
Arg Val 1 5 10 15
Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Pro Pro
Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35
40 45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu
His Trp Lys Val Pro Ile Gly 50 55
60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp
Leu Ser Gly 65 70 75
80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln
85 90 95 Arg Leu Val Lys
Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100
105 110 Ser Arg His Leu Arg Glu Leu Gln Val
Tyr Glu Arg Gly Cys Ser Trp 115 120
125 Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala
Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro Leu Pro
Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165
170 175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp
Lys Lys Phe Val Val Phe 180 185
190 Thr Asp Ser Ser Pro Asn Gly Met Met Trp Thr Pro Glu Ser Asp
Asp 195 200 205 Ser
Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu 210
215 220 Ile Leu Ile Arg Ser Phe
Pro Ala His His Pro Val Ser Leu Ala Asp 225 230
235 240 Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe
Ser Phe Ser Thr Tyr 245 250
255 His Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys
260 265 270 Cys Trp
Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr 275
280 285 Glu Asp Tyr Leu Ala Asn Ala
Phe Gly Tyr Gln Thr Lys Trp Gly Val 290 295
300 Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn
Gly Ile Arg Ala 305 310 315
320 Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro
325 330 335 Gln Ser Trp
Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly 340
345 350 Phe Val Arg Leu Thr Ser Leu Arg
Ile Val Pro Asn Thr Glu Pro Thr 355 360
365 Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr
Gly 370 375 380
18381PRTPorcine reproductive and respiratory syndrome virus 18Met Ser Gly
Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5
10 15 Phe Trp Asn Ala Gly Gln Val Tyr
Cys Thr Arg Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala
Ala Ile Gly 35 40 45
Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50
55 60 Ile Pro Gln Val
Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65 70
75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser
Gly Asn His Asn Phe Leu Gln 85 90
95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys
Leu Thr 100 105 110
Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp
115 120 125 Tyr Pro Ile Thr
Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130
135 140 Met His Val Ser Asp Gln Pro Phe
Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro
Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser
Ser Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser 195
200 205 Ala Asp Leu Glu Ala Leu Pro Pro
Glu Leu Glu Arg Gln Val Glu Ile 210 215
220 Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu
Ala Asp Trp 225 230 235
240 Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His
245 250 255 Ser Gly Gly Tyr
Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys 260
265 270 Trp Leu Ser Cys Phe Leu Asp Gln Pro
Ile Glu Val Arg Leu Tyr Glu 275 280
285 Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly
Val Ser 290 295 300
Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val 305
310 315 320 Ile Asp Pro Asp Gly
Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln 325
330 335 Ser Trp Ile Arg His Leu Thr Leu Asp Asp
Asp Val Thr Pro Gly Phe 340 345
350 Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr
Thr 355 360 365 Leu
Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370
375 380 19380PRTPorcine reproductive and respiratory
syndrome virus 19Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala
Arg Val 1 5 10 15
Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Pro Pro
Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35
40 45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu
His Trp Lys Val Pro Ile Gly 50 55
60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp
Leu Ser Gly 65 70 75
80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln
85 90 95 Arg Leu Val Lys
Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100
105 110 Ser Arg His Leu Arg Glu Leu Gln Val
Tyr Glu Arg Gly Cys Ser Trp 115 120
125 Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala
Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro Leu Pro
Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165
170 175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp
Lys Lys Phe Val Val Phe 180 185
190 Thr Asp Ser Ser Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser
Ala 195 200 205 Asp
Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu 210
215 220 Ile Arg Ser Phe Pro Ala
His His Pro Val Ser Leu Ala Asp Trp Glu 225 230
235 240 Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe
Ser Thr Tyr His Ser 245 250
255 Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp
260 265 270 Leu Ser
Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp 275
280 285 Tyr Leu Ala Asn Ala Phe Gly
Tyr Gln Thr Lys Trp Gly Val Ser Gly 290 295
300 Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile
Arg Ala Val Ile 305 310 315
320 Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser
325 330 335 Trp Ile Arg
His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val 340
345 350 Arg Leu Thr Ser Leu Arg Ile Val
Pro Asn Thr Glu Pro Thr Thr Leu 355 360
365 Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly
370 375 380 20379PRTPorcine reproductive
and respiratory syndrome virus 20Met Ser Gly Thr Phe Ser Arg Cys Met Cys
Thr Pro Ala Ala Arg Val 1 5 10
15 Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala
Arg 20 25 30 Ser
Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35
40 45 Leu Phe Tyr Lys Pro Lys
Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55
60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys
Cys Trp Leu Ser Gly 65 70 75
80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln
85 90 95 Arg Leu
Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100
105 110 Ser Arg His Leu Arg Glu Leu
Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120
125 Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu
Tyr Ala Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro
Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165
170 175 Glu Glu Ala His Ser Ser Val Tyr
Arg Trp Lys Lys Phe Val Val Phe 180 185
190 Thr Asp Ser Ser Met Met Trp Thr Pro Glu Ser Asp Asp
Ser Ala Asp 195 200 205
Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile 210
215 220 Arg Ser Phe Pro
Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu 225 230
235 240 Ala Glu Ser Pro Glu Asn Gly Phe Ser
Phe Ser Thr Tyr His Ser Gly 245 250
255 Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys
Trp Leu 260 265 270
Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr
275 280 285 Leu Ala Asn Ala
Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys 290
295 300 Tyr Leu Gln Arg Arg Leu Gln Val
Asn Gly Ile Arg Ala Val Ile Asp 305 310
315 320 Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys
Pro Gln Ser Trp 325 330
335 Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg
340 345 350 Leu Thr Ser
Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg 355
360 365 Ile Phe Arg Phe Gly Ala His Lys
Trp Tyr Gly 370 375 21203PRTPorcine
reproductive and respiratory syndrome virus 21Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser
Asp Asp Ser Ala Asp 20 25
30 Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu
Ile 35 40 45 Arg
Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu 50
55 60 Ala Glu Ser Pro Glu Asn
Gly Phe Ser Phe Ser Thr Tyr His Ser Gly 65 70
75 80 Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp
Ser Lys Cys Trp Leu 85 90
95 Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr
100 105 110 Leu Ala
Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys 115
120 125 Tyr Leu Gln Arg Arg Leu Gln
Val Asn Gly Ile Arg Ala Val Ile Asp 130 135
140 Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys
Pro Gln Ser Trp 145 150 155
160 Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg
165 170 175 Leu Thr Ser
Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg 180
185 190 Ile Phe Arg Phe Gly Ala His Lys
Trp Tyr Gly 195 200 22202PRTPorcine
reproductive and respiratory syndrome virus 22Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Pro Asn Gly Met Met Trp Thr Pro Glu Ser Asp
Asp Ser Ala Asp Leu 20 25
30 Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile
Arg 35 40 45 Ser
Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala 50
55 60 Glu Ser Pro Glu Asn Gly
Phe Ser Phe Ser Thr Tyr His Ser Gly Gly 65 70
75 80 Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser
Lys Cys Trp Leu Ser 85 90
95 Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu
100 105 110 Ala Asn
Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr 115
120 125 Leu Gln Arg Arg Leu Gln Val
Asn Gly Ile Arg Ala Val Ile Asp Pro 130 135
140 Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro
Gln Ser Trp Ile 145 150 155
160 Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu
165 170 175 Thr Ser Leu
Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile 180
185 190 Phe Arg Phe Gly Ala His Lys Trp
Tyr Gly 195 200 23201PRTPorcine
reproductive and respiratory syndrome virus 23Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp
Ser Ala Asp Leu Glu 20 25
30 Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg
Ser 35 40 45 Phe
Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu 50
55 60 Ser Pro Glu Asn Gly Phe
Ser Phe Ser Thr Tyr His Ser Gly Gly Tyr 65 70
75 80 Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys
Cys Trp Leu Ser Cys 85 90
95 Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu Ala
100 105 110 Asn Ala
Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu 115
120 125 Gln Arg Arg Leu Gln Val Asn
Gly Ile Arg Ala Val Ile Asp Pro Asp 130 135
140 Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln
Ser Trp Ile Arg 145 150 155
160 His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr
165 170 175 Ser Leu Arg
Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe 180
185 190 Arg Phe Gly Ala His Lys Trp Tyr
Gly 195 200 24200PRTPorcine reproductive and
respiratory syndrome virus 24Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val
Phe Thr Asp Ser Ser 1 5 10
15 Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala
20 25 30 Leu Pro
Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser Phe 35
40 45 Pro Ala His His Pro Val Ser
Leu Ala Asp Trp Glu Leu Ala Glu Ser 50 55
60 Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser
Gly Gly Tyr Leu 65 70 75
80 Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu Ser Cys Phe
85 90 95 Leu Asp Gln
Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu Ala Asn 100
105 110 Ala Phe Gly Tyr Gln Thr Lys Trp
Gly Val Ser Gly Lys Tyr Leu Gln 115 120
125 Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp
Pro Asp Gly 130 135 140
Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg His 145
150 155 160 Leu Thr Leu Asp
Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr Ser 165
170 175 Leu Arg Ile Val Pro Asn Thr Glu Pro
Thr Thr Leu Arg Ile Phe Arg 180 185
190 Phe Gly Ala His Lys Trp Tyr Gly 195
200 25199PRTPorcine reproductive and respiratory syndrome virus 25Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1
5 10 15 Met Met Trp Thr Pro Glu
Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu 20
25 30 Pro Pro Glu Leu Glu Arg Gln Val Glu Ile
Leu Ile Arg Ser Phe Pro 35 40
45 Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu
Ser Pro 50 55 60
Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly Tyr Leu Val 65
70 75 80 Gln Asn Pro Asp Val
Phe Asp Ser Lys Cys Trp Leu Ser Cys Phe Leu 85
90 95 Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu
Asp Tyr Leu Ala Asn Ala 100 105
110 Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu Gln
Arg 115 120 125 Arg
Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp Gly Pro 130
135 140 Ile His Val Glu Ala Leu
Ser Cys Pro Gln Ser Trp Ile Arg His Leu 145 150
155 160 Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val
Arg Leu Thr Ser Leu 165 170
175 Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe Arg Phe
180 185 190 Gly Ala
His Lys Trp Tyr Gly 195 2650PRTPorcine
reproductive and respiratory syndrome virus 26Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5
10 15 Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser
Asp Asp Ser Ala Asp 20 25
30 Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu
Ile 35 40 45 Arg
Ser 50 2749PRTPorcine reproductive and respiratory syndrome virus
27Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1
5 10 15 Pro Asn Gly Met
Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu 20
25 30 Glu Ala Leu Pro Pro Glu Leu Glu Arg
Gln Val Glu Ile Leu Ile Arg 35 40
45 Ser 2848PRTPorcine reproductive and respiratory
syndrome virus 28Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp
Ser Ser 1 5 10 15
Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu
20 25 30 Ala Leu Pro Pro Glu
Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35
40 45 2947PRTPorcine reproductive and
respiratory syndrome virus 29Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val
Phe Thr Asp Ser Ser 1 5 10
15 Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala
20 25 30 Leu Pro
Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35
40 45 3046PRTPorcine reproductive and
respiratory syndrome virus 30Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val
Phe Thr Asp Ser Ser 1 5 10
15 Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu
20 25 30 Pro Pro
Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35
40 45 3115110DNAPorcine reproductive and
respiratory syndrome virus 31atgatgtgta gggtattccc cctacataca cgacactact
agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct
agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg
ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca
ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa
agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg
cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt
gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac
gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca
ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtcg
gatgatgtgg acgccggaat 840ccgatgattc agccgaccta gaggcgctac cgcctgagct
agaacgtcag gtcgaaatcc 900tcattcggag ttttcctgcc catcaccctg tcagcctggc
cgactgggag ctcgctgagt 960cccctgagaa cggtttttcc ttcagcacgt accattctgg
tggttatctt gtccaaaacc 1020ccgacgtgtt tgacagcaag tgctggctct cctgtttctt
ggatcagccg atcgaagtgc 1080gcctctatga ggattatctg gctaacgctt tcggttacca
aaccaagtgg ggcgtgtctg 1140gtaagtacct ccagcgcagg cttcaagtca acggtattcg
tgctgtaatc gatcctgatg 1200gccccattca cgttgaagcg ctgtcttgcc cccaatcttg
gatcaggcac ctgactctgg 1260acgatgacgt caccccagga ttcgttcgcc taacatccct
ccgcattgtg ccgaacacag 1320agcccactac tctccgaatc tttcggtttg gagcgcataa
gtggtatggc gctgccggca 1380aacgggctcg tgccaagcgt gccgctaaaa gtgagaaggg
tccggctccc actcccaagg 1440ttgccccgcc agcccccacc tgcggaattg ttacctactc
tccaccaaca gacgggtctt 1500gcggttggca cgtccttgcc gccataatga accgaatgat
gaacggtgac ttcatgtccc 1560ctctggccca gtacaacaga ccagaggatg attgggcttc
tgattacgat cttgcccagg 1620cgattcaatg tctgcggttg cctgctacca tagttcggaa
tcgtgcctgt cccaacgcca 1680agtacctcat aaaactcaac ggagtccact gggaggtaga
ggtgaggtca gggatggccc 1740ctcgctccct tccccgcgag tgcgtagtcg gcgtttgttc
cgaaggctgt gccgcatcgc 1800cttacccaga aaacgggcta cctaaacgag cgtttgaggc
cttggcgtct gcttacagac 1860taccttccga ttgtgtcagt tctggtattg ctgactttct
tgctaacccc cctcaggaat 1920tctggactct tgacaaaatg ttgacctccc cgtcaccgga
gcggtctggt ttttccagtc 1980tgtacaaatt gctattagag gttgtcccgc agaaatgcgg
agccacggaa ggggctttca 2040cctatgctgt tgagagaatg ctgaaggatt gcccgagctc
cgaacaggcc atggcccttc 2100tggcaaaaat taaagttcca tcctcaaagg ccccgtctgt
gtccctggac gagtgtttcc 2160ctacggatgt ttcagccgat ttcgaaccag catctcagga
gaggtcccaa aattccagcg 2220ctgctgttgt cctgcattca ccgaatgcaa aagagttcga
ggaagcagct ccaggggaag 2280ttcaggaggg tggccacaag gccgtccact ctgcactccc
tgccgggggt cctaacaata 2340agcaggcaca gctggttgcc ggtgagcaac tgaagctcgg
cggttgtggt tcggtagttg 2400ggaatgcaca tgaaggtgtt ctggtcccac ctggtccaat
taatttgaca agcggggatt 2460taccctcctc aggctccatg aaagaagata tgctcaatag
ccgggaggac gaaccactgg 2520atttgtccca accagcaaca gctgtcacaa cgactcttat
gggagagcta acacccgact 2580acctaggttc tgatactggt gccctccccg tcaccgtccg
aaaatttgtc ccgacggggc 2640ctatactccg tcatgttgag cactgcagca cggggtcggg
cgatagcagt tcgcctttgg 2700atctgtctgg tgcgcaaacc ccggaccagc ctttaaatct
gtccctggcg gcttggccag 2760tgaggaccac cgcgtctgat cctggctggg tccacggtag
acgcgagcct gtctttgtaa 2820agcctcgaga tgttttctct gatggcgatt cagcccttca
gttcggggag ctttctgaat 2880ccagctctgt catcgagttt gatcgggcaa aagatgctca
ggtggctgac gcccctgtcg 2940gtctgacgac ttcggacgag gccctctccg cagtcgatcc
tttcgagttt tccgaactca 3000agcgcccacg tttctccgca caagccttaa ttgaccgagg
cggcccactt gccgatgtcc 3060atgcaaaaat aaagaaccgg gtatatgaac agtgcctcca
agcttgtgag cccggtagtc 3120gcgcaacccc agctaccagg gagtggctcg acaaaatgtg
ggagagagtg gacatgaaaa 3180cctggcgctg cacctcacag ttccaagctg gtcacattct
tgcgtccctc aaattcctcc 3240ctgacatgat tcaagacaca ccgcctcctg ttcccaggaa
gaaccgagcc agtgataaag 3300ctggcctgaa acaactagtg gcacagtggg ataggagatt
gagttcaacc ccccccccaa 3360aaccggttgg gccggtactt gaccgggtcg atcctccgcc
tacgggtacc cggcaagaag 3420acgtcacccc ctccgatggg ccaccccatg cgccggatgg
tcgagtgagt acgggtggga 3480gttggaaagg ccttatgctt tccggcaccc gtctcacggg
gtccatcagt catcgcctca 3540tgacatgggt ttttgaagtt gtctcccatc tcccagcttt
tatgctcaca cttttctcgc 3600cgcggggctc tatggctcca ggcgattggg tttttgcagg
tgttgtttta cttgctctcc 3660tgctctgtcg ttcttaccca atattcgggt gccttccctt
attgggtgtc ttttctggta 3720ctgtgcggcg tgttcgtctg ggtgtttttg gctcttggat
ggcttttgct gtatttttat 3780tctcgactcc atccaaccca gtcggttctt cttgtaacca
cgattcgccg gagtgtcatg 3840ctgagcttct ggctcttgag cagcgccaac tttgggaacc
tgtgcgcggc cttgtggtcg 3900gcccatcggg cctcttatgt gtcattcttg gcaagttact
cggtgggtca cgttatctct 3960ggcatgttct cctacgtcta tgcctgcttg cagatttggc
cctttctctt gtttatgtgg 4020tgtcccaggg gcgttgtcac aagtgttggg gaaagtgtat
aaggacagct cctgctgagg 4080tggcttttaa tgtatttcct ttctcgcgcg ccacccgtag
ttctcttgta tccttatgtg 4140atcgattcca aacgccaaaa ggagttgatc ccgtgcactt
ggcgacaggt tggcgcgggt 4200gctggcgtgg tgagagtcct atccatcaac cacaccaaaa
gcccatagct tacgccaatt 4260tggatgaaaa gaagatatct gcccaaacgg ttgttgctgt
cccatacgat cccaatcagg 4320ctatcaaatg tctgaaagtt ctgcaggcgg gaggggctat
cgtggatcag cctacgcctg 4380aggtcgttcg tgtgtccgag atccccttct cagccccatt
tttcccaaaa gttccagtca 4440acccagattg tagggtcgtg gtggattcgg acacttttgt
ggctgcggtt cgttgtggtt 4500attcgacatc acaactggtc ctgggccagg gcaactttgc
caagttaaat caaacccccc 4560ccaggaactc tatctccacc aaagcgactg gtggggcctc
ttatactttt gctgtggctc 4620aagtgtctgt gtggaccctt gttcatttcg tcctcggtct
ttggctcacg tcacctcaag 4680tgtgtggtcg aggaaccgct gacccatggt gttcaagtcc
attttcatat cctacctatg 4740gccccggggt tgtgtgctcc tctcgacttt gtgtgtctgc
tgacggggtc acccttccat 4800tgttctcagc cgtggcacaa ctctctggta gggaggtggg
gatttttatt ttagtgctcg 4860tctcctttat tgccttggcc caccgcatgg ctcttaaggc
agacatgtta gtagtctttt 4920tggctctttg tgcttatgcc tggcccatga gctcctggtt
gatctgcttc tttcctatac 4980tcttgaggtg ggttaccctt caccctctca ctatgctttg
ggtgcattca ttcttgatgt 5040tttgtctccc agcagccggc gtcctctcac tggggataac
tggcctcctc tgggcaatcg 5100gccgctttac tcaggttgcc ggaatcatta caccttatga
tatccaccag tacacctctg 5160ggccacgtgg tgcagccgct gtggccacag ccccagaagg
cacttacatg gccgccgtcc 5220ggagagctgc tttaaccggg cggactttaa tcttcacccc
gtccgcagtt ggatcccttc 5280tcgaaggtgc tttcaggact cataaaccct gccttaacac
cgtgaatgtc gtaggctcct 5340cccttggttc cggaggggtt ttcaccattg acggaaaaaa
aattgtcgtc actgctgccc 5400atgtgctgaa cggcgacaca gctagagtca ccggtgattc
ctacaaccgc atgcacactt 5460tcaagactaa tggtgactat gcctggtccc atgctgataa
ctggcagggc gctgcccctg 5520tggtcaaggt tgcgaaaggg tatcgcggtc gtgcctactg
gcaaacatca actggtgtcg 5580agcctggtgt tattgggaat gggttcgcct tctgtttcac
caactgcggc gattcggggt 5640cacccgttat ctcagaatct ggtgatctta tcggaatcca
caccggttca aacaaacttg 5700gttctggtct tgtgacaacc cctgaagggg agacctgtac
catcagagaa accaagcttt 5760ctgacctttc cagacatttc gcaggcccaa gcgttcctct
tggggacatc aaattgagtc 5820cggccatcat ccctgatgtg acatccattc cgagtgactt
ggcatcgctc ctagcttccg 5880tccctgtaat ggaaggcggc ctctcgaccg ttcaactttt
gtgtgtcttt ttcctcctct 5940ggcgcatgat gggccatgcc tggacgccca ttgttgccgt
gggcttcttt ttgctgaatg 6000aaattcttcc agcagttttg gttcgagccg tgttttcttt
tgcactcttt gtgcttgcat 6060gggccacccc ctggtctgca caagtgttga tgattagact
tctcacggca tctctcaacc 6120gcaacaaact ttctcttgcg ttctacgcac tcggaggtgt
tgttggtttg gctgctgaaa 6180tcgggacttt tgctggtaaa ttgtctgaat tgtctcaagc
tctttcgaca tactgtttct 6240tacctagggt ccttgctatg accagctgtg ttcccatcat
catcattggt ggactccatg 6300ccctcggtgt gattctgtgg ttattcaaat accggtgcct
ccacaacatg ctggttggtg 6360atggaagctt ttcaagcgct ttcttcctac ggtattttgc
agagggcaat ctcaggagag 6420gtgtttcaca gtcctgtggc atgagtaacg agtccctgac
ggctgctttg gcttgcaagt 6480tgtcacaggc tgaccttgat tttttgtcca gcttaacgaa
cttcaagtgc tttgtatctg 6540cttcaaacat gaaaaatgct gccggccagt acattgaagc
agcttatgcc agggccctgc 6600gtcaagagtt ggcctcttta gtccagattg acaaaatgaa
aggagttttg tccaagctag 6660aggcctttgc tgaaacggcc actccgtccc tcgacgtagg
tgacgtgatt gttctacttg 6720gacaacatcc tcacggatcc gttctcgata ttaatgtggg
gactgaaagg aaaactgtat 6780ccgtgcaaga gacccggagc ctaggcggct ccaagttcag
tgtttgtact gttgtgtcaa 6840acacacccgt ggacgcctta gccggtattc cactccagac
accaaccccc cttttcgaga 6900atggcccgcg tcatcgcagc gaggaggacg atcttaaagt
cgagaggatg aagaaacact 6960gcgtgtccct cggcttccac aacattaacg gtaaagttta
ctgcaagatt tgggacaagt 7020ctaccggtga cgccttttac actgatgatt cccggtacac
ccaagactat gcttttcagg 7080acaggtcagc tgactataga gacagggact acgagggtgt
gcaaaccgcc ccccaacagg 7140gatttgatcc aaagtctgaa acccctgttg gtaccgttgt
gatcggcggt attacgtaca 7200acaggtattt ggtcaaaggt aaggaggttc tggttcccaa
gcctgacaac tgccttgaag 7260ctgccaagct gtcccttgag caagctctcg ctgggatggg
ccaaacttgt gaccttacag 7320ctgccgaggt ggaaaagcta aagcgcatca ttggtcaact
tcaaggattg accactgagc 7380aggctttaaa ctgttagccg ccagcggctt gacccgctgt
ggccgcggcg gcctagttgt 7440aactgaaacg gcggtaaaaa ttgtcaaata ccacagcaga
acttttacct taggctcttt 7500agacctaaaa gtcacttccg aggtggaggt gaagaagtca
accgagcagg gccacgctgt 7560tgtggcaaac ttgtgttctg gtgtcgtctt gatgagacct
cacccaccgt cccttgtcga 7620cgttcttctg aaacccggac ttgacataac acccggcatt
caaccagggc atggggccgg 7680gaatatgggc gtggacggtt ccatttggga ttttgaaacc
gcacccacaa aggctgaact 7740cgagttatcc aagcaaataa ttcaagcatg tgaagtcagg
cgcggggatg ccccgaacct 7800ccagctccct tacaagctct atcctgttag aggggatcct
gagcggcata aaggccacct 7860catcaatacc aggtttggag acttacctta caaaactcct
caagacacca agtccgcaat 7920ccacgcggct tgttgcctgc accccaacgg ggcccccgtg
tctgatggta aatccacact 7980aggtaccact cttcaacatg gcttcgagct ttatgtccct
actgtgccct atagtgtcat 8040ggagtacctt gattcacgcc cagacacccc ttttatgtgc
actaaacacg gcacttccaa 8100ggctgctgca gaggacctcc aaaaatacga cctatccacc
caaggatttg tcctgcctgg 8160ggtcctacgc ctagtgcgca ggttcatctt tggccacatt
ggcaaggcac cgccattgtt 8220cctcccatca acttatcccg ccaagaactc catggcaggt
attaatggtc agaggttccc 8280aacaaaggat gttcaaagta tacctgaaat tgatgaaatg
tgtgcccgcg ccgtcaagga 8340gaattggcaa actgtgacac cttgcaccct caagaaacag
tattgttcta ggcccaaaac 8400caggaccatc ctgggcacta acaacttcat agccttggct
catagatcgg cgctcagtgg 8460tgttacccag gcattcatga agaaggcttg gaagtcccca
atagccttag ggaaaaacaa 8520attcaaggag ctgcattgca ctgtcgctgg caggtgcctc
gaggccgact tggcttcctg 8580tgaccgcagc acccctgcca ttgtgaggtg gttcactacc
cacctcctat atgaacttgc 8640aggatgtgaa gaatatctac ctagctatgt gcttaactgt
tgccatgacc ttgtggcgac 8700gcaggatggt gctttcacaa aacgcggtgg cctgtcgtct
ggagacccag tcaccagtgt 8760gtccaacact gtgtactcac tggtgattta tgcccagcac
atggtactat ctgccctgaa 8820aatgggtcat gaaattggcc tcaagttcct cgaagaacaa
ctcaaatttg aggaccttct 8880tgaaatccag cctatgttag tatactctga tgatcttgtc
ttgtacgcag aaaagcccac 8940cttccccaac tatcattggt gggtcgagca tcttgacctg
atgttgggct ttaaaacgga 9000cccaaagaaa accgtcataa ctgataaacc cagtttcctc
gggtgcagaa tcgaagcagg 9060gcgacagcta gtccccaatc gcgaccgcat cctggctgct
cttgcatatc acatgaaggc 9120gcagaacgcc tcagagtatt atgcgtctgc tgccgcaatc
ctgatggatt catgtgcttg 9180cattgaccac gatcctgaat ggtatgagga cctcatctgt
ggcattgccc gatgcgctcg 9240cctggacggt tatagctttc caggtccggc atttttcatg
tccatgtggg agaagctgag 9300gagtcataat gaagggaaga aattccgcca ctgcggcatc
tgcgacgcca aagccgacta 9360cgcggctgct tgtgggcttg atttgtgttt gtttcattcg
cactttcacc aacactgccc 9420tgtcactctg agctgcggtc accatgccgg ttcgaaggaa
tgttcgcagt gtcagtcacc 9480tgttggggcc ggcagatccc ctcttgatgc tgtgctggaa
caaattccat acaaacctcc 9540tcgcactgtc atcatgaagg tgggtaataa aacaacggcc
cttgatccgg ggaggtacca 9600gtcccgtcga ggtctcgttg cagtcaagag gggtattgcg
ggtaatgaag ttgatcttgc 9660tgatggagat taccaagtgg tacctcttct gccgacttgc
aaagatataa acatggtgaa 9720ggtggcttgc aacgtactac tcagcaagtt catagtaggg
ccaccaggtt ccgggaagac 9780cacctggcta ctaagtcaag ttcaggacga tgatgtcatt
tacacaccca ctcatcagac 9840catgtttgac atagttagtg ctctcaaagt ttgcaggtat
tccattccag gagcctcggg 9900actccctttc ccaccgcctg ccaggtccgg gccgtgggtt
aggctcattg ccagcgggca 9960cgtccctggc cgagtatcat acctcgatga ggccggatat
tgcaatcatc tggacattct 10020tagactgctt tccaaaacac cccttgtgtg tttgggtgac
cttcagcaac ttcacccagt 10080cggctttgat tcctattgtt atgtgttcga tcagatgcct
cagaagcagt tgaccaccat 10140ttacagattt ggccccaaca tctgcgcagc catccagcct
tgttacaggg agaaacttga 10200atctaaggct aggaacacca gggtggtttt taccacccgg
cctgtggcct ttggccaggt 10260gctgacacca tatcacaaag atcgcgtcgg ctccgcgatt
accatagact catcccaggg 10320ggccaccttt gacattgtaa cattgcatct accatcgcca
aagtccctaa ataagtcccg 10380ggcacttgtg gccatcacac gggcaagaca cgggttgttc
atttatgacc ctcacaacca 10440gctccgggag tttttcaacc taacccctga gcgcactgat
tgtaaccttg tgttcagccg 10500tggagatgag ctggtggtcc tgaatgcaga taatgcagtc
acaaccgtgg cgaaggcctt 10560agagacaggt ccaactcaat ttcgagtgtc agacccgagg
tgcaagtctc tcttagccgc 10620ttgctcggcc agtctggaag ggagctgcat gccgctaccg
caagtggcgc ataacctggg 10680gttttacttc tccccagaca gtccagtatt tgcacctctg
ccaaaagagt tggcgccaca 10740ttggccagtg gttacccatc agaataatcg ggcgtggcct
gatcgacttg tcgctagtat 10800gcgtccaatt gacgcccgct acagcaagcc gatggtcggt
gcagggtatg tggtcggacc 10860gtccaccttc cttggtactc ctggagtggt gtcatactat
ctcacactat acatcagggg 10920tgagccccag gccttgccag aaacacttgt ttcaacagga
cgtatagcca cagattgtcg 10980ggagtatctc gacgcggctg aggaagaggc agcaaaagaa
cttccccacg cgttcattgg 11040cgatgtcaaa ggcaccacag ttggggggtg tcatcacatt
acatcaaaat acctacctag 11100gtccctgcct aaagactctg ttgccgtagt tggagtaagt
tcgcctggca gggctgctaa 11160agccgtatgc accctcaccg atgtgtacct ccctgaactc
cggccatatc tgcaacctga 11220gacggcatca aaatgctgga aactcaaatt agacttcagg
gacgtccgac taatggtctg 11280gaaaggagcc accgcctact ttcaattgga agggctcaca
tggtcggcgc tgcctgacta 11340tgccaggttt attcagctgc ccaaaaacgc tgttgtatac
atcgatccgt gcataggacc 11400ggcaacagcc aatcgtaaag tcgtacgaac cacagattgg
cgggccgacc tggcagtgac 11460gccgtatgat tacggtgccc ggaacatttt gacaacagcc
tggttcgagg acctcgggcc 11520gcagtggaag attctggggt tgcagccctt taggcgggcg
tttggctttg aaaacactga 11580ggattgggca atccttgcat gctgcatgag tgacggcaag
gactacactg actataactg 11640gaattgcgtt cgacaacgcc cacacgctat ccatggacgc
gctcgtgacc atacgtacca 11700ctttgcccct ggcactgaat tgcaagtgga gctcggtaaa
ccccggctgc cacctgagca 11760agtaccgtga attcggagtg atgcaatggg gtcactgtgg
agtaaaatca gccagctgtt 11820cgtggacgcc ttcactgaat tccttgttag tgtggttgat
attgtcatct tccttgccat 11880attgtttggg ttcaccgtcg caggatggtt actggtcttt
cttctcagag tggtttgctc 11940cgcgcttctc cgttcgcgct ctgccattca ctcttccgaa
ctatcgaagg tcctatgagg 12000gcttactacc taattgcaga ccggatgttc cacaatttgc
atttaagcac cctttgggta 12060tgttttggca catgcgggtt tcccacctaa ttgatcagat
ggtctctcgc cgcatctacc 12120agaccatgga acattcaggt caagcggcct ggaagcacgt
ggtcagtgag gctactctta 12180caaaattgtc agaactcgac atagttctcc acttccaaca
cctggccgca gtggaggcgg 12240actcttgtcg cttcctcagc tcacgacttg tgatgctgaa
aaatcttgcc gttggcaatg 12300tgagcttgca gtacaacacc acgttgaacc gcgttgagct
catcctcccc acaccaggta 12360cgaggcccaa attgaccgat ttcagacaat ggctcatcag
tgtgcacgct tccatttttt 12420cctctgtagc ctcatcagtt actttgttca tagtgctttg
gcttcgaatt ccagccgtac 12480gctatgtttt tggtttccat tggcccatgg caacacgtca
ttcgagctga ccattaatta 12540cactatatgc atgccctgtc ttaccagcca agcggctcaa
caaaggctcg aacccggtcg 12600taacatgtgg tgcaaaatag gacacaccac gtgcgaggag
cgtgaccatg atgagttgtc 12660aatgtccatc ccgtccgggt acgacaacct caaacttgaa
ggttattacg cttggctggc 12720ttttttgtcc ttttcctacg cggcccaatt ccatccggag
ttgtttggaa tagggaatgt 12780gtcgcgcgtc tttgtggata aacgacacca gttcatttgt
gccgagcatg acggagataa 12840ttcaaccgta tctaccggac acaacatctc cgcatcatat
gcggcatatt atcaccacca 12900aatagacggg ggcaattggt tccatttgga atggctgcgg
ccgctctttt cctcttggct 12960agtgctcaac atatcatggt ttctgaggcg ttcgcctgca
agccctgttt ctcgacgcat 13020ctatcagata ttaagaccaa tacgaccgcg gctgccggtt
tcatggtcct tcaagacatc 13080agttgcctcc gacctcacag ggtctcagca tcgcaagaga
acattccctt cggaaagtcg 13140tcacaatgtc gtgaagccgt cggtactccc cagtacatta
cgatgactgc taatgtgacc 13200gacgaatcat atttgtacaa cgcggacttg ctaatgcttt
ccgcgtgcct tttccacgcc 13260tcagaaatga gcgagaaagg cttcaaagtt atctttggaa
acgtctccgg cgttgtttca 13320gcttgtgtca atttcacaga ttatgtggcc catgtaaccc
aacataccca acagcatcat 13380ctggtaattg atcacattcg gttactgcat ttcctgacac
catctgcaat gaggtgggct 13440acaaccattg cttgtttgtt cgccattctc ttagcgatat
gagatgttct cacaaattgg 13500ggcgtttctt gactccgcac tcttgcttct ggtggctttt
tttgctgtgt accggcttgt 13560cctggtcctt tgccgatggc aacggcaaca gctcgacacg
ccaatacata tataacttga 13620cgatatgcga gctgaatggg accgtctggt tgtccagtca
ttttgattgg gcagtcgaga 13680cctttgtgct ttacccggtg gccactcata tcctctcact
gggttttctc acaacaagcc 13740atttttttga tgcgctcggt ctcggcgctg tgtccactac
gggatttctt ggcgggcggt 13800atgtacttag cagcgtgtac ggcgcctgcg ccttcgcagc
gcttgtatgt tttgtcatcc 13860gtgctgctaa aaattgcatg gcttgccgtt atgcccgcac
ccggttcacc aacttcatcg 13920tggacgaccg ggggaagatc catcgatgga agtccccaat
agtggtagag aaattaggca 13980aagctgacat cggcggcgac cttgtcacca tcaaacatgt
tgtcctcgaa ggagtcaaag 14040ctcaaccttt gacgaggaca tcggcggagc aatgggaagc
ctagatgatt tttgcaatga 14100tcctaccgcc gcacagaagc ttgtgctggc atttagtatc
acatacacac ctataatgat 14160atacgccctc aaggtgtcac gcggccggct cctaggactg
ttacacatcc tgatatttct 14220gaactgttct ttcacgttcg gatacatgac atacgtgcac
tttcaatcca ctaaccgtgt 14280cgcgcttact atgggggcgg tcgttgccct tttgtggggc
atttacagct ttatagaatc 14340atggaagttt gtcacttcca gatgcaggtt gtgttgccta
ggccggcgat acattctggc 14400ccctgcccac cacgtagaaa gtgctgcagg cctccattca
atcccagcgt ctggtaaccg 14460agcatacgct gtgagaaagc ccggactaac atcagtgaac
ggcactctag taccaggact 14520tcggagcctc gtgttgggcg gcaaacgagc tgttaaacga
ggagtggtta acctcgtcaa 14580gtatggccgg taaaaaccag agccagaaga aaaagaaaaa
cacagctcct atggggagtg 14640gccagccagt caatcaactg tgccaattgc tgggcacaat
gataaagtcc cagcgccagc 14700ggcctagggg aggacaggcc aaaatgaaaa agcctgagaa
gccacatttc cccctagctg 14760ctgaagatga catccggcac catttcaccc agaccgagcg
ttccctttgc ttgcaatcga 14820tccagacggc cttcaatcaa ggcgcaggaa ctgcgtcgct
ttcatccagc gggaaggtca 14880gttttcaggt tgagttcatg ctgccggtcg ctcatacggt
gcgcctgatt cgcgtaactt 14940ccacatccgc cagtcagggt gcaagttaat ttgatagtta
ggtgaatggc cgcgattggc 15000gtgtggcctc tgagtcacct attcaattag ggcgatcaca
tgggggttag acttaattgg 15060cgagaaccat gtgaccgaaa ttaaaaaaaa aaaaaaaaaa
aaaaaaaaaa 151103215107DNAPorcine reproductive and
respiratory syndrome virus 32atgatgtgta gggtattccc cctacataca cgacactact
agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct
agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg
ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca
ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa
agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg
cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt
gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac
gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca
ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtat
gatgtggacg ccggaatccg 840atgattcagc cgacctagag gcgctaccgc ctgagctaga
acgtcaggtc gaaatcctca 900ttcggagttt tcctgcccat caccctgtca gcctggccga
ctgggagctc gctgagtccc 960ctgagaacgg tttttccttc agcacgtacc attctggtgg
ttatcttgtc caaaaccccg 1020acgtgtttga cagcaagtgc tggctctcct gtttcttgga
tcagccgatc gaagtgcgcc 1080tctatgagga ttatctggct aacgctttcg gttaccaaac
caagtggggc gtgtctggta 1140agtacctcca gcgcaggctt caagtcaacg gtattcgtgc
tgtaatcgat cctgatggcc 1200ccattcacgt tgaagcgctg tcttgccccc aatcttggat
caggcacctg actctggacg 1260atgacgtcac cccaggattc gttcgcctaa catccctccg
cattgtgccg aacacagagc 1320ccactactct ccgaatcttt cggtttggag cgcataagtg
gtatggcgct gccggcaaac 1380gggctcgtgc caagcgtgcc gctaaaagtg agaagggtcc
ggctcccact cccaaggttg 1440ccccgccagc ccccacctgc ggaattgtta cctactctcc
accaacagac gggtcttgcg 1500gttggcacgt ccttgccgcc ataatgaacc gaatgatgaa
cggtgacttc atgtcccctc 1560tggcccagta caacagacca gaggatgatt gggcttctga
ttacgatctt gcccaggcga 1620ttcaatgtct gcggttgcct gctaccatag ttcggaatcg
tgcctgtccc aacgccaagt 1680acctcataaa actcaacgga gtccactggg aggtagaggt
gaggtcaggg atggcccctc 1740gctcccttcc ccgcgagtgc gtagtcggcg tttgttccga
aggctgtgcc gcatcgcctt 1800acccagaaaa cgggctacct aaacgagcgt ttgaggcctt
ggcgtctgct tacagactac 1860cttccgattg tgtcagttct ggtattgctg actttcttgc
taacccccct caggaattct 1920ggactcttga caaaatgttg acctccccgt caccggagcg
gtctggtttt tccagtctgt 1980acaaattgct attagaggtt gtcccgcaga aatgcggagc
cacggaaggg gctttcacct 2040atgctgttga gagaatgctg aaggattgcc cgagctccga
acaggccatg gcccttctgg 2100caaaaattaa agttccatcc tcaaaggccc cgtctgtgtc
cctggacgag tgtttcccta 2160cggatgtttc agccgatttc gaaccagcat ctcaggagag
gtcccaaaat tccagcgctg 2220ctgttgtcct gcattcaccg aatgcaaaag agttcgagga
agcagctcca ggggaagttc 2280aggagggtgg ccacaaggcc gtccactctg cactccctgc
cgggggtcct aacaataagc 2340aggcacagct ggttgccggt gagcaactga agctcggcgg
ttgtggttcg gtagttggga 2400atgcacatga aggtgttctg gtcccacctg gtccaattaa
tttgacaagc ggggatttac 2460cctcctcagg ctccatgaaa gaagatatgc tcaatagccg
ggaggacgaa ccactggatt 2520tgtcccaacc agcaacagct gtcacaacga ctcttatggg
agagctaaca cccgactacc 2580taggttctga tactggtgcc ctccccgtca ccgtccgaaa
atttgtcccg acggggccta 2640tactccgtca tgttgagcac tgcagcacgg ggtcgggcga
tagcagttcg cctttggatc 2700tgtctggtgc gcaaaccccg gaccagcctt taaatctgtc
cctggcggct tggccagtga 2760ggaccaccgc gtctgatcct ggctgggtcc acggtagacg
cgagcctgtc tttgtaaagc 2820ctcgagatgt tttctctgat ggcgattcag cccttcagtt
cggggagctt tctgaatcca 2880gctctgtcat cgagtttgat cgggcaaaag atgctcaggt
ggctgacgcc cctgtcggtc 2940tgacgacttc ggacgaggcc ctctccgcag tcgatccttt
cgagttttcc gaactcaagc 3000gcccacgttt ctccgcacaa gccttaattg accgaggcgg
cccacttgcc gatgtccatg 3060caaaaataaa gaaccgggta tatgaacagt gcctccaagc
ttgtgagccc ggtagtcgcg 3120caaccccagc taccagggag tggctcgaca aaatgtggga
gagagtggac atgaaaacct 3180ggcgctgcac ctcacagttc caagctggtc acattcttgc
gtccctcaaa ttcctccctg 3240acatgattca agacacaccg cctcctgttc ccaggaagaa
ccgagccagt gataaagctg 3300gcctgaaaca actagtggca cagtgggata ggagattgag
ttcaaccccc cccccaaaac 3360cggttgggcc ggtacttgac cgggtcgatc ctccgcctac
gggtacccgg caagaagacg 3420tcaccccctc cgatgggcca ccccatgcgc cggatggtcg
agtgagtacg ggtgggagtt 3480ggaaaggcct tatgctttcc ggcacccgtc tcacggggtc
catcagtcat cgcctcatga 3540catgggtttt tgaagttgtc tcccatctcc cagcttttat
gctcacactt ttctcgccgc 3600ggggctctat ggctccaggc gattgggttt ttgcaggtgt
tgttttactt gctctcctgc 3660tctgtcgttc ttacccaata ttcgggtgcc ttcccttatt
gggtgtcttt tctggtactg 3720tgcggcgtgt tcgtctgggt gtttttggct cttggatggc
ttttgctgta tttttattct 3780cgactccatc caacccagtc ggttcttctt gtaaccacga
ttcgccggag tgtcatgctg 3840agcttctggc tcttgagcag cgccaacttt gggaacctgt
gcgcggcctt gtggtcggcc 3900catcgggcct cttatgtgtc attcttggca agttactcgg
tgggtcacgt tatctctggc 3960atgttctcct acgtctatgc ctgcttgcag atttggccct
ttctcttgtt tatgtggtgt 4020cccaggggcg ttgtcacaag tgttggggaa agtgtataag
gacagctcct gctgaggtgg 4080cttttaatgt atttcctttc tcgcgcgcca cccgtagttc
tcttgtatcc ttatgtgatc 4140gattccaaac gccaaaagga gttgatcccg tgcacttggc
gacaggttgg cgcgggtgct 4200ggcgtggtga gagtcctatc catcaaccac accaaaagcc
catagcttac gccaatttgg 4260atgaaaagaa gatatctgcc caaacggttg ttgctgtccc
atacgatccc aatcaggcta 4320tcaaatgtct gaaagttctg caggcgggag gggctatcgt
ggatcagcct acgcctgagg 4380tcgttcgtgt gtccgagatc cccttctcag ccccattttt
cccaaaagtt ccagtcaacc 4440cagattgtag ggtcgtggtg gattcggaca cttttgtggc
tgcggttcgt tgtggttatt 4500cgacatcaca actggtcctg ggccagggca actttgccaa
gttaaatcaa acccccccca 4560ggaactctat ctccaccaaa gcgactggtg gggcctctta
tacttttgct gtggctcaag 4620tgtctgtgtg gacccttgtt catttcgtcc tcggtctttg
gctcacgtca cctcaagtgt 4680gtggtcgagg aaccgctgac ccatggtgtt caagtccatt
ttcatatcct acctatggcc 4740ccggggttgt gtgctcctct cgactttgtg tgtctgctga
cggggtcacc cttccattgt 4800tctcagccgt ggcacaactc tctggtaggg aggtggggat
ttttatttta gtgctcgtct 4860cctttattgc cttggcccac cgcatggctc ttaaggcaga
catgttagta gtctttttgg 4920ctctttgtgc ttatgcctgg cccatgagct cctggttgat
ctgcttcttt cctatactct 4980tgaggtgggt tacccttcac cctctcacta tgctttgggt
gcattcattc ttgatgtttt 5040gtctcccagc agccggcgtc ctctcactgg ggataactgg
cctcctctgg gcaatcggcc 5100gctttactca ggttgccgga atcattacac cttatgatat
ccaccagtac acctctgggc 5160cacgtggtgc agccgctgtg gccacagccc cagaaggcac
ttacatggcc gccgtccgga 5220gagctgcttt aaccgggcgg actttaatct tcaccccgtc
cgcagttgga tcccttctcg 5280aaggtgcttt caggactcat aaaccctgcc ttaacaccgt
gaatgtcgta ggctcctccc 5340ttggttccgg aggggttttc accattgacg gaaaaaaaat
tgtcgtcact gctgcccatg 5400tgctgaacgg cgacacagct agagtcaccg gtgattccta
caaccgcatg cacactttca 5460agactaatgg tgactatgcc tggtcccatg ctgataactg
gcagggcgct gcccctgtgg 5520tcaaggttgc gaaagggtat cgcggtcgtg cctactggca
aacatcaact ggtgtcgagc 5580ctggtgttat tgggaatggg ttcgccttct gtttcaccaa
ctgcggcgat tcggggtcac 5640ccgttatctc agaatctggt gatcttatcg gaatccacac
cggttcaaac aaacttggtt 5700ctggtcttgt gacaacccct gaaggggaga cctgtaccat
cagagaaacc aagctttctg 5760acctttccag acatttcgca ggcccaagcg ttcctcttgg
ggacatcaaa ttgagtccgg 5820ccatcatccc tgatgtgaca tccattccga gtgacttggc
atcgctccta gcttccgtcc 5880ctgtaatgga aggcggcctc tcgaccgttc aacttttgtg
tgtctttttc ctcctctggc 5940gcatgatggg ccatgcctgg acgcccattg ttgccgtggg
cttctttttg ctgaatgaaa 6000ttcttccagc agttttggtt cgagccgtgt tttcttttgc
actctttgtg cttgcatggg 6060ccaccccctg gtctgcacaa gtgttgatga ttagacttct
cacggcatct ctcaaccgca 6120acaaactttc tcttgcgttc tacgcactcg gaggtgttgt
tggtttggct gctgaaatcg 6180ggacttttgc tggtaaattg tctgaattgt ctcaagctct
ttcgacatac tgtttcttac 6240ctagggtcct tgctatgacc agctgtgttc ccatcatcat
cattggtgga ctccatgccc 6300tcggtgtgat tctgtggtta ttcaaatacc ggtgcctcca
caacatgctg gttggtgatg 6360gaagcttttc aagcgctttc ttcctacggt attttgcaga
gggcaatctc aggagaggtg 6420tttcacagtc ctgtggcatg agtaacgagt ccctgacggc
tgctttggct tgcaagttgt 6480cacaggctga ccttgatttt ttgtccagct taacgaactt
caagtgcttt gtatctgctt 6540caaacatgaa aaatgctgcc ggccagtaca ttgaagcagc
ttatgccagg gccctgcgtc 6600aagagttggc ctctttagtc cagattgaca aaatgaaagg
agttttgtcc aagctagagg 6660cctttgctga aacggccact ccgtccctcg acgtaggtga
cgtgattgtt ctacttggac 6720aacatcctca cggatccgtt ctcgatatta atgtggggac
tgaaaggaaa actgtatccg 6780tgcaagagac ccggagccta ggcggctcca agttcagtgt
ttgtactgtt gtgtcaaaca 6840cacccgtgga cgccttagcc ggtattccac tccagacacc
aacccccctt ttcgagaatg 6900gcccgcgtca tcgcagcgag gaggacgatc ttaaagtcga
gaggatgaag aaacactgcg 6960tgtccctcgg cttccacaac attaacggta aagtttactg
caagatttgg gacaagtcta 7020ccggtgacgc cttttacact gatgattccc ggtacaccca
agactatgct tttcaggaca 7080ggtcagctga ctatagagac agggactacg agggtgtgca
aaccgccccc caacagggat 7140ttgatccaaa gtctgaaacc cctgttggta ccgttgtgat
cggcggtatt acgtacaaca 7200ggtatttggt caaaggtaag gaggttctgg ttcccaagcc
tgacaactgc cttgaagctg 7260ccaagctgtc ccttgagcaa gctctcgctg ggatgggcca
aacttgtgac cttacagctg 7320ccgaggtgga aaagctaaag cgcatcattg gtcaacttca
aggattgacc actgagcagg 7380ctttaaactg ttagccgcca gcggcttgac ccgctgtggc
cgcggcggcc tagttgtaac 7440tgaaacggcg gtaaaaattg tcaaatacca cagcagaact
tttaccttag gctctttaga 7500cctaaaagtc acttccgagg tggaggtgaa gaagtcaacc
gagcagggcc acgctgttgt 7560ggcaaacttg tgttctggtg tcgtcttgat gagacctcac
ccaccgtccc ttgtcgacgt 7620tcttctgaaa cccggacttg acataacacc cggcattcaa
ccagggcatg gggccgggaa 7680tatgggcgtg gacggttcca tttgggattt tgaaaccgca
cccacaaagg ctgaactcga 7740gttatccaag caaataattc aagcatgtga agtcaggcgc
ggggatgccc cgaacctcca 7800gctcccttac aagctctatc ctgttagagg ggatcctgag
cggcataaag gccacctcat 7860caataccagg tttggagact taccttacaa aactcctcaa
gacaccaagt ccgcaatcca 7920cgcggcttgt tgcctgcacc ccaacggggc ccccgtgtct
gatggtaaat ccacactagg 7980taccactctt caacatggct tcgagcttta tgtccctact
gtgccctata gtgtcatgga 8040gtaccttgat tcacgcccag acaccccttt tatgtgcact
aaacacggca cttccaaggc 8100tgctgcagag gacctccaaa aatacgacct atccacccaa
ggatttgtcc tgcctggggt 8160cctacgccta gtgcgcaggt tcatctttgg ccacattggc
aaggcaccgc cattgttcct 8220cccatcaact tatcccgcca agaactccat ggcaggtatt
aatggtcaga ggttcccaac 8280aaaggatgtt caaagtatac ctgaaattga tgaaatgtgt
gcccgcgccg tcaaggagaa 8340ttggcaaact gtgacacctt gcaccctcaa gaaacagtat
tgttctaggc ccaaaaccag 8400gaccatcctg ggcactaaca acttcatagc cttggctcat
agatcggcgc tcagtggtgt 8460tacccaggca ttcatgaaga aggcttggaa gtccccaata
gccttaggga aaaacaaatt 8520caaggagctg cattgcactg tcgctggcag gtgcctcgag
gccgacttgg cttcctgtga 8580ccgcagcacc cctgccattg tgaggtggtt cactacccac
ctcctatatg aacttgcagg 8640atgtgaagaa tatctaccta gctatgtgct taactgttgc
catgaccttg tggcgacgca 8700ggatggtgct ttcacaaaac gcggtggcct gtcgtctgga
gacccagtca ccagtgtgtc 8760caacactgtg tactcactgg tgatttatgc ccagcacatg
gtactatctg ccctgaaaat 8820gggtcatgaa attggcctca agttcctcga agaacaactc
aaatttgagg accttcttga 8880aatccagcct atgttagtat actctgatga tcttgtcttg
tacgcagaaa agcccacctt 8940ccccaactat cattggtggg tcgagcatct tgacctgatg
ttgggcttta aaacggaccc 9000aaagaaaacc gtcataactg ataaacccag tttcctcggg
tgcagaatcg aagcagggcg 9060acagctagtc cccaatcgcg accgcatcct ggctgctctt
gcatatcaca tgaaggcgca 9120gaacgcctca gagtattatg cgtctgctgc cgcaatcctg
atggattcat gtgcttgcat 9180tgaccacgat cctgaatggt atgaggacct catctgtggc
attgcccgat gcgctcgcct 9240ggacggttat agctttccag gtccggcatt tttcatgtcc
atgtgggaga agctgaggag 9300tcataatgaa gggaagaaat tccgccactg cggcatctgc
gacgccaaag ccgactacgc 9360ggctgcttgt gggcttgatt tgtgtttgtt tcattcgcac
tttcaccaac actgccctgt 9420cactctgagc tgcggtcacc atgccggttc gaaggaatgt
tcgcagtgtc agtcacctgt 9480tggggccggc agatcccctc ttgatgctgt gctggaacaa
attccataca aacctcctcg 9540cactgtcatc atgaaggtgg gtaataaaac aacggccctt
gatccgggga ggtaccagtc 9600ccgtcgaggt ctcgttgcag tcaagagggg tattgcgggt
aatgaagttg atcttgctga 9660tggagattac caagtggtac ctcttctgcc gacttgcaaa
gatataaaca tggtgaaggt 9720ggcttgcaac gtactactca gcaagttcat agtagggcca
ccaggttccg ggaagaccac 9780ctggctacta agtcaagttc aggacgatga tgtcatttac
acacccactc atcagaccat 9840gtttgacata gttagtgctc tcaaagtttg caggtattcc
attccaggag cctcgggact 9900ccctttccca ccgcctgcca ggtccgggcc gtgggttagg
ctcattgcca gcgggcacgt 9960ccctggccga gtatcatacc tcgatgaggc cggatattgc
aatcatctgg acattcttag 10020actgctttcc aaaacacccc ttgtgtgttt gggtgacctt
cagcaacttc acccagtcgg 10080ctttgattcc tattgttatg tgttcgatca gatgcctcag
aagcagttga ccaccattta 10140cagatttggc cccaacatct gcgcagccat ccagccttgt
tacagggaga aacttgaatc 10200taaggctagg aacaccaggg tggtttttac cacccggcct
gtggcctttg gccaggtgct 10260gacaccatat cacaaagatc gcgtcggctc cgcgattacc
atagactcat cccagggggc 10320cacctttgac attgtaacat tgcatctacc atcgccaaag
tccctaaata agtcccgggc 10380acttgtggcc atcacacggg caagacacgg gttgttcatt
tatgaccctc acaaccagct 10440ccgggagttt ttcaacctaa cccctgagcg cactgattgt
aaccttgtgt tcagccgtgg 10500agatgagctg gtggtcctga atgcagataa tgcagtcaca
accgtggcga aggccttaga 10560gacaggtcca actcaatttc gagtgtcaga cccgaggtgc
aagtctctct tagccgcttg 10620ctcggccagt ctggaaggga gctgcatgcc gctaccgcaa
gtggcgcata acctggggtt 10680ttacttctcc ccagacagtc cagtatttgc acctctgcca
aaagagttgg cgccacattg 10740gccagtggtt acccatcaga ataatcgggc gtggcctgat
cgacttgtcg ctagtatgcg 10800tccaattgac gcccgctaca gcaagccgat ggtcggtgca
gggtatgtgg tcggaccgtc 10860caccttcctt ggtactcctg gagtggtgtc atactatctc
acactataca tcaggggtga 10920gccccaggcc ttgccagaaa cacttgtttc aacaggacgt
atagccacag attgtcggga 10980gtatctcgac gcggctgagg aagaggcagc aaaagaactt
ccccacgcgt tcattggcga 11040tgtcaaaggc accacagttg gggggtgtca tcacattaca
tcaaaatacc tacctaggtc 11100cctgcctaaa gactctgttg ccgtagttgg agtaagttcg
cctggcaggg ctgctaaagc 11160cgtatgcacc ctcaccgatg tgtacctccc tgaactccgg
ccatatctgc aacctgagac 11220ggcatcaaaa tgctggaaac tcaaattaga cttcagggac
gtccgactaa tggtctggaa 11280aggagccacc gcctactttc aattggaagg gctcacatgg
tcggcgctgc ctgactatgc 11340caggtttatt cagctgccca aaaacgctgt tgtatacatc
gatccgtgca taggaccggc 11400aacagccaat cgtaaagtcg tacgaaccac agattggcgg
gccgacctgg cagtgacgcc 11460gtatgattac ggtgcccgga acattttgac aacagcctgg
ttcgaggacc tcgggccgca 11520gtggaagatt ctggggttgc agccctttag gcgggcgttt
ggctttgaaa acactgagga 11580ttgggcaatc cttgcatgct gcatgagtga cggcaaggac
tacactgact ataactggaa 11640ttgcgttcga caacgcccac acgctatcca tggacgcgct
cgtgaccata cgtaccactt 11700tgcccctggc actgaattgc aagtggagct cggtaaaccc
cggctgccac ctgagcaagt 11760accgtgaatt cggagtgatg caatggggtc actgtggagt
aaaatcagcc agctgttcgt 11820ggacgccttc actgaattcc ttgttagtgt ggttgatatt
gtcatcttcc ttgccatatt 11880gtttgggttc accgtcgcag gatggttact ggtctttctt
ctcagagtgg tttgctccgc 11940gcttctccgt tcgcgctctg ccattcactc ttccgaacta
tcgaaggtcc tatgagggct 12000tactacctaa ttgcagaccg gatgttccac aatttgcatt
taagcaccct ttgggtatgt 12060tttggcacat gcgggtttcc cacctaattg atcagatggt
ctctcgccgc atctaccaga 12120ccatggaaca ttcaggtcaa gcggcctgga agcacgtggt
cagtgaggct actcttacaa 12180aattgtcaga actcgacata gttctccact tccaacacct
ggccgcagtg gaggcggact 12240cttgtcgctt cctcagctca cgacttgtga tgctgaaaaa
tcttgccgtt ggcaatgtga 12300gcttgcagta caacaccacg ttgaaccgcg ttgagctcat
cctccccaca ccaggtacga 12360ggcccaaatt gaccgatttc agacaatggc tcatcagtgt
gcacgcttcc attttttcct 12420ctgtagcctc atcagttact ttgttcatag tgctttggct
tcgaattcca gccgtacgct 12480atgtttttgg tttccattgg cccatggcaa cacgtcattc
gagctgacca ttaattacac 12540tatatgcatg ccctgtctta ccagccaagc ggctcaacaa
aggctcgaac ccggtcgtaa 12600catgtggtgc aaaataggac acaccacgtg cgaggagcgt
gaccatgatg agttgtcaat 12660gtccatcccg tccgggtacg acaacctcaa acttgaaggt
tattacgctt ggctggcttt 12720tttgtccttt tcctacgcgg cccaattcca tccggagttg
tttggaatag ggaatgtgtc 12780gcgcgtcttt gtggataaac gacaccagtt catttgtgcc
gagcatgacg gagataattc 12840aaccgtatct accggacaca acatctccgc atcatatgcg
gcatattatc accaccaaat 12900agacgggggc aattggttcc atttggaatg gctgcggccg
ctcttttcct cttggctagt 12960gctcaacata tcatggtttc tgaggcgttc gcctgcaagc
cctgtttctc gacgcatcta 13020tcagatatta agaccaatac gaccgcggct gccggtttca
tggtccttca agacatcagt 13080tgcctccgac ctcacagggt ctcagcatcg caagagaaca
ttcccttcgg aaagtcgtca 13140caatgtcgtg aagccgtcgg tactccccag tacattacga
tgactgctaa tgtgaccgac 13200gaatcatatt tgtacaacgc ggacttgcta atgctttccg
cgtgcctttt ccacgcctca 13260gaaatgagcg agaaaggctt caaagttatc tttggaaacg
tctccggcgt tgtttcagct 13320tgtgtcaatt tcacagatta tgtggcccat gtaacccaac
atacccaaca gcatcatctg 13380gtaattgatc acattcggtt actgcatttc ctgacaccat
ctgcaatgag gtgggctaca 13440accattgctt gtttgttcgc cattctctta gcgatatgag
atgttctcac aaattggggc 13500gtttcttgac tccgcactct tgcttctggt ggcttttttt
gctgtgtacc ggcttgtcct 13560ggtcctttgc cgatggcaac ggcaacagct cgacacgcca
atacatatat aacttgacga 13620tatgcgagct gaatgggacc gtctggttgt ccagtcattt
tgattgggca gtcgagacct 13680ttgtgcttta cccggtggcc actcatatcc tctcactggg
ttttctcaca acaagccatt 13740tttttgatgc gctcggtctc ggcgctgtgt ccactacggg
atttcttggc gggcggtatg 13800tacttagcag cgtgtacggc gcctgcgcct tcgcagcgct
tgtatgtttt gtcatccgtg 13860ctgctaaaaa ttgcatggct tgccgttatg cccgcacccg
gttcaccaac ttcatcgtgg 13920acgaccgggg gaagatccat cgatggaagt ccccaatagt
ggtagagaaa ttaggcaaag 13980ctgacatcgg cggcgacctt gtcaccatca aacatgttgt
cctcgaagga gtcaaagctc 14040aacctttgac gaggacatcg gcggagcaat gggaagccta
gatgattttt gcaatgatcc 14100taccgccgca cagaagcttg tgctggcatt tagtatcaca
tacacaccta taatgatata 14160cgccctcaag gtgtcacgcg gccggctcct aggactgtta
cacatcctga tatttctgaa 14220ctgttctttc acgttcggat acatgacata cgtgcacttt
caatccacta accgtgtcgc 14280gcttactatg ggggcggtcg ttgccctttt gtggggcatt
tacagcttta tagaatcatg 14340gaagtttgtc acttccagat gcaggttgtg ttgcctaggc
cggcgataca ttctggcccc 14400tgcccaccac gtagaaagtg ctgcaggcct ccattcaatc
ccagcgtctg gtaaccgagc 14460atacgctgtg agaaagcccg gactaacatc agtgaacggc
actctagtac caggacttcg 14520gagcctcgtg ttgggcggca aacgagctgt taaacgagga
gtggttaacc tcgtcaagta 14580tggccggtaa aaaccagagc cagaagaaaa agaaaaacac
agctcctatg gggagtggcc 14640agccagtcaa tcaactgtgc caattgctgg gcacaatgat
aaagtcccag cgccagcggc 14700ctaggggagg acaggccaaa atgaaaaagc ctgagaagcc
acatttcccc ctagctgctg 14760aagatgacat ccggcaccat ttcacccaga ccgagcgttc
cctttgcttg caatcgatcc 14820agacggcctt caatcaaggc gcaggaactg cgtcgctttc
atccagcggg aaggtcagtt 14880ttcaggttga gttcatgctg ccggtcgctc atacggtgcg
cctgattcgc gtaacttcca 14940catccgccag tcagggtgca agttaatttg atagttaggt
gaatggccgc gattggcgtg 15000tggcctctga gtcacctatt caattagggc gatcacatgg
gggttagact taattggcga 15060gaaccatgtg accgaaatta aaaaaaaaaa aaaaaaaaaa
aaaaaaa 151073315104DNAPorcine reproductive and
respiratory syndrome virus 33atgatgtgta gggtattccc cctacataca cgacactact
agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct
agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg
ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca
ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa
agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg
cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt
gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac
gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca
ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacatgat
gtggacgccg gaatccgatg 840attcagccga cctagaggcg ctaccgcctg agctagaacg
tcaggtcgaa atcctcattc 900ggagttttcc tgcccatcac cctgtcagcc tggccgactg
ggagctcgct gagtcccctg 960agaacggttt ttccttcagc acgtaccatt ctggtggtta
tcttgtccaa aaccccgacg 1020tgtttgacag caagtgctgg ctctcctgtt tcttggatca
gccgatcgaa gtgcgcctct 1080atgaggatta tctggctaac gctttcggtt accaaaccaa
gtggggcgtg tctggtaagt 1140acctccagcg caggcttcaa gtcaacggta ttcgtgctgt
aatcgatcct gatggcccca 1200ttcacgttga agcgctgtct tgcccccaat cttggatcag
gcacctgact ctggacgatg 1260acgtcacccc aggattcgtt cgcctaacat ccctccgcat
tgtgccgaac acagagccca 1320ctactctccg aatctttcgg tttggagcgc ataagtggta
tggcgctgcc ggcaaacggg 1380ctcgtgccaa gcgtgccgct aaaagtgaga agggtccggc
tcccactccc aaggttgccc 1440cgccagcccc cacctgcgga attgttacct actctccacc
aacagacggg tcttgcggtt 1500ggcacgtcct tgccgccata atgaaccgaa tgatgaacgg
tgacttcatg tcccctctgg 1560cccagtacaa cagaccagag gatgattggg cttctgatta
cgatcttgcc caggcgattc 1620aatgtctgcg gttgcctgct accatagttc ggaatcgtgc
ctgtcccaac gccaagtacc 1680tcataaaact caacggagtc cactgggagg tagaggtgag
gtcagggatg gcccctcgct 1740cccttccccg cgagtgcgta gtcggcgttt gttccgaagg
ctgtgccgca tcgccttacc 1800cagaaaacgg gctacctaaa cgagcgtttg aggccttggc
gtctgcttac agactacctt 1860ccgattgtgt cagttctggt attgctgact ttcttgctaa
cccccctcag gaattctgga 1920ctcttgacaa aatgttgacc tccccgtcac cggagcggtc
tggtttttcc agtctgtaca 1980aattgctatt agaggttgtc ccgcagaaat gcggagccac
ggaaggggct ttcacctatg 2040ctgttgagag aatgctgaag gattgcccga gctccgaaca
ggccatggcc cttctggcaa 2100aaattaaagt tccatcctca aaggccccgt ctgtgtccct
ggacgagtgt ttccctacgg 2160atgtttcagc cgatttcgaa ccagcatctc aggagaggtc
ccaaaattcc agcgctgctg 2220ttgtcctgca ttcaccgaat gcaaaagagt tcgaggaagc
agctccaggg gaagttcagg 2280agggtggcca caaggccgtc cactctgcac tccctgccgg
gggtcctaac aataagcagg 2340cacagctggt tgccggtgag caactgaagc tcggcggttg
tggttcggta gttgggaatg 2400cacatgaagg tgttctggtc ccacctggtc caattaattt
gacaagcggg gatttaccct 2460cctcaggctc catgaaagaa gatatgctca atagccggga
ggacgaacca ctggatttgt 2520cccaaccagc aacagctgtc acaacgactc ttatgggaga
gctaacaccc gactacctag 2580gttctgatac tggtgccctc cccgtcaccg tccgaaaatt
tgtcccgacg gggcctatac 2640tccgtcatgt tgagcactgc agcacggggt cgggcgatag
cagttcgcct ttggatctgt 2700ctggtgcgca aaccccggac cagcctttaa atctgtccct
ggcggcttgg ccagtgagga 2760ccaccgcgtc tgatcctggc tgggtccacg gtagacgcga
gcctgtcttt gtaaagcctc 2820gagatgtttt ctctgatggc gattcagccc ttcagttcgg
ggagctttct gaatccagct 2880ctgtcatcga gtttgatcgg gcaaaagatg ctcaggtggc
tgacgcccct gtcggtctga 2940cgacttcgga cgaggccctc tccgcagtcg atcctttcga
gttttccgaa ctcaagcgcc 3000cacgtttctc cgcacaagcc ttaattgacc gaggcggccc
acttgccgat gtccatgcaa 3060aaataaagaa ccgggtatat gaacagtgcc tccaagcttg
tgagcccggt agtcgcgcaa 3120ccccagctac cagggagtgg ctcgacaaaa tgtgggagag
agtggacatg aaaacctggc 3180gctgcacctc acagttccaa gctggtcaca ttcttgcgtc
cctcaaattc ctccctgaca 3240tgattcaaga cacaccgcct cctgttccca ggaagaaccg
agccagtgat aaagctggcc 3300tgaaacaact agtggcacag tgggatagga gattgagttc
aacccccccc ccaaaaccgg 3360ttgggccggt acttgaccgg gtcgatcctc cgcctacggg
tacccggcaa gaagacgtca 3420ccccctccga tgggccaccc catgcgccgg atggtcgagt
gagtacgggt gggagttgga 3480aaggccttat gctttccggc acccgtctca cggggtccat
cagtcatcgc ctcatgacat 3540gggtttttga agttgtctcc catctcccag cttttatgct
cacacttttc tcgccgcggg 3600gctctatggc tccaggcgat tgggtttttg caggtgttgt
tttacttgct ctcctgctct 3660gtcgttctta cccaatattc gggtgccttc ccttattggg
tgtcttttct ggtactgtgc 3720ggcgtgttcg tctgggtgtt tttggctctt ggatggcttt
tgctgtattt ttattctcga 3780ctccatccaa cccagtcggt tcttcttgta accacgattc
gccggagtgt catgctgagc 3840ttctggctct tgagcagcgc caactttggg aacctgtgcg
cggccttgtg gtcggcccat 3900cgggcctctt atgtgtcatt cttggcaagt tactcggtgg
gtcacgttat ctctggcatg 3960ttctcctacg tctatgcctg cttgcagatt tggccctttc
tcttgtttat gtggtgtccc 4020aggggcgttg tcacaagtgt tggggaaagt gtataaggac
agctcctgct gaggtggctt 4080ttaatgtatt tcctttctcg cgcgccaccc gtagttctct
tgtatcctta tgtgatcgat 4140tccaaacgcc aaaaggagtt gatcccgtgc acttggcgac
aggttggcgc gggtgctggc 4200gtggtgagag tcctatccat caaccacacc aaaagcccat
agcttacgcc aatttggatg 4260aaaagaagat atctgcccaa acggttgttg ctgtcccata
cgatcccaat caggctatca 4320aatgtctgaa agttctgcag gcgggagggg ctatcgtgga
tcagcctacg cctgaggtcg 4380ttcgtgtgtc cgagatcccc ttctcagccc catttttccc
aaaagttcca gtcaacccag 4440attgtagggt cgtggtggat tcggacactt ttgtggctgc
ggttcgttgt ggttattcga 4500catcacaact ggtcctgggc cagggcaact ttgccaagtt
aaatcaaacc ccccccagga 4560actctatctc caccaaagcg actggtgggg cctcttatac
ttttgctgtg gctcaagtgt 4620ctgtgtggac ccttgttcat ttcgtcctcg gtctttggct
cacgtcacct caagtgtgtg 4680gtcgaggaac cgctgaccca tggtgttcaa gtccattttc
atatcctacc tatggccccg 4740gggttgtgtg ctcctctcga ctttgtgtgt ctgctgacgg
ggtcaccctt ccattgttct 4800cagccgtggc acaactctct ggtagggagg tggggatttt
tattttagtg ctcgtctcct 4860ttattgcctt ggcccaccgc atggctctta aggcagacat
gttagtagtc tttttggctc 4920tttgtgctta tgcctggccc atgagctcct ggttgatctg
cttctttcct atactcttga 4980ggtgggttac ccttcaccct ctcactatgc tttgggtgca
ttcattcttg atgttttgtc 5040tcccagcagc cggcgtcctc tcactgggga taactggcct
cctctgggca atcggccgct 5100ttactcaggt tgccggaatc attacacctt atgatatcca
ccagtacacc tctgggccac 5160gtggtgcagc cgctgtggcc acagccccag aaggcactta
catggccgcc gtccggagag 5220ctgctttaac cgggcggact ttaatcttca ccccgtccgc
agttggatcc cttctcgaag 5280gtgctttcag gactcataaa ccctgcctta acaccgtgaa
tgtcgtaggc tcctcccttg 5340gttccggagg ggttttcacc attgacggaa aaaaaattgt
cgtcactgct gcccatgtgc 5400tgaacggcga cacagctaga gtcaccggtg attcctacaa
ccgcatgcac actttcaaga 5460ctaatggtga ctatgcctgg tcccatgctg ataactggca
gggcgctgcc cctgtggtca 5520aggttgcgaa agggtatcgc ggtcgtgcct actggcaaac
atcaactggt gtcgagcctg 5580gtgttattgg gaatgggttc gccttctgtt tcaccaactg
cggcgattcg gggtcacccg 5640ttatctcaga atctggtgat cttatcggaa tccacaccgg
ttcaaacaaa cttggttctg 5700gtcttgtgac aacccctgaa ggggagacct gtaccatcag
agaaaccaag ctttctgacc 5760tttccagaca tttcgcaggc ccaagcgttc ctcttgggga
catcaaattg agtccggcca 5820tcatccctga tgtgacatcc attccgagtg acttggcatc
gctcctagct tccgtccctg 5880taatggaagg cggcctctcg accgttcaac ttttgtgtgt
ctttttcctc ctctggcgca 5940tgatgggcca tgcctggacg cccattgttg ccgtgggctt
ctttttgctg aatgaaattc 6000ttccagcagt tttggttcga gccgtgtttt cttttgcact
ctttgtgctt gcatgggcca 6060ccccctggtc tgcacaagtg ttgatgatta gacttctcac
ggcatctctc aaccgcaaca 6120aactttctct tgcgttctac gcactcggag gtgttgttgg
tttggctgct gaaatcggga 6180cttttgctgg taaattgtct gaattgtctc aagctctttc
gacatactgt ttcttaccta 6240gggtccttgc tatgaccagc tgtgttccca tcatcatcat
tggtggactc catgccctcg 6300gtgtgattct gtggttattc aaataccggt gcctccacaa
catgctggtt ggtgatggaa 6360gcttttcaag cgctttcttc ctacggtatt ttgcagaggg
caatctcagg agaggtgttt 6420cacagtcctg tggcatgagt aacgagtccc tgacggctgc
tttggcttgc aagttgtcac 6480aggctgacct tgattttttg tccagcttaa cgaacttcaa
gtgctttgta tctgcttcaa 6540acatgaaaaa tgctgccggc cagtacattg aagcagctta
tgccagggcc ctgcgtcaag 6600agttggcctc tttagtccag attgacaaaa tgaaaggagt
tttgtccaag ctagaggcct 6660ttgctgaaac ggccactccg tccctcgacg taggtgacgt
gattgttcta cttggacaac 6720atcctcacgg atccgttctc gatattaatg tggggactga
aaggaaaact gtatccgtgc 6780aagagacccg gagcctaggc ggctccaagt tcagtgtttg
tactgttgtg tcaaacacac 6840ccgtggacgc cttagccggt attccactcc agacaccaac
cccccttttc gagaatggcc 6900cgcgtcatcg cagcgaggag gacgatctta aagtcgagag
gatgaagaaa cactgcgtgt 6960ccctcggctt ccacaacatt aacggtaaag tttactgcaa
gatttgggac aagtctaccg 7020gtgacgcctt ttacactgat gattcccggt acacccaaga
ctatgctttt caggacaggt 7080cagctgacta tagagacagg gactacgagg gtgtgcaaac
cgccccccaa cagggatttg 7140atccaaagtc tgaaacccct gttggtaccg ttgtgatcgg
cggtattacg tacaacaggt 7200atttggtcaa aggtaaggag gttctggttc ccaagcctga
caactgcctt gaagctgcca 7260agctgtccct tgagcaagct ctcgctggga tgggccaaac
ttgtgacctt acagctgccg 7320aggtggaaaa gctaaagcgc atcattggtc aacttcaagg
attgaccact gagcaggctt 7380taaactgtta gccgccagcg gcttgacccg ctgtggccgc
ggcggcctag ttgtaactga 7440aacggcggta aaaattgtca aataccacag cagaactttt
accttaggct ctttagacct 7500aaaagtcact tccgaggtgg aggtgaagaa gtcaaccgag
cagggccacg ctgttgtggc 7560aaacttgtgt tctggtgtcg tcttgatgag acctcaccca
ccgtcccttg tcgacgttct 7620tctgaaaccc ggacttgaca taacacccgg cattcaacca
gggcatgggg ccgggaatat 7680gggcgtggac ggttccattt gggattttga aaccgcaccc
acaaaggctg aactcgagtt 7740atccaagcaa ataattcaag catgtgaagt caggcgcggg
gatgccccga acctccagct 7800cccttacaag ctctatcctg ttagagggga tcctgagcgg
cataaaggcc acctcatcaa 7860taccaggttt ggagacttac cttacaaaac tcctcaagac
accaagtccg caatccacgc 7920ggcttgttgc ctgcacccca acggggcccc cgtgtctgat
ggtaaatcca cactaggtac 7980cactcttcaa catggcttcg agctttatgt ccctactgtg
ccctatagtg tcatggagta 8040ccttgattca cgcccagaca ccccttttat gtgcactaaa
cacggcactt ccaaggctgc 8100tgcagaggac ctccaaaaat acgacctatc cacccaagga
tttgtcctgc ctggggtcct 8160acgcctagtg cgcaggttca tctttggcca cattggcaag
gcaccgccat tgttcctccc 8220atcaacttat cccgccaaga actccatggc aggtattaat
ggtcagaggt tcccaacaaa 8280ggatgttcaa agtatacctg aaattgatga aatgtgtgcc
cgcgccgtca aggagaattg 8340gcaaactgtg acaccttgca ccctcaagaa acagtattgt
tctaggccca aaaccaggac 8400catcctgggc actaacaact tcatagcctt ggctcataga
tcggcgctca gtggtgttac 8460ccaggcattc atgaagaagg cttggaagtc cccaatagcc
ttagggaaaa acaaattcaa 8520ggagctgcat tgcactgtcg ctggcaggtg cctcgaggcc
gacttggctt cctgtgaccg 8580cagcacccct gccattgtga ggtggttcac tacccacctc
ctatatgaac ttgcaggatg 8640tgaagaatat ctacctagct atgtgcttaa ctgttgccat
gaccttgtgg cgacgcagga 8700tggtgctttc acaaaacgcg gtggcctgtc gtctggagac
ccagtcacca gtgtgtccaa 8760cactgtgtac tcactggtga tttatgccca gcacatggta
ctatctgccc tgaaaatggg 8820tcatgaaatt ggcctcaagt tcctcgaaga acaactcaaa
tttgaggacc ttcttgaaat 8880ccagcctatg ttagtatact ctgatgatct tgtcttgtac
gcagaaaagc ccaccttccc 8940caactatcat tggtgggtcg agcatcttga cctgatgttg
ggctttaaaa cggacccaaa 9000gaaaaccgtc ataactgata aacccagttt cctcgggtgc
agaatcgaag cagggcgaca 9060gctagtcccc aatcgcgacc gcatcctggc tgctcttgca
tatcacatga aggcgcagaa 9120cgcctcagag tattatgcgt ctgctgccgc aatcctgatg
gattcatgtg cttgcattga 9180ccacgatcct gaatggtatg aggacctcat ctgtggcatt
gcccgatgcg ctcgcctgga 9240cggttatagc tttccaggtc cggcattttt catgtccatg
tgggagaagc tgaggagtca 9300taatgaaggg aagaaattcc gccactgcgg catctgcgac
gccaaagccg actacgcggc 9360tgcttgtggg cttgatttgt gtttgtttca ttcgcacttt
caccaacact gccctgtcac 9420tctgagctgc ggtcaccatg ccggttcgaa ggaatgttcg
cagtgtcagt cacctgttgg 9480ggccggcaga tcccctcttg atgctgtgct ggaacaaatt
ccatacaaac ctcctcgcac 9540tgtcatcatg aaggtgggta ataaaacaac ggcccttgat
ccggggaggt accagtcccg 9600tcgaggtctc gttgcagtca agaggggtat tgcgggtaat
gaagttgatc ttgctgatgg 9660agattaccaa gtggtacctc ttctgccgac ttgcaaagat
ataaacatgg tgaaggtggc 9720ttgcaacgta ctactcagca agttcatagt agggccacca
ggttccggga agaccacctg 9780gctactaagt caagttcagg acgatgatgt catttacaca
cccactcatc agaccatgtt 9840tgacatagtt agtgctctca aagtttgcag gtattccatt
ccaggagcct cgggactccc 9900tttcccaccg cctgccaggt ccgggccgtg ggttaggctc
attgccagcg ggcacgtccc 9960tggccgagta tcatacctcg atgaggccgg atattgcaat
catctggaca ttcttagact 10020gctttccaaa acaccccttg tgtgtttggg tgaccttcag
caacttcacc cagtcggctt 10080tgattcctat tgttatgtgt tcgatcagat gcctcagaag
cagttgacca ccatttacag 10140atttggcccc aacatctgcg cagccatcca gccttgttac
agggagaaac ttgaatctaa 10200ggctaggaac accagggtgg tttttaccac ccggcctgtg
gcctttggcc aggtgctgac 10260accatatcac aaagatcgcg tcggctccgc gattaccata
gactcatccc agggggccac 10320ctttgacatt gtaacattgc atctaccatc gccaaagtcc
ctaaataagt cccgggcact 10380tgtggccatc acacgggcaa gacacgggtt gttcatttat
gaccctcaca accagctccg 10440ggagtttttc aacctaaccc ctgagcgcac tgattgtaac
cttgtgttca gccgtggaga 10500tgagctggtg gtcctgaatg cagataatgc agtcacaacc
gtggcgaagg ccttagagac 10560aggtccaact caatttcgag tgtcagaccc gaggtgcaag
tctctcttag ccgcttgctc 10620ggccagtctg gaagggagct gcatgccgct accgcaagtg
gcgcataacc tggggtttta 10680cttctcccca gacagtccag tatttgcacc tctgccaaaa
gagttggcgc cacattggcc 10740agtggttacc catcagaata atcgggcgtg gcctgatcga
cttgtcgcta gtatgcgtcc 10800aattgacgcc cgctacagca agccgatggt cggtgcaggg
tatgtggtcg gaccgtccac 10860cttccttggt actcctggag tggtgtcata ctatctcaca
ctatacatca ggggtgagcc 10920ccaggccttg ccagaaacac ttgtttcaac aggacgtata
gccacagatt gtcgggagta 10980tctcgacgcg gctgaggaag aggcagcaaa agaacttccc
cacgcgttca ttggcgatgt 11040caaaggcacc acagttgggg ggtgtcatca cattacatca
aaatacctac ctaggtccct 11100gcctaaagac tctgttgccg tagttggagt aagttcgcct
ggcagggctg ctaaagccgt 11160atgcaccctc accgatgtgt acctccctga actccggcca
tatctgcaac ctgagacggc 11220atcaaaatgc tggaaactca aattagactt cagggacgtc
cgactaatgg tctggaaagg 11280agccaccgcc tactttcaat tggaagggct cacatggtcg
gcgctgcctg actatgccag 11340gtttattcag ctgcccaaaa acgctgttgt atacatcgat
ccgtgcatag gaccggcaac 11400agccaatcgt aaagtcgtac gaaccacaga ttggcgggcc
gacctggcag tgacgccgta 11460tgattacggt gcccggaaca ttttgacaac agcctggttc
gaggacctcg ggccgcagtg 11520gaagattctg gggttgcagc cctttaggcg ggcgtttggc
tttgaaaaca ctgaggattg 11580ggcaatcctt gcatgctgca tgagtgacgg caaggactac
actgactata actggaattg 11640cgttcgacaa cgcccacacg ctatccatgg acgcgctcgt
gaccatacgt accactttgc 11700ccctggcact gaattgcaag tggagctcgg taaaccccgg
ctgccacctg agcaagtacc 11760gtgaattcgg agtgatgcaa tggggtcact gtggagtaaa
atcagccagc tgttcgtgga 11820cgccttcact gaattccttg ttagtgtggt tgatattgtc
atcttccttg ccatattgtt 11880tgggttcacc gtcgcaggat ggttactggt ctttcttctc
agagtggttt gctccgcgct 11940tctccgttcg cgctctgcca ttcactcttc cgaactatcg
aaggtcctat gagggcttac 12000tacctaattg cagaccggat gttccacaat ttgcatttaa
gcaccctttg ggtatgtttt 12060ggcacatgcg ggtttcccac ctaattgatc agatggtctc
tcgccgcatc taccagacca 12120tggaacattc aggtcaagcg gcctggaagc acgtggtcag
tgaggctact cttacaaaat 12180tgtcagaact cgacatagtt ctccacttcc aacacctggc
cgcagtggag gcggactctt 12240gtcgcttcct cagctcacga cttgtgatgc tgaaaaatct
tgccgttggc aatgtgagct 12300tgcagtacaa caccacgttg aaccgcgttg agctcatcct
ccccacacca ggtacgaggc 12360ccaaattgac cgatttcaga caatggctca tcagtgtgca
cgcttccatt ttttcctctg 12420tagcctcatc agttactttg ttcatagtgc tttggcttcg
aattccagcc gtacgctatg 12480tttttggttt ccattggccc atggcaacac gtcattcgag
ctgaccatta attacactat 12540atgcatgccc tgtcttacca gccaagcggc tcaacaaagg
ctcgaacccg gtcgtaacat 12600gtggtgcaaa ataggacaca ccacgtgcga ggagcgtgac
catgatgagt tgtcaatgtc 12660catcccgtcc gggtacgaca acctcaaact tgaaggttat
tacgcttggc tggctttttt 12720gtccttttcc tacgcggccc aattccatcc ggagttgttt
ggaataggga atgtgtcgcg 12780cgtctttgtg gataaacgac accagttcat ttgtgccgag
catgacggag ataattcaac 12840cgtatctacc ggacacaaca tctccgcatc atatgcggca
tattatcacc accaaataga 12900cgggggcaat tggttccatt tggaatggct gcggccgctc
ttttcctctt ggctagtgct 12960caacatatca tggtttctga ggcgttcgcc tgcaagccct
gtttctcgac gcatctatca 13020gatattaaga ccaatacgac cgcggctgcc ggtttcatgg
tccttcaaga catcagttgc 13080ctccgacctc acagggtctc agcatcgcaa gagaacattc
ccttcggaaa gtcgtcacaa 13140tgtcgtgaag ccgtcggtac tccccagtac attacgatga
ctgctaatgt gaccgacgaa 13200tcatatttgt acaacgcgga cttgctaatg ctttccgcgt
gccttttcca cgcctcagaa 13260atgagcgaga aaggcttcaa agttatcttt ggaaacgtct
ccggcgttgt ttcagcttgt 13320gtcaatttca cagattatgt ggcccatgta acccaacata
cccaacagca tcatctggta 13380attgatcaca ttcggttact gcatttcctg acaccatctg
caatgaggtg ggctacaacc 13440attgcttgtt tgttcgccat tctcttagcg atatgagatg
ttctcacaaa ttggggcgtt 13500tcttgactcc gcactcttgc ttctggtggc tttttttgct
gtgtaccggc ttgtcctggt 13560cctttgccga tggcaacggc aacagctcga cacgccaata
catatataac ttgacgatat 13620gcgagctgaa tgggaccgtc tggttgtcca gtcattttga
ttgggcagtc gagacctttg 13680tgctttaccc ggtggccact catatcctct cactgggttt
tctcacaaca agccattttt 13740ttgatgcgct cggtctcggc gctgtgtcca ctacgggatt
tcttggcggg cggtatgtac 13800ttagcagcgt gtacggcgcc tgcgccttcg cagcgcttgt
atgttttgtc atccgtgctg 13860ctaaaaattg catggcttgc cgttatgccc gcacccggtt
caccaacttc atcgtggacg 13920accgggggaa gatccatcga tggaagtccc caatagtggt
agagaaatta ggcaaagctg 13980acatcggcgg cgaccttgtc accatcaaac atgttgtcct
cgaaggagtc aaagctcaac 14040ctttgacgag gacatcggcg gagcaatggg aagcctagat
gatttttgca atgatcctac 14100cgccgcacag aagcttgtgc tggcatttag tatcacatac
acacctataa tgatatacgc 14160cctcaaggtg tcacgcggcc ggctcctagg actgttacac
atcctgatat ttctgaactg 14220ttctttcacg ttcggataca tgacatacgt gcactttcaa
tccactaacc gtgtcgcgct 14280tactatgggg gcggtcgttg cccttttgtg gggcatttac
agctttatag aatcatggaa 14340gtttgtcact tccagatgca ggttgtgttg cctaggccgg
cgatacattc tggcccctgc 14400ccaccacgta gaaagtgctg caggcctcca ttcaatccca
gcgtctggta accgagcata 14460cgctgtgaga aagcccggac taacatcagt gaacggcact
ctagtaccag gacttcggag 14520cctcgtgttg ggcggcaaac gagctgttaa acgaggagtg
gttaacctcg tcaagtatgg 14580ccggtaaaaa ccagagccag aagaaaaaga aaaacacagc
tcctatgggg agtggccagc 14640cagtcaatca actgtgccaa ttgctgggca caatgataaa
gtcccagcgc cagcggccta 14700ggggaggaca ggccaaaatg aaaaagcctg agaagccaca
tttcccccta gctgctgaag 14760atgacatccg gcaccatttc acccagaccg agcgttccct
ttgcttgcaa tcgatccaga 14820cggccttcaa tcaaggcgca ggaactgcgt cgctttcatc
cagcgggaag gtcagttttc 14880aggttgagtt catgctgccg gtcgctcata cggtgcgcct
gattcgcgta acttccacat 14940ccgccagtca gggtgcaagt taatttgata gttaggtgaa
tggccgcgat tggcgtgtgg 15000cctctgagtc acctattcaa ttagggcgat cacatggggg
ttagacttaa ttggcgagaa 15060ccatgtgacc gaaattaaaa aaaaaaaaaa aaaaaaaaaa
aaaa 151043415101DNAPorcine reproductive and
respiratory syndrome virus 34atgatgtgta gggtattccc cctacataca cgacactact
agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct
agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg
ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca
ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa
agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg
cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt
gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac
gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca
ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccatgatgtg
gacgccggaa tccgatgatt 840cagccgacct agaggcgcta ccgcctgagc tagaacgtca
ggtcgaaatc ctcattcgga 900gttttcctgc ccatcaccct gtcagcctgg ccgactggga
gctcgctgag tcccctgaga 960acggtttttc cttcagcacg taccattctg gtggttatct
tgtccaaaac cccgacgtgt 1020ttgacagcaa gtgctggctc tcctgtttct tggatcagcc
gatcgaagtg cgcctctatg 1080aggattatct ggctaacgct ttcggttacc aaaccaagtg
gggcgtgtct ggtaagtacc 1140tccagcgcag gcttcaagtc aacggtattc gtgctgtaat
cgatcctgat ggccccattc 1200acgttgaagc gctgtcttgc ccccaatctt ggatcaggca
cctgactctg gacgatgacg 1260tcaccccagg attcgttcgc ctaacatccc tccgcattgt
gccgaacaca gagcccacta 1320ctctccgaat ctttcggttt ggagcgcata agtggtatgg
cgctgccggc aaacgggctc 1380gtgccaagcg tgccgctaaa agtgagaagg gtccggctcc
cactcccaag gttgccccgc 1440cagcccccac ctgcggaatt gttacctact ctccaccaac
agacgggtct tgcggttggc 1500acgtccttgc cgccataatg aaccgaatga tgaacggtga
cttcatgtcc cctctggccc 1560agtacaacag accagaggat gattgggctt ctgattacga
tcttgcccag gcgattcaat 1620gtctgcggtt gcctgctacc atagttcgga atcgtgcctg
tcccaacgcc aagtacctca 1680taaaactcaa cggagtccac tgggaggtag aggtgaggtc
agggatggcc cctcgctccc 1740ttccccgcga gtgcgtagtc ggcgtttgtt ccgaaggctg
tgccgcatcg ccttacccag 1800aaaacgggct acctaaacga gcgtttgagg ccttggcgtc
tgcttacaga ctaccttccg 1860attgtgtcag ttctggtatt gctgactttc ttgctaaccc
ccctcaggaa ttctggactc 1920ttgacaaaat gttgacctcc ccgtcaccgg agcggtctgg
tttttccagt ctgtacaaat 1980tgctattaga ggttgtcccg cagaaatgcg gagccacgga
aggggctttc acctatgctg 2040ttgagagaat gctgaaggat tgcccgagct ccgaacaggc
catggccctt ctggcaaaaa 2100ttaaagttcc atcctcaaag gccccgtctg tgtccctgga
cgagtgtttc cctacggatg 2160tttcagccga tttcgaacca gcatctcagg agaggtccca
aaattccagc gctgctgttg 2220tcctgcattc accgaatgca aaagagttcg aggaagcagc
tccaggggaa gttcaggagg 2280gtggccacaa ggccgtccac tctgcactcc ctgccggggg
tcctaacaat aagcaggcac 2340agctggttgc cggtgagcaa ctgaagctcg gcggttgtgg
ttcggtagtt gggaatgcac 2400atgaaggtgt tctggtccca cctggtccaa ttaatttgac
aagcggggat ttaccctcct 2460caggctccat gaaagaagat atgctcaata gccgggagga
cgaaccactg gatttgtccc 2520aaccagcaac agctgtcaca acgactctta tgggagagct
aacacccgac tacctaggtt 2580ctgatactgg tgccctcccc gtcaccgtcc gaaaatttgt
cccgacgggg cctatactcc 2640gtcatgttga gcactgcagc acggggtcgg gcgatagcag
ttcgcctttg gatctgtctg 2700gtgcgcaaac cccggaccag cctttaaatc tgtccctggc
ggcttggcca gtgaggacca 2760ccgcgtctga tcctggctgg gtccacggta gacgcgagcc
tgtctttgta aagcctcgag 2820atgttttctc tgatggcgat tcagcccttc agttcgggga
gctttctgaa tccagctctg 2880tcatcgagtt tgatcgggca aaagatgctc aggtggctga
cgcccctgtc ggtctgacga 2940cttcggacga ggccctctcc gcagtcgatc ctttcgagtt
ttccgaactc aagcgcccac 3000gtttctccgc acaagcctta attgaccgag gcggcccact
tgccgatgtc catgcaaaaa 3060taaagaaccg ggtatatgaa cagtgcctcc aagcttgtga
gcccggtagt cgcgcaaccc 3120cagctaccag ggagtggctc gacaaaatgt gggagagagt
ggacatgaaa acctggcgct 3180gcacctcaca gttccaagct ggtcacattc ttgcgtccct
caaattcctc cctgacatga 3240ttcaagacac accgcctcct gttcccagga agaaccgagc
cagtgataaa gctggcctga 3300aacaactagt ggcacagtgg gataggagat tgagttcaac
ccccccccca aaaccggttg 3360ggccggtact tgaccgggtc gatcctccgc ctacgggtac
ccggcaagaa gacgtcaccc 3420cctccgatgg gccaccccat gcgccggatg gtcgagtgag
tacgggtggg agttggaaag 3480gccttatgct ttccggcacc cgtctcacgg ggtccatcag
tcatcgcctc atgacatggg 3540tttttgaagt tgtctcccat ctcccagctt ttatgctcac
acttttctcg ccgcggggct 3600ctatggctcc aggcgattgg gtttttgcag gtgttgtttt
acttgctctc ctgctctgtc 3660gttcttaccc aatattcggg tgccttccct tattgggtgt
cttttctggt actgtgcggc 3720gtgttcgtct gggtgttttt ggctcttgga tggcttttgc
tgtattttta ttctcgactc 3780catccaaccc agtcggttct tcttgtaacc acgattcgcc
ggagtgtcat gctgagcttc 3840tggctcttga gcagcgccaa ctttgggaac ctgtgcgcgg
ccttgtggtc ggcccatcgg 3900gcctcttatg tgtcattctt ggcaagttac tcggtgggtc
acgttatctc tggcatgttc 3960tcctacgtct atgcctgctt gcagatttgg ccctttctct
tgtttatgtg gtgtcccagg 4020ggcgttgtca caagtgttgg ggaaagtgta taaggacagc
tcctgctgag gtggctttta 4080atgtatttcc tttctcgcgc gccacccgta gttctcttgt
atccttatgt gatcgattcc 4140aaacgccaaa aggagttgat cccgtgcact tggcgacagg
ttggcgcggg tgctggcgtg 4200gtgagagtcc tatccatcaa ccacaccaaa agcccatagc
ttacgccaat ttggatgaaa 4260agaagatatc tgcccaaacg gttgttgctg tcccatacga
tcccaatcag gctatcaaat 4320gtctgaaagt tctgcaggcg ggaggggcta tcgtggatca
gcctacgcct gaggtcgttc 4380gtgtgtccga gatccccttc tcagccccat ttttcccaaa
agttccagtc aacccagatt 4440gtagggtcgt ggtggattcg gacacttttg tggctgcggt
tcgttgtggt tattcgacat 4500cacaactggt cctgggccag ggcaactttg ccaagttaaa
tcaaaccccc cccaggaact 4560ctatctccac caaagcgact ggtggggcct cttatacttt
tgctgtggct caagtgtctg 4620tgtggaccct tgttcatttc gtcctcggtc tttggctcac
gtcacctcaa gtgtgtggtc 4680gaggaaccgc tgacccatgg tgttcaagtc cattttcata
tcctacctat ggccccgggg 4740ttgtgtgctc ctctcgactt tgtgtgtctg ctgacggggt
cacccttcca ttgttctcag 4800ccgtggcaca actctctggt agggaggtgg ggatttttat
tttagtgctc gtctccttta 4860ttgccttggc ccaccgcatg gctcttaagg cagacatgtt
agtagtcttt ttggctcttt 4920gtgcttatgc ctggcccatg agctcctggt tgatctgctt
ctttcctata ctcttgaggt 4980gggttaccct tcaccctctc actatgcttt gggtgcattc
attcttgatg ttttgtctcc 5040cagcagccgg cgtcctctca ctggggataa ctggcctcct
ctgggcaatc ggccgcttta 5100ctcaggttgc cggaatcatt acaccttatg atatccacca
gtacacctct gggccacgtg 5160gtgcagccgc tgtggccaca gccccagaag gcacttacat
ggccgccgtc cggagagctg 5220ctttaaccgg gcggacttta atcttcaccc cgtccgcagt
tggatccctt ctcgaaggtg 5280ctttcaggac tcataaaccc tgccttaaca ccgtgaatgt
cgtaggctcc tcccttggtt 5340ccggaggggt tttcaccatt gacggaaaaa aaattgtcgt
cactgctgcc catgtgctga 5400acggcgacac agctagagtc accggtgatt cctacaaccg
catgcacact ttcaagacta 5460atggtgacta tgcctggtcc catgctgata actggcaggg
cgctgcccct gtggtcaagg 5520ttgcgaaagg gtatcgcggt cgtgcctact ggcaaacatc
aactggtgtc gagcctggtg 5580ttattgggaa tgggttcgcc ttctgtttca ccaactgcgg
cgattcgggg tcacccgtta 5640tctcagaatc tggtgatctt atcggaatcc acaccggttc
aaacaaactt ggttctggtc 5700ttgtgacaac ccctgaaggg gagacctgta ccatcagaga
aaccaagctt tctgaccttt 5760ccagacattt cgcaggccca agcgttcctc ttggggacat
caaattgagt ccggccatca 5820tccctgatgt gacatccatt ccgagtgact tggcatcgct
cctagcttcc gtccctgtaa 5880tggaaggcgg cctctcgacc gttcaacttt tgtgtgtctt
tttcctcctc tggcgcatga 5940tgggccatgc ctggacgccc attgttgccg tgggcttctt
tttgctgaat gaaattcttc 6000cagcagtttt ggttcgagcc gtgttttctt ttgcactctt
tgtgcttgca tgggccaccc 6060cctggtctgc acaagtgttg atgattagac ttctcacggc
atctctcaac cgcaacaaac 6120tttctcttgc gttctacgca ctcggaggtg ttgttggttt
ggctgctgaa atcgggactt 6180ttgctggtaa attgtctgaa ttgtctcaag ctctttcgac
atactgtttc ttacctaggg 6240tccttgctat gaccagctgt gttcccatca tcatcattgg
tggactccat gccctcggtg 6300tgattctgtg gttattcaaa taccggtgcc tccacaacat
gctggttggt gatggaagct 6360tttcaagcgc tttcttccta cggtattttg cagagggcaa
tctcaggaga ggtgtttcac 6420agtcctgtgg catgagtaac gagtccctga cggctgcttt
ggcttgcaag ttgtcacagg 6480ctgaccttga ttttttgtcc agcttaacga acttcaagtg
ctttgtatct gcttcaaaca 6540tgaaaaatgc tgccggccag tacattgaag cagcttatgc
cagggccctg cgtcaagagt 6600tggcctcttt agtccagatt gacaaaatga aaggagtttt
gtccaagcta gaggcctttg 6660ctgaaacggc cactccgtcc ctcgacgtag gtgacgtgat
tgttctactt ggacaacatc 6720ctcacggatc cgttctcgat attaatgtgg ggactgaaag
gaaaactgta tccgtgcaag 6780agacccggag cctaggcggc tccaagttca gtgtttgtac
tgttgtgtca aacacacccg 6840tggacgcctt agccggtatt ccactccaga caccaacccc
ccttttcgag aatggcccgc 6900gtcatcgcag cgaggaggac gatcttaaag tcgagaggat
gaagaaacac tgcgtgtccc 6960tcggcttcca caacattaac ggtaaagttt actgcaagat
ttgggacaag tctaccggtg 7020acgcctttta cactgatgat tcccggtaca cccaagacta
tgcttttcag gacaggtcag 7080ctgactatag agacagggac tacgagggtg tgcaaaccgc
cccccaacag ggatttgatc 7140caaagtctga aacccctgtt ggtaccgttg tgatcggcgg
tattacgtac aacaggtatt 7200tggtcaaagg taaggaggtt ctggttccca agcctgacaa
ctgccttgaa gctgccaagc 7260tgtcccttga gcaagctctc gctgggatgg gccaaacttg
tgaccttaca gctgccgagg 7320tggaaaagct aaagcgcatc attggtcaac ttcaaggatt
gaccactgag caggctttaa 7380actgttagcc gccagcggct tgacccgctg tggccgcggc
ggcctagttg taactgaaac 7440ggcggtaaaa attgtcaaat accacagcag aacttttacc
ttaggctctt tagacctaaa 7500agtcacttcc gaggtggagg tgaagaagtc aaccgagcag
ggccacgctg ttgtggcaaa 7560cttgtgttct ggtgtcgtct tgatgagacc tcacccaccg
tcccttgtcg acgttcttct 7620gaaacccgga cttgacataa cacccggcat tcaaccaggg
catggggccg ggaatatggg 7680cgtggacggt tccatttggg attttgaaac cgcacccaca
aaggctgaac tcgagttatc 7740caagcaaata attcaagcat gtgaagtcag gcgcggggat
gccccgaacc tccagctccc 7800ttacaagctc tatcctgtta gaggggatcc tgagcggcat
aaaggccacc tcatcaatac 7860caggtttgga gacttacctt acaaaactcc tcaagacacc
aagtccgcaa tccacgcggc 7920ttgttgcctg caccccaacg gggcccccgt gtctgatggt
aaatccacac taggtaccac 7980tcttcaacat ggcttcgagc tttatgtccc tactgtgccc
tatagtgtca tggagtacct 8040tgattcacgc ccagacaccc cttttatgtg cactaaacac
ggcacttcca aggctgctgc 8100agaggacctc caaaaatacg acctatccac ccaaggattt
gtcctgcctg gggtcctacg 8160cctagtgcgc aggttcatct ttggccacat tggcaaggca
ccgccattgt tcctcccatc 8220aacttatccc gccaagaact ccatggcagg tattaatggt
cagaggttcc caacaaagga 8280tgttcaaagt atacctgaaa ttgatgaaat gtgtgcccgc
gccgtcaagg agaattggca 8340aactgtgaca ccttgcaccc tcaagaaaca gtattgttct
aggcccaaaa ccaggaccat 8400cctgggcact aacaacttca tagccttggc tcatagatcg
gcgctcagtg gtgttaccca 8460ggcattcatg aagaaggctt ggaagtcccc aatagcctta
gggaaaaaca aattcaagga 8520gctgcattgc actgtcgctg gcaggtgcct cgaggccgac
ttggcttcct gtgaccgcag 8580cacccctgcc attgtgaggt ggttcactac ccacctccta
tatgaacttg caggatgtga 8640agaatatcta cctagctatg tgcttaactg ttgccatgac
cttgtggcga cgcaggatgg 8700tgctttcaca aaacgcggtg gcctgtcgtc tggagaccca
gtcaccagtg tgtccaacac 8760tgtgtactca ctggtgattt atgcccagca catggtacta
tctgccctga aaatgggtca 8820tgaaattggc ctcaagttcc tcgaagaaca actcaaattt
gaggaccttc ttgaaatcca 8880gcctatgtta gtatactctg atgatcttgt cttgtacgca
gaaaagccca ccttccccaa 8940ctatcattgg tgggtcgagc atcttgacct gatgttgggc
tttaaaacgg acccaaagaa 9000aaccgtcata actgataaac ccagtttcct cgggtgcaga
atcgaagcag ggcgacagct 9060agtccccaat cgcgaccgca tcctggctgc tcttgcatat
cacatgaagg cgcagaacgc 9120ctcagagtat tatgcgtctg ctgccgcaat cctgatggat
tcatgtgctt gcattgacca 9180cgatcctgaa tggtatgagg acctcatctg tggcattgcc
cgatgcgctc gcctggacgg 9240ttatagcttt ccaggtccgg catttttcat gtccatgtgg
gagaagctga ggagtcataa 9300tgaagggaag aaattccgcc actgcggcat ctgcgacgcc
aaagccgact acgcggctgc 9360ttgtgggctt gatttgtgtt tgtttcattc gcactttcac
caacactgcc ctgtcactct 9420gagctgcggt caccatgccg gttcgaagga atgttcgcag
tgtcagtcac ctgttggggc 9480cggcagatcc cctcttgatg ctgtgctgga acaaattcca
tacaaacctc ctcgcactgt 9540catcatgaag gtgggtaata aaacaacggc ccttgatccg
gggaggtacc agtcccgtcg 9600aggtctcgtt gcagtcaaga ggggtattgc gggtaatgaa
gttgatcttg ctgatggaga 9660ttaccaagtg gtacctcttc tgccgacttg caaagatata
aacatggtga aggtggcttg 9720caacgtacta ctcagcaagt tcatagtagg gccaccaggt
tccgggaaga ccacctggct 9780actaagtcaa gttcaggacg atgatgtcat ttacacaccc
actcatcaga ccatgtttga 9840catagttagt gctctcaaag tttgcaggta ttccattcca
ggagcctcgg gactcccttt 9900cccaccgcct gccaggtccg ggccgtgggt taggctcatt
gccagcgggc acgtccctgg 9960ccgagtatca tacctcgatg aggccggata ttgcaatcat
ctggacattc ttagactgct 10020ttccaaaaca ccccttgtgt gtttgggtga ccttcagcaa
cttcacccag tcggctttga 10080ttcctattgt tatgtgttcg atcagatgcc tcagaagcag
ttgaccacca tttacagatt 10140tggccccaac atctgcgcag ccatccagcc ttgttacagg
gagaaacttg aatctaaggc 10200taggaacacc agggtggttt ttaccacccg gcctgtggcc
tttggccagg tgctgacacc 10260atatcacaaa gatcgcgtcg gctccgcgat taccatagac
tcatcccagg gggccacctt 10320tgacattgta acattgcatc taccatcgcc aaagtcccta
aataagtccc gggcacttgt 10380ggccatcaca cgggcaagac acgggttgtt catttatgac
cctcacaacc agctccggga 10440gtttttcaac ctaacccctg agcgcactga ttgtaacctt
gtgttcagcc gtggagatga 10500gctggtggtc ctgaatgcag ataatgcagt cacaaccgtg
gcgaaggcct tagagacagg 10560tccaactcaa tttcgagtgt cagacccgag gtgcaagtct
ctcttagccg cttgctcggc 10620cagtctggaa gggagctgca tgccgctacc gcaagtggcg
cataacctgg ggttttactt 10680ctccccagac agtccagtat ttgcacctct gccaaaagag
ttggcgccac attggccagt 10740ggttacccat cagaataatc gggcgtggcc tgatcgactt
gtcgctagta tgcgtccaat 10800tgacgcccgc tacagcaagc cgatggtcgg tgcagggtat
gtggtcggac cgtccacctt 10860ccttggtact cctggagtgg tgtcatacta tctcacacta
tacatcaggg gtgagcccca 10920ggccttgcca gaaacacttg tttcaacagg acgtatagcc
acagattgtc gggagtatct 10980cgacgcggct gaggaagagg cagcaaaaga acttccccac
gcgttcattg gcgatgtcaa 11040aggcaccaca gttggggggt gtcatcacat tacatcaaaa
tacctaccta ggtccctgcc 11100taaagactct gttgccgtag ttggagtaag ttcgcctggc
agggctgcta aagccgtatg 11160caccctcacc gatgtgtacc tccctgaact ccggccatat
ctgcaacctg agacggcatc 11220aaaatgctgg aaactcaaat tagacttcag ggacgtccga
ctaatggtct ggaaaggagc 11280caccgcctac tttcaattgg aagggctcac atggtcggcg
ctgcctgact atgccaggtt 11340tattcagctg cccaaaaacg ctgttgtata catcgatccg
tgcataggac cggcaacagc 11400caatcgtaaa gtcgtacgaa ccacagattg gcgggccgac
ctggcagtga cgccgtatga 11460ttacggtgcc cggaacattt tgacaacagc ctggttcgag
gacctcgggc cgcagtggaa 11520gattctgggg ttgcagccct ttaggcgggc gtttggcttt
gaaaacactg aggattgggc 11580aatccttgca tgctgcatga gtgacggcaa ggactacact
gactataact ggaattgcgt 11640tcgacaacgc ccacacgcta tccatggacg cgctcgtgac
catacgtacc actttgcccc 11700tggcactgaa ttgcaagtgg agctcggtaa accccggctg
ccacctgagc aagtaccgtg 11760aattcggagt gatgcaatgg ggtcactgtg gagtaaaatc
agccagctgt tcgtggacgc 11820cttcactgaa ttccttgtta gtgtggttga tattgtcatc
ttccttgcca tattgtttgg 11880gttcaccgtc gcaggatggt tactggtctt tcttctcaga
gtggtttgct ccgcgcttct 11940ccgttcgcgc tctgccattc actcttccga actatcgaag
gtcctatgag ggcttactac 12000ctaattgcag accggatgtt ccacaatttg catttaagca
ccctttgggt atgttttggc 12060acatgcgggt ttcccaccta attgatcaga tggtctctcg
ccgcatctac cagaccatgg 12120aacattcagg tcaagcggcc tggaagcacg tggtcagtga
ggctactctt acaaaattgt 12180cagaactcga catagttctc cacttccaac acctggccgc
agtggaggcg gactcttgtc 12240gcttcctcag ctcacgactt gtgatgctga aaaatcttgc
cgttggcaat gtgagcttgc 12300agtacaacac cacgttgaac cgcgttgagc tcatcctccc
cacaccaggt acgaggccca 12360aattgaccga tttcagacaa tggctcatca gtgtgcacgc
ttccattttt tcctctgtag 12420cctcatcagt tactttgttc atagtgcttt ggcttcgaat
tccagccgta cgctatgttt 12480ttggtttcca ttggcccatg gcaacacgtc attcgagctg
accattaatt acactatatg 12540catgccctgt cttaccagcc aagcggctca acaaaggctc
gaacccggtc gtaacatgtg 12600gtgcaaaata ggacacacca cgtgcgagga gcgtgaccat
gatgagttgt caatgtccat 12660cccgtccggg tacgacaacc tcaaacttga aggttattac
gcttggctgg cttttttgtc 12720cttttcctac gcggcccaat tccatccgga gttgtttgga
atagggaatg tgtcgcgcgt 12780ctttgtggat aaacgacacc agttcatttg tgccgagcat
gacggagata attcaaccgt 12840atctaccgga cacaacatct ccgcatcata tgcggcatat
tatcaccacc aaatagacgg 12900gggcaattgg ttccatttgg aatggctgcg gccgctcttt
tcctcttggc tagtgctcaa 12960catatcatgg tttctgaggc gttcgcctgc aagccctgtt
tctcgacgca tctatcagat 13020attaagacca atacgaccgc ggctgccggt ttcatggtcc
ttcaagacat cagttgcctc 13080cgacctcaca gggtctcagc atcgcaagag aacattccct
tcggaaagtc gtcacaatgt 13140cgtgaagccg tcggtactcc ccagtacatt acgatgactg
ctaatgtgac cgacgaatca 13200tatttgtaca acgcggactt gctaatgctt tccgcgtgcc
ttttccacgc ctcagaaatg 13260agcgagaaag gcttcaaagt tatctttgga aacgtctccg
gcgttgtttc agcttgtgtc 13320aatttcacag attatgtggc ccatgtaacc caacataccc
aacagcatca tctggtaatt 13380gatcacattc ggttactgca tttcctgaca ccatctgcaa
tgaggtgggc tacaaccatt 13440gcttgtttgt tcgccattct cttagcgata tgagatgttc
tcacaaattg gggcgtttct 13500tgactccgca ctcttgcttc tggtggcttt ttttgctgtg
taccggcttg tcctggtcct 13560ttgccgatgg caacggcaac agctcgacac gccaatacat
atataacttg acgatatgcg 13620agctgaatgg gaccgtctgg ttgtccagtc attttgattg
ggcagtcgag acctttgtgc 13680tttacccggt ggccactcat atcctctcac tgggttttct
cacaacaagc catttttttg 13740atgcgctcgg tctcggcgct gtgtccacta cgggatttct
tggcgggcgg tatgtactta 13800gcagcgtgta cggcgcctgc gccttcgcag cgcttgtatg
ttttgtcatc cgtgctgcta 13860aaaattgcat ggcttgccgt tatgcccgca cccggttcac
caacttcatc gtggacgacc 13920gggggaagat ccatcgatgg aagtccccaa tagtggtaga
gaaattaggc aaagctgaca 13980tcggcggcga ccttgtcacc atcaaacatg ttgtcctcga
aggagtcaaa gctcaacctt 14040tgacgaggac atcggcggag caatgggaag cctagatgat
ttttgcaatg atcctaccgc 14100cgcacagaag cttgtgctgg catttagtat cacatacaca
cctataatga tatacgccct 14160caaggtgtca cgcggccggc tcctaggact gttacacatc
ctgatatttc tgaactgttc 14220tttcacgttc ggatacatga catacgtgca ctttcaatcc
actaaccgtg tcgcgcttac 14280tatgggggcg gtcgttgccc ttttgtgggg catttacagc
tttatagaat catggaagtt 14340tgtcacttcc agatgcaggt tgtgttgcct aggccggcga
tacattctgg cccctgccca 14400ccacgtagaa agtgctgcag gcctccattc aatcccagcg
tctggtaacc gagcatacgc 14460tgtgagaaag cccggactaa catcagtgaa cggcactcta
gtaccaggac ttcggagcct 14520cgtgttgggc ggcaaacgag ctgttaaacg aggagtggtt
aacctcgtca agtatggccg 14580gtaaaaacca gagccagaag aaaaagaaaa acacagctcc
tatggggagt ggccagccag 14640tcaatcaact gtgccaattg ctgggcacaa tgataaagtc
ccagcgccag cggcctaggg 14700gaggacaggc caaaatgaaa aagcctgaga agccacattt
ccccctagct gctgaagatg 14760acatccggca ccatttcacc cagaccgagc gttccctttg
cttgcaatcg atccagacgg 14820ccttcaatca aggcgcagga actgcgtcgc tttcatccag
cgggaaggtc agttttcagg 14880ttgagttcat gctgccggtc gctcatacgg tgcgcctgat
tcgcgtaact tccacatccg 14940ccagtcaggg tgcaagttaa tttgatagtt aggtgaatgg
ccgcgattgg cgtgtggcct 15000ctgagtcacc tattcaatta gggcgatcac atgggggtta
gacttaattg gcgagaacca 15060tgtgaccgaa attaaaaaaa aaaaaaaaaa aaaaaaaaaa a
151013515098DNAPorcine reproductive and respiratory
syndrome virus 35atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg
taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag
gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct
cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg
acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc
tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac
ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc
ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc
ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat
gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag
cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat
tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct
ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg
tacaggtgga 780aaaaatttgt ggtcttcacg gactcctcca tgatgtggac gccggaatcc
gatgattcag 840ccgacctaga ggcgctaccg cctgagctag aacgtcaggt cgaaatcctc
attcggagtt 900ttcctgccca tcaccctgtc agcctggccg actgggagct cgctgagtcc
cctgagaacg 960gtttttcctt cagcacgtac cattctggtg gttatcttgt ccaaaacccc
gacgtgtttg 1020acagcaagtg ctggctctcc tgtttcttgg atcagccgat cgaagtgcgc
ctctatgagg 1080attatctggc taacgctttc ggttaccaaa ccaagtgggg cgtgtctggt
aagtacctcc 1140agcgcaggct tcaagtcaac ggtattcgtg ctgtaatcga tcctgatggc
cccattcacg 1200ttgaagcgct gtcttgcccc caatcttgga tcaggcacct gactctggac
gatgacgtca 1260ccccaggatt cgttcgccta acatccctcc gcattgtgcc gaacacagag
cccactactc 1320tccgaatctt tcggtttgga gcgcataagt ggtatggcgc tgccggcaaa
cgggctcgtg 1380ccaagcgtgc cgctaaaagt gagaagggtc cggctcccac tcccaaggtt
gccccgccag 1440cccccacctg cggaattgtt acctactctc caccaacaga cgggtcttgc
ggttggcacg 1500tccttgccgc cataatgaac cgaatgatga acggtgactt catgtcccct
ctggcccagt 1560acaacagacc agaggatgat tgggcttctg attacgatct tgcccaggcg
attcaatgtc 1620tgcggttgcc tgctaccata gttcggaatc gtgcctgtcc caacgccaag
tacctcataa 1680aactcaacgg agtccactgg gaggtagagg tgaggtcagg gatggcccct
cgctcccttc 1740cccgcgagtg cgtagtcggc gtttgttccg aaggctgtgc cgcatcgcct
tacccagaaa 1800acgggctacc taaacgagcg tttgaggcct tggcgtctgc ttacagacta
ccttccgatt 1860gtgtcagttc tggtattgct gactttcttg ctaacccccc tcaggaattc
tggactcttg 1920acaaaatgtt gacctccccg tcaccggagc ggtctggttt ttccagtctg
tacaaattgc 1980tattagaggt tgtcccgcag aaatgcggag ccacggaagg ggctttcacc
tatgctgttg 2040agagaatgct gaaggattgc ccgagctccg aacaggccat ggcccttctg
gcaaaaatta 2100aagttccatc ctcaaaggcc ccgtctgtgt ccctggacga gtgtttccct
acggatgttt 2160cagccgattt cgaaccagca tctcaggaga ggtcccaaaa ttccagcgct
gctgttgtcc 2220tgcattcacc gaatgcaaaa gagttcgagg aagcagctcc aggggaagtt
caggagggtg 2280gccacaaggc cgtccactct gcactccctg ccgggggtcc taacaataag
caggcacagc 2340tggttgccgg tgagcaactg aagctcggcg gttgtggttc ggtagttggg
aatgcacatg 2400aaggtgttct ggtcccacct ggtccaatta atttgacaag cggggattta
ccctcctcag 2460gctccatgaa agaagatatg ctcaatagcc gggaggacga accactggat
ttgtcccaac 2520cagcaacagc tgtcacaacg actcttatgg gagagctaac acccgactac
ctaggttctg 2580atactggtgc cctccccgtc accgtccgaa aatttgtccc gacggggcct
atactccgtc 2640atgttgagca ctgcagcacg gggtcgggcg atagcagttc gcctttggat
ctgtctggtg 2700cgcaaacccc ggaccagcct ttaaatctgt ccctggcggc ttggccagtg
aggaccaccg 2760cgtctgatcc tggctgggtc cacggtagac gcgagcctgt ctttgtaaag
cctcgagatg 2820ttttctctga tggcgattca gcccttcagt tcggggagct ttctgaatcc
agctctgtca 2880tcgagtttga tcgggcaaaa gatgctcagg tggctgacgc ccctgtcggt
ctgacgactt 2940cggacgaggc cctctccgca gtcgatcctt tcgagttttc cgaactcaag
cgcccacgtt 3000tctccgcaca agccttaatt gaccgaggcg gcccacttgc cgatgtccat
gcaaaaataa 3060agaaccgggt atatgaacag tgcctccaag cttgtgagcc cggtagtcgc
gcaaccccag 3120ctaccaggga gtggctcgac aaaatgtggg agagagtgga catgaaaacc
tggcgctgca 3180cctcacagtt ccaagctggt cacattcttg cgtccctcaa attcctccct
gacatgattc 3240aagacacacc gcctcctgtt cccaggaaga accgagccag tgataaagct
ggcctgaaac 3300aactagtggc acagtgggat aggagattga gttcaacccc ccccccaaaa
ccggttgggc 3360cggtacttga ccgggtcgat cctccgccta cgggtacccg gcaagaagac
gtcaccccct 3420ccgatgggcc accccatgcg ccggatggtc gagtgagtac gggtgggagt
tggaaaggcc 3480ttatgctttc cggcacccgt ctcacggggt ccatcagtca tcgcctcatg
acatgggttt 3540ttgaagttgt ctcccatctc ccagctttta tgctcacact tttctcgccg
cggggctcta 3600tggctccagg cgattgggtt tttgcaggtg ttgttttact tgctctcctg
ctctgtcgtt 3660cttacccaat attcgggtgc cttcccttat tgggtgtctt ttctggtact
gtgcggcgtg 3720ttcgtctggg tgtttttggc tcttggatgg cttttgctgt atttttattc
tcgactccat 3780ccaacccagt cggttcttct tgtaaccacg attcgccgga gtgtcatgct
gagcttctgg 3840ctcttgagca gcgccaactt tgggaacctg tgcgcggcct tgtggtcggc
ccatcgggcc 3900tcttatgtgt cattcttggc aagttactcg gtgggtcacg ttatctctgg
catgttctcc 3960tacgtctatg cctgcttgca gatttggccc tttctcttgt ttatgtggtg
tcccaggggc 4020gttgtcacaa gtgttgggga aagtgtataa ggacagctcc tgctgaggtg
gcttttaatg 4080tatttccttt ctcgcgcgcc acccgtagtt ctcttgtatc cttatgtgat
cgattccaaa 4140cgccaaaagg agttgatccc gtgcacttgg cgacaggttg gcgcgggtgc
tggcgtggtg 4200agagtcctat ccatcaacca caccaaaagc ccatagctta cgccaatttg
gatgaaaaga 4260agatatctgc ccaaacggtt gttgctgtcc catacgatcc caatcaggct
atcaaatgtc 4320tgaaagttct gcaggcggga ggggctatcg tggatcagcc tacgcctgag
gtcgttcgtg 4380tgtccgagat ccccttctca gccccatttt tcccaaaagt tccagtcaac
ccagattgta 4440gggtcgtggt ggattcggac acttttgtgg ctgcggttcg ttgtggttat
tcgacatcac 4500aactggtcct gggccagggc aactttgcca agttaaatca aacccccccc
aggaactcta 4560tctccaccaa agcgactggt ggggcctctt atacttttgc tgtggctcaa
gtgtctgtgt 4620ggacccttgt tcatttcgtc ctcggtcttt ggctcacgtc acctcaagtg
tgtggtcgag 4680gaaccgctga cccatggtgt tcaagtccat tttcatatcc tacctatggc
cccggggttg 4740tgtgctcctc tcgactttgt gtgtctgctg acggggtcac ccttccattg
ttctcagccg 4800tggcacaact ctctggtagg gaggtgggga tttttatttt agtgctcgtc
tcctttattg 4860ccttggccca ccgcatggct cttaaggcag acatgttagt agtctttttg
gctctttgtg 4920cttatgcctg gcccatgagc tcctggttga tctgcttctt tcctatactc
ttgaggtggg 4980ttacccttca ccctctcact atgctttggg tgcattcatt cttgatgttt
tgtctcccag 5040cagccggcgt cctctcactg gggataactg gcctcctctg ggcaatcggc
cgctttactc 5100aggttgccgg aatcattaca ccttatgata tccaccagta cacctctggg
ccacgtggtg 5160cagccgctgt ggccacagcc ccagaaggca cttacatggc cgccgtccgg
agagctgctt 5220taaccgggcg gactttaatc ttcaccccgt ccgcagttgg atcccttctc
gaaggtgctt 5280tcaggactca taaaccctgc cttaacaccg tgaatgtcgt aggctcctcc
cttggttccg 5340gaggggtttt caccattgac ggaaaaaaaa ttgtcgtcac tgctgcccat
gtgctgaacg 5400gcgacacagc tagagtcacc ggtgattcct acaaccgcat gcacactttc
aagactaatg 5460gtgactatgc ctggtcccat gctgataact ggcagggcgc tgcccctgtg
gtcaaggttg 5520cgaaagggta tcgcggtcgt gcctactggc aaacatcaac tggtgtcgag
cctggtgtta 5580ttgggaatgg gttcgccttc tgtttcacca actgcggcga ttcggggtca
cccgttatct 5640cagaatctgg tgatcttatc ggaatccaca ccggttcaaa caaacttggt
tctggtcttg 5700tgacaacccc tgaaggggag acctgtacca tcagagaaac caagctttct
gacctttcca 5760gacatttcgc aggcccaagc gttcctcttg gggacatcaa attgagtccg
gccatcatcc 5820ctgatgtgac atccattccg agtgacttgg catcgctcct agcttccgtc
cctgtaatgg 5880aaggcggcct ctcgaccgtt caacttttgt gtgtcttttt cctcctctgg
cgcatgatgg 5940gccatgcctg gacgcccatt gttgccgtgg gcttcttttt gctgaatgaa
attcttccag 6000cagttttggt tcgagccgtg ttttcttttg cactctttgt gcttgcatgg
gccaccccct 6060ggtctgcaca agtgttgatg attagacttc tcacggcatc tctcaaccgc
aacaaacttt 6120ctcttgcgtt ctacgcactc ggaggtgttg ttggtttggc tgctgaaatc
gggacttttg 6180ctggtaaatt gtctgaattg tctcaagctc tttcgacata ctgtttctta
cctagggtcc 6240ttgctatgac cagctgtgtt cccatcatca tcattggtgg actccatgcc
ctcggtgtga 6300ttctgtggtt attcaaatac cggtgcctcc acaacatgct ggttggtgat
ggaagctttt 6360caagcgcttt cttcctacgg tattttgcag agggcaatct caggagaggt
gtttcacagt 6420cctgtggcat gagtaacgag tccctgacgg ctgctttggc ttgcaagttg
tcacaggctg 6480accttgattt tttgtccagc ttaacgaact tcaagtgctt tgtatctgct
tcaaacatga 6540aaaatgctgc cggccagtac attgaagcag cttatgccag ggccctgcgt
caagagttgg 6600cctctttagt ccagattgac aaaatgaaag gagttttgtc caagctagag
gcctttgctg 6660aaacggccac tccgtccctc gacgtaggtg acgtgattgt tctacttgga
caacatcctc 6720acggatccgt tctcgatatt aatgtgggga ctgaaaggaa aactgtatcc
gtgcaagaga 6780cccggagcct aggcggctcc aagttcagtg tttgtactgt tgtgtcaaac
acacccgtgg 6840acgccttagc cggtattcca ctccagacac caacccccct tttcgagaat
ggcccgcgtc 6900atcgcagcga ggaggacgat cttaaagtcg agaggatgaa gaaacactgc
gtgtccctcg 6960gcttccacaa cattaacggt aaagtttact gcaagatttg ggacaagtct
accggtgacg 7020ccttttacac tgatgattcc cggtacaccc aagactatgc ttttcaggac
aggtcagctg 7080actatagaga cagggactac gagggtgtgc aaaccgcccc ccaacaggga
tttgatccaa 7140agtctgaaac ccctgttggt accgttgtga tcggcggtat tacgtacaac
aggtatttgg 7200tcaaaggtaa ggaggttctg gttcccaagc ctgacaactg ccttgaagct
gccaagctgt 7260cccttgagca agctctcgct gggatgggcc aaacttgtga ccttacagct
gccgaggtgg 7320aaaagctaaa gcgcatcatt ggtcaacttc aaggattgac cactgagcag
gctttaaact 7380gttagccgcc agcggcttga cccgctgtgg ccgcggcggc ctagttgtaa
ctgaaacggc 7440ggtaaaaatt gtcaaatacc acagcagaac ttttacctta ggctctttag
acctaaaagt 7500cacttccgag gtggaggtga agaagtcaac cgagcagggc cacgctgttg
tggcaaactt 7560gtgttctggt gtcgtcttga tgagacctca cccaccgtcc cttgtcgacg
ttcttctgaa 7620acccggactt gacataacac ccggcattca accagggcat ggggccggga
atatgggcgt 7680ggacggttcc atttgggatt ttgaaaccgc acccacaaag gctgaactcg
agttatccaa 7740gcaaataatt caagcatgtg aagtcaggcg cggggatgcc ccgaacctcc
agctccctta 7800caagctctat cctgttagag gggatcctga gcggcataaa ggccacctca
tcaataccag 7860gtttggagac ttaccttaca aaactcctca agacaccaag tccgcaatcc
acgcggcttg 7920ttgcctgcac cccaacgggg cccccgtgtc tgatggtaaa tccacactag
gtaccactct 7980tcaacatggc ttcgagcttt atgtccctac tgtgccctat agtgtcatgg
agtaccttga 8040ttcacgccca gacacccctt ttatgtgcac taaacacggc acttccaagg
ctgctgcaga 8100ggacctccaa aaatacgacc tatccaccca aggatttgtc ctgcctgggg
tcctacgcct 8160agtgcgcagg ttcatctttg gccacattgg caaggcaccg ccattgttcc
tcccatcaac 8220ttatcccgcc aagaactcca tggcaggtat taatggtcag aggttcccaa
caaaggatgt 8280tcaaagtata cctgaaattg atgaaatgtg tgcccgcgcc gtcaaggaga
attggcaaac 8340tgtgacacct tgcaccctca agaaacagta ttgttctagg cccaaaacca
ggaccatcct 8400gggcactaac aacttcatag ccttggctca tagatcggcg ctcagtggtg
ttacccaggc 8460attcatgaag aaggcttgga agtccccaat agccttaggg aaaaacaaat
tcaaggagct 8520gcattgcact gtcgctggca ggtgcctcga ggccgacttg gcttcctgtg
accgcagcac 8580ccctgccatt gtgaggtggt tcactaccca cctcctatat gaacttgcag
gatgtgaaga 8640atatctacct agctatgtgc ttaactgttg ccatgacctt gtggcgacgc
aggatggtgc 8700tttcacaaaa cgcggtggcc tgtcgtctgg agacccagtc accagtgtgt
ccaacactgt 8760gtactcactg gtgatttatg cccagcacat ggtactatct gccctgaaaa
tgggtcatga 8820aattggcctc aagttcctcg aagaacaact caaatttgag gaccttcttg
aaatccagcc 8880tatgttagta tactctgatg atcttgtctt gtacgcagaa aagcccacct
tccccaacta 8940tcattggtgg gtcgagcatc ttgacctgat gttgggcttt aaaacggacc
caaagaaaac 9000cgtcataact gataaaccca gtttcctcgg gtgcagaatc gaagcagggc
gacagctagt 9060ccccaatcgc gaccgcatcc tggctgctct tgcatatcac atgaaggcgc
agaacgcctc 9120agagtattat gcgtctgctg ccgcaatcct gatggattca tgtgcttgca
ttgaccacga 9180tcctgaatgg tatgaggacc tcatctgtgg cattgcccga tgcgctcgcc
tggacggtta 9240tagctttcca ggtccggcat ttttcatgtc catgtgggag aagctgagga
gtcataatga 9300agggaagaaa ttccgccact gcggcatctg cgacgccaaa gccgactacg
cggctgcttg 9360tgggcttgat ttgtgtttgt ttcattcgca ctttcaccaa cactgccctg
tcactctgag 9420ctgcggtcac catgccggtt cgaaggaatg ttcgcagtgt cagtcacctg
ttggggccgg 9480cagatcccct cttgatgctg tgctggaaca aattccatac aaacctcctc
gcactgtcat 9540catgaaggtg ggtaataaaa caacggccct tgatccgggg aggtaccagt
cccgtcgagg 9600tctcgttgca gtcaagaggg gtattgcggg taatgaagtt gatcttgctg
atggagatta 9660ccaagtggta cctcttctgc cgacttgcaa agatataaac atggtgaagg
tggcttgcaa 9720cgtactactc agcaagttca tagtagggcc accaggttcc gggaagacca
cctggctact 9780aagtcaagtt caggacgatg atgtcattta cacacccact catcagacca
tgtttgacat 9840agttagtgct ctcaaagttt gcaggtattc cattccagga gcctcgggac
tccctttccc 9900accgcctgcc aggtccgggc cgtgggttag gctcattgcc agcgggcacg
tccctggccg 9960agtatcatac ctcgatgagg ccggatattg caatcatctg gacattctta
gactgctttc 10020caaaacaccc cttgtgtgtt tgggtgacct tcagcaactt cacccagtcg
gctttgattc 10080ctattgttat gtgttcgatc agatgcctca gaagcagttg accaccattt
acagatttgg 10140ccccaacatc tgcgcagcca tccagccttg ttacagggag aaacttgaat
ctaaggctag 10200gaacaccagg gtggttttta ccacccggcc tgtggccttt ggccaggtgc
tgacaccata 10260tcacaaagat cgcgtcggct ccgcgattac catagactca tcccaggggg
ccacctttga 10320cattgtaaca ttgcatctac catcgccaaa gtccctaaat aagtcccggg
cacttgtggc 10380catcacacgg gcaagacacg ggttgttcat ttatgaccct cacaaccagc
tccgggagtt 10440tttcaaccta acccctgagc gcactgattg taaccttgtg ttcagccgtg
gagatgagct 10500ggtggtcctg aatgcagata atgcagtcac aaccgtggcg aaggccttag
agacaggtcc 10560aactcaattt cgagtgtcag acccgaggtg caagtctctc ttagccgctt
gctcggccag 10620tctggaaggg agctgcatgc cgctaccgca agtggcgcat aacctggggt
tttacttctc 10680cccagacagt ccagtatttg cacctctgcc aaaagagttg gcgccacatt
ggccagtggt 10740tacccatcag aataatcggg cgtggcctga tcgacttgtc gctagtatgc
gtccaattga 10800cgcccgctac agcaagccga tggtcggtgc agggtatgtg gtcggaccgt
ccaccttcct 10860tggtactcct ggagtggtgt catactatct cacactatac atcaggggtg
agccccaggc 10920cttgccagaa acacttgttt caacaggacg tatagccaca gattgtcggg
agtatctcga 10980cgcggctgag gaagaggcag caaaagaact tccccacgcg ttcattggcg
atgtcaaagg 11040caccacagtt ggggggtgtc atcacattac atcaaaatac ctacctaggt
ccctgcctaa 11100agactctgtt gccgtagttg gagtaagttc gcctggcagg gctgctaaag
ccgtatgcac 11160cctcaccgat gtgtacctcc ctgaactccg gccatatctg caacctgaga
cggcatcaaa 11220atgctggaaa ctcaaattag acttcaggga cgtccgacta atggtctgga
aaggagccac 11280cgcctacttt caattggaag ggctcacatg gtcggcgctg cctgactatg
ccaggtttat 11340tcagctgccc aaaaacgctg ttgtatacat cgatccgtgc ataggaccgg
caacagccaa 11400tcgtaaagtc gtacgaacca cagattggcg ggccgacctg gcagtgacgc
cgtatgatta 11460cggtgcccgg aacattttga caacagcctg gttcgaggac ctcgggccgc
agtggaagat 11520tctggggttg cagcccttta ggcgggcgtt tggctttgaa aacactgagg
attgggcaat 11580ccttgcatgc tgcatgagtg acggcaagga ctacactgac tataactgga
attgcgttcg 11640acaacgccca cacgctatcc atggacgcgc tcgtgaccat acgtaccact
ttgcccctgg 11700cactgaattg caagtggagc tcggtaaacc ccggctgcca cctgagcaag
taccgtgaat 11760tcggagtgat gcaatggggt cactgtggag taaaatcagc cagctgttcg
tggacgcctt 11820cactgaattc cttgttagtg tggttgatat tgtcatcttc cttgccatat
tgtttgggtt 11880caccgtcgca ggatggttac tggtctttct tctcagagtg gtttgctccg
cgcttctccg 11940ttcgcgctct gccattcact cttccgaact atcgaaggtc ctatgagggc
ttactaccta 12000attgcagacc ggatgttcca caatttgcat ttaagcaccc tttgggtatg
ttttggcaca 12060tgcgggtttc ccacctaatt gatcagatgg tctctcgccg catctaccag
accatggaac 12120attcaggtca agcggcctgg aagcacgtgg tcagtgaggc tactcttaca
aaattgtcag 12180aactcgacat agttctccac ttccaacacc tggccgcagt ggaggcggac
tcttgtcgct 12240tcctcagctc acgacttgtg atgctgaaaa atcttgccgt tggcaatgtg
agcttgcagt 12300acaacaccac gttgaaccgc gttgagctca tcctccccac accaggtacg
aggcccaaat 12360tgaccgattt cagacaatgg ctcatcagtg tgcacgcttc cattttttcc
tctgtagcct 12420catcagttac tttgttcata gtgctttggc ttcgaattcc agccgtacgc
tatgtttttg 12480gtttccattg gcccatggca acacgtcatt cgagctgacc attaattaca
ctatatgcat 12540gccctgtctt accagccaag cggctcaaca aaggctcgaa cccggtcgta
acatgtggtg 12600caaaatagga cacaccacgt gcgaggagcg tgaccatgat gagttgtcaa
tgtccatccc 12660gtccgggtac gacaacctca aacttgaagg ttattacgct tggctggctt
ttttgtcctt 12720ttcctacgcg gcccaattcc atccggagtt gtttggaata gggaatgtgt
cgcgcgtctt 12780tgtggataaa cgacaccagt tcatttgtgc cgagcatgac ggagataatt
caaccgtatc 12840taccggacac aacatctccg catcatatgc ggcatattat caccaccaaa
tagacggggg 12900caattggttc catttggaat ggctgcggcc gctcttttcc tcttggctag
tgctcaacat 12960atcatggttt ctgaggcgtt cgcctgcaag ccctgtttct cgacgcatct
atcagatatt 13020aagaccaata cgaccgcggc tgccggtttc atggtccttc aagacatcag
ttgcctccga 13080cctcacaggg tctcagcatc gcaagagaac attcccttcg gaaagtcgtc
acaatgtcgt 13140gaagccgtcg gtactcccca gtacattacg atgactgcta atgtgaccga
cgaatcatat 13200ttgtacaacg cggacttgct aatgctttcc gcgtgccttt tccacgcctc
agaaatgagc 13260gagaaaggct tcaaagttat ctttggaaac gtctccggcg ttgtttcagc
ttgtgtcaat 13320ttcacagatt atgtggccca tgtaacccaa catacccaac agcatcatct
ggtaattgat 13380cacattcggt tactgcattt cctgacacca tctgcaatga ggtgggctac
aaccattgct 13440tgtttgttcg ccattctctt agcgatatga gatgttctca caaattgggg
cgtttcttga 13500ctccgcactc ttgcttctgg tggctttttt tgctgtgtac cggcttgtcc
tggtcctttg 13560ccgatggcaa cggcaacagc tcgacacgcc aatacatata taacttgacg
atatgcgagc 13620tgaatgggac cgtctggttg tccagtcatt ttgattgggc agtcgagacc
tttgtgcttt 13680acccggtggc cactcatatc ctctcactgg gttttctcac aacaagccat
ttttttgatg 13740cgctcggtct cggcgctgtg tccactacgg gatttcttgg cgggcggtat
gtacttagca 13800gcgtgtacgg cgcctgcgcc ttcgcagcgc ttgtatgttt tgtcatccgt
gctgctaaaa 13860attgcatggc ttgccgttat gcccgcaccc ggttcaccaa cttcatcgtg
gacgaccggg 13920ggaagatcca tcgatggaag tccccaatag tggtagagaa attaggcaaa
gctgacatcg 13980gcggcgacct tgtcaccatc aaacatgttg tcctcgaagg agtcaaagct
caacctttga 14040cgaggacatc ggcggagcaa tgggaagcct agatgatttt tgcaatgatc
ctaccgccgc 14100acagaagctt gtgctggcat ttagtatcac atacacacct ataatgatat
acgccctcaa 14160ggtgtcacgc ggccggctcc taggactgtt acacatcctg atatttctga
actgttcttt 14220cacgttcgga tacatgacat acgtgcactt tcaatccact aaccgtgtcg
cgcttactat 14280gggggcggtc gttgcccttt tgtggggcat ttacagcttt atagaatcat
ggaagtttgt 14340cacttccaga tgcaggttgt gttgcctagg ccggcgatac attctggccc
ctgcccacca 14400cgtagaaagt gctgcaggcc tccattcaat cccagcgtct ggtaaccgag
catacgctgt 14460gagaaagccc ggactaacat cagtgaacgg cactctagta ccaggacttc
ggagcctcgt 14520gttgggcggc aaacgagctg ttaaacgagg agtggttaac ctcgtcaagt
atggccggta 14580aaaaccagag ccagaagaaa aagaaaaaca cagctcctat ggggagtggc
cagccagtca 14640atcaactgtg ccaattgctg ggcacaatga taaagtccca gcgccagcgg
cctaggggag 14700gacaggccaa aatgaaaaag cctgagaagc cacatttccc cctagctgct
gaagatgaca 14760tccggcacca tttcacccag accgagcgtt ccctttgctt gcaatcgatc
cagacggcct 14820tcaatcaagg cgcaggaact gcgtcgcttt catccagcgg gaaggtcagt
tttcaggttg 14880agttcatgct gccggtcgct catacggtgc gcctgattcg cgtaacttcc
acatccgcca 14940gtcagggtgc aagttaattt gatagttagg tgaatggccg cgattggcgt
gtggcctctg 15000agtcacctat tcaattaggg cgatcacatg ggggttagac ttaattggcg
agaaccatgt 15060gaccgaaatt aaaaaaaaaa aaaaaaaaaa aaaaaaaa
150983650PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 36Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa
Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10
15 Xaa Xaa Gly Xaa Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser
Xaa Xaa 20 25 30
Leu Glu Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa
35 40 45 Arg Ser 50
3749PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 37Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa
Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10
15 Xaa Xaa Gly Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa
Xaa Leu 20 25 30
Glu Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg
35 40 45 Ser
3848PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 38Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa
Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10
15 Xaa Xaa Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa
Leu Glu 20 25 30
Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser
35 40 45 3947PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(3)Any amino acid
39Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1
5 10 15 Xaa Xaa Met Trp
Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa 20
25 30 Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa
Xaa Xaa Leu Xaa Arg Ser 35 40
45 4046PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 40Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa
Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10
15 Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu
Xaa Leu 20 25 30
Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35
40 45 4145PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(2)..(3)Any amino acid 41Ser Xaa Xaa Tyr
Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5
10 15 Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser
Xaa Xaa Leu Glu Xaa Leu Pro 20 25
30 Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser
35 40 45 4246PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(2)Any amino acid
42Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1
5 10 15 Xaa Xaa Xaa Ser
Trp Ala Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa 20
25 30 Xaa Xaa Xaa Leu Xaa Leu Xaa Ala Xaa
Xaa Xaa Xaa Xaa Ser 35 40 45
4345PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 43Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa
Ala Val Met Xaa Val Ala Xaa 1 5 10
15 Xaa Xaa Xaa Ser Trp Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa
Xaa Xaa 20 25 30
Xaa Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35
40 45 4444PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(2)..(2)Any amino acid 44Ala Xaa Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5
10 15 Xaa Xaa Xaa Ser Gly Gly Xaa Xaa Xaa Xaa Phe
Xaa Xaa Xaa Xaa Xaa 20 25
30 Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35
40 4543PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(2)..(2)Any amino acid 45Ala Xaa Val Tyr
Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5
10 15 Xaa Xaa Xaa Gly Gly Xaa Xaa Xaa Xaa
Phe Xaa Xaa Xaa Xaa Xaa Xaa 20 25
30 Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35
40 4642PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(2)..(2)Any amino acid 46Ala Xaa Val Tyr
Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5
10 15 Xaa Xaa Gly Gly Xaa Xaa Xaa Xaa Phe
Xaa Xaa Xaa Xaa Xaa Xaa Leu 20 25
30 Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35
40 4741PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(2)..(2)Any amino acid 47Ala Xaa Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5
10 15 Xaa Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa
Xaa Xaa Xaa Leu Xaa 20 25
30 Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35
40 4850PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(17)..(17)Any amino acid 48Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Xaa Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp
Ser Ala Xaa 20 25 30
Leu Glu Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile
35 40 45 Arg Ser 50
4949PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(17)..(17)Any amino acid 49Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Xaa Asn Gly Met Met Trp Thr Pro Glu Ser Asp Asp Ser
Ala Xaa Leu 20 25 30
Glu Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg
35 40 45 Ser
5048PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(17)..(17)Any amino acid 50Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Xaa Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala
Xaa Leu Glu 20 25 30
Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser
35 40 45 5147PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(17)..(17)Any amino
acid 51Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1
5 10 15 Xaa Met Met
Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa 20
25 30 Leu Pro Pro Glu Leu Glu Arg Gln
Val Glu Ile Leu Ile Arg Ser 35 40
45 5246PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(28)..(28)Any amino acid 52Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu
Glu Xaa Leu 20 25 30
Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35
40 45 5345PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(27)..(27)Any amino acid 53Ser Ser Val
Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Met 1 5
10 15 Met Trp Thr Pro Glu Ser Asp Asp
Ser Ala Xaa Leu Glu Xaa Leu Pro 20 25
30 Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser
35 40 45 5446PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(8)..(9)Any amino acid
54Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1
5 10 15 Xaa Lys Val Ser
Trp Ala Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val 20
25 30 Pro Xaa Glu Leu Lys Leu Xaa Ala Asn
Arg Leu Xaa Thr Ser 35 40 45
5545PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(8)..(9)Any amino acid 55Ala Thr Val Tyr Asp Ile Gly Xaa Xaa
Ala Val Met Tyr Val Ala Xaa 1 5 10
15 Xaa Lys Val Ser Trp Gly Gly Xaa Glu Val Lys Phe Glu Xaa
Val Pro 20 25 30
Xaa Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35
40 45 5644PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(8)..(9)Any amino acid 56Ala Thr Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5
10 15 Xaa Lys Val Ser Gly Gly Xaa Glu Val Lys Phe
Glu Xaa Val Pro Xaa 20 25
30 Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35
40 5743PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(8)..(9)Any amino acid 57Ala Thr Val Tyr
Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5
10 15 Xaa Lys Val Gly Gly Xaa Glu Val Lys
Phe Glu Xaa Val Pro Xaa Glu 20 25
30 Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35
40 5842PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(8)..(9)Any amino acid 58Ala Thr Val Tyr
Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5
10 15 Xaa Lys Gly Gly Xaa Glu Val Lys Phe
Glu Xaa Val Pro Xaa Glu Leu 20 25
30 Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35
40 5941PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(8)..(9)Any amino acid 59Ala Thr Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5
10 15 Xaa Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val
Pro Xaa Glu Leu Lys 20 25
30 Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35
40 604PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 60Ser Xaa Xaa Tyr 1
614PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 61Ala Xaa Val Tyr 1
623PRTPorcine reproductive and respiratory syndrome virus 62Ser Phe Pro 1
634PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 63Arg Xaa Met Trp 1
643PRTPorcine reproductive and respiratory syndrome virus 64Arg Gly Gly 1
653PRTPorcine reproductive and respiratory syndrome virus 65Arg
Met Met 1 663PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 66Arg Xaa Arg 1
673PRTPorcine reproductive and respiratory syndrome virus 67Ala Pro Arg 1
684PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(3)..(3)Any amino acid 68Gly Arg Xaa Arg 1
694PRTPorcine reproductive and respiratory syndrome virus 69Trp Ala Pro
Arg 1 703PRTPorcine reproductive and respiratory syndrome
virus 70Trp Tyr Gly 1 713PRTPorcine reproductive and
respiratory syndrome virus 71Ala Gly Arg 1 723PRTPorcine
reproductive and respiratory syndrome virus 72Ala Ala Gly 1
734PRTPorcine reproductive and respiratory syndrome virus 73Glu Cys Ala
Met 1 745PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 74Ala Xaa Val Tyr Asp 1
5 754PRTPorcine reproductive and respiratory syndrome virus 75Glu Glu
Ala His 1 765PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(2)..(2)Any amino acid 76Ser Xaa Val Tyr Arg 1
5 772PRTPorcine reproductive and respiratory syndrome virus
77Pro Arg 1 783PRTPorcine reproductive and respiratory syndrome
virus 78Arg Pro Arg 1 794PRTPorcine reproductive and
respiratory syndrome virus 79Gly Arg Pro Arg 1
805PRTPorcine reproductive and respiratory syndrome virus 80Asn Gly Arg
Pro Arg 1 5 816PRTPorcine reproductive and respiratory
syndrome virus 81Pro Asn Gly Arg Pro Arg 1 5
82389PRTPorcine reproductive and respiratory syndrome virus 82Met Ser Gly
Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5
10 15 Phe Trp Asn Ala Gly Gln Val Tyr
Cys Thr Arg Cys Leu Ser Ala Arg 20 25
30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala
Ala Ile Gly 35 40 45
Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50
55 60 Ile Pro Gln Val
Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65 70
75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser
Gly Asn His Asn Phe Leu Gln 85 90
95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys
Leu Thr 100 105 110
Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp
115 120 125 Tyr Pro Ile Thr
Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130
135 140 Met His Val Ser Asp Gln Pro Phe
Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro
Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser
Ser Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu 195
200 205 Ser Asp Asp Ser Ala Asp Leu Glu
Ala Leu Pro Pro Glu Leu Glu Arg 210 215
220 Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His His
Pro Val Ser 225 230 235
240 Leu Ala Asp Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe
245 250 255 Ser Thr Tyr His
Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe 260
265 270 Asp Ser Lys Cys Trp Leu Ser Cys Phe
Leu Asp Gln Pro Ile Glu Val 275 280
285 Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln
Thr Lys 290 295 300
Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly 305
310 315 320 Ile Arg Ala Val Ile
Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu 325
330 335 Ser Cys Pro Gln Ser Trp Ile Arg His Leu
Thr Leu Asp Asp Asp Val 340 345
350 Thr Pro Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn
Thr 355 360 365 Glu
Pro Thr Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370
375 380 Gly Ala Ala Gly Lys 385
83203PRTPorcine reproductive and respiratory syndrome
virus 83Ala Asp Val Tyr Asp Ile Gly Arg Gly Ala Val Met Tyr Val Ala Gly 1
5 10 15 Gly Lys Val
Ser Trp Ala Pro Arg Gly Gly Asn Glu Val Lys Phe Glu 20
25 30 Pro Val Pro Lys Glu Leu Lys Leu
Val Ala Asn Arg Leu His Thr Ser 35 40
45 Phe Pro Pro His His Val Val Asp Met Ser Lys Phe Thr
Phe Ile Thr 50 55 60
Pro Gly Ser Gly Val Ser Met Arg Val Glu Tyr Gln Tyr Gly Cys Leu 65
70 75 80 Pro Ala Asp Thr
Val Pro Glu Gly Asn Cys Trp Trp Arg Leu Leu Asp 85
90 95 Ser Leu Pro Pro Glu Val Gln Tyr Lys
Glu Ile Arg His Ala Asn Gln 100 105
110 Phe Gly Tyr Gln Thr Lys His Gly Val Pro Gly Lys Tyr Leu
Gln Arg 115 120 125
Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Thr His Gly Pro 130
135 140 Ile Val Ile Gln Tyr
Phe Ser Val Lys Glu Ser Trp Ile Arg His Leu 145 150
155 160 Lys Leu Val Glu Glu Pro Ser Leu Pro Gly
Phe Glu Asp Leu Leu Arg 165 170
175 Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Gly Lys Asp Glu
Lys 180 185 190 Ile
Phe Arg Phe Gly Ser His Lys Trp Tyr Gly 195 200
84203PRTPorcine reproductive and respiratory syndrome virus
84Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met Tyr Val Ala Glu 1
5 10 15 Arg Lys Val Ser
Trp Ala Pro Arg Gly Gly Asp Glu Val Lys Phe Glu 20
25 30 Ala Val Pro Gly Glu Leu Lys Leu Ile
Ala Asn Arg Leu Arg Thr Ser 35 40
45 Phe Pro Pro His His Thr Val Asp Met Ser Lys Phe Ala Phe
Thr Ala 50 55 60
Pro Gly Cys Gly Val Ser Met Arg Val Glu Arg Gln His Gly Cys Leu 65
70 75 80 Pro Ala Asp Thr Val
Pro Glu Gly Asn Cys Trp Trp Ser Leu Phe Asp 85
90 95 Leu Leu Pro Leu Glu Val Gln Asn Lys Glu
Ile Arg His Ala Asn Gln 100 105
110 Phe Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys Tyr Leu Gln
Arg 115 120 125 Arg
Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Leu Asn Gly Pro 130
135 140 Ile Val Val Gln Tyr Phe
Ser Val Lys Glu Ser Trp Ile Arg His Leu 145 150
155 160 Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe
Glu Asp Leu Leu Arg 165 170
175 Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Asp Lys Glu Glu Lys
180 185 190 Ile Phe
Arg Phe Gly Ser His Lys Trp Tyr Gly 195 200
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